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You searched for: Subject calcitonin Remove constraint Subject: calcitonin Subject term calcitonin receptors Remove constraint Subject term: calcitonin receptors
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1. Calcitonin Receptor N-Glycosylation Enhances Peptide Hormone Affinity by Controlling Receptor Dynamics

Author:
Sang-Min Lee; Yejin Jeong; John Simms; Margaret L. Warner; David R. Poyner; Ka Young Chung; Augen A. Pioszak
Source:
Journal of molecular biology 2020 v.432 no.7 pp. 1996-2014
ISSN:
0022-2836
Subject:
asparagine; calcitonin; calcitonin receptors; crystal structure; diabetes; glycosylation; ligands; mass spectrometry; molecular dynamics; osteoporosis; salmon; simulation models
Abstract:
... The class B G protein-coupled receptor (GPCR) calcitonin receptor (CTR) is a drug target for osteoporosis and diabetes. N-glycosylation of asparagine 130 in its extracellular domain (ECD) enhances calcitonin hormone affinity with the proximal GlcNAc residue mediating this effect through an unknown mechanism. Here, we present two crystal structures of salmon calcitonin-bound, GlcNAc-bearing CTR ECD ...
DOI:
10.1016/j.jmb.2020.01.028
PubMed:
32035902
PubMed Central:
PMC7225057

2. Hexapeptide-conjugated calcitonin for targeted therapy of osteoporosis

Author:
Yanpeng Liu; Peng Yu; Xu Peng; Qin Huang; Mingming Ding; Yantao Chen; Ruitao Jin; Jing Xie; Changsheng Zhao; Jianshu Li
Source:
Journal of controlled release 2019 v.304 pp. 39-50
ISSN:
0168-3659
Subject:
adsorption; bone metabolism; calcitonin; calcitonin receptors; femur; hydroxyapatite; hypercalcemia; medicine; models; osteoblasts; osteoclasts; osteopenia; osteoporosis; ovariectomy; patients
Abstract:
... The high level of bone metabolism associated with osteopenia and multifocal skeletal fracture remains a challenging clinical problem in osteoporosis patients. Salmon calcitonin (sCT), as a peptide medicine, is able to inhibit osteoclast activity and stimulate osteoblast growth. However, calcitonin receptors (CTRs) are widely distributed in vivo, limiting the specificity and therapeutic effects. He ...
DOI:
10.1016/j.jconrel.2019.04.042

3. The expression pattern of calcium signaling-related genes during smoltification of Salmo salar in productive conditions

Author:
F.J. Domínguez; J.P. Pontigo; R. Oyarzún; C. Vargas-Lagos; F.J. Morera; L. Vargas-Chacoff
Source:
Comparative biochemistry and physiology 2019 v.231 pp. 20-25
ISSN:
1096-4959
Subject:
Salmo salar; calcitonin; calcitonin receptors; calcium; calcium signaling; calmodulin; gene expression; gene expression regulation; genes; glycolysis; liver; messenger RNA; muscle contraction; muscles; oxidative phosphorylation; parathyroid hormone; parr; quantitative polymerase chain reaction; smoltification; smolts
Abstract:
... Variations in the mRNA expression of hepatic and muscle genes that are related to calcium signaling were analyzed by real-time qPCR in farmed Atlantic salmon (Salmo salar L. 1758) to determine changes in expression between parr and smolt stages. These organs were selected due to their close relationship with calcium signaling and metabolism (e.g., glycolysis, oxidative phosphorylation, muscle cont ...
DOI:
10.1016/j.cbpb.2019.02.002

4. N-Glycosylation of Asparagine 130 in the Extracellular Domain of the Human Calcitonin Receptor Significantly Increases Peptide Hormone Affinity

Author:
Lee Sang-Min; Booe Jason M.; Gingell Joseph J.; Sjoelund Virginie; Hay Debbie L.; Pioszak Augen A.
Source:
Biochemistry 2017 v.56 no.26 pp. 3380-3393
ISSN:
1520-4995
Subject:
Escherichia coli; asparagine; blood glucose; calcitonin; calcitonin receptors; calorimetry; diabetes; fluorescence; food intake; glycosylation; humans; mass spectrometry; molecular models; mutants; osteoporosis; peptide hormones; titration
Abstract:
... The calcitonin receptor (CTR) is a class B G protein-coupled receptor that is activated by the peptide hormones calcitonin and amylin. Calcitonin regulates bone remodeling through CTR, whereas amylin regulates blood glucose and food intake by activating CTR in complex with receptor activity-modifying proteins (RAMPs). These receptors are targeted clinically for the treatment of osteoporosis and di ...
DOI:
10.1021/acs.biochem.7b00256
PubMed:
28614667
PubMed Central:
PMC5551398

5. α-Melanocyte-stimulating hormone directly increases the plasma calcitonin level and involves calcium metabolism in goldfish

Author:
Takahiro Ikari; Yuki Kobayashi; Yoichiro Kitani; Toshio Sekiguchi; Masato Endo; Akira Kambegawa; Kiyoshi Asahina; Atsuhiko Hattori; Yoshiaki Tabuchi; Thumronk Amornsakun; Kanta Mizusawa; Akiyoshi Takahashi; Nobuo Suzuki
Source:
International aquatic research 2018 v.10 no.3 pp. 283-292
ISSN:
2008-4935
Subject:
alpha-melanocyte-stimulating hormone; anesthesia; body weight; bone resorption; calcitonin; calcitonin receptors; calcium; gene expression; goldfish; hormone secretion; messenger RNA; metabolism
Abstract:
... The effects of α-melanocyte-stimulating hormone (α-MSH) on calcium metabolism were examined with goldfish. The scales on the left side of goldfish bodies were removed to allow the regeneration of scales under anesthesia. Thereafter, the influences of α-MSH injection (low dose: 0.1 μg/g body weight; high dose: 1 μg/g body weight) on plasma calcitonin (calcium-regulating hormone) and the calcium con ...
DOI:
10.1007/s40071-018-0206-5

6. Inhibitory effects of two G protein-coupled receptor kinases on the cell surface expression and signaling of the human adrenomedullin receptor

Author:
Kenji Kuwasako; Toshio Sekiguchi; Sayaka Nagata; Danfeng Jiang; Hidetaka Hayashi; Manabu Murakami; Yuichi Hattori; Kazuo Kitamura; Johji Kato
Source:
Biochemical and biophysical research communications 2016 v.470 pp. 894-899
ISSN:
0006-291X
Subject:
calcitonin; calcitonin receptors; cyclic AMP; flow cytometry; gene overexpression; humans; iodine; mutants; phosphotransferases (kinases)
Abstract:
... Receptor activity-modifying protein 2 (RAMP2) enables the calcitonin receptor-like receptor (CLR, a family B GPCR) to form the type 1 adrenomedullin receptor (AM1 receptor). Here, we investigated the effects of the five non-visual GPCR kinases (GRKs 2 through 6) on the cell surface expression of the human (h)AM1 receptor by cotransfecting each of these GRKs into HEK-293 cells that stably expressed ...
DOI:
10.1016/j.bbrc.2016.01.138

7. STC1 interference on calcitonin family of receptors signaling during osteoblastogenesis via adenylate cyclase inhibition

Author:
Silvia R. Terra; João Carlos R. Cardoso; Rute C. Félix; Leo Anderson M. Martins; Diogo Onofre G. Souza; Fatima C.R. Guma; Adelino Vicente M. Canário; Vanessa Schein
Source:
Molecular and Cellular Endocrinology 2015 v.403 pp. 78-87
ISSN:
0303-7207
Subject:
G-proteins; adenylate cyclase; bone formation; calcitonin; calcitonin gene-related peptide; calcitonin receptors; cyclic AMP; forskolin; gene expression; osteoblasts; plasma membrane
Abstract:
... Stanniocalcin 1 (STC1) and calcitonin gene-related peptide (CGRP) are involved in bone formation/remodeling. Here we investigate the effects of STC1 on functional heterodimer complex CALCRL/RAMP1, expression and activity during osteoblastogenesis. STC1 did not modify CALCRL and ramp1 gene expression during osteoblastogenesis when compared to controls. However, plasma membrane spatial distribution ...
DOI:
10.1016/j.mce.2015.01.010

8. Calcitonin receptor 1 (AedaeGPCRCAL1) hindgut expression and direct role in myotropic action in females of the mosquito Aedes aegypti (L.)

Author:
Hyeogsun Kwon; Patricia V. Pietrantonio
Source:
Insect biochemistry and molecular biology 2013 v.43 no.7 pp. 588-593
ISSN:
0965-1748
Subject:
Aedes aegypti; Drosophila melanogaster; Malpighian tubules; RNA interference; Western blotting; blood meal; brain; calcitonin; calcitonin receptors; diuretic hormone; double-stranded RNA; epithelial cells; excretion; females; fluorescent antibody technique; hindgut; insects; muscles; secretion; urine
Abstract:
... In anautogenous mosquitoes such as Aedes aegypti females the calcitonin-like diuretic hormone 31 (DH31) stimulates natriuretic fluid excretion from the Malpighian tubules (MTs) after a blood meal. We previously cloned and functionally characterized AedaeGPCRcal1 from A. aegypti, the ortholog of the Drosophila melanogaster DH31 receptor and immunolocalized it in the MTs. However, localization of th ...
DOI:
10.1016/j.ibmb.2013.03.005
PubMed:
23523607

9. Transient expression of the calcitonin receptor by enteric neurons of the embryonic and early post-natal mouse

Author:
Peter J. Wookey; Kirsty Turner; John B. Furness
Source:
Cell and tissue research 2012 v.347 no.2 pp. 311-317
ISSN:
0302-766X
Subject:
calcitonin; calcitonin receptors; colon; esophagus; ganglia; ileum; mice; milk; nerve tissue; neurons; plexus; pregnancy; villi
Abstract:
... Calcitonin receptor-immunoreactivity (CTR-ir) was found in enteric neurons of the mouse gastrointestinal tract from embryonic day 13.5 (E13.5) to post-natal day 28 (P28). CTR-ir occurred in cell bodies in ganglia of the myenteric plexus extending from the esophagus to the colon and in nerve cells of the submucosal ganglia of the small and large intestines. CTR-ir was also found in vagal nerve trun ...
DOI:
10.1007/s00441-011-1303-6
PubMed:
22271140

10. The role of the ERK1/2 pathway as an alternative to the aging-diminished cyclic AMP pathway in calcitonin-mediated chondrogenesis in human nucleus pulposus

Author:
Wei-Hong Chen; Rong Zeng; Wen-Cheng Lo; Szu-Yu Tina Chen; Tung-Yuan Lai; David F. Williams; Win-Ping Deng
Source:
Biomaterials 2012 v.33 no.33 pp. 8256-8264
ISSN:
0142-9612
Subject:
calcitonin; calcitonin receptors; chondrogenesis; cyclic AMP; humans; phenotype; signal transduction; therapeutics
Abstract:
... Human disc degeneration initiated by aging in the central nucleus pulposus (hNP) is an irreversible process and the recovery has become seriously emerging. In this study, the related mechanisms of calcitonin on the regeneration of hNP and the effects of calcitonin on the age-related alterations were examined. The harvested hNP population was designated as YhNP (from young donor, age <50) and OhNP ...
DOI:
10.1016/j.biomaterials.2012.06.088

11. Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei: Possible physiological roles of the calcitonin family in osmoregulation

Author:
Nobuo Suzuki; Toshio Sekiguchi; Honoo Satake; Kanoko Kato; Yudai Nishiyama; Hideya Takahashi; Janine A. Danks; T. John Martin; Atsuhiko Hattori; Masaki Nakano; Makiko Kakikawa; Sotoshi Yamada; Maho Ogoshi; Susumu Hyodo; Yoko Yamaguchi; Vishwajit S. Chowdhury; Kazuichi Hayakawa; Hisayuki Funahashi; Tatsuya Sakamoto; Yuichi Sasayama
Source:
Gene 2012 v.499 no.2 pp. 326-331
ISSN:
0378-1119
Subject:
Dasyatis; amino acids; animal ovaries; brain; calcitonin; calcitonin receptors; calcium; cartilage; complementary DNA; euryhaline species; flounder; frogs; gall bladder; humans; kidneys; liver; messenger RNA; metabolism; open reading frames; osmoregulation; pancreas; phylogeny; pituitary gland; seawater; spleen; testes
Abstract:
... In cartilaginous fish, two cDNAs encoding calcitonin-family receptors were isolated for the first time from the stingray brain. The open reading frame of one receptor cDNA coded a 525-amino acid protein. The amino acid identity of this receptor to human calcitonin-receptor-like receptor (CRLR) is 64.5%, frog CRLR is 64.7%, and flounder CRLR is 61.2% and this was higher than to human calcitonin rec ...
DOI:
10.1016/j.gene.2012.03.042
PubMed:
22429839

12. Structure–function analysis of amino acid 74 of human RAMP1 and RAMP3 and its role in peptide interactions with adrenomedullin and calcitonin gene-related peptide receptors

Author:
Tao Qi; Kien Ly; David R. Poyner; George Christopoulos; Patrick M. Sexton; Debbie L. Hay
Source:
Peptides 2011 v.32 no.5 pp. 1060-1067
ISSN:
0196-9781
Subject:
amino acid composition; amino acids; calcitonin; calcitonin gene-related peptide; calcitonin receptors; cyclic AMP; enzyme-linked immunosorbent assay; humans; mutants; pharmacology
Abstract:
... The receptors for calcitonin gene-related peptide (CGRP) and adrenomedullin (AM) are complexes of the calcitonin receptor-like receptor (CLR) and receptor activity-modifying proteins (RAMP). The CGRP receptor is a CLR/RAMP1 pairing whereas CLR/RAMP2 and CLR/RAMP3 constitute two subtypes of AM receptor: AM₁ and AM₂, respectively. Previous studies identified Glu74 in RAMP3 to be important for AM bin ...
DOI:
10.1016/j.peptides.2011.03.004

13. Adrenomedullin signalling in bovine Corpus luteum during oestrous cycle, gravidity and induced luteolysis

Author:
Stefanie Schilfarth; Bajram Berisha
Source:
Albanian journal of agricultural sciences 2010 v.9 no.2 pp. 9-17
ISSN:
2218-2020
Subject:
angiogenesis; calcitonin; calcitonin receptors; corpus luteum; cows; estrous cycle; gene expression; luteolysis; messenger RNA; ovariectomy; peptide hormones; pregnancy; quantitative polymerase chain reaction; signal transduction
Abstract:
... Investigations on "nonclassic" regulators of angiogenesis could open new perspectives in understanding angiogenesis under physiological and pathological conditions. A pro-angiogenic effect has been demonstrated for adrenomedullin (AM), it´s co-factor activity-modifying protein 2 (RAMP-2) and the calcitonin receptor-like receptor (CALCLR). This signalling pathway is besides associated with lymphang ...

14. Presence of calcitonin receptors in shell gland of the guineafowl and changes in binding property during an oviposition cycle

Author:
Ogawa, H.; Takahashi, T.; Kuwayama, T.; Kawashima, M.
Source:
Poultry science 2003 v.82 no.8 pp. 1302-1306
ISSN:
0032-5791
Subject:
binding capacity; calcitonin; calcitonin receptors; dissociation; egg shell; endometrium; guineafowl; oviposition; shell gland
Abstract:
... Radioligand binding assays of membrane fraction of the shell gland endometrium of guineafowl revealed the presence of receptor for calcitonin. The equilibrium dissociation constant (Kd) obtained by Scatchard analyses was 0.50 to 1.25 nM in laying birds and 1.12 to 1.19 nM in nonlaying birds. The maximum binding capacity (Bmax) per milligram of protein was 33.1 to 107.5 femtomoles in laying birds a ...
DOI:
10.1093/ps/82.8.1302