U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject beta-glucuronidase Remove constraint Subject: beta-glucuronidase Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum Subject Brassica napus Remove constraint Subject: Brassica napus Subject term Brassica napus Remove constraint Subject term: Brassica napus Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
Start Over

Search

3  Search Results

1 - 3 of 3
Number of results to display per page

Search Results

1. Cell specific, cross-species expression of myrosinases in Brassica napus, Arabidopsis thaliana and Nicotiana tabacum

Author:
Thangstad, O.P.; Gilde, B.; Chadchawan, S.; Seem, M.; Husebye, H.; Bradley, D.; Bones, A.M.
Source:
Plant molecular biology 2004 v.54 no.4 pp. 597-611
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; guard cells; recombinant DNA; transgenic plants; embryo (plant); gene expression; phloem; Nicotiana tabacum; Brassica napus; thioglucosidase; reporter genes; tobacco; enzyme activity; promoter regions; beta-glucuronidase; rapeseed
Abstract:
... A prototypical characteristic of the Brassicaceae is the presence of the myrosinase-glucosinolate system. Myrosinase, the only known S-glycosidase in plants, degrades glucosinolates, thereby initiating the formation of isothiocyanates, nitriles and other reactive products with biological activities. We have used myrosinase gene promoters from Brassica napus and Arabidopsis thaliana fused to the β- ...
PubMed:
15316292

2. Analyses in transgenic tobacco of the promoter from a Brassica napus gene highly expressed in the stigma

Author:
Robert, L.S.; Levesque-Lemay, M.; Gerster, J.L.; Hong, H.P.; Keller, W.
Source:
Plant cell reports 1999 v.18 no.5 pp. 357-362
ISSN:
0721-7714
Subject:
Arabidopsis thaliana; genetic transformation; gene transfer; transgenic plants; Agrobacterium radiobacter; developmental stages; clones; gene expression; stigma; amino acid sequences; Nicotiana tabacum; genes; Brassica napus; nucleotide sequences; enzyme activity; histochemistry; beta-glucuronidase
Abstract:
... A genomic clone, Pis G363, containing the Brassica napus stigma-expressed gene Pis 63-2 was isolated and sequenced. The coding region of Pis G363 does not possess introns and shows 82% identity to the nucleotide sequence of a gene from Arabidopsis BAC clone T01B08. A 2-kb promoter fragment from Pis G363 was fused to the coding sequence of the marker enzyme beta-glucuronidase (GUS) and introduced i ...
DOI:
10.1007/s002990050586

3. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...