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You searched for: Subject beta-glucuronidase Remove constraint Subject: beta-glucuronidase Subject Helianthus annuus Remove constraint Subject: Helianthus annuus Subject term Helianthus annuus Remove constraint Subject term: Helianthus annuus
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1. Transformation of sunflower (Helianthus annuus L.) following wounding with glass beads

Author:
Grayburn, W.S.; Vick, B.A.
Source:
Plant cell reports 1995 v.14 no.5 pp. 285-289
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; Helianthus annuus; seedlings; Agrobacterium radiobacter; cotyledons; varieties; gene expression; beta-glucuronidase
Abstract:
... A procedure was developed for transformation of Helianthus annuus (sunflower) using Agrobacterium tumefaciens. Cotyledons were removed from young seedlings, and the remaining tissue was uniformly wounded by shaking with glass beads. The wounded tissue was then co-cultivated with a hypervirulent strain of Agrobacterium tumefaciens harboring the binary plasmid pCNL56. Minimal use of defined medium w ...
DOI:
10.1007/BF00232029
PubMed:
24186761

2. Gene transfer by particle bombardment to Norway spruce and Scots pine pollen

Author:
Haggman, H.M.; Aronen, T.S.; Nikkanen, T.O.
Source:
Canadian journal of forest research = 1997 v.27 no.6 pp. 928-935
ISSN:
0045-5067
Subject:
Pinus sylvestris; Picea abies; transgenic plants; pollen; beta-glucuronidase; gene expression; Helianthus annuus; biolistics; pollination; genetic transformation; promoter regions
Abstract:
... The aim of the present work was to genetically transform Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies (L.) Karst.) pollen in order to study the possibilities of obtaining transgenic progenies. The effects of different pollen origins and regulatory sequences on the beta-glucuronidase (GUS) expression in pollen were significant. The highest GUS expression in pine (55 +/- 7.7% of g ...
DOI:
10.1139/x97-024

3. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

4. Dual regulation of a heat shock promoter during embryogenesis: stage-dependent role of heat shock elements

Author:
Almoguera, C.; Prieto-Dapens, P.; Jordano, J.
Source:
plant journal 1998 v.13 no.4 pp. 437-446
ISSN:
0960-7412
Subject:
heat shock proteins; plant development; Helianthus annuus; seeds; transgenic plants; enzyme activity; mutation; gene expression; histochemistry; Nicotiana tabacum; reporter genes; binding sites; embryogenesis; promoter regions; beta-glucuronidase
Abstract:
... Transgenic tobacco expression was analysed of chimeric genes with point mutations in the heat shock element (HSE) arrays of small heat shock protein (sHSP) gene from sunflower hp17.7 G4. The promotor was developmentally regulated during zygotic and responded to heat stress in vegetative tissues. Mutations in the HSE affected nucleotides crucial for human heat shock transcription factor 1 (HSF1) bi ...
DOI:
10.1046/j.1365-313X.1998.00044.x
PubMed:
9680992

5. Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.): a simple protocol

Author:
Sankara Rao, K.; Rohini, V.K.
Source:
Annals of botany 1999 v.83 no.4 pp. 347-354
ISSN:
0305-7364
Subject:
genetic transformation; Helianthus annuus; seedlings; Agrobacterium radiobacter; transgenic plants; cotyledons; gene expression; rooting; reporter genes; inheritance (genetics); phosphotransferases (kinases); enzyme activity; culture media; beta-glucuronidase; shoots
Abstract:
... A simple and reproducible Agrobacterium tumefaciens-mediated transformation system was developed for sunflower (Helianthus annuus L.) 'KBSH- l'. The objective was to substantially eliminate the in vitro regeneration component from the transformation protocol. Two-d-old seedlings with one cotyledon detached were infected with Agrobacterium tumefaciens strain LBA 4404/pKIWI105 harbouring genes for b ...
DOI:
10.1006/anbo.1998.0828

6. Reversible heat-induced inactivation of chimeric beta-glucuronidase in transgenic plants

Author:
Almoguera, C.; Rojas, A.; Jordano, J.
Source:
Plant physiology 2002 v.129 no.1 pp. 333-341
ISSN:
0032-0889
Subject:
Helianthus annuus; Nicotiana tabacum; tobacco; transgenic plants; seedlings; seeds; plant proteins; heat shock proteins; recombinant proteins; gene expression; messenger RNA; biosynthesis; heat stress; beta-glucuronidase; genetic markers; protein products; enzyme activity; temperature; plant extracts; amino acid sequences; nucleotide sequences; chemical constituents of plants; transcription (genetics); stress response
Abstract:
... We compared the expression patterns in transgenic tobacco (Nicotiana tabacum) of two chimeric genes: a translational fusion to beta-glucuronidase (GUS) and a transcriptional fusion, both with the same promoter and 5'-flanking sequences of Ha hsp17.7 G4, a small heat shock protein (sHSP) gene from sunflower (Helianthus annuus). We found that immediately after heat shock, the induced expression from ...
PubMed:
12011363
PubMed Central:
PMC155896

7. Induction of the Hahsp17.7G4 promoter by root-knot nematodes: involvement of heat-shock elements in promoter activity in giant cells

Author:
Escobar, C.; Barcala, M.; Portillo, M.; Almoguera, C.; Jordano, J.; Fenoll, C.
Source:
Molecular plant-microbe interactions 2003 v.16 no.12 pp. 1062-1068
ISSN:
0894-0282
Subject:
Nicotiana tabacum; tobacco; transgenic plants; Helianthus annuus; promoter regions; heat shock proteins; recombinant DNA; reporter genes; beta-glucuronidase; gene induction; Meloidogyne incognita; root-knot nematodes; giant cells; root galls; roots; point mutation
Abstract:
... Root-knot nematodes feed from specialized giant cells induced in the plants that they parasitize. We found that the promoter of the Hahsp17.7G4 gene, which encodes a small heat-shock protein involved in embryogenesis and stress responses, directed GUS expression in tobacco galls induced by the root-knot nematode Meloidogyne incognita. In roots containing a GUS reporter fusion to the Hahsp17.7G4 pr ...
DOI:
10.1094/MPMI.2003.16.12.1062
PubMed:
14651339

8. Stable genetic transformation of high oleic Helianthus annuus L. genotypes with high efficiency

Author:
Mohamed, S.; Boehm, R.; Schnabl, H.
Source:
Plant science 2006 v.171 no.5 pp. 546-554
ISSN:
0168-9452
Subject:
Helianthus annuus; oil crops; genetic variation; transgenic plants; shoot meristems; genetic transformation; beta-glucuronidase; transgenes; reporter genes; gene expression regulation; inheritance (genetics); green fluorescent protein
DOI:
10.1016/j.plantsci.2006.05.012

9. The sunflower HD-Zip transcription factor HAHB4 is up-regulated in darkness, reducing the transcription of photosynthesis-related genes

Author:
Manavella, Pablo A.; Dezar, Carlos A.; Ariel, Federico D.; Drincovich, María F.; Chan, Raquel L.
Source:
Journal of experimental botany 2008 v.59 no.11 pp. 3143-3155
ISSN:
0022-0957
Subject:
Arabidopsis thaliana; transcription factors; messenger RNA; Helianthus annuus; complementary DNA; transgenic plants; drought tolerance; gene expression regulation; transcription (genetics); gene expression; response elements; light; reporter genes; electrophoresis; chlorophyll; promoter regions; beta-glucuronidase; leaves; pigments
Abstract:
... HAHB4 belongs to the sunflower subfamily I of HD-Zip proteins and is involved in drought-tolerance response and ethylene-mediated senescence. Cross-talk between these two processes through this transcription factor was recently described. In this study it is shown that the expression of HAHB4 is induced in darkness and quickly disappears when plants are exposed to light. This regulation of HAHB4 w ...
DOI:
10.1093/jxb/ern170
PubMed:
18603614

10. Agrobacterium-mediated transformation of cotyledons of mature seeds of multiple genotypes of sunflower (Helianthus annuus L.)

Author:
M. Sujatha; S. Vijay; S. Vasavi; P. Veera Reddy; S. Chander Rao
Source:
Plant cell, tissue, and organ culture 2012 v.110 no.2 pp. 275-287
ISSN:
0167-6857
Subject:
monogenic inheritance; Helianthus annuus; enzymatic treatment; plasmids; hybrids; kanamycin kinase; Agrobacterium radiobacter; ultrasonic treatment; gene expression; genotype; restorer lines; in vitro regeneration; oilseed crops; genetic transformation; kanamycin; adventitious shoots; transgenic plants; cotyledons; reporter genes; beta-glucuronidase; acetosyringone
Abstract:
... Lack of a genotype-independent transformation protocol for sunflower is a major bottleneck in improving this important oilseed crop. An efficient Agrobacterium-mediated transformation protocol is described, which was adaptable across a broad range of sunflower genotypes. The improved transformation approach used cotyledons from mature seeds vertically split through the embryo axis. The LBA 4404 Ag ...
DOI:
10.1007/s11240-012-0149-2