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- Author:
- Hogberg, K.A.; Ekberg, I.; Norell, L.; Arnold, S. von.
- Source:
- Canadian journal of forest research = 1998 v.28 no.10 pp. 1536-1545
- ISSN:
- 0045-5067
- Subject:
- tree breeding; cryopreservation; seedlings; embryo (plant); phenology; plant characteristics; height; Picea abies; somatic embryogenesis; agricultural programs and projects; genotype; micropropagation; yields; case studies; tissue culture; Sweden
- Abstract:
- ... Potential for integration of somatic embryogenesis in a breeding programme of Norway spruce (Picea abies (L.) Karst.), and its limitations, were investigated in a case study with an ordinary breeding population. A standard protocol was used for initiation, proliferation, and cryopreservation of embryogenic cultures; maturation of somatic embryos; and plant regeneration. This is a practical necessi ...
- DOI:
- 10.1139/x98-137
- https://doi.org/10.1139/x98-137
- Author:
- Wilkinson, T.; Wetten, A.; Prychid, C.; Fay, M.F.
- Source:
- Annals of botany 2003 v.91 no.1 pp. 65-74
- ISSN:
- 0305-7364
- Subject:
- Cosmos; cryopreservation; endangered species; storage; case studies; root tips; mortality; regrowth; freezing; thawing; meristems; DNA fingerprinting; genetic markers; tissue culture; amplified fragment length polymorphism
- Abstract:
- ... The suitability of cryopreservation for the secure, long-term storage of the rare and endangered species Cosmos atrosanguineus was investigated. Using encapsulation/dehydration of shoot tips in alginate strips, survival rates of up to 100 % and shoot regeneration of up to 35 % were achieved. Light and electron microscopy studies indicated that cellular damage to some regions of the shoot tip durin ...
- DOI:
- 10.1093/aob/mcg009
- PubMed:
- 12495921
- PubMed Central:
- PMC4240354
- https://doi.org/10.1093/aob/mcg009
- Author:
- Newcombe, J.R.; Lichtwark, S.; Wilson, M.C.
- Source:
- Journal of equine veterinary science 2005 v.25 no.2 pp. 70-75
- ISSN:
- 0737-0806
- Subject:
- mares; Standardbred; artificial insemination; spermatozoa; cold storage; cryopreservation; semen; pregnancy rate; male fertility; storage time; storage quality; case studies
- Author:
- Lemos, L.S.; Santos, A.S. de O. dos; Vieira-da-Motta, O.; Texeira, G.N.; Carvalho, E.C.Q. de
- Source:
- Veterinary microbiology 2007 v.125 no.3-4 pp. 350-354
- ISSN:
- 0378-1135
- Subject:
- sheep; sheep diseases; respiratory tract diseases; mycoses; Cryptococcus neoformans; disease detection; case studies; epidemiological studies; pathogen identification; lungs; tissue analysis; cryopreservation; histopathology; yeasts; epithelium; viability; microscopy; differential staining; in vitro culture; risk assessment; disease transmission; zoonoses; Brazil
- Abstract:
- ... Cryptococcosis is considered a zoonosis but is rare in stock animals. In humans it is imputed to be a mortality cause in immunocompromised patients. This case study reports the anatomopathology of respiratory cryptococcosis in ovine and the recuperation and identification of Cryptococcus neoformans var. neoformans from 1-year frozen lung tissue sample. The findings from haematoxylin/eosin staining ...
- DOI:
- 10.1016/j.vetmic.2006.11.028
- https://doi.org/10.1016/j.vetmic.2006.11.028
- Author:
- David Villar; Dubel Balvin; Carlos Giraldo; Miguel Motas; Marta Olivera
- Source:
- Journal of veterinary diagnostic investigation v.22 no.2 pp. 313-315
- ISSN:
- 1040-6387
- Subject:
- Columba livia; wild birds; poisoning; methomyl; mortality; signs and symptoms (animals and humans); cholinesterase; cholinesterase inhibitors; reference standards; enzyme activity; autolysis; brain; air temperature; cryopreservation; enzyme activation; quantitative analysis; case studies; temporal variation
- Abstract:
- ... A mortality event caused by exposure to the carbamate insecticide methomyl was diagnosed in several hundred pigeons fed treated corn kernels in a city park. A cholinesterase inhibitor insecticide was initially suspected based on clinical signs and a significant inhibition (P < 0.05) of brain cholinesterase (ChE) activity compared with normal values for the species. However, brain ChE activity was ...
- DOI:
- 10.1177/104063871002200229
- PubMed:
- 20224101
- https://doi.org/10.1177/104063871002200229
- Author:
- Jepsen, Rebecca J.; Evans, Lawrence E.; Youngs, Curtis R.
- Source:
- Journal of equine veterinary science 2010 v.30 no.11 pp. 651-656
- ISSN:
- 0737-0806
- Subject:
- asses; males; spermatozoa; artificial insemination; pregnancy; cryopreservation; thawing; fertilization (reproduction); cryoprotectants; cholesterol; semen extenders; male fertility; viability; case studies; semen; mares; ovarian follicles; human chorionic gonadotropin; ovulation; cyclodextrins
- DOI:
- 10.1016/j.jevs.2010.10.001
- https://doi.org/10.1016/j.jevs.2010.10.001
- Author:
- Barnicoat, Helen; Cripps, Ryan; Kendon, Jonathan; Sarasan, Viswambharan
- Source:
- In vitro cellular & developmental biology 2011 v.47 no.1 pp. 37-45
- ISSN:
- 1054-5476
- Subject:
- biodiversity; biotechnology; botanical gardens; case studies; conservation programs; cryopreservation; extinction; ferns and fern allies; flora; genetic variation; in vitro culture; indigenous species; islands; micropropagation; seeds; spores
- Abstract:
- ... The importance of in vitro tools to complement other ex situ methods for saving plants from extinction is more relevant than ever before. More than 50% of the world's plant species are endemic to the 34 global biodiversity hotspots (GBHs), each holding at least 1,500 endemic plant species. In addition, a large number of small islands hold a number of endemic species on the brink of extinction. Con ...
- DOI:
- 10.1007/s11627-010-9303-x
- https://doi.org/10.1007/s11627-010-9303-x
- Author:
- Ashmore, Sarah E.; Hamilton, Kim N.; Offord, Catherine A.
- Source:
- In vitro cellular & developmental biology 2011 v.47 no.1 pp. 99-109
- ISSN:
- 1054-5476
- Subject:
- Citrus; Orchidaceae; Syzygium; case studies; cryopreservation; endangered species; epiphytes; ferns and fern allies; flora; germination; indigenous species; plums; rain forests; seed storage; Australia
- Abstract:
- ... This paper highlights recent advances and improved scientific understanding of conservation technologies through selected case studies on threatened plant species indigenous to Eastern Australia. This includes investigations into seed desiccation, storage responses and cryopreservation in rainforest species, particularly the socio-economically important Australian native Citrus spp., Davidsonia sp ...
- DOI:
- 10.1007/s11627-010-9320-9
- https://doi.org/10.1007/s11627-010-9320-9
- Author:
- Kaczmarczyk, Anja; Turner, Shane R.; Bunn, Eric; Mancera, Ricardo L.; Dixon, Kingsley W.
- Source:
- In vitro cellular & developmental biology 2011 v.47 no.1 pp. 17-25
- ISSN:
- 1054-5476
- Subject:
- botanical gardens; case studies; cell membranes; cryopreservation; genotype; germplasm conservation; guidelines; indigenous species; models; nitrogen; temperature; Australia
- Abstract:
- ... Cryogenic storage techniques have been developed and adopted for more than 100 (mainly agricultural) plant species worldwide, and within Australia, at least 30 critically endangered plants have been stored long term using cryogenic approaches. Nevertheless, there are many species that are very difficult to store using current procedures, and organizations involved in plant germplasm conservation ( ...
- DOI:
- 10.1007/s11627-010-9318-3
- https://doi.org/10.1007/s11627-010-9318-3
- Author:
- C. Lessard; L.G. Siqueira; O. D'Amours; R. Sullivan; P. Leclerc; C. Palmer
- Source:
- Theriogenology 2011 v.76 no.5 pp. 891-899
- ISSN:
- 0093-691X
- Subject:
- DNA; Red Angus; acrosome reaction; beef bulls; blastocyst; calcium; case studies; cryopreservation; heifers; in vitro fertilization; parthenogenesis; proteins; semen; sires; sperm concentration; sperm motility; spermatozoa; thawing
- Abstract:
- ... The objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determ ...
- DOI:
- 10.1016/j.theriogenology.2011.04.020
- PubMed:
- 21705058
- https://doi.org/10.1016/j.theriogenology.2011.04.020
- Author:
- Ashmore, S.E.; Drew, R.A.; Kaity, A.
- Source:
- Acta horticulturae 2011 v. no.918 pp. 125-130
- ISSN:
- 0567-7572
- Subject:
- Carica papaya; DNA fingerprinting; DNA methylation; adenosine monophosphate; case studies; cryopreservation; dormancy breaking; field experimentation; flowering; genetic stability; genetic variation; genotype; germination; plant morphology; seed storage; seeds; shoots; storage quality; storage temperature; wild relatives
- Abstract:
- ... Ex situ conservation of Carica papaya and its crop wild relatives has been the subject of ten years of research in our laboratory. This paper summarises the achievements and key findings of this work. Both clonal and seed materials have been investigated to allow storage of elite clonal material as well as maximum genetic diversity. Clonal material from several genotypes has been successfully stor ...
- Author:
- Sershen; Boby Varghese; N.W. Pammenter; Patricia Berjak
- Source:
- Journal of plant physiology 2012 v.169 no.10 pp. 999-1011
- ISSN:
- 0176-1617
- Subject:
- Amaryllis belladonna; Atropa belladonna; Haemanthus; antioxidant activity; antioxidants; case studies; cryopreservation; drying; enzyme activity; enzymes; glycerol; lipid peroxidation; oxidative stress; plant tissues; seeds; sucrose; thiobarbituric acid-reactive substances; viability
- Abstract:
- ... Oxidative stress is a major component of cryoinjury in plant tissues. This study investigated the ability of recalcitrant (i.e. desiccation sensitive) Amaryllis belladonna L. and Haemanthus montanus Baker zygotic embryos to survive cryopreservation, in relation to oxidative stress. The study also investigated whether glycerol cryoprotection promoted embryo post-cryo survival by protecting enzymic ...
- DOI:
- 10.1016/j.jplph.2012.02.016
- PubMed:
- 22591858
- https://doi.org/10.1016/j.jplph.2012.02.016
- Author:
- Keller, E.R.J.; Panis, B.; Engelmann, F.
- Source:
- Acta horticulturae 2012 v. no.961 pp. 35-50
- ISSN:
- 0567-7572
- Subject:
- medicinal plants; European Union; potatoes; environmental hazards; in vitro culture; case studies; germplasm; genetic improvement; Lamiaceae; pests; biodiversity; cryopreservation; nitrogen; breeding; Musa; technology transfer; Allium; seed storage; bananas; France; Belgium
- Abstract:
- ... The existence of modern cultivated plants is a result of interplaying modification by breeding and maintenance of valuable characters by storing the plant biodiversity. Genebanks were established to maintain and provide the crop diversity for use on the long term. Therefore, all possible methods for maintaining plant germplasm are necessary in order to fulfil this purpose for the various types of ...
- DOI:
- 10.17660/ActaHortic.2012.961.2
- https://doi.org/10.17660/ActaHortic.2012.961.2
- Author:
- Aby J Mathew
- Source:
- Cryobiology 2013 v.67 no.3 pp. 412
- ISSN:
- 0011-2240
- Subject:
- biopreservation; blood serum; bone marrow; case studies; cryopreservation; good manufacturing practices; manufacturing; medicine; risk; transportation
- Abstract:
- ... Cellular therapies and regenerative medicine utilize cell and tissue products sourced from blood, bone marrow, and various tissues. The clinical and commercial success of these products is potentially impacted by stability limitations, which include transport of the source material and biopreservation of the final cell or tissue product (either frozen or non-frozen). Traditional isotonic/extracell ...
- DOI:
- 10.1016/j.cryobiol.2013.09.058
- https://doi.org/10.1016/j.cryobiol.2013.09.058
- Author:
- David Frey; Jung Lee
- Source:
- Cryobiology 2013 v.67 no.3 pp. 431
- ISSN:
- 0011-2240
- Subject:
- case studies; compliance; containers; cooling; cryopreservation; engineering; freezers; freezing; good manufacturing practices; ice; manufacturing; models; monitoring; neutropenia; stem cells; storage time; therapeutics
- Abstract:
- ... Cryopreservation is a commonly adopted method for preserving allogeneic cell therapies through long-term storage and distribution to end-users. For clinical and eventually commercial manufacturing of these products, the cooling leg of the cryopreservation cycle is performed within a manufacturing environment that is regulated for Good Manufacturing Practices (GMP). To comply with GMP, the cooling ...
- DOI:
- 10.1016/j.cryobiol.2013.09.122
- https://doi.org/10.1016/j.cryobiol.2013.09.122
- Author:
- André Silva-Pinto; Rita Ferraz; Jorge Casanova; António Sarmento; Lurdes Santos
- Source:
- Medical mycology case reports 2015 v.9 pp. 37-38
- ISSN:
- 2211-7539
- Subject:
- Candida parapsilosis; allografting; case studies; cryopreservation; endocarditis; fluconazole; intravenous injection; ischemia; morbidity; mortality; mycology; patients
- Abstract:
- ... Candida endocarditis is a rare infection associated with high mortality and morbidity. There are still some controversies about Candida endocarditis treatment, especially about the treatment duration. We report a case of a Candida parapsilosis endocarditis that presented as a lower limb ischemia. The patient was surgically treated with a cryopreserved homograft aortic replacement. We used intraven ...
- DOI:
- 10.1016/j.mmcr.2015.07.004
- PubMed:
- 26288749
- PubMed Central:
- PMC4539163
- https://doi.org/10.1016/j.mmcr.2015.07.004
- Author:
- E Hu; Thunshun Warren Liao; Terrence R Tiersch
- Source:
- Aquaculture research 2015 v.46 no.2 pp. 432-445
- ISSN:
- 1355-557X
- Subject:
- Ictalurus furcatus; Ictalurus punctatus; aquaculture; automation; case studies; computer simulation; computer software; cryopreservation; decision making; equipment; fish; fish industry; fish production; germplasm; hybrids; planning; simulation models; spawning; spermatozoa; work schedules; Wisconsin
- Abstract:
- ... Emerging commercial‐level technology for aquatic sperm cryopreservation has not been modelled by computer simulation. Commercially available software (ARENA, Rockwell Automation, Inc. Milwaukee, WI) was applied to simulate high‐throughput sperm cryopreservation of blue catfish (Ictalurus furcatus) based on existing processing capabilities. The goal was to develop a simulation model suitable for pr ...
- DOI:
- 10.1111/are.12192
- PubMed:
- 25580079
- PubMed Central:
- PMC4285714
- https://doi.org/10.1111/are.12192
- Author:
- P. H. Purdy; C. S. Wilson; S. F. Spiller; H. D. Blackburn
- Source:
- Reproduction, fertility, and development 2016 v.28 no.8 pp. 1072-1078
- ISSN:
- 1031-3613
- Subject:
- USDA; animal genetic resources; case studies; databases; germplasm; germplasm conservation; infrastructure; issues and policy; phenotype; sheep; United States
- Abstract:
- ... There is adequate infrastructure in the US to identify and acquire germplasm from the major beef and dairy cattle and swine breeds. However, when we venture outside these species, the same tasks become more difficult because of a lack of breed associations, databases that include genotypic and phenotypic data and low numbers of animals. Furthermore, acquisition of germplasm from non-cattle and non ...
- DOI:
- 10.1071/RD15399
- https://doi.org/10.1071/RD15399
- Author:
- Flavia Pizzi; Federica Turri; Teresa M. Gliozzi; Gustavo Gandini
- Source:
- Italian journal of animal science 2016 v.15 no.2 pp. 207-210
- ISSN:
- 1828-051X
- Subject:
- animal science; blood; case studies; cattle; cost analysis; cryopreservation; genes; goats; semen; sheep
- Abstract:
- ... Scientific and technical reports on farm animal genetic resources (AnGR) cryoconservation activities, and related costs, are needed to optimise conservation programmes. In this paper, we presented the recent Italian AnGR gene banking development, including the creation of the first regional animal cryobank, the ‘Lombardia Farm Animal Genetic Resources Cryobank’ (LABank). In order to provide indica ...
- DOI:
- 10.1080/1828051X.2016.1153406
- https://doi.org/10.1080/1828051X.2016.1153406
- Author:
- Namrata Kumari; Mukesh Kumar Gupta; Raghubansh Kumar Singh
- Source:
- Cryobiology 2016 v.73 no.2 pp. 232-239
- ISSN:
- 0011-2240
- Subject:
- Anabaena; Oocystis; algae; beta-mercaptoethanol; case studies; cost effectiveness; cryopreservation; genetic resources; glutathione; microorganisms; viability; vitrification
- Abstract:
- ... Vitrification offers a cost-effective solution for the preservation and management of genetic resources with, low-cost international movement of selected genetic materials and for long-term maintenance of stable stocks of a wide variety of microorganisms. However, its success is limited by the wide range of algal species. Here, we report a simple open encapsulation-vitrification protocol of cryopr ...
- DOI:
- 10.1016/j.cryobiol.2016.07.005
- https://doi.org/10.1016/j.cryobiol.2016.07.005
- Author:
- Natalie Obroucheva; Irina Sinkevich; Snejana Lityagina
- Source:
- Tree physiology 2016 v.36 no.9 pp. 1127-1150
- ISSN:
- 1758-4469
- Subject:
- Aesculus hippocastanum; air; antioxidant activity; body water; carbohydrate metabolism; case studies; cryopreservation; dormancy; germination; hormonal regulation; longevity; recalcitrant seeds; tree physiology; trees
- Abstract:
- ... Recalcitrant seeds are typical of some tropical and subtropical trees. Their post-shedding life activity proceeds in humid air and wet litter. They are desiccation sensitive and, for this reason, have a short life span and need some special procedures for cryopreservation. This review is devoted to the post-shedding life strategy of recalcitrant seeds, which includes the maintenance of high hydrat ...
- DOI:
- 10.1093/treephys/tpw037
- https://doi.org/10.1093/treephys/tpw037
- Author:
- Ellie Merrett Wade; Jayanthi Nadarajan; Xiangyun Yang; Daniel Ballesteros; Weibang Sun; Hugh W. Pritchard
- Source:
- Plant diversity 2016 v.38 no.5 pp. 209-220
- ISSN:
- 2468-2659
- Subject:
- Angiospermae; case studies; cryopreservation; ex situ conservation; extinction; ferns and fern allies; habitat destruction; in situ conservation; risk; seeds; species diversity; spore germination; spores; storage; storage quality; threatened species; China
- Abstract:
- ... Approximately one fifth of the world's plants are at risk of extinction. Of these, a significant number exist as populations of few individuals, with limited distribution ranges and under enormous pressure due to habitat destruction. In China, these most-at-risk species are described as ‘plant species with extremely small populations’ (PSESP). Implementing conservation action for such listed speci ...
- DOI:
- 10.1016/j.pld.2016.09.002
- PubMed:
- 30159468
- PubMed Central:
- PMC6112217
- https://doi.org/10.1016/j.pld.2016.09.002
- Author:
- E. R. J. Keller; A. Senula
- Source:
- Acta horticulturae 2016 no.1143 pp. 35-44
- ISSN:
- 0567-7572
- Subject:
- Allium; antibiotics; bulbs; case studies; cloves; cryopreservation; differential scanning calorimetry; endophytes; explants; garlic; gene banks; inflorescences; microbial load; nitrogen; plant development; pollen; shoot tips; water content
- Abstract:
- ... The genus Allium has been one of the main research objects of the Gatersleben institute for many years. Due to the high proportion of accessions that had been collected from the original sites a comprehensive living collection was established. Maintenance of living material was performed with a spectrum of methods, namely field culture, seed and in vitro storage, and cryopreservation. Vegetatively ...
- DOI:
- 10.17660/ActaHortic.2016.1143.6
- https://doi.org/10.17660/ActaHortic.2016.1143.6
- Author:
- Takekiyo Takahiro; Ishikawa Yuka; Yoshimura Yukihiro
- Source:
- Journal of physical chemistry 2017 v.121 no.32 pp. 7614-7620
- ISSN:
- 1520-5207
- Subject:
- Fourier transform infrared spectroscopy; aqueous solutions; case studies; cattle; circular dichroism spectroscopy; cryopreservation; cryoprotectants; cytochrome c; denaturation; dialysis; dimethyl disulfide; dimethyl sulfoxide; heart; ionic liquids; nitrates; solubilization; thiocyanates; ultraviolet-visible spectroscopy
- Abstract:
- ... Aqueous ionic liquid (IL) solutions form a glassy state at 77 K over a wide concentration of ILs. They have potential as novel cryopreservation/refolding solvents for proteins. However, even if proteins in glass-forming concentrations of ILs are preserved at 77 K, the recovery of activity and the structure of the proteins after cryopreservation are still unclear. To achieve high recovery of protei ...
- DOI:
- 10.1021/acs.jpcb.7b05158
- https://doi.org/10.1021/acs.jpcb.7b05158
25. Automated image processing as an analytical tool in cell cryopreservation for bioprocess development
- Author:
- Sarah Gretzinger; Stefanie Limbrunner; Jürgen Hubbuch
- Source:
- Bioprocess and biosystems engineering 2019 v.42 no.5 pp. 665-675
- ISSN:
- 1615-7591
- Subject:
- automation; biological specimen banks; biomass; biopharmaceuticals; bioprocessing; case studies; cell lines; cryopreservation; freeze-thaw cycles; freezing; image analysis; islets of Langerhans; models; storage temperature; thawing; therapeutics
- Abstract:
- ... The continuous availability of cells with defined cell characteristics represents a crucial issue in the biopharmaceutical and cell therapy industry. Here, development of cell banks with a long-term stability is essential and ensured by a cryopreservation strategy. The strategy needs to be optimized for each cell application individually and usually comprises controlled freezing, storage at ultra- ...
- DOI:
- 10.1007/s00449-019-02071-3
- https://doi.org/10.1007/s00449-019-02071-3
- Author:
- Jian-Ling Guo; Yan-Hong Yu; Jian-Wen Zhang; Zhi-Min Li; Yong-Hong Zhang; Sergei Volis
- Source:
- Biodiversity and conservation 2019 v.28 no.6 pp. 1533-1548
- ISSN:
- 0960-3115
- Subject:
- Ottelia; amplified fragment length polymorphism; aquatic plants; case studies; climate; conservation areas; cryopreservation; environmental degradation; gene flow; genetic analysis; genetic similarity; genetic variation; geographical distribution; lakes; models; niches; phenotype; ponds; rivers; seeds; threatened species
- Abstract:
- ... Knowledge of the extent and structure of genetic diversity and a species’ ecological niche are integral to formulating management plans for the conservation of threatened species. This is particularly important for aquatic organisms, which are especially vulnerable to human-induced environmental degradation and experience rapid global decline. To develop a proper conservation strategy for a threat ...
- DOI:
- 10.1007/s10531-019-01740-9
- https://doi.org/10.1007/s10531-019-01740-9
27. Managing plant genetic resources using low and ultra-low temperature storage: a case study of tomato
- Author:
- Dariusz Kulus
- Source:
- Biodiversity and conservation 2019 v.28 no.5 pp. 1003-1027
- ISSN:
- 0960-3115
- Subject:
- alleles; biochemical pathways; case studies; cooling; cryopreservation; cultivars; fecundity; freezers; frost; gene banks; genetic drift; genetic erosion; humidity; liquids; markets; nitrogen; plant genetic resources; pollen; protocols; seeds; shoot tips; somaclonal variation; storage time; temperature; tissues; tomatoes; vegetable crops; vitrification
- Abstract:
- ... Ex situ preservation of plant genetic resources is essential. Tomato is one of the most important vegetable crops on the market. However, the genetic diversity of the clade is limited and suffering from genetic erosion phenomenon. Genebanks experience alleles loss on regeneration of small samples, genetic drift, and somaclonal variation in in vitro cultures. Therefore, the development of more effi ...
- DOI:
- 10.1007/s10531-019-01710-1
- https://doi.org/10.1007/s10531-019-01710-1
- Author:
- XuXu Yang; Elena Popova; Mukund R. Shukla; Praveen K. Saxena
- Source:
- In vitro cellular & developmental biology 2019 v.55 no.4 pp. 392-402
- ISSN:
- 1054-5476
- Subject:
- Hypericum perforatum; aluminum foil; case studies; cryopreservation; genotype; gibberellic acid; glycerol; in vitro studies; medicinal plants; plant age; regrowth; root tips; sucrose; vitrification
- Abstract:
- ... In this study, an effective root-based cryopreservation method was developed for Hypericum perforatum L., an important medicinal species, using in vitro plants. A systematic approach was applied to determine effective combinations of protocol steps such as preculture, osmoprotection, vitrification solution treatment, and unloading, followed by protocol optimization using a single-factor approach. ...
- DOI:
- 10.1007/s11627-019-09999-x
- https://doi.org/10.1007/s11627-019-09999-x
- Author:
- Maria Cristina Budani; Gian Mario Tiboni
- Source:
- Cryobiology 2020 v.97 pp. 242-244
- ISSN:
- 0011-2240
- Subject:
- blastomeres; case studies; cryobiology; cryopreservation; embryo transfer; females; in vitro fertilization; patients; pregnancy
- Abstract:
- ... Embryo cryopreservation represents a central procedure in in-vitro fertilization (IVF) programs. This report documents a Case of a successful pregnancy following the replacement of embryos that had to be re-vitrified due to the forced cancellation of the frozen embryo-transfer (FET).The 37- year-old patient was referred to our Assisted Reproductive Technology (ART) unit for idiopathic infertility ...
- DOI:
- 10.1016/j.cryobiol.2020.08.009
- https://doi.org/10.1016/j.cryobiol.2020.08.009
- Author:
- Ronald L. Sandler; Lisa Moses; Samantha M. Wisely
- Source:
- Biological conservation 2021 v.257 pp. 109118
- ISSN:
- 0006-3207
- Subject:
- Mustela nigripes; case studies; cryopreservation; endangered species; ethics; ferrets; fibroblasts; gene pool; genetic load; genetic rescue; genome
- Abstract:
- ... Genetic rescue is a management tool used to decrease genetic load and increase fitness. The technique relies on the addition of unique genomes into a population's gene pool, but when no living candidates are available for translocation, preserved genetic resources may be an option. Biobanks are a source of cryopreserved material, including fibroblasts which have the potential to be used as source ...
- DOI:
- 10.1016/j.biocon.2021.109118
- https://doi.org/10.1016/j.biocon.2021.109118
- Author:
- Meijing Sun; Yu Guo; Naiqian Zhao; Shuo Zhang; Kun Pei; Chuanxin Qin
- Source:
- Marine environmental research 2022 v.176 pp. 105588
- ISSN:
- 0141-1136
- Subject:
- Acanthopagrus; case studies; cryopreservation; environmental DNA; ethanol; fish; genes; research
- Abstract:
- ... The development of a standardized eDNA detection process is the primary step in improving the accuracy and efficiency of eDNA detection. In this study, primers and probes with high specificity were selected to identify the COI gene of Acanthopagrus latus. Through experiments on the influence of different water quantities, methods of water sample preservation and water bathing times on the result o ...
- DOI:
- 10.1016/j.marenvres.2022.105588
- https://doi.org/10.1016/j.marenvres.2022.105588