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- Author:
- L. R. Devireddy; R. Raghavan; S. Ramachandran; M. S. Shaila
- Source:
- Archives of virology 1999 v.144 no.6 pp. 1241-1247
- ISSN:
- 0304-8608
- Subject:
- antiserum; cell fusion; chickens; erythrocytes; giant cells; glycoproteins; hemolysis; immunoaffinity chromatography; peste des petits ruminants; viruses
- Abstract:
- ... The fusion glycoprotein (F protein) of paramyxoviruses plays a vital role in virus-induced cytopathology. To explore the role of the F protein in peste des petits ruminants virus (PPRV)-induced cytopathology, the F protein of PPRV was purified by immunoaffinity chromatography. The purified F protein, when incubated with chicken erythrocytes, caused lysis suggesting that PPRV F protein is a hemolys ...
- DOI:
- 10.1007/s007050050583
- PubMed:
- 10446657
- https://doi.org/10.1007/s007050050583
- Author:
- Ferreira, Helena L.; Pilz, Daniela; Mesquita, Ligia G.; Cardoso, Tereza
- Source:
- Avian pathology 2003 v.32 no.4 pp. 413-417
- ISSN:
- 0307-9457
- Subject:
- Infectious bronchitis virus; RNA; cell fusion; chicken eggs; chickens; cytopathogenicity; electron microscopy; fluorescence; fluorescent antibody technique; kidney cells; reverse transcriptase polymerase chain reaction; titration; virus replication; viruses
- Abstract:
- ... The susceptibility of the chicken embryo related (CER) cell line to infectious bronchitis virus (IBV M41) was characterized after five consecutive passages in CER cells. Virus replication was monitored by cytopathic effect observation, electron microscopy, indirect immunofluorescence, and reverse transcription polymerase chain reaction (RT-PCR). At 96 h post-infection (p.i.), the cytopathic effect ...
- DOI:
- 10.1080/0307945031000121167
- https://doi.org/10.1080/0307945031000121167
- Author:
- Jun-Jie Chen; Jin-Qing Jiang
- Source:
- Food and agricultural immunology 2013 v.24 no.3 pp. 331-344
- ISSN:
- 1465-3443
- Subject:
- acetonitrile; cell fusion; chickens; ciprofloxacin; correlation; cross reaction; detection limit; ducks; enzyme-linked immunosorbent assay; geese; inhibitory concentration 50; mass spectrometry; methanol; monitoring; monoclonal antibodies; muscles; pH; screening; sodium chloride
- Abstract:
- ... This study presents the generation of monoclonal antibodies (mAbs) through cell fusion procedure and the development of mAb-based indirect competitive ELISA (icELISA) for detecting ciprofloxacin (CIP) residue in poultry samples. Under optimal experimental conditions, this assay exhibited a working range of 0.05–83.2 ng/mL, with the half maximal inhibitory concentration (IC ₅₀) and the limit of det ...
- DOI:
- 10.1080/09540105.2012.689817
- https://doi.org/10.1080/09540105.2012.689817
- Author:
- Yuya Okuzaki; Shunsuke Kidani; Hidenori Kaneoka; Shinji Iijima; Ken-ichi Nishijima
- Source:
- Bioscience, biotechnology, and biochemistry 2017 v.81 no.5 pp. 914-921
- ISSN:
- 1347-6947
- Subject:
- adults; biotechnology; cell fusion; chickens; fibroblasts; genetic vectors; pH; pathogenicity; reverse transcriptase polymerase chain reaction; transmembrane proteins
- Abstract:
- ... Interferon-inducible transmembrane protein (IFITM) family proteins are antivirus factors. In the present study, we examined the expression pattern of chicken IFITM10 using quantitative reverse transcription-polymerase chain reaction. In adult chickens, IFITM10 levels were markedly lower than those of IFITM3, which exhibits antivirus activity. On the other hand, IFITM10 was expressed in levels simi ...
- DOI:
- 10.1080/09168451.2016.1274639
- https://doi.org/10.1080/09168451.2016.1274639
- Author:
- Zhaozhou Li; Yao Wang; Daomin Li; Xiujin Chen; Zhili Li; Hongli Gao; Li Cao; Songbiao Li; Yuze Hou
- Source:
- RSC advances 2017 v.7 no.57 pp. 36072-36080
- ISSN:
- 2046-2069
- Subject:
- animal tissues; antigens; cell fusion; chickens; coccidiosis; cost effectiveness; cross reaction; drug residues; enzyme-linked immunosorbent assay; high performance liquid chromatography; inhibitory concentration 50; liver; monoclonal antibodies; muscles; risk; screening
- Abstract:
- ... Ethopabate (ETP) is a coccidiostat that is frequently used to prevent and treat coccidiosis in chickens. Illegal use and abuse of ETP can lead to drug residues in edible animal tissues, which present a potential health risk to consumers. To rapidly monitor ETP residues, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed that has simple ...
- DOI:
- 10.1039/c6ra20736g
- https://doi.org/10.1039/c6ra20736g
- Author:
- Lei Zhuang; Beat Trueb
- Source:
- Archives of biochemistry and biophysics 2017 v.625-626 pp. 54-64
- ISSN:
- 0003-9861
- Subject:
- Echinoidea; amino acids; cell fusion; chickens; chimerism; fish; genes; giant cells; humans; hydrophobicity; mice; mutagenesis; receptors
- Abstract:
- ... FGFRL1 is a transmembrane receptor that can induce the fusion of CHO cells to multinucleated syncytia. This cell fusion activity has been attributed to the extracellular Ig3 domain of the receptor. We investigated how the fusogenic activity evolved during the evolution of animals. We found that the Ig3 domain from humans, mice, chicken and fish stimulates fusion of CHO cells, while the Ig3 domain ...
- DOI:
- 10.1016/j.abb.2017.06.002
- https://doi.org/10.1016/j.abb.2017.06.002
- Author:
- Lingling Guo; Liguang Xu; Shanshan Song; Liqiang Liu; Hua Kuang
- Source:
- Food and agricultural immunology 2018 v.29 no.1 pp. 458-469
- ISSN:
- 1465-3443
- Subject:
- antibiotics; antigens; ascites; breast meat; cell fusion; chicken meat; chickens; cross reaction; detection limit; eggs; gold; immunization; immunoaffinity chromatography; immunoglobulin G; maduramicin; monoclonal antibodies; rapid methods
- Abstract:
- ... Following the steps of antigen synthesis, immunization, cell fusion, ascites preparation and purification, a maduramicin (MD) monoclonal antibody (mAb) was produced. This MD-mAb demonstrated a 50% inhibition concentration value of 3.75 ng/ml, an affinity constant of 3.70 × 10¹⁰ l/mol, and the isotype was IgG3. The MD-mAb has no cross-reactivity with other polyether antibiotics. Using this MD-mAb, ...
- DOI:
- 10.1080/09540105.2017.1401045
- https://doi.org/10.1080/09540105.2017.1401045
- Author:
- Muhammad Asif Rasheed; Abdur Rahman Ansari; Awais Ihsan; Muhammad Tariq Navid; Shahid ur-Rehman; Sohail Raza
- Source:
- Microbial pathogenesis 2018 v.116 pp. 91-99
- ISSN:
- 0882-4010
- Subject:
- adsorption; antiviral agents; bioinformatics; cattle; cell fusion; cell membranes; chickens; glycoproteins; herpes simplex; horses; humans; prediction; swine; viruses
- Abstract:
- ... Glycoprotein B (gB), C (gC) and D (gD) of herpes simplex virus are implicated in virus adsorption and penetration. The gB, gC and gD are glycoproteins for different processes of virus binding and attachment to the host cells. Moreover, their expression is necessary and sufficient to induce cell fusion in the absence of other glycoproteins. Egress of herpes simplex virus (HSV) and other herpes viru ...
- DOI:
- 10.1016/j.micpath.2018.01.020
- https://doi.org/10.1016/j.micpath.2018.01.020
- Author:
- Jun Dong; Junqing Fan; Yuanyuan Wang; Qinxue Zhang; Yingli Yang; Yunqiang Jia; Fan Ming; Xue Zhang; Rong Yao; Shuyun Li; Lihua Zhang; Ran Li; Gaoyuan Xu
- Source:
- Analytical biochemistry 2019 v.572 pp. 52-57
- ISSN:
- 0003-2697
- Subject:
- Influenza A virus; antibody detection; blood serum; cell fusion; chickens; cross reaction; ducks; enzyme-linked immunosorbent assay; epitopes; geese; genes; hemagglutination; hemagglutinins; humans; hybridomas; monoclonal antibodies; pigeons; poultry industry; quails; rapid methods; China
- Abstract:
- ... Since 2013, the H7 subtype avian influenza virus (AIV-H7) has seriously endangered human life and health, and has had a serious impact on the poultry industry in China. A competitive enzyme-linked immunosorbent assay (C-ELISA) which detects the antibody for AIV-H7 was developed, basing on a monoclonal antibody (mAb) against the neutralizing epitopes on hemagglutinin (HA)gene. Twelve hybridoma cell ...
- DOI:
- 10.1016/j.ab.2019.02.024
- https://doi.org/10.1016/j.ab.2019.02.024
- Author:
- Renata Erbert Contriciani; Fernanda Cristina da Veiga; Mariana Juliani do Amaral; Bianca Gazieri Castelucci; Lizandra Maia de Sousa; Marcelo Bispo de Jesus; Sílvio Roberto Consonni; Carla Beatriz Collares-Buzato; Cláudia Mermelstein; Susanne Dietrich; Lúcia Elvira Alvares
- Source:
- Comparative biochemistry and physiology 2021 v.256 pp. 110645
- ISSN:
- 1096-4959
- Subject:
- bioinformatics; cell cycle; cell fusion; chickens; chicks; computer simulation; gene expression; humans; mice; muscle development; muscles; myoblasts; protein structure; protein synthesis; separation; skeletal muscle
- Abstract:
- ... Vertebrate skeletal muscle development and repair relies on the precise control of Wnt signaling. Dact1 (Dapper/Frodo) is an important modulator of Wnt signaling, interacting with key components of the various Wnt transduction pathways. Here, we characterized Dact1 mRNA and protein expression in chicken and mouse fetal muscles in vivo and during the differentiation of chick primary and mouse C2C12 ...
- DOI:
- 10.1016/j.cbpb.2021.110645
- https://doi.org/10.1016/j.cbpb.2021.110645
- Author:
- Masako Tada; Ayaka Hayashi; Yumi Asano; Musashi Kubiura-Ichimaru; Takamasa Ito; Miho Yoshii; Hiroshi Kimura; Yoichi Matsuda; Mitsuo Oshimura
- Source:
- Genes & genomics 2021 v.43 no.3 pp. 269-280
- ISSN:
- 1976-9571
- Subject:
- B-cell lymphoma; DNA; DNA demethylation; DNA methylation; DNA methyltransferase; cell differentiation; cell fusion; chickens; chromatin; epigenetics; gastrulation; genomics; humans; mice; oxygenases
- Abstract:
- ... BACKGROUND: DNA methylation is a significant epigenetic modification that is evolutionarily conserved in various species and often serves as a repressive mark for transcription. DNA methylation levels and patterns are regulated by a balance of opposing enzyme functions, DNA methyltransferases, DNMT1/3A/3B and methylcytosine dioxygenases, TET1/2/3. In mice, the TET enzyme converts DNA cytosine meth ...
- DOI:
- 10.1007/s13258-021-01046-7
- https://doi.org/10.1007/s13258-021-01046-7
- Author:
- Xiangyu Huang; Jingwen Huang; Guihu Yin; Yiqin Cai; Mengli Chen; Jianing Hu; Xiuli Feng
- Source:
- Viruses 2022 v.14 no.6 pp. -
- ISSN:
- 1999-4915
- Subject:
- B-lymphocytes; Influenza A virus; antibody specificity; avian influenza; cell fusion; chickens; epitopes; fluorescent antibody technique; genes; hybridomas; monoclonal antibodies; poultry industry; prediction; public health
- Abstract:
- ... Avian Influenza (AI) caused by the H9N2 subtype of the avian influenza virus (AIV) poses a serious threat to both the poultry industry and to public health safety. NP is one of the major structural proteins in influenza viruses. B-cell determinants located on NP proteins have attracted increasing attention. In this study, based on the NP sequence of the H9N2 (A/chicken/Shandong/LY1/2017) strain, t ...
- DOI:
- 10.3390/v14061172
- https://doi.org/10.3390/v14061172