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1. Reproduction and life history of the red alga galaxaura oblongata (nemaliales, galaxauraceae)¹

Author:
William H. Magruder
Source:
Journal of phycology 1984 v.20 no.3 pp. 402-409
ISSN:
0022-3646
Subject:
Galaxaura; cell fusion; gametophytes; life history; pericarp; reproduction; thallus
Abstract:
... Culture and morphological studies showed that Galaxaura oblongata (Ellis et Solander) Lamouroux has a triphasic life history with conspicuous gametophytes and small filamentous tetrasporophytes. Development of male and female reproductive structures is very similar and both begin with the enlargement of a terminal cell of a filament branch occupying a normal vegetative position within the apical p ...
DOI:
10.1111/j.0022-3646.1984.00402.x

2. An in vitro system for adhesion and fusion of maize gametes

Author:
Faure, J.E.; Digonnet, C.; Dumas, C.
Source:
Science 1994 v.263 no.5153 pp. 1598-1600
ISSN:
0036-8075
Subject:
fertilization (reproduction); Zea mays; germ cells; adhesion; cell fusion
Abstract:
... The development of in vitro fertilization systems in flowering plants is important for understanding and controlling the mechanisms of fertilization. Here a method is described in which isolated maize gametes fuse. In a medium containing 5 mM calcium chloride, sperm and egg cells adhere for several minutes and then fuse within 10 seconds. The method is specific to male-female gamete pairs and resu ...
DOI:
10.1126/science.263.5153.1598
PubMed:
17744790

3. Transfection of Sendai virus F gene cDNA with mutations at its cleavage site and HN gene cDNA into COS cells induces cell fusion

Author:
Taira, H.; Sato, T.; Segawa, H.; Chiba, M.; Katsumata, T.; Iwasaki, K.
Source:
Archives of virology 1995 v.140 no.1 pp. 187-194
ISSN:
0304-8608
Subject:
cell fusion; nucleotide sequences; amino acid sequences; viral proteins; transfection; giant cells
Abstract:
... In contrast to the wild type Sendai virus fusion protein (F), a mutated F to possess a cleavage site similar to that of virulent Newcastle disease virus F could be cleaved by proteases present in COS cells. When mutated F and hemagglutinin-neuraminidase (HN) were coexpressed at the cell surface, syncytium formation was observed. ...
DOI:
10.1007/BF01309734
PubMed:
7646344

4. Aspects of fusion combining ability of dihaploid Solanum tuberosum L.: influence of the cytoplasm

Author:
Frei, U.; Stattmann, M.; Lossl, A.; Wenzel, G.
Source:
Potato research 1998 v.41 no.2 pp. 155-162
ISSN:
0014-3065
Subject:
protoplast fusion; haploidy; phenotype; somatic hybridization; genome; Solanum tuberosum; mitochondria; genotype; cytoplasmic inheritance; cell fusion
DOI:
10.1007/BF02358438

5. Channel catfish virus: interaction with the cytoskeleton

Author:
Pope, R.K.; Scheetz, R.W.
Source:
Journal of fish diseases 1998 v.21 no.6 pp. 433-442
ISSN:
0140-7775
Subject:
Ictalurid herpesvirus 1; Ictalurus punctatus; cell fusion; giant cells; microfilaments; microtubules; paclitaxel; virus replication; viruses
Abstract:
... The infection of channel catfish ovarian cells with channel catfish virus results in the redistribution of all cytoskeletal elements during virus-induced syncytium formation. Actin filaments are normally arranged as large stress fibres, even during early stages of infection, but become redistributed as densely staining regions near the centre of the syncytium in latent infection. Microtubules and ...
DOI:
10.1046/j.1365-2761.1998.00129.x

6. Characterization of haemagglutinin-esterase protein (HE) of murine corona virus DVIM by monoclonal antibodies

Author:
H. Kasai; E. Morita; K. Hatakeyama; K. Sugiyama
Source:
Archives of virology 1998 v.143 no.10 pp. 1941-1948
ISSN:
0304-8608
Subject:
Murine coronavirus; cell fusion; diarrhea; epitopes; glycoproteins; mice; monoclonal antibodies; viruses
Abstract:
... We analyzed the characteristics of seven monoclonal antibodies (mAbs) raised against purified HE (hemagglutinin-esterase) glycoprotein of the murine coronavirus DVIM (diarrhea virus of infant mice). Immunocrossreaction of these mAbs with JHM and/or MHV-S suggest that antigenic epitopes of HE of DVIM are similar to those of JHM and/or MHV-S. Four mAbs (1b4, 3Aff28, 4c19, 10b7), designated as group ...
DOI:
10.1007/s007050050431
PubMed:
9856082

7. Recombination of mitochondrial DNA without selection pressure among compatible strains of the Aspergillus niger species aggregate

Author:
Toth, B.; Hamari, Z.; Ferenczy, L.; Varga, J.; Kevei, F.
Source:
Current genetics 1998 v.33 no.3 pp. 199-205
ISSN:
0172-8083
Subject:
restriction fragment length polymorphism; oligomycin; Aspergillus niger; mitochondrial DNA; strain differences; genetic recombination; cell fusion; drug resistance
DOI:
10.1007/s002940050327
PubMed:
9508794

8. Tomato flower abnormalities induced by low temperatures are associated with changes of expression of MADS-box genes

Author:
Lozano, R.; Angosto, T.; Gomez, P.; Payan, C.; Capel, J.; Huijser, P.; Salinas, J.; Martinez-Zapater, J.M.
Source:
Plant physiology 1998 v.117 no.1 pp. 91-100
ISSN:
0032-0889
Subject:
homeotic genes; meristems; developmental stages; flowers; transcription (genetics); gene expression; plant morphology; abnormal development; temperature; cell fusion; ultrastructure; Solanum lycopersicum var. lycopersicum
Abstract:
... Flower and fruit development in tomato (Lycopersicon esculentum Mill.) were severely affected when plants were grown at low temperatures, displaying homeotic and meristic transformations and alterations in the fusion pattern of the organs. Most of these homeotic transformations modified the identity of stamens and carpels, giving rise to intermediate organs. Complete homeotic transformations were ...

9. The fusion of sperm cells and the function of male germ unit (MGU) of tobacco (Nicotiana tabacum L.)

Author:
Tian, H.Q.; Russell, S.D.
Source:
Sexual plant reproduction 1998 v.11 no.3 pp. 171-176
ISSN:
0934-0882
Subject:
ovules; germ cells; calcium; pollen; pollen tubes; Nicotiana tabacum; plasma membrane; cell fusion
DOI:
10.1007/s004970050137

10. The hemagglutinin of recent measles virus isolates induces cell fusion in a marmoset cell line, but not in other CD46-positive human and monkey cell lines, when expressed together with the F protein

Author:
K. Tanaka; M. Xie; Y. Yanagi
Source:
Archives of virology 1998 v.143 no.2 pp. 213-225
ISSN:
0304-8608
Subject:
giant cells; humans; monkeys; Measles morbillivirus; transfection; hemagglutinins; cytopathogenicity; genes; cell fusion
Abstract:
... Measles virus (MV) is efficiently isolated from patients with measles by using B95a cells, a marmoset B cell line. Recent wild-type MV strains isolated using B95a cells did not produce cytopathic effects in any of CD46⁺ primate cell lines examined (except B95a cells), nor did they induce downregulation of CD46. Transfection of the hemagglutinin (H) and fusion (F) genes of the Edmonston strain of M ...
DOI:
10.1007/s007050050281
PubMed:
9541608

11. A thermoresistant strain of feline immunodeficiency virus (FIV) with an altered cytopathic effect and a restricted cell tropism

Author:
C. Alix; F. Koehren; J.-P. Martin; A. Kirn; J. Braunwald
Source:
Archives of virology 1998 v.143 no.11 pp. 2093-2107
ISSN:
0304-8608
Subject:
Feline immunodeficiency virus; bone marrow; cats; cell fusion; cytopathogenicity; genes; giant cells; glycoproteins; kidney cells; macrophages; mutation; phenotype; temperature; viruses
Abstract:
... A thermoresistant strain, designated m41, of feline immunodeficiency virus (FIV) was selected after 31 successive passages of chronically infected IRC4 cells at 41 °C. The wild-type virus (wt) which served as a control was cultivated the same number of times at 37 °C. In Crandell feline kidney cells (CrFK), the replication of m41 was similar at 37 °C and 41 °C, whereas wt multiplied only at 37 °C. ...
DOI:
10.1007/s007050050446
PubMed:
9856096

12. Amino acid substitutions at position 481 differently affect the ability of the measles virus hemagglutinin to induce cell fusion in monkey and marmoset cells co-expressing the fusion protein

Author:
M.-F. Xie; K. Tanaka; N. Ono; H. Minagawa; Y. Yanagi
Source:
Archives of virology 1999 v.144 no.9 pp. 1689-1699
ISSN:
0304-8608
Subject:
asparagine; amino acid substitution; gene expression regulation; monkeys; Measles morbillivirus; tyrosine; hemagglutinins; mutants; site-directed mutagenesis; cell fusion
Abstract:
... The hemagglutinin (H) protein of the measles virus (MV) Edmonston strain induced cell fusion in Cos (monkey) and B95a (marmoset) cells, when co-expressed with the fusion (F) protein, whereas the H protein of the wild-type KA strain induced fusion in B95a cells, but not in Cos cells. Asparagine residue at position 481 of the KA H protein was replaced by various amino acids through site-directed mut ...
DOI:
10.1007/s007050050697
PubMed:
10542019

13. Hypovirulence detected in Brazilian isolates of Cryphonectria cubensis

Author:
Van Zyl, L.M.; Wingfield, M.J.; Alfenas, A.C.; Crous, P.W.
Source:
Plant pathology 1999 v.48 no.2 pp. 267-272
ISSN:
0032-0862
Subject:
strain differences; hyphae; crossing; cell fusion; RNA; virulence; Brazil
DOI:
10.1046/j.1365-3059.1999.00334.x

14. Nuclear and cell fusion cause polyploidy in the megagametophyte of common cypress, Cupressus cempervirens L

Author:
El Maataoui, M.; Pichot, C.
Source:
Planta 1999 v.208 no.3 pp. 345-351
ISSN:
0032-0935
Subject:
mitosis; metabolism; chromosome number; histology; DNA; cell walls; genome; flow cytometry; seed development; polyploidy; endosperm; gametocytes; Cupressus sempervirens; cytochemistry; embryogenesis; cell fusion; nutrient reserves
Abstract:
... In common cypress, Cupressus sempervirens L., the megagametophyte persists in mature seeds as a polyploid endosperm containing cells with even and odd series of DNA contents: 1C, 2C, 3C, 4C, 5C etc., where C is the amount of DNA in the haploid genome. In this study, cytometrical, histological and cytochemical investigations were performed in order to determine the behavior of megagametophyte nucle ...
DOI:
10.1007/s004250050568

15. The fusion protein of peste des petits ruminants virus is a hemolysin

Author:
L. R. Devireddy; R. Raghavan; S. Ramachandran; M. S. Shaila
Source:
Archives of virology 1999 v.144 no.6 pp. 1241-1247
ISSN:
0304-8608
Subject:
antiserum; cell fusion; chickens; erythrocytes; giant cells; glycoproteins; hemolysis; immunoaffinity chromatography; peste des petits ruminants; viruses
Abstract:
... The fusion glycoprotein (F protein) of paramyxoviruses plays a vital role in virus-induced cytopathology. To explore the role of the F protein in peste des petits ruminants virus (PPRV)-induced cytopathology, the F protein of PPRV was purified by immunoaffinity chromatography. The purified F protein, when incubated with chicken erythrocytes, caused lysis suggesting that PPRV F protein is a hemolys ...
DOI:
10.1007/s007050050583
PubMed:
10446657

16. Morphological changes in homeotic cytoplasmic male-sterile carrots combined with fertile cytoplasm by asymmetrical cell fusion

Author:
Yamamoto, T.; Nakajima, Y.; Oeda, K.
Source:
Plant cell reports 2000 v.19 no.4 pp. 363-370
ISSN:
0721-7714
Subject:
cytoplasmic male sterility; plant development; hybrids; restriction mapping; phenotype; mitochondrial DNA; fecundity; Daucus carota; flowers; plant morphology; cytoplasm; genomics; cytoplasmic inheritance; cell fusion
DOI:
10.1007/s002990050741

17. Effect of protein supplementation, O2 concentration and co-culture on the development of embryos produced by nuclear transfer using cultured cumulus cells in Hanwoo (Korean cattle)

Author:
Im, G.S.; Yang, B.S.; Park, S.J.; Im, S.K.; Yang, B.C.; Yi, Y.J.; Park, C.S.
ISSN:
1011-2367
Subject:
ionophores; pregnancy rate; protein supplements; feeds; oocytes; cattle; culture media; blastocyst; cytoplasm; cumulus oophorus; embryogenesis; cell fusion; oxygen; metaphase; embryo transfer; tissue culture
DOI:
10.5713/ajas.2001.1260

18. Nuclear transfer in loach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion

Author:
Hongtuo, F.; Chingjiang, W.
Source:
Aquaculture research 2001 v.32 no.4 pp. 267-275
ISSN:
1355-557X
Subject:
Cobitidae; cell fusion; oocytes; cell division; cell culture; culture media; dosage; ultraviolet radiation; genetic techniques and protocols
Abstract:
... To study nuclear transfer in the loach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 degrees C in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained ...
DOI:
10.1046/j.1365-2109.2001.00555.x

19. The effects of donor cell type and culture medium on in vitro development of domestic cat embryos reconstructed by nuclear transplantation

Author:
Fahrudin, M.; Otoi, T.; Karja, N.W.K.; Murakami, M.; Suzuki, T.
ISSN:
1011-2367
Subject:
oocytes; cats; morula; biotechnology; cell division; blastocyst; cytoplasm; cumulus oophorus; cell fusion; embryo culture; culture media; oviducts
DOI:
10.5713/ajas.2001.1057

20. Molecular characterization of the nuclear and cytoplasmic genomes of intergeneric diploid plants from cell fusion between Microcitrus papuana and Rough lemon

Author:
Liu, J.H.; Pang, X.M.; Cheng, Y.J.; Meng, H.J.; Deng, X.X.
Source:
Plant cell reports 2002 v.21 no.4 pp. 327-332
ISSN:
0721-7714
Subject:
restriction fragment length polymorphism; protoplasts; citrus fruits; protoplast fusion; intergeneric hybridization; diploidy; chemical constituents of plants; mitochondrial DNA; genome; embryo (plant); polymerase chain reaction; greenhouse production; chloroplast DNA; plant morphology; cytoplasm; genomics; Citrus jambhiri; cell fusion; repetitive sequences
Abstract:
... Simple-sequence-repeat (SSR) and PCR-RFLP were employed to characterize the nuclear and cytoplasmic genomes of intergeneric diploid plants derived from symmetric fusion between Microcitrus papuana Swingle and Rough lemon (Citrus jambhiri Lush). Three out of five SSR primers distinguished the fusion parents from each other and the regenerated plants showed band profiles completely identical to the ...
DOI:
10.1007/s00299-002-0517-1