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1. Alternative Heterologous Expression of L-Arabinose Isomerase from Enterococcus faecium DBFIQ E36 By Residual Whey Lactose Induction

Author:
Ticiane C. de Souza; Ravena Casemiro Oliveira; Saulo Gonçalves Santiago Bezerra; Ricardo M. Manzo; Enrique J. Mammarella; Denise Cavalcante Hissa; Luciana R. B. Gonçalves
Source:
Molecular biotechnology 2021 v.63 no.4 pp. 289-304
ISSN:
1073-6085
Subject:
Enterococcus faecium; affinity chromatography; arabinose; biotechnology; biotransformation; carbon; catalytic activity; enzyme activity; galactose; glucose; glycerol; heterologous gene expression; isomerases; lactose; tagatose; whey
Abstract:
... This study reports an alternative strategy for the expression of a recombinant L-AI from Enterococcus faecium DBFIQ E36 by auto-induction using glucose and glycerol as carbon sources and residual whey lactose as inducer agent. Commercial lactose and isopropyl β-D-1-thiogalactopyranoside (IPTG) were also evaluated as inducers for comparison of enzyme expression levels. The enzymatic extracts were p ...
DOI:
10.1007/s12033-021-00301-2

2. Sequential uptake of aldoses over fructose and enhanced phosphate solubilization in Rhizobium sp. RM

Author:
Akshita Champaneria; Bhagya Iyer; Shalini Rajkumar
Source:
Applied microbiology and biotechnology 2022 v.106 no.11 pp. 4251-4268
ISSN:
0175-7598
Subject:
Rhizobium; aldoses; arabinose; biotechnology; carbon; fructokinase; fructose; gluconates; glucose; malates; metabolism; microbiology; oxalates; oxidation; oxidoreductases; rock phosphate; solubilization; transcription (genetics); tricalcium phosphate; xylose; xylose isomerase
Abstract:
... Rhizobium sp. RM solubilized tri-calcium phosphate (TCP: 324–463 µg ml⁻¹) and rock phosphate (RP: 36–46.58 µg ml⁻¹) in the presence of common rhizospheric sugars-glucose, arabinose, xylose and their combinations. Fructose, though did not support RP solubilization individually, surprisingly solubilized significantly higher phosphate when combined with aldoses. The highest TCP (644 µg ml⁻¹) and RP ( ...
DOI:
10.1007/s00253-022-11997-w

3. Characterization of two extracellular arabinanases in Lactobacillus crispatus

Author:
Qing Li; Michael G. Gänzle
Source:
Applied microbiology and biotechnology 2020 v.104 no.23 pp. 10091-10103
ISSN:
0175-7598
Subject:
Lactobacillus crispatus; acetates; affinity chromatography; amylases; arabinans; arabinose; biotechnology; carbon; fructans; lactic acid; metabolism; operon; pH; pectins; pentose phosphate cycle; phosphoketolase; probiotics; starch; sugar beet; temperature
Abstract:
... Extracellular glycosyl hydrolases are uncommon in lactobacilli and include amylases and fructosidases mediating starch and fructan utilization, respectively. Extracellular arabinanases have not been described in lactobacilli. This study is aimed at identifying the function of an arabinan utilization operon in Lactobacillus crispatus DSM29598 and at characterizing two putative extracellular arabina ...
DOI:
10.1007/s00253-020-10979-0

4. Herbaspirillum seropedicae expresses non-phosphorylative pathways for D-xylose catabolism

Author:
Ana Karen Malán; Thalita Tuleski; Ana Inés Catalán; Emanuel Maltempi de Souza; Silvia Batista
Source:
Applied microbiology and biotechnology 2021 v.105 no.19 pp. 7339-7352
ISSN:
0175-7598
Subject:
Herbaspirillum seropedicae; arabinose; biotechnology; carbon; catabolism; endophytes; genes; hexoses; mutants; oxidoreductases; pentoses; poly-3-hydroxybutyrate; pyruvic acid; sequence analysis; xylose
Abstract:
... Herbaspirillum seropedicae is a β-proteobacterium that establishes as an endophyte in various plants. These bacteria can consume diverse carbon sources, including hexoses and pentoses like D-xylose. D-xylose catabolic pathways have been described in some microorganisms, but databases of genes involved in these routes are limited. This is of special interest in biotechnology, considering that D-xyl ...
DOI:
10.1007/s00253-021-11507-4

5. The potential of extracellular biopolymer production by Mesorhizobium sp. from monosaccharide constituents of lignocellulosic biomass

Author:
Belkis Chalup Silveira Roesler; Renata Gonçalves Vaz; Tereza Cristina Luque Castellane; Eliana Gertrudes de Macedo Lemos; Carlos André Veiga Burkert
Source:
Biotechnology letters 2021 v.43 no.7 pp. 1385-1394
ISSN:
0141-5492
Subject:
Mesorhizobium; arabinose; biomass; biopolymers; biotechnology; carbon; exopolysaccharides; glucose; glucuronic acid; hydrolysates; lignocellulose; rhamnose; soybeans; xylose
Abstract:
... OBJECTIVE: The effects of monosaccharide constituents of lignocellulosic materials on exopolysaccharide (EPS) production by Mesorhizobium sp. Semia 816 were studied. RESULTS: According to the results, by using sugars commonly found in lignocellulosic biomass as carbon sources (glucose, arabinose and xylose), no significant differences were observed in the production of EPS, reaching 3.39 g/L, 3.33 ...
DOI:
10.1007/s10529-021-03119-9

6. High-level production of industrially relevant oxidases by a two-stage fed-batch approach: overcoming catabolite repression in arabinose-inducible Escherichia coli systems

Author:
Ramón Román; Nikola Lončar; Antoni Casablancas; Marco W. Fraaije; Glòria Gonzalez
Source:
Applied microbiology and biotechnology 2020 v.104 no.12 pp. 5337-5345
ISSN:
0175-7598
Subject:
Escherichia coli; arabinose; batch fermentation; biotechnology; carbon; catabolite repression; eugenol; glucose; glycerol; hydroxymethylfurfural; microbiology; oxidoreductases; protein synthesis
Abstract:
... With the growing interest in enzyme applications, there is an urgent demand for economic, affordable, and flexible enzyme production processes. In the present paper, we developed a high cell density fed-batch process for the production of two cofactor-containing oxidase, 5-hydroxymethylfurfural oxidase (HMFO) and eugenol oxidase (EUGO). The approach involved the arabinose-inducible system to drive ...
DOI:
10.1007/s00253-020-10622-y

7. Insights into the cellulose degradation mechanism of the thermophilic fungus Chaetomium thermophilum based on integrated functional omics

Author:
Xin Li; Chao Han; Weiguang Li; Guanjun Chen; Lushan Wang
Source:
Biotechnology for biofuels 2020 v.13 no.1 pp. 143
ISSN:
1754-6834
Subject:
Chaetomium; arabinose; binding capacity; biofuels; biomass; biotechnology; carbon; cellobiose dehydrogenase; cellulose; degradation; design; genes; habitats; industrial applications; lignocellulose; secretion; thermophilic fungi; xylanases
Abstract:
... BACKGROUND: Lignocellulose is the most abundant and renewable biomass resource on the planet. Lignocellulose can be converted into biofuels and high-value compounds; however, its recalcitrance makes its breakdown a challenge. Lytic polysaccharide monooxygenases (LPMOs) offer tremendous promise for the degradation of recalcitrant polysaccharides. Chaetomium thermophilum, having many LPMO-coding gen ...
DOI:
10.1186/s13068-020-01783-z

8. Valorisation of pectin-rich agro-industrial residues by yeasts: potential and challenges

Author:
Luís C. Martins; Catarina C. Monteiro; Paula M. Semedo; Isabel Sá-Correia
Source:
Applied microbiology and biotechnology 2020 v.104 no.15 pp. 6527-6547
ISSN:
0175-7598
Subject:
Saccharomyces cerevisiae; arabinose; biorefining; biotechnology; biotransformation; carbon; catabolism; feedstocks; galacturonic acid; value added; yeasts
Abstract:
... Pectin-rich agro-industrial residues are feedstocks with potential for sustainable biorefineries. They are generated in high amounts worldwide from the industrial processing of fruits and vegetables. The challenges posed to the industrial implementation of efficient bioprocesses are however manyfold and thoroughly discussed in this review paper, mainly at the biological level. The most important y ...
DOI:
10.1007/s00253-020-10697-7

9. Variation in sugarcane biomass composition and enzymatic saccharification of leaves, internodes and roots

Author:
Patrick J. Mason; Agnelo Furtado; Annelie Marquardt; Katrina Hodgson-Kratky; Nam V. Hoang; Frederik C. Botha; Gabriella Papa; Jenny C. Mortimer; Blake Simmons; Robert J. Henry
Source:
Biotechnology for biofuels 2020 v.13 no.1 pp. 201
ISSN:
1754-6834
Subject:
arabinose; biofuels; biomass; biorefining; biotechnology; carbon; cell walls; cellulose; culms; feedstocks; galactose; genotype; hemicellulose; internodes; leaves; lignin; p-coumaric acid; reducing sugars; roots; saccharification; storage; sucrose; sugarcane; tissues
Abstract:
... BACKGROUND: The composition of biomass determines its suitability for different applications within a biorefinery system. The proportion of the major biomass fractions (sugar, cellulose, hemicellulose and lignin) may vary in different sugarcane genotypes and growth environments and different parts of the plant. This study investigated the composition of mature and immature internodes, roots and ma ...
DOI:
10.1186/s13068-020-01837-2

10. Molecular characterization of a glycoside hydrolase family-51 α-L-arabinofuranosidase from Auricularia auricula

Author:
Li Zou; Tingting Sun; Yuanyi Yang; Linfang Zhang; Jian Zhang
Source:
Biotechnology, biotechnological equipment 2017 v.31 no.3 pp. 469-476
ISSN:
1314-3530
Subject:
Auricularia auricula; Escherichia coli; arabinose; biotechnology; carbon; edible fungi; equipment; genes; glucose; glycosides; isoelectric point; maltose; molecular weight; mycelium; polyacrylamide gel electrophoresis; recombinant proteins; sodium dodecyl sulfate; sucrose
Abstract:
... The cDNA encoding α-L-arabinofuranosidase was cloned from the edible fungus Auricularia auricula for the first time. The open reading frame of the α-L-arabinofuranosidase gene abf was 1953 bp encoding 650 amino acids, with a predicted protein molecular weight of 71.19 kDa and a theoretical isoelectric point of 5.23. The putative protein was predicted to belong to the glycoside hydrolase family-51. ...
DOI:
10.1080/13102818.2017.1286233

11. Identification and characterization of d-arabinose reductase and d-arabinose transporters from Pichia stipitis

Author:
Seiya Watanabe; Yuki Utsumi; Shigeki Sawayama; Yasuo Watanabe
Source:
Bioscience, biotechnology, and biochemistry 2016 v.80 no.11 pp. 2151-2158
ISSN:
1347-6947
Subject:
Saccharomyces cerevisiae; Scheffersomyces stipitis; arabinose; biotechnology; carbon; enzyme activity; fungi; glucose; lignocellulose; oxidoreductases; pentoses; xylose
Abstract:
... d-xylose and l-arabinose are the major constituents of plant lignocelluloses, and the related fungal metabolic pathways have been extensively examined. Although Pichia stipitis CBS 6054 grows using d-arabinose as the sole carbon source, the hypothetical pathway has not yet been clarified at the molecular level. We herein purified NAD(P)H-dependent d-arabinose reductase from cells grown on d-arabin ...
DOI:
10.1080/09168451.2016.1204221

12. Beneficial Effect of Corncob Acid Hydrolysate on the Lipid Production by Oleaginous Yeast Trichosporon dermatis

Author:
Lian Xiong; Chao Huang; Xiao-Yan Yang; Xiao-Qing Lin; Xue-Fang Chen; Can Wang; Bo Wang; Xin-An Zeng; Xin-De Chen
Source:
Preparative biochemistry & biotechnology 2015 v.45 no.5 pp. 421-429
ISSN:
1532-2297
Subject:
Trichosporon; acetic acid; arabinose; biomass; biotechnology; carbon; corn cobs; fermentation; glucose; hydrolysates; lignocellulose; lipid content; lipids; xylose; yeasts
Abstract:
... In this work, corncob acid hydrolysate and its simulated medium whose sugar composition was the same as the corncob acid hydrolysate were used as fermentation substrate for lipid production by oleaginous yeast Trichosporon dermatis . On the corncob acid hydrolysate, after 7 days of fermentation, the biomass, lipid content, lipid yield, and lipid coefficient of T. dermatis were 17.3 g/L, 40.2%, 7.0 ...
DOI:
10.1080/10826068.2014.923453

13. Controlled Preparation of Cellulases with Xylanolytic Enzymes from Trichoderma reesei (Hypocrea jecorina) by Continuous-Feed Cultivation Using Soluble Sugars

Author:
Masakazu IKE; Jeung-yil PARK; Mine TABUSE; Ken TOKUYASU
Source:
Bioscience, biotechnology, and biochemistry 2013 v.77 no.1 pp. 161-166
ISSN:
1347-6947
Subject:
Trichoderma reesei; arabinose; biotechnology; biotransformation; carbon; cellobiose; endo-1,4-beta-glucanase; glucose; mutants; xylose
Abstract:
... The objective of this study was to develop an efficient production system for cellulase preparation with a high level of xylanolytic enzymes using soluble carbon sources. When xylose and arabinose were simultaneously fed with glucose and cellobiose, a mutant of Trichoderma reesei, M3-1, showed sufficient levels of cellulolytic and xylanolytic activities, indicating that xylose and arabinose are go ...
DOI:
10.1271/bbb.120696

14. Exopolysaccharides, proteins and lipids in Pleurotus pulmonarius submerged culture using different carbon sources

Author:
F.R. Smiderle; L.M. Olsen; A.C. Ruthes; P.A. Czelusniak; A.P. Santana-Filho; G.L. Sassaki; P.A.J. Gorin; M. Iacomini
Source:
Carbohydrate polymers 2012 v.87 no.1 pp. 368-376
ISSN:
0144-8617
Subject:
Pleurotus pulmonarius; arabinose; biomass; biotechnology; carbon; culture media; essential amino acids; exopolysaccharides; galactose; glucans; glucose; medicinal properties; mushroom growing; mushrooms; mycelium; nutrient content; nutritive value; proteins; unsaturated fatty acids; xylose
Abstract:
... For many years mushrooms have been consumed and appreciated by their nutritional value, and medicinal properties. The traditional mushroom cultivation takes too long and the macrofungi biotechnology has not been explored in its full potential yet. The goal of this work was to observe if different carbon sources could improve the yield and diversify fungi nutrient composition in submerged culture. ...
DOI:
10.1016/j.carbpol.2011.07.063

15. Production of the amino acids l-glutamate, l-lysine, l-ornithine and l-arginine from arabinose by recombinant Corynebacterium glutamicum

Author:
Jens Schneider; Karin Niermann; Volker F. Wendisch
Source:
Journal of biotechnology 2011 v.154 no.2-3 pp. 191-198
ISSN:
0168-1656
Subject:
Corynebacterium glutamicum; Escherichia coli; arabinose; bacteria; biotechnology; carbon; fructose; glucose; glutamic acid; hydrolysates; hydrolysis; lysine; molasses; operon; starch; sucrose
Abstract:
... Amino acid production processes with Corynebacterium glutamicum are based on media containing glucose from starch hydrolysis or fructose and sucrose as present in molasses. Simultaneous utilization of various carbon sources, including glucose, fructose and sucrose, in blends is a typical characteristic of this bacterium. The renewable non-food carbon source arabinose, which is present in hemicellu ...
DOI:
10.1016/j.jbiotec.2010.07.009

16. Effect of Carbon Sources on the Induction of Xylanolytic-Cellulolytic Multienzyme Complexes in Paenibacillus curdlanolyticus Strain B-6

Author:
Rattiya WAEONUKUL; Khin Lay KYU; Kazuo SAKKA; Khanok RATANAKHANOKCHAI
Source:
Bioscience, biotechnology, and biochemistry 2008 v.72 no.2 pp. 321-328
ISSN:
1347-6947
Subject:
Paenibacillus curdlanolyticus; arabinose; bacteria; biotechnology; carbon; cellobiose; cellulose; corn husks; galactose; glucose; polyacrylamide gel electrophoresis; polymers; sugarcane bagasse; xylan; xylose
Abstract:
... The effect of polymeric substances such as α-cellulose, birchwood xylan, corn hull, and sugarcane bagasse, and of soluble sugars such as L-arabinose, D-galactose, D-glucose, D-xylose, and cellobiose, on the induction of multienzyme complexes in a facultatively anaerobic bacterium, Paenibacillus curdlanolyticus B-6, was investigated under aerobic conditions. Cells and culture supernatants of strain ...
DOI:
10.1271/bbb.70333