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You searched for: Subject beta-glucuronidase Remove constraint Subject: beta-glucuronidase Subject term Triticum aestivum Remove constraint Subject term: Triticum aestivum
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1. pEmu: an improved promoter for gene expression in cereal cells

Author:
Last, D.I.; Brettell, R.I.S.; Chamberlain, D.A.; Chaudhury, A.M.; Larkin, P.J.; Marsh, E.L.; Peacock, W.J.; Dennis, E.S.
Source:
Theoretical and applied genetics 1991 v.81 no.5 pp. 581-588
ISSN:
0040-5752
Subject:
Triticum aestivum; Zea mays; Oryza sativa; Triticum monococcum; Lolium multiflorum; Nicotiana plumbaginifolia; genetic transformation; gene expression; protoplasts; direct DNA uptake; electroporation; recombinant DNA; reporter genes; beta-glucuronidase; promoter regions
Abstract:
... A recombinant promoter, pEmu, has been constructed to give a high level of gene expression in monocots. It is based on a truncated maize Adh1 promoter, with multiple copies of the Anaerobic Responsive Element from the maize Adh1 gene and ocs-elements from the octopine synthase gene of Agrobacterium tumefaciens. The pEmu promoter was one of 12 different promoter constructs that were linked to the b ...
DOI:
10.1007/BF00226722
PubMed:
24221371

2. Factors affecting transient gene expression in protoplasts isolated from very slowly growing embryogenic callus cultures of wheat (Triticum aestivum L.)

Author:
Zaghmout, O.M.F.; Trolinder, N.L.
Source:
Theoretical and applied genetics 1993 v.86 no.6 pp. 721-730
ISSN:
0040-5752
Subject:
alcohol dehydrogenase; protoplasts; genetic transformation; Tobacco mosaic virus; recombinant DNA; transgenic plants; enzyme activity; plasmid vectors; gene expression; histochemistry; Triticum aestivum; reporter genes; Cauliflower mosaic virus; Vitis vinifera; embryogenesis; promoter regions; beta-glucuronidase; callus; electroporation
Abstract:
... Protoplasts isolated from embryogenic ('Mustang' and 'Chinese Spring') and non-embryogenic ('Mit') calli of wheat (Triticum aestivum L.) genotypes transiently expressed beta-glucuronidase (GUS) activity when electroporated with a plasmid containing the GUS gene and driven by an enhanced 35S promoter and a TMV leader sequence. Conditions for the maximum expression of GUS activity were: electroporat ...
DOI:
10.1007/BF00222662
PubMed:
24193782

3. Spatial-temporal colonization patterns of Azospirillum brasilense on the wheat root surface and expression of the bacterial nifH gene during association

Author:
Broek, A.V.; Michiels, J.; Gool, A. van.; Vanderleyden, J.
Source:
Molecular plant-microbe interactions 1993 v.6 no.5 pp. 592-600
ISSN:
0894-0282
Subject:
temporal variation; promoter regions; nitrogen fixation; recombinant DNA; spatial distribution; Azospirillum brasilense; histochemistry; Triticum aestivum; adhesion; acetylene; reporter genes; nitrogenase; roots; beta-glucuronidase
DOI:
10.1094/MPMI-6-592

4. Fertile transgenic wheat from microprojectile bombardment of scutellar tissue

Author:
Becker, D.; Brettschneider, R.; Lorz, H.
Source:
plant journal 1994 v.5 no.2 pp. 299-307
ISSN:
0960-7412
Subject:
genetic transformation; transgenic plants; embryo (plant); Oryza sativa; plasmids; gene expression; Triticum aestivum; genes; artificial regeneration; actin; enzyme activity; beta-glucuronidase; genetic markers; tissue culture
DOI:
10.1046/j.1365-313X.1994.05020299.x
PubMed:
8148881

5. Transformation of protoplasts and intact cells from slowly growing embryogenic callus of wheat (Triticum aestivum L.)

Author:
Zaghmout, O.M.F.
Source:
Theoretical and applied genetics 1994 v.89 no.5 pp. 577-582
ISSN:
0040-5752
Subject:
protoplasts; genetic transformation; Agrobacterium radiobacter; direct DNA uptake; gene expression; polyethylene glycol; Triticum aestivum; reporter genes; embryogenesis; beta-glucuronidase; callus; electroporation
Abstract:
... A procedure for culturing protoplasts from slowly growing embryogenic calli of wheat was developed. The procedure was dependent on the ability to isolate large numbers of culturable protoplasts from slowly growing embryogenic callus. Approximately 68% of the isolated protoplasts divided, and 22% formed colonies; of the latter, 67% continued to proliferate. Plating efficiency was reduced when proto ...
DOI:
10.1007/BF00222451
PubMed:
24177933

6. Adenine methylation at dam sites increases transient gene expression in plant cells

Author:
Graham, M.W.; Larkin, P.J.
Source:
Transgenic research 1995 v.4 no.5 pp. 324-331
ISSN:
0962-8819
Subject:
Triticum aestivum; Nicotiana plumbaginifolia; Trifolium subterraneum; DNA methylation; adenine; Escherichia coli; gene transfer; genetic transformation; gene expression; reporter genes; beta-glucuronidase; recombinant DNA; protoplasts; electroporation; promoter regions
DOI:
10.1007/BF01972529
PubMed:
8589735

7. Molecular characterization of the fate of transgenes in transformed wheat (Triticum aestivum L.)

Author:
Srivastava, V.; Vasil, V.; Vasil, I.K.
Source:
Theoretical and applied genetics 1996 v.92 no.8 pp. 1031-1037
ISSN:
0040-5752
Subject:
Southern blotting; gene transfer; DNA methylation; phosphinothricin acetyltransferase; transgenic plants; gene expression; Triticum aestivum; reporter genes; inheritance (genetics); genetic variation; enzyme activity; histochemistry; beta-glucuronidase
Abstract:
... Molecular analysis of the transgenes bar and gus was carried out over successive generations in six independent transgenic lines of wheat, until the plants attained homozygosity. Data on expression and integration of the transgenes is presented. Five of the lines were found to be stably transformed, duly transferring the transgenes to the next generation. The copy number of the transgenes varied f ...
DOI:
10.1007/BF00224045
PubMed:
24166632

8. Cooperative of two distinct cis-acting elements is necessary for the S phase-specific activation of the wheat histone H3 promoter

Author:
Ohtsubo, N.; Nakayama, T.; Kaya, H.; Terada, R.; Shimamoto, K.; Meshi, T.; Iwabuchi, M.
Source:
plant journal 1997 v.11 no.6 pp. 1219-1225
ISSN:
0960-7412
Subject:
promoter regions; messenger RNA; histones; Oryza sativa; transgenic plants; plasmid vectors; mutation; gene expression; Triticum aestivum; interphase; beta-glucuronidase
Abstract:
... We have previously shown that the proximal promoter region (-185 to +57) of the wheat histone H3 gene (TH012) is sufficient for regulating S phase-expression of a reporter GUS gene. To define the cis-acting element(s) responsible for S phase-specific expression, GUS fusion genes under the control of wild-type or variously mutated H3 promoters were stably introduced into cultured rice Oc cells and ...
DOI:
10.1046/j.1365-313X.1997.11061219.x

9. Genetic transformation of wheat mediated by Agrobacterium tumefaciens

Author:
Cheng, M.; Fry, J.E.; Pang, S.Z.; Zhou, H.P.; Hironaka, C.M.; Duncan, D.R.; Conner, T.W.; Wan, Y.C.
Source:
Plant physiology 1997 v.115 no.3 pp. 971-980
ISSN:
0032-0889
Subject:
developmental stages; neomycin; inheritance (genetics); genetic markers; callus; Agrobacterium radiobacter; explants; gene expression; Triticum aestivum; plant development; phosphotransferases (phosphomutases); enzyme activity; histochemistry; genetic transformation; gene transfer; transgenic plants; embryo (plant); rapid methods; plant morphology; genetic vectors; beta-glucuronidase
Abstract:
... A rapid Agrobacterium tumefaciens-mediated transformation system for wheat was developed using freshly isolated immature embryos, precultured immature embryos, and embryogenic calli as explants. The explants were inoculated with a disarmed A. tumefaciens strain C58 (ABI) harboring the binary vector pMON18365 containing the beta-glucuronidase gene with an intron, and a selectable marker, the neomyc ...

10. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

11. Endophytic establishment of diazotrophic bacteria in auxin-induced tumors of cereal crops

Author:
Christiansen-Weniger, C.
Source:
Critical reviews in plant sciences 1998 v.17 no.1 pp. 55-76
ISSN:
0735-2689
Subject:
Triticum aestivum; Zea mays; Oryza sativa; root nodules; auxins; hypocotyls; neoplasms; root meristems; Azospirillum; Rhizobium; nitrogen-fixing bacteria; cortex; infection; cytoplasm; endophytes; nitrogenase; enzyme activity; carbon; histology; beta-glucuronidase; genetic markers; nitrogen fixation; photosynthesis; translocation (plant physiology); Azorhizobium caulinodans; ammonium compounds
DOI:
10.1080/07352689891304168

12. A transient assay for evaluating promoters in wheat endosperm tissue

Author:
Clarke, B.C.; Appels, R.
Source:
Genome = 1998 v.41 no.6 pp. 865-871
ISSN:
0831-2796
Subject:
genetic transformation; biolistics; chromosomes; assays; prolamins; gene expression; endosperm; Triticum aestivum; reporter genes; enzyme activity; promoter regions; beta-glucuronidase
Abstract:
... A transient assay was developed for the evaluation of promoter sequences in wheat endosperm tissue. A deletion series from an omega-secalin gene promoter, located on chromosome 1RS.1DL of specific wheat lines, were translationally fused to a uidA reporter gene. These promoters were evaluated for expression in wheat endosperm tissue after integration of the DNA into the cell using microprojectile b ...
DOI:
10.1139/gen-41-6-865
PubMed:
9924795

13. Analysis of particle bombardment parameters to optimise DNA delivery into wheat tissues

Author:
Rasco-Gaunt, S.; Riley, A.; Barcelo, P.; Lazzeri, P.A.
Source:
Plant cell reports 1999 v.19 no.2 pp. 118-127
ISSN:
0721-7714
Subject:
calcium chloride; genetic transformation; inflorescences; inorganic ions; biolistics; transgenic plants; calcium; plasmid vectors; spermidine; particle size; gene expression; Triticum aestivum; reporter genes; beta-glucuronidase; tissue culture
Abstract:
... The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to wheat (Triticum aestivum L.) scutellum and inflorescence tissue. The main factors studied were the DNA/gold precipitation process, bombardment parameters and tissue culture variables. Efficiency of DNA (uidA gene) delivery was assessed by scoring transient GUS expression in bombarde ...
DOI:
10.1007/s002990050721

14. Transient gene expression and influence of promoters on foreign gene expression in Arabidopsis thaliana

Author:
Gandhi, R.; Maheshwari, S.C.; Khurana, P.
Source:
In vitro cellular & developmental biology 1999 v.35 no.3 pp. 232-237
ISSN:
1054-5476
Subject:
Arabidopsis thaliana; gene expression; transgenic plants; mesophyll; protoplasts; polyethylene glycol; beta-glucuronidase; plasmid vectors; DNA; magnesium; cations; enzyme activity; reporter genes; Oryza sativa; Triticum aestivum; Brassica; promoter regions; inorganic ions; calcium
DOI:
10.1007/s11627-999-0084-z

15. Variations in the maize Ac transposase transcript level and the Ds excision frequency in transgenic wheat callus lines

Author:
Takumi, S.; Murai, K.; Mori, N.; Nakamura, C.
Source:
Genome = 1999 v.42 no.6 pp. 1234-1241
ISSN:
0831-2796
Subject:
Triticum aestivum; Zea mays; messenger RNA; transgenic plants; callus; transposons; beta-glucuronidase; reporter genes; biolistics; gene expression; nucleotidyltransferases; plasmids; promoter regions
Abstract:
... To investigate the excision of a maize transposable element in wheat cells, plasmid DNAs containing a Dissociation (Ds) element located between a rice actin 1 gene promoter and a beta-glucuronidase (GUS) gene (gus) were introduced into wheat callus lines by microprojectile bombardment, and transient GUS expression was assayed. The gus-expressing cells after Ds excision were detected only when the ...
DOI:
10.1139/g99-077

16. A transient assay system for the functional assessment of defense-related genes in wheat

Author:
Schweizer, P.; Pokorny, J.; Abderhalden, O.; Dudler, R.
Source:
Molecular plant-microbe interactions 1999 v.12 no.8 pp. 647-654
ISSN:
0894-0282
Subject:
biolistics; phenotype; molecular weight; assays; transgenic plants; plasmid vectors; plant proteins; Blumeria graminis f. sp. tritici; gene expression; fungi; Triticum aestivum; reporter genes; cell wall components; enzyme activity; histochemistry; beta-glucuronidase; leaves; defense mechanisms
Abstract:
... The generation and characterization of transgenic wheat plants is a tedious and time-consuming process that limits the number of putatively important transgenes that can be tested. We therefore established a transient assay system based on wheat leaves to study the effect of transiently expressed genes on the interaction with the wheat powdery mildew fungus Erysiphe (syn. Blumeria) graminis f. sp. ...
DOI:
10.1094/MPMI.1999.12.8.647

17. Elucidation of Septoria tritici x wheat interactions using GUS-expressing isolates

Author:
Pnini-Cohen, S.; Zilberstein, A.; Schuster, S.; Sharon, A.; Eyal, Z.
Source:
Phytopathology 2000 v.90 no.3 pp. 297-304
ISSN:
0031-949X
Subject:
Triticum aestivum; Mycosphaerella graminicola; plant pathogenic fungi; host-parasite relationships; virulence; beta-glucuronidase; enzyme activity; diagnostic techniques; genetic transformation; gene transfer; genetic markers; reporter genes; gene expression
Abstract:
... Isolate ISR398 of Septoria tritici (which produces none to few pycnidia on the wheat cv. Seri 82 and high coverage on cv. Shafir) and isolate ISR8036 (which is virulent on both cultivars) were genetically cotransformed using the selectable marker gene hph, which confers resistance to hygromycin B (hygB), and the reporter gene uidA, encoding beta-glucuronidase (GUS). Most of the genetically transfo ...
DOI:
10.1094/PHYTO.2000.90.3.297
PubMed:
18944623

18. Evaluation of Dasypyrum villosum populations for resistance to cereal eyespot and stripe rust pathogens

Author:
Yildirim, A.; Jones, S.S.; Murray, T.D.; Line, R.F.
Source:
Plant disease 2000 v.84 no.1 pp. 40-44
ISSN:
0191-2917
Subject:
germplasm; rust diseases; population; genetic resistance; strain differences; Oculimacula yallundae; chromosome mapping; plant genetic resources; disease resistance; Dasypyrum villosum; Triticum aestivum; provenance; chromosome translocation; Puccinia striiformis; beta-glucuronidase; Italy; Greece; Yugoslavia; Turkey (country)
Abstract:
... Resistance to Pseudocercosporella herpotrichoides (cause of eyespot) and Puccinia striiformis (cause of stripe rust) was evaluated in a germ plasm collection of Dasypyrum villosum (syn. Haynaldia villosa) and a set of disomic addition lines, a substitution, and a translocation line of D. villosum chromosomes in a wheat background. Three races of P. striiformis and a beta-glucuronidase-transformed ...
Handle:
10113/48436
DOI:
10.1094/PDIS.2000.84.1.40

19. Marker gene expression driven by the maize ubiquitin promoter in transgenic wheat

Author:
Rooke, L.; Byrne, D.; Salgueiro, S.
Source:
Annals of applied biology 2000 v.136 no.2 pp. 167-172
ISSN:
0003-4746
Subject:
genetic transformation; promoter regions; Zea mays; transgenic plants; gene expression; Triticum aestivum; genotype; ubiquitin; enzyme activity; histochemistry; beta-glucuronidase; genetic markers
DOI:
10.1111/j.1744-7348.2000.tb00022.x

20. A matrix attachment region is located upstream from the high-molecular-weight glutenin gene Bx7 in wheat (Triticum aestivum L.)

Author:
Rampitsch, C.; Jordan, M.C.; Cloutier, S.
Source:
Genome 2000 v.43 no.3 pp. 483-486
ISSN:
0831-2796
Subject:
glutenins; alleles; loci; biolistics; transgenic plants; polymerase chain reaction; codons; gene expression; chromosome mapping; Triticum aestivum; reporter genes; DNA-binding domains; molecular weight; promoter regions; beta-glucuronidase; leaves
Abstract:
... A 2.2-kb nucleotide sequence rich in AT, located upstream from Bx7 allele of the high-molecular-weight glutenin Glu-B1 locus in wheat (Triticum aestivum cv. Glenlea) was cloned following amplification by PCR. The 5' region of this sequence contains motifs typically found in matrix attachment regions (MARs) in other plants. We have shown that part of the 2.2-kb DNA binds to wheat nuclear matrix (NM ...
DOI:
10.1139/g00-011
PubMed:
10902712

21. A plant virus vector for systemic expression of foreign genes in cereals

Author:
Choi, I.R.; Stenger, D.C.; Morris, T.J.; French, R.
Source:
plant journal 2000 v.23 no.4 pp. 547-555
ISSN:
0960-7412
Subject:
Wheat streak mosaic virus; seedlings; roots; interspecific variation; Hordeum vulgare; open reading frames; gene expression; Triticum aestivum; virulence; leaves; infection; coat proteins; Zea mays; polymerase chain reaction; histochemistry; genetic transformation; Avena sativa; reporter genes; genetic vectors; beta-glucuronidase
DOI:
10.1046/j.1365-313x.2000.00820.x
PubMed:
10972881

22. Infection of winter wheat by a beta-glucuronidase-transformed isolate of Cephalosporium gramineum

Author:
Douhan, G.W.; Murray, T.D.
Source:
Phytopathology 2001 v.91 no.3 pp. 232-239
ISSN:
0031-949X
Subject:
developmental stages; seasonal variation; plant pathogenic fungi; genes; appressoria; roots; stems; gene expression; Triticum aestivum; disease resistance; pathotypes; infection; cortex; cultivars; pathogenicity; winter wheat; enzyme activity; ultrastructure; genetic transformation; gene transfer; Cephalosporium; transgenic plants; genetic variation; beta-glucuronidase
Abstract:
... Field-grown winter wheat was inoculated with a beta-glucuronidase-transformed isolate of Cephalosporium gramineum in two field seasons to elucidate the mode of infection in resistant and susceptible cultivars. Colonization of viable root epidermis and cortical cells occurred as soon as 15 days postinoculation and the pathogen was found in the vascular tissues by 20 days postinoculation, well befor ...
DOI:
10.1094/PHYTO.2001.91.3.232
PubMed:
18943341

23. 5' deletion of a gbss1 promoter region from wheat leads to changes in tissue and developmental specificities

Author:
Kluth, A.; Sprunck, S.; Becker, D.; Lorz, H.; Lutticke, S.
Source:
Plant molecular biology 2002 v.49 no.6 pp. 669-682
ISSN:
0167-4412
Subject:
hexosyltransferases; filling period; pericarp; phenotype; transgenic plants; endosperm; Triticum aestivum; mutants; reporter genes; histochemistry; enzyme activity; promoter regions; beta-glucuronidase
Abstract:
... Expression of granule-bound starch synthase 1 (GBSSI) in wheat is restricted to the grain filling process. In order to identify promoter regions which are involved in transcriptional control of the observed expression pattern, we isolated about 8 kb of a wheat gbss1-upstream region. Within this sequence several putative cis-acting elements were identified. In addition, an untranslated leader regio ...
PubMed:
12081374

24. High transformation frequencies obtained from a commercial wheat (Triticum aestivum L. cv. 'Combi') by microbombardment of immature embryos followed by GFP screening combined with PPT selection

Author:
Huber, M.; Hahn, R.; Hess, D.
Source:
Molecular breeding 2002 v.10 no.1/2 pp. 19-30
ISSN:
1380-3743
Subject:
genetic transformation; green fluorescent protein; biolistics; animal proteins; embryo (plant); shoots; gene expression; Scyphozoa; Triticum aestivum; glufosinate; reporter genes; herbicide resistance; beta-glucuronidase; acyltransferases
Abstract:
... Improved gene transfer techniques are necessary to obtain adequate numbers of stable transgenic wheat plants needed for practical purposes. Considering that wheat transformation is genotype-dependent, we used cv. Combi in all experiments, which had been selected from agronomically important German spring wheat cultivars because of its high transformation ability. In most wheat gene transfer attemp ...

25. Manipulation of discriminatory T-DNA delivery by Agrobacterium into cells of immature embryos of barley and wheat

Author:
Ke, X.Y.; McCormac, A.C.; Harvey, A.; Lonsdale, D.; Chen, D.F.; Elliott, M.C.
Source:
Euphytica 2002 v.126 no.3 pp. 333-343
ISSN:
0014-2336
Subject:
genetic transformation; application rate; Hordeum vulgare; Agrobacterium radiobacter; embryo (plant); plasmid vectors; gene expression; Triticum aestivum; reporter genes; embryogenesis; embryo culture; culture media; beta-glucuronidase

26. Powerful effect of an atypical bifactorial endosperm box from wheat HMWG-Dx5 promoter in maize endosperm

Author:
Norre, F.; Peyrot, C.; Garcia, C.; Rance, I.; Drevet, J.; Theisen, M.; Gruber, V.
Source:
Plant molecular biology 2002 v.50 no.4/5 pp. 699-712
ISSN:
0167-4412
Subject:
Triticum aestivum; Zea mays; endosperm; glutenins; gene expression; transcription factors; beta-glucuronidase; reporter genes; transgenic plants
Abstract:
... The proximal region of the high-molecular-weight glutenin promoter of the Dx5 gene (PrHMWG-Dx5) carries an atypical bifactorial endosperm box containing two cis-acting elements, namely a G-box like motif followed by a prolamin-box motif (Pb1). Transient expression assays in maize endosperm indicate that a promoter fragment containing at least the G-box like element is necessary and sufficient for ...

27. Starch synthesis in potato tubers transformed with wheat genes for ADPglucose pyrophosphorylase

Author:
Vardy, K.A.; Emes, M.J.; Burrell, M.M.
Source:
Functional plant biology 2002 v.29 no.8 pp. 975-985
ISSN:
1445-4408
Subject:
genetic transformation; carbohydrate metabolism; immunoblotting; isotope labeling; glucose; transgenic plants; Agrobacterium radiobacter; Solanum tuberosum; starch; Triticum aestivum; nucleotide sequences; reporter genes; phosphotransferases (kinases); enzyme activity; stable isotopes; beta-glucuronidase

28. Recovery and characterization of transgenic plants from two spring wheat cultivars with low embryogenesis efficiencies by the bombardment of isolated scutella

Author:
Li, B.; Leung, N.; Caswell, K.; Chibbar, R.N.
Source:
In vitro cellular & developmental biology 2003 v.39 no.1 pp. 12-19
ISSN:
1054-5476
Subject:
genetic transformation; cultivars; biolistics; transgenes; transgenic plants; cotyledons; gene expression; spring wheat; Triticum aestivum; reporter genes; gene segregation; beta-glucuronidase
DOI:
10.1079/IVP2002372

29. Stable wheat transformation obtained without selectable markers

Author:
Permingeat, H.R.; Alvarez, M.L.; Cervigni, G.D.L.; Ravizzini, R.A.; Vallejos, R.H.
Source:
Plant molecular biology 2003 v.52 no.2 pp. 415-419
ISSN:
0167-4412
Subject:
Triticum aestivum; wheat; transgenic plants; genetic transformation; biolistics; reporter genes; beta-glucuronidase; hygromycin B; antibiotic resistance; gene segregation
Abstract:
... Transgenic wheat plants without the selectable marker gene were obtained either in the presence or in the absence of selective pressure during the transformation protocol. When using hygromycin as selective agent in a co-transformation experiment involving a mixture of plasmids pGL2, containing the hpt gene, and pAI1 Gus, containing the uidA gene, 3 out of 19 transgenic wheat plants had the uidA g ...
PubMed:
12856946

30. Tissue specificity of the sugarcane bacilliform virus promoter in oat, barley and wheat

Author:
Al-Saady, N.A.; Torbert, K.A.; Smith, L.; Makarevitch, I.; Baldridge, G.; Zeyen, R.J.; Muehlbauer, G.J.; Olszewski, N.E.; Somers, D.A.
Source:
Molecular breeding 2004 v.14 no.3 pp. 331-338
ISSN:
1380-3743
Subject:
Avena sativa; oats; Hordeum vulgare; barley; Triticum aestivum; wheat; genetic transformation; biolistics; transgenic plants; promoter regions; Sugarcane bacilliform virus; reporter genes; beta-glucuronidase; gene expression; endosperm; leaves; flowers; embryo (plant)
Abstract:
... The tissue-specificity of the sugarcane bacilliform virus (SCBV) promoter was investigated in oat, barley, and wheat to determine whether its expression pattern in one species was predictive of promoter specificity in the other closely related Gramineae species. Progeny of transgenic plants produced using constructs containing the SCBV promoter driving gusA were sampled at different stages of plan ...

31. Calluses initiated from thin mature embryo fragments are suitable targets for wheat transformation as assessed by long-term GUS expression studies

Author:
Delporte, F.; Li, S.; Jacquemin, J.M.
Source:
Plant cell, tissue, and organ culture 2005 v.80 no.2 pp. 139-149
ISSN:
0167-6857
Subject:
genetic transformation; dose response; biolistics; transgenic plants; embryo (plant); Agrobacterium radiobacter; explants; wheat; gene expression; Triticum aestivum; 2,4-D; micropropagation; enzyme activity; in vitro regeneration; culture media; beta-glucuronidase; callus
Abstract:
... Microprojectile- or Agrobacterium-mediated DNA delivery into calluses initiated from immature embryos has proven to be effective in transforming wheat. Yet, obtaining a large number of high quality immature embryos throughout the year is a laborious and delicate process. To circumvent these limitations, we propose an alternative technique applying the particle bombardment technology to calluses de ...

32. Evidence for non-proteinaceous inhibitor(s) of beta-glucuronidase in wheat (Triticum aestivum L.) leaf and root tissues

Author:
Bahieldin, A.; Eissa, H.F.; Mahfouz, H.T.; Dyer, W.E.; Madkour, M.A.; Qu, R.
Source:
Plant cell, tissue, and organ culture 2005 v.82 no.1 pp. 11-17
ISSN:
0167-6857
Subject:
Triticum aestivum; wheat; leaves; roots; beta-glucuronidase; enzyme activity; enzyme inhibitors; genetic transformation; transgenic plants; transgenes; gene expression; cultivars; genetic variation
Abstract:
... The GUS gene of E. coli, encoding beta-glucuronidase, has been widely used as a reporter gene in plant transformation. However, beta-glucuronidase activity in transgenic wheat leaf or root tissue is rarely observed or reported. To address this question, we investigated three wheat lines transformed with the GUS reporter gene. We found all three lines expressed GUS mRNA as well as beta-glucuronidas ...

33. The evolution of the Abscisic acid-response in land plants: comparative analysis of Group 1 LEA gene expression in moss and cereals

Author:
Kamisugi, Y.; Cuming, A.C.
Source:
Plant molecular biology 2005 v.59 no.5 pp. 723-737
ISSN:
0167-4412
Subject:
osmotic pressure; wheat; mosses and liverworts; promoter regions; plant proteins; barley; Hordeum vulgare; gene expression; Triticum aestivum; gene expression regulation; introns; messenger RNA; amino acid sequences; abiotic stress; Physcomitrella patens; transgenic plants; reporter genes; abscisic acid; beta-glucuronidase
PubMed:
16270226

34. An internal RNA element in the P3 cistron of Wheat streak mosaic virus revealed by synonymous mutations that affect both movement and replication

Author:
Choi, I.R.; Horken, K.M.; Stenger, D.C.; French, R.
Source:
Journal of general virology 2005 v.86 pp. 2605-2614
ISSN:
0022-1317
Subject:
Wheat streak mosaic virus; plant diseases and disorders; beta-glucuronidase; mutation; wheat; gene expression; mutants; reporter genes; Triticum aestivum; RNA; virus replication
Abstract:
... Multiple synonymous substitution mutations in the Wheat streak mosaic virus P3 cistron did not affect translation in vitro but rendered the virus incapable of systemic infection. Multiple synonymous substitutions in the cylindrical inclusion cistron did not alter infectivity or in vitro translation. To assess replication and movement phenotypes, P3 mutations were placed in context with a GUS repor ...
Handle:
10113/40538

35. A model wheat cultivar for transformation to improve resistance to Fusarium Head Blight

Author:
Mackintosh, C.A.; Garvin, D.F.; Radmer, L.E.; Heinen, S.J.; Muehlbauer, G.J.
Source:
Plant cell reports 2006 v.25 no.4 pp. 313-319
ISSN:
0721-7714
Subject:
quantitative trait loci; Fusarium graminearum; cultivars; blight; transgenes; phosphinothricin acetyltransferase; fungal diseases of plants; spring wheat; Triticum aestivum; disease resistance; plant pathogenic fungi; beta-glucuronidase
Abstract:
... Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a major disease problem in wheat (Triticum aestivum). Genetic engineering holds significant potential to enhance FHB resistance in wheat. Due to the requirement of screening for FHB resistance on flowers at anthesis, the number of screens carried out in a year is limited. Our objective was to evaluate the feasibility of using ...
Handle:
10113/30209
DOI:
10.1007/s00299-005-0059-4
PubMed:
16252090

36. The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene family: comparative promoter activity of six TaLtp genes in transgenic rice

Author:
Boutrot, Freddy; Meynard, Donaldo; Guiderdoni, Emmanuel; Joudrier, Philippe; Gautier, Marie-Françoise
Source:
Planta 2007 v.225 no.4 pp. 843-862
ISSN:
0032-0935
Subject:
Oryza sativa; Triticum aestivum; beta-glucuronidase; family support; flowers; leaves; lipids; multigene family; phylogeny; polymerase chain reaction; proteins; reporter genes; rice; stems; transgenic plants; vascular tissues; wheat
Abstract:
... Plant non-specific lipid transfer proteins (nsLTPs) are encoded by a multigene family and support physiological functions, which remain unclear. We adapted an efficient ligation-mediated polymerase chain reaction (LM-PCR) procedure that enabled isolation of 22 novel Triticum aestivum nsLtp (TaLtp) genes encoding types 1 and 2 nsLTPs. A phylogenetic tree clustered the wheat nsLTPs into ten subfamil ...
DOI:
10.1007/s00425-006-0397-7
PubMed:
16983534

37. The maize activator/dissociation system is functional in hexaploid wheat through successive generations

Author:
Pastori, G.M.; Huttly, A.; West, J.; Sparks, C.; Pieters, A.; Luna, C.M.; Jones, H.D.; Foyer, C.H.
Source:
Functional plant biology 2007 v.34 no.9 pp. 835-843
ISSN:
1445-4408
Subject:
genetic transformation; Zea mays; beta-glucuronidase; transgenic plants; polymerase chain reaction; corn; wheat; biochemical pathways; transposition (genetics); Triticum aestivum; genes; enzyme activity; hexaploidy; genetic vectors; transposons
DOI:
10.1071/FP07112

38. Isolation of two storage protein promoters from Hordeum chilense and characterization of their expression patterns in transgenic wheat

Author:
Pistón, Fernando; León, Elena; Lazzeri, Paul A.; Barro, Francisco
Source:
Euphytica 2008 v.162 no.3 pp. 371-379
ISSN:
0014-2336
Subject:
Triticum aestivum; wheat; transgenic plants; gene transfer; gene expression; Hordeum chilense; promoter regions; storage proteins; hordein; reporter genes; beta-glucuronidase; seeds; messenger RNA
Abstract:
... Many plant genetic engineering applications require spatial expression of genes which in turn depends upon the availability of tissue and organ specific promoters. Wild plant species related to cultivated crops are an important source of genetic variability, not only for trait genes but also for regulatory sequences such as promoters. In the present work two new storage protein promoters cloned fr ...
DOI:
10.1007/s10681-007-9530-3

39. Isolation and heterologous transformation analysis of a pollen-specific promoter from wheat (Triticum aestivum L.)

Author:
Chen, Ling; Tu, Zhiming; Hussain, Javeed; Cong, Ling; Yan, Yinjun; Jin, Lian; Yang, Guangxiao; He, Guangyuan
Source:
Molecular biology reports 2010 v.37 no.2 pp. 737-744
ISSN:
0301-4851
Subject:
Triticum aestivum; beta-glucuronidase; gene expression; genes; pollen; sequence analysis; tobacco; wheat
Abstract:
... The promoter of a pollen-specific gene TaPSG719 was isolated from wheat (Triticum aestivum L.) by inverse-PCR (IPCR). Sequence analysis revealed that the promoter contains two cis-acting elements (AGAAA and GTGA) known to confer anther/pollen-specific gene expression which suggests that the promoter of TaPSG719 gene is a pollen-specific one. To ascertain the regulatory function of TaPSG719 promote ...
DOI:
10.1007/s11033-009-9582-7
PubMed:
19562512

40. Function of the wheat TaSIP gene in enhancing drought and salt tolerance in transgenic Arabidopsis and rice

Author:
Hao-Yue Du; Yin-Zhu Shen; Zhan-Jing Huang
Source:
Plant molecular biology 2013 v.81 no.4-5 pp. 417-429
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; Oryza sativa; RNA interference; Triticum aestivum; abscisic acid; beta-glucuronidase; cell membranes; drought; drought tolerance; environmental factors; exons; gene induction; gene overexpression; membrane proteins; microarray technology; mutants; polymerase chain reaction; promoter regions; quantitative analysis; reporter genes; rice; salt stress; salt tolerance; sodium; sodium chloride; start codon; stress tolerance; transgenic plants; water stress; wheat
Abstract:
... Microarray analysis of a salt-tolerant wheat mutant identified a gene of unknown function that was induced by exposure to high levels of salt and subsequently denoted TaSIP (Triticum aestivum salt-induced protein). Quantitative PCR analysis revealed that TaSIP expression was induced not only by salt, but also by drought, abscisic acid (ABA), and other environmental stress factors. Transgenic rice ...
DOI:
10.1007/s11103-013-0011-x
PubMed:
23400831

41. Isolation and Characterization of an Endosperm-Specific Promoter from Wheat (Triticum aestivum L.)

Author:
Fei Song; Cui-Ju Cui; Ling Chen; Yang-Liu Sun; Fei-Fei Wang; Javeed Hussain; Yin Li; Chen Wang; Cheng Wang; Ming-Jie Chen; Yue-Sheng Wang; Guang-Xiao Yang; Guang-Yuan He
Source:
Zeitschrift für Naturforschung 2014 v.67 no.11-12 pp. 611-619
ISSN:
1865-7125
Subject:
sequence analysis; quantitative analysis; staining; transgenic plants; prolamins; nucleotides; wheat; gene expression; endosperm; genes; Triticum aestivum; new family; promoter regions; beta-glucuronidase; tobacco
Abstract:
... Genes coding for avenin-like proteins (ALP) represent a new family of wheat storage protein genes. To find a wheat endosperm-specific promoter, a 1644-bp fragment upstream of the ALP type-B gene (GenBank accession number JN622144) was isolated. The important promoter elements of the ALP type-B gene were ascertained through sequence analysis which revealed that this fragment contains the TATA and C ...
DOI:
10.1515/znc-2012-11-1212

42. Manipulation of monoubiquitin improves chilling tolerance in transgenic tobacco (Nicotiana tabacum)

Author:
Yanan Feng; Meng Zhang; Qifang Guo; Guokun Wang; Jiangfeng Gong; Ying Xu; Wei Wang
Source:
Plant physiology and biochemistry 2014 v.75 pp. 138-144
ISSN:
0981-9428
Subject:
electrolytes; reactive oxygen species; wheat; genes; sugars; malondialdehyde; proline; vector control; solutes; Triticum aestivum; Nicotiana tabacum; leaves; gene overexpression; ubiquitin; enzyme activity; tobacco; proteasome endopeptidase complex; wilting; transgenic plants; cold stress; photosystem II; cold tolerance; beta-glucuronidase
Abstract:
... Ubiquitin (Ub) is a multifunctional protein that mainly functions to tag proteins for selective degradation by the 26S proteasome. We cloned an Ub gene TaUb2 from wheat (Triticum aestivum L.) previously. To study the function of TaUB2 in chilling stress, sense and antisense Ub transgenic tobacco plants (Nicotiana tabacum L.), as well as wild type (WT) and vector control β-glucuronidase (T-GUS) pla ...
DOI:
10.1016/j.plaphy.2013.11.003

43. Wheat TaSP gene improves salt tolerance in transgenic Arabidopsis thaliana

Author:
Xiaoli Ma; Weina Cui; Wenji Liang; Zhanjing Huang
Source:
Plant physiology and biochemistry 2015 v.97 pp. 187-195
ISSN:
0981-9428
Subject:
Arabidopsis thaliana; Triticum aestivum; abscisic acid; beta-glucuronidase; cell membranes; cold; excretion; gene overexpression; genes; genetic databases; heat; mutants; polyethylene glycol; quantitative polymerase chain reaction; response elements; salicylic acid; salt stress; salt tolerance; sodium; transgenic plants; wheat
Abstract:
... A novel salt-induced gene with unknown functions was cloned through analysis of gene expression profile of a salt-tolerant wheat mutant RH8706-49 under salt stress. The gene was named Triticum aestivum salt-related protein (TaSP) and deposited in GenBank (Accession No. KF307326). Quantitative polymerase chain reaction (qPCR) results showed that TaSP expression was induced under salt, abscisic acid ...
DOI:
10.1016/j.plaphy.2015.10.010

44. Functional study of a salt‐inducible TaSR gene in Triticum aestivum

Author:
Xiao‐Li Ma; Wei‐Na Cui; Qian Zhao; Jing Zhao; Xiao‐Na Hou; Dong‐Yan Li; Zhao‐Liang Chen; Yin‐Zhu Shen; Zhan‐Jing Huang
Source:
Physiologia plantarum 2016 v.156 no.1 pp. 40-53
ISSN:
0031-9317
Subject:
RNA interference; rice; Oryza sativa; wheat; cell membranes; phosphorylation; fluorescence; two hybrid system techniques; gene expression; Triticum aestivum; sodium; start codon; screening; tissues; leaves; Arabidopsis; quantitative analysis; calcium; potassium; salt stress; quantitative polymerase chain reaction; salt tolerance; genetic databases; mutants; beta-glucuronidase
Abstract:
... The gene expression chip of a salt‐tolerant wheat mutant under salt stress was used to clone a salt‐induced gene with unknown functions. This gene was designated as TaSR (Triticum aestivum salt‐response gene) and submitted to GenBank under accession number EF580107. Quantitative polymerase chain reaction (PCR) analysis showed that gene expression was induced by salt stress. Arabidopsis and rice (O ...
DOI:
10.1111/ppl.12337

45. Two small secreted proteins from Puccinia triticina induce reduction of ß-glucoronidase transient expression in wheat isolines containing Lr9, Lr24 and Lr26

Author:
Vanesa Segovia; Myron Bruce; Jessica L. Shoup Rupp; Li Huang; Guus Bakkeren; Harold N. Trick; John P. Fellers
Source:
Canadian journal of plant pathology 2016 v.38 no.1 pp. 91-102
ISSN:
1715-2992
Subject:
Puccinia recondita; Triticum aestivum; beta-glucuronidase; biolistics; cDNA libraries; cell death; complementary DNA; genes; genomics; genotype; host-pathogen relationships; hypersensitive response; isogenic lines; leaf rust; pathogens; peptides; protein secretion; proteins; wheat
Abstract:
... Little is known about the molecular interaction of wheat and leaf rust (Puccinia triticina Eriks). However, genomic tools are now becoming available so that the host–pathogen interactions can be better understood. Significant efforts are being placed on understanding the secretomes of various pathogens as secreted peptides are believed to be the best candidates for avirulence effectors. In this wo ...
Handle:
10113/62178
DOI:
10.1080/07060661.2016.1150884

46. Vacuolar targeting of r‐proteins in sugarcane leads to higher levels of purifiable commercially equivalent recombinant proteins in cane juice

Author:
Harunipriya Palaniswamy; Divya P. Syamaladevi; Chakravarthi Mohan; Anna Philip; Anushya Petchiyappan; Subramonian Narayanan
Source:
Plant biotechnology journal 2016 v.14 no.2 pp. 791-807
ISSN:
1467-7644
Subject:
Triticum aestivum; beta-glucuronidase; biomass; carbon; crushing; fructans; genes; genetically modified organisms; green fluorescent protein; juices; molecular farming; promoter regions; recombinant proteins; stored products; sucrose; sugarcane; vacuoles
Abstract:
... Sugarcane is an ideal candidate for biofarming applications because of its large biomass, rapid growth rate, efficient carbon fixation pathway and a well‐developed storage tissue system. Vacuoles occupy a large proportion of the storage parenchyma cells in the sugarcane stem, and the stored products can be harvested as juice by crushing the cane. Hence, for the production of any high‐value protein ...
DOI:
10.1111/pbi.12430

47. Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat

Author:
E. Peñaloza; M. Santiago; S. Cabrera; G. Muñoz; L. J. Corcuera; H. Silva
Source:
Biologia plantarum 2017 v.61 no.3 pp. 453-462
ISSN:
0006-3134
Subject:
Arabidopsis thaliana; Triticum aestivum; beta-glucuronidase; container-grown plants; hydroponics; phosphates; progeny; reporter genes; roots; soil; staining; transgenes; transgenic plants; wheat
Abstract:
... The root specificity and phosphate (Pi) deficiency responsiveness of high-affinity phosphate transporter (PHT1) genes point to their promoters as a sustainable system to drive Pi acquisition-related transgenes in plants. In this study, a 3-kb promoter of the AtPHT1;4 gene from Arabidopsis thaliana fused to the β-glucuronidase (GUS) reporter gene was biolistically introduced into wheat (Triticum ae ...
DOI:
10.1007/s10535-016-0672-9

48. Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress

Author:
Pan-Pan Lu; Wei-Jun Zheng; Chang-Tao Wang; Wen-Yan Shi; Jin-Dong Fu; Ming Chen; Jun Chen; Yong-Bin Zhou; Ya-Jun Xi; Zhao-Shi Xu
Source:
BMC plant biology 2018 v.18 no.1 pp. 259
ISSN:
1471-2229
Subject:
Arabidopsis thaliana; Triticum aestivum; beta-glucuronidase; biotic stress; endoplasmic reticulum; gene expression regulation; gene overexpression; genes; heat stress; heat tolerance; heat treatment; mutants; plant growth; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sequence analysis; staining; stress tolerance; wheat
Abstract:
... BACKGROUND: Heat stress is a severe environmental stress that affects plant growth and reduces yield. Bax inhibitor-1 (BI-1) is a cytoprotective protein that is involved in the response to biotic and abiotic stresses. The Arabidopsis (Arabidopsis thaliana) BI-1 mutants atbi1–1 and atbi1–2 are hypersensitive to heat stress, and AtBI-1 overexpression rescues thermotolerance deficiency in atbi1 plant ...
DOI:
10.1186/s12870-018-1485-0
PubMed:
30367612
PubMed Central:
PMC6204060