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You searched for: Subject chickens Remove constraint Subject: chickens Subject term bacterial antigens Remove constraint Subject term: bacterial antigens
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1. Evaluation of SEF14 fimbrial dot blot and flagellar Western blot tests as indicators of Salmonella enteritidis infection in chickens

Author:
Cooper, G.L.; Thorns, C.J.
Source:
Veterinary record 1996 v.138 no.7 pp. 149-153
ISSN:
0042-4900
Subject:
chickens; cross reaction; immunoblotting; fimbriae; lipopolysaccharides; serodiagnosis; Salmonella Enteritidis; flagellum; enzyme-linked immunosorbent assay; bacterial antigens; genetic variation
DOI:
10.1136/vr.138.7.149
PubMed:
8677603

2. Immunisation of chickens to reduce intestinal colonisation with Campylobacter jejuni

Author:
Widders, P.R.; Perry, R.; Muir, W.I.; Husband, A.J.; Long, K.A.
Source:
British poultry science 1996 v.37 no.4 pp. 765-778
ISSN:
0007-1668
Subject:
chickens; intestinal microorganisms; immunoglobulin G; immunoglobulin M; antibody formation; application methods; cecum; microbial contamination; meat carcasses; immunoglobulin A; bacterial antigens; immunization; flagellin; Campylobacter jejuni
Abstract:
... 1. Systemic and intestinal antibody titres were measured in chickens following subcutaneous, intraperitoneal (IP), oral (po) and combined IP/po administration of antigen, in soluble, emulsified or microparticulate form. Antigens tested included keyhole limpet haemocyanin (KLH), killed Campylobacter jejuni whole cells and purified campylobacter flagellin protein. 2. The effect of immunisation with ...
DOI:
10.1080/00071669608417906
PubMed:
8894221

3. Impact of alternate-day feeding cycles on immune and antibacterial responses of White Leghorn chicks

Author:
Boa-Amponsem, K.; Yang, A.; Praharaj, N.K.; Dunnington, E.A.; Gross, W.B.; Siegel, P.B.
Source:
Journal of applied poultry research 1997 v.6 no.2 pp. 123-127
ISSN:
1056-6171
Subject:
chickens; chicks; poultry feeding; ad libitum feeding; restricted feeding; antibody formation; Escherichia coli; bacterial antigens; erythrocytes; body weight
DOI:
10.1093/japr/6.2.123

4. Campylobacteri jejuni flagellin-Escherichia coli LT-B fusion protein

Author:
Meinersmann, R.J.; Khoury, C.A.
Source:
United States Department of Agriculture patents 1999 no.5,888,810 pp. -
Subject:
enterotoxins; chickens; bacterial colonization; patents; recombinant fusion proteins; disease prevention; poultry diseases; animal pathogenic bacteria; amino acid sequences; bacterial antigens; bacterial toxins; nucleotide sequences; USDA; flagellin; Campylobacter jejuni; Escherichia coli; United States
Abstract:
... A fusion protein which comprises the B subunit of the labile toxin (LT-B) of E. coli and part of the flagellin (flaA) protein of C. jejuni is antigenic and is useful for decreasing colonization in chickens by Campylobacter species. The protein is produced by E. coli cells, transformed by the plasmid pBEB into which DNA sequences encoding the novel protein have been introduced. ...
Handle:
10113/6969

5. Use of chicken egg-yolk antibodies against K88+ fimbrial antigen for quantitative analysis of enterotoxigenic Escherichia coli (ETEC) K88+ by a sandwich ELISA

Author:
Kim, J.W.; Jin, L.Z.; Cho, S.H.; Marquardt, R.R.; Frohlich, A.A.; Baidoo, S.K.
Source:
Journal of the science of food and agriculture 1999 v.79 no.11 pp. 1513-1518
ISSN:
0022-5142
Subject:
bacteria; Escherichia coli; bacterial antigens; fimbriae; egg yolk; antibodies; chickens; serodiagnosis; feces; sampling; piglets; enzyme-linked immunosorbent assay
Abstract:
... An enzyme-linked immunosorbent sandwich assay (ELISA) has been developed to detect and quantify both the K88+ fimbrial antigen and the concentration of bacterial cells in fecal and other samples using anti-fimbrial chicken egg-yolk and anti-fimbrial rabbit serum antibodies. The assay has a sensitivity of 40 ng ml(-1) for K88+ fimbrial antigen and 10(7) CFU ml(-1) for intact cells and cell homogena ...
DOI:
10.1002/(SICI)1097-0010(199908)79:11<1513::AID-JSFA396>3.0.CO;2-0

6. In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium

Author:
Lee, E.N.; Sunwoo, H.H.; Menninen, K.; Sim, J.S.
Source:
Poultry science 2002 v.81 no.5 pp. 632-641
ISSN:
0032-5791
Subject:
chickens; cross reaction; egg yolk composition; surface antigens; antibody formation; Salmonella Enteritidis; electron microscopy; Salmonella Typhimurium; immunoglobulins; egg yolk; bacterial antigens; binding sites; immunization; powders; antibacterial properties
Abstract:
... Chicken egg yolk antibody (IgY) raised against Salmonella enteritidis or Salmonella typhimurium was found in highly specific activity levels by ELISA. S. enteritidis- and S. typhimurium-specific IgY powder, prepared by freeze-drying the egg yolk water-soluble fraction, contained 15.5 and 10.0% of specific IgY, respectively. Anti-S. enteritidis IgY cross-reacted 55.3% with S. typhimurium. The cross ...
DOI:
10.1093/ps/81.5.632
PubMed:
12033412

7. Stable Biocompatible Adjuvants — a New Type of Adjuvant Based on Solid Lipid Nanoparticles: A Study on Cytotoxicity, Compatibility and Efficacy in Chicken

Author:
Carsten Olbrich; Rainer Helmut Müller; Kerstin Tabatt; Oliver Kayser; Christoph Schulze; Rüdiger Schade
Source:
ATLA. Alternatives to laboratory animals v.30 no.4 pp. 443-458
ISSN:
0261-1929
Subject:
animal use replacement; adjuvants; paraffin wax; Mycoplasma bovis; bacterial antigens; antibodies; acylglycerols; drug delivery systems; humans; breast muscle; cytotoxicity; glycerol; granuloma; surfactants; bioassays; egg yolk; animal use refinement; immunization; histopathology; chickens; antibody formation; injection site; laboratory animals; particle size; cultured cells; solvents; fibroblasts
Abstract:
... A new type of adjuvant was tested for its ability to initiate antibody production in chickens, and its cellular and tissue compatibility were assessed. The stable biocompatible adjuvants tested are based on surface-modified solid lipid nanoparticles (SLNs), made from paraffin or biodegradable glyc-erides, and are simply admixed to the antigens before administration. The tissue-damaging potency of ...
DOI:
10.1177/026119290203000407
PubMed:
12234249

8. Antigen-specific lymphocyte proliferation and interleukin production in chickens immunized with killed Salmonella enteritidis vaccine or experimental subunit vaccines

Author:
Okamura, M.; Lillehoj, H.S.; Raybourne, R.B.; Babu, U.; Heckert, R.
Source:
Avian diseases 2003 v.47 no.4 pp. 1331-1338
ISSN:
1938-4351
Subject:
chickens; Salmonella Enteritidis; inactivated vaccines; interleukin-2; flagellum; interleukin-6; outer membrane proteins; bacterial antigens; vaccination; lymphocyte proliferation; subunit vaccines
Abstract:
... Lymphocyte proliferation and interleukin (IL)-2 and IL-6 levels in serum were measured as indicators of cell-mediated immunity after immunization of chickens with a commercial killed Salmonella enteritidis (SE) vaccine or experimental subunit vaccines of crude protein (CP) extract or the outer membrane protein (OMP). Significantly increased proliferative responses to SE flagella, but not lipopolys ...
Handle:
10113/47118
DOI:
10.1637/6096

9. Cell-mediated immune responses to a killed Salmonella enteritidis vaccine: lymphocyte proliferation, T-cell changes and interleukin-6 (IL-6), IL-1, IL-2, and IFN-γ production

Author:
Okamura, M.; Lillehoj, H.S.; Raybourne, R.B.; Babu, U.S.; Heckert, R.A.
Source:
Comparative immunology, microbiology, and infectious diseases 2004 v.27 no.4 pp. 255-272
Subject:
chickens; biosynthesis; lipopolysaccharides; interleukin-1; Salmonella Enteritidis; T-lymphocytes; nitric oxide; interleukin-6; interleukin-2; flagellum; inactivated vaccines; bacterial antigens; splenocytes; vaccination; lymphocyte proliferation; animal age; interferons
Abstract:
... Two experimental approaches were used to investigate the immunological responses of chickens to a commercial killed Salmonella enteritidis (SE) vaccine. In the first, the effects of host age on antigen-specific proliferative responses and cytokine production were examined. Compared with non-vaccinated controls, 4-wk-old vaccinated chickens showed higher proliferation to SE LPS and flagella. The ly ...
Handle:
10113/42597
DOI:
10.1016/j.cimid.2003.12.001

10. Genetic detoxification and adjuvant-activity retention of Escherichia coli enterotoxin LT

Author:
Vasserman, Y.; Pitcovski, J.
Source:
Avian pathology 2006 v.35 no.2 pp. 134-140
ISSN:
0307-9457
Subject:
Escherichia coli; enterotoxins; vaccine adjuvants; toxicity; immunostimulants; microbial genetics; genes; nucleotide sequences; amino acid sequences; oral administration; chickens; antibody formation; field experimentation; bacterial antigens
Abstract:
... Intensive effort has been invested in the search for new effective vaccines that can be conveniently administered, with minimal handling of birds. Heat-labile enterotoxin (LT) of Escherichia coli H10407 is known as a powerful adjuvant for injection and oral administration. However, to use the toxin as an immunostimulator in animals, its toxicity must be neutralized. To this aim, we modified the LT ...
DOI:
10.1080/03079450600597873
PubMed:
16595306

11. Immune Response to Recombinant Escherichia coli Iss Protein in Poultry

Author:
Lynne, A.M.; Foley, S.L.; Nolan, L.K.
Source:
Avian diseases 2006 v.50 no.2 pp. 273-276
ISSN:
0005-2086
Subject:
chickens; Escherichia coli; pathogenicity; animal pathogenic bacteria; bacterial infections; microbial genetics; bacterial proteins; genes; immune response; recombinant fusion proteins; humoral immunity; lesions (animal); bacterial antigens
Abstract:
... Colibacillosis accounts for significant losses to the poultry industry, and control efforts are hampered by limited understanding of the mechanisms used by avian pathogenic Escherichia coli (APEC) to cause disease. We have found that the presence of the increased serum survival gene (iss) is strongly associated with APEC but not with commensal E. coli, making iss, and the protein it encodes (Iss), ...
DOI:
10.1637/7441-092105R.1
PubMed:
16863080

12. Profile of Toll-like receptor expressions and induction of nitric oxide synthesis by Toll-like receptor agonists in chicken monocytes

Author:
He, H.; Genovese, K.J.; Nisbet, D.J.; Kogut, M.H.
Source:
Molecular immunology 2006 v.43 no.7 pp. 783-789
Subject:
chickens; oligonucleotides; Staphylococcus aureus; transmembrane proteins; messenger RNA; biosynthesis; nitric oxide; monocytes; Salmonella Enteritidis; Salmonella Typhimurium; gene expression; bacterial antigens; double-stranded RNA; receptors; agonists
Abstract:
... Toll-like receptors (TLRs) play a major role in the innate immune system for initial recognition of microbial pathogens and pathogen associated components. Nitric oxide (NO) is generated in immune cells in response to microbial stimulation and is involved in pathogenesis and control of infection. We used RT-PCR analysis to examine the TLR expression profile on chicken monocytes and demonstrated th ...
Handle:
10113/278
DOI:
10.1016/j.molimm.2005.07.002
PubMed:
16098593

13. Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA

Author:
Hsu, Y.M.; Shieh, H.K.; Chen, W.H.; Shiang, J.H.; Chang, P.C.
Source:
Veterinary microbiology 2007 v.122 no.3-4 pp. 280-289
ISSN:
0378-1135
Subject:
chickens; protective effect; hemagglutination; in vivo studies; Avibacterium paragallinarum; serotypes; poultry diseases; bacterial antigens; hemagglutinins; genes; vaccination; vaccine development; antigenic variation; subunit vaccines; bacterial infections; genetic recombination
Abstract:
... Inactivated vaccines of Avibacterium paragallinarum provide protection and reduce the economic losses caused by infectious coryza. However, inactivated bacterins provide protection only against the Page serovars included in the vaccine. In this study, we investigated the immunological properties of a functional recombinant haemagglutinin protein (rHagA) derived from a Taiwan isolate strain A9 as t ...
DOI:
10.1016/j.vetmic.2007.01.014

14. Mycoplasma synoviae lipoprotein MSPB, the N-terminal part of VlhA haemagglutinin, induces secretion of nitric oxide, IL-6 and IL-1β in chicken macrophages

Author:
Lavric, M.; Bencina, D.; Kothlow, S.; Kaspers, B.; Narat, M.
Source:
Veterinary microbiology 2007 v.121 no.3-4 pp. 278-287
ISSN:
0378-1135
Subject:
chickens; poultry diseases; respiratory tract diseases; joint diseases; mycoplasmosis; Mycoplasma synoviae; cell culture; immune response; interleukin-1; interleukin-6; secretion; nitric oxide; biochemical mechanisms; immunomodulators; bacterial antigens; bacterial proteins; lipoproteins; hemagglutinins; in vitro studies; macrophages
Abstract:
... Mycoplasma synoviae is a major pathogen of chickens and turkeys, causing respiratory disease and infectious synovitis. M. synoviae haemagglutinin VlhA is an abundant surface-exposed lipoprotein and immunodominant antigen. Post-translational cleavage of VlhA generates two proteins, MSPB and MSPA. MSPB, the amino-terminal end of VlhA, is a lipoprotein of about 40-50 kDa but can appear in truncated f ...
DOI:
10.1016/j.vetmic.2006.12.005

15. Serological Responses of Chickens to Low Challenge Doses of Mycoplasma Synoviae

Author:
Kleven, S.H.; Ferguson-Noel, N.; Raviv, Z.; Wooten, R.; Laibinis, V.
Source:
Avian diseases 2007 v.51 no.3 pp. 738-743
ISSN:
0005-2086
Subject:
chickens; serodiagnosis; hemagglutination inhibition test; respiratory tract diseases; strain differences; poultry diseases; mycoplasmosis; disease transmission; enzyme-linked immunosorbent assay; bacterial antigens; immune response; sentinel animals; Mycoplasma synoviae
Abstract:
... Groups of eight chickens were challenged with 10-fold dilutions of one of two strains of Mycoplasma synoviae (MS); each challenge group contained two noninfected sentinels. Both strains were highly efficient in colonizing the respiratory tract with challenge doses as low as 76 and 24 color-changing units/bird. Infection spread rapidly (within 7 days) to sentinels, while uninfected control chickens ...
DOI:
10.1637/0005-2086(2007)51[738:SROCTL]2.0.CO;2
PubMed:
17992935

16. Identification and characterization of haemagglutinin epitopes of Avibacterium paragallinarum serovar C

Author:
Noro, Taichi; Oishi, Eiji; Kaneshige, Takahiro; Yaguchi, Kazuhiko; Amimoto, Katsuhiko; Shimizu, Mitsugu
Source:
Veterinary microbiology 2008 v.131 no.3-4 pp. 406-413
ISSN:
0378-1135
Subject:
epitopes; chickens; Avibacterium paragallinarum; inactivated vaccines; disease prevention; serotypes; recombinant proteins; poultry diseases; enzyme-linked immunosorbent assay; bacterial antigens; hemagglutinins; monoclonal antibodies; immune response; vaccination; vaccine development; bacterial infections; protective effect
Abstract:
... The objectives of this study were to identify haemagglutinin (HA) epitopes of Avibacterium paragallinarum serovar C that are capable of eliciting haemagglutination inhibition (HI) antibody, and to investigate their immunogenic role. Three conformational epitopes were detected on HA by blocking ELISA and immuno-dot blot analysis using a panel of five monoclonal antibodies (MAbs) with HI activity, d ...
DOI:
10.1016/j.vetmic.2008.04.010
PubMed:
18514440

17. In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

Author:
Amaral, José Araujo; De Franco, Milene Tino; Zapata-Quintanilla, Lucy; Carbonare, Solange Barros
Source:
Veterinary research communications 2008 v.32 no.4 pp. 281-290
ISSN:
0165-7380
Subject:
Shiga toxin; bacterial antigens; microbial growth; protective effect; animal diseases; pathotypes; antigen-antibody reactions; human diseases; serotypes; egg yolk; immunization; in vitro studies; Escherichia infections; antibacterial properties; chickens; immunoglobulin Y; food pathogens; Escherichia coli O157; anti-infective agents
Abstract:
... IgY is a chicken egg yolk antibody which has been used for treatment and prophylaxis of gastrointestinal infections. Our aim was to verify if IgY obtained from chickens immunized with EPEC O111, STEC O111 and STEC O157 is able to show in vitro reactivity and biological activity towards the three bacteria. IgY was obtained from eggs laid before and after immunization with each bacterium. The prepar ...
DOI:
10.1007/s11259-007-9029-3
PubMed:
18071921

18. Siderophore Receptor IroN Is an Important Protective Antigen Against Salmonella Infection in Chickens

Author:
Kaneshige, Takahiro; Yaguchi, Kazuhiko; Ohgitani, Toshiaki
Source:
Avian diseases 2009 v.53 no.4 pp. 563-567
ISSN:
0005-2086
Subject:
chickens; Salmonella Enteritidis; chelating agents; Salmonella Typhimurium; iron; vaccine adjuvants; bacterial antigens; membrane proteins; bacterial proteins; immunization; siderophores; receptors; subunit vaccines; mortality
Abstract:
... In iron-limiting environments, Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium synthesize and secrete several types of siderophore to trap trivalent ferric ions; these bacteria then express siderophore receptors called iron-regulated outer membrane proteins (IROMPs). In this study, we experimentally reproduced iron-limiting environments using a divalent metal ch ...
DOI:
10.1637/8925-051309-Reg.1
PubMed:
20095157

19. Rapid serodiagnosis with the use of surface plasmon resonance imaging for the detection of antibodies against major surface protein A of Mycoplasma synoviae in chickens

Author:
Oh, Kiseok; Lee, Semi; Seo, Jayoung; Lee, Dongwoo; Kim, Taejung
Source:
Canadian journal of veterinary research 2010 v.74 no.1 pp. 71-74
ISSN:
0830-9000
Subject:
chickens; mycoplasmosis; poultry diseases; Mycoplasma synoviae; animal pathogenic bacteria; antibody detection; serodiagnosis; diagnostic techniques; surface plasmon resonance; antibodies; major surface glycoproteins; bacterial antigens; hemagglutinins; lipoproteins; post-translational modification; enzyme-linked immunosorbent assay; accuracy
PubMed:
20357963
PubMed Central:
PMC2801316

20. Efficacy of Soluble Recombinant FliC Protein from Salmonella enterica Serovar Enteritidis as a Potential Vaccine Candidate Against Homologous Challenge in Chickens

Author:
Okamura Masashi; Matsumoto Wakako; Seike Fumio; Tanaka Yuuya; Teratani Chie; Tozuka Maki; Kashimoto Takashige; Takehara Kazuaki; Nakamura Masayuki; Yoshikawa Yasuhiro
Source:
Avian diseases 2012 v.56 no.2 pp. 354-358
ISSN:
1938-4351
Subject:
Salmonella Enteritidis; bacterial antigens; blood serum; chickens; flagellin; flagellum; immunoglobulin A; immunoglobulin G; interferon-gamma; liver; plate count; protective effect; recombinant antigens; secretion; vaccination; vaccines
Abstract:
... FliC, the flagellin antigen of Salmonella Enteritidis, was tested as a vaccine candidate for protective effect against a homologous challenge in chickens. After immunization with recombinant FliC (rFliC) or administration of phosphate-buffered saline (PBS) at 56 days old, the chickens were challenged with 10⁹ colony-forming units of Salmonella Enteritidis at 76 days old. The vaccinated birds showe ...
DOI:
10.1637/9986-111011-Reg.1