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You searched for: Subject beta-glucuronidase Remove constraint Subject: beta-glucuronidase Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum
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1. Optimizing the particle bombardment conditions in cell suspension cultures of Nicotiana tabacum and expression of the recombinant antihypertensive amarantin

Author:
D.U. Santos-Ballardo; L.J. Germán-Báez; D.L. Ambriz-Pérez; K.A. Meza-Ayala; K.D. Luna-Avelar; A. Valdez-Ortiz
Source:
South African journal of botany 2019 v.125 pp. 329-336
ISSN:
0254-6299
Subject:
Nicotiana tabacum; Western blotting; beta-glucuronidase; biolistics; callus; cell suspension culture; helium; histology; osmotic treatment; polymerase chain reaction; recombinant proteins; reporter genes; sorbitol; statistical analysis; tobacco; transgenes; transgenic plants
Abstract:
... The first successful attempt to produce transgenic tobacco cell suspensions expressing a modified version of amarantin, through direct gene transfer using particle gun is described. The physical parameters for transformation were optimized using the gene encoding β-glucuronidase (gusA) as the reporter gene. Statistical analysis revealed that helium pressure, target distance, and osmotic pretreatme ...
DOI:
10.1016/j.sajb.2019.07.037

2. Analysis of activity driven by upstream regulatory modules (URM) of tapetum specific genes TA29 and A9 at ectopic locations in tobacco transgenics

Author:
Preeti Apurve Sharma; Neetu Verma; Pradeep Kumar Burma
Source:
Journal of plant biochemistry and biotechnology 2018 v.27 no.4 pp. 443-452
ISSN:
0971-7811
Subject:
5' untranslated regions; Arabidopsis thaliana; Nicotiana tabacum; adverse effects; beta-glucuronidase; leaves; meristems; reporter genes; restorer lines; roots; tobacco; transgenic plants
Abstract:
... TA29 and A9 are genes from Nicotiana tabacum and Arabidopsis thaliana respectively, which express in a tapetum specific manner. The upstream regulatory modules (URMs; i.e. the promoter and the 5′UTR) of these genes have been used in development of male sterile and restorer lines expressing the barnase and barstar genes for hybrid seed production. While initial studies show that these URMs drive th ...
DOI:
10.1007/s13562-018-0453-y

3. Cloning and functional characterization of epidermis-specific promoter MtML1 from Medicago truncatula

Author:
Li Gao; Ye Tian; Meng-Ci Chen; Li Wei; Tian-Ge Gao; Hong-Ju Yin; Jin-Lin Zhang; Tanweer Kumar; Lin-Bo Liu; Suo-Min Wang
Source:
Journal of biotechnology 2019 v.300 pp. 32-39
ISSN:
0168-1656
Subject:
Arabidopsis thaliana; Medicago truncatula; Nicotiana tabacum; TATA box; apical meristems; beta-glucuronidase; fruits; gene expression; genes; genetic engineering; leaves; legumes; models; roots
Abstract:
... Epidermis-specific promoters are necessary for ectopic expression of specific functional genes such as the cuticle-related genes. Previous studies indicated that both ECERIFERUM 6 (AtCER6) and MERISTEM L1 LAYER (ATML1) promoters from Arabidopsis thaliana can drive gene expression specifically in the epidermis of shoot apical meristems (SAMs) and leaves. However, the epidermis-specific promoters fr ...
DOI:
10.1016/j.jbiotec.2019.05.003

4. Whitefly and aphid inducible promoters of Arabidopsis thaliana L.

Author:
Neeraj Kumar Dubey; Devesh Kumar Mishra; Asif Idris; Deepti Nigam; Pradhyumna Kumar Singh; Samir V. Sawant
Source:
Journal of genetics 2018 v.97 no.1 pp. 109-119
ISSN:
0022-1333
Subject:
1-aminocyclopropane-1-carboxylic acid; Aleyrodidae; Aphidoidea; Arabidopsis thaliana; Nicotiana tabacum; abscisic acid; beta-glucuronidase; databases; genetic vectors; information sources; jasmonic acid; microarray technology; promoter regions; proteins; quantitative polymerase chain reaction; reporter genes; reverse transcriptase polymerase chain reaction; salicylic acid; tobacco; transcription (genetics); transgenic plants
Abstract:
... Lack of regulated expression and tissue specificity are the major drawbacks of plant and virus-derived constitutive promoters. A precise tissue or site-specific expression, facilitate regulated expression of proteins at the targeted time and site. Publically available microarray data on whitefly and aphid infested Arabidopsis thaliana L. was used to identify whitefly and aphid-inducible genes. The ...
DOI:
10.1007/s12041-018-0887-y

5. Characterization and functional analysis of GhWRKY42, a group IId WRKY gene, in upland cotton (Gossypium hirsutum L.)

Author:
Lijiao Gu; Hengling Wei; Hantao Wang; Junji Su; Shuxun Yu
Source:
BMC genetics 2018 v.19 no.1 pp. 48
ISSN:
1471-2156
Subject:
Arabidopsis; Gossypium hirsutum; Nicotiana tabacum; abiotic stress; beta-glucuronidase; chlorophyll; cotton; gene expression; gene silencing; genes; leaves; models; phenotype; plant height; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; roots; stems; stress response; transactivators; transcriptional activation; transgenic plants; yeasts
Abstract:
... BACKGROUND: WRKY transcription factors (TFs) participate in various physiological processes of plants. Although WRKY genes have been well studied in model plants, knowledge of the functional roles of these genes is still extremely limited in cotton. RESULTS: In this study, a group IId WRKY gene from cotton, GhWRKY42, was isolated and characterized. Our data showed that GhWRKY42 localized to the nu ...
DOI:
10.1186/s12863-018-0653-4
PubMed:
30060731
PubMed Central:
PMC6065155

6. Identification and functional characterization of the MdHB-1 gene promoter sequence from Malus×domestica

Author:
Hao-jie WANG; Yong-hua JIANG; Ying-wei QI; Jie-yu DAI; Yan-li LIU; Xian-bo ZHU; Cui-hua LIU; Yan-rong LÜ; Xiao-lin REN
Source:
Journal of integrative agriculture 2017 v.16 no.8 pp. 1730-1741
ISSN:
2095-3119
Subject:
Malus domestica; Nicotiana tabacum; Pseudomonas syringae pv. tomato; abscisic acid; apples; beta-glucuronidase; bioactive properties; bioinformatics; ethephon; ethylene; genes; gibberellic acid; hormones; nucleotides; promoter regions; ripening; salicylic acid; sequence analysis; stress response; transcription (genetics); transcription factors
Abstract:
... Homeobox 1 in Malus×domestica (MdHB-1) is a transcription factor that belongs to homeodomain-leucine zipper I (HD-Zip I) protein subfamily. According to previous reports, MdHB-1 could regulate ethylene synthesis by binding with the MdACO1 promoter, but other functions of MdHB-1 are still unknown. To reveal more clues concerning the characters of the MdHB-1 gene promoter and the functions of MdHB-1 ...
DOI:
10.1016/S2095-3119(16)61548-4

7. TcPHYLL, a cacao phylloplanin expressed in young tissues and glandular trichomes

Author:
Lais Freire; Juliano Oliveira Santana; Aurizangela Oliveira de Sousa; Juliana Bispo dos Santos; Ivina Barbosa de Oliveira; Fátima Cerqueira Alvim; Karina Peres Gramacho; Marcio G.C. Costa; Carlos Priminho Pirovani
Source:
Physiological and molecular plant pathology 2017 v.100 pp. 126-135
ISSN:
0885-5765
Subject:
Moniliophthora perniciosa; Nicotiana tabacum; Peronospora tabacina; Theobroma cacao; allergenicity; amino acids; anti-infective agents; apical meristems; beta-glucuronidase; bioinformatics; databases; flowers; genome; glycosylation; leaf primordia; open reading frames; phosphorylation; phylloplane; pollen proteins; quantitative polymerase chain reaction; regulatory sequences; reverse transcriptase polymerase chain reaction; signal peptide; tobacco; transgenic plants; trees; trichomes
Abstract:
... Phylloplanins are potential antimicrobial compounds that can be found on the surface of some plants. These compounds were initially identified in Nicotiana tabacum as proteins secreted by short glandular trichomes (STG), which play an important role in controlling infection caused by Peronospora tabacina, especially by acting on the phylloplane, the first site of plant-pathogen interaction. A prot ...
DOI:
10.1016/j.pmpp.2017.06.002

8. Tissue-Preferential Activity and Induction of the Pepper Capsaicin Synthase PUN1 Promoter by Wounding, Heat and Metabolic Pathway Precursor in Tobacco and Tomato Plants

Author:
Justin Kirke; Noah Kaplan; Stephanie Velez; Xiao-Lu Jin; Paveena Vichyavichien; Xing-Hai Zhang
Source:
Molecular biotechnology 2018 v.60 no.3 pp. 194-202
ISSN:
1073-6085
Subject:
tomatoes; Arabidopsis; fruits; staining; transgenic plants; capsaicin; heat; flowers; biochemical pathways; gene expression; Nicotiana tabacum; genes; phenylalanine; tobacco; pollen; promoter regions; beta-glucuronidase; leaves; Solanum lycopersicum var. lycopersicum
Abstract:
... A promoter is an essential structural component of a gene that controls its transcription activity in different development stages and in response to various environmental stimuli. Knowledge of promoter functionality in heterologous systems is important in the study of gene regulation and biotechnological application. In order to explore the activity of the pepper capsaicin synthase gene (PUN1) pr ...
DOI:
10.1007/s12033-018-0060-0

9. Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis

Author:
Carolina S. Costa; Juliana P. Bravo; Cíntia L. Ribeiro; Adriana S. Soprano; Flávio T. Sassaki; Ivan G. Maia
Source:
Plant cell, tissue, and organ culture 2017 v.131 no.2 pp. 213-222
ISSN:
0167-6857
Subject:
Eucalyptus grandis; Nicotiana tabacum; beta-glucuronidase; gene expression; histology; hypocotyls; leaves; phloem; potassium; promoter regions; reporter genes; roots; staining; starvation; tobacco; transgenic plants; transporters
Abstract:
... The construction of expression cassettes harboring tissue-specific promoters is a viable alternative to limit transgene expression to specific organs and cell types. In this study, we have functionally characterized the promoter of a Eucalyptus grandis gene encoding a putative high-affinity HAK5-like potassium (K⁺) transporter (designated EgHAK5) showing root-specific expression. The ability of th ...
DOI:
10.1007/s11240-017-1276-6

10. The pathogen-inducible promoter of defense-related LsGRP1 gene from Lilium functioning in phylogenetically distinct species of plants

Author:
Chia-Hua Lin; Chao-Ying Chen
Source:
Plant science 2017 v.254 pp. 22-31
ISSN:
0168-9452
Subject:
regulatory sequences; biotic stress; plant hormones; Nicotiana benthamiana; cold stress; phylogeny; gene expression; Lilium; Nicotiana tabacum; ferns and fern allies; genes; Gymnospermae; beta-glucuronidase; pathogens
Abstract:
... A suitable promoter greatly enhances the efficiency of target gene expression of plant molecular breeding and farming; however, only very few promoters are available for economically important non-graminaceous ornamental monocots. In this study, an 868-bp upstream region of defense-related LsGRP1 of Lilium, named PLsGRP1, was cloned by genome walking and proven to exhibit promoter activity in Nico ...
DOI:
10.1016/j.plantsci.2016.10.006

11. An AGAMOUS intron‐driven cytotoxin leads to flowerless tobacco and produces no detrimental effects on vegetative growth of either tobacco or poplar

Author:
Wei Li; Wei Hu; Chu Fang; Longzheng Chen; Weibing Zhuang; Lorenzo Katin‐Grazzini; Richard J. McAvoy; Karl Guillard; Yi Li
Source:
Plant biotechnology journal 2016 v.14 no.12 pp. 2276-2287
ISSN:
1467-7644
Subject:
cytotoxins; buds; phenotype; roots; transgenic plants; castration; Populus trichocarpa; gene flow; growing season; Nicotiana tabacum; vegetative growth; field experimentation; biomass; introns; promoter regions; beta-glucuronidase; tobacco; shoots
Abstract:
... Flowerless trait is highly desirable for poplar because it can prevent pollen‐ and seed‐mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTAG2I (forward orientation of the PTAG2 intron sequence) and rPTAG2I (reverse orien ...
DOI:
10.1111/pbi.12581
PubMed:
27168170
PubMed Central:
PMC5103258

12. Deletion and hormone induction analyses of the 4-coumarate: CoA ligase gene promoter from Pennisetum purpureum in transgenic tobacco plants

Author:
Xiao-Qun Peng; Shan-Wen Ke; Jia-Qi Liu; Shu Chen; Tian-Xiu Zhong; Xin-Ming Xie
Source:
Plant cell, tissue, and organ culture 2016 v.126 no.3 pp. 439-448
ISSN:
0167-6857
Subject:
TATA box; quantitative analysis; staining; stems; transgenic plants; Agrobacterium radiobacter; bioinformatics; Nicotiana tabacum; Cenchrus purpureus; molecular cloning; reporter genes; abscisic acid; tobacco; xylem; gel electrophoresis; roots; beta-glucuronidase; leaves
Abstract:
... 4-coumarate: coenzyme A ligase (4CL) is a key enzyme in phenylpropanoid synthesis pathway. Bioinformatic analysis revealed that Pennisetum purpureum 4CL (Pp4CL) promoter contains TATA-box, CAAT-box, GC-box, TATC-box, ABRE, TGACG-motif and WBOXNTERF3. The Pp4CL promoter together with β-glucuronidase (GUS) reporter gene was cloned into pCAMBIA1305.1 vector, and transformed Nicotiana tabacum using Ag ...
DOI:
10.1007/s11240-016-1012-7

13. Molecular cloning and characterization of the trichome specific chrysanthemyl diphosphate/chrysanthemol synthase promoter from Tanacetum cinerariifolium

Author:
Shirin Sultana; Hao Hu; Liping Gao; Jing Mao; Jing Luo; Maarten A. Jongsma; Caiyun Wang
Source:
Scientia horticulturae 2015 v.185 pp. 193-199
ISSN:
0304-4238
Subject:
Chrysanthemum morifolium; DNA; Nicotiana tabacum; Tanacetum cinerariifolium; adverse effects; beta-glucuronidase; biosynthesis; flowers; fluorescence; fruits; green fluorescent protein; growth and development; hormones; metabolic engineering; molecular cloning; pesticides; plant growth; pyrethrins; reporter genes; research support; staining; tobacco; trichomes
Abstract:
... Natural pyrethrins accumulate to high concentrations in the flower achenes of pyrethrum (Tanacetum cinerariifolium). They are extracted from dried flowers and widely used as a natural pesticide. Chrysanthemol synthase (CHS) is the first key enzyme in the biosynthesis of pyrethrins. In this work, a 1128bp TcCHS promoter fragment was cloned from pyrethrum genomic DNA. The sequence contained cis-elem ...
DOI:
10.1016/j.scienta.2015.01.032

14. A Novel PhLRR Gene Promoter is Sufficient for Engineering Male Sterility in Petunia

Author:
Yuanzheng Yue; Di Yang; Jian Sun; Hao Peng; Chaoqun Yin; Rui Guo; Guogui Ning; Huirong Hu
Source:
Plant molecular biology reporter 2016 v.34 no.5 pp. 970-977
ISSN:
0735-9640
Subject:
engineering; ornamental plants; plant breeding; Petunia hybrida; staining; transgenic plants; developmental stages; early development; female fertility; Nicotiana tabacum; genes; male fertility; pollen; promoter regions; beta-glucuronidase; tobacco
Abstract:
... Tissue-specific promoters can drive genes specifically expressed in the target organs and have been widely used in plant molecular breeding. In this study, a 1.2-kb promoter region of an anther-specific gene PhLRR from Petunia hybrida “Fantasy” was isolated and fused to the β-glucuronidase (GUS) gene. The pPhLRR::GUS vector was heterogeneously transformed into tobacco in which the GUS staining was ...
DOI:
10.1007/s11105-016-0977-z

15. A Cassava vein mosaic virus promoter cassette induces high and stable gene expression in clonally propagated transgenic cassava (Manihot esculenta Crantz)

Author:
Olufemi O. Oyelakin; Jelili T. Opabode; Adebola A. Raji; Ivan L. Ingelbrecht
Source:
South African journal of botany 2015 v.97 pp. 184-190
ISSN:
0254-6299
Subject:
Cassava vein mosaic virus; Cauliflower mosaic virus; Manihot esculenta; Nicotiana tabacum; beta-glucuronidase; cassava; fibrous roots; gene expression; gene silencing; genetic markers; genetic transformation; greenhouse production; greenhouses; kanamycin; landraces; petioles; ratooning; reporter genes; roots; tissues; tobacco; transfer DNA; transgenes; transgenic plants; tubers; vegetative propagation
Abstract:
... The study described a T-DNA vector with a Cassava vein mosaic virus promoter cassette (pCsVMV) and a kanamycin selectable marker gene driven by the 35S Cauliflower mosaic virus promoter with a view to stably express transgenes over repeated cycles of clonal propagation. A β-glucuronidase reporter gene under control of pCsVMV (pCsVMV-GUS) was introduced into the cassava landrace ‘Tokunbo’ via Agrob ...
DOI:
10.1016/j.sajb.2014.11.011

16. Lipid transfer proteins in coffee: isolation of Coffea orthologs, Coffea arabica homeologs, expression during coffee fruit development and promoter analysis in transgenic tobacco plants

Author:
Michelle G. Cotta; Leila M. G. Barros; Juliana D. de Almeida; Fréderic de Lamotte; Eder A. Barbosa; Natalia G. Vieira; Gabriel S. C. Alves; Felipe Vinecky; Alan C. Andrade; Pierre Marraccini
Source:
Plant molecular biology 2014 v.85 no.1-2 pp. 11-31
ISSN:
0167-4412
Subject:
Coffea arabica; Coffea canephora; Nicotiana tabacum; Northern blotting; beta-glucuronidase; complementary DNA; early development; endosperm; fruiting; fruits; genomics; isoelectric point; molecular weight; promoter regions; proteins; reporter genes; single nucleotide polymorphism; tobacco
Abstract:
... The aim of the present study was to perform a genomic analysis of non-specific lipid-transfer proteins (nsLTPs) in coffee. Several nsLTPs-encoding cDNA and gene sequences were cloned from Coffea arabica and Coffea canephora species. In this work, their analyses revealed that coffee nsLTPs belong to Type II LTP characterized under their mature forms by a molecular weight of around 7.3 kDa, a basic ...
DOI:
10.1007/s11103-013-0166-5
PubMed:
24469961

17. Manipulation of monoubiquitin improves chilling tolerance in transgenic tobacco (Nicotiana tabacum)

Author:
Yanan Feng; Meng Zhang; Qifang Guo; Guokun Wang; Jiangfeng Gong; Ying Xu; Wei Wang
Source:
Plant physiology and biochemistry 2014 v.75 pp. 138-144
ISSN:
0981-9428
Subject:
electrolytes; reactive oxygen species; wheat; genes; sugars; malondialdehyde; proline; vector control; solutes; Triticum aestivum; Nicotiana tabacum; leaves; gene overexpression; ubiquitin; enzyme activity; tobacco; proteasome endopeptidase complex; wilting; transgenic plants; cold stress; photosystem II; cold tolerance; beta-glucuronidase
Abstract:
... Ubiquitin (Ub) is a multifunctional protein that mainly functions to tag proteins for selective degradation by the 26S proteasome. We cloned an Ub gene TaUb2 from wheat (Triticum aestivum L.) previously. To study the function of TaUB2 in chilling stress, sense and antisense Ub transgenic tobacco plants (Nicotiana tabacum L.), as well as wild type (WT) and vector control β-glucuronidase (T-GUS) pla ...
DOI:
10.1016/j.plaphy.2013.11.003

18. Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum

Author:
Xuelian Zheng; Qian Li; Dongqing Liu; Lili Zang; Kaiyue Zhang; Kejun Deng; Shixin Yang; Zhengyang Xie; Xu Tang; Yiping Qi; Yong Zhang
Source:
Plant cell reports 2015 v.34 no.11 pp. 1873-1884
ISSN:
0721-7714
Subject:
fluorometry; chemical elements; stems; sweet potatoes; assays; roots; sucrose; transgenic plants; tandem repeat sequences; starch; Ipomoea batatas; gene expression; glucose-1-phosphate adenylyltransferase; Nicotiana tabacum; tissues; reporter genes; tobacco; promoter regions; beta-glucuronidase; leaves
Abstract:
... KEY MESSAGE : The IbAGP1 gene of sweet potato (Ipomoea batatas) encodes the sucrose-inducible small subunit of ADP-glucose pyrophosphorylase. Through expression analysis of 5′-truncations and synthetic forms of the IbAGP1 promoter in transgenic tobacco, we show that SURE-Like elements and W-box elements of the promoter contribute to the sucrose inducibility of this gene. Sweet potato (Ipomoea bata ...
DOI:
10.1007/s00299-015-1834-5

19. Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding

Author:
Xiuping Zou; Erling Song; Aihong Peng; Yongrui He; Lanzhen Xu; Tiangang Lei; Lixiao Yao; Shanchun Chen
Source:
Plant cell, tissue, and organ culture 2014 v.117 no.1 pp. 85-98
ISSN:
0167-6857
Subject:
Cauliflower mosaic virus; Citrus sinensis; Nicotiana tabacum; Xanthomonas axonopodis pv. citri; beta-glucuronidase; disease resistance; fluorometry; pathogens; potatoes; reporter genes; tobacco; transgenes
Abstract:
... To improve the resistance of citrus to canker disease caused by Xanthomonas axonopodis pv. citri (Xac), it is important to identify gene promoters that are specifically induced by pathogen infection. Here, we evaluated the functionality of PPP1 and hsr203J (Nicotiana tabacum L.) and gst1 (potato) pathogen-inducible promoters to drive expression of the β-glucuronidase (GUS) reporter gene in transge ...
DOI:
10.1007/s11240-013-0423-y

20. Characterization of the GLP13 gene promoter in Arabidopsis thaliana

Author:
L. Yang; T. Li; S. C. Zhang; G. L. Gao; C. W. Yang
Source:
Biologia plantarum 2013 v.57 no.2 pp. 231-237
ISSN:
0006-3134
Subject:
Arabidopsis thaliana; Nicotiana tabacum; beta-glucuronidase; phloem; plant development; proteins; reporter genes; tobacco; transgenic plants; vascular bundles
Abstract:
... In transgenic plants, for many applications it is important that the inserted genes are expressed in a tissue-specific manner. This in turn could help better understanding their roles in plant development. Germin-like proteins (GLPs) play diverse roles in plant development and defense responses. In order to understand the functions and regulation of the GLP13 gene, its promoter (762 bp) was cloned ...
DOI:
10.1007/s10535-012-0273-1

21. Functional Analysis of the Promoter of a Glycosyl Hydrolase Gene Induced in Resistant Sinapis alba by Alternaria brassicicola

Author:
Chatterjee Madhuvanti; Mazumder Mrinmoy; Basu Debabrata
Source:
Phytopathology 2013 v.103 no.8 pp. 841-850
ISSN:
0031-949X
Subject:
Alternaria brassicicola; Brassica juncea; Nicotiana tabacum; Sinapis alba; abscisic acid; adverse effects; beta-glucuronidase; gene expression; gene expression regulation; genes; genetically modified organisms; jasmonic acid; salicylic acid
Abstract:
... A putative family 3 glycosyl hydrolase (GH) gene showed significant differential expression in resistant Sinapis alba, compared with the susceptible Brassica juncea, as part of the initial responses during interaction with the necrotroph Alternaria brassicicola. To understand the mechanism of induction, the promoter was isolated and deletion analysis carried out. All the promoter fragments were fu ...
DOI:
10.1094/PHYTO-11-12-0303-R

22. Isolation and molecular characterization of GmERF7, a soybean ethylene-response factor that increases salt stress tolerance in tobacco

Author:
Ying Zhai; Ying Wang; Yanjie Li; Tingting Lei; Fan Yan; Liantai Su; Xiaowei Li; Yan Zhao; Xin Sun; Jingwen Li; Qingyu Wang
Source:
Gene 2013 v.513 pp. 174-183
ISSN:
0378-1119
Subject:
Glycine max; Nicotiana tabacum; abscisic acid; amino acids; beta-glucuronidase; carbohydrates; chlorophyll; cold; cold treatment; drought; ethylene; gene expression; leaves; malondialdehyde; methyl jasmonate; nuclear localization signals; onions; plant response; reporter genes; salt stress; salt tolerance; sequence analysis; soybeans; stress tolerance; tobacco; transcription factors; transcriptional activation; transgenic plants
Abstract:
... Ethylene-response factors (ERFs) play an important role in regulating gene expression in plant responses to biotic and abiotic stresses. In this study, a new ERF transcription factor, GmERF7, was isolated from soybean. Sequence analysis showed that GmERF7 contained an AP2/ERF domain with 58 amino acids, two putative nuclear localization signal (NLS) domains, an acidic amino acid-rich transcription ...
DOI:
10.1016/j.gene.2012.10.018
PubMed:
23111158

23. NtPDR1, a plasma membrane ABC transporter from Nicotiana tabacum, is involved in diterpene transport

Author:
Jérôme Crouzet; Julien Roland; Emmanuel Peeters; Tomasz Trombik; Eric Ducos; Joseph Nader; Marc Boutry
Source:
Plant molecular biology 2013 v.82 no.1-2 pp. 181-192
ISSN:
0167-4412
Subject:
ABC transporters; Nicotiana tabacum; Western blotting; active transport; antibodies; beta-glucuronidase; cell suspension culture; diterpenoids; flowers; gene expression; hormones; immunocytochemistry; immunohistochemistry; leaves; metabolites; methyl jasmonate; naphthaleneacetic acid; plasma membrane; promoter regions; reporter genes; roots; stems; tobacco; transgenic plants; trichomes
Abstract:
... ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tabacum suspension cells (BY2), in which its expression was induced by microbial eli ...
DOI:
10.1007/s11103-013-0053-0
PubMed:
23564360

24. Promoters of HcTPS1 and HcTPS2 Genes from Hedychium coronarium Direct Floral-Specific, Developmental-Regulated and Stress-Inducible Gene Expression in Transgenic Tobacco

Author:
Xin-Yue Li; Shao-Yuan Zheng; Rang-Cai Yu; Yan-Ping Fan
Source:
Plant molecular biology reporter 2014 v.32 no.4 pp. 864-880
ISSN:
0735-9640
Subject:
Hedychium coronarium; Nicotiana tabacum; abiotic stress; beta-glucuronidase; breeding; flowering; fluorescence; gene expression; horticultural crops; insects; jasmonic acid; methyl jasmonate; odors; ornamental horticulture; plant hormones; plant stress; promoter regions; reporter genes; stress tolerance; transgenic plants
Abstract:
... HcTPS1 and HcTPS2 are floral-scent genes that mediate terpene formation in Hedychium coronarium. Transcription of these genes is induced by various abiotic stresses and hormonal stimuli. In this study, the regulatory mechanisms responsible for HcTPS1 and HcTPS2 expression were investigated by analyzing their promoters. Two genomic fragments, 1,298-bp and 1,928-bp in length, upstream of HcTPS1 and ...
DOI:
10.1007/s11105-013-0697-6

25. HopX1 in Erwinia amylovora Functions as an Avirulence Protein in Apple and Is Regulated by HrpL

Author:
A. M. Bocsanczy; D. J. Schneider; G. A. DeClerck; S. Cartinhour; S. V. Beer
Source:
Journal of bacteriology 2012 v.194 no.3 pp. 553-560
ISSN:
0021-9193
Subject:
Erwinia amylovora; apples; Nicotiana benthamiana; plasmids; genes; sigma factors; shoots; pears; Nicotiana tabacum; type III secretion system; virulence; pathogens; Gram-negative bacteria; gene overexpression; Markov chain; virulent strains; hypersensitivity; bacteriology; mutants; nucleotide sequences; beta-glucuronidase
Abstract:
... Fire blight is a devastating disease of rosaceous plants caused by the Gram-negative bacterium Erwinia amylovora. This pathogen delivers virulence proteins into host cells utilizing the type III secretion system (T3SS). Expression of the T3SS and of translocated and secreted substrates is activated by the alternative sigma factor HrpL, which recognizes hrp box promoters upstream of regulated genes ...
DOI:
10.1128/JB.05065-11
PubMed:
22123252
PubMed Central:
PMC3264070

26. Isolation and identification of promoter elements of Cotton leaf curl Multan betasatellite associated with Tomato leaf curl viruses

Author:
Richa Shukla; Sunita Dalal; V.G. Malathi
Source:
Archives of phytopathology and plant protection 2013 v.46 no.9 pp. 1015-1029
ISSN:
1477-2906
Subject:
Nicotiana benthamiana; Nicotiana tabacum; Tomato leaf curl New Delhi virus; Tomato leaf curl virus; beta-glucuronidase; cotton; hosts; leaf curling; leaves; pathogenicity; polymerase chain reaction; promoter regions; reporter genes; stop codon; tomatoes; viruses
Abstract:
... Betasatellite DNAs associated with Old World monopartite begomoviruses encode a single gene (βC1) which mediate the vital functions required for viral pathogenicity. A region of upstream translation start site of βC1 of Cotton leaf curl Multan betasatellite (CLCuMB) associated with Tomato leaf curl New Delhi virus was tested for its promoter activity. Transcript mapping studies revealed that the t ...
DOI:
10.1080/03235408.2012.756175

27. Qualitative and quantitative analysis of the Nictaba promoter activity during development in Nicotiana tabacum

Author:
Annelies Delporte; Sofie Van Holle; Els J.M. Van Damme
Source:
Plant physiology and biochemistry 2013 v.67 pp. 162-168
ISSN:
0981-9428
Subject:
Arabidopsis thaliana; Nicotiana tabacum; beta-glucuronidase; cotyledons; fluorometry; jasmonic acid; leaves; lectins; quantitative analysis; reporter genes; roots; staining; tobacco; transgenic plants
Abstract:
... Nictaba is a genuine jasmonate inducible lectin expressed in the leaves and roots of Nicotiana tabacum cv. Samsun NN. Although the jasmonate pathway is generally highly conserved among plant species, recent research showed that it is followed by plant specific downstream processes. Previously the Nictaba promoter activity was studied in the model plant Arabidopsis thaliana, using the β-glucuronida ...
DOI:
10.1016/j.plaphy.2013.03.005

28. A cotton group C MAP kinase gene, GhMPK2, positively regulates salt and drought tolerance in tobacco

Author:
Liang Zhang; Dongmei Xi; Shanwei Li; Zheng Gao; Shuoli Zhao; Jing Shi; Changai Wu; Xingqi Guo
Source:
Plant molecular biology 2011 v.77 no.1-2 pp. 17-31
ISSN:
0167-4412
Subject:
Gossypium hirsutum; Nicotiana tabacum; Northern blotting; abscisic acid; amino acid metabolism; beta-glucuronidase; conserved sequences; cotton; drought tolerance; gene expression regulation; gene overexpression; genes; mitogen-activated protein kinase; polyethylene glycol; proline; promoter regions; salt tolerance; seed germination; sodium chloride; stress response; tobacco; transgenic plants; vegetative growth; water stress
Abstract:
... Mitogen-activated protein kinase (MAPK) cascades play important roles in mediating biotic and abiotic stress responses. In plants, MAPKs are classified into four major groups (A–D) according to their sequence homology and conserved phosphorylation motifs. Compared with well-studied MAPKs in groups A and B, little is known about group C. In this study, we functionally characterised a stress-respons ...
DOI:
10.1007/s11103-011-9788-7
PubMed:
21590508

29. Cellular and subcellular localization of flavin-monooxygenases involved in glucosinolate biosynthesis

Author:
Li, Jing; Kristiansen, Kim A.; Hansen, Bjarne G.; Halkier, Barbara A.
Source:
Journal of experimental botany 2011 v.62 no.3 pp. 1337-1346
ISSN:
0022-0957
Subject:
genes; promoter regions; stems; gene expression; secondary metabolites; Nicotiana tabacum; enzyme substrates; oxygenases; leaves; glucosinolates; Arabidopsis thaliana; phloem; epidermis (plant); enzyme activity; tobacco; green fluorescent protein; biosynthesis; bioactive properties; transgenic plants; biochemical pathways; gene fusion; beta-glucuronidase
Abstract:
... Glucosinolates are amino acid-derived secondary metabolites with diverse biological activities dependent on chemical modifications of the side chain. Five flavin-monooxygenases FMOGS₋OX₁₋₅ have recently been identified as aliphatic glucosinolate side chain modification enzymes in Arabidopsis thaliana that catalyse the generation of methylsulphinylalkyl glucosinolates, which can be hydrolysed to pr ...
DOI:
10.1093/jxb/erq369
PubMed:
21078824

30. Structural and functional characterization of a pollen-specific promoter NTPp13 in tobacco

Author:
Yang, A.; Zhang, S.; Liu, Sh.; Zhao, Q.; Pan, G.
Source:
Russian journal of genetics 2010 v.46 no.4 pp. 404-408
ISSN:
1022-7954
Subject:
Nicotiana tabacum; Zea mays; beta-glucuronidase; corn; pollen; pollen tubes; polymerase chain reaction; promoter regions; reporter genes; temperature; tobacco; transgenic plants
Abstract:
... A novel 407 bp nucleotide sequence NTPp13 was isolated from tobacco (Nicotiana tabacum L.) by PCR, its structure and function were characterized. The NTPp13 sequence was highly homologous with the pollen-specific expression promoter Zm13 from maize (Zea mays L.) and contained some key motifs which controlled pollen-specific expression. The NTPp13 was fused to the β-glucuronidase (GUS) reporter gen ...
DOI:
10.1134/S1022795410040034

31. Construction vascular-specific expression bi-directional promoters in plants

Author:
Lv, Xiaomeng; Song, Xiaodan; Rao, Guodong; Pan, Xiang; Guan, Liping; Jiang, Xiangning; Lu, Hai
Source:
Journal of biotechnology 2009 v.141 no.3-4 pp. 104-108
ISSN:
0168-1656
Subject:
Nicotiana tabacum; tobacco; genetic transformation; plasmid vectors; promoter regions; Phaseolus vulgaris; beans; plant proteins; cell wall components; ligases; gene expression; reporter genes; green fluorescent protein; beta-glucuronidase; plant vascular system
Abstract:
... Promoters that have been widely used for both basic research and biotechnological application in plants are generally unidirectional. Here we describe a strategy to bi-directionalize the vascular-specific expression grp1.8 promoter (here named GRPp) and 4CL1 promoter (here named 4CL1p) so that one promoter can direct the vascular-specific expression of two genes, one on each end of the promoter. T ...
DOI:
10.1016/j.jbiotec.2009.03.009
PubMed:
19433212

32. Dicistronic binary vector system—A versatile tool for gene expression studies in cell cultures and plants

Author:
Ali, Zahid; Schumacher, Heinz Martin; Heine-Dobbernack, Elke; El-Banna, Antar; Hafeez, Fauzia Yusuf; Jacobsen, Hans-Jörg; Kiesecker, Heiko
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 9-16
ISSN:
0168-1656
Subject:
genetic transformation; salt tolerance; recombinant DNA; peas; transgenic plants; Agrobacterium radiobacter; luciferase; plasmid vectors; gene expression; antiporters; Nicotiana tabacum; sodium; reporter genes; Pisum sativum; promoter regions; beta-glucuronidase; tobacco
Abstract:
... Dicistronic binary vector constructs based on pGreenII vectors for Agrobacterium mediated gene transfer alleviate the translational expression monitoring of a target gene in plants. The functionality of the transformation vectors was proven by marker gene constructs containing a mannopine synthase promoter (p-MAS) fused to a beta-glucuronidase (gus) gene followed by an internal ribosome entry site ...
DOI:
10.1016/j.jbiotec.2009.10.002
PubMed:
19835918

33. Isolation and Characterization of a Curcin Promoter from Jatropha curcas L. and Its Regulation of Gene Expression in Transgenic Tobacco Plants

Author:
Qin, Xiaobo; Zhang, Jinping; Shao, Caixia; Lin, Sha; Jiang, Luding; Zhang, Shuwen; Xu, Ying; Chen, Fang
Source:
Plant molecular biology reporter 2009 v.27 no.3 pp. 275-281
ISSN:
0735-9640
Subject:
Jatropha curcas; transgenic plants; gene expression; endosperm; Nicotiana tabacum; molecular cloning; promoter regions; beta-glucuronidase; tobacco; seed development
Abstract:
... Ribosome-inactivating proteins (RIPs) represent those proteins that universally depurinate conserved α-sarcin loops of large rRNAs. In this study, a 0.6-kb fragment of a 5' flanking region preceding a curcin gene, encoding a type I RIP curcin, of Jatropha curcas L. endosperm was cloned, and its regulation of expression of the β-glucuronidase (GUS) reporter gene was investigated in transgenic tobac ...
DOI:
10.1007/s11105-008-0078-8

34. Salicylic acid and methyl jasmonate but not Rose Bengal enhance artemisinin production through invoking burst of endogenous singlet oxygen

Author:
Guo, Xiao-Xia; Yang, Xue-Qin; Yang, Rui-Yi; Zeng, Qing-Ping
Source:
Plant science 2010 v.178 no.4 pp. 390-397
ISSN:
0168-9452
Subject:
Nicotiana tabacum; tobacco; artemisinin; transgenic plants; salicylic acid; methyl jasmonate; free radicals; endogenous sources; oxidative stress; promoter regions; carbon-oxygen lyases; hydrogen peroxide; beta-glucuronidase; reporter genes; phenotype; Artemisia annua; broadleaf weeds; exogenous sources
Abstract:
... As a cis element responsive to oxidative stresses, the promoter of amorphadiene synthase gene (pADS) from Artemisia annua was also found to be inducible by salicylic acid (SA) and methyl jasmonate (MJ), which had been evident by phenotyping pADS-driven β-glucuronidase reporter gene (GUS) in transgenic Nicotiana tabacum incubated with SA/MJ. Furthermore, SA/MJ-treated A. annua exhibited a correlati ...
DOI:
10.1016/j.plantsci.2010.01.014

35. Tomato mitogen activated protein kinases regulate the expression of extracellular invertase Lin6 in response to stress related stimuli

Author:
Hyun, Tae Kyung; Hoffmann, Anja; Sinha, Alok K.; Roitsch, Thomas
Source:
Functional plant biology 2009 v.36 no.12 pp. 1088-1097
ISSN:
1445-4408
Subject:
signal transduction; Solanum peruvianum; plant pathogenic fungi; mitogen-activated protein kinase; Fusarium oxysporum f. sp. lycopersici; plant proteins; tomatoes; carbohydrate metabolism; fungal diseases of plants; vegetable crops; gene expression; Nicotiana tabacum; gene expression regulation; messenger RNA; phloem; resistance mechanisms; beta-fructofuranosidase; salt stress; tobacco; transgenic plants; biochemical pathways; beta-glucuronidase
Abstract:
... Activation of mitogen-activated protein kinases (MAPKs) is a common reaction of plant cells in defence-related signal transduction pathways. Since the downstream events after the activation of MAPKs are largely unknown in plants, the role of MAPKs in the coordinate regulation of defence reactions and primary carbon metabolism by stress related stimuli has been analysed in tomato (Lycopersicon peru ...
DOI:
10.1071/FP09136

36. Activity of the AtMRP3 promoter in transgenic Arabidopsis thaliana and Nicotiana tabacum plants is increased by cadmium, nickel, arsenic, cobalt and lead but not by zinc and iron

Author:
Zientara, Katarzyna; Wawrzyńska, Anna; Łukomska, Jolanta; López-Moya, José Rafael; Liszewska, Frantz; Assunção, Ana G.L.; Aarts, Mark G.M.; Sirko, Agnieszka
Source:
Journal of biotechnology 2009 v.139 no.3 pp. 258-263
ISSN:
0168-1656
Subject:
Arabidopsis thaliana; Nicotiana tabacum; tobacco; transgenic plants; gene expression; promoter regions; ABC transporters; reporter genes; beta-glucuronidase; cadmium; nickel; cobalt; lead; zinc; iron; roots; shoots; phytoremediation
Abstract:
... Characterization of the function, regulation and metal-specificity of metal transporters is one of the basic steps needed for the understanding of transport and accumulation of toxic metals and metalloids by plants. In this work GUS was used as a reporter for monitoring the activity of the promoter of the AtMRP3 gene from Arabidopsis thaliana, a gene encoding an ABC-transporter, expression of whic ...
DOI:
10.1016/j.jbiotec.2008.12.001

37. CYP82E4-mediated nicotine to nornicotine conversion in tobacco is regulated by a senescence-specific signaling pathway

Author:
Chakrabarti, Manohar; Bowen, Steven W.; Coleman, Nicholas P.; Meekins, Karen M.; Dewey, Ralph E.; Siminszky, Balazs
Source:
Plant molecular biology 2008 v.66 no.4 pp. 415-427
ISSN:
0167-4412
Subject:
ethylene; yeast extract; drought; genes; salicylic acid; promoter regions; plant damage; roots; Tobacco mosaic virus; stems; nicotine; jasmonic acid; gene expression; mature plants; Nicotiana tabacum; gene expression regulation; leaves; fluorometry; nornicotine; transcription (genetics); cytochrome P-450; enzyme activity; tobacco; loci; flowers; abscisic acid; beta-glucuronidase
Abstract:
... Nicotine to nornicotine conversion in tobacco (Nicotiana tabacum L.) is regulated by an unstable converter locus which in its activated state gives rise to a high nornicotine, low nicotine phenotype in the senescing leaves. In plants that carry the high nornicotine trait, nicotine conversion is primarily catalyzed by a cytochrome P450 protein, designated CYP82E4 whose transcription is strongly upr ...
DOI:
10.1007/s11103-007-9280-6
PubMed:
18196465

38. Effects of copy number of an octopine synthase enhancer element and its distance from the TATA box on heterologous expression in transgenic tobacco

Author:
Liu, Shijuan; Bao, Ying
Source:
Acta physiologiae plantarum 2009 v.31 no.4 pp. 705-710
ISSN:
0137-5881
Subject:
Nicotiana tabacum; TATA box; beta-glucuronidase; enhancer elements; gene dosage; gene expression; octopine; reporter genes; tobacco; transgenic plants
Abstract:
... The relationship among motif copy number, its distance from the TATA box and expression level was analyzed in transformed tobacco plants. Single or multiple copies of octopine synthase (ocs) enhancer elements from the ocs promoter were linked to the minimal ocs promoter and a β-glucuronidase (GUS) reporter gene, then transformed stably into tobacco. Reporter gene assays revealed that mere repetiti ...
DOI:
10.1007/s11738-009-0282-7

39. Functional characterization of a cotton late embryogenesis-abundant D113 gene promoter in transgenic tobacco

Author:
Luo, Keming; Zhang, Guofang; Deng, Wei; Luo, Fengtao; Qiu, Kun; Pei, Yan
Source:
Plant cell reports 2008 v.27 no.4 pp. 707-717
ISSN:
0721-7714
Subject:
Gossypium hirsutum; Nicotiana tabacum; abscisic acid; beta-glucuronidase; cold; cold treatment; cotton; gene expression; genes; messenger RNA; promoter regions; salinity; seedlings; seeds; tobacco; transgenic plants
Abstract:
... Previous studies have shown that mRNA and protein encoded by late embryogenesis-abundant (LEA) gene D113 from Gossypium hirsutum L. accumulate at high levels in mature seeds and also in response to abscisic acid (ABA) in young embryo. In this study, we studied the expression of four promoter 5' deletion constructs (-1383, -974, -578 and -158) of the LEA D113 gene fused to beta-glucuronidase (GUS). ...
DOI:
10.1007/s00299-007-0482-9
PubMed:
18066554

40. Identification of cis-regulatory sequences that activate transcription in the suspensor of plant embryos

Author:
Kawashima, Tomokazu; Wang, Xingjun; Henry, Kelli F.; Bi, Yuping; Weterings, Koen; Goldberg, Robert B.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.9 pp. 3627-3632
ISSN:
0027-8424
Subject:
Arabidopsis thaliana; Phaseolus coccineus; transgenic plants; embryo (plant); transcription (genetics); gene expression; Nicotiana tabacum; reporter genes; embryogenesis; promoter regions; beta-glucuronidase
Abstract:
... Little is known about the molecular mechanisms by which the embryo proper and suspensor of plant embryos activate specific gene sets shortly after fertilization. We analyzed the upstream region of the scarlet runner bean (Phaseolus coccineus) G564 gene to understand how genes are activated specifically within the suspensor during early embryo development. Previously, we showed that the G564 upstre ...
DOI:
10.1073/pnas.0813276106
PubMed:
19208800
PubMed Central:
PMC2638740

41. Local and systemic gene expression of sesquiterpene phytoalexin biosynthetic enzymes in plant leaves

Author:
Maldonado-Bonilla, Luis David; Betancourt-Jiménez, Martha; Lozoya-Gloria, Edmundo
Source:
European journal of plant pathology 2008 v.121 no.4 pp. 439-449
ISSN:
0929-1873
Subject:
Capsicum annuum; Nicotiana tabacum; beta-glucuronidase; biosynthesis; capsidiol; gene expression; hot peppers; hybrids; leaves; messenger RNA; metabolites; pathogens; phytoalexins; reactive oxygen species; reporter genes; start codon; tobacco; transcriptional activation; transgenic plants
Abstract:
... Production of antimicrobial metabolites known as phytoalexins is considered as one of the initial and main barriers to inhibit pathogen development in local infected aerial tissues. Capsidiol is the main bicyclic sesquiterpene phytoalexin in tobacco (Nicotiana tabacum) and chili pepper (Capsicum annuum). Production of 5-epi-aristolochene by the corresponding sesquiterpene cyclase enzymes is consid ...
DOI:
10.1007/s10658-007-9262-1

42. Rice DREB1B promoter shows distinct stress-specific responses, and the overexpression of cDNA in tobacco confers improved abiotic and biotic stress tolerance

Author:
Gutha, Linga Reddy; Reddy, Arjula R.
Source:
Plant molecular biology 2008 v.68 no.6 pp. 533-555
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; Nicotiana tabacum; Oryza sativa; Tobacco streak virus; abscisic acid; beta-glucuronidase; cold; cold stress; complementary DNA; free radical scavengers; gene expression; gene expression regulation; oxidative stress; plant response; promoter regions; reporter genes; rice; root growth; salicylic acid; seed germination; sodium chloride; stress response; stress tolerance; tobacco; transcription (genetics); transcription factors; transgenic plants; water stress
Abstract:
... CBF/DREB (C-repeat binding factor/dehydration responsive element binding factor) family of transcription factors in plants is reported to be associated with regulation of gene expression under stress conditions. Here, we report the functional characterization of a DREB transcription factor, DREB1B gene from rice (Oryza sativa ssp. indica). The OsDREB1B gene was differentially regulated at the tran ...
DOI:
10.1007/s11103-008-9391-8
PubMed:
18754079

43. Transfer of Plastid DNA to the Nucleus Is Elevated during Male Gametogenesis in Tobacco

Author:
Sheppard, Anna E.; Ayliffe, Michael A.; Blatch, Laura; Day, Anil; Delaney, Sven K.; Khairul-Fahmy, Norfarhana; Li, Yuan; Madesis, Panagiotis; Pryor, Anthony J.; Timmis, Jeremy N.
Source:
Plant physiology 2008 v.148 no.1 pp. 328-336
ISSN:
0032-0889
Subject:
Nicotiana tabacum; ancestry; beta-glucuronidase; chloroplast DNA; evolution; gametogenesis; germ cells; nuclear genome; organelles; ovules; parents; plant tissues; progeny; reciprocal crosses; reporter genes; screening; seedlings; somatic cells; tobacco; transposition (genetics)
Abstract:
... In eukaryotes, many genes were transferred to the nucleus from prokaryotic ancestors of the cytoplasmic organelles during endosymbiotic evolution. In plants, the transfer of genetic material from the plastid (chloroplast) and mitochondrion to the nucleus is a continuing process. The cellular location of a kanamycin resistance gene tailored for nuclear expression (35SneoSTLS2) was monitored in the ...
DOI:
10.1104/pp.108.119107
PubMed:
18660434
PubMed Central:
PMC2528088

44. Zn stress-induced inhibition of bean PvSR2-GUS fusion gene splicing is gene-specific in transgenic tobacco

Author:
Qi, Xiaoting; Cui, Qiuhong; Luo, Ying; Guo, Chunfen; Chai, Tuanyao
Source:
Journal of plant physiology 2009 v.166 no.11 pp. 1223-1227
ISSN:
0176-1617
Subject:
Phaseolus vulgaris; beans; seedlings; beta-glucuronidase; transgenes; introns; alternative splicing; zinc; cadmium; heavy metals; tobacco; Nicotiana tabacum; transgenic plants
Abstract:
... The stress-related gene no. 2 of Phaseolus vulgaris (PvSR2) is metal inducible and contains a single intron. Here, we report that Zn stress inhibited the splicing of the PvSR2-β-glucuronidase (GUS) fusion gene in a concentration- and time-dependent manner in tobacco seedlings. The inhibition appears to be specific for the PvSR2-GUS transgene: splicing of four endogenous tobacco genes was unaffecte ...
DOI:
10.1016/j.jplph.2009.01.010
PubMed:
19304342

45. The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 mediates environmental stress responses in plants

Author:
Hong, Jeum Kyu; Hwang, Byung Kook
Source:
Planta 2009 v.229 no.2 pp. 249-259
ISSN:
0032-0935
Subject:
ethylene; sweet peppers; seedlings; genes; mannitol; salicylic acid; promoter regions; Capsicum annuum; Agrobacterium radiobacter; gene activation; membrane proteins; Nicotiana tabacum; sodium; leaves; Arabidopsis thaliana; gene overexpression; methyl jasmonate; sodium chloride; hypersensitive response; tobacco; stress tolerance; genetic transformation; seed germination; plant stress; nitric oxide; transgenic plants; gene fusion; abscisic acid; beta-glucuronidase; Pseudomonas syringae pv. tabaci
Abstract:
... The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 was analyzed by an Agrobacterium-mediated transient expression assay in tobacco leaves. Several stress-related cis-acting elements (GT-1, W-box and ABRE) are located within the CaPIMP1 promoter. In tobacco leaf tissues transiently transformed with a CaPIMP1 promoter-β-glucuronidase (GUS) gene fusion, serially 5'-deleted CaPI ...
DOI:
10.1007/s00425-008-0824-z
PubMed:
18936963

46. Direct creation of marker-free tobacco plants from agroinfiltrated leaf discs

Author:
Jia, Hongge; Liao, Mingjun; Verbelen, Jean-Pierre; Vissenberg, Kris
Source:
Plant cell reports 2007 v.26 no.11 pp. 1961-1965
ISSN:
0721-7714
Subject:
Agrobacterium; Nicotiana tabacum; agroinfiltration; beta-glucuronidase; callus; explants; leaves; polymerase chain reaction; tissue culture; tissue repair; tobacco; transgenic plants
Abstract:
... Agroinfiltration is employed as a fast way to directly create marker-free transgenic tobacco plants. As an example for the efficiency of the method, Agrobacterium cells harboring a marker-free vector coding for β-glucuronidase (GUS) were infiltrated into the leaf discs of Nicotiana tabacum, which were then used as explants for marker-free plant regeneration by tissue culture. Through GUS staining, ...
DOI:
10.1007/s00299-007-0403-y
PubMed:
17637995

47. Isolation and characterization of Brittle2 promoter from Zea Mays and its comparison with Ze19 promoter in transgenic tobacco plants

Author:
Chen, Xiaoping; Wang, Zhangying; Wang, Jianhua; Wang, Maoyan; Zhao, Li; Wang, Guoying
Source:
Plant cell, tissue, and organ culture 2007 v.88 no.1 pp. 11-20
ISSN:
0167-6857
Subject:
carbohydrate metabolism; Zea mays; transgenic plants; embryo (plant); corn; starch; biochemical pathways; gene expression; endosperm; Nicotiana tabacum; nucleotidyltransferases; histochemistry; zein; gene expression regulation; promoter regions; beta-glucuronidase; tobacco
Abstract:
... ADP-glucose pyrophosphorylase (AGPase) represents a key regulatory step in starch synthesis. A 0.9 kb of 5' flanking region preceding Brittle2 gene, encoding the small subunit of maize endosperm AGPase, was cloned from maize genome and its expression pattern was studied via the expression of β-glucuronidase (GUS) gene in transgenic tobacco. Analysis of GUS activities showed that the 0.9 kb fragmen ...
DOI:
10.1007/s11240-006-9165-4

48. Polyethyleneimine precipitation versus anion exchange chromatography in fractionating recombinant β-glucuronidase from transgenic tobacco extract

Author:
Holler, C.; Vaughan, D.; Zhang, C.
Source:
Journal of chromatography 2007 v.1142 no.1 pp. 98-105
ISSN:
0021-9673
Subject:
Nicotiana tabacum; tobacco; transgenic plants; recombinant proteins; beta-glucuronidase; plant extracts; fractionation; chemical precipitation; ion exchange chromatography; biopharmaceuticals
DOI:
10.1016/j.chroma.2006.08.044

49. Secretion marker proteins and cell-wall polysaccharides move through different secretory pathways

Author:
Leucci, Maria Rosaria; Di Sansebastiano, Gian-Pietro; Gigante, Massimiliano; Dalessandro, Giuseppe; Piro, Gabriella
Source:
Planta 2007 v.225 no.4 pp. 1001-1017
ISSN:
0032-0935
Subject:
Nicotiana tabacum; beta-glucuronidase; cell walls; cellulose synthase; cytochalasin D; fluorescence; gene overexpression; green fluorescent protein; leaves; polysaccharides; protein secretion; protoplasts; rats; roots; seedlings; tobacco; transgenic plants
Abstract:
... The building up of the cell wall is tightly dependent on the functionality of the secretory pathway. Syntaxins as well as other SNARE proteins play important roles during vesicle secretion and fusion. We have compared the secretion of newly synthesised cell-wall polysaccharides to that of secretory marker proteins such as secreted green-fluorescent protein (secGFP) and secreted rat preputial β-glu ...
DOI:
10.1007/s00425-006-0407-9
PubMed:
17039371

50. A comparative analysis of green fluorescent protein and β-glucuronidase protein-encoding genes as a reporter system for studying the temporal expression profiles of promoters

Author:
P. Kavita; Pradeep Kumar Burma
Source:
Journal of biosciences 2008 v.33 no.3 pp. 337-343
ISSN:
0250-5991
Subject:
Arabidopsis thaliana; Nicotiana tabacum; beta-glucuronidase; fluorescence; green fluorescent protein; messenger RNA; reporter genes; transcriptome
Abstract:
... The assessment of activity of promoters has been greatly facilitated by the use of reporter genes. However, the activity as assessed by reporter gene is a reflection of not only promoter strength, but also that of the stability of the mRNA and the protein encoded by the reporter gene. While a stable reporter gene product is an advantage in analysing activities of weak promoters, it becomes a major ...
DOI:
10.1007/s12038-008-0053-4

51. Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light

Author:
Wirth, S.; Segretin, M.E.; Mentaberry, A.; Bravo-Almonacid, F.
Source:
Journal of biotechnology 2006 v.125 no.2 pp. 159-172
ISSN:
0168-1656
Subject:
Nicotiana tabacum; chloroplast DNA; genetic transformation; genetic vectors; gene transfer; transgenes; epidermal growth factor; humans; protein synthesis; recombinant fusion proteins; beta-glucuronidase; light; chloroplasts
DOI:
10.1016/j.jbiotec.2006.02.012

52. Analysis of polarity in the expression from a multifactorial bidirectional promoter designed for high-level expression of transgenes in plants

Author:
Chaturvedi, C.P.; Sawant, S.V.; Kiran, K.; Mehrotra, R.; Lodhi, N.; Ansari, S.A.; Tuli, R.
Source:
Journal of biotechnology 2006 v.123 no.1 pp. 1-12
ISSN:
0168-1656
Subject:
Nicotiana tabacum; transgenic plants; promoter regions; plasmid vectors; gene expression; transgenes; beta-glucuronidase; green fluorescent protein; transcription (genetics); transcriptional activation; salicylic acid; sodium chloride; indole acetic acid
DOI:
10.1016/j.jbiotec.2005.10.014

53. Characterization of FaRB7, a near root-specific gene from strawberry (Fragariaxananassa Duch.) and promoter activity analysis in homologous and heterologous hosts

Author:
Vaughan, Simon P.; James, David J.; Lindsey, Keith; Massiah, Andrea J.
Source:
Journal of experimental botany 2006 v.57 no.14 pp. 3901-3910
ISSN:
0022-0957
Subject:
genetic transformation; strawberries; petioles; genetic resistance; Fragaria ananassa; beta-glucuronidase; transgenic plants; host plants; phylogeny; Nicotiana tabacum; disease resistance; reporter genes; tonoplast; promoter regions; roots; tobacco; plant proteins
Abstract:
... Many insect and fungal pathogens posing agronomically important threats specifically target the roots in strawberry. The use of a root-specific promoter to confer expression of resistance genes in a targeted manner has the potential appreciably to benefit the genetic improvement of commercial strawberry varieties. A novel gene, FaRB7, was isolated from strawberry (Fragariaxananassa Duch.) and foun ...
DOI:
10.1093/jxb/erl185
PubMed:
17032728

54. Colonization by the Arbuscular Mycorrhizal Fungus Glomus versiforme Induces a Defense Response Against the Root-knot Nematode Meloidogyne incognita in the Grapevine (Vitis amurensis Rupr.), Which Includes Transcriptional Activation of the Class III Chitinase Gene VCH3

Author:
Li, Hai-Yan; Yang, Guo-Dong; Shu, Huai-Rui; Yang, Yu-Tao; Ye, Bao-Xing; Nishida, Ikuo; Zheng, Cheng-Chao
Source:
Plant & cell physiology 2006 v.47 no.1 pp. 154-163
ISSN:
0032-0781
Subject:
vesicular arbuscular mycorrhizae; transcriptional activation; plant biochemistry; mycorrhizal fungi; grapes; plant genetics; Glomus versiforme; Meloidogyne incognita; transgenic plants; Vitis amurensis; microbial colonization; resistance mechanisms; Nicotiana tabacum; root-knot nematodes; promoter regions; beta-glucuronidase; tobacco; chitinase
Abstract:
... Inoculation of the grapevine (Vitis amurensis Rupr.) with the arbuscular mycorrhizal (AM) fungus Glomus versiforme significantly increased resistance against the root-knot nematode (RKN) Meloidogyne incognita. Studies using relative quantitative reverse transcription-PCR (RQRT-PCR) analysis of grapevine root inoculation with the AM fungus revealed an up-regulation of VCH3 transcripts. This increas ...
DOI:
10.1093/pcp/pci231
PubMed:
16326755

55. Distinct repressing modules on the distal region of the SBP2 promoter contribute to its vascular tissue-specific expression in different vegetative organs

Author:
Freitas, Rejane L.; Carvalho, Claudine M.; Fietto, Luciano G.; Loureiro, Marcelo E.; Almeida, Andrea M.; Fontes, Elizabeth P. B.
Source:
Plant molecular biology 2007 v.65 no.5 pp. 603-614
ISSN:
0167-4412
Subject:
Glycine max; Nicotiana tabacum; beta-glucuronidase; binding proteins; consensus sequence; gene expression; leaves; phloem; promoter regions; reporter genes; root growth; roots; shoots; stems; sucrose; tobacco; transgenic plants; vascular bundles
Abstract:
... The Glycine max sucrose binding protein (GmSBP2) promoter directs vascular tissue-specific expression of reporter genes in transgenic tobacco. Here we showed that an SBP2-GFP fusion protein under the control of the GmSBP2 promoter accumulates in the vascular tissues of vegetative organs, which is consistent with the proposed involvement of SBP in sucrose transport-dependent physiological processes ...
DOI:
10.1007/s11103-007-9225-0
PubMed:
17710554

56. Downstream promoter sequence of an Indian isolate of Rice tungro bacilliform virus alters tissue-specific expression in host rice and acts differentially in heterologous system

Author:
Mathur, Saloni; Dasgupta, Indranil
Source:
Plant molecular biology 2007 v.65 no.3 pp. 259-275
ISSN:
0167-4412
Subject:
Nicotiana tabacum; Oryza sativa; Rice tungro bacilliform virus; Triticum turgidum subsp. durum; beta-glucuronidase; developmental stages; enhancer elements; gene expression; host specificity; hosts; promoter regions; reporter genes; rice; rice protein; tobacco; transcription (genetics); transgenic plants; wheat; India; Philippines
Abstract:
... An Indian isolate of Rice tungro bacilliform virus from West Bengal (RTBV-WB) showed significant nucleotide differences in its putative promoter region when compared with a previously characterized isolate from Philippines. The transcription start site of RTBV-WB was mapped followed by assessing the activity and tissue-specificity of the full-length (FL) promoter (-231 to +645) and several of its ...
DOI:
10.1007/s11103-007-9214-3
PubMed:
17721744

57. Heat-inducible production of β-glucuronidase in tobacco hairy root cultures

Author:
Kung-Ta Lee; Shih-Cheng Chen; Bor-Luen Chiang; Takashi Yamakawa
Source:
Applied microbiology and biotechnology 2007 v.73 no.5 pp. 1047-1053
ISSN:
0175-7598
Subject:
Nicotiana tabacum; beta-glucuronidase; clones; heat; heat shock proteins; heat stress; messenger RNA; roots; temperature; tobacco; viability
Abstract:
... The production of β-glucuronidase (GUS) driven by the Arabidopsis small heat shock protein 18.2 promoter in liquid cultures of transgenic tobacco (Nicotiana tabacum) hairy roots is reported. Clone GD-3, showing high GUS heat induction and a moderate growth rate, was selected from 436 clones for study. Treatment of GD-3 with heat shock at 36–42°C for 2 h then recovery at 27°C resulted in an increas ...
DOI:
10.1007/s00253-006-0576-2

58. The influence of matrix attachment regions on transgene expression in Arabidopsis thaliana wild type and gene silencing mutants

Author:
Miguel F. C. De Bolle; Katleen M. J. Butaye; Inge J. W. M. Goderis; Piet F. J. Wouters; Anni Jacobs; Stijn L. Delauré; Ann Depicker; Bruno P. A. Cammue
Source:
Plant molecular biology 2007 v.63 no.4 pp. 533-543
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; Nicotiana tabacum; beta-glucuronidase; chickens; gene expression; gene silencing; genetic background; lysozyme; mutants; progeny; tobacco; transgenes; transgenic plants
Abstract:
... Many studies in both animal and plant systems have shown that matrix attachment regions (MARs) can increase the expression of flanking transgenes. However, our previous studies revealed no effect of the chicken lysozyme MARs (chiMARs) on transgene expression in the first generation transgenic Arabidopsis thaliana plants transformed with a β-glucuronidase gene (uidA) unless gene silencing mutants w ...
DOI:
10.1007/s11103-006-9107-x
PubMed:
17136580

59. Abscisic acid-inducible nuclear proteins bind to bipartite promoter elements required for ABA response and embryo-regulated expression of the carrot Dc3 gene

Author:
Chung, H.J.; Fu, H.Y.; Thomas, T.L.
Source:
Planta 2005 v.220 no.3 pp. 424-433
ISSN:
0032-0935
Subject:
plant genetics; transgenic plants; Daucus carota; vegetable crops; signal transduction; Nicotiana tabacum; reporter genes; abscisic acid; carrots; gene expression regulation; promoter regions; beta-glucuronidase; tobacco; plant proteins
Abstract:
... The carrot (Daucus carota L.) lea-class gene Dc3 is expressed in developing seeds and in vegetative tissues subject to drought and treatment with exogenous abscisic acid (ABA). Cis regulatory elements involved in seed-specific expression and in response to ABA were identified in transgenic tobacco (Nicotiana tabacum L.) using beta-glucuronidase (GUS) reporter gene constructs containing a series of ...
DOI:
10.1007/s00425-004-1366-7
PubMed:
15378369

60. Aluminum borate whisker-mediated production of transgenic tobacco plants

Author:
Mizuno, K.; Takahashi, W.; Beppu, T.; Shimada, T.; Tanaka, O.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 163-169
ISSN:
0167-6857
Subject:
Nicotiana tabacum; tobacco; leaves; genetic transformation; callus; gene transfer; aluminum; borate minerals; silicon; carbides; agitation; transgenic plants; histochemistry; beta-glucuronidase; enzyme activity
Abstract:
... An aluminum borate whiskers-mediated transformation system for calluses of tobacco (Nicotiana tabacum, cv. SR-1) has been developed. A total of 50 small pieces of calluses were vigorously agitated in a liquid medium containing aluminum borate whiskers, pBI221 plasmid carrying the beta-glucuronidase (GUS) gene, and pBI222 plasmid carrying the hygromycin phosphotransferase (HPT) gene. After treatmen ...

61. Assessment of the Effectiveness of a Nuclear-Launched TMV-Based Replicon as a Tool for Foreign Gene Expression in Plants in Comparison to Direct Gene Expression from a Nuclear Promoter

Author:
Man, M.; Epel, B.L.
Source:
Transgenic research 2006 v.15 no.1 pp. 107-113
ISSN:
0962-8819
Subject:
coat proteins; genetic transformation; Tobacco mosaic virus; replicon; complementary DNA; transgenic plants; Agrobacterium radiobacter; gene expression; Nicotiana tabacum; reporter genes; promoter regions; beta-glucuronidase; tobacco
DOI:
10.1007/s11248-005-2942-8
PubMed:
16475015

62. Characterization of a Strong, Constitutive Mung Bean (Vigna radiata L.) Promoter with a Complex Mode of Regulation in planta

Author:
Cazzonelli, C.I.; McCallum, E.J.; Lee, R.; Botella, J.R.
Source:
Transgenic research 2005 v.14 no.6 pp. 941-967
ISSN:
0962-8819
Subject:
Vigna radiata; mung beans; promoter regions; 1-aminocyclopropane-1-carboxylate synthase; genetic transformation; gene transfer; transgenic plants; Arabidopsis thaliana; Nicotiana tabacum; gene expression; reporter genes; beta-glucuronidase; luciferase; messenger RNA
DOI:
10.1007/s11248-005-2539-2
PubMed:
16315097

63. Development of Novel Types of Plastid Transformation Vectors and Evaluation of Factors Controlling Expression

Author:
Herz, S.; Fussi, M.; Steiger, S.; Koop, H.U.
Source:
Transgenic research 2005 v.14 no.6 pp. 969-982
ISSN:
0962-8819
Subject:
Nicotiana tabacum; tobacco; genetic transformation; chloroplast DNA; plasmid vectors; operon; reporter genes; beta-glucuronidase; gene expression; transgenic plants
DOI:
10.1007/s11248-005-2542-7
PubMed:
16315098

64. Effects of codon modification on human BMP2 gene expression in tobacco plants

Author:
Guangli Suo; Bing Chen; Jingyu Zhang; Ziyuan Duan; Zhengquan He; Wei Yao; Chaoyin Yue; Jianwu Dai
Source:
Plant cell reports 2006 v.25 no.7 pp. 689-697
ISSN:
0721-7714
Subject:
genetic transformation; humans; transgenic plants; reverse transcriptase polymerase chain reaction; Western blotting; codons; Nicotiana tabacum; genomics; gene expression regulation; genetic vectors; beta-glucuronidase; tobacco
Abstract:
... Bone morphogenetic protein 2 (BMP2) has great potential in therapeutic applications. We are working on generating transgenic plants as a bioreactor to produce BMP2. We have studied the effects of codon optimization on the expression of human BMP2 (hBMP2) in tobacco plants. Three modified hBMP2 genes were transformed into tobacco under the control of either cauliflower mosaic virus 35S (CaMV35S) pr ...
DOI:
10.1007/s00299-006-0133-6
PubMed:
16491379

65. Expression of a ferredoxin-dependent glutamate synthase gene in mesophyll and vascular cells and functions of the enzyme in ammonium assimilation in Nicotiana tabacum (L.)

Author:
Feraud, M.; Masclaux-Daubresse, C.; Ferrario-Mery, S.; Pageau, K.; Lelandais, M.; Ziegler, C.; Leboeuf, E.; Jouglet, T.; Viret, L.; Spampinato, A.
Source:
Planta 2005 v.222 no.4 pp. 667-677
ISSN:
0032-0935
Subject:
plant biochemistry; glutamate synthase (ferredoxin); plant proteins; assimilation (physiology); recombinant fusion proteins; Nicotiana tabacum; mesophyll; gene expression regulation; Arabidopsis thaliana; ammonium compounds; phloem; plant physiology; tobacco; plant genetics; transgenes; nitrogen; transgenic plants; physiological transport; biochemical pathways; nucleotide sequences; reporter genes; beta-glucuronidase
Abstract:
... GLU1 encodes the major ferredoxin-dependent glutamate synthase (Fd-GOGAT, EC 1.4.7.1) in Arabidopsis thaliana (ecotype Columbia). With the aim of providing clues on the role of Fd-GOGAT, we analyzed the expression of Fd-GOGAT in tobacco (Nicotiana tabacum L. cv. Xanthi). The 5' flanking element of GLU1 directed the expression of the uidA reporter gene in the palisade and spongy parenchyma of mesop ...
DOI:
10.1007/s00425-005-0013-2
PubMed:
16034598

66. Expression of a polyubiquitin promoter isolated from Gladiolus

Author:
Joung, Y.H.; Kamo, K.
Source:
Plant cell reports 2006 v.25 no.10 pp. 1081-1088
ISSN:
0721-7714
Subject:
Gladiolus; ubiquitin; promoter regions; sequence analysis; introns; gene expression regulation; genetic transformation; Zantedeschia; Freesia; Rosa; Nicotiana tabacum; Arabidopsis thaliana; transgenic plants; beta-glucuronidase; luciferase
Abstract:
... A polyubiquitin promoter (GUBQ1) including its 5'UTR and intron was isolated from the floral monocot Gladiolus because high levels of expression could not be obtained using publicly available promoters isolated from either cereals or dicots. Sequencing of the promoter revealed highly conserved 5' and 3' intron splicing sites for the 1.234 kb intron. The coding sequence of the first two ubiquitin g ...
Handle:
10113/29222
DOI:
10.1007/s00299-006-0185-7
PubMed:
16761144

67. Expression of three Arabidopsis cytokinin oxidase/dehydrogenase promoter::GUS chimeric constructs in tobacco: response to developmental and biotic factors

Author:
Galis, I.; Bilyeu, K.D.; Godinho, M.J.G.; Jameson, P.E.
Source:
Plant growth regulation 2005 v.45 no.3 pp. 173-182
ISSN:
0167-6903
Subject:
plant pathogenic bacteria; developmental stages; chimerism; genes; promoter regions; roots; gene expression; Nicotiana tabacum; disease resistance; leaves; Arabidopsis thaliana; plant development; Rhodococcus fascians; pathogenicity; enzyme activity; tobacco; gene transfer; plant breeding; transgenic plants; trichomes; oxidoreductases; cytokinins; plant morphology; beta-glucuronidase
Abstract:
... Expression patterns of three Arabidopsis thaliana cytokinin oxidase/dehydrogenase promoter::GUS reporter fusions were investigated in tobacco plants. While cytokinin oxidase/dehydrogenase promoter 2 showed no expression in tobacco, the cytokinin oxidase/dehydrogenase promoters 3 and 4 were active in various tissues throughout development of the tobacco. Recently, the 1452 bp promoter region of AtC ...

68. Molecular characterization of mature pollen-specific genes encoding novel small cysteine-rich proteins in rice (Oryza sativa L.)

Author:
Park, J.; Hakozaki, H.; Endo, M.; Takada, Y.; Ito, H.; Uchida, M.; Okabe, T.; Watanabe, M.
Source:
Plant cell reports 2006 v.25 no.5 pp. 466-474
ISSN:
0721-7714
Subject:
Oryza sativa; rice; pollen; pollen tubes; plant proteins; multigene family; promoter regions; Nicotiana tabacum; tobacco; transgenic plants; beta-glucuronidase; gene expression regulation; genomics; amino acid sequences
Abstract:
... In our previous cDNA microarray analysis, we identified 53 mature anther-specific genes, whose function was unknown, in rice. We reanalyzed these genes from the viewpoint of the specific amino acid motif. Out of 53 genes, three genes, Os-26, Os-32, and Os-169 (renamed as OsSCP1, OsSCP2, and OsSCP3), encoded cysteine-rich motif (Cys-X3-Cys-X13-Cys-X3-Cys), indicating that they were novel small cyst ...
DOI:
10.1007/s00299-005-0077-2
PubMed:
16397782

69. A Novel Plant Cysteine Protease Has a Dual Function as a Regulator of 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene Expression

Author:
Matarasso, Noa; Schuster, Silvia; Avni, Adi
Source:
plant cell 2005 v.17 no.4 pp. 1205-1216
ISSN:
1040-4651
Subject:
tomatoes; transcription factors; Solanum lycopersicum var. lycopersicum; beta-glucuronidase; transgenic plants; transcription (genetics); gene expression; binding proteins; Nicotiana tabacum; reporter genes; 1-aminocyclopropane-1-carboxylate synthase; endo-1,4-beta-xylanase; gene expression regulation; promoter regions; binding capacity; tobacco; cysteine proteinases
Abstract:
... The hormone ethylene influences plant growth, development, and some defense responses. The fungal elicitor Ethylene-Inducing Xylanase (EIX) elicits ethylene biosynthesis in tomato (Lycopersicon esculentum) and tobacco (Nicotiana tabacum) leaves by induction of 1-aminocyclopropane-1-caboxylic acid synthase (Acs) gene expression. A minimal promoter element in the LeAcs2 gene required for EIX respons ...
DOI:
10.1105/tpc.105.030775
PubMed:
15749766
PubMed Central:
PMC1087997

70. Overexpression of carnation S-adenosylmethionine decarboxylase gene generates a broad-spectrum tolerance to abiotic stresses in transgenic tobacco plants

Author:
Wi, S.J.; Kim, W.T.; Park, K.Y.
Source:
Plant cell reports 2006 v.25 no.10 pp. 1111-1121
ISSN:
0721-7714
Subject:
Dianthus caryophyllus; methionine adenosyltransferase; beta-glucuronidase; promoter regions; transgenes; complementary DNA; gene overexpression; Nicotiana tabacum; tobacco; transgenic plants; stress tolerance; polyamines; signal transduction; photosynthesis; antioxidants; ascorbate peroxidase; superoxide dismutase; glutathione transferase; messenger RNA; gene expression regulation; abiotic stress; oxidative stress
Abstract:
... Polyamines (PAs), such as putrescine, spermidine, and spermine, are present in all living organism and implicate in a wide range of cellular physiological processes. We have used transgenic technology in an attempt to evaluate their potential for mitigating the adverse effects of several abiotic stresses in plants. Sense construct of full-length cDNA for S-adenosylmethionine decarboxylase (SAMDC), ...
DOI:
10.1007/s00299-006-0160-3
PubMed:
16642382

71. Taproot promoters cause tissue specific gene expression within the storage root of sugar beet

Author:
Heiko Oltmanns; Dorothee U. Kloos; Waltraud Brieß; Maike Pflugmacher; Dietmar J. Stahl; Reinhard Hehl
Source:
Planta 2006 v.224 no.3 pp. 485-495
ISSN:
0032-0935
Subject:
Mesembryanthemum crystallinum; Beta vulgaris subsp. vulgaris; sugar beet; transgenic plants; root systems; plant proteins; promoter regions; reporter genes; beta-glucuronidase; gene expression regulation; tobacco; Nicotiana tabacum; hypocotyls
Abstract:
... The storage root (taproot) of sugar beet (Beta vulgaris L.) originates from hypocotyl and primary root and contains many different tissues such as central xylem, primary and secondary cambium, secondary xylem and phloem, and parenchyma. It was the aim of this work to characterize the promoters of three taproot-expressed genes with respect to their tissue specificity. To investigate this, promoters ...
DOI:
10.1007/s00425-006-0230-3
PubMed:
16482437

72. Two cis-acting regulatory elements are involved in the sucrose-inducible expression of the sporamin gene promoter from sweet potato in transgenic tobacco

Author:
Morikami, A.; Matsunaga, R.; Tanaka, Y.; Suzuki, S.; Mano, S.; Nakamura, K.
Source:
Molecular genetics and genomics 2005 v.272 no.6 pp. 690-699
ISSN:
1617-4615
Subject:
Ipomoea batatas; sweet potatoes; promoter regions; sporamin; recombinant DNA; reporter genes; beta-glucuronidase; gene transfer; gene expression; transgenic plants; Nicotiana tabacum; tobacco; gene expression regulation; sucrose; leaves
DOI:
10.1007/s00438-004-1100-y
PubMed:
15654621

73. Ubiquitous presence of β-glucuronidase (GUS) in plants and its regulation in some model plants

Author:
Sudan, C.; Prakash, S.; Bhomkar, P.; Jain, S.; Bhalla-Sarin, N.
Source:
Planta 2006 v.224 no.4 pp. 853-864
ISSN:
0032-0935
Subject:
Arabidopsis thaliana; Oryza sativa; Nicotiana tabacum; Zea mays; Cajanus cajan; pigeon peas; plant growth; beta-glucuronidase; enzyme activity; tissue distribution; pH; benzyladenine; gibberellic acid
Abstract:
... The enzyme β-glucuronidase (GUS) is well characterized in animals and microbes. However, this enzyme is not well studied in plants and is widely assumed to be absent in them. In this study we document the ubiquitous presence of GUS in the model plants Arabidopsis thaliana, Oryza sativa, Nicotiana tabacum and Zea mays and record its expression pattern. The pH of the assay buffer was found to be cri ...
DOI:
10.1007/s00425-006-0276-2

74. Activation of defense pathways: synergism between reactive oxygen species and salicylic acid and consideration of field applicability

Author:
Blec, K.A.; Yang, K.Y.; Anderson, A.J.
Source:
European journal of plant pathology 2004 v.110 no.2 pp. 203-212
ISSN:
0929-1873
Subject:
application timing; cell death; application methods; salicylic acid; promoter regions; peracetic acid; systemic acquired resistance; hydrogen peroxide; gene expression; Nicotiana tabacum; disease resistance; synergism; resistance mechanisms; enzyme activity; tobacco; plant genetics; Pseudomonas syringae pv. tabaci; transgenic plants; dosage; beta-glucuronidase; synergists
Abstract:
... Treatment of tobacco with a mixture containing reactive oxygen species (ROS) and salicylic acid (SA) provided greater protection of tobacco against infection by Pseudomonas syringae pv. tabaci than either treatment alone. Synergism in expression from the promoter of the defense gene PR-1a was also observed. Although the ROS hydrogen peroxide and peracetic acid were poor inducers alone, they enhanc ...

75. Analysis of Arabidopsis PsbQA gene expression in transgenic tobacco reveals differential role of its promoter and transcribed region in organ-specific and light-mediated regulation

Author:
Gaur, T.; Tyagi, A.K.
Source:
Transgenic research 2004 v.13 no.2 pp. 97-108
ISSN:
0962-8819
Subject:
Arabidopsis thaliana; Nicotiana tabacum; tobacco; transgenic plants; plant proteins; oxygen evolving complex; promoter regions; reporter genes; beta-glucuronidase; enzyme activity; structural genes; transcription (genetics); messenger RNA; gene expression regulation; light; exons; leaves; inheritance (genetics)
DOI:
10.1023/B:TRAG.0000026050.23122.29
PubMed:
15198198

76. Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum

Author:
Koscianska, E.; Kalantidis, K.; Wypijewski, K.; Sadowski, J.; Tabler, M.
Source:
Plant molecular biology 2005 v.59 no.4 pp. 647-661
ISSN:
0167-4412
Subject:
genetic transformation; RNA interference; green fluorescent protein; transgenic plants; Nicotiana benthamiana; Agrobacterium radiobacter; transfer DNA; Nicotiana tabacum; reporter genes; double-stranded RNA; beta-glucuronidase; small interfering RNA
PubMed:
16244913

77. The AoPR10 promoter and certain endogenous PR10 genes respond to oxidative signals in Arabidopsis

Author:
Mur, L.A.J.; Sturgess, F.J.; Farrell, G.G.; Draper, J.
Source:
Molecular plant pathology 2004 v.5 no.5 pp. 435-451
ISSN:
1464-6722
Subject:
plant pathogenic bacteria; sequence alignment; reactive oxygen species; signal transduction; salicylic acid; plant damage; promoter regions; Asparagus officinalis; systemic acquired resistance; Nicotiana tabacum; enzyme inhibition; gene expression regulation; Arabidopsis thaliana; superoxide dismutase; Pseudomonas syringae pv. tomato; amino acid sequences; pathogenesis-related proteins; hypersensitive response; tobacco; genetic transformation; transgenic plants; reporter genes; NADH or NADPH oxidoreductases; beta-glucuronidase
DOI:
10.1111/j.1364-3703.2004.00244.x
PubMed:
20565619

78. Bi-directional duplex promoters with duplicated enhancers significantly increase transgene expression in grape and tobacco

Author:
Li, Z.T.; Jayasankar, S.; Gray, D.J.
Source:
Transgenic research 2004 v.13 no.2 pp. 143-154
ISSN:
0962-8819
Subject:
plant viruses; genetic transformation; enhancer elements; Cassava vein mosaic virus; green fluorescent protein; grapes; transgenes; kanamycin kinase; transgenic plants; Agrobacterium radiobacter; plasmid vectors; transcription (genetics); Nicotiana tabacum; Cauliflower mosaic virus; Vitis vinifera; gene expression regulation; promoter regions; beta-glucuronidase; tobacco
DOI:
10.1023/B:TRAG.0000026074.11859.77
PubMed:
15198202

79. Efficient developmental mis-targeting by the sporamin NTPP vacuolar signal to plastids in young leaves of sugarcane and Arabidopsis

Author:
Gnansambandam, A.; Birch, R.G.
Source:
Plant cell reports 2004 v.23 no.7 pp. 435-447
ISSN:
0721-7714
Subject:
guard cells; sporamin; luciferase; Solanum tuberosum; vacuoles; cytosol; potatoes; sugarcane; callus culture; Saccharum officinarum; recombinant fusion proteins; tissue distribution; Nicotiana tabacum; signal peptide; mesophyll; leaves; somatic embryos; Arabidopsis thaliana; cell nucleus; tobacco; genetic transformation; green fluorescent protein; transgenes; transgenic plants; cultured cells; gene fusion; reporter genes; nucleotide sequences; beta-glucuronidase; chloroplasts; chitinase
DOI:
10.1007/s00299-004-0860-5
PubMed:
15372194

80. Functional characterization of tobacco transcription factor TGA2.1

Author:
Kegler, C.; Lenk, I.; Krawczyk, S.; Scholz, R.; Gatz, C.
Source:
Plant molecular biology 2004 v.55 no.2 pp. 153-164
ISSN:
0167-4412
Subject:
transcription factors; transcriptional activation; messenger RNA; transgenic plants; transcription (genetics); auxins; Nicotiana tabacum; salicylic acid; reporter genes; mutants; pathogenesis-related proteins; promoter regions; beta-glucuronidase; tobacco
PubMed:
15604672

81. Gene transfer to plants by diverse species of bacteria

Author:
Broothaerts, W.; Mitchell, H.J.; Weir, B.; Kaines, S.; Smith, L.M.A.; Yang, W.; Mayer, J.E.; Roa-Rodriguez, C.; Jefferson, R.A.
Source:
Nature 2005 v.433 no.7026 pp. 629-633
ISSN:
0028-0836
Subject:
Arabidopsis thaliana; genetic transformation; gene transfer; rice; Oryza sativa; transgenic plants; Ensifer meliloti; plasmids; Nicotiana tabacum; reporter genes; Rhizobium; Mesorhizobium loti; beta-glucuronidase; tobacco
Abstract:
... Agrobacterium is widely considered to be the only bacterial genus capable of transferring genes to plants. When suitably modified, Agrobacterium has become the most effective vector for gene transfer in plant biotechnology. However, the complexity of the patent landscape has created both real and perceived obstacles to the effective use of this technology for agricultural improvements by many publ ...
DOI:
10.1038/nature03309

82. Identification of cis-elements for ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase

Author:
Rawat, R.; Xu, Z.F.; Yao, K.M.; Chye, M.L.
Source:
Plant molecular biology 2005 v.57 no.5 pp. 629-643
ISSN:
0167-4412
Subject:
eggplants; ethylene; Solanum melongena; transcription factors; transgenic plants; luciferase; binding sites; reporter genes; circadian rhythm; Nicotiana tabacum; gene expression regulation; promoter regions; beta-glucuronidase; cysteine proteinases
Abstract:
... We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions and by in vitro binding assays. Analysis of transgenic tobacco transformed with SmC ...
PubMed:
15988560

83. In planta measurements of oxidative bursts elicited by avirulent and virulent bacterial pathogens suggests that H2O2 is insufficient to elicit cell death in tobacco

Author:
Mur, L.A.J.; Kenton, P.; Draper, J.
Source:
Plant, cell and environment 2005 v.28 no.4 pp. 548-561
ISSN:
0140-7791
Subject:
plant pathogenic bacteria; oxidative stress; avirulent strains; catalase; transgenic plants; hydrogen peroxide; beta-glucuronidase; amino acid sequences; Nicotiana tabacum; Pseudomonas syringae pv. syringae; nucleotide sequences; tobacco; gene expression regulation; hypersensitive response; apoptosis; Pseudomonas syringae pv. tabaci
Abstract:
... A rapid and localized programmed cell death - the hypersensitive response (HR) - is a widely utilized plant resistance mechanism against pathogens. Studies have implicated H2O2 generation as a key elicitory mechanism in the HR. The causal relationship between the kinetics of the in planta oxidative burst, the HR and certain defence gene expression was examined. H2O2 generation following challenge ...
DOI:
10.1111/j.1365-3040.2005.01301.x

84. Inducible excision of selectable marker gene from transgenic plants by the Cre/lox site-specific recombination system

Author:
Wang, Y.; Chen, B.; Hu, Y.; Li, J.; Lin, Z.
Source:
Transgenic research 2005 v.14 no.5 pp. 605-614
ISSN:
0962-8819
Subject:
transgenic plants; bacteriophages; heat stress; Nicotiana tabacum; reporter genes; phosphotransferases (kinases); heritability; genetic vectors; beta-glucuronidase; tobacco; genetic recombination
DOI:
10.1007/s11248-005-0884-9
PubMed:
16245151

85. Role of Hsp17.4-CII as coregulator and cytoplasmic retention factor of tomato heat stress transcription factor HsfA2

Author:
Port, M.; Tripp, J.; Zielinski, D.; Weber, C.; Heerklotz, D.; Winkelhaus, S.; Bublak, D.; Scharf, K.D.
Source:
Plant physiology 2004 v.135 no.3 pp. 1457-1470
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; plant proteins; transcription factors; transcription (genetics); heat shock proteins; protein-protein interactions; Nicotiana tabacum; tobacco; transgenic plants; protoplasts; protein aggregates; beta-glucuronidase; reporter genes; two hybrid system techniques; fluorescent antibody technique; amino acid sequences; nucleotide sequences
Abstract:
... HsfA2 is a heat stress (hs)-induced Hsf in peruvian tomato (Lycopersicon peruvianum) and the cultivated form Lycopersicon esculentum. Due to the high activator potential and the continued accumulation during repeated cycles of heat stress and recovery, HsfA2 becomes a dominant Hsf in thermotolerant cells. The formation of heterooligomeric complexes with HsfA1 leads to nuclear retention and enhance ...
DOI:
10.1104/pp.104.042820
PubMed:
15247379
PubMed Central:
PMC519062

86. Signal peptide-dependent targeting of a rice alpha-amylase and cargo proteins to plastids and extracellular compartments of plant cells

Author:
Chen, M.H.; Huang, L.F.; Li, H.M.; Chen, Y.R.; Yu, S.M.
Source:
Plant physiology 2004 v.135 no.3 pp. 1367-1377
ISSN:
0032-0889
Subject:
amyloplasts; rice; green fluorescent protein; grain crops; transgenes; cell walls; Oryza sativa; transgenic plants; microsomes; protein transport; signal peptide; Nicotiana tabacum; reporter genes; tobacco; gene expression regulation; alpha-amylase; beta-glucuronidase; chloroplasts; plant proteins
Abstract:
... alpha-Amylases are important enzymes for starch degradation in plants. However, it has been a long-running debate as to whether alpha-amylases are localized in plastids where starch is stored. To study the subcellular localization of alpha-amylases in plant cells, a rice (Oryza sativa) alpha-amylase, alpha Amy3, with or without its own signal peptide (SP) was expressed in transgenic tobacco (Nicot ...
DOI:
10.1104/pp.104.042184
PubMed:
15235120
PubMed Central:
PMC519054

87. Stable transformation of petunia plastids

Author:
Zubko, M.K.; Zubko, E.I.; van Zuilen, K.; Meyer, P.; Day, A.
Source:
Transgenic research 2004 v.13 no.6 pp. 523-530
ISSN:
0962-8819
Subject:
genetic transformation; spectinomycin; transgenes; Petunia hybrida; biolistics; antibiotic resistance; transgenic plants; chloroplast DNA; Nicotiana tabacum; leaves; reporter genes; inheritance (genetics); beta-glucuronidase; chloroplasts
DOI:
10.1007/s11248-004-2374-x
PubMed:
15672833

88. Surrogate Splicing for Functional Analysis of Sesquiterpene Synthase Genes

Author:
Wu, Shuiqin; Schoenbeck, Mark A.; Greenhagen, Bryan T.; Takahashi, Shunji; Lee, Sungbeom; Coates, Robert M.; Chappell, Joseph
Source:
Plant physiology 2005 v.138 no.3 pp. 1322-1333
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; plant genetics; complementary DNA; Petunia hybrida; transgenic plants; RNA splicing; gene expression; Nicotiana tabacum; reporter genes; alkyl (aryl) transferases; introns; beta-glucuronidase; tobacco
Abstract:
... A method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns is described. The approach utilizes Agrobacterium-mediated transient expression coupled with a reverse transcription-polydeoxyribonucleotide chain reaction assay to facilitate expression cloning of processed transcripts. Subsequent expression of intronless cDNAs in a suitable prokaryotic host pr ...
DOI:
10.1104/pp.105.059386
PubMed:
15965019
PubMed Central:
PMC1176406

89. Tomato heat stress transcription factor HsfB1 represents a novel type of general transcription coactivator with a histone-like motif interacting with the plant CREB binding protein ortholog HAC1

Author:
Bharti, K.; Koskull-Doring, P. von; Bharti, S.; Kumar, P.; Tintschl-Korbitzer, A.; Treuter, E.; Nover, L.
Source:
plant cell 2004 v.16 no.6 pp. 1521-1535
ISSN:
1040-4651
Subject:
Solanum peruvianum; tomatoes; Nicotiana tabacum; Nicotiana benthamiana; tobacco; Arabidopsis thaliana; transcription factors; protein binding; protein-protein interactions; histones; amino acid sequences; binding sites; binding capacity; promoter regions; Cauliflower mosaic virus; transcription (genetics); transcriptional activation; transgenic plants; protoplasts; reporter genes; beta-glucuronidase; luciferase; heat stress
Abstract:
... In contrast with the class A heat stress transcription factors (HSFs) of plants, a considerable number of HSFs assigned to classes B and C have no evident function as transcription activators on their own. However, in the following article, we provide evidence that tomato (Lycopersicon peruvianum) HsfB1 represents a novel type of coactivator cooperating with class A HSFs (e.g., with tomato HsfA1). ...
DOI:
10.1105/tpc.019927
PubMed:
15131252
PubMed Central:
PMC490043

90. The alc-GR System. A Modified alc Gene Switch Designed for Use in Plant Tissue Culture

Author:
Roberts, Gethin R.; Garoosi, G. Ali; Koroleva, Olga; Ito, Masaki; Laufs, Patrick; Leader, David J.; Caddick, Mark X.; Doonan, John H.; Tomsett, A. Brian
Source:
Plant physiology 2005 v.138 no.3 pp. 1259-1267
ISSN:
0032-0889
Subject:
Nicotiana tabacum; tobacco; transgenic plants; cell suspension culture; Solanum lycopersicum var. lycopersicum; tomatoes; plant proteins; transcription factors; beta-glucuronidase; green fluorescent protein; reporter genes; gene expression regulation; tissue culture; plant genetics
Abstract:
... The ALCR/alcA (alc) two-component, ethanol-inducible gene expression system provides stringent control of transgene expression in genetically modified plants. ALCR is an ethanol-activated transcription factor that can drive expression from the ALCR-responsive promoter (alcA). However, the alc system has been shown to have constitutive expression when used in plant callus or cell suspension culture ...
DOI:
10.1104/pp.105.059659
PubMed:
16010000
PubMed Central:
PMC1176399

91. The promoter of the Arabidopsis thaliana Cel1 endo-1,4-beta glucanase gene is differentially expressed in plant feeding cells induced by root-knot and cyst nematodes

Author:
Mitchum, M.G.; Sukno, S.; Wang, X.; Shani, Z.; Tsabary, G.; Shoseyov, O.; Davis, E.L.
Source:
Molecular plant pathology 2004 v.5 no.3 pp. 175-181
ISSN:
1464-6722
Subject:
developmental stages; roots; promoter regions; plant proteins; tissue distribution; Globodera tabacum; Heterodera schachtii; Nicotiana tabacum; gene expression regulation; Arabidopsis thaliana; giant cells; messenger RNA; root-knot nematodes; tobacco; transgenes; transgenic plants; Meloidogyne incognita; reporter genes; endo-1,4-beta-glucanase; beta-glucuronidase
DOI:
10.1111/j.1364-3703.2004.00216.x
PubMed:
20565607

92. A quick and efficient system for antibiotic-free expression of heterologous genes in tobacco roots

Author:
Komarnytsky, S.; Gaume, A.; Garvey, A.; Borisjuk, N.; Raskin, I.
Source:
Plant cell reports 2004 v.22 no.10 pp. 765-773
ISSN:
0721-7714
Subject:
genetic transformation; transgenes; genetic vectors; antibiotics; transgenic plants; Agrobacterium radiobacter; Rhizobium rhizogenes; gene expression; genetic markers; Nicotiana tabacum; bacterial proteins; micropropagation; in vitro regeneration; roots; beta-glucuronidase; tobacco
Abstract:
... Requirement for antibiotic-resistance selection markers and difficulty in identifying transgenes with the highest expression levels remain the major obstacles for rapid production of recombinant proteins in plants. An alternative approach to producing transgenic plants free of antibiotic-resistance markers is the phenotypic-based selection with root-proliferation genes (rol genes) of Agrobacterium ...
DOI:
10.1007/s00299-004-0761-7
PubMed:
14770265

93. The rice metallothionein gene promoter does not direct foreign gene expression in seed endosperm

Author:
Fukuzawa, H.; Yu, L.H.; Umeda-Hara, C.; Tagawa, M.; Uchimiya, H.
Source:
Plant cell reports 2004 v.23 no.4 pp. 231-235
ISSN:
0721-7714
Subject:
rice; transgenes; Oryza sativa; transgenic plants; gene expression; metallothionein; endosperm; Nicotiana tabacum; gene expression regulation; promoter regions; beta-glucuronidase; tobacco
DOI:
10.1007/s00299-004-0813-z
PubMed:
15278421

94. Activity of elements from the tobacco cryptic promoter, tCUP, in conifer tissues

Author:
Tian, L.; Wu, K.; Levasseur, C.; Ouellet, T.; Foster, E.; Latoszek-Green, M.; Sibbald, S.; Miki, B.; Seguin, A.; Brown, D.C.W.
Source:
In vitro cellular & developmental biology 2003 v.39 no.2 pp. 193-202
ISSN:
1054-5476
Subject:
genetic transformation; gene transfer; enhancer elements; Larix; transgenic plants; luciferase; transcription (genetics); Picea glauca; Nicotiana tabacum; reporter genes; Pinus taeda; Pseudotsuga menziesii; promoter regions; beta-glucuronidase
DOI:
10.1079/IVP2002365

95. Cell specific, cross-species expression of myrosinases in Brassica napus, Arabidopsis thaliana and Nicotiana tabacum

Author:
Thangstad, O.P.; Gilde, B.; Chadchawan, S.; Seem, M.; Husebye, H.; Bradley, D.; Bones, A.M.
Source:
Plant molecular biology 2004 v.54 no.4 pp. 597-611
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; guard cells; recombinant DNA; transgenic plants; embryo (plant); gene expression; phloem; Nicotiana tabacum; Brassica napus; thioglucosidase; reporter genes; tobacco; enzyme activity; promoter regions; beta-glucuronidase; rapeseed
Abstract:
... A prototypical characteristic of the Brassicaceae is the presence of the myrosinase-glucosinolate system. Myrosinase, the only known S-glycosidase in plants, degrades glucosinolates, thereby initiating the formation of isothiocyanates, nitriles and other reactive products with biological activities. We have used myrosinase gene promoters from Brassica napus and Arabidopsis thaliana fused to the β- ...
PubMed:
15316292

96. Cestrum yellow leaf curling virus (CmYLCV) promoter: a new strong constitutive promoter for heterologous gene expression in a wide variety of crops

Author:
Stavolone, L.; Kononova, M.; Pauli, S.; Ragozzino, A.; Haan, P. de; Milligan, S.; Lawton, K.; Hohn, T.
Source:
Plant molecular biology 2003 v.53 no.5 pp. 663-673
ISSN:
0167-4412
Subject:
plant viruses; Caulimoviridae; promoter regions; reporter genes; beta-glucuronidase; chloramphenicol acetyltransferase; gene transfer; genetic transformation; gene expression; transgenic plants; Arabidopsis thaliana; Nicotiana tabacum; tobacco; Solanum lycopersicum var. lycopersicum; tomatoes; Zea mays; corn; Oryza sativa; rice
Abstract:
... Appropriately regulated gene expression requires a suitable promoter. A number of promoters have been isolated and shown to be functional in plants, but only a few of them activate transcription of transgenes at high levels constitutively. We report here the cloning and characterization of a novel, constitutively expressed promoter isolated from Cestrum yellow leaf curling virus (CmYLCV), a double ...
PubMed:
15010605

97. Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors

Author:
Leelavathi, S.; Reedy, V.S.
Source:
Molecular breeding 2003 v.11 no.1 pp. 49-58
ISSN:
1380-3743
Subject:
Nicotiana tabacum; tobacco; transgenic plants; genetic transformation; gene transfer; genes; chloroplast DNA; recombinant fusion proteins; beta-glucuronidase; interferons; histidine; gene expression; protein synthesis; genetic engineering; chloroplasts
Abstract:
... High level expression and efficient recovery of recombinant protein are two main critical factors that determine the use of transgenic plants as natural bioreactors to produce foreign proteins for industrial applications. We demonstrate here the potential of a new strategy involving chloroplast transformation, GUS-fusions and affinity-tag based chromatography to overexpress and purify a human ther ...

98. Differences in the processing of DNA ends in Arabidopsis thaliana and tobacco: possible implications for genome evolution

Author:
Orel, N.; Puchta, H.
Source:
Plant molecular biology 2003 v.51 no.4 pp. 523-531
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; genetic transformation; biolistics; transgenic plants; plasmids; gene expression; Nicotiana tabacum; leaves; reporter genes; beta-glucuronidase; tobacco
Abstract:
... Surprising species-specific differences in non-homologous end-joining (NHEJ) of genomic double-strand breaks (DSBs) have been reported for the two dicotyledonous plants Arabidopsis thaliana and Nicotiana tabacum. In Arabidopsis deletions were, on average, larger than in tobacco and not associated with insertions. To establish the molecular basis of the phenomenon we analysed the fate of free DNA e ...
PubMed:
12650618

99. Efficient male germ line transformation for transgenic tobacco production without selection

Author:
Aziz, N.; Machray, G.C.
Source:
Plant molecular biology 2003 v.51 no.2 pp. 203-211
ISSN:
0167-4412
Subject:
fertilization (reproduction); Nicotiana tabacum; tobacco; genetic transformation; pollen; biolistics; reporter genes; beta-glucuronidase; gene expression; cell culture; pollination; hygromycin B; drug resistance
Abstract:
... Microspores at late uninucleate/early binucleate stages were isolated from flower buds of tobacco (Nicotiana tabacum L.) and in vitro culture methods optimised for their maturation to fully functional viable pollen which, after application to the stigma of emasculated plants in situ, led to the generation of large numbers of seed. Efficient protocols were established for the biolistic introduction ...
PubMed:
12602879

100. Expression of the peroxidase gene promoter (Shpx6b) from Stylosanthes humilis in transgenic plants during insect attack

Author:
Perera, M.R.; Jones, M.G.K.
Source:
Entomologia experimentalis et applicata 2004 v.111 no.3 pp. 165-171
ISSN:
0013-8703
Subject:
Phthorimaea operculella; pest resistance; promoter regions; Myzus persicae; tissue distribution; gene expression; Nicotiana tabacum; mechanical damage; peroxidase; insect pests; methyl jasmonate; induced resistance; tobacco; green fluorescent protein; phytophagous insects; transgenic plants; Stylosanthes humilis; reporter genes; gene induction; beta-glucuronidase
DOI:
10.1111/j.0013-8703.2004.00170.x