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You searched for: Subject biotin Remove constraint Subject: biotin Subject term biotin Remove constraint Subject term: biotin Subject term permeability Remove constraint Subject term: permeability Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. Relationship between the Glutamate Production and the Activity of 2-Oxoglutarate Dehydrogenase in Brevibacterium lactofermentum

Author:
Yoshio Kawahara; Keiko Takahashi-Fuke; Eiko Shimizu; Tsuyoshi Nakamatsu; Shigeru Nakamori
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.7 pp. 1109-1112
ISSN:
1347-6947
Subject:
Corynebacterium glutamicum; biotechnology; biotin; cell membranes; enzyme activity; glutamate dehydrogenase; glutamic acid; oxoglutarate dehydrogenase (succinyl-transferring); penicillins; permeability; surfactants
Abstract:
... Enzyme activities of 2-oxoglutarate dehydrogenase complex and glutamate dehydrogenase of wild type Brevibacterium lactofermentum, one of the typical glutamate-producing coryneform bacteria, were investigated by using cells cultured under glutamate-productive and glutamate-non-productive conditions. Significant reduction of the former enzyme activity was observed in the cells under the several glut ...
DOI:
10.1271/bbb.61.1109

2. Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo

Author:
Tarulli, Gerard A.; Meachem, Sarah J.; Schlatt, Stefan; Stanton, Peter G.
Source:
Reproduction 2008 v.135 no.6 pp. 867-877
ISSN:
1470-1626
Subject:
adhesion; adults; biotin; breeding; follicle-stimulating hormone; hamsters; messenger RNA; occludins; permeability; quantitative polymerase chain reaction; spermatogonia; tight junctions; transcription (genetics)
Abstract:
... This study aimed to assess the effect of gonadotrophin suppression and FSH replacement on testicular tight junction dynamics and blood-testis barrier (BTB) organisation in vivo, utilising the seasonal breeding Djungarian hamster. Confocal immunohistology was used to assess the cellular organisation of tight junction proteins and real-time PCR to quantify tight junction mRNA. The effect of tight ju ...
DOI:
10.1530/REP-07-0572
PubMed:
18502899

3. Disruption of blood-testis barrier dynamics in ether-lipid-deficient mice

Author:
Komljenovic, Dorde; Sandhoff, Roger; Teigler, Andre; Heid, Hans; Just, Wilhelm W.; Gorgas, Karin
Source:
Cell and tissue research 2009 v.337 no.2 pp. 281-299
ISSN:
0302-766X
Subject:
Sertoli cells; biotin; lipids; meiosis; mice; permeability; proteins; seminiferous epithelium; spermatocytes; spermiogenesis; tight junctions
Abstract:
... One of the major roles of Sertoli cells is to establish the blood-testis (Sertoli cell) barrier (BTB), which is permanently assembled and disassembled to accommodate the translocation of leptotene spermatocytes from the basal into the adluminal compartment of the seminiferous epithelium and to guarantee completion of meiosis and spermiogenesis. Recently, we have demonstrated spermatogenesis to be ...
DOI:
10.1007/s00441-009-0809-7
PubMed:
19495798

4. IL17A impairs blood–testis barrier integrity and induces testicular inflammation

Author:
Cecilia Valeria Pérez; Eliana Herminia Pellizzari; Selva Beatriz Cigorraga; María Noel Galardo; Munekazu Naito; Livia Lustig; Patricia Verónica Jacobo
Source:
Cell and tissue research 2014 v.358 no.3 pp. 885-898
ISSN:
0302-766X
Subject:
chemoattractants; cell adhesion; macrophages; cytokines; tight junctions; gene expression; biotin; apoptosis; messenger RNA; cell culture; models; in vitro studies; rats; occludins; germ cells; seminiferous tubules; orchitis; inflammation; electrical resistance; Sertoli cells; permeability
Abstract:
... Experimental autoimmune orchitis is a useful model for studying testicular inflammation and germ/immune cell interactions. Th17 cells and their hallmark cytokine IL17A were reported to be involved in the development of autoimmune orchitis. The aim of the present work is to investigate the pathogenic role of IL17A in rat testis. In vitro experiments were performed in order to analyze effects of IL1 ...
DOI:
10.1007/s00441-014-1995-5
PubMed:
25231257

5. Ligand-Directed Active Tumor-Targeting Polymeric Nanoparticles for Cancer Chemotherapy

Author:
Zhong Yinan; Meng Fenghua; Deng Chao; Zhong Zhiyuan
Source:
Biomacromolecules 2014 v.15 no.6 pp. 1955-1969
ISSN:
1526-4602
Subject:
adverse effects; antibodies; biotin; drug formulations; drug therapy; drugs; folic acid; galactose; growth factors; hyaluronic acid; in vivo studies; ligands; models; nanoparticles; neoplasm cells; neoplasms; octreotide; oligonucleotides; permeability; transferrin
Abstract:
... In recent years, polymeric nanoparticles have appeared as a most viable and versatile delivery system for targeted cancer therapy. Various in vivo studies have demonstrated that virus-sized stealth particles are able to circulate for a prolonged time and preferentially accumulate in the tumor site via the enhanced permeability and retention (EPR) effect (so-called “passive tumor-targeting”). The s ...
DOI:
10.1021/bm5003009

6. Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport

Author:
Terai Takuya; Kohno Moe; Boncompain Gaelle; Sugiyama Shigeru; Saito Nae; Fujikake Ryo; Ueno Tasuku; Komatsu Toru; Hanaoka Kenjiro; Okabe Takayoshi; Urano Yasuteru; Perez Franck; Nagano Tetsuo
Source:
Journal of the American Chemical Society 2015 v.137 no.33 pp. 10464-10467
ISSN:
1520-5126
Subject:
X-ray diffraction; binding properties; biotin; dissociation; ligands; permeability; protein transport; screening; streptavidin
Abstract:
... Artificial ligands of streptavidin (ALiS) with association constants of ∼10⁶ M–¹ were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS sys ...
DOI:
10.1021/jacs.5b05672

7. The application of a novel, cell permeable activity-based probe for the detection of cysteine cathepsins

Author:
Caroline S. Hughes; George Shaw; Roberta E. Burden; Christopher J. Scott; Brendan F. Gilmore
Source:
Biochemical and biophysical research communications 2016 v.472 pp. 444-450
ISSN:
0006-291X
Subject:
biomarkers; biotin; cathepsin S; chemistry; cysteine; fluorescence; humans; kidneys; leucine; permeability
Abstract:
... Cysteine cathepsins, such as cathepsin S (CTSS), are implicated in the pathology of a wide range of diseases and are of potential utility as diagnostic and prognostic biomarkers. In previous work, we demonstrated the potency and efficiency of a biotinylated diazomethylketone (DMK)-based activity-based probe (ABP), biotin-PEG-LVG-DMK, for disclosure of recombinant CTSS and CTSS in cell lysates. How ...
DOI:
10.1016/j.bbrc.2016.01.062

8. CD8 T cell-derived perforin aggravates secondary spinal cord injury through destroying the blood-spinal cord barrier

Author:
Zhaoxiang Liu; Hua Zhang; Hong Xia; Baocheng Wang; Renwen Zhang; Qun Zeng; Lingzhi Guo; Kui Shen; BaTa Wang; Yanheng Zhong; Zhizhong Li; Guodong Sun
Source:
Biochemical and biophysical research communications 2019 v.512 no.2 pp. 367-372
ISSN:
0006-291X
Subject:
CD8-positive T-lymphocytes; animal injuries; animal models; biotin; cerebrospinal fluid; cytokines; enzyme-linked immunosorbent assay; flow cytometry; humans; infectious diseases; inflammation; mice; permeability; spinal cord; staining
Abstract:
... Perforin plays an important role in autoimmune and infectious diseases, but its function in immune inflammatory responses after spinal cord injury (SCI) has received insufficient attention. The goal of this study is to determine the influence of perforin after spinal cord injury (SCI) on secondary inflammation. Compared recovery from SCI in perforin knockout (Prf1−/−) and wild-type(WT)mice, WT mic ...
DOI:
10.1016/j.bbrc.2019.03.002

9. Methyl parathion causes genetic damage in sperm and disrupts the permeability of the blood-testis barrier by an oxidant mechanism in mice

Author:
Mayrut Urióstegui-Acosta; Pamela Tello-Mora; María de Jesús Solís-Heredia; José Mario Ortega-Olvera; Belem Piña-Guzmán; Dolores Martín-Tapia; Lorenza González-Mariscal; Betzabet Quintanilla-Vega
Source:
Toxicology 2020 v.438 pp. 152463
ISSN:
0300-483X
Subject:
DNA; DNA damage; Sertoli cells; adults; alpha-tocopherol; antioxidants; atrophy; biotin; developing countries; dose response; histology; immunochemistry; lipid peroxidation; males; mice; oxidants; oxidation; oxidative stress; parathion-methyl; permeability; proteins; seminiferous tubules; sperm quality; spermatogenesis; tight junctions; toxicity
Abstract:
... Methyl parathion (Me-Pa) is an extremely toxic organophosphorus pesticide still used in developing countries. It has been associated with decreased sperm function and fertility and with oxidative and DNA damage. The blood-testis barrier (BTB) is a structure formed by tight junction (TJ) proteins in Sertoli cells and has a critical role in spermatogenesis. We assessed the effect of repeated doses o ...
DOI:
10.1016/j.tox.2020.152463

10. Wu-Zi-Yan-Zong-Wan protects mouse blood-testis barrier from Tripterygium wilfordii Hook. f. multiglycoside-induced disruption by regulating proinflammatory cytokines

Author:
Zhenkun Pan; Yunxiao Gao; Shuang Liu; Zhenghao Ke; Jianqiang Guo; Wenjing Ma; Tianwei Cui; Baoxing Liu; Xiuping Zhang
Source:
Journal of ethnopharmacology 2021 v.280 pp. 114440
ISSN:
0378-8741
Subject:
Sertoli cells; Tripterygium wilfordii; biotin; dose response; histopathology; interleukin-17; interleukin-6; kidneys; male sterility; mice; occludins; permeability; tight junctions; traditional medicine; ultrastructure
Abstract:
... Wu-Zi-Yan-Zong-Wan (WZYZW) is a classical traditonal Chinese herbal formula and a Chinese patent medicine used to treat male infertility. However, the chemical components of WZYZW and its mechanism are not yet fully clarified.The purpose of this study is to observe the effect and underlying mechanism of WZYZW on ameliorating blood-testis barrier (BTB) dysfunction in mice with spermatogenic dysfunc ...
DOI:
10.1016/j.jep.2021.114440

11. Zonula occludens-1 demonstrates a unique appearance in buccal mucosa over several layers

Author:
Keisuke Imafuku; Mayumi Kamaguchi; Ken Natsuga; Hideki Nakamura; Hiroshi Shimizu; Hiroaki Iwata
Source:
Cell and tissue research 2021 v.384 no.3 pp. 691-702
ISSN:
0302-766X
Subject:
biotin; electron microscopy; intestines; mucosa; permeability; research; tight junctions
Abstract:
... Tight junctions (TJs) firmly seal epithelial cells and are key players in the epithelial barrier. TJs consist of several proteins, including those of the transmembrane claudin family and the scaffold zonula occludens (ZO) family. Epithelial tissues are exposed to different conditions: to air in the stratified epithelium of the skin and to liquids in the monolayer of the intestine. The TJs in strat ...
DOI:
10.1007/s00441-021-03425-8

12. Propolis Ameliorates Alcohol-Induced Depressive Symptoms in C57BL/6J Mice by Regulating Intestinal Mucosal Barrier Function and Inflammatory Reaction

Author:
Peng Wang; Peiyu Guo; Yanhui Wang; Xiangyun Teng; Huaqi Zhang; Lirui Sun; Meilan Xue; Hui Liang
Source:
Nutrients 2022 v.14 no.6 pp. -
ISSN:
2072-6643
Subject:
alcohols; biotin; blood serum; brain; dopamine; flora; inflammation; intestines; lipopolysaccharides; liver; models; nerve tissue; occludins; permeability; propolis
Abstract:
... Accumulating evidence points to a critical role of the brain gut axis as an important paradigm for many central nervous system diseases. Recent studies suggest that propolis has obvious neuroprotective properties and functionality in regulating intestinal bacteria flora, hinting at a potential key effect at both terminals of this axis regulation. However, currently no clear evidence confirms the e ...
DOI:
10.3390/nu14061213