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... A glycopeptide elicitor prepared from germ tubes of the rust fungus Puccinia graminis Pers. f. sp. tritici Erikss. & Henn (Pgt),as well as chitin oligosaccharides, chitosan, and methyl jasmonate (MJ) stimulated lipoxygenase (LOX) activity (E.C. 1.13.11.12) in wheat (Triticum aestivum) leaves. Immunoblot analysis using anti-LOX antibodies revealed the induction of 92- and 103-kD LOX species after P ...
... When a series of chitin oligosaccharides was added into a rice suspension culture, N-acetylchito- hexaose, N-acetylchitopentaose, and N-acetylchitotetraose caused an increase in extracellular chitinase activity, mainly due to induction of a class III chitinase. In the case of N-acetylchitohexaose, a substantial increase in the chitinase activity was observed at a concentration higher than 0.01 μg/ ...
... N-acetylchitooligosaccharides (fragments of chitin) elicit defense responses, including phytoalexin production, in suspension-cultured rice cells. They induced rapid and transient membrane depolarization accompanied by a transient increase in net Cl- -efflux. The membrane depolarization was not affected by anaerobiosis or azide, suggesting that the major part of the depolarization was mediated by ...
... Chitin synthase (EC 2.4.1.16) is a crucial enzyme responsible for chitin biosynthesis in all chitin-containing organisms. This paper reports a complete cDNA encoding chitin synthase 1 (AqCHS1), change of AqCHS1 mRNA level in response to diflubenzuron exposure, and concentration-dependent effect of diflubenzuron on chitin synthesis in the common malaria mosquito (Anopheles quadrimaculatus). The cDN ...
eclosion; Anopheles quadrimaculatus; insect growth; chemical composition; diflubenzuron; pupae; dose response; body weight; nikkomycins; mosquito control; toxicity; mortality; chitin; larvae
Abstract:
... Toxicities of three chitin synthesis inhibitors (diflubenzuron, nikkomycin Z and polyoxin D) were evaluated using second instars of the common malaria mosquito, Anopheles quadrimaculatus Say (Diptera: Culicidae). Neither nikkomycin Z nor polyoxin D at 50 μg/liter caused significant larval mortality, although they reduced the body weight of the survivors by 20.5 and 33.8%, respectively, in 48 h. In ...
... BACKGROUND: Previous studies showed that mammalian galectin-1 (GAL1) could interact with chitosan or chitin, one component of the peritrophic membrane (PM). This finding suggests that the PM could be a target of GAL1, which prompted the authors to explore the effect of GAL1 on larval growth and its potential mechanism.RESULTS: The development of Plutella xylostella (L.) larvae was significantly di ...
... Lectins have demonstrated significant levels of protection against different pests when expressed in transgenic plants. The effects of the coagulant Moringa oleifera lectin (cMoL) on moth flour (Anagasta kuehniella) were tested by incorporating the protein in an artificial diet at levels of 0.5%, 1% and 2% (w/w), respectively. cMoL showed a dose-dependent effect on average larval weight and a seri ...
... The effects of sublethal concentrations 0.1, 0.5, and 1.2 micrograms mL-1 of the chitin synthesis inhibitor, hexaflumuron, on larval growth and development, the count and proportion of hemocytes, and carbohydrate content (trehalose and glyceride) in hemolymph were investigated in the cutworm, Spodoptera litura (Fabricious) (Lepidoptera: Noctuidae). When 3rd instar larvae were subjected to the subl ...
... Chitin has been chemically extracted from the Egyptian shrimp shell waste. The obtained chitin was transformed into the more soluble chitosan. Chitin and chitosan were characterized using Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectrometry. These products were also characterized by their nematicidal potential against the root-knot nematode, Meloidogyne incogn ...
Mariana C.C. Silva; Lucimeire A. Santana; Reinhard Mentele; Rodrigo S. Ferreira; Antônio de Miranda; Rosemeire A. Silva-Lucca; Misako U. Sampaio; Maria T.S. Correia; Maria L.V. Oliva
... A new lectin, BfL, was purified from Bauhinia forficata seeds by ammonium sulfate fractionation, DEAE-Sephadex ion exchange chromatography, Sepharose-4B and chitin affinity chromatographies and Superdex 75 size exclusion chromatography. The molecular homogeneity and purity of BfL were assessed by reversed-phase HPLC. BfL appeared as a single band of approximately 27.0kDa on SDS-PAGE under non-redu ...
... A novel chitin derivative with an alternating N-l-cysteinyl group was successfully prepared. The precursor chitin derivative with an alternating N-l-thioprolinyl group could be obtained via chitinase-catalyzed polymerization of the N′-l-thioprolinyl chitobiose oxazoline derivative as a transition state analog substrate monomer under weak alkaline conditions. Conversion of the l-thioprolinyl group ...
Bartel Vanholme; Ruben Vanholme; Halbay Turumtay; Geert Goeminne; Igor Cesarino; Florence Goubet; Kris Morreel; Jorge Rencoret; Vincent Bulone; Cortwa Hooijmaijers; Riet De Rycke; Godelieve Gheysen; John Ralph; Marc De Block; Frank Meulewaeter; Wout Boerjan
... Crab shells waste were fermented using six protease-producing Bacillus species (Bacillus subtilis A26, Bacillus mojavensis A21, Bacillus pumilus A1, Bacillus amyloliquefaciens An6, Bacillus licheniformis NH1 and Bacillus cereus BG1) for the production of chitin and fermented-crab supernatants (FCSs). In medium containing only crab shells, the highest demineralization DM was obtained with B. lichen ...
... Immunostimulants increase the immunocompetency and disease resistance of fish by enhancing immune responses. This study determined the in vitro and dietary effects of chitin on immune responses of catla, Catla catla. Catla head‐kidney leukocytes were cultured in complete RPMI‐1640 medium containing 0.01, 0.1, and 1.0 mg/mL of chitin. Leucocyte viability and different cellular immune responses, nam ...
... Thecosome pteropods, a group of calcifying holoplanktonic mollusks, have recently become a research focus due to their potential sensitivity to increased levels of anthropogenic dissolved CO2 in seawater and the accompanying ocean acidification. Some populations, however, already experience high CO2 in their natural distribution during diel vertical migrations. To achieve a better understanding of ...
... Chitin was recovered through enzymatic deproteinization of the Norway lobster (Nephrops norvegicus) processing by-products. The obtained chitin was characterized and converted into chitosan by N-deacetylation, the acid-soluble form of chitin. Chitosan samples were then characterized by Fourier transform infrared spectroscopy (FTIR) and 13 Cross polarization magic angle spinning nuclear magnetic re ...
... The volatile oil (1.12%) from the Z. zerumbet rhizomes were extracted using Clevenger’s distillation. The GC and GC-MS profile of the Z. zerumbet volatiles revealed several components of which Zerumbone was the principal compound (49.8%) followed by α-Caryophyllene (20.1%) and trace of other compounds. The antifungal property of volatiles was studied against Aspergillus flavus and Aspergillus ochr ...
... To accomplish consistent, long-term, integrated management (IPM) of the Colorado potato beetle, Leptinotarsa decemlineata (Say), research assessing the potential of novel, IPM-compatible insecticides is essential. Novaluron is a potent benzoylurea insecticide. In the present paper, we found that novaluron ingestion by the fourth-instar larvae inhibited foliage consumption, reduced larval fresh wei ...