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1. Direct gene transfer to sugarbeet leaves

Author:
Ivic, S.; Smigocki, A.
Source:
1st Joint IIRB-ASSBT Congress : 27 Feb.-1 Mar, 2003 : San Antonio, Texas, USA = 1er Congrès conjoint IIRB-ASSBT = 1. IIRB-ASSBT Gemeinschaftskongress pp. -
Subject:
Beta vulgaris; sugar beet; genetic transformation; transgenic plants; reporter genes; beta-glucuronidase; leaves; biolistics; gene expression; callus culture; in vitro regeneration
Handle:
10113/12526

2. Die Kaliumhydrogenphosphat-induzierte Nephropathie des Hundes. I. Pathogenese der Tubulusatrophiee [Potassium Hydrogen Phosphate Induced Nephropathy in the Dog. I. Pathogenesis of Tubular Atrophy—author's trans.]

Author:
P. Schneider; G. Pappritz; R. Müller-Peddinghaus; M. Bauer; H. Lehmann; H. Ueberberg; G. Trautwein
Source:
Veterinary pathology v.17 no.6 pp. 699-719
ISSN:
1544-2217
Subject:
Beagle; acid phosphatase; animal pathology; atrophy; basement membrane; beta-glucuronidase; biopsy; blood; body weight; creatinine; detritus; dipotassium hydrogen phosphate; dogs; electron microscopy; epithelial cells; extrusion; fibroblasts; histochemistry; hydrogen; lysosomes; necropsy; nephrocalcinosis; oral administration; pathogenesis; potassium; proteinuria; proximal tubules; sediments; urea nitrogen; vacuoles
Abstract:
... A nephropathy with severe tubular atrophy was observed in Beagle dogs after oral administration of K₂HPO₄ for 14 or 38 weeks. We describe the complete lysosomal degradation of atrophying tubular epithelial cells. During two experiments of 14 and 38 weeks duration, respectively, a total of 15 Beagle dogs received 0.8 g K₂HPO₄/kg body weight daily with their food. All dogs were examined clinically a ...
DOI:
10.1177/030098588001700607

3. Transient expression of foreign genes in rice, wheat and soybean cells following particle bombardment

Author:
Wang, Y.C.; Klein, T.M.; Fromm, M.; Cao, J.; Sanford, J.C.; Wu, R.
Source:
Plant molecular biology 1988 v.11 no.4 pp. 433-439
ISSN:
0167-4412
Subject:
Glycine max; Oryza sativa; Triticum monococcum; genetic transformation; direct DNA uptake; reporter genes; chloramphenicol acetyltransferase; beta-glucuronidase; enzyme activity; plasmids; gene transfer; histochemistry
Abstract:
... The development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. In crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to Agrobacterium-mediated mediated transformation. We have circumvented these difficulties by ...
DOI:
10.1007/BF00039024
PubMed:
24272400

4. Transient gene expression in tobacco protoplasts. II. Comparison of the reporter gene systems for CAT, NPT II, and GUS

Author:
Topfer, R.; Prols, M.; Schell, J.; Steinbiss, H.H.
Source:
Plant cell reports 1988 v.7 no.4 pp. 225-228
ISSN:
0721-7714
Subject:
Nicotiana tabacum; protoplasts; gene expression; chimerism; chloramphenicol acetyltransferase; beta-glucuronidase
DOI:
10.1007/BF00272529
PubMed:
24241753

5. Analysis of a chimeric class-I patatin-GUS gene in transgenic potato plants: high-level expression in tubers and sucrose-inducible expression in cultured leaf and stem explants

Author:
Wenzler, H.C.; Mignery, G.A.; Fisher, L.M.; Park, W.D.
Source:
Plant molecular biology 1989 v.12 no.1 pp. 41-50
ISSN:
0167-4412
Subject:
genetic transformation; genetically modified organisms; stems; beta-glucuronidase; sucrose; Agrobacterium radiobacter; Solanum tuberosum; explants; chimerism; gene expression; tubers; reporter genes; enzyme activity; histochemistry; glycoproteins; leaves; plant proteins
Abstract:
... Patatin is a family of lipid acyl hydrolases that accounts for 30 to 40% of the total soluble protein in potato (Solanum tuberosum L.) tubers. To examine the regulation of the patatin genes, we constructed a chimeric gene containing 2.5 kb of 5' flanking sequence from the class I patatin genomic clone PS20 transcriptionally fused to beta-glucuronidase (GUS) and introduced it into potato plants usi ...
DOI:
10.1007/BF00017446
PubMed:
24272716

6. Cultural and Biochemical Characterization of Actinobacillus and Actinobacillus-Like Species from Ram Lambs with Epididymitis

Author:
Charles M. Scanlan; Mark C. Healey; Anthony R. Torres; Alice V. Johnston
Source:
Journal of veterinary diagnostic investigation v.1 no.4 pp. 288-294
ISSN:
1040-6387
Subject:
Actinobacillus actinomycetemcomitans; Actinobacillus seminis; Bacilli; Haemophilus; Pasteurella; acid phosphatase; alkaline phosphatase; arginine; bacteria; beta-glucuronidase; biotypes; color; epididymitis; glucose; lambs; lysine; nitrates; ornithine; rams
Abstract:
... Cellular, colonial, cultural, and biochemical characteristics of 25 field strains of gram-negative pleomorphic bacilli from rams with epididymitis were compared with Actinobacillus actinomycetemcomitans American Type Culture Collection (ATCC) strain 29522 and Actinobacillus seminis ATCC strain 15768. Three field strains were identified as A. actinomycetemcomitans, 15 as A. seminis, and 2 as Haemop ...
DOI:
10.1177/104063878900100402

7. Bialaphos selection of stable transformants from maize cell culture

Author:
Spencer, T.M.; Gordon-Kamm, W.J.; Daines, R.J.; Start, W.G.; Lemaux, P.G.
Source:
Theoretical and applied genetics 1990 v.79 no.5 pp. 625-631
ISSN:
0040-5752
Subject:
genetic transformation; Zea mays; callus; phosphinothricin acetyltransferase; herbicides; artificial selection; genes; beta-glucuronidase; genetic markers; tissue culture
DOI:
10.1007/BF00226875
PubMed:
24226576

8. Competence for gene transfer by electroporation in a sub-population of protoplasts from uniform carrot cell suspension cultures

Author:
Bower, R.; Birch, R.G.
Source:
Plant cell reports 1990 v.9 no.7 pp. 386-389
ISSN:
0721-7714
Subject:
Daucus carota; cell suspension culture; protoplasts; gene transfer; reporter genes; gene expression; DNA; quantitative analysis; beta-glucuronidase; enzyme activity
DOI:
10.1007/BF00232405
PubMed:
24227061

9. Cotransformation frequencies of foreign genes in soybean cell cultures

Author:
Christou, P.; Swain, W.F.
Source:
Theoretical and applied genetics 1990 v.79 no.3 pp. 337-341
ISSN:
0040-5752
Subject:
genetic transformation; genetically modified organisms; Glycine max; kanamycin kinase; plasmids; gene expression; phosphotransferases (phosphomutases); reporter genes; chloramphenicol acetyltransferase; enzyme activity; beta-glucuronidase; callus; electroporation
DOI:
10.1007/BF01186076
PubMed:
24226351

10. Efficient gene introduction into rice by electroporation and analysis of transgenic plants: use of electroporation buffer lacking chloride ions

Author:
Tada, Y.; Sakamoto, M.; Fujimura, T.
Source:
Theoretical and applied genetics 1990 v.80 no.4 pp. 475-480
ISSN:
0040-5752
Subject:
Oryza sativa; genetic transformation; electroporation; direct DNA uptake; plasmids; organic acids and salts; buffers; genetically modified organisms; gene expression; reporter genes; beta-glucuronidase; phosphotransferases (phosphomutases); Southern blotting
DOI:
10.1007/BF00226748
PubMed:
24221005

11. Gene delivery into cultured plant cells by DNA-coated gold particles accelerated by a pneumatic particle gun

Author:
Iida, A.; Seki, M.; Kamada, M.; Morikawa, H.
Source:
Theoretical and applied genetics 1990 v.80 no.6 pp. 813-816
ISSN:
0040-5752
Subject:
particles; genetic transformation; gene transfer; gold; coatings; direct DNA uptake; laboratory equipment; plasmids; gene expression; Nicotiana tabacum; reporter genes; cell suspension culture; histochemistry; beta-glucuronidase
DOI:
10.1007/BF00224198
PubMed:
24221115

12. Morphological description of transgenic soybean chimeras created by the delivery, integration and expression of foreign DNA using electric discharge particle acceleration

Author:
Christou, P.
Source:
Annals of botany 1990 v.66 no.4 pp. 379-386
ISSN:
0305-7364
Subject:
Glycine max; genetically modified organisms; chimerism; DNA; gene expression; beta-glucuronidase; genetic code; plant morphology; phenotype
Abstract:
... Transgenic soybean (Glycine max L.) plants derived from electric discharge particle acceleration experiments exhibited varying degrees of chimerism which was followed by the expression of the introduced β-glucuronidase (gus) gene. Degrees of chimerism in transgenic plants were established by determining expression of the gus gene observed as blue spots, streaks or sectors in stem and leaf tissues ...
DOI:
10.1093/oxfordjournals.aob.a088039

13. Temporary inhibition of cell wall synthesis improves the transient expression of the GUS gene in Brassica napus mesophyll protoplasts

Author:
Chapel, M.; Glimelius, K.
Source:
Plant cell reports 1990 v.9 no.2 pp. 105-108
ISSN:
0721-7714
Subject:
Brassica napus; mesophyll; protoplasts; genetic transformation; gene expression; cell walls; biosynthesis; beta-glucuronidase; enzyme activity; electroporation
DOI:
10.1007/BF00231560
PubMed:
24226441

14. The effects of promoter on transient expression in conifer cell lines

Author:
Bekkaoui, F.; Datla, R.S.S.; Pilon, M.; Tautorus, T.E.; Crosby, W.L.; Dunstan, D.I.
Source:
Theoretical and applied genetics 1990 v.79 no.3 pp. 353-359
ISSN:
0040-5752
Subject:
Picea glauca; Picea mariana; Pinus banksiana; Cauliflower mosaic virus; genetic transformation; protoplasts; electroporation; plasmids; reporter genes; beta-glucuronidase; chloramphenicol acetyltransferase; gene expression; enzyme activity; somatic embryogenesis; promoter regions; genotype
DOI:
10.1007/BF01186079
PubMed:
24226354

15. Cotransformation and differential expression of introduced genes into potato (Solanum tuberosum L.) cv Bintje

Author:
Ottaviani, M.P.; Cate, C.H.H. ten.
Source:
Theoretical and applied genetics 1991 v.81 no.6 pp. 761-768
ISSN:
0040-5752
Subject:
genetic transformation; genetically modified organisms; kanamycin; Solanum tuberosum; explants; Rhizobium rhizogenes; plasmids; gene expression; clones; phosphotransferases (phosphomutases); rooting; reporter genes; roots; beta-glucuronidase; genetic markers; drug resistance
Abstract:
... The Dutch potato cultivar Bintje has been transformed by Agrobacterium strain LBA1060KG, which contains two plasmids carrying three different DNAs (TL- and TR-DNA on the Agrobacterium rhizogenes plasmid and TKG-DNA on the pBI121 plasmid). Several transformed root clones were obtained after transformation of leaf, stem, and tuber segments, and plants were then regenerated from these root clones. Th ...
DOI:
10.1007/BF00224987
PubMed:
24221438

16. Electroporated protoplasts express seed specific gene promoters

Author:
Fujiwara, T.; Naito, S.; Chino, M.; Nagata, T.
Source:
Plant cell reports 1991 v.9 no.11 pp. 602-606
ISSN:
0721-7714
Subject:
genetic transformation; Catharanthus roseus; protoplast fusion; Glycine max; beta-conglycinin; gene expression; chromosome mapping; Nicotiana tabacum; promoter regions; beta-glucuronidase
Abstract:
... The soybean 7S seed storage protein, beta-conglycinin, is comprised of three major subunits, alpha prime, alpha, and beta. Chimeric genes having beta-conglycinin alpha prime and beta subunit promoters and the beta-glucuronidase gene coding sequence were constructed and electroporated into protoplasts prepared from three cultured cell lines and from tobacco mesophyll cells. The beta-conglycinin pro ...
DOI:
10.1007/BF00231797
PubMed:
24213658

17. Patterns of transformation intensity on flax hypocotyls inoculated with Agrobacterium tumefaciens

Author:
Dong, J.Z.; McHugen, A.
Source:
Plant cell reports 1991 v.10 no.11 pp. 555-560
ISSN:
0721-7714
Subject:
genetic transformation; hypocotyls; Linum usitatissimum; Agrobacterium radiobacter; gene expression; enzyme activity; beta-glucuronidase
Abstract:
... Cellular transformation intensities on flax (Linum usitatissimum) hypocotyl explants using disarmed Agrobacterium tumefaciens were investigated through various preculture durations, cocultivation durations and removal of epidermis. The expression of an intron-containing beta-glucuronidase (GUS) gene driven by CaMV 35S promotor served as a reporter for determination of transformed tissues on hypoco ...
DOI:
10.1007/BF00232510
PubMed:
24221329

18. Polymerase Chain Reaction Used for Monitoring multiple Gene Integration in Agrobacterium-Mediated Transformation

Author:
Nancy K. Blake; Raymond L. Ditterline; Richard G. Stout
Source:
Crop science 1991 v.31 no.6 pp. 1686-1688
ISSN:
0011-183X
Subject:
genetic transformation; gene transfer; genetically modified organisms; Agrobacterium radiobacter; polymerase chain reaction; Medicago sativa; phosphotransferases (phosphomutases); genes; screening; beta-glucuronidase
Abstract:
... Characterizing plants following Agrobacterium-mediated transformation has become an important consideration in the creation of transgenic plants. As an alternative to Southern blot analysis, polymerase chain reaction (PCR) was used to screen for the simultaneous integration of two foreign genes for neomycin phosphotransferase II (NFT II) and β-glucuronidase (GUS) in alfalfa (Medicago saliva L.) tr ...
DOI:
10.2135/cropsci1991.0011183X003100060061x

19. Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts

Author:
Lazzeri, P.A.; Brettschneider, R.; Luhrs, R.; Lorz, H.
Source:
Theoretical and applied genetics 1991 v.81 no.4 pp. 437-444
ISSN:
0040-5752
Subject:
genetic transformation; protoplasts; Hordeum vulgare; direct DNA uptake; polyethylene glycol; phosphotransferases (phosphomutases); reporter genes; cell suspension culture; cell lines; histochemistry; beta-glucuronidase
Abstract:
... Protoplasts isolated from a barley cell suspension (cv Dissa) were transformed with plasmid DNA containing the neomycinphosphotransferase II (NPT) and beta-glucuronidase (GUS) genes, using polyethylene-glycol (PEG) to induce DNA uptake. Transformed microcalli were selected in media containing G418 sulphate. NPT activity was detected in all antibiotic-resistant cell lines, but not all NPT-positive ...
DOI:
10.1007/BF00219433
PubMed:
24221308

20. Transformation of the developing barley endosperm by particle bombardment

Author:
Knudsen, S.; Muller, M.
Source:
Planta 1991 v.185 no.3 pp. 330-336
ISSN:
0032-0935
Subject:
Hordeum vulgare; endosperm; gene expression; hordein; recombinant DNA; reporter genes; beta-glucuronidase; biolistics; genetic transformation; aleurone cells; promoter regions; histochemistry
Abstract:
... Delivery of DNA into intact cells of the developing barley (Hordeum vulgare L.) endosperm was performed with the BIOLISTIC particle gun. It is shown that the proximal 532 base pairs (bp) of the upstream region of a B1-hordein gene drive the expression of the beta-glucuronidase (GUS) gene (uidA) in sub-aleurone and starchy-endosperm cells but not in cells devoid of starch, i.e. developing aleurone ...
DOI:
10.1007/BF00201052
PubMed:
24186414

21. Transient expession of foreign chimeric genes in the gymnosperm hybrid larch following electroporation

Author:
Charest, P.J.; Devantier, Y.; Ward, C.; Jones, C.; Schaffer, U.; Klimaszewska, K.K.
Source:
Canadian journal of botany 1991 v.69 no.8 pp. 1731-1736
ISSN:
1480-3305
Subject:
Larix eurolepis; hybrids; genetic transformation; gene transfer; gene expression; chimerism; beta-glucuronidase; neomycin; phosphotransferases (phosphomutases); chloramphenicol; transferases; enzyme activity
DOI:
10.1139/b91-220

22. Transient expression from microprojectile-mediated DNA transfer in Pinus taeda

Author:
Stomp, A.M.; Weissinger, A.; Sederoff, R.R.
Source:
Plant cell reports 1991 v.10 no.4 pp. 187-190
ISSN:
0721-7714
Subject:
Pinus taeda; genetic transformation; DNA; gene expression; beta-glucuronidase; enzyme activity
Abstract:
... Transfer of plasmid DNA to Pinus taeda L. (loblolly pine) cotyledon cells by microprojectile bombardment has been demonstrated using beta-glucuronidase (GUS). GUS histochemical staining indicated active enzyme in localized centers (blue spots) 24 hours after bombardment. GUS expression declined during subsequent culture, but remained detectable in meristematic tissue 62 days post-bombardment, howe ...
DOI:
10.1007/BF00234292
PubMed:
24221543

23. pEmu: an improved promoter for gene expression in cereal cells

Author:
Last, D.I.; Brettell, R.I.S.; Chamberlain, D.A.; Chaudhury, A.M.; Larkin, P.J.; Marsh, E.L.; Peacock, W.J.; Dennis, E.S.
Source:
Theoretical and applied genetics 1991 v.81 no.5 pp. 581-588
ISSN:
0040-5752
Subject:
Triticum aestivum; Zea mays; Oryza sativa; Triticum monococcum; Lolium multiflorum; Nicotiana plumbaginifolia; genetic transformation; gene expression; protoplasts; direct DNA uptake; electroporation; recombinant DNA; reporter genes; beta-glucuronidase; promoter regions
Abstract:
... A recombinant promoter, pEmu, has been constructed to give a high level of gene expression in monocots. It is based on a truncated maize Adh1 promoter, with multiple copies of the Anaerobic Responsive Element from the maize Adh1 gene and ocs-elements from the octopine synthase gene of Agrobacterium tumefaciens. The pEmu promoter was one of 12 different promoter constructs that were linked to the b ...
DOI:
10.1007/BF00226722
PubMed:
24221371

24. A 126 bp fragment of a plant histone gene promoter confers preferential expression in meristems of transgenic Arabidopsis

Author:
Atanassova, R.; Chaubet, N.; Gigot, C.
Source:
plant journal 1992 v.2 no.3 pp. 291-300
ISSN:
0960-7412
Subject:
Arabidopsis; genetic transformation; Agrobacterium; transgenic plants; histones; gene expression; beta-glucuronidase; enzyme activity; meristems; messenger RNA; chromosome mapping; histochemistry; promoter regions
DOI:
10.1111/j.1365-313X.1992.00291.x
PubMed:
1303796

25. Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv. 'Centennial'

Author:
Ying, M.C.; Dyer, W.E.; Bergman, J.W.
Source:
Plant cell reports 1992 v.11 no.11 pp. 581-585
ISSN:
0721-7714
Subject:
genetic transformation; Carthamus tinctorius; buds; Agrobacterium radiobacter; explants; gene expression; reporter genes; culture media; beta-glucuronidase; callus; shoots
Abstract:
... Efficient callus formation was achieved from cotyledon, stem, and leaf explants of the domestic safflower cultivar 'Centennial' on MS salts medium containing 1 mg/L BAP and 1 mg/L NAA. Shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. 'Centennial' explants inoculated with Agrobacterium tumefacien ...
DOI:
10.1007/BF00233097
PubMed:
24213291

26. Artifacts in the localization of GUS activity in anthers of petunia transformed with a CaMV 35S-GUS construct

Author:
Mascarenhas, J.P.; Hamilton, D.A.
Source:
plant journal 1992 v.2 no.3 pp. 405-408
ISSN:
0960-7412
Subject:
promoter regions; Petunia hybrida; anthers; spatial distribution; transgenic plants; gene expression; Tradescantia paludosa; Cauliflower mosaic virus; enzyme activity; Agrobacterium; beta-glucuronidase; Antirrhinum majus
DOI:
10.1111/j.1365-313X.1992.00405.x

27. Characterization of competent cells and early events of Agrobacterium-mediated genetic transformation in Arabidopsis thaliana

Author:
Sangwan, R.S.; Bourgeois, Y.; Brown, S.; Vasseur, G.; Sangwan-Norreel, B.
Source:
Planta 1992 v.188 no.3 pp. 439-456
ISSN:
0032-0935
Subject:
Arabidopsis thaliana; genetic transformation; cell dedifferentiation; cell walls; transgenic plants; Agrobacterium radiobacter; explants; cotyledons; reporter genes; polysaccharides; cell differentiation; roots; beta-glucuronidase; leaves; tissue culture
Abstract:
... The insertion of foreign DNA in plants occurs through a complex interaction between Agrobacteria and host plant cells. The marker gene beta-glucuronidase of Escherichia coli and cytological methods were used to characterize competent cells for Agrobacterium-mediated transformation, to study early cellular events of transformation, and to identify the potential host-cell barriers that limit transfo ...
DOI:
10.1007/BF00192812
PubMed:
24178335

28. Developmental expression of tomato heat-shock cognate protein 80

Author:
Koning, A.J.; Rose, R.; Comai, L.
Source:
Plant physiology 1992 v.100 no.2 pp. 801-811
ISSN:
0032-0889
Subject:
heat shock proteins; genetic transformation; messenger RNA; plant development; recombinant DNA; structural genes; transgenic plants; amino acid sequences; gene expression; histochemistry; reporter genes; introns; promoter regions; beta-glucuronidase; Solanum lycopersicum var. lycopersicum
Abstract:
... Heat-shock protein 80 (HSP80) is a major heat-shock protein induced in yeast and animals both by heat shock and by specific developmental events. In plants, a heat-shock-induced HSP80 cDNA has been described, although no information concerning developmental regulation of HSP80 genes is available. We have characterized a tomato (Lycopersicon esculentum) gene encoding a typical HSP80 protein. This g ...
DOI:
10.1104/pp.100.2.801
PubMed:
16653061

29. Effect of promoter sequence on transient expression of the beta-glucuronidase gene in embryogenic calli of Larix X eurolepis and Picea mariana following microprojection

Author:
Duchesne, L.C.; Charest, P.J.
Source:
Canadian journal of botany 1992 v.70 no.1 pp. 175-180
ISSN:
0008-4026
Subject:
Larix eurolepis; Picea mariana; callus; gene expression; beta-glucuronidase; enzyme activity; reporter genes; genetic transformation; gene transfer; embryogenesis
DOI:
10.1139/b92-023

30. Expression of the GUS-gene in the monocot tulip after introduction by particle bombardment and Agrobacterium

Author:
Wilmink, A.; Ven, B.C.E. van de.; Dons, J.J.M.
Source:
Plant cell reports 1992 v.11 no.2 pp. 76-80
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; cultivars; Agrobacterium radiobacter; Tulipa; strain differences; Rhizobium rhizogenes; gene expression; beta-glucuronidase
Abstract:
... Gene transfer to the monocotyledon tulip (Tulipa sp. L.) was obtained both by particle bombardment and Agrobacterium transformation. Using a Particle Delivery System, transient expression of the reporter gene for beta-glucuronidase was demonstrated. It was shown that the CAMV 35S as well as the TR2' promoter were active in flower stem explants. Various wildtype and disarmed Agrobacterium strains, ...
DOI:
10.1007/BF00235257
PubMed:
24213489

31. Hormonal and environmental regulation of the carrot lea-class gene Dc3

Author:
Vivekananda, J.; Drew, M.C.; Thomas, T.L.
Source:
Plant physiology 1992 v.100 no.2 pp. 576-581
ISSN:
0032-0889
Subject:
Daucus carota; Nicotiana tabacum; multigene family; recombinant DNA; reporter genes; beta-glucuronidase; gene expression; abscisic acid; water stress; messenger RNA; genetic transformation; transgenic plants; leaf water potential; promoter regions; histochemistry
Abstract:
... Dc3 is a carrot lea-class gene belonging to a small gene family that encodes Dc3 and Dc3-like RNA sequences. We have examined the responsiveness to water deficit and abscisic acid (ABA) of the promoter/enhancer complex of Dc3 fused to a beta-glucuronidase (GUS) reporter gene in vegetative cells of transgenic tobacco. In 56-d tobacco, GUS expression in leaves increased about 200-fold during a 3-d d ...
DOI:
10.1104/pp.100.2.576
PubMed:
16653031

32. Hypotensive effect and enzyme inhibition activity of Mapuche medicinal plant extracts

Author:
Schmeda-Hirschmann, G.; Loyola, J.I.; Sierra, J.; Retamal, R.; Rodriguez, J.
Source:
Phytotherapy research 1992 v.6 no.4 pp. 184-188
ISSN:
0951-418X
Subject:
medicinal plants; plant extracts; antihypertensive agents; enzyme inhibitors; xanthine oxidase; beta-glucuronidase; enzyme activity; American Indians; traditional medicine; Chile
DOI:
10.1002/ptr.2650060404

33. Introduction and differential use of various promoters in pollen grains of Nicotiana glutinosa and Lilium longiflorum

Author:
Leede-Plegt, L.M. van der.; Ven, B.C.E. van de.; Bino, R.J.; Salm, T.P.M. van der.; Tunen, A.J. van.
Source:
Plant cell reports 1992 v.11 no.1 pp. 20-24
ISSN:
0721-7714
Subject:
Nicotiana; Lilium longiflorum; genetic transformation; pollen; gene transfer; chimerism; reporter genes; beta-glucuronidase; gene expression; enzyme activity
Abstract:
... As part of our research to develop an alternative system for the transformation of recalcitrant plant species we investigated the use of the male gametophyte as a transformation vector. Therefore the activity of four different promoters (CaMV 35S, LAT52, chiA PA2 and TR2') was analyzed in pollen of a dicot (Nicotiana glutinosa) and a monocot (Lilium longiflorum) plant species. Gene constructs in w ...
DOI:
10.1007/BF00231833
PubMed:
24213031

34. Prediction of germ-line transformation events in chimeric R0 transgenic soybean plantlets using tissue-specific expression patterns

Author:
Christou, P.; McCabe, D.E.
Source:
plant journal 1992 v.2 no.3 pp. 283-290
ISSN:
0960-7412
Subject:
Glycine max; genetic transformation; germ cells; chimerism; transgenic plants; gene expression; genetic markers; beta-glucuronidase; enzyme activity; genetic improvement; developmental stages; plant anatomy; plant morphology; phenotype
DOI:
10.1111/j.1365-313X.1992.00283.x

35. Regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of Plum Pox Virus

Author:
Camara Machado, M.L. da.; Camara Maahado, A. da.; Hanzer, V.; Weiss, H.; Regner, F.; Steinkellner, H.; Mattanovich, D.; Plail, R.; Knapp, E.; Kalthoff, B.; Katinger, H.
Source:
Plant cell reports 1992 v.11 no.1 pp. 25-29
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; genetically modified organisms; Plum pox virus; Agrobacterium radiobacter; embryo (plant); disease resistance; beta-glucuronidase; genetic markers; Prunus armeniaca
Abstract:
... A system was developed which allows the transfer of foreign genes into apricot cultivars. We report the transformation and regeneration of Prunus armeniaca plants with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids, pBinGUSint, carrying the marker gene beta-glucuronidase (GUS) and pBinPPVm, carrying the coat protein gene of Plum Pox Virus (PPV). The marker gene GUS wa ...
DOI:
10.1007/BF00231834
PubMed:
24213032

36. Role of the leader sequence during thermal repression of translation in maize, tobacco, and carrot protoplasts

Author:
Pitto, L.; Gallie, D.R.; Walbot, V.
Source:
Plant physiology 1992 v.100 no.4 pp. 1827-1833
ISSN:
0032-0889
Subject:
heat shock proteins; protoplasts; Tobacco mosaic virus; Zea mays; messenger RNA; recombinant DNA; Daucus carota; heat stress; Nicotiana tabacum; reporter genes; nucleotide sequences; beta-glucuronidase
Abstract:
... The 5'-untranslated leader of maize (Zea mays) heat-shock protein (hsp) 70 mRNA is required for translational competence during heat shock in protoplasts. When the beta-glucuronidase gene was used as a reporter mRNA, expression at elevated temperatures increased more than 10-fold when the hsp70 leader constituted the 5'-untranslated region. The hsp70 leader did not affect the physical half-life of ...
DOI:
10.1104/pp.100.4.1827
PubMed:
16653204

37. Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants

Author:
Gatz, C.; Frohberg, C.; Wendenburg, R.
Source:
plant journal 1992 v.2 no.3 pp. 397-404
ISSN:
0960-7412
Subject:
Nicotiana tabacum; genetic transformation; Cauliflower mosaic virus; gene transfer; gene expression; tetracycline; transgenic plants; enzyme activity; beta-glucuronidase; messenger RNA; promoter regions
DOI:
10.1111/j.1365-313X.1992.00397.x
PubMed:
1303802

38. Subcellular localization of luteolin glucuronides and related enzymes in rye mesophyll

Author:
Anhalt, S.; Weissenbock, G.
Source:
Planta 1992 v.187 no.1 pp. 83-88
ISSN:
0032-0935
Subject:
protoplasts; glucuronic acid; chemical composition; biosynthesis; spatial distribution; Secale cereale; biochemical pathways; mesophyll; beta-glucuronidase
Abstract:
... Vacuoles were isolated by osmotic rupture of mesophyll protoplasts from the primary leaves of 4-d-and 7-d-old plants of rye (Secate cereale L.). Their content of two flavones, luteolin 7-O-[beta-D-glucuronosyl-(1 leads to 2) beta-D-glucuronide] (R2) and luteolin 7-O-[beta-D-glucuronosyl (1 leads to 2) beta-D-glucuronide]-4'-O-beta-D-glucuronide (R1), as well as that of three specific flavone-glucu ...
DOI:
10.1007/BF00201627
PubMed:
24177970

39. Transformation of vitis tissue by different strains of Agrobacterium tumefaciens containing the T-6b gene

Author:
Berres, R.; Otten, L.; Tinland, B.; Malgarinin-Clog, E.; Walter, B.
Source:
Plant cell reports 1992 v.11 no.4 pp. 192-195
ISSN:
0721-7714
Subject:
genetic transformation; cultivars; Agrobacterium radiobacter; Vitis; genetic engineering; strains; beta-glucuronidase; genetic markers; tissue culture
Abstract:
... Stem pieces and leaf disks of Vitis spp. were cocultured with Agrobacterium tumefaciens strains carrying the UidA (beta-glucuronidase = GUS) gene. The transformation efficiency was highly increased by using a modified T-6b gene (a gene from pTiTm4) which interferes with normal growth and allows regeneration of normal Nicotiana rustica plants (Tinland 1990). The strains first tested on stem segment ...
DOI:
10.1007/BF00232531
PubMed:
24202984

40. Transient gene expression in strawberry (Fragaria X ananassa Duch.) protoplasts and the recovery of transgenic plants

Author:
Nyman, M.; Wallin, A.
Source:
Plant cell reports 1992 v.11 no.2 pp. 105-108
ISSN:
0721-7714
Subject:
Fragaria ananassa; protoplasts; gene transfer; genetically modified organisms; gene expression; beta-glucuronidase; enzyme activity; callus; genetic transformation; electroporation
Abstract:
... A transient beta-glucuronidase (GUS)-assay was performed to evaluate electroporation parameters and optimize DNA delivery conditions into strawberry protoplasts. Optimal GUS-activity was obtained when protoplasts were subjected to 400 V/cm for 20 ms. GUS-activity could be further increased by the addition of carrier DNA to the electroporation mixture. Callus selected on 10 microgram/ml hygromycin ...
DOI:
10.1007/BF00235264
PubMed:
24213496

41. The expression pattern of the tonoplast intrinsic protein gamma-TIP in Arabidopsis thaliana is correlated with cell enlargement

Author:
Ludevid, D.; Hofte, H.; Himelblau, E.; Chrispeels, M.J.
Source:
Plant physiology 1992 v.100 no.4 pp. 1633-1639
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; gene expression; multigene family; structural genes; plant proteins; tonoplast; messenger RNA; recombinant DNA; beta-glucuronidase; reporter genes; genetic transformation; cell growth; stems; petioles; flowers; vascular bundles; histochemistry
Abstract:
... The vacuolar membrane (tonoplast) contains an abundant intrinsic protein with six membrane-spanning domains that is encoded by a small gene family. Different isoforms of tonoplast intrinsic protein (TIP) are expressed in different tissues or as a result of specific signals. Using promoter-beta-glucuronidase (GUS) fusions and in situ hybridization, we have examined the expression of gamma-TIP in Ar ...
DOI:
10.1104/pp.100.4.1633
PubMed:
16653178

42. A 330 bp region of the spinach nitrite reductase gene promoter directs nitrate-inducible tissue-specific expression in transgenic tobacco

Author:
Rastogi, R.; Back, E.; Schneiderbauer, A.; Bowsher, C.G.; Moffatt, B.; Rothstein, S.J.
Source:
plant journal 1993 v.4 no.2 pp. 317-326
ISSN:
0960-7412
Subject:
Nicotiana; genetically modified organisms; beta-glucuronidase; enzyme activity; gene expression; histochemistry; Spinacia oleracea; nitrate reductase; promoter regions
DOI:
10.1046/j.1365-313X.1993.04020317.x

43. Acetosyringone and osmoprotectants like betaine or proline synergistically enhance Agrobacterium-mediated transformation of apple

Author:
James, D.J.; Uratsu, S.; Cheng, J.; Negri, P.; Viss, P.; Dandekar, A.M.
Source:
Plant cell reports 1993 v.12 no.10 pp. 559-563
ISSN:
0721-7714
Subject:
genetic transformation; betaine; Agrobacterium radiobacter; proline; gene expression; Malus domestica; synergism; enzyme activity; syringic acid; culture media; beta-glucuronidase
Abstract:
... The effects of the plant signal molecule acetosyringone (AS) and the osmoprotectant betaine phosphate (BP) have been examined for their ability to increase the transformation efficiency of Agrobacterium tumefaciens (At), C58C1::pGV3850 harboring the binary vector pKIWI105. This binary plasmid encodes the beta-glucuronidase (GUS) gene and was previously shown to be expressed exclusively in plant ti ...
DOI:
10.1007/BF00233060
PubMed:
24201785

44. Activity of the promoter of the Lhca3.St.1 gene, encoding the potato apoprotein 2 of the light-harvesting complex of Photosystem I, in transgenic potato and tobacco plants

Author:
Nap, J.P.; Spanje, M. van.; Dirkse, W.G.; Baarda, G.; Mlynarova, L.; Loonen, A.; Grondhuis, P.; Stiekema, W.J.
Source:
Plant molecular biology 1993 v.23 no.3 pp. 605-612
ISSN:
0167-4412
Subject:
genetic transformation; apoproteins; recombinant DNA; structural genes; transgenic plants; light harvesting complex; Solanum tuberosum; amino acid sequences; gene expression; histochemistry; Nicotiana tabacum; reporter genes; photosystem I; promoter regions; beta-glucuronidase
Abstract:
... We have isolated cDNA and genomic clones for the potato (Solanum tuberosum) apoprotein 2 of the light harvesting complex of Photosystem I, designated Lhca3.St.1. The protein shows all characteristics of the family of chlorophyll a/b-binding proteins. Potato Lhca3.1 gene expression occurs predominantly in leaves, and is transcriptionally regulated by light. One gene copy is present per haploid geno ...
DOI:
10.1007/BF00019307
PubMed:
8219093

45. Agrobacterium-mediated transformation of Asparagus officinalis L. long-term embryogenic callus and regeneration of transgenic plants

Author:
Delbreil, B.; Guerche, P.; Jullien, M.
Source:
Plant cell reports 1993 v.12 no.3 pp. 129-132
ISSN:
0721-7714
Subject:
genetic transformation; genetically modified organisms; Asparagus officinalis; kanamycin; Agrobacterium radiobacter; embryo (plant); embryogenesis; gene expression; genotype; enzyme activity; beta-glucuronidase; callus
Abstract:
... Twenty-three independent kanamycin resistant lines were obtained after cocultivation of long-term embryogenic cultures of three Asparagus officinalis L. genotypes with an Agrobacterium tumefaciens strain harboring beta-glucuronidase and neomycin phosphotransferase II genes. All the lines showed beta-glucuronidase activity by histological staining. DNA analysis by Southern blots of the kanamycin re ...
DOI:
10.1007/BF00239092
PubMed:
24196848

46. Analysis of maize alpha-tubulin gene promoter by transient expression and in transgenic tobacco plants

Author:
Rigau, J.; Capellades, M.; Montoliu, L.; Torres, M.A.; Romera, C.; Martinez-Izquierdo, J.A.; Tagu, D.; Puigdomenech, P.
Source:
plant journal 1993 v.4 no.6 pp. 1043-1050
ISSN:
0960-7412
Subject:
Zea mays; Nicotiana tabacum; genetically modified organisms; beta-glucuronidase; gene expression; genetic techniques and protocols; genetic transformation; histochemistry; protoplasts; tubulin; promoter regions
DOI:
10.1046/j.1365-313X.1993.04061043.x

47. Antisense regulation of the rice waxy gene expression using a PCR-amplified fragment of the rice genome reduces the amylose content in grain starch

Author:
Shimada, H.; Tada, Y.; Kawasaki, T.; Fujimura, T.
Source:
Theoretical and applied genetics 1993 v.86 no.6 pp. 665-672
ISSN:
0040-5752
Subject:
gene transfer; genetic transformation; carbohydrate metabolism; recombinant DNA; seeds; structural genes; amylose; antisense DNA; Oryza sativa; transgenic plants; gene expression; reporter genes; enzyme activity; histochemistry; beta-glucuronidase; callus; starch synthase
Abstract:
... The waxy gene encodes a granule-bound starch synthase. A 1.0-kb portion of the sequence of the rice waxy gene, which includes the region between exon 4 and exon 9, was inserted in an antisense orientation between the 35 S promoter and the GUS gene of pBI221. The resultant plasmid, pWXA23, was introduced into rice protoplasts by electroporation. GUS activity was clearly detected in derived callus l ...
DOI:
10.1007/BF00222654
PubMed:
24193774

48. Characterization of the catalase antioxidant defense gene Cat1 of maize, and its developmentally regulated expression in transgenic tobacco

Author:
Lingqiang Guan; John G. Scandalios
Source:
plant journal 1993 v.3 no.4 pp. 527-536
ISSN:
0960-7412
Subject:
flowering; germination; catalase; transgenic plants; DNA; corn; pollen; tissues; Nicotiana tabacum; gene fusion; antioxidant activity; introns; beta-glucuronidase; tobacco
Abstract:
... A genomic clone containing the maize Cat1 gene has been isolated and its complete DNA sequence determined. The start of transcription has been mapped by primer extension. Six introns were identified in the Cat1 coding region. In order to determine the tissue‐specific expression pattern of the Cat1 gene, promoter‐reporter gene fusion constructs were made consisting of 2.5 kb and 0.8 kb of the 5′Cat ...
DOI:
10.1046/j.1365-313X.1993.03040527.x

49. Combinational interactions between positive and negative cis-acting elements control spatial patterns of 4CL-1 expression in transgenic tobacco

Author:
Hauffe, K.D.; Lee, S.P.; Subramaniam, R.; Douglas, C.J.
Source:
plant journal 1993 v.4 no.2 pp. 235-253
ISSN:
0960-7412
Subject:
Nicotiana; genetically modified organisms; beta-glucuronidase; enzyme activity; gene expression; genetic transformation; metabolism; promoter regions
DOI:
10.1046/j.1365-313X.1993.04020235.x

50. Common bean root exudates contain elevated levels of daidzein and coumestrol in response to Rhizobium inoculation

Author:
Dakora, F.D.; Joseph, C.M.; Phillips, D.A.
Source:
Molecular plant-microbe interactions 1993 v.6 no.5 pp. 665-668
ISSN:
0894-0282
Subject:
Phaseolus vulgaris; Rhizobium leguminosarum; Rhizobium phaseoli; daidzein; coumestrol; root exudates; gene expression; structural genes; recombinant DNA; beta-glucuronidase; promoter regions; transcription (genetics); histochemistry
DOI:
10.1094/MPMI-6-665

51. Conserved regulation of the soybean early nodulin ENOD2 gene promoter in determinate and indeterminate transgenic root nodules

Author:
Lauridsen, Peter; Franssen, Henk; Stougaard, Jens; Bisseling, Ton; Marcker, Kjeld A.
Source:
plant journal 1993 v.3 no.3 pp. 483-492
ISSN:
0960-7412
Subject:
DNA; Lotus corniculatus; Trifolium repens; Trigonella corniculata; beta-glucuronidase; genes; nucleotide sequences; root nodules; soybeans; transcription factors
Abstract:
... The β-glucuronidase (GUS) activity expressed from the soybean early nodulin ENOD2(B) gene promoter was localized histochemically in nodules of Lotus corniculatus and Trifolium repens. In both the determinate Lotus nodules and the indeterminate Trifolium nodules, activity was found in the parenchyma cells and especially in cells close to the vascular tissue of nodules. The characteristic cell-speci ...
DOI:
10.1111/j.1365-313X.1993.tb00168.x

52. Differential gene expression in nematode-induced feeding structures of transgenic plants harbouring promoter-gusA fusion constructs

Author:
Goddijn, O.J.M.; Lindsey, K.; Lee, F.M. van der.; Klap, J.C.; Sijmons, P.C.
Source:
plant journal 1993 v.4 no.5 pp. 863-873
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; Nicotiana tabacum; beta-glucuronidase; genetic transformation; genetically modified organisms; gene expression; Heterodera schachtii; Meloidogyne incognita; promoter regions
DOI:
10.1046/j.1365-313X.1993.04050863.x
PubMed:
8275103

53. Efficient and senitive assay for T-DNA-dependent transient gene expression

Author:
Rossi, L.; Escudero, J.; Hohn, B.; Tinland, B.
Source:
Plant molecular biology reporter 1993 v.11 no.3 pp. 220-229
ISSN:
0735-9640
Subject:
quantitative analysis; seedlings; transgenic plants; Agrobacterium radiobacter; gene expression; Nicotiana tabacum; enzyme activity; beta-glucuronidase
DOI:
10.1007/BF02669849

54. Electroporation of rapeseed protoplasts – transient and stable transformation

Author:
Per Bergman; Kristina Glimelius
Source:
Physiologia plantarum 1993 v.88 no.4 pp. 604-611
ISSN:
0031-9317
Subject:
Brassica napus; X-radiation; antibiotic resistance; beta-glucuronidase; callus; electroporation; gene transfer; genes; genetic markers; hypocotyls; irradiation; kanamycin kinase; neomycin; plasmids; protoplasts; rapeseed; transgenic plants
Abstract:
... Protoplasts of Brassica napus hypocotyls were transfected using electroporation. Parameters such as discharge potential, protoplast density and buffer constituents were tested to determine the most suitable conditions for gene transfer. To monitor the introduction of DNA into protoplasts a plasmid containing the β‐glucuronidase (EC 3.2.1.31), and the neomycin phospotransferase (EC 2.7.1.95) genes ...
DOI:
10.1111/j.1399-3054.1993.tb01378.x

55. Expression in cowpea seedlings of chimeric transgenes after electroporation into seed derived embryos

Author:
Akella, V.; Lurquin, P.F.
Source:
Plant cell reports 1993 v.12 no.2 pp. 110-117
ISSN:
0721-7714
Subject:
Vigna unguiculata; seedlings; genetic transformation; genetically modified organisms; gene expression; chimerism; electroporation; embryo (plant); beta-glucuronidase; enzyme activity
Abstract:
... Cowpea (Vigna unguiculata Walp) embryos mechanically isolated from mature seeds and incubated in the presence of plasmid DNA harboring chimeric gus genes were shown to germinate into seedlings expressing beta-glucuronidase activity in a variety of tissues, including the apical meristem. Embryo electroporation in the presence of DNA and protectants such as spermine and LipofectinTM increased both t ...
DOI:
10.1007/BF00241945
PubMed:
24202079

56. Expression of an Arabidopsis sucrose synthase gene indicates a role in metabolization of sucrose both during phloem loading and in sink organs

Author:
Martin, T.; Frommer, W.B.; Salanoubat, M.; Willmitzer, L.
Source:
plant journal 1993 v.4 no.2 pp. 367-377
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; Nicotiana; genetically modified organisms; amino acid sequences; gene expression; genetic transformation; metabolism; phloem loading; sucrose synthase; beta-glucuronidase; histochemistry; transcription (genetics)
DOI:
10.1046/j.1365-313X.1993.04020367.x
PubMed:
8220487

57. Expression of antisense nodulin‐35 RNA in Vigna aconitifolia transgenic root nodules retards peroxisome development and affects nitrogen availability to the plant

Author:
Na‐Gyong Lee; Barry Stein; Hideki Suzuki; Desh Pal S. Verma
Source:
plant journal 1993 v.3 no.4 pp. 599-606
ISSN:
0960-7412
Subject:
Bradyrhizobium; RNA; Rhizobium rhizogenes; Vigna aconitifolia; antibodies; beta-glucuronidase; biosynthesis; cDNA libraries; complementary DNA; cowpeas; genes; genetically modified organisms; nitrogen; peroxisomes; polymerase chain reaction; root nodules; sequence analysis; soybeans; ureides
Abstract:
... A nodulin‐35 (N‐35) cDNA encoding nodule‐specific uricase (EC 1.7.3.3.) was isolated from a Vigna aconitifolia (mothbean) root nodule cDNA library. Sequence analysis of Vigna uricase (VN‐35) cDNA revealed 90% homology to that of soybean. The VN‐35 cDNA was inserted in the antisense orientation downstream of the CaMV—35S promoter, and transgenic hairy roots were formed on Vigna plants using Agrobac ...
DOI:
10.1046/j.1365-313X.1993.03040599.x

58. Factors affecting transient gene expression in protoplasts isolated from very slowly growing embryogenic callus cultures of wheat (Triticum aestivum L.)

Author:
Zaghmout, O.M.F.; Trolinder, N.L.
Source:
Theoretical and applied genetics 1993 v.86 no.6 pp. 721-730
ISSN:
0040-5752
Subject:
alcohol dehydrogenase; protoplasts; genetic transformation; Tobacco mosaic virus; recombinant DNA; transgenic plants; enzyme activity; plasmid vectors; gene expression; histochemistry; Triticum aestivum; reporter genes; Cauliflower mosaic virus; Vitis vinifera; embryogenesis; promoter regions; beta-glucuronidase; callus; electroporation
Abstract:
... Protoplasts isolated from embryogenic ('Mustang' and 'Chinese Spring') and non-embryogenic ('Mit') calli of wheat (Triticum aestivum L.) genotypes transiently expressed beta-glucuronidase (GUS) activity when electroporated with a plasmid containing the GUS gene and driven by an enhanced 35S promoter and a TMV leader sequence. Conditions for the maximum expression of GUS activity were: electroporat ...
DOI:
10.1007/BF00222662
PubMed:
24193782

59. Factors influencing tDNA transfer in Agrobacterium-mediated transformation of sugarbeet

Author:
Jacq, B.; Lesobre, O.; Sangwan, R.S.; Sangwan-Norreel, B.S.
Source:
Plant cell reports 1993 v.12 no.11 pp. 621-624
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; hypocotyls; DNA; Agrobacterium radiobacter; explants; cotyledons; syringic acid; genotype; genetic variation; introns; culture media; beta-glucuronidase; Beta vulgaris
Abstract:
... Agrobacterium-mediated transformation of sugarbeet (Beta vulgaris L.) was investigated for T-DNA transfer efficiency, using an intron containing beta-glucuronidase gene. Preculture and coculture of hypocotyl and cotyledon explants with acetosyringone upon infection was studied. Seven seed lots which included several hundred genotypes, were screened, and were all susceptible to T-DNA transfer but w ...
DOI:
10.1007/BF00232811
PubMed:
24201875

60. Factors influencing transient gene expression in bean (Phaseolus vulgaris L.) using an electrical particle acceleration device

Author:
Aragao, F.J.L.; Grossi de Sa, M.F.; Davey, M.R.; Brasileiro, A.C.M.; Faria, J.C.; Rech, E.L.
Source:
Plant cell reports 1993 v.12 no.9 pp. 483-490
ISSN:
0721-7714
Subject:
apical meristems; genetic transformation; transgenic plants; direct DNA uptake; laboratory equipment; cotyledons; Phaseolus vulgaris; gene expression; reporter genes; beta-glucuronidase; leaves
Abstract:
... The parameters influencing transient expression of the beta-glucuronidase gene in bean embryonic axes, cotyledons, apical meristems and leaves were evaluated after gene delivery with an electrical particle acceleration device. A calcium-spermidine procedure for coating gold particles with DNA resulted in higher levels of GUS expression with lower concentrations of gold particles compared with a ca ...
DOI:
10.1007/BF00236092
PubMed:
24196106

61. Field performance of derived generations of transgenic tobacco

Author:
Caligari, P.D.S.; Yapabandara, Y.M.H.B.; Paul, E.M.; Perret, J.; Roger, P.; Dunwell, J.M.
Source:
Theoretical and applied genetics 1993 v.86 no.7 pp. 875-879
ISSN:
0040-5752
Subject:
genetic transformation; kanamycin; transgenic plants; Agrobacterium radiobacter; agronomic traits; crop yield; phosphotransferases (phosphomutases); Nicotiana tabacum; reporter genes; field experimentation; beta-glucuronidase; drug resistance
Abstract:
... Two inbred cultivars of Nicotiana tabacum (tobacco), 'Samsun' and 'Xanthi', were transformed with the plasmid pBI 121 using Bin 19 in Agrobacterium tumefaciens. The plasmid carries the nptII gene conferring kanamycin resistance and the uidA gene encoding beta-glucuronidase (GUS). Progeny carrying the genes in the homozygous condition were identified and selfed over several generations. One line ho ...
DOI:
10.1007/BF00212615
PubMed:
24193884

62. Gene transfer to maize male reproductive structure by particle bombardment of tassel primordia

Author:
Dupuis, I.; Pace, G.M.
Source:
Plant cell reports 1993 v.12 no.11 pp. 607-611
ISSN:
0721-7714
Subject:
Zea mays; pollen; gene transfer; DNA; anther culture; gene expression; beta-glucuronidase; anthocyanins; genetic markers
Abstract:
... Maize (Zea mays L.) tassel primordia were used as a target for particle bombardment, to assess the possibility of introducing foreign DNA into male reproductive structures. Transient expression of the beta-glucuronidase gene (GUS) or anthocyanin marker genes (C1 and B-Peru) driven by the CaMV 35S promoter was obtained in tassel primordia 24h after bombardment. Gold particles coated with DNA reache ...
DOI:
10.1007/BF00232808
PubMed:
24201872

63. Genetic transformation in the grain legume Cicer arietinum L. (chickpea)

Author:
Fontana, G.S.; Santini, L.; Caretto, S.; Frugis, G.; Mariotti, D.
Source:
Plant cell reports 1993 v.12 no.4 pp. 194-198
ISSN:
0721-7714
Subject:
genetic transformation; genetically modified organisms; Agrobacterium radiobacter; embryo (plant); enzyme activity; gene expression; phosphotransferases (phosphomutases); genomics; neomycin; Cicer arietinum; beta-glucuronidase; shoots
Abstract:
... In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for Agrobacterium-mediated gene transfer by the co-cultivation technique. Chickpea transgenic plants showed neomycin phosphotransferase II and beta-glucu ...
DOI:
10.1007/BF00237052
PubMed:
24197018

64. Interaction between the tobacco DNA-binding activity CBF and the cyt-1 promoter element of the Agrobacterium tumefaciens T-DNA gene T-CYT correlates with cyt-1 directed gene expression in multiple tobacco tissue types

Author:
Neuteboom, S.T.C.; Stoffels, E.; Hulleman, E.; Memelink, J.; Schilperoort, R.A.; Hoge, J.H.C.
Source:
plant journal 1993 v.4 no.3 pp. 525-534
ISSN:
0960-7412
Subject:
gene transfer; Nicotiana; Agrobacterium radiobacter; transcription (genetics); gene expression; DNA-binding proteins; mutants; enzyme activity; promoter regions; beta-glucuronidase
DOI:
10.1046/j.1365-313X.1993.04030525.x
PubMed:
8220494

65. Milk fat globule membrane substances inhibit mouse intestinal beta-glucuronidase

Author:
Ito, O.; Hayashi, M.; Ushiyama, K.
Source:
Journal of food science 1993 v.58 no.4 pp. 753-796
ISSN:
0022-1147
Subject:
milk fat; beta-glucuronidase; intestines; mice
Abstract:
... The beta-glucuronidase inhibitory action of milk fat globule membrane (MFGM) was studied. By increasing the MFGM concentration to 0.2%, nearly 90% of the beta-glucuronidase activity was inhibited. The trypsin-derived digests of MFGM inhibited beta-glucuronidase activity by approximately 42%. The inhibitory effect of MFGM on intestinal beta-glucuronidase of mouse was studied. We administered MFGM ( ...
DOI:
10.1111/j.1365-2621.1993.tb09351.x

66. Molecular characterization of one of the maize polygalacturonase gene family members which are expressed during late pollen development

Author:
Allen, Rebecca L.; Lonsdale, David M.
Source:
plant journal 1993 v.3 no.2 pp. 261-271
ISSN:
0960-7412
Subject:
Escherichia coli; Oenothera; RNA; beta-glucuronidase; complementary DNA; corn; gene expression; genes; in situ hybridization; leaves; mitosis; pollen; polygalacturonase; tobacco; tomatoes
Abstract:
... A gene exhibiting homology to the polygalacturonases of several species, including tomato and Oenothera, has been shown by RNA dot-blot analysis and in situ hybridization experiments to be expressed post-first microspore mitosis in maize. A 2.87 kbp section of the promoter fused to E. coli β-glucuronidase (uidA) coding sequence conferred the correct spatial and temporal expression in transgenic to ...
DOI:
10.1111/j.1365-313X.1993.tb00177.x

67. Molecular characterization of the gene for carrot cell wall beta-fructosidase

Author:
Ramioch-Lorenz, K.; Knudsen, S.; Sturm, A.
Source:
plant journal 1993 v.4 no.3 pp. 545-554
ISSN:
0960-7412
Subject:
genetically modified organisms; messenger RNA; genetic code; Daucus carota; transcription (genetics); gene expression; cell suspension culture; promoter regions; beta-glucuronidase
DOI:
10.1046/j.1365-313X.1993.04030545.x
PubMed:
8220495

68. Myb genes from Hordeum vulgare: tissue-specific expression of chimeric Myb promoter/Gus genes in transgenic tobacco

Author:
Wissenbach, M.; Uberlacker, B.; Vogt, F.; Becker, D.; Salamini, F.; Rohde, W.
Source:
plant journal 1993 v.4 no.3 pp. 411-422
ISSN:
0960-7412
Subject:
genetically modified organisms; Nicotiana; Hordeum vulgare; transcription (genetics); gene expression; DNA-binding proteins; enzyme activity; promoter regions; beta-glucuronidase
DOI:
10.1046/j.1365-313X.1993.04030411.x
PubMed:
8220488

69. Nonlethal assay system of beta-glucuronidase activity in transgenic tobacco roots

Author:
Kirchner, G.; Kinslow, C.J.; Bloom, G.C.; Taylor, D.W.
Source:
Plant molecular biology reporter 1993 v.11 no.4 pp. 320-325
ISSN:
0735-9640
Subject:
Nicotiana tabacum; transgenic plants; genetic transformation; beta-glucuronidase; enzyme activity; assays; reporter genes; histochemistry
DOI:
10.1007/BF02905333

70. Optimization of biolistic transformation of embryogenic grape cell suspensions

Author:
Hebert, D.; Kikkert, J.R.; Smith, F.D.; Reisch, B.I.
Source:
Plant cell reports 1993 v.12 no.10 pp. 585-589
ISSN:
0721-7714
Subject:
neomycin; hybrids; Vitis riparia; tungsten; gene expression; interspecific hybridization; Vitis vinifera; diameter; Vitis labrusca; Vitis rupestris; phosphotransferases (phosphomutases); helium; enzyme activity; culture media; genetic transformation; gene transfer; activated carbon; pressure; kanamycin; transgenic plants; embryo (plant); charcoal; cell suspension culture; beta-glucuronidase
Abstract:
... Embryogenic suspensions of 'Chancellor' (Vitis L. complex interspecific hybrid) were bombarded with tungsten particles coated with plasmid pBI426 encoding glucuronidase (GUS) and neomycin phosphotransferase (NPTII) which results in kanamycin resistance. Two d after bombardment, cultures were placed on semi-solid medium containing either 8.6 or 17.2 micromolar kanamycin. Factors that affect biolist ...
DOI:
10.1007/BF00233066
PubMed:
24201791

71. Pathogen, salicylic acid and developmental dependent expression of a beta-1,3-glucanase/GUS gene fusion in transgenic tobacco plants

Author:
Hennig, J.; Dewey, R.E.; Cutt, J.R.; Klessig, D.F.
Source:
plant journal 1993 v.4 no.3 pp. 481-493
ISSN:
0960-7412
Subject:
Tobacco mosaic virus; genetically modified organisms; genetic code; beta-glucanase; gene expression; transcription (genetics); Nicotiana tabacum; salicylic acid; promoter regions; beta-glucuronidase; leaves
DOI:
10.1046/j.1365-313X.1993.04030481.x
PubMed:
8220491

72. Preparation of a dietary fiber mixture derived from different sources and its metabolic effects if rats

Author:
Hazan, A.; Madar, Z.
Source:
Journal of the American College of Nutrition 1993 v.12 no.6 pp. 661-668
ISSN:
0731-5724
Subject:
metabolism; beta-glucuronidase; rats; dietary fiber
Abstract:
... In this study we analyzed a commercial dietary fiber blend known as Fiber-Plus and a dietary fiber mixture (DFM) prepared in our lab (10% apple pectin, 10% orange pectin, 36% locust bean gum and 44% corncob composed of 56% fiber). DFM provided greater inhibition of starch digestion and glucose absorption (p < 0.05) than did Fiber-Plus. DFM was also found to be significantly more efficient (p < 0.0 ...
DOI:
10.1080/07315724.1993.10718357

73. Production and germination of oospores of Albugo candida

Author:
Liu, J.Q.; Rimmer, S.R.
Source:
Canadian journal of plant pathology 1993 v.15 no.4 pp. 265-271
ISSN:
0706-0661
Subject:
sporulation; laboratory techniques; arylsulfatase; cooling; Brassica juncea; maturity stage; oospores; Albugo candida; plant pathogenic fungi; spore germination; Brassica rapa subsp. oleifera; beta-glucuronidase
DOI:
10.1080/07060669309501922

74. Promoter and leader regions involved in the expression of the Arabidopsis ferredoxin A gene

Author:
Timothy Caspar; Peter H. Quail
Source:
plant journal 1993 v.3 no.1 pp. 161-174
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; beta-glucuronidase; electron transfer; gene expression; genetic databases; luciferase; messenger RNA; photoreceptors; photosynthesis; phytochrome; plastids; reporter genes; roots; seedlings; staining; tissues; transcription (genetics); white light
Abstract:
... Ferredoxin is a nuclear‐encoded protein that is involved in a variety of electron transfer reactions in both photosynthetic and non‐photosynthetic plastids. We show here that the expression of the ferredoxin A gene (FedA) in Arabidopsis thaliana is light‐regulated, with its mRNA level increased 4.5‐fold by transfer of dark‐grown seedlings to white light for 3 h. A portion of this light regulation ...
DOI:
10.1046/j.1365-313X.1993.t01-8-00999.x

75. Regeneration of transgenic tamarillo plants

Author:
Atkinson, R.G.; Gardner, R.C.
Source:
Plant cell reports 1993 v.12 no.6 pp. 347-351
ISSN:
0721-7714
Subject:
organogenesis; genetic transformation; gene transfer; kanamycin; transgenic plants; Agrobacterium radiobacter; explants; Solanum betaceum; gene expression; enzyme activity; culture media; beta-glucuronidase; leaves; shoots
Abstract:
... Media were developed to regenerate shoots from leaf pieces of tamarillo (Cyphomandra betacea (Cav.) Sendtner). Shoots were derived via organogenesis and could be easily rooted and transferred to the growth chamber. Transgenic tamarillo plants were produced using the binary vector pKIWI110 in the avirulent Agrobacterium strain LBA4404. All transgenic plants were kanamycin resistant and some plants ...
DOI:
10.1007/BF00237433
PubMed:
24197262

76. Regulation of nitrate and nitrite reductase expression in Nicotiana plumbaginifolia leaves by nitrogen and carbon metabolites

Author:
Vincentz, Michel; Moureaux, Thérèse; Leydecker, Marie-Thérèse; Vaucheret, Hervé; Caboche, Michel
Source:
plant journal 1993 v.3 no.2 pp. 315-324
ISSN:
0960-7412
Subject:
Nicotiana plumbaginifolia; beta-glucuronidase; carbon; complementary DNA; fructose; gene expression; glucose; glutamine; leaves; light intensity; messenger RNA; metabolites; nitrate reduction; nitrite reductase; nitrogen; reporter genes; structural genes; sucrose; tobacco; transgenic plants
Abstract:
... Nitrate (NR) and nitrite reductase (NiR) catalyse the reduction of nitrate to ammonium. The regulation of NR and NiR gene expression by carbohydrates (C) and nitrogen (N) metabolites was studied using detached leaves. In the dark, glucose, fructose and sucrose supplied to detached green leaves of dark-adapted Nicotiana plumbaginifolia plants resulted in NR mRNA and protein accumulation and the los ...
DOI:
10.1111/j.1365-313X.1993.tb00183.x

77. Response of a nitrite-reductase 3.1-kilobase upstream regulatory sequence from spinach to nitrate and light in transgenic tobacco

Author:
Neininger, A.; Bichler, J.; Schneiderbauer, A.; Mohr, H.
Source:
Planta 1993 v.189 no.3 pp. 440-442
ISSN:
0032-0935
Subject:
Spinacia oleracea; Nicotiana tabacum; oxidoreductases; recombinant DNA; reporter genes; beta-glucuronidase; gene expression; nitrates; light; transgenic plants; genetic transformation; messenger RNA; promoter regions; gene fusion
Abstract:
... In the present study the question was addressed of whether the nitrite-reductase (NIR-)promoter from spinach (Spinacia oleracea L.). fused to a reporter gene (bacterial beta-glucuronidase, GUS) and introduced into tobacco (Nicotiana tabacum L.) responds to nitrate and light in accordance with spinach (donor) or in accordance with tobacco (host). The data obtained at the GUS enzyme level as well as ...
DOI:
10.1007/BF00194443
PubMed:
24178503

78. Spatial-temporal colonization patterns of Azospirillum brasilense on the wheat root surface and expression of the bacterial nifH gene during association

Author:
Broek, A.V.; Michiels, J.; Gool, A. van.; Vanderleyden, J.
Source:
Molecular plant-microbe interactions 1993 v.6 no.5 pp. 592-600
ISSN:
0894-0282
Subject:
temporal variation; promoter regions; nitrogen fixation; recombinant DNA; spatial distribution; Azospirillum brasilense; histochemistry; Triticum aestivum; adhesion; acetylene; reporter genes; nitrogenase; roots; beta-glucuronidase
DOI:
10.1094/MPMI-6-592

79. Tissue-specific and wound-inducible pattern of expression of the mannopine synthase promoter is determined by the interaction between positive and negative cis-regulatory elements

Author:
Guevara-Garcia, A.; Mosqueda-Cano, G.; Arguello-Astorga, G.; Simpson, J.; Herrera-Estrella, L.
Source:
plant journal 1993 v.4 no.3 pp. 495-505
ISSN:
0960-7412
Subject:
genetically modified organisms; Nicotiana; genetic code; Agrobacterium radiobacter; gene expression; promoter regions; beta-glucuronidase
DOI:
10.1046/j.1365-313X.1993.04030495.x
PubMed:
8220492

80. Transcient and stable expression of gusA fusions with rice genes in rice, barley and perennial ryegrass

Author:
Hensgens, L.A.M.; Bakker, E.P.H.M. de.; Os-Ruygrok, E.P. van.; Rueb, S.; Mark, F. van de.; Maas, H.M. van der.; Veen, S. van der.; Kooman-Gersmann, M.; Hart, L.; Schilperoort, R.A.
Source:
Plant molecular biology 1993 v.23 no.4 pp. 643-669
ISSN:
0167-4412
Subject:
Oryza sativa; Lolium perenne; Hordeum vulgare; genetic transformation; transgenic plants; reporter genes; recombinant DNA; beta-glucuronidase; gene expression; callus; introns; plant proteins; promoter regions; histochemistry
Abstract:
... Transcriptional and translational fusions were made between the reading frame coding for beta-D-glucuronidase and sequences of either a constitutively expressed rice gene (GOS2) involved in initiation of translation or a light-inducible rice gene (GOS5). The transient expression of the fusions was studied via particle bombardment of seedling tissues of rice, perennial ryegrass and barley. Furtherm ...
DOI:
10.1007/BF00021522

81. Transcription of two members of a gene family encoding phenylalanine ammonia‐lyase leads to remarkably different cell specificities and induction patterns

Author:
Diane Shufflebottom; Keith Edwards; Wolfgang Schuch; Michael Bevan
Source:
plant journal 1993 v.3 no.6 pp. 835-845
ISSN:
0960-7412
Subject:
Arabidopsis; beta-glucuronidase; biosynthesis; gene expression; gene expression regulation; green beans; messenger RNA; phenylalanine ammonia-lyase; plant development; potatoes; reporter genes; tissues; tobacco; transcription (genetics)
Abstract:
... Phenylalanine ammonia‐lyase (PAL) catalyses the first committed step in the biosynthesis of phenyl‐propanoids, which perform a variety of functions in plant development and in their interactions with the environment. French bean contains a small family of genes encoding PAL and two of these genes, PAL2 and PAL3, have been shown to be differentially expressed at the mRNA level in bean tissues. The ...
DOI:
10.1111/j.1365-313X.1993.00835.x

82. Transformation of maize (Zea mays L.) protoplasts and regeneration of haploid transgenic plants

Author:
Sukhapinda, K.; Kozuch, M.E.; Rubin-Wilson, B.; Ainley, W.M.; Merlo, D.J.
Source:
Plant cell reports 1993 v.13 no.2 pp. 63-68
ISSN:
0721-7714
Subject:
Southern blotting; protoplasts; genetic transformation; Zea mays; genetic code; beta-glucuronidase; transgenic plants; plasmids; phosphotransferases (phosphomutases); genetic markers; artificial regeneration; neomycin; enzyme activity; culture media; cell suspension culture; callus; electroporation
Abstract:
... Transgenic haploid maize (Zea mays L.) plants were obtained from protoplasts isolated from microspore-derived cell suspension cultures. Protoplasts were electroporated in the presence of plasmid DNA containing the gus A and npt II genes encoding beta-glucuronidase (GUS) and neomycin phosphotransferase II (NPT II), respectively. Transformed calli were selected and continuously maintained on kanamyc ...
DOI:
10.1007/BF00235291
PubMed:
24196288

83. Transformation of peas

Author:
Davies, D.R.; Hamilton, J.; Mullineaux, P.
Source:
Plant cell reports 1993 v.12 no.3 pp. 180-183
ISSN:
0721-7714
Subject:
genetic transformation; kanamycin; DNA; Agrobacterium radiobacter; meristems; cotyledons; gene expression; nucleotide sequences; reporter genes; Pisum sativum; beta-glucuronidase; genetic markers
Abstract:
... The lateral cotyledonary meristems present in germinating seed were inoculated with a non-oncogenic strain of A. tumefaciens carrying a gene conferring kanamycin resistance as a selectable marker and a beta-glucuronidase sequence as a reporter gene. Kanamycin resistant plants were derived from the meristems and shown to be transformed on the basis of Southern blots, polymerase chain reaction analy ...
DOI:
10.1007/BF00239102
PubMed:
24196858

84. Transformation of pecan and regeneration of transgenic plants

Author:
McGranahan, G.H.; Leslie, C.A.; Dandekar, A.M.; Uratsu, S.L.; Yates, I.E.
Source:
Plant cell reports 1993 v.12 no.11 pp. 634-638
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; kanamycin; Juglans regia; transgenic plants; Agrobacterium radiobacter; Carya illinoinensis; genotype; genetic variation; culture media; beta-glucuronidase; genetic markers; somatic embryos
Abstract:
... A gene transfer system developed for walnut (Juglans regia L.) was successfully applied to pecan (Carya illinoensis [Wang] K. Koch). Repetitively embryogenic somatic embryos derived from open-pollinated seed of 'Elliott', 'Wichita', and 'Schley' were co-cultivated with Agrobacterium strain EHA 101/pCGN 7001, which contains marker genes for beta-glucuronidase activity and resistance to kanamycin. S ...
DOI:
10.1007/BF00232814
PubMed:
24201878

85. Transformation of white spruce (Picea glauca) somatic embryos by microprojectile bombardment

Author:
Bommineni, V.R.; Chibbar, R.N.; Datla, R.S.S.; Tsang, E.W.T.
Source:
Plant cell reports 1993 v.13 no.1 pp. 17-23
ISSN:
0721-7714
Subject:
Picea glauca; embryo culture; micropropagation; gene transfer; beta-glucuronidase; enzyme activity; gene expression; genetic transformation
Abstract:
... Cotyledonary somatic embryos of white spruce [Picea glauca (Moench) Voss] were subjected to microprojectile bombardment with a gene construct containing a gus::nptII fusion gene. Somatic embryos were used to re-induce the embryogenic tissue after bombardments. Histochemical assay using X-gluc as a substrate showed that all the embryos (100%) were GUS positive 48 h after bombardment. However, only ...
DOI:
10.1007/BF00232308
PubMed:
24196176

86. Transgenic plants of ramie (Boehmeria nivea Gaud.) obtained by Agrobacterium mediated transformation

Author:
Dusi, D.M.A.; Dubald, M.; Almeida, E.R.P. de.; Caldas, L.S.; Gander, E.S.
Source:
Plant cell reports 1993 v.12 no.11 pp. 625-628
ISSN:
0721-7714
Subject:
genetic transformation; transgenic plants; Agrobacterium radiobacter; plasmids; gene expression; Boehmeria nivea; genes; glufosinate; herbicide resistance; culture media; beta-glucuronidase; genetic markers
Abstract:
... A regeneration and transformation protocol for ramie (Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. Co-cultivation of leaf discs with Agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of ...
DOI:
10.1007/BF00232812
PubMed:
24201876

87. Transgenic plants of turfgrass (Agrostis palustris Huds.) from microprojectile bombardment of embryogenic callus

Author:
Zhong, H.; Bolyard, M.G.; Srinivasan, C.; Sticklen, M.B.
Source:
Plant cell reports 1993 v.13 no.1 pp. 1-6
ISSN:
0721-7714
Subject:
Agrostis stolonifera var. palustris; transgenic plants; beta-glucuronidase; enzyme activity; genes; gene expression; gene transfer; callus; embryo (plant)
Abstract:
... Transgenic creeping bentgrass (Agrostis palustris Huds., cv. Pencross; Poaceae) plants have been obtained by microprojectile bombardment of and regeneration from embryogenic calli with a vector designed to deliver the beta-glucuronidase (GUS) gene under the control of rice actin 1 5' regulatory sequences. Southern analysis of polymerase chain reaction (PCR)-amplified and restriction-digested genom ...
DOI:
10.1007/BF00232305
PubMed:
24196173

88. Transgenic root nodules of Vicia hirsuta: a fast and efficient system for the study of gene expression in indeterminate-type nodules

Author:
Quandt, H.J.; Puhler, A.; Broer, I.
Source:
Molecular plant-microbe interactions 1993 v.6 no.6 pp. 699-706
ISSN:
0894-0282
Subject:
genetic transformation; Rhizobium leguminosarum; transgenic plants; Vicia hirsuta; root nodules; Rhizobium rhizogenes; gene expression; histochemistry; reporter genes; nodulation; beta-glucuronidase
DOI:
10.1094/MPMI-6-699

89. Transient and stable expression of gusA fusions with rice genes in rice, barley and perennial ryegrass

Author:
Hensgens, L.A.M.; Bakker, E.P.H.M. de.; Os-Ruygrok, E.P. van.; Rueb, S.; Mark, F. van de.; Maas, H.M. van der.; Veen, S. van der.; Kooman-Gersmann, M.; Hart, L.; Schilperoort, R.A.
Source:
Plant molecular biology 1993 v.22 no.6 pp. 1101-1127
ISSN:
0167-4412
Subject:
Oryza sativa; Hordeum vulgare; Lolium perenne; reporter genes; beta-glucuronidase; recombinant DNA; introns; genetic transformation; transgenic plants; gene expression; exons; messenger RNA; plant proteins; photosystem I; structural genes; cell suspension culture; promoter regions; histochemistry
Abstract:
... Transcriptional and translational fusions were made between the reading frame coding for beta-D-glucuronidase and sequences of either a constitutively expressed rice gene (GOS2) involved in initiation of translation or a light-inducible rice gene (GOS5). The transient expression of the fusions was studied via particle bombardment of seedling tissues of rice, perennial ryegrass and barley. Furtherm ...
DOI:
10.1007/BF00028980
PubMed:
8400127

90. A clearing technique for histochemical location of GUS activity in pollen tubes and ovules of Lycopersicon

Author:
Liedl, B.E.; McCormick, S.; Mutschler, M.A.
Source:
Plant molecular biology reporter 1993 v.11 no.3 pp. 194-201
ISSN:
0735-9640
Subject:
Solanum; pollen tubes; ovules; beta-glucuronidase; enzyme activity; histochemistry; gene expression; laboratory techniques; plant breeding; breeding methods
DOI:
10.1007/BF02669844

91. The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells

Author:
Chibbar, R.N.; Kartha, K.K.; Datla, R.S.S.; Leung, N.; Caswell, K.; Mallard, C.S.; Steinhauer, L.
Source:
Plant cell reports 1993 v.12 no.9 pp. 506-509
ISSN:
0721-7714
Subject:
Hordeum vulgare; genetic transformation; structural genes; introns; actin; alcohol dehydrogenase; Cauliflower mosaic virus; recombinant DNA; reporter genes; beta-glucuronidase; gene expression; transgenic plants; promoter regions; laboratory techniques
Abstract:
... The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcoholdehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intron1 along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli beta- glucuronidase (gus) reporter gene in cultured barley (Hordeum vulgare L) cells. T ...
DOI:
10.1007/BF00236096
PubMed:
24196110

92. The pea plastocyanin promoter directs cell-specific but not full light-regulated expression in transgenic tobacco plants

Author:
Pwee, Keng-Hock; Gray, John C.
Source:
plant journal 1993 v.3 no.3 pp. 437-449
ISSN:
0960-7412
Subject:
beta-glucuronidase; gene expression; peas; plastocyanin; reporter genes; tobacco
Abstract:
... A series of 5′ deletions of the pea plastocyanin gene (petE) promoter fused to the β-glucuronidase (GUS) reporter gene has been examined for expression in transgenic tobacco plants. Strong positive and negative cis-elements which modulate quantitative expression of the transgene in the light and the dark have been detected within the petE promoter. Disruption of a negative regulatory element at −7 ...
DOI:
10.1046/j.1365-313X.1993.t01-26-00999.x

93. The ubiquitous presence of exopolygalacturonase in maize suggests a fundamental cellular function for this enzyme

Author:
Dubald, M.; Barakate, A.; Mandaron, P.; Mache, R.
Source:
plant journal 1993 v.4 no.5 pp. 781-791
ISSN:
0960-7412
Subject:
genetically modified organisms; Zea mays; promoter regions; Nicotiana; polygalacturonase; cell biology; histochemistry; beta-glucuronidase
DOI:
10.1046/j.1365-313X.1993.04050781.x
PubMed:
8275098

94. Abscisic acid promotes novel DNA-binding activity to a desiccation-related promoter of Craterostigma plantagineum

Author:
Nelson, D.; Salamini, F.; Bartels, D.
Source:
plant journal 1994 v.5 no.4 pp. 451-458
ISSN:
0960-7412
Subject:
recombinant DNA; structural genes; binding sites; DNA-binding proteins; reporter genes; abscisic acid; Scrophulariaceae; promoter regions; beta-glucuronidase
DOI:
10.1046/j.1365-313X.1994.5040451.x
PubMed:
8012399

95. Agrobacterium-mediated transformation of apple (Malus X domestica Borkh.): an assessment of factors affecting gene transfer efficiency during early transformation steps

Author:
Bondt, A. de.; Eggermont, K.; Druart, P.; Vil, M. de.; Goderis, I.; Vanderleyden, J.; Broekaert, W.E.
Source:
Plant cell reports 1994 v.13 no.10 pp. 587-593
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; callus; Agrobacterium radiobacter; inoculum density; explants; gene expression; reporter genes; Malus domestica; beta-glucuronidase; leaves
Abstract:
... The factors influencing transfer of an intron-containing beta-glucuronidase gene to apple leaf explants were studied during early steps of an Agrobacterium tumefaciens-mediated transformation procedure. The gene transfer process was evaluated by counting the number of beta-glucuronidase expressing leaf zones immediately after cocultivation, as well as by counting the number of beta-glucuronidase e ...
DOI:
10.1007/BF00234517
PubMed:
24196227

96. Agrobacterium-mediated transformation of white clover using direct shoot organogenesis

Author:
Voisey, C.R.; White, D.W.R.; Dudas, B.; Appleby, R.D.; Ealing, P.M.; Scott, A.G.
Source:
Plant cell reports 1994 v.13 no.6 pp. 309-314
ISSN:
0721-7714
Subject:
genetic transformation; cultivars; tissue culture; Trifolium repens; transgenic plants; Agrobacterium radiobacter; plasmid vectors; gene expression; phosphotransferases (phosphomutases); nucleic acid hybridization; beta-glucuronidase; shoots
Abstract:
... White clover (Trifolium repens L.) plants from the cultivars Grasslands Huia and Grasslands Tahora have been transformed using Agrobacterium-mediated T-DNA transfer. Transgenic plants regenerated directly from cells of the cotyledonary axil. To transform white clover, shoot tips from 3 day old seedlings were co-cultivated with A. tumefaciens strain LBA4404 carrying the plasmid vector pPE64. This v ...
DOI:
10.1007/BF00232627
PubMed:
24193827

97. Amplification and expression of the beta-glucuronidase gene in maize plants by vectors based on maize streak virus

Author:
Shen, W.H.; Hohn, B.
Source:
plant journal 1994 v.5 no.2 pp. 227-236
ISSN:
0960-7412
Subject:
Maize streak virus; Zea mays; genetic code; DNA; gene expression; reporter genes; genetic vectors; beta-glucuronidase; infection
DOI:
10.1046/j.1365-313X.1994.05020227.x

98. Asparaginase gene expression is regulated in a complex spatial and temporal pattern in nitrogen-sink tissues

Author:
Grant, M.; Bevan, M.W.
Source:
plant journal 1994 v.5 no.5 pp. 695-704
ISSN:
0960-7412
Subject:
Lupinus angustifolius; Nicotiana tabacum; asparaginase; recombinant DNA; reporter genes; beta-glucuronidase; gene expression; transgenic plants; leaves; apical meristems; seed development; promoter regions; histochemistry
DOI:
10.1111/j.1365-313X.1994.00695.x

99. Asymmetric somatic hybridization between tomato (Lycopersicon esculentum Mill) and gamma-irradiated potato, (Solanum tuberosum L.): a quantitative analysis

Author:
Schoenmakers, H.C.H.; Wolters, A.M.A.; Haan, A. de.; Saiedi, A.K.; Koorneeff, M.
Source:
Theoretical and applied genetics 1994 v.87 no.6 pp. 713-720
ISSN:
0040-5752
Subject:
restriction fragment length polymorphism; hygromycin B; chloroplasts; protoplast fusion; hybrids; intergeneric hybridization; somatic hybridization; kanamycin; drug resistance; Solanum tuberosum; chloroplast DNA; genomics; reporter genes; cytoplasmic inheritance; gamma radiation; histochemistry; beta-glucuronidase; callus; Solanum lycopersicum var. lycopersicum
Abstract:
... We analyzed 110 asymmetric fusion products, obtained by fusion of hygromycin-resistant tomato protoplasts and gamma-irradiated kanamycin-resistant potato protoplasts that expressed beta-glucuronidase (GUS). The fusion products were selected for resistance to both antibiotics, and were subsequently analyzed for their shoot regeneration potential, GUS activity, expression of two potato isoenzymes, c ...
DOI:
10.1007/BF00222897
PubMed:
24190415

100. Cell-cycle regulation of hydroxyproline-rich glycoprotein HRGPnt3 gene expression during the initiation of lateral root meristems

Author:
Vera, P.; Lamb, C.; Doener, P.W.
Source:
plant journal 1994 v.6 no.5 pp. 717-727
ISSN:
0960-7412
Subject:
genetic transformation; promoter regions; recombinant DNA; beta-glucuronidase; transgenic plants; Rhizobium rhizogenes; cell division; gene expression; histochemistry; Nicotiana tabacum; root meristems; reporter genes; glycoproteins; plant proteins
DOI:
10.1046/j.1365-313X.1994.6050717.x