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Lachnospiraceae; Salmonella Typhimurium; analytical chemistry; bacteria; biosensors; electrochemistry; electrodes; electron transfer; hybridization chain reaction; oligonucleotides; polymers; single-stranded DNA
Abstract:
... In this study, Lba Cas12a (Cpf1) as one of the CRISPR systems from Lachnospiraceae bacterium was coupled with a hybridization chain reaction (HCR) to develop an electrochemical biosensor for detecting the pathogenic bacterium, Salmonella typhimurium. Autonomous cross-opening of functional DNA hairpin structures of HCR yielded polymer double-stranded DNA wires consisting of numerous single-stranded ...
... Pulsed ohmic heating (POH) is a promising alternative to conventional heat processing for microbial safety. It has been reported that the efficacy of POH depends on the concentration of electrolytes. This research investigated the effect of sodium lactate as an electrolyte on the efficacy of POH with respect to the heating rate and inactivation of pathogens in PBS and soybean milk. When PBS and so ...
Rhodopseudomonas palustris; ambient temperature; bacteria; biosynthesis; carbon dioxide; electrodes; electron transfer; electron transport chain; electrosynthesis; energy; greenhouse gases; photosynthetic reaction centers; proteomics; ubiquinones; value added
Abstract:
... Microbial electrosynthesis can convert greenhouse gas CO₂ into value-added chemicals at room temperature and mild conditions, and the efficiency of its reaction is closely dependent on the electrode potential and electron transport chain-related functional proteins. In this study, the photosynthetic bacterium Rhodopseudomonas palustris CGA009 was used to explore CO₂ bioreduction behavior at differ ...
... Biofouling of membranes in water treatment is considered as one of the major practical problems. A novel and an efficient approach for cleaning biofilm grown on the membrane surface is proposed by applying a direct electric current (124 mA, 90 s) through platinum electrodes inside a cross-flow ultrafiltration channel. Depending on the electrochemical reactions occurring at the electrodes, either c ...
... Surface-enhanced Raman spectroscopy (SERS) is a vibrational method successfully applied in analytical chemistry, molecular biology and medical diagnostics. In this article, we demonstrate the combination of the negative dielectrophoretic (nDEP) phenomenon and a flexible surface-enhanced Raman platform for quick isolation (3 min), concentration and label-free identification of bacteria. The platfor ...
Mateusz Brodowski; Marcin Kowalski; Marta Skwarecka; Katarzyna Pałka; Michał Skowicki; Anna Kula; Tomasz Lipiński; Anna Dettlaff; Mateusz Ficek; Jacek Ryl; Karolina Dziąbowska; Dawid Nidzworski; Robert Bogdanowicz
... This study reports a novel impedimetric immunosensor for protein D detection in purified and bacterial (Haemophilus influenzae, Hi) samples. The detection was based on antigen recognition by anti-protein D antibodies (apD) immobilised at the maze-like boron-doped carbon nanowall electrodes (B:CNW). The B:CNW electrodes were synthesised, and their surface was characterised by scanning electron micr ...
... The reduction of Fe(III) by metal-reducing bacteria through extracellular electron transfer (EET) is a critical link in the biogeochemical cycle of As/Fe, and humic substances are believed to play a role in this process. In this study, the indigenous As-resistant bacterium Bacillus D2201 isolated from the Datong Basin was responsible for the valence transition of Fe and As in the groundwater envir ...
... Electro-fermentation (EF) is an upcoming technology that can control the metabolism of exoelectrogenic bacteria (i.e., bacteria that transfer electrons using an extracellular mechanism). The fermenter consists of electrodes that act as sink and source for the production and movement of electrons and protons, thus generating electricity and producing valuable products. The conventional process of f ...
... Nisin is an antimicrobial peptide with bacterial, fungicidal, virucidal properties, attacking bacteria and destroying the cell membranes. Thanks to its stability to hard conditions, it is a candidate for the use as molecular recognition elements in biosensing platform. In this work, the use of nisin as a biological molecule for the development of a sensitive biosensor for bacteria detection is rep ...
... A sufficiently fast and simple antimicrobial susceptibility testing (AST) is urgently required to guide effective antibiotic usages and to surveil the antimicrobial resistance rate. Here, we establish a rapid, quantitative, and high-throughput phenotypic AST by measuring electrons transferred from the interiors of microbial cells to external electrodes. Because the transferred electrons are based ...
ammonium nitrogen; antibiotic resistance genes; bacteria; bacterial communities; chemical oxygen demand; constructed wetlands; electrodes; fuels; ibuprofen; metagenomics; microbial fuel cells; personal care products; prediction; sulfadiazine; toxicity; wastewater
Abstract:
... Microbial fuel cell constructed wetlands (CW-MFCs) with different circuit operation conditions and hydraulic retention time (HRT) were constructed to evaluate their ability to remove and accumulate pharmaceutical and personal care products (PPCPs) (sulfadiazine (SDZ), carbamazepine (CBZ), naproxen (NPX) and ibuprofen (IBP)) during four months running process. The abundance level of corresponding s ...
... Screening of pathogenic bacteria plays a crucial role in preventing foodborne disease outbreaks. In this study, an ultrasensitive biosensor was developed for fast detection of Salmonella using self-assembled magnetic nanoparticle (MNP) chains for continuous-flow separation of Salmonella from large-volume sample, urease coated gold nanoparticles (GNPs) for specific labelling of Salmonella and effic ...
... Natural biomass is a promising candidate for the preparation of heteroatom-doped carbons that can be used as effective electrode catalysts for microbial fuel cells (MFCs), due to its intriguing features, such as high abundance, rich heteroatom contents, and low cost. In this review, we summarize recent process in the design and fabrication of MFC electrodes based on biocarbons; discuss the effects ...
Bacillus subtilis; Escherichia coli; Pantoea agglomerans; Pseudomonas putida; Salmonella Enteritidis; Staphylococcus aureus; analytical chemistry; antibodies; bacteria; electrical conductivity; electrochemistry; electrodes; endo-1,4-beta-glucanase; human health; magnetism; milk; oligonucleotides; tap water
Abstract:
... Pathogen-associated infections represent one of the major threats to human health and require reliable methods for immediate and robust identification of pathogenic microorganisms. Here, an inexpensive cellulase-linked immunomagnetic methodology was developed for the specific and ultrasensitive analysis of bacteria at their single-cell levels within a 3 h procedure. Detection of a model bacterium, ...
bacteria; biodegradation; bioremediation; electric field; electrodes; equations; ions; petroleum; saline soils; soil water; soil water retention; synergism; water content; water supply
Abstract:
... This study investigated changes in soil ion content and soil water-holding capacity during electro-bioremediation (EK-Bio) of petroleum contaminated saline soil (ion content of 3.92 g/kg). The results indicated that the soil ions surrounded the electrodes with increasing time, thus changing the soil water-holding capacity. According to the Van Genuchten model fitting results, the soil residual wat ...
Actinobacteria; bacteria; bioremediation; community structure; degradation; electric field; electric potential difference; electrodes; fungi; microbial biomass; microbial communities; phospholipid fatty acids; pollutants; polluted soils; polycyclic aromatic hydrocarbons; soil pH; technology; temperature; water content
Abstract:
... Electro-bioremediation is a promising technology for remediation of soil contaminated with persistent organic compounds such as polycyclic aromatic hydrocarbons (PAHs). During electro-bioremediation, electrical fields have been shown to increase pollutant degradation. However, it remains unclear whether there is an optimal strength for the electrical field applied that is conductive to the maximum ...
... Shewanella oneidensis MR-1, a model species of exoelectrogenic bacteria (EEB), has been widely applied in bioelectrochemical systems. Biofilms of EEB grown on electrodes are essential in governing the current output and power density of bioelectrochemical systems. The MR-1 genome is exceptionally dynamic due to the existence of a large number of insertion sequence (IS) elements. However, to date, ...
... By combining a functionalized microwire sensor with a dielectrophoresis (DEP) technique, a rapid and accurate detection method for bacterial pathogens was developed and demonstrated. The gold-tungsten microsensor (sensing diameter, 25 μm) was functionalized with specific Escherichia coli (E. coli) antibodies against surface antigens to capture E. coli bacterial cells from phosphate buffer saline ( ...
... This study proposes a microfluidic device capable of separating monocytes from a type of cancer cell that is called T-cell acute lymphoblastic leukemia (RPMI-8402) in a continuous flow using negative and positive dielectrophoretic forces. The use of both the hydrodynamic and dielectrophoretic forces allows the separation of RPMI-8402 from monocytes based on differences in their intrinsic electrica ...
... Due to long-chain fatty acids (LCFAs) and acidification, rapeseed oil as a typical lipid in kitchen waste is difficult to be biodegraded by anaerobic digestion. It has been reported that incorporation of some conductive materials into reactors treating complex organic matter could enhance reactor performance. In this study, the aim was to study this possibility of application of granular activated ...
Staphylococcus epidermidis; antibiotic resistance; antibiotics; bacteria; bacterial adhesion; bacterial infections; biofilm; biosensors; electrodes; films (materials); gentian violet; health care workers; magnesium; medical treatment; nanomaterials; transistors; zinc oxide
Abstract:
... Early stage detection of biofilm formation is an important aspect of microbial research because once formed, biofilms show serious tolerance to antibiotics in contrast to the free-floating bacteria, which significantly increases the difficulty for clinical treatment of bacterial infections. The early stage detection technology is desired to improve the efficiency of medical treatments. In this wor ...
... The early detection of bacteria is of critical importance in addressing serious public health problems. Here, an electrochemical biosensor for rapid detection of bacteria based on facile synthesis of silver wire across electrodes was constructed. High-variable region of 16S rRNA of bacteria was used as biomarker. Polymerase-free synthesis of silver wire was introduced into electrochemical signal t ...
... The conductive polyurethane/polypyrrole/graphene (CPU/PPy/Gr) particle electrode was prepared by an in-situ oxidative polymerization method and used as particle electrodes to degrade levofloxacin (LEV) in a three-dimensional electrode reactor. The prepared CPU/PPy/Gr electrode was characterized systematically and the effects of initial pH, initial LEV concentration, aeration volume, voltage, and e ...
... Salmonella contamination of various seasonings and spices has occurred in the last decade. Radio frequency (RF) heating has been shown to be a potential alternative inactivation method for pathogens in low moisture foods (LMF), including spices and vegetable powders. This study aimed to (1) determine the thermal resistance (D and z values) of Salmonella and Enterococcus faecium(E. faecium) NRRL B- ...
Geobacter sulfurreducens; acetates; bacteria; cytochromes; data collection; electrodes; electron transfer; energy metabolism; fimbriae; gene expression regulation; genes; iron; mutants; palladium; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; structural proteins; transcription (genetics); transcriptomics
Abstract:
... Geobacter sulfurreducens is capable of reducing Pd(II) to Pd(0) using acetate as electron donor; however, the biochemical and genetic mechanisms involved in this process have not been described. In this work, we carried out transcriptome profiling analysis to identify the genes involved in Pd(II) reduction in this bacterium. Our results showed that 252 genes were upregulated while 141 were downreg ...
... There is an urgent need to develop in situ sensors that monitor the continued release of H₂S from biological systems to understand H₂S-related pathology and pharmacology. For this purpose, we have developed a molybdenum disulfide supported double-layered zinc cobaltite modified carbon cloth electrode (MoS₂–ZnCo₂O₄–ZnCo₂O₄) based electrocatalytic sensor. The results of our study suggest that the Mo ...
... OBJECTIVE: To effectively and conveniently detect pathogenic bacteria, this study aimed to develop label-free biosensors fabricated affinity peptides that can recognize targeted bacteria strains and enable precise quantitative detections. RESULTS: A 12-mer peptide with high binding affinity toward Escherichia coli O157:H7 was discovered by biopanning of phage-displayed peptide library. The peptide ...
... Antibiotics are considered emerging pollutants which indiscriminate use has led to the development of antibiotic-resistant bacteria, while their improper disposal has caused adverse effects to the environment and human health. Thus, the development of devices or techniques capable of detecting antibiotics with high sensitivity, low detection limits, and reasonable cost becomes of prime importance. ...
... Electrogenic bacteria metabolize organic substrates by transferring electrons to the external electrode, with subsequent electricity generation. In this proof-of-concept study, we present a novel strain of a known, electrogenic Arcobacter butzleri that can grow primarily on acetate and lactate and its electric current density is positively correlated (R² = 0.95) to the COD concentrations up to 200 ...
... Once pathogens form a biofilm, they become more tolerant to drugs and quicker to recover from physical removal than planktonic cells. Because such robustness of a biofilm is associated with the active metabolism of its constituent microbes, establishment of a direct assay quantifying biofilm's metabolic activity is important for developing antibiofilm substrates and techniques. Current production ...
... It is the ambition of many researchers to finally be able to close in on the fundamental, coupled phenomena that occur during the formation and expression of electrocatalytic activity in electroactive biofilms. It is because of this desire to understand that bioelectrochemical systems (BESs) have been miniaturized into microBES by taking advantage of the worldwide development of microfluidics. Mic ...
... Herein, the mutual effect between azo dye and the performance of electrochemically active bacteria (EAB) is investigated in detail, which is crucial to understand and control the bio-electrochemical systems (BESs) operation for azo dye containing wastewater treatment. EAB is enriched at controlled potential of −0.2 V vs Ag/AgCl in single-chamber BESs. Over 95% azo dye (alizarin yellow R (AYR)) was ...
... In this work, the denitrification of nitrate-contaminated groundwater by electrocoagulation (EC) method using aluminum electrodes has been studied for the production of drinking water. Tests were carried out on four samples collected from the Casablanca-Settat region. EC was investigated for a fixed applied voltage (30 V), the submerged surface area of each electrode plate (33.75 cm²) and the inte ...
... An electrochemical dynamic membrane filtration (EDMF) system for simultaneous solid-liquid separation (also protecting electrodes against fouling) and sewage disinfection was developed. At a low voltage of 2.5 V, efficient disinfection performance was achieved in the EDMF, with ~100% log removal efficiency (no detectable bacteria in the effluent). Results also demonstrated that the EDMF system, op ...
Escherichia coli; absorbance; bacteria; cuprous oxide; electric current; electrochemistry; electrodes; electrolytes; hydrogen peroxide; irradiation; lamps; light; molybdenum disulfide; oxidation; photochemistry; pollutants; sodium chloride; solar collectors; solar radiation; superoxide anion; titanium dioxide; wastewater; water treatment
Abstract:
... Here we present photoelectrochemical (PEC) bacterial inactivation properties of large−scale fabricable Ti/MoS₂/MoOₓ photoanode with a strong solar light absorbance capacity. Specifically, by thermal oxidation of the as−prepared MoS₂/Ti film at 250 °C for 15 min in aerobic condition, the visible light performance of photocurrent generation and Escherichia coli (E. coli) inactivation are markedly en ...
... The bacterium Kluyvera georgiana MCC 3673 transfers electrons directly to the electrode for bio-electricity generation in microbial fuel cell (MFC). This could be due to the formation of biofilm on the surface of electrode or with through the extracellular appendages, or both. The role of extracellular appendages pili and flagella in exo-electron transfer mechanism was investigated. The expression ...
Geobacter sulfurreducens; active sites; bacteria; catalysts; catalytic activity; cost effectiveness; electrochemistry; electrodes; green chemistry; hydrogen; iron; manganese; oxidation; oxygen production; phosphorus; photosystem II; renewable energy sources; surface area
Abstract:
... The development of a low cost and efficient oxygen evolution reaction (OER) catalyst has paramount importance to meet the future sustainable energy demand. Nature's photosynthetic machinery deploy manganese-based complex in the photosystem II to oxidize water. Inspired by nature, herein, we synthesized a high performing manganese-based OER catalyst using an electrochemically active and iron-rich b ...
... A growing number of bacteria are recognized to conduct electrons across their cell envelope, and yet molecular details of the mechanisms supporting this process remain unknown. Here, we report the atomic structure of an outer membrane spanning protein complex, MtrAB, that is representative of a protein family known to transport electrons between the interior and exterior environments of phylogenet ...
Geobacter sulfurreducens; atomic force microscopy; bacteria; cell death; cytochromes; electrodes; electrolytes; electron transfer; fimbriae; polymers
Abstract:
... In this work, we aim to provide a better understanding of the reasons behind electron transfer inefficiencies between electrogenic bacteria and the electrode in microbial fuel cells. We do so using a self-doped conjugated polyelectrolyte (CPE) as the electrode surface, onto which Geobacter sulfurreducens is placed, then using conductive atomic force microscopy (C-AFM) to directly visualize and qua ...
bacteria; biosensors; cadmium; copper; cosmetics; electrodes; electron transfer; ferrocenes; graphene; heavy metals; lead; median effective concentration; monitoring; p-nitrophenol; perfumes; phenol; screening; toxic substances; toxicity; zinc
Abstract:
... A mediator biosensor based on Paracoccus yeei bacteria for assaying the toxicity of perfumery and cosmetics samples was developed. An approach to selecting an electron-transport mediator based on the heterogeneous electron transfer constants for investigated mediators (ks) and the mediator–biomaterial interaction constants (kinteract) was proposed. Screening of nine compounds as potential mediator ...
... Novel CuInS₂/m-BiVO₄ composites with different mass ratios (CuInS₂ to m-BiVO₄ = 1:3, 1:1, and 3:1) were synthesized and their potential visible-light photocatalytic applications for photodegradation of some organic dyes (methylene blue, rhodamine B, and methyl orange) and inactivation of bacteria (Pseudomonas aeruginosa) were investigated. CuInS₂/m-BiVO₄ with a mass ratio of 1:3 exhibited better p ...
Giamwemberg A. Barreto; Marta S. P. Carepo; Ana C. S. Gondim; Wellinson G. Guimarães; Luiz G. F. Lopes; Paul V. Bernhardt; Tércio F. Paulo; Eduardo H. S. Sousa; Izaura C. N. Diógenes
... Tuberculosis is one of the oldest known infectious diseases, responsible for millions of deaths annually around the world. The ability of Mycobacterium tuberculosis (Mtb) to enter into a dormant state has been considered integral to the success of this bacterium as a human pathogen. One of the key systems involved in regulating the entrance into dormancy is the differentially expressed in virulent ...
... The early detection of bacterium plays a significant role in addressing serious public health issues. In this paper, a supersensitive multichannel series piezoelectric quartz crystal (MSPQC) sensor of bacterium based on 16S rRNA and “DNA-RNA switch” was constructed. The fragment in specific region of 16S rRNA was used as the biomarker of bacterium to ensure high specificity and to achieve the accu ...
... A square-planar Cu(II) complex, [Cu(Me-Im)(gly-gly)]∙H2O 1 (Me-Im = 1-methyl-imidazole, gly-gly = glycylglycinato), has been prepared and structurally characterized by single crystal X-ray. The complex 1 was tested for its ability to the transfer RNA by UV–vis spectroscopy, cyclic voltammetry (CV), capillary electrophoresis (CE). Comparative spectroscopic analysis shows a maximum fluorescence-quen ...
... This paper reports the design, fabrication and testing of a microfluidic based impedance biosensor for rapid and simultaneous detection of three Salmonella serogroups. The microfluidic device consists of three microchannels, each one includes a region for focusing the Salmonella cells into the centerline of the microchannel and direct them toward the sensing region to obtain highly concentrated sa ...
Firmicutes; anaerobic conditions; bacteria; catalytic activity; cecum; electrodes; electron transfer; genes; guinea pigs; human health; intestinal microorganisms; mice; physiology; rats
Abstract:
... As a well-studied biochemical reduction process in environmental microbiology, extracellular electron transfer (EET) was recently discovered in bacteria closely related to human health, and orthologues of a flavin-based EET gene were found in the genomes of many species across Firmicutes, a major phylum in mammalian gut microbiota. However, EET has not yet been confirmed to occur in mammalian gut, ...
Acidithiobacillus ferrooxidans; bacteria; biocatalysts; carbon; carbon nanofibers; cytochrome c; cytochrome-c oxidase; electrochemistry; electrodes; electron transfer; electron transport chain; hydrophobicity; oxygen; pH
Abstract:
... Cytochrome c oxidases (CcOs) are the terminal enzymes in energy-converting chains of microorganisms, where they reduce oxygen into water. Their affinity for O₂ makes them attractive biocatalysts for technological devices in which O₂ concentration is limited, but the high overpotentials they display on electrodes severely limit their applicative use. Here, the CcO of the acidophilic bacterium Acidi ...
... Nanochannels hold great prospects in intelligent systems; however, current research focuses on the inner space of the nanochannel while the outer surface is rarely explored. Here, we report on a cooperation mode of the outer surface and inner space of the nanochannel using an integrated nanochannel-electrode (INCE) and its application as a separation–detection system for rapid and facile detection ...
adsorption; aerobic conditions; bacteria; carboxymethylcellulose; color; electric field; electrodes; quaternary ammonium compounds; transmission electron microscopy; zeta potential
Abstract:
... Nanofibrillated bacterial cellulose (NFBC) is produced by culturing a cellulose-producing bacterium under agitated aerobic conditions in a carboxymethylcellulose (CMC)-supplemented medium. Detailed structural analyses revealed that NFBC fiber widths varied with the degree of substitution of the CMC used, and zeta potential values decreased with the increment of CMC concentration in the medium. Tra ...
... Because of numerous merits (e.g., the possibility of their synthesis in 1-D, 2-D, and 3-D forms, large surface-to-volume ratio, and flexible framework functionality), metal-organic frameworks (MOFs) are envisaged as excellent media for the development of biosensors for diverse analytes present in environmental media. The present research work, for the first time, reports the development of a Cu-MO ...
... The composition of the ship's ballast water is complex and contains a large number of microalgae cells, bacteria, microplastics, and other microparticles. To increase the accuracy and efficiency of detection of the microalgae cells in ballast water, a new microfluidic chip for continuous separation of microalgae cells based on alternating current dielectrophoresis was proposed. In this microfluidi ...
Geobacillus; bacteria; biofilm; cell walls; electrochemistry; electrodes; electron transfer; electrons; flavins; high performance liquid chromatography; matrix-assisted laser desorption-ionization mass spectrometry; minerals; moieties; polysaccharides; synthetic products
Abstract:
... Through extracellular electron transfer (EET), bacteria are capable of transforming different insoluble materials of geochemical interest into energy-rich molecules for their growth. For this process, bacteria have been depending directly or indirectly on molecules synthesized within the cells or by various synthetics as mediators. Herein, we studied the in situ change in electrochemistry and supp ...
... In this study, the effects of indirect voltage supply to an anaerobic digestion (AD) reactor on methane production and the removal of chemical oxygen demand (COD) were studied at different organic loading rates (OLRs) of food waste by the circulation from an auxiliary bio-electrochemical reactor (ABER) with stainless steel (STS304) electrodes. The effects of the indirect voltage on microbial commu ...
... The degradation of polychlorinated biphenyls (PCBs) and total organic carbon (TOC) by sediment microbial fuel cell (SMFC) with/without nanoscale zero-valent iron (NZVI) addition was investigated. It was found that the combined application led to the highest removal efficiencies of PCBs (37.55 ± 1.11%) and TOC (49.72 ± 1.54%) in all circumstances and produced a higher power density (108.89 mW/m2) a ...
bacteria; byproducts; cell walls; chlorine; disinfection; electric current; electrodes; electrolysis; pH; scanning electron microscopes; seawater; temperature
Abstract:
... Conventional water disinfection processes suffer from several disadvantages including safety and handling issues and may result in the formation of undesirable and often harmful by-products. Novel disinfection methods, such as the application of low electric current, may present more practical, viable, and sustainable processes. The present study investigates the effects of applying a low-intensit ...
... This study evaluated the anti-biofouling efficacy of capacitor mode and resistor mode in membrane distillation (MD). Polytetrafluoroethylene (PTFE) membrane coated with carbon nanotube (CNT) was adopted as the electrically conductive membrane. The biofouling formation on the pre-treatment membrane was systematically analyzed, and the results showed that both operation modes had obvious inhabitatio ...
... Constructed wetlands (CWs) performance enhancement can be done with intensification strategies. A recent strategy still in study is the coupling with Microbial Electrochemical Technologies (MET). An alternative system using electro-conductive biofilters instead of electrodes and circuits used in MET, resulted in the development of a Microbial Electrochemical-based CW (METland). This system relies ...
... Some of microorganisms are potential pathogens that can be infectious agents under some circumstances, and development of new detection methods of the pathogens is of high interest. In the present study, an Enterococcus faecalis (E. faecalis) DNA biosensor (ef-biosensor) was fabricated to quantify the bacterium genome. A specific E. faecalis DNA probe was selected from 16S rDNA sequence of E. faec ...
... This work reports on further development of an inhibition electrochemical sensor array based on immobilized bacteria for the preliminary detection of a wide range of organic and inorganic pollutants, such as heavy metal salts (HgCl₂, PbCl₂, CdCl₂), pesticides (atrazine, simazine, DDVP), and petrochemicals (hexane, octane, pentane, toluene, pyrene, and ethanol) in water. A series of DC and AC elect ...
Protozoa; algae; bacteria; biomass production; electric potential difference; electrodes; pH; ponds; predators; salinity; temperature
Abstract:
... Large scale cultivation of algae is becoming a major challenge due to the impact of biotic (grazers and predators) and abiotic (salinity, light, temperature and pH) stress factors on growth and biomass productivity. Historically, to control grazers and predators, various physical, chemical and biological methods have been used which have had detrimental effects on algae growth and environment. Con ...
... The extracellular electron transfer of Shewanella oneidensis MR-1 (MR-1) has been extensively studied due to the importance of the biosensors and energy applications of bioelectrochemical systems. However, the oxidation of metal compounds by MR-1, which represents the inward extracellular electron transfer from extracellular electron donors into the microbe, is barely understood. In this study, MR ...
... Boron-doped diamond electrodes can be used to generate reactive oxygen species directly at the electrode’s surface. This property was used in this study for in-situ electrochemical oxidation to eliminate different bacteria, i.e. Escherichia coli, Pseudomonas fluorescens and Pseudomonas aeruginosa, as well as Bacillus subtilis spores from water samples. Application of low voltages in the rage from ...
... Triphenyl phosphate (TPHP) is one of the major organophosphate esters (OPEs) with increasing consumption. Considering its largely distribution and high toxicity in aquatic environment, it is important to explore an efficient treatment for TPHP. This study aimed to investigate the accelerated degradation of TPHP in a three-electrode single chamber bioelectrochemical system (BES). Significant increa ...
... Bacterial infections are causing worldwide morbidity and mortality. One way to limit infectious outbreaks and optimize clinical management of infections is through the development of fast and sensitive sensing of bacteria. Most sensing approaches are currently based on immunological detection principles. We report here on an impedimetric sensor to selectively and sensitive detect uropathogenic E. ...
... Mycobacterium leprae causes endemic disease leprosy which becomes chronic if not treated timely. To expedite this ‘timely diagnosis’, and that also at an early stage, here an attempt is made to fabricate an epitope-imprinted sensor. A molecularly imprinted polymer nanoparticles modified electrochemical quartz crystal microbalance sensor was developed for sensing of Mycobacterium leprae bacteria th ...
... Bioelectrochemical systems (BESs) are hybrid systems using electroactive bacteria and solid electrodes, which serve as electron donor or acceptor for microorganisms. When forming a biofilm on the electrode, bacteria secrete extracellular polymeric substances (EPSs). However, EPS excretion of electroactive biofilms in BES has been rarely studied so far. Consequently, the aim of this study is to dev ...
... In biosensors fabrication, entrapment in polymeric matrices allows efficient immobilization of the biorecognition elements without compromising their structure and activity. When considering living cells, the biocompatibility of both the matrix and the polymerization procedure are additional critical factors. Bio-polymeric gels (e.g. alginate) are biocompatible and polymerize under mild conditions ...
acetates; activated carbon; bacteria; biofilm; carbon dioxide; electrochemistry; electrodes; electron transfer; electrosynthesis; iron oxides; microbial activity; microbial communities; renewable energy sources; value-added products
Abstract:
... Microbial electrosynthesis (MES) allows the transformation of CO₂ into value-added products by coupling with renewable energy. The enhancement in the microbial activity and electron transfer rate via a new electrode modification method is essential for developing MES. Here, three groups of granular activated carbon decorated by Fe₃O₄ (Fe₃O₄/GAC) with mass fractions of 23%, 38% and 50% were prepare ...
bacteria; bacterial culture; capacitance; carbon; carbonization; electrochemical capacitors; electrodes; electrolytes; energy; energy density; ionic liquids; lignin; micropores; pollution; surface area
Abstract:
... The conversion of low-cost renewable lignin could enable the economic fabrication of carbon materials for energy storage devices. However, the traditional activation methods for the production of a lignin-derived porous carbon with a large specific surface area are complex and expensive, and some activators can cause pollution. In this paper, we propose a novel green bacterial activation method fo ...
Tomáš Mackuľak; Roman Grabic; Viera Špalková; Noemi Belišová; Andrea Škulcová; Ondřej Slavík; Pavel Horký; Miroslav Gál; Jan Filip; Ján Híveš; Marian Vojs; Andrea Vojs Staňová; Alžbeta Medveďová; Marián Marton; Lucia Birošová
antibiotic resistance; antibiotics; bacteria; cotinine; electrodes; hospitals; illicit drugs; metabolites; oxidation; pollutants; sanitation; sulfamethoxazole; tandem mass spectrometry; wastewater; Slovakia
Abstract:
... Various types of micropollutants, e.g., pharmaceuticals and their metabolites and resistant strains of pathogenic microorganisms, are usually found in hospital wastewaters. The aim of this paper was to study the presence of 74 frequently used pharmaceuticals, legal and illegal drugs, and antibiotic-resistant bacteria in 5 hospital wastewaters in Slovakia and Czechia and to compare the efficiency o ...
... Biomineralization-associated proteins are responsible for the structure-forming process of biominerals. Aiming to regulate the synthesis of nanostructured TiO₂ anatase, an artificial protein 5R5 derived from silaffins was displayed on Escherichia coli surface through genetic manipulation. The genetically modified bacterial cells serve as the framework for the formation of rod-shaped TiO₂, assemble ...
... The biofilm mode of growth protects bacterial cells from applied disinfection methods for abiotic (food) contact surfaces. Therefore, new inactivation technologies such as Cold Atmospheric Plasma (CAP) should be considered. However, the influence of different plasma characteristics on the CAP efficacy for biofilm inactivation requires further study. In this research, the influence of (i) the appli ...
Romana Jarošová; Sandra E. Mcclure; Margaret Gajda; Milica Jović; Hubert H. Girault; Andreas Lesch; Michael Maiden; Christopher Waters; Greg M. Swain
... Polyacrylamide-coated, carbon nanotube (PA/CNT) electrodes were prepared by an inkjet printing process and used to measure pyocyanin and uric acid in a wound fluid simulant at 37 °C. These two molecules are potential indicators of infection, and therefore their detection could prove useful for monitoring wound healing. Pyocyanin is a marker for the common wound bacterium Pseudomonas aeruginosa. Ou ...
Acinetobacter calcoaceticus; Sinorhizobium; Sphingobacterium multivorum; ambient temperature; bacteria; carboxylic ester hydrolases; diesel fuel; electric potential difference; electrodes; genes; hydrocarbons; models; petroleum; polluted soils; soil pH; solar collectors; solar energy; sustainable technology; water content; winter
Abstract:
... This study investigated the isolation and characterization of three novel bacterial strains; Acinetobacter calcoaceticus, Sphingobacterium multivorum, and Sinorhizobium, isolated form agriculture land. From three hundred strains of bacteria, the three isolates were identified for their superior diesel degradation ability by a series of bench-scale tests. The isolates were further investigated in b ...
... We assessed the efficacy of bacterial inactivation using a dielectric barrier discharge in three different plasma setups: plasma gas (PG), and direct and indirect plasma activated water (PAW), where deionized water was placed either between or away from the electrodes, respectively. We used bioluminescent Escherichia coli K12 lux as a model bacteria in a biosensor format to study the inactivation ...
... A MEMS‐based impedance biosensor was designed, fabricated, and tested to effectively detect the presence of bacterial cells including E. coli O157:H7 and Salmonella typhimurium in raw chicken products using detection region made of multiple interdigitated electrode arrays. A positive dielectrophoresis based focusing electrode was used in order to focus and concentrate the bacterial cells at the ce ...
Acinetobacter calcoaceticus; Sinorhizobium; Sphingobacterium multivorum; ambient temperature; bacteria; bioremediation; diesel fuel; electric potential difference; electrodes; polluted soils; sand; soil pH; solar collectors; solar energy; sustainable technology; water content; winter
Abstract:
... This study investigated the effectiveness of bioelectrokinetics in rehabilitating a silty clayey sand contaminated with diesel fuel using three novel bacterial strains; Acinetobacter calcoaceticus, Sphingobacterium multivorum, and Sinorhizobium, isolated form agriculture land. Three electrokinetic bioremediation cells were used to conduct the tests and a novel electrode configuration technique was ...
... We demonstrate a method of concentrating and patterning of biological cells on a chip, exploiting the confluence of electric and thermal fields, without necessitating the use of any external heating or illuminating sources. The technique simply employs two parallel plate electrodes and an insulating layer over the bottom electrode, with a drilled insulating layer for inducing localized variations ...
Staphylococcus epidermidis; bacteria; disinfection; drinking water; electric field; electric potential difference; electrodes; electroporation; fluorescence microscopy; glass; lab-on-a-chip technology; models; platinum; pulsed electric fields; staining
Abstract:
... Electroporation based locally enhanced electric field treatment (LEEFT) is an emerging bacteria inactivation technology for drinking water disinfection. Nevertheless, the lethal electroporation threshold (LET) for bacteria has not been studied, partly due to the tedious work required by traditional experimental methods. Here, a lab-on-a-chip device composed of platinum electrodes deposited on a gl ...
... Though well known for its anti-microbial property, copper is usually not considered for drinking water disinfection because of its health risk to human bodies under efficient biocidal concentration. Herein, we have rationally designed and constructed a tubular coaxial-electrode copper ionization cell (CECIC) that enables superior disinfection performance (~6-log removal of E. coli) with a very low ...
... BACKGROUND: Antibiotics and antibiotic resistance genes (ARGs) are two pollutants in soil, especially ARGs as one of the top three threats to human health. The performance of soil microbial fuel cells (MFCs) fuelled with antibiotics was investigated. RESULTS: In this study, soil MFCs spiked with tetracycline exhibited optimal bioelectricity generation, which was 25% and 733% higher than those of M ...
... The traditional bacterial identification method of growing colonies on agar plates can take several days to weeks to complete depending on the growth rate of the bacteria. Successfully decreasing this analysis time requires cell isolation followed by identification. One way to decrease analysis time is by combining dielectrophoresis (DEP), a common technique used for cell sorting and isolation, an ...
... Finding fast and reliable ways to detect pathogenic bacteria is crucial for addressing serious public health issues in clinical, environmental, and food settings. Here, we present a novel assay based on the conversion of an electrochemical signal into a more convenient optical readout for the visual detection of Escherichia coli. Electropolymerizing polyaniline (PANI) on an indium tin oxide screen ...
activated sludge; anaerobic digestion; bacteria; biogas; carbon dioxide; chemical oxygen demand; electrochemistry; electrodes; energy; hydrolysis; land application; mass transfer; methane; organic matter; solubilization; wastewater treatment
Abstract:
... Sludge generated after wastewater treatment represents an important challenge due to the large amounts produced and the need to adequately treat it. Anaerobic digestion is the preferred treatment process to obtain renewable energy as well as a biosolid with the potential to be reused in land application. This process generates biogas (methane and carbon dioxide) that may be used for energy co-gene ...
... Rapidly evolving cold atmospheric pressure plasma (CAPP)–based technology has been actively used not only in bioresearch but also in biotechnology, food safety and processing, agriculture, and medicine. High variability in plasma device configurations and electrode layouts has accelerated non-thermal plasma applications in treatment of various biomaterials and surfaces of all sizes. Mode of cold p ...
... Owing to the ability for efficient electric interaction with electrodes, electrochemically active bacteria (EAB) attract considerable attention in biotechnology. These bacteria are involved not only in the generation of electricity from organic wastes in microbial fuel cells but also in the production of valued chemicals with the aid of electric energy in microbial electrosynthesis systems. It has ...
... Bacterial synthesis of polyhydroxybutyrates (PHBs) is a potential approach for producing biodegradable plastics. This study assessed the ability of Rhodopseudomonas palustris TIE-1 to produce PHBs under various conditions. We focused on photoautotrophy using a poised electrode (photoelectroautotrophy) or ferrous iron (photoferroautotrophy) as electron donors. Growth conditions were tested with eit ...
... Traditional microbiology analysis is usually hindered by the long time-cost and lack of portability in many urgent situations. In this work, we developed a novel electrochemical DNA biosensor (E-biosensor) for sensitive analysis of the 16S rRNA gene of five bacteria, using a consecutive adenine (polyA) probe. The polyA probe consists of a polyA tail and a recognition part. The polyA tail can combi ...
... Bacterial infections present one of the leading causes of mortality worldwide, resulting in an urgent need for sensitive, selective, cost-efficient, and easy-to-handle technologies to rapidly detect contaminations and infections with pathogens. The presented research reports a fully functional chemical-detection principle, addressing all of the above-mentioned requirements for a successful biosens ...
... Electrode modifications with conductive and nanostructured polyaniline (PANI) were recognized as efficient approach to improve interaction between electrode surface and electrogenic bacteria for boosting the performance of microbial fuel cell (MFC). However, it still showed undesirable performance because of the challenge to control the orientation (such as vertical alignment) of PANI nanostructur ...
... Geobacter bacteria assemble a helical peptide of the Type IVa pilin subclass as conductive pili decorated with metal binding and reduction sites. We used recombinant techniques to synthesize thiolated pilin derivatives and self-assembled them on gold electrodes as a monolayer that concentrated the metal traps at the liquid interface. Cyclic and step potential voltammetry demonstrated the conductiv ...
bacteria; byproducts; carcinogenicity; chlorination; copper; copper nanoparticles; disinfection; electric potential difference; electrodes; electroporation; energy efficiency; humans; morbidity; mortality; nanowires; ozonation; pathogens; purification methods; regrowth; ultraviolet radiation; water purification
Abstract:
... Pathogen infection has become the major reason for human morbidity and mortality in the world. However, common bacterial inactivation methods (e.g., chlorination, ultraviolet radiation, and ozonization) have significant drawbacks such as carcinogenic byproduct formation, energy intensiveness, and/or regrowth of pathogens. Nanowire-assisted low-voltage electroporation enables effective and energy-e ...
... The aim of the present study was to isolate an indigenous acidophilic bacterium from tannery effluent contaminated sludge (TECS) sample and evaluate its potentiality towards the removal of zinc using an integrated approach of bioleaching enhanced electrokinetic remediation (BEER) technology in zinc spiked soil at an initial concentration of 1000 mg/kg. The isolated acidophilic bacterium was charac ...
... BACKGROUND: The microbial electrolysis cell (MEC) has been widely reported as an efficient strategy to enhance anaerobic digestion. However, the role of the MEC during acidogenesis for treatment of sulfate‐containing wastes remains unclear. In this study a pair of electrodes was placed into an acidogenic reactor to form MEC‐based acidogenesis to investigate its performance in sulfate‐containing wa ...
... Turning wastewater directly into electricity is alluring, widespread use of microbial fuel cells (MFCs) to achieve this at industrial scale appears increasingly unlikely despite intense research efforts lasting over a decade. Such endeavors have not been futile, however, and game-changing discoveries have resulted from these well-intentioned, scientifically rigorous but ultimately frustrated attem ...
Md Rakibul Hasan; Thiruchelvi Pulingam; Jimmy Nelson Appaturi; Anis Nadyra Zifruddin; Swe Jyan Teh; Teck Wei Lim; Fatimah Ibrahim; Bey Fen Leo; Kwai Lin Thong
... In this study, an amino-modified aptasensor using multi-walled carbon nanotubes (MWCNTs)-deposited ITO electrode was prepared and evaluated for the detection of pathogenic Salmonella bacteria. An amino-modified aptamer (ssDNA) which binds selectively to whole-cell Salmonella was immobilised on the COOH-rich MWCNTs to produce the ssDNA/MWCNT/ITO electrode. The morphology of the MWCNT before and aft ...
... An electrochemical platform was designed using biocompatible quaternary ammonium salts containing alkyl groups with different chain lengths as electrode materials for visible protein immobilization on a glassy carbon (GC) electrode. The electrode was constructed using a simple self-assembly method relying on the electrostatic interaction between negatively charged hemoglobin (Hb) and positively ch ...
... This is a first review paper that delineates fundamental disinfection mechanism undergoes during the simple electrochemical coagulation (EC) process. The elucidation of detailed mechanistic phenomenon of EC process involved would help to enhance the disinfection efficiency. In this context, the biomass (bacteria, virus and algae) abatement mechanism by EC is critically reviewed and rationalized ba ...
... We have developed innovative assays that can detect enzymes rapidly. Paracetamol- or catechol-bearing compounds, when exposed to their respective enzymes, released paracetamol or catechol, which can be detected using a standard glucose meter. This approach was used to detect a number of diverse analytes that include enzymes such as β-galactosidase and α-mannosidase and pathogens such as influenza ...
... The increasing lack of drinking water around the globe is of great concern. Although UV irradiation, photocatalysis, and electrocatalysis for bacterial disinfection have been widely explored, the synergistic kinetics involved in these strategies have not been reported to date. Herein, we report on an efficient and cost-effective strategy for the remediation of a model bacterium (E. coli), through ...