Subject: antigens and Infectious spleen and kidney necrosis virus / Subject term: Infectious spleen and kidney necrosis virus

Author:: Xiaozhe Fu; Ningqiu Li; Qiang Lin; Huizhi Guo; Lihui Liu; Zhibin Huang; Shuqin Wu
Source:: Fish & shellfish immunology 2015 v.46 no.2 pp. 200-205
ISSN:: 1050-4648
Subject:: Escherichia coli; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; adjuvants; antigens; aquaculture industry; fish; gene expression regulation; genes; immunoglobulin M; interferon regulatory factor-7; mortality; muscle tissues; polyclonal antibodies; rabbits; transcription (genetics); vaccination; vaccines
Abstract:: ... Infectious spleen and kidney necrosis virus (ISKNV) has caused significant loss in the Mandarin fish (Siniperca chuatsi) aquaculture industry. Vaccination is an important measure to prevent fatal ISKNV infection. In this study, the ORF086 gene encoding an early protein helicase of ISKNV was cloned into the prokaryotic pET32a (+) and eukaryotic pcDNA3.1 (+) expression vectors and designated as pET0 ...
DOI:: 10.1016/j.fsi.2015.05.052
: https://doi.org/10.1016/j.fsi.2015.05.052

Author:: Tomomasa Matsuyama; Natsumi Sano; Tomokazu Takano; Takamitsu Sakai; Motoshige Yasuike; Atushi Fujiwara; Yasuhiko Kawato; Jun Kurita; Kazunori Yoshida; Yukinori Shimada; Chihaya Nakayasu
Source:: Vaccine 2018 v.36 no.19 pp. 2643-2649
ISSN:: 0264-410X
Subject:: Infectious spleen and kidney necrosis virus; Seriola dumerili; Seriola quinqueradiata; antibodies; antigens; blood serum; case studies; enzyme-linked immunosorbent assay; fish; genes; immune response; models; pathogens; prediction; protective effect; recombinant vaccines; screening; sorbents; vaccine development
Abstract:: ... Predicting antigens that would be protective is crucial for the development of recombinant vaccine using genome based vaccine development, also known as reverse vaccinology. High-throughput antigen screening is effective for identifying vaccine target genes, particularly for pathogens for which minimal antigenicity data exist. Using red sea bream iridovirus (RSIV) as a research model, we developed ...
DOI:: 10.1016/j.vaccine.2018.03.059
: https://doi.org/10.1016/j.vaccine.2018.03.059

Author:: Woo Ju Kwon; Jae Chan Choi; Suhee Hong; Young Chul Kim; Min Gyeong Jeong; Joon Gyu Min; Joon Bum Jeong; Kwang Il Kim; Hyun Do Jeong
Source:: Vaccine 2020 v.38 no.51 pp. 8107-8115
ISSN:: 0264-410X
Subject:: Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Pagrus major; adjuvants; aluminum hydroxide; antigens; bream; cell lines; dose response; mortality; squalene; water temperature
Abstract:: ... A formalin-inactivated red sea bream iridovirus (RSIV) vaccine was prepared using the culture supernatant of a persistently infected Pagrus major fin cell line (PI-PMF) with IVS-1 strain (RSIV subtype II Meglaocytivirus). Rock bream (Oplegnathus fasciatus) were injected with a high-dose, ultracentrifuged megalocytivirus vaccine (Ultra HSCMV, 7.0 × 10¹⁰ copies/mL), a high-dose supernatant of cultur ...
DOI:: 10.1016/j.vaccine.2020.11.001
: https://doi.org/10.1016/j.vaccine.2020.11.001

Author:: Zhao Zhao; Yang Li; Guo Chen; Chen Zhang; Gao‐Xue Wang; Bin Zhu
Source:: Aquaculture research 2022 v.53 no.6 pp. 2175-2184
ISSN:: 1355-557X
Subject:: Infectious spleen and kidney necrosis virus; antibodies; antigens; aquaculture; carbon nanotubes; coat proteins; enzymes; fish; host range; immunization; immunogenicity; mannose; mortality; research; subunit vaccines; viruses; China
Abstract:: ... Due to its wide host range and frequently causing significant mortality, infectious spleen and kidney necrosis virus (ISKNV) has caused huge economic losses on mandarin fish in China. There are no commercial drugs to cure ISKNV, and vaccines are regarded as one of the most effective measures to control virus infection. Because of the weak immunogenicity of antigen protein, subunit vaccines may req ...
DOI:: 10.1111/are.15736
: https://doi.org/10.1111/are.15736

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