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... Plant phospholipase D (PLD) and its product phosphatidic acid (PA) function in both abiotic and biotic stress signaling. However, to date, a PLD gene conferring the desired resistance to both biotic and abiotic stresses has not been found in cotton. Here, we isolated and identified a PLD gene GhPLDδ from cotton (Gossypium hirsutum), which functions in Verticillium wilt resistance and salt toleranc ...
... Acidic mammalian chitinase (AMCase) could hydrolyze chitin into (GlcNAc)₂ at pH 2.0. Therefore, it was essential to develop an engineered strain with a high-yield AMCase. Herein, multiple strategies including codon optimization, multiple expression cassettes insertion, co-expression of helper proteins, were developed to construct a recombinant Pichia pastoris with the high-level secretion of AMCas ...
biodegradability; byproducts; chitin; composts; expert opinion; plant protection; research and development; seedlings; soil; soil quality; wastes
Abstract:
... Insects have tremendous potential for utilising the waste generated from other agricultural sectors to produce high value protein. The by-product of insect production, insect waste streams, can be recycled and repurposed for promoting the health of crops and soil as the chitin, present in the moulted skins, acts as a substrate for beneficial microbes that aid in plant protection. However, because ...
... Chitin is an excellent material for the synthesis of nanoparticles because it is an elicitor and can form nanostructured materials. The application of chitin nanoparticles (CNPs) in plants can activate early defense responses associated with chitin. In this study, CNPs were synthesized by water in oil (W/O) emulsion using an aqueous chitin solution. The CNPs were characterized and used to evaluate ...
Arabidopsis; Western blotting; binding properties; cell membranes; chitin; cytoplasm; genes; germination; global warming; heat stress; heat tolerance; jasmonic acid; plant growth; plant hormones; seedlings; signal transduction; temperature; transgenic plants
Abstract:
... Due to global warming, high temperature has become the main abiotic stress affecting plant growth worldwide. LysM-containing receptor-like kinase 4 (LYK4) is the receptor for chitin, and ERECTA(ER) is a key factor in plant tolerance to high temperature. In this study, we constructed a chitin-induced chimeric LYK4-ER gene, in which the extracellular region and transmembrane domain of the LYK4 gene ...
... Plant resistance inducers (PRIs) are compounds that protect plants from diseases by activating immunity responses. Exogenous treatment with glutamate (Glu), an important amino acid for all living organisms, induces resistance against fungal pathogens in rice and tomato. To understand the molecular mechanisms of Glu-induced immunity, we used the Arabidopsis model system. We found that exogenous tre ...
... The biopolymer chitosan is a derivative of chitin, which can promote plant growth and protect plants from phytopathogens. This study aimed to evaluate the efficacy of chitosan as a biostimulant and a biorational agent to protect cucumber plants from damping-off disease caused by Phytophthora capsici. Cucumber seeds were treated with a range of chitosan concentrations, viz. 0, 125, 250, and 500 ppm ...
Priscila de Andrade Gonçalves; Manoelito Coelho dos Santos Junior; Catiane do Sacramento Sousa; Aristóteles Góes-Neto; Edna Dora Martins Newman Luz; Virgínia Oliveira Damaceno; Ana Rosa Rocha Niella; José Maria Barbosa Filho; Sandra Aparecida de Assis
... The inhibition of the fungus Moniliophthora perniciosa, which is the causal agent of witches’ broom disease in Theobroma cacao L., is the main focus of the present study. The use of phytoalexins has provided disease control that is as efficient as control with the use of fungicides, with the advantage of not harming humans and the environment. In this sense, the objective was to evaluate the actio ...
... Microbial antagonists and their bioactive metabolites provide one of the best alternatives to chemical pesticides to control crop disease for sustainable agriculture and global food security.The rice endophyte Streptomyces hygroscopicus OsiSh-2, with remarkable antagonistic activity towards the rice blast fungus Magnaporthe oryzae, was reported in our previous study. The present study deciphered t ...
... The plant primary energy metabolism is profoundly reorganized under biotic stress conditions and there is increasing evidence for a role of the fermentative pathway in biotic interactions. Previously we showed via transient gene silencing or overexpression a function of barley alcohol dehydrogenase 1 (HvADH-1) in the interaction of barley with the parasitic fungus Blumeria graminis f.sp. hordei (B ...
... Plant sense potential microbial pathogen using pattern recognition receptors (PRRs) to recognize pathogen‐associated molecular patterns (PAMPs). The Lectin receptor‐like kinase genes (LecRKs) are involved in various cellular processes mediated by signal transduction pathways. In the present study, an L‐type lectin receptor kinase gene LecRK‐V was cloned from Haynaldia villosa, a diploid wheat rela ...
... Chitin, a linear polymer of N-acetyl-d-glucosamine, and chitosans, fully or partially deacetylated derivatives of chitin, are known to elicit defense reactions in higher plants. We compared the ability of chitin and chitosan oligomers and polymers (chitin oligomers with degree of polymerization [DP] 3 to 8; chitosan oligomers with degree of acetylation [DA] 0 to 35% and DP 3 to 15; chitosan polyme ...
... Spent mushroom substrate is considered as one of the ingredients of growing media which could be used partially as peat substitution in the near future. The aim of this research was to enhance the plant biostimulant activity of a spent Pleurotus substrate (SPS) coming from mushroom cultivation on wheat straw, and containing 39.5±1.3% cellulose, 14.7±0.5% hemicellulose, 19.6±1.1% lignin, 7.8±0,3% c ...
... Molecular interaction between the causal agent of blackleg disease, Leptosphaeria maculans (Lm), and its host, Brassica napus, is largely unknown. We applied a deep RNA‐sequencing approach to gain insight into the pathogenicity mechanisms of Lm and the defence response of B. napus. RNA from the infected susceptible B. napus cultivar Topas DH16516, sampled at 2‐day intervals (0–8 days), was sequenc ...
... Chitosan, a partially deacetylated form of the natural and biodegradable biopolymer chitin, has been used as plant growth promoter in agriculture. The aim of this work was to investigate the growth promoting responses induced by chitosan at the physiological and molecular level in rice (Oryza sativa L.) seedlings. The combination of the degree of deacetylation (DD), molecular weight and concentrat ...
... In agricultural environments where canola (Brassica napus) is grown, slow germination can increase the susceptibility of seedlings to pathogens, delay maturity, and decrease yield. Bacterial products that enhance germination have been identified for a variety of plants. Three signal molecules were investigated: Bradyrhizobium japonicum 532C product lipo-chitooligosaccharide (LCO), Bacillus thuring ...
... Heterobasidion annosum is widely known as a major root and butt rot pathogen of conifer trees, but little information is available on its interaction with the roots of herbaceous angiosperm plants. We investigated the infection biology of H. annosum during challenge with the angiosperm model Arabidopsis and monitored the host response after exposure to different hormone elicitors, chemicals (chiti ...
... Post-translational modification by ubiquitin plays a key role in the regulation of endocytic degradation in which ubiquitinated plasma membrane cargos are transported to the vacuole for degradation dependent on the ESCRT (endosomal sorting complex required for transport) machinery. Arabidopsis AMSH3 (ASSOCIATED MOLECULE WITH THE SH3 DOMAIN OF STAM 3) is a deubiquitinating enzyme that interacts wit ...
... Enhancement of disease suppressive properties of soils will limit disease development, thus, being of great importance for sustainable agricultural farming systems. The current research demonstrated that suppressiveness against Rhizoctonia solani AG 2-2IIIB in sugar beet could be elevated in different soils. Feather meal was most effective and disease spread was reduced with 36-87% in clay as well ...
... A total of eight actinomycetes were isolated from Glomus mosseae spores predominant in the rhizosphere of guava (Psidium guajava L. cv Arka Mridula) orchards in Karnataka State, southern India. Five isolates were identified using RNA polymerase β-subunit gene primers as Streptomyces fradiae, S. avermitilis, S. cinnamonensis, Leifsonia poae and Streptomyces canus. All the isolates were tested for b ...
... Digitalis purpurea L. (Scrophulariaceae; Foxglove) is a source of cardiotonic glycosides such as digitoxin and digoxin which are commercially applied in the treatment to strengthen cardiac diffusion and to regulate heart rhythm. This investigation deals with in vitro propagation and elicited production of cardiotonic glycosides digitoxin and digoxin in shoot cultures of D. purpurea L. In vitro ger ...
... Noni, Morinda citrfolia L., is a miracle plant which has more than 150 nutraceuticals and is extensively cultivated in the coastal areas of India. While its cultivation and medical significance is gaining popularity, it is important to understand various stress factors that the crop encounters. Root-knot nematode, Meloidogyne incognita is one of the most damaging pathogens infesting the crop at th ...
... AtPeps are a family of small peptides in Arabidopsis that are believed to act as endogenous amplifiers of the plant’s innate immune response. In our recent study,¹⁰ we showed that in Arabidopsis leaf disks, bacterial MAMPs (microbe-associated molecular patterns) such as the flagellin derived elicitor flg22, greatly enhanced the release of reactive oxygen species (ROS) triggered by a subsequent AtP ...
... Plant phenotypes resistant and susceptible to fungal pathogens are usually scored using qualitative, subjective methods that are based upon disease symptoms or by an estimation of the amount of visible fungal growth. Given that plant resistance genes often confer partial resistance to fungal pathogens, a simple, sensitive, nonsubjective quantitative method for measuring pathogen growth would be hi ...
... Chitosan is a deacethylated derivative of chitin derived from the crustacean group. Thus it can be degraded in nature and is safe for living organisms. It has been shown to promote the growth of various plants. For example, chitosan has been reported to increase the growth rate and early flowering in orchids. The objective of this experiment was to study the effect of chitosan on in vitro growth o ...
... The populations of Acacia koa (koa), a tree legume native to Hawaii, are currently at risk due to infection by the wilt-inducing fungal pathogen, Fusarium oxysporum. To identify koa families that are resistant to F. oxysporum, it is necessary to develop a screening method through use of a biomarker gene. The purpose of this study was to develop a molecular method for detecting resistance through t ...
... Application of crab shell chitin or pentamer chitin oligosaccharide to Arabidopsis seedlings increased tolerance to salinity in wild-type but not in knockout mutants of the LysM Receptor-Like Kinase1 (CERK1 / LysM RLK1) gene, known to play a critical role in signaling defense responses induced by exogenous chitin. Arabidopsis plants overexpressing the endochitinase chit36 and hexoaminidase excy1 g ...
... Despite its proven agronomic value, the plant disease suppressive effect of composts from olive waste has not been adequately investigated. In the present study, the disease suppressive potential of two olive waste (OW) composts against soil-borne plant pathogens was investigated. Both OW composts showed sizeable, active microbial populations, which were able to grow actively on chitin and cellulo ...
extracellular space; secretion; heavy metals; seedlings; plant response; mass spectrometry; risk; two-dimensional gel electrophoresis; barley; Hordeum vulgare; cadmium; washing; leaves; apoplast; aerial parts; proteomics; chitin; protein synthesis; allergenicity; chitinase
Abstract:
... The effect of cadmium on protein expression in the aerial parts of barley (Hordeum vulgare L. cv. ‘Mandolina’) seedlings was investigated by proteomic analysis of leaf apoplast proteins. Dramatic changes were observed in the protein pattern of intercellular washing fluid from Cd-treated (0–300 μM) barley leaves both by 1D- and 2D-PAGE. By mass spectrometric (MALDI-TOF and/or LC-MS/MS) analy ...
... Sixty-three chitinase producing rhizobacteria (CRB) were isolated from 57 rhizospheric soil samples of tomato (Lycopersicon esculentum Mill.) growing in different regions of Karnataka, India. Among these, 13 CRB isolates were selected based on their ability to produce chitinase, colonize roots of tomato seedlings and reduce Fusarium wilt incidence. Four of these isolates produced statistically hig ...
Giovanni Povero; Elena Loreti; Chiara Pucciariello; Antonietta Santaniello; Donata Di Tommaso; Gianluca Di Tommaso; Dimos Kapetis; Francesca Zolezzi; Alberto Piaggesi; Pierdomenico Perata
... In nature, plants can recognize potential pathogens, thus activating intricate networks of defense signals and reactions. Inducible defense is often mediated by the detection of microbe or pathogen associated molecular pattern elicitors, such as flagellin and chitin. Chitosan, the deacetylated form of chitin, plays a role in inducing protection against pathogens in many plant species. We evaluated ...
... Rhizobacteria isolated from cotton roots exhibiting antagonism towards seedling blight and leaf blight of cotton were tested for their efficiency against the insect pest American bollworm (Helicoverpa armigera). The bioformulation developed using Bacillus subtilis (isolate DGL9) + chitin was found to be detrimental to the developmental stages of H. armigera (larva, pupa and adult) by causing larva ...
... Jatropha (Jatropha curcas L.) is a non-edible oil-seed plant with adaptability to marginal semi-arid lands and wastelands. The Indian Government is promoting jatropha to reduce dependence on the crude oil and to achieve energy independence by the year 2012, under the National Biodiesel Mission. Selected strains of Bacillus spp., either supplemented with or without chitin, were tested for their abi ...
... A plant growth-promoting Paenibacillus lentimorbus NRRL B-30488 (B-30488) was isolated from cows' milk. Bacterial colonization and growth responses of different plant species after inoculation with B-30488 were evaluated in a controlled environment and in microplot assays. Survival and colonization of B-30488 in the phytosphere of plants and soil was monitored using a chromosomally located rifampi ...
... Chitin is a major component of fungal walls and insect exoskeletons. Plants produce chitinases upon pathogen attack and chito-oligomers induce defense responses in plants, though the exact mechanism behind this response is unknown. Using the ATH1 Affymetrix microarrays consisting of about 23,000 genes, we examined the response of Arabidopsis (Arabidopsis thaliana) seedlings to chito-octamers and h ...
... The effect of Trichoderma atroviride, T. harzianum, T. longibrachiatum, Clonostachys rosea and Bacillus subtilis isolates applied to wheat seeds against Rhizoctonia cerealis disease of seedlings was investigated under controlled greenhouse conditions. Most Trichoderma isolates significantly reduced the incidence of disease compared with the infected control. Bacillus subtilis was also effective ag ...
... A glycopeptide elicitor prepared from germ tubes of the rust fungus Puccinia graminis Pers. f. sp. tritici Erikss. & Henn (Pgt),as well as chitin oligosaccharides, chitosan, and methyl jasmonate (MJ) stimulated lipoxygenase (LOX) activity (E.C. 1.13.11.12) in wheat (Triticum aestivum) leaves. Immunoblot analysis using anti-LOX antibodies revealed the induction of 92- and 103-kD LOX species after P ...
... Astract: Buprofezin (2-tert-butylimino-3-isopropyl-5-phenyl, 3,4,5,6,tetrahydro-2-thiadiazine-4-one) sprayed on cotton seedlings under greenhouse conditions suppressed embryogenesis and progeny formation of the sweetpotato whitefly (SPWF), Bemisia tabaci (Gennadius). The estimated concentration for 50% inhibition of egg hatch applied through adults was ca. 15 mg/liter and that for 50% cumulative l ...