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... An investigation was conducted to determine the nature of genetic effects in segregating populations produced by crossing selected progenitor species-derived tobacco (Nicotiana tabacum L.) lines to the tobacco cultivar used in the original interspecific hybridization. Two genetically stable tobacco lines developed from interspecific crosses of N. tabacum ✕ N. sylvestris Speg. and Comes and N. taba ...
... Total alkaloids (predominately nicotine) are important constitutents in flue-cured tobacco (Nicotiana tabacum L.). Due to varying nicotine levels in cigarettes, it is of interest to see what effect varying alkaloid levels in tobacco have on certain agronomic and chemical characteristics of the cured leaf. Experiments were conducted at two locations to develop lines of flue-cured tobacco with diffe ...
... The reproductive isolation barriers and the mating patterns among Pinus pumila, P. parviflora var. pentaphylla and their hybrids were examined by flowering phenology and genetic assays of three life stages: airborne-pollen grains, adults and seeds, in a hybrid zone on Mount Apoi, Hokkaido, Japan. Chloroplast DNA composition of the airborne-pollen was determined by single-pollen polymerase chain re ...
... Fusion experiments were performed with a first (BC1-6738) and a second (BC2-9017) generation backcross hybrid of 6x Solanum nigrum (+) 2x potato somatic hybrids with potato cultivars. Because no progeny was obtained from the BC2 genotypes, alternative approaches were sought to overcome the sexual crossing barrier. Five potato genotypes, one of which contains the hygromycin resistance gene, were us ...
... The cotton (Gossypium hirsutum L.) collection of primitive accessions is underutilized for cotton improvement because they are photoperiodic. Day-neutrality can be achieved by crossing with a day-neutral cultivar and selection in the F2 generation. Our objective was to determine the relationship among four backcross generations and parents using amplified fragment length polymorphism (AFLP) marker ...
... Drought stress is one of the major constraints affecting rice production and yield stability in the rainfed regions. To understand the physiological basis of drought resistance related component traits, we used a backcross inbred population of rice under three kinds of moisture regimes viz., non-stress, moderate (24.48%) and severe stress (73.97%) conditions which reflect the differential response ...
... The Australian fresh market pineapple breeding program commenced in 1991 with the objective of developing clones more suited to fresh consumption than the industry standard clone, 'Smooth Cayenne'. These early crosses developed approximately 60,000 seedlings from which the 2 cultivars 'Aus-Jubilee' and 'Aus-Festival' have been commercialised. Plant Breeders Rights have been used to protect intelle ...
... The genetic base of commercial cucumber (Cucumis sativus L.) is extremely narrow (about 3%–8% polymorphism). Wide-based crosses within C. sativus [i.e., C. sativus var. sativus x C. sativus var. hardwickii (R.) Alef.] and interspecific hybridization attempts before 1995 have not substantially increased genetic diversity for plant improvement. However, in 1995, an amphidiploid (Cucumis hytivus Chen ...
... Numerous Brassica napus (+) Arabidopsis thaliana somatic hybrids were screened for male sterility and aberrant flower phenotypes. Nine hybrids were selected and backcrossed recurrently to B. napus. The resulting lines displayed stable maternal inheritance of flower phenotypes. Nuclear and organellar genomes were characterized molecularly using RFLP analysis. No DNA from A. thaliana was found in th ...
... Apospory in Pennisetum squamulatum Fresen is inherited as a single-locus dominant trait. Molecular analysis has identified the apospory-specific genomic region (ASGR), a large segment of one chromosome that is necessary and sufficient to confer apomixis. To transfer apospory from P. squamulatum to pearl millet [P. glaucum (L.) R. Br.], advanced backcrosses were generated. Here we report the charac ...
... Several sets of wheat inter-varietal chromosome substitution lines (SLs) have been produced over the last fifty years at the CRI (formerly RICP) in Prague-Ruzyně, based on cytogenetic manipulations using aneuploids. Lines with defined genes have been obtained which significantly influence growth habit and flowering time and these have been used particularly in the study of the genetics and physiol ...
Steven R. Larson; Xiaolei Wu; Thomas A. Jones; Kevin B. Jensen; N. Jerry Chatterton; Blair L. Waldron; Joseph G. Robins; B. Shaun Bushman; Antonio J. Palazzo
... Leymus cinereus (Scribn. & Merr.) Á. Löve and L. triticoides (Buckley) Pilg. are tall caespitose and short rhizomatous perennial Triticeae grasses, respectively. Circumference of rhizome spreading, proportion of bolting culms, anthesis date, and plant height were evaluated in two mapping families derived from two interspecific hybrids of L. cinereus Acc:636 and L. triticoides Acc:641 accessions, b ...
... BACKGROUND: Primula is a large genus of flowering herbs well known for their heterostyly. Currently few natural hybrids are known and reproductive barriers in this genus in the wild have received little attention. However, there is instance of hybridization between rare and widely-spread species, and conservation implications of such situation is poorly understood. In the present study, we investi ...
... The present study examined the occurrence of open and controlled pollination between Pinus brutia (Ten.) and Pinus halepensis (Mill.) under the environmental conditions of the East Mediterranean climate. Controlled interspecific pollination succeeded only with P. brutia as the maternal parent and P. halepensis as the paternal parent. Open interspecific pollination also occurred under Israeli condi ...
... In many crops, unharvested seeds can create populations of volunteer plants that increase opportunities for crop-to-wild gene flow. Pollen-mediated gene flow between cultivated and wild sunflower (both Helianthus annuus, Asteraceae) is well documented, but the role of seed dispersal and volunteers has not been investigated. We compared flowering times and other phenotypic traits of volunteers from ...
... Maples are common street and shade trees throughout the temperate zone. They are widely used for their wide range of ornamental traits and adaptability, particularly to urban settings. Unfortunately, some species such as Acer tataricum ssp. ginnala (Amur maple) and A. platanoides (Norway maple) have escaped cultivation to become pests or in some cases threaten native flora. However, these species ...
... Fire blight, caused by Erwinia amylovora, is a major concern in apple production since no existing control option has been proven to be completely effective and durable. The use of resistant apple cultivars in apple production could and should be an importance piece of an integrated management of the disease. High levels of resistance to Erwinia amylovora have been found in wild or ornamental appl ...
... Zea mays ssp. parviglumis is the progenitor of maize and assume to have tolerance against various biotic and abiotic stresses. It grows without human intervention in Southern Mexico at the bank of Balsas river and maintains sufficient genetic diversity that can help in maize diversification and domestication of wild alleles. To develop the uniform near to homozygous line the maize inbred parent DI ...
... Previous locations of flowering time (FT) QTL in several Brassica species, coupled with Arabidopsis synteny, suggest that orthologues of the genes FLC, FY or CONSTANS might be the candidates. We focused on FLC, and cloned paralogous copies in Brassica oleracea, obtained their genomic DNA sequences, and confirmed their locations relative to those of known FT-QTL by genetical mapping. They varied in ...
... The persistence and stability of a transgene encoding a Bacillus thuringiensis (Bt) Cry1Ac insecticidal protein was investigated in hybrids between crop Brassica napus and a recurrent wild Brassica juncea population. Interspecific hybrids (F1) and backcross progenies (BC1, BC2) containing green fluorescent protein (GFP) and Bt genes were successfully produced in the greenhouse. Stable Bt toxin lev ...
... To study the cytoplasmic effects on heat tolerance, the nuclear genomes of ten alloplasmic lines were substituted by four or five backcrosses using ‘Karl 92’, ‘Ventnor’, ‘U1275’ and ‘Jagger’ as the recurrent parent. During the final backcross, reciprocal crosses were made to develop cytoplasmic near isogenic lines. Sixty-eight BC5F1/BC4F1 lines and their parents were evaluated in growth chambers f ...
... The genome of bread wheat, AABBDD, was substituted into the cytoplasm of Hordeum chilense by repeated backcrossing to produce alloplasmic lines. The aim of this work was to investigate the effect of H. chilense cytoplasm on agronomic traits in common wheat. Three cytolines were developed. The alloplasmic nature of these lines was confirmed using chloroplast simple-sequence repeat markers. Each cyt ...
... Upland cotton (Gossypium hirsutum) is the most important fiber crop and an important oilseed crop in the world. Developing drought tolerant cultivars is important to sustainable cotton production in arid and semi-arid regions. However, there is a lack of information on genetic variation for drought tolerance in breeding populations. In this study, a backcross inbred line (BIL) Upland population wi ...
... The short-day plant rice varies greatly in photoperiod sensitivity (PS) for flowering. The hybrid F₁ from a cross between the day-neutral pure line EM93-1 and the weedy rice accession SS18-2 had stronger PS than SS18-2. Some BC₁ (EM93-1/F₁) segregates were even more sensitive to photoperiod than the F₁, as indicated by later flowering or no flowering after 250 days under a 14-h long daylength. A g ...
... Lilium lancifolium Thunb. exhibits wide genetic diversity and numerous genetic traits within progeny populations. Parent morphology affects the distribution and assortment of progeny phenotypes. In this study, morphological analysis and fluorescence in situ hybridization (FISH) were conducted for cross progeny (F1 and BC1). Results showed that F1-1 hybrids had greater plant height than the parents ...
... Yield losses of maize due to drought stress (DS) in environments with limited rains can reach 100 %. Introgression of favorable alleles from diverse landraces into adapted elite varieties will improve performance, hence reducing losses. The objective of this study was to assess the performance of BC populations under DS and multiple locations (ML) after introgression of alleles from diverse maize ...
... The expression and heritability of quantitative traits were examined in four hybrid mungbean populations, developed by crossing two cultivars, Kiloga and Berken, with two Australian wild accessions, ACC 1 and ACC 87. Phenological, morphological, agronomic and pod and seed traits were measured in the parental, F1, F2, and two F1×parent backcross progeny generations. Plants were grown in large pots ...
... Bacterial grain rot (BGR), caused by the bacterial pathogen Burkholderia glumae, is a destructive disease of rice. At anthesis, rice panicles are attacked by the pathogen, and the infection causes unfilled or aborted grains, reducing grain yield and quality. Thus, increasing the level of BGR resistance is an important objective for rice breeding. A quantitative trait locus (QTL) on rice chromosome ...
... KEY MESSAGE : This study fine mapped and validated a QTL on rice chromosome 4 that increases spikelet fertility under high temperature (over 37 °C) at the flowering stage. Climate change has a negative effect on crop production and food security. Understanding the genetic mechanism of heat tolerance and developing heat-tolerant varieties is essential to cope with future global warming. Previously, ...
Oryza sativa; backcrossing; early development; field experimentation; flowering; inbred lines; loci; models; photoperiod; quantitative trait loci; rice; temperature
Abstract:
... In this study we have attempted to quantify the thermal and photoperiodical responses of rice (Oryza sativa L.) flowering time QTLs jointly by a ‘date-of-planting’ field experiment of a mapping population, and a ‘phenological model’ analysis that separately parameterizes the two responses, based on daily temperature, daily photoperiod and flowering date. For this purpose, the ‘three-stage Beta mod ...
... A double mutant with streaked leaf and abnormal floret was found and temporarily named streaked leaf and floral organ number mutant (st-fon). For this mutant, besides white streak appeared on culm, leaves and panicles, the number of floral organs increased and florets cracked. The extreme phenotype was that several small florets grew from one floret or branch rachis in small florets extended and d ...
... Male sterility enabled commercialization of heterosis in rice but low seed set remains a constraint on hybrid dissemination. We evaluated 216 F6 maintainer lines for agronomic and floral characteristics in augmented design and selected 15 maintainer lines, which were testcrossed with IR58025A. Five backcrosses were conducted to transfer cytoplasmic male sterility (CMS) to select maintai ...
... Background Collections of nearly isogenic lines where each line carries a delimited portion of a donor source genome into a common recipient genetic background are known as introgression libraries and have already shown to be instrumental for the dissection of quantitative traits. By means of marker-assisted backcrossing, we have produced an introgression library using the extremely early-flowerin ...
Capsicum annuum; backcrossing; cluster analysis; cultivars; flowering; fruiting; fruits; genetic variation; hot peppers; ornamental plants; pepper; seeds; Brazil
Abstract:
... The genus Capsicum has been used as an ornamental plant, but there are only a few commercial cultivars for this purpose available in Brazil. The aim of this study was to analyze the genetic diversity in a structured family of six generations of ornamental pepper based on quantitative and qualitative data, separately and integrated in the same analysis. These studies are part of the Capsicum breedi ...
... Striga is an important parasitic weed causing substantial economic losses in cereal and legume crop production in sub-Saharan Africa. Integrated Striga management approaches such as a combined use of Striga resistant varieties and Fusarium oxysporum f.sp. strigae (FOS), a biocontrol agent of Striga, are an option to control the parasite and to boost sorghum productivity. Understanding host gene ac ...
... • Different tomato (Solanum lycopersicum) mutants, affected in flowering time, reproductive structure or plant architecture, were crossed to produce double mutants in order to investigate gene interactions in flowering regulation in this autonomous species with a sympodial growth habit. • The compound inflorescence: uniflora, uniflora: self pruning, uniflora: blind, and jointless: uniflora double ...
... Development and evaluation of introgression lines have become one of the promising approaches to understanding genetic mechanisms of important traits in crops. In this study, three sets of drought tolerance (DT) selected backcross populations in maize, including two BC₂S₁ populations and one BC₃S₁ population, from a cross of Qi319 and Huangzaosi (recurrent parent) were developed by selecting for s ...
Prunus yedoensis; backcrossing; cherries; flowering; genes; genetic markers; genomics; genotype; hybrid species; introgression; nucleotide sequences; phylogeny; population structure; progeny; South Korea
Abstract:
... Prunus yedoensis Matsumura is one of the popular ornamental flowering cherry trees native to northeastern Asia, and its wild populations have only been found on Jeju Island, Korea. Previous studies suggested that wild P. yedoensis (P. yedoensis var. nudiflora) is a hybrid species; however, there is no solid evidence on its exact parental origin and genomic organization. In this study, we developed ...
... Exotic germplasm is often used in maize (Zea mays L.) breeding programs. However, the occurrence of genotype × environment interactions (GEIs) may mask the potential utility of exotic material. Our objective was to understand better GEIs for temperate, temperate × highland, and highland tropical maize genotypes cultivated under temperate or highland tropical conditions. We related yield instabilit ...
... Understanding response of plants to drought stress is critical for developing drought tolerant genotypes with stable performance under water limiting conditions. A backcross inbred population derived in the background of a popular variety Swarna, was evaluated for yield and yield attributing traits under reproductive stage and shoot morphological traits at seedling stage under drought stress over ...
... Hybridization among plant species may have considerable evolutionary consequences, from the formation of new taxa to the extinction of species by genetic swamping. The evolutionary fate of a system of hybridizing species is highly dependent on fitness differences between parental and hybrid genotypes. In an introgressed hybrid swarm of the rare Senecio hercynicus and its widely distributed congene ...
... Fusarium verticillioides (Sacc.) Nirenberg (synonym F. moniliforme Sheldon) (teleomorph: Gibberella moniliformis) and F. proliferatum (Matsushima) Nirenberg (teleomorph: G. intermedia) are fungal pathogens of maize (Zea mays L.) that cause ear rot and contaminate grain with fumonisins, a family of mycotoxins that adversely affect animal and human health. The objective of this study was to estimate ...
... Numerous quantitative trait loci (QTLs) have been identified from segregating hybrid populations between Upland (Gossypium hirsutum) and Pima (G. barbadense) cotton. However, the genetic basis of these QTLs is currently unknown. In the present study, an interspecific backcross inbred line (BIL) population was tested at two locations in 2005 and 2006, and used to identify and isolate polymorphic tr ...
... Drought is a major limiting factor for barley production, especially in the primary areas of its cultivation. Wild barley represents a major source of favourable alleles for increasing the genetic variation for multiple traits including resistance to both biotic and abiotic stresses. We used advanced backcross quantitative trait locus (AB-QTL) analysis of a BC3-doubled haploid population developed ...
... Shifts in weather patterns and increases in the frequency and magnitude of extreme weather events resulting from unfavorable climate change have led to frequent flooding and brown planthopper outbreaks in rice fields, particularly those in rainfed lowland areas in Thailand. The traditional jasmine rice cultivar KDML105, which is popularly grown in rainfed lowland areas in north and northeast Thail ...
... Virus-resistant transgenic squash are grown throughout the United States and much of Mexico and it is likely that the virus-resistant transgene (VRT) has been introduced to wild populations repeatedly. The evolutionary fate of any resistance gene in wild populations and its environmental impacts depend upon trade-offs between the costs and benefits of the resistance gene. In a 3-year field study u ...
... The productivity of cowpea (Vigna unguiculata (L.) Walp.] can be reduced substantially by hot weather during flowering. Inheritance and heritability of tolerance to heat during reproductive development were studied to develop efficient methods for breeding improved cultivars. Heat tolerant (‘Prima’ and TVu 4552) and heat sensitive (Barnbey 23, ‘Magnolia’ and 7964) cowpea genotypes were chosen for ...
... In globe artichoke (Cynara cadunculus var. scolymus), the presence of entire leaf type in adult plants is a characteristic of the early Catanesi group, as 'Violet de Provence' and 'Blanca de Tudela', among others. Both of these attributes (i.e., entire leaf and precocity) failed to be transmitted by sexual means. In the cultivated cardoon (Cynara cadunculus var. altilis) some materials also presen ...