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1. Development and validation of a GC-FID method for the analysis of short chain fatty acids in rat and human faeces and in fermentation fluids

Author:
Serena Scortichini; Maria Chiara Boarelli; Stefania Silvi; Dennis Fiorini
Source:
Journal of chromatography 2020 v.1143 pp. 121972
ISSN:
1570-0232
Subject:
acetic acid; acidification; chemical species; derivatization; ethyl ether; feces; fermentation; flame ionization; hexanoic acid; humans; intestinal microorganisms; metabolites; mice; polyethylene glycol; propionic acid; quantitative analysis; rats
Abstract:
... Short-chain fatty acids (SCFAs) are gut microbiota metabolites recognized for their beneficial effects on the host organism. In this study, a simple and rapid sample preparation method combined to SCFAs analysis by direct injection and gas chromatography coupled with flame ionization detection (GC-FID), for the determination and quantification of eight SCFAs (acetic, propionic, i-butyric, butyric, ...
DOI:
10.1016/j.jchromb.2020.121972