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1. Acetaminophen Disrupts the Development of Pharyngeal Arch-Derived Cartilage and Muscle in Zebrafish

Author:
Derrick M. Glasco; Zhidong Wang; Seonwoo Kang; Avery T. Funkhouser
Source:
Journal of developmental biology 2022 v.10 no.3 pp. -
ISSN:
2221-3759
Subject:
Danio rerio; acetaminophen; apoptosis; cartilage; chondrogenesis; collagen; embryogenesis; lethal dose; liver; muscles; neural crest; pharynx
Abstract:
... Acetaminophen is a common analgesic, but its potential effects on early embryonic development are not well understood. Previous studies using zebrafish (Danio rerio) have described the effects of acetaminophen on liver development and physiology, and a few have described gross physiological and morphological defects. Using a high but non-embryonic lethal dose of acetaminophen, we probed for defect ...
DOI:
10.3390/jdb10030030

2. Biomaterial-mediated presentation of wnt5a mimetic ligands enhances chondrogenesis and metabolism of stem cells by activating non-canonical Wnt signaling

Author:
Yingrui Deng; Xiaoting Zhang; Rui Li; Zhuo Li; Boguang Yang; Peng Shi; Honglu Zhang; Chunming Wang; Chunyi Wen; Gang Li; Liming Bian
Source:
Biomaterials 2022 v.281 pp. 121316
ISSN:
0142-9612
Subject:
biocompatible materials; biomarkers; biosynthesis; cartilage; chondrogenesis; encapsulation; gene expression; glucose; humans; hyaluronic acid; hydrogels; hypertrophy; ligands; medicine; mitochondria; musculoskeletal system; peptides; tissue repair
Abstract:
... The presentation of development-relevant bioactive cues by biomaterial scaffolds is essential to the guided differentiation of seeded human mesenchymal stem cells (hMSCs) and subsequent tissue regeneration. Wnt5a is a critical non-canonical Wnt signaling ligand and plays a key role in the development of musculoskeletal tissues including cartilage. Herein we investigate the efficacy of biofunctiona ...
DOI:
10.1016/j.biomaterials.2021.121316

3. Chsy1 deficiency reduces extracellular matrix productions and aggravates cartilage injury in osteoarthritis

Author:
Zhaojie Lyu; Yifeng Da; Hongkai Liu; Zhihao Wang; Yong Zhu; Jing Tian
Source:
Gene 2022 v.827 pp. 146466
ISSN:
0378-1119
Subject:
biomarkers; bone formation; cartilage; cell lines; chondrocytes; chondrogenesis; chondroitin sulfate; extracellular matrix; genes; glycosyltransferases; hypertrophy; mice; osteoarthritis
Abstract:
... Osteoarthritis (OA) is a kind of degenerative joint disease marked by the destruction of articular cartilage due to the degeneration of chondrocytes. CHSY1, one of the glycosyltransferases, is involved in the synthesis of chondroitin sulfate. Herein, we found that the expression of Chsy1 was decreased in the knee cartilage of OA rats. In order to investigate the role of CHSY1 in chondrogenesis and ...
DOI:
10.1016/j.gene.2022.146466

4. Genetic basis investigation of wattle phenotype in goat using genome‐wide sequence data

Author:
Wei‐Yi Zhang; Ying Yuan; Hao‐Yuan Zhang; Yong‐Meng He; Cheng‐Li Liu; Lu Xu; Bai‐Gao Yang; Hang‐Xing Ren; Gao‐Fu Wang; Guang‐Xin E
Source:
Animal genetics 2022 v.53 no.5 pp. 700-705
ISSN:
0268-9146
Subject:
animal genetics; cartilage; chondrogenesis; chromosomes; ears; gene ontology; genome; genome-wide association study; goats; neck; phenotype
Abstract:
... In domestic goats, wattles often appear in even numbers, mostly on the neck and a few under the ear. Goat wattle is composed of ectopic cartilage tissue covered by skin and was reported as a dominant inheritance. Thirty‐eight goats from two Southwest Chinese breeds were studied to elucidate the genetic basis of wattle phenotype in goat. Their genomes were sequenced for wide‐genome selective sweep ...
DOI:
10.1111/age.13235

5. Geometric analysis of chondrogenic self-organisation of embryonic limb bud cells in micromass culture

Author:
Rebecca A. Rolfe; Claire A. Shea; Paula Murphy
Source:
Cell and tissue research 2022 v.388 no.1 pp. 49-62
ISSN:
0302-766X
Subject:
analytical methods; antagonists; bone formation; cartilage; chondrogenesis; gene expression; geometry; hypertrophy; ligands; models; research; vertebrates; wnt proteins
Abstract:
... Spatial and temporal control of chondrogenesis generates precise, species-specific patterns of skeletal structures in the developing vertebrate limb. The pattern-template is laid down when mesenchymal cells at the core of the early limb bud condense and undergo chondrogenic differentiation. Although the mechanisms involved in organising such complex patterns are not fully understood, the interplay ...
DOI:
10.1007/s00441-021-03564-y

6. Induction of chondrogenesis with a RANKL-binding peptide, WP9QY, in vitro and in vivo in a rabbit model

Author:
Yuriko Furuya; Hisashi Mera; Maki Itokazu; Shozaburo Terai; Hiroaki Nakamura; Shigeyuki Wakitani; Hisataka Yasuda
Source:
Biochemical and biophysical research communications 2022 v.602 pp. 98-104
ISSN:
0006-291X
Subject:
bone formation; cartilage; chondrocytes; chondrogenesis; drugs; humans; ligands; osteoarthritis; osteoblasts; osteoclasts; peptides; rabbits; research
Abstract:
... WP9QY (W9) is a receptor activator of nuclear factor-κB ligand (RANKL)-binding peptide that inhibits osteoclastogenesis by blunting the RANKL-RANK interaction, and also increases osteoblastogenesis via RANKL reverse signaling. W9 has dual effects on osteoclasts and osteoblasts; however, it is unknown whether the peptide has an effect on chondrocytes. Here, we report that W9 induces proliferation a ...
DOI:
10.1016/j.bbrc.2022.03.019

7. Maternal effect and dietary supplementation of estradiol-17β on female zebrafish (Danio rerio) affects the swimming behavior and stress-coping styles of its offspring

Author:
Su Mei Wu; Yu-Jen Tseng; Te-Hao Chen
Source:
Comparative biochemistry and physiology 2022 v.252 pp. 109211
ISSN:
1532-0456
Subject:
Danio rerio; cartilage; chondrogenesis; cold stress; cortisol; dietary supplements; fecundity; females; fish; juveniles; larvae; males; maternal effect; progeny
Abstract:
... Extrinsic estradiol-17β (E2) is an environmental hormone. Female fish exposure to waterborne E2 might affect the development of craniofacial cartilage of its offspring. The present study investigates the effects of maternal E2 on larval craniofacial cartilage development by administering oral feed containing E2 (F-E2) to female zebrafish, and examines whether the swimming behavior and their stress ...
DOI:
10.1016/j.cbpc.2021.109211

8. Mutation of foxl1 Results in Reduced Cartilage Markers in a Zebrafish Model of Otosclerosis

Author:
Alexia Hawkey-Noble; Justin A. Pater; Roshni Kollipara; Meriel Fitzgerald; Alexandre S. Maekawa; Christopher S. Kovacs; Terry-Lynn Young; Curtis R. French
Source:
Genes 2022 v.13 no.7 pp. -
ISSN:
2073-4425
Subject:
Danio rerio; bone density; bone formation; calcification; cartilage; chondrogenesis; collagen; genes; mammals; meta-analysis; middle ear; mineralization; models; mutants; mutation; osteoporosis; skeletal development; skeleton; transcription factors
Abstract:
... Bone diseases such as otosclerosis (conductive hearing loss) and osteoporosis (low bone mineral density) can result from the abnormal expression of genes that regulate cartilage and bone development. The forkhead box transcription factor FOXL1 has been identified as the causative gene in a family with autosomal dominant otosclerosis and has been reported as a candidate gene in GWAS meta-analyses f ...
DOI:
10.3390/genes13071107

9. Nitrogen-doped titanium dioxide films fabricated via magnetron sputtering for vascular stent biocompatibility improvement

Author:
Zhilei Sun; Igor A. Khlusov; Kirill E. Evdokimov; Maksim E. Konishchev; Oleg S. Kuzmin; Olga G. Khaziakhmatova; Vladimir V. Malashchenko; Larisa S. Litvinova; Sven Rutkowski; Johannes Frueh; Anna I. Kozelskaya; Sergei I. Tverdokhlebov
Source:
Journal of colloid and interface science 2022 v.626 pp. 101-112
ISSN:
0021-9797
Subject:
adipose tissue; biocompatibility; cell adhesion; cell proliferation; chondrogenesis; energy; films (materials); glass; humans; nitrogen; oxygen; titanium dioxide; wettability
Abstract:
... Nowadays, vascular stents are commonly used to treat cardiovascular diseases. This article focuses on the influence of nitrogen doping of titanium dioxide thin films, utilized for coating metallic stents to improve their biological properties and biocompatibility. The hereby-investigated titanium oxide thin films are fabricated by magnetron sputtering in a reactive gas atmosphere consisting of arg ...
DOI:
10.1016/j.jcis.2022.06.114

10. The Role of Protein Kinase CK2 in Development and Disease Progression: A Critical Review

Author:
Daniel Halloran; Venu Pandit; Anja Nohe
Source:
Journal of developmental biology 2022 v.10 no.3 pp. -
ISSN:
2221-3759
Subject:
adipogenesis; adulthood; bone formation; chondrogenesis; disease progression; embryonic mortality; evolution; immune response; neurogenesis; neurons; protein kinases; spermatogenesis; therapeutics; viability
Abstract:
... Protein kinase CK2 (CK2) is a ubiquitous holoenzyme involved in a wide array of developmental processes. The involvement of CK2 in events such as neurogenesis, cardiogenesis, skeletogenesis, and spermatogenesis is essential for the viability of almost all organisms, and its role has been conserved throughout evolution. Further into adulthood, CK2 continues to function as a key regulator of pathway ...
DOI:
10.3390/jdb10030031

11. Supplementation of articular cartilage-derived chondroprogenitors with bone morphogenic protein-9 enhances chondrogenesis without affecting hypertrophy

Author:
Kawin Padmaja; Soosai Manickam Amirtham; Grace Rebekah; Solomon Sathishkumar; Elizabeth Vinod
Source:
Biotechnology letters 2022 v.44 no.9 pp. 1037-1049
ISSN:
0141-5492
Subject:
adhesion; biotechnology; cell differentiation; chondrocytes; chondrogenesis; culture media; fibronectins; gene expression; glycosaminoglycans; growth models; humans; hypertrophy; osteoarthritis; parathyroid hormone; prostheses; qualitative analysis; therapeutics; toluidine blue
Abstract:
... INTRODUCTION: Chondroprogenitors (CPCs) have emerged as a promising cellular therapy for cartilage-related pathologies due to their inherent primed chondrogenic potential. Studies report that the addition of growth factors such as parathyroid hormone (PTH) and Bone Morphogenic Protein (BMP) enhance the chondroinducive potential in chondrocytes and mesenchymal stem cells. This study evaluated if su ...
DOI:
10.1007/s10529-022-03280-9

12. Temporospatial regulation of intraflagellar transport is required for the endochondral ossification in mice

Author:
Hiroyuki Yamaguchi; Megumi Kitami; Karin H. Uchima Koecklin; Li He; Jianbo Wang; William R. Lagor; Daniel S. Perrien; Yoshihiro Komatsu
Source:
Developmental biology 2022 v.482 pp. 91-100
ISSN:
0012-1606
Subject:
Erinaceidae; bone formation; chondrocytes; chondrogenesis; mutants
Abstract:
... Ciliogenic components, such as the family of intraflagellar transport (IFT) proteins, are recognized to play key roles in endochondral ossification, a critical process to form most bones. However, the unique functions and roles of each IFT during endochondral ossification remain unclear. Here, we show that IFT20 is required for endochondral ossification in mice. Utilizing osteo-chondrocyte lineage ...
DOI:
10.1016/j.ydbio.2021.12.004

13. A bioprintable gellan gum/lignin hydrogel: a smart and sustainable route for cartilage regeneration

Author:
Maria A. Bonifacio; Stefania Cometa; Andrea Cochis; Annachiara Scalzone; Piergiorgio Gentile; Alessandro C. Scalia; Lia Rimondini; Piero Mastrorilli; Elvira De Giglio
Source:
International journal of biological macromolecules 2022 v.216 pp. 336-346
ISSN:
0141-8130
Subject:
Staphylococcus epidermidis; antibacterial properties; ascorbic acid; cartilage; cell growth; chondrogenesis; collagen; crosslinking; gellan gum; hydrogels; lignin; magnesium
Abstract:
... In this work a hydrogel, based on a blend of two gellan gums with different acyl content embedding lignin (up to 0.4%w/v) and crosslinked with magnesium ions, was developed for cartilage regeneration. The physico-chemical characterizations established that no chemical interaction between lignin and polysaccharides was detected. Lignin achieved up to 80 % of ascorbic acid's radical scavenging activ ...
DOI:
10.1016/j.ijbiomac.2022.07.002

14. A sonication-induced silk-collagen hydrogel for functional cartilage regeneration

Author:
Shihe Long; Danyang Huang; Zihan Ma; Shuaiguang Shi; Yun Xiao; Xingdong Zhang
Source:
Journal of materials chemistry B 2022 v.10 no.26 pp. 5045-5057
ISSN:
2050-7518
Subject:
cartilage; cell culture; chondrogenesis; collagen; fibroins; gene expression; glycosaminoglycans; hydrogels; rabbits; shrinkage; sonication
Abstract:
... Cartilage tissue has limited self-regeneration capacity and current treatment methods often result in fibrocartilage formation. Although collagen has shown the ability to induce chondrogenesis of mesenchymal stem cells (MSCs) and regenerate hyaline cartilage, the application of a pure collagen hydrogel is inherently limited by its fast degradation, poor mechanical properties and excessive cell-med ...
DOI:
10.1039/d2tb00564f

15. 3D-Bioprinted Difunctional Scaffold for In Situ Cartilage Regeneration Based on Aptamer-Directed Cell Recruitment and Growth Factor-Enhanced Cell Chondrogenesis

Author:
Zhen Yang; Tianyuan Zhao; Cangjian Gao; Fuyang Cao; Hao Li; Zhiyao Liao; Liwei Fu; Pinxue Li; Wei Chen; Zhiqiang Sun; Shuangpeng Jiang; Zhuang Tian; Guangzhao Tian; Kangkang Zha; Tingting Pan; Xu Li; Xiang Sui; Zhiguo Yuan; Shuyun Liu; Quanyi Guo
Source:
ACS applied materials & interfaces 2021 v.13 no.20 pp. 23369-23383
ISSN:
1944-8252
Subject:
biodegradability; bioprinting; cartilage; cell adhesion; chondrogenesis; extracellular matrix; gelatin; oligonucleotides; polymers; rabbits; strength (mechanics); therapeutics
Abstract:
... Articular cartilage (AC) lesions are fairly common but remain an obstacle for clinicians and researchers due to their poor self-healing capacity. Recently, a promising therapy based on the recruitment of autologous mesenchymal stem cells (MSCs) has been developed for the regeneration of full-thickness cartilage defects in the knee joint. In this study, a 3D-bioprinted difunctional scaffold was dev ...
DOI:
10.1021/acsami.1c01844

16. 3D-Printed Porous Scaffolds of Hydrogels Modified with TGF-β1 Binding Peptides to Promote In Vivo Cartilage Regeneration and Animal Gait Restoration

Author:
Xiaoquan Ding; Jingming Gao; Xiaoye Yu; Jiayue Shi; Jun Chen; Lin Yu; Shiyi Chen; Jiandong Ding
Source:
ACS applied materials & interfaces 2022 v.14 no.14 pp. 15982-15995
ISSN:
1944-8252
Subject:
bone formation; cartilage; chemical bonding; chondrogenesis; gait; gelatin; hydrogels; hydroxyapatite; osteoarthritis; peptides; therapeutics; tissue repair
Abstract:
... The treatment of cartilage injury and osteoarthritis has been a classic problem for many years. The idea of in situ tissue regeneration paves a way for osteochondral repair in vivo. Herein, a hydrogel scaffold linked with bioactive peptides that can selectively adsorb transforming growth factor β1 (TGF-β1) was hypothesized to not only afford cell ingrowth space but also induce the endogenous TGF-β ...
DOI:
10.1021/acsami.2c00761

17. Altered microRNAs in C3H10T1/2 cells induced by p.E95K mutant IHH signaling

Author:
Wei Zhou; Luan Chen; Hao Wu; Ting Wang; Gang Ma; Baocheng Wang; Cong Wang; Na Zhang; Yingtian Zhang; Lin He; Shengying Qin; Xiaofang Sun; Hai Zhang; Lu Shen
Source:
Hereditas 2021 v.158 no.1 pp. 48
ISSN:
1601-5223
Subject:
Erinaceidae; cartilage; cell lines; chondrogenesis; gene expression; heterozygosity; microRNA; microarray technology; mutants; pathogenesis; reporter genes
Abstract:
... BACKGROUND: Indian Hedgehog (IHH), an important cell signaling protein, plays a key regulatory role in development of cartilage and chondrogenesis. Earlier studies have shown that heterozygous missense mutations in IHH gene may cause brachydactyly type A1 (BDA1), an autosomal dominant inheritance disease characterized by apparent shortness or absence of the middle phalanges of all digits. MicroRNA ...
DOI:
10.1186/s41065-021-00207-8

18. Anti-Inflammatory and Prochondrogenic In Situ-Formed Injectable Hydrogel Crosslinked by Strontium-Doped Bioglass for Cartilage Regeneration

Author:
Zhuochang Cai; Ying Li; Wei Song; Yaohua He; Haiyan Li; Xudong Liu
Source:
ACS applied materials & interfaces 2021 v.13 no.50 pp. 59772-59786
ISSN:
1944-8252
Subject:
biocompatible materials; bone marrow; cartilage; chondrogenesis; crosslinking; gene expression; glycosaminoglycans; humans; hydrogels; inflammation; macrophages; models; phenotype; secretion
Abstract:
... Directed differentiation of bone marrow mesenchymal stem cells (BMSCs) toward chondrogenesis plays a predominant role in cartilage repair. However, the uncontrolled inflammatory response to implants is found to impair the stability of scaffolds and the cartilage regeneration outcome. Herein, we fabricated an injectable hydrogel crosslinked by strontium-doped bioglass (SrBG) to modulate both human ...
DOI:
10.1021/acsami.1c20565

19. Assessment of a novel activity of Acacia nilotica leaf extract for chondrogenesis induction of Mesenchymal stem cells for dental applications

Author:
Yasir B. Fadhil; Mohammed Hadi Jaber; Hind M. Ahmed
Source:
Phytomedicine plus 2021 v.1 no.4 pp. 100087
ISSN:
2667-0313
Subject:
Acacia; Vachellia nilotica; cartilage; chondrocytes; chondrogenesis; collagen; ethanol; glycosaminoglycans; leaf extracts; orthopedics; protein synthesis; secondary metabolites; tooth pulp
Abstract:
... The Dearth of the self-rejuvenation capability of cartilage tissue is one of the frequent problems encountered by the dentist and orthopedic surgeons. Acacia nilotica commonly known as (babul, thorny acacia, Egyptian acacia or thorn mimosa) has wide range of medicinal uses. The current study was carried out to investigate the herbal-based chondrogenesis inducing ability of leaves extract of A. nil ...
DOI:
10.1016/j.phyplu.2021.100087

20. Bioinspired Injectable Hydrogels Dynamically Stiffen and Contract to Promote Mechanosensing-Mediated Chondrogenic Commitment of Stem Cells

Author:
Aman Mahajan; Akhilesh Singh; Dipak Datta; Dhirendra S. Katti
Source:
ACS applied materials & interfaces 2022 v.14 no.6 pp. 7531-7550
ISSN:
1944-8252
Subject:
Bombyx mori; carboxymethylcellulose; cartilage; chondrogenesis; fibroins; gelatin; hydrogels; mechanotransduction; silk
Abstract:
... Developing stiff and resilient injectable hydrogels that can mechanically support load-bearing joints while enabling chondrogenic differentiation of stem cells is a major challenge in the field of cartilage tissue engineering. In the present work, a triple-network injectable hydrogel system was engineered using Bombyx mori silk fibroin, carboxymethyl cellulose (CMC), and gelatin. The developed hyd ...
DOI:
10.1021/acsami.1c11840

21. Bioinspired polysaccharide hybrid hydrogel promoted recruitment and chondrogenic differentiation of bone marrow mesenchymal stem cells

Author:
Zhulian Li; Hongfu Cao; Yang Xu; Xing Li; XiaoWen Han; Yujiang Fan; Qing Jiang; Yong Sun; Xingdong Zhang
Source:
Carbohydrate polymers 2021 v.267 pp. 118224
ISSN:
0144-8617
Subject:
adhesion; adsorption; biomimetics; bone marrow; cartilage; catechol; chondrogenesis; collagen; dopamine; gene expression; hyaluronic acid; hydrogels; rabbits; secretion
Abstract:
... Cartilage regeneration by biomimetic cartilage matrix with synchronously recruited stem cells was one of ideal strategies. Inspired by catechol for proteins adhesion, dopamine modified polysaccharide hybrid hydrogel (HD-C) was prepared by integrating collagen I (Col I) and hyaluronic acid derivatives (HA-DN) with sulfhydryl modified polysaccharide hybrid hydrogel (HS-C) as control. Because of doub ...
DOI:
10.1016/j.carbpol.2021.118224

22. CRISPR activation of long non-coding RNA DANCR promotes bone regeneration

Author:
Nuong Thi Kieu Nguyen; Yu-Han Chang; Vu Anh Truong; Mu-Nung Hsu; Nam Ngoc Pham; Chin-Wei Chang; Yi-Hsiu Wu; Yi-Hao Chang; Hung Li; Yu-Chen Hu
Source:
Biomaterials 2021 v.275 pp. 120965
ISSN:
0142-9612
Subject:
Staphylococcus aureus; Streptococcus pyogenes; biocompatible materials; cartilage; chondrogenesis; non-coding RNA; rats; skull; therapeutics
Abstract:
... Healing of large calvarial bone defects in adults adopts intramembranous pathway and is difficult. Implantation of adipose-derived stem cells (ASC) that differentiate towards chondrogenic lineage can switch the bone repair pathway and improve calvarial bone healing. Long non-coding RNA DANCR was recently uncovered to promote chondrogenesis, but its roles in rat ASC (rASC) chondrogenesis and bone h ...
DOI:
10.1016/j.biomaterials.2021.120965

23. Calcium peroxide aids tyramine-alginate gel to crosslink with tyrosinase for efficient cartilage repair

Author:
Chin-Chean Wong; Chu-Xuan Lu; Er-Chen Cho; Po-Wei Lee; Nai-Wen Chi; Po-Yen Lin; Pei-Ru Jheng; Hsin-Lung Chen; Bradley W. Mansel; Yu-Ming Chen; Chih-Hwa Chen; Er-Yuan Chuang
Source:
International journal of biological macromolecules 2022 v.208 pp. 299-313
ISSN:
0141-8130
Subject:
alginates; bioadhesion; bioadhesives; calcium; calcium peroxide; cartilage; cell viability; chondrocytes; chondrogenesis; crosslinking; encapsulation; extracellular matrix; gels; oxygen; strength (mechanics); therapeutics; tyramine
Abstract:
... The innate cartilage extracellular matrix is avascular and plays a vital role in innate chondrocytes. Recapping the crucial components of the extracellular matrix in engineered organs via polymeric gels and bioinspired approaches is promising for improving the regenerative aptitude of encapsulated cartilage/chondrocytes. Conventional gel formation techniques for polymeric materials rely on employi ...
DOI:
10.1016/j.ijbiomac.2022.03.044

24. Carboxymethyl chitosan-assisted MnOx nanoparticles: Synthesis, characterization, detection and cartilage repair in early osteoarthritis

Author:
Ting Lin; Yang Zhao; Jieli Chen; Cuixi Wu; Zhan Li; Yumei Cao; Rong Lu; Jinwei Zhang; Chen Zhao; Yao Lu
Source:
Carbohydrate polymers 2022 v.294 pp. 119821
ISSN:
0144-8617
Subject:
biocompatibility; cartilage; chitosan; chondrogenesis; magnetism; manganese monoxide; osteoarthritis; peptides; rats; therapeutics
Abstract:
... While the early detection and repair of cartilage lesions are crucial in the treatment of osteoarthritis (OA), they remain challenging because neither clinically used medicines nor magnetic resonance (MR) contrast agents can achieve detection and repair simultaneously. Here, we conjugated carboxymethyl chitosan (CMC) with a cartilage-targeting peptide (WYRGRL, termed WY) and then synthesized CMC-a ...
DOI:
10.1016/j.carbpol.2022.119821

25. Cartilage from human-induced pluripotent stem cells: comparison with neo-cartilage from chondrocytes and bone marrow mesenchymal stromal cells

Author:
Alejandro Rodríguez Ruiz; Amanda Dicks; Margo Tuerlings; Koen Schepers; Melissa van Pel; Rob G. H. H. Nelissen; Christian Freund; Christine L. Mummery; Valeria Orlova; Farshid Guilak; Ingrid Meulenbelt; Yolande F. M. Ramos
Source:
Cell and tissue research 2021 v.386 no.2 pp. 309-320
ISSN:
0302-766X
Subject:
bone marrow; cartilage; chondrocytes; chondrogenesis; collagen; flow cytometry; humans; immunohistochemistry; medicine; research
Abstract:
... Cartilage has little intrinsic capacity for repair, so transplantation of exogenous cartilage cells is considered a realistic option for cartilage regeneration. We explored whether human-induced pluripotent stem cells (hiPSCs) could represent such unlimited cell sources for neo-cartilage comparable to human primary articular chondrocytes (hPACs) or human bone marrow-derived mesenchymal stromal cel ...
DOI:
10.1007/s00441-021-03498-5

26. Chitosan hydrogel/3D-printed poly(ε‐caprolactone) hybrid scaffold containing synovial mesenchymal stem cells for cartilage regeneration based on tetrahedral framework nucleic acid recruitment

Author:
Pinxue Li; Liwei Fu; Zhiyao Liao; Yu Peng; Chao Ning; Cangjian Gao; Daxu Zhang; Xiang Sui; Yunfeng Lin; Shuyun Liu; Chunxiang Hao; Quanyi Guo
Source:
Biomaterials 2021 v.278 pp. 121131
ISSN:
0142-9612
Subject:
DNA; biocompatible materials; cartilage; cell proliferation; chitosan; chondrogenesis; therapeutics
Abstract:
... Articular cartilage (AC) injury repair has always been a difficult problem for clinicians and researchers. Recently, a promising therapy based on mesenchymal stem cells (MSCs) has been developed for the regeneration of cartilage defects. As endogenous articular stem cells, synovial MSCs (SMSCs) possess strong chondrogenic differentiation ability and articular specificity. In this study, a cartilag ...
DOI:
10.1016/j.biomaterials.2021.121131

27. Chitosan/polycaprolactone multilayer hydrogel: A sustained Kartogenin delivery model for cartilage regeneration

Author:
Arezou Baharlou Houreh; Elahe Masaeli; Mohammad Hossein Nasr-Esfahani
Source:
International journal of biological macromolecules 2021 v.177 pp. 589-600
ISSN:
0141-8130
Subject:
cartilage; chitosan; chondrogenesis; gene expression; histology; hydrogels; hypertrophy; infrared spectroscopy; models; synergism; therapeutics
Abstract:
... Cartilage regeneration using biomaterial-guided delivery systems presents improved therapeutic efficacy of the biomolecules while minimizing side effects. Here, our hypothesis was to design a multilayer scaffold of chitosan (CS) hydrogel and polycaprolactone (PCL) mat to enhance the mechanical properties, integrity and stability of CS, especially for subsequent in vivo transplantation. After conju ...
DOI:
10.1016/j.ijbiomac.2021.02.122

28. Chondrocyte secretome enriched microparticles encapsulated with the chondrocyte membrane to facilitate the chondrogenesis of BMSCs and reduce hypertrophy

Author:
Zizhao Tang; Yan Lu; Shixin Zhang; Jing Wang; Qiguang Wang; Yumei Xiao; Xingdong Zhang
Source:
Journal of materials chemistry B 2021 v.9 no.48 pp. 9989-10002
ISSN:
2050-7518
Subject:
bone marrow; cartilage; cell proliferation; chemistry; chondrocytes; chondrogenesis; coculture; cytokines; encapsulation; gene expression; hypertrophy; phenotype; secretion
Abstract:
... Co-culture of chondrocytes and mesenchymal stem cells (MSCs) represents an effective way to stimulate the chondrogenesis of MSCs and reduce hypertrophy, but the limited donor site supply and the requirement of two-stage operations are among the major barriers of using autologous chondrocytes in clinical settings. With recent evidence indicating that the chondrogenic effects of the above co-culture ...
DOI:
10.1039/d1tb02319e

29. Citrate-based mussel-inspired magnesium whitlockite composite adhesives augmented bone-to-tendon healing

Author:
Xiaowei Yuan; Yitao Zhao; Jintao Li; Xuncai Chen; Zhihui Lu; Lianyong Li; Jinshan Guo
Source:
Journal of materials chemistry B 2021 v.9 no.39 pp. 8202-8210
ISSN:
2050-7518
Subject:
anterior cruciate ligament; bone formation; chemistry; chondrogenesis; magnesium
Abstract:
... Citrate-based mussel-inspired whitlockite composite adhesives (CMWAs) were developed and administered to the bone–tendon interface in anterior cruciate ligament (ACL) reconstruction. CMWAs could improve the initial bone–tendon bonding strength, promote the bony inward growth from the bone tunnel and enhance the chondrogenesis and osteogenesis of the bone–tendon interface, thus augmenting bone-to-t ...
DOI:
10.1039/d1tb01710a

30. Conditioned medium from mesenchymal stem cells improves condylar resorption induced by mandibular distraction osteogenesis in a rat model

Author:
Wataru Katagiri; Satoshi Endo; Ryoko Takeuchi; Daisuke Suda; Naoaki Saito; Tadaharu Kobayashi
Source:
Heliyon 2021 v.7 no.3 pp. e06530
ISSN:
2405-8440
Subject:
aesthetics; animal models; bone formation; cartilage; caudal vein; chondrogenesis; gene expression; head; micro-computed tomography; quantitative polymerase chain reaction; rats; resorption; surgery
Abstract:
... Condylar resorption (CR) after surgical orthognathic treatment is defined as dysfunctional remodeling of the temporomandibular joint manifested by morphological changes with decreased condylar head volume that cause occlusal and esthetic changes. Although both conservative and surgical treatment strategies have been employed for the treatment of CR, effective procedures have not been established t ...
DOI:
10.1016/j.heliyon.2021.e06530

31. Development of chimeric forms of the matrix metalloproteinase 2 collagen binding domain as artificial membrane binding proteins for targeting stem cells to cartilage lesions in osteoarthritic joints

Author:
Anais Dabbadie; Anna Salerno; Adam Perriman; Lu-Yun Lian; Anthony P. Hollander
Source:
Biomaterials 2022 v.285 pp. 121547
ISSN:
0142-9612
Subject:
amino acids; biocompatible materials; cartilage; chondrogenesis; collagen; gelatin; gelatinase A; ligands; solubility; stem cells
Abstract:
... Targeting stem cells to cartilage lesions has the potential to enhance engraftment and chondrogenesis. Denatured type II collagen fibrils (gelatin) are exposed in lesions at the surface of osteoarthritic articular cartilage and are therefore ideal target sites. We have designed and investigated chimeric mutants of the three modules of the MMP-2 collagen binding domain (CBD) as potential ligands fo ...
DOI:
10.1016/j.biomaterials.2022.121547

32. Dysregulated BMP signaling through ACVR1 impairs digit joint development in fibrodysplasia ossificans progressiva (FOP)

Author:
O. Will Towler; Sun H. Peck; Frederick S. Kaplan; Eileen M. Shore
Source:
Developmental biology 2021 v.470 pp. 136-146
ISSN:
0012-1606
Subject:
abnormal development; bone formation; bone morphogenetic proteins; cartilage; chondrogenesis; embryogenesis; genetic disorders; humans; mice; morphogenesis; mutation; phenotype; skeletal development; skeleton; stem cells
Abstract:
... The development of joints in the mammalian skeleton depends on the precise regulation of multiple interacting signaling pathways including the bone morphogenetic protein (BMP) pathway, a key regulator of joint development, digit patterning, skeletal growth, and chondrogenesis. Mutations in the BMP receptor ACVR1 cause the rare genetic disease fibrodysplasia ossificans progressiva (FOP) in which ex ...
DOI:
10.1016/j.ydbio.2020.11.004

33. Electrospun polycaprolactone/hydroxyapatite/ZnO films as potential biomaterials for application in bone-tendon interface repair

Author:
Xi He; Zongwang Huang; Wenbin Liu; Yanling Liu; Hu Qian; Ting Lei; Long Hua; Yihe Hu; Yi Zhang; Pengfei Lei
Source:
Colloids and surfaces 2021 v.204 pp. 111825
ISSN:
0927-7765
Subject:
biocompatible materials; bone formation; cell adhesion; chondrogenesis; hydroxyapatite; mice; tenocytes
Abstract:
... The bone-tendon interface (BTI) is a graded structure consisting of bone, mineralized and nonmineralized fibrocartilage, and tendons. Due to the complexity of the BTI structure, BTI healing is particularly challenging. To achieve a better material for BTI healing, polycaprolactone (PCL)/hydroxyapatite (HA)/ZnO films were constructed by the electrospinning method; in addition, the relevant material ...
DOI:
10.1016/j.colsurfb.2021.111825

34. Exosome-mediated delivery of kartogenin for chondrogenesis of synovial fluid-derived mesenchymal stem cells and cartilage regeneration

Author:
Xiao Xu; Yujie Liang; Xingfu Li; Kan Ouyang; Manyi Wang; Tong Cao; Wencui Li; Jianquan Liu; Jianyi Xiong; Biquan Li; Jiang Xia; Daping Wang; Li Duan
Source:
Biomaterials 2021 v.269 pp. 120539
ISSN:
0142-9612
Subject:
biocompatible materials; cartilage; chondrocytes; chondrogenesis; cytosol; exosomes; membrane proteins; models; osteoarthritis; peptides; rats; stem cells; therapeutics; water solubility
Abstract:
... Transplantation of synovial fluid-derived mesenchymal stem cells (SF-MSCs) is a viable therapy for cartilage degeneration of osteoarthritis (OA). But controlling chondrogenic differentiation of the transplanted SF-MSCs in the joints remains a challenge. Kartogenin (KGN) is a small molecule that has been discovered to induce differentiation of SF-MSCs to chondrocytes both in vitro and in vivo. The ...
DOI:
10.1016/j.biomaterials.2020.120539

35. Expression Profile of New Gene Markers Involved in Differentiation of Canine Adipose-Derived Stem Cells into Chondrocytes

Author:
Maurycy Jankowski; Mariusz Kaczmarek; Grzegorz Wąsiatycz; Aneta Konwerska; Claudia Dompe; Dorota Bukowska; Paweł Antosik; Paul Mozdziak; Bartosz Kempisty
Source:
Genes 2022 v.13 no.9 pp. -
ISSN:
2073-4425
Subject:
RNA; adipose tissue; cartilage; chondrocytes; chondrogenesis; diabetes; dogs; genes; heart diseases; medicine; models; osteoarthritis; spinal cord; stem cells; stroke; transcriptomics; veterinary clinics
Abstract:
... The interest in stem cell research continuously increased over the last decades, becoming one of the most important trends in the 21st century medicine. Stem cell-based therapies have a potential to become a solution for a range of currently untreatable diseases, such as spinal cord injuries, type I diabetes, Parkinson’s disease, heart disease, stroke, and osteoarthritis. Hence, this study, based ...
DOI:
10.3390/genes13091664

36. Fibrodysplasia Ossificans Progressiva: A Challenging Diagnosis

Author:
Daniele De Brasi; Francesca Orlando; Valeria Gaeta; Maria De Liso; Fabio Acquaviva; Luigi Martemucci; Augusto Mastrominico; Maja Di Rocco
Source:
Genes 2021 v.12 no.8 pp. -
ISSN:
2073-4425
Subject:
abnormal development; adrenal cortex hormones; bone formation; bone morphogenetic proteins; chondrogenesis; genes; heterozygosity; inflammation; phenotype; skeleton
Abstract:
... Fibrodysplasia ossificans progressiva (FOP) is an ultrarare genetic condition characterized by extraskeletal bone formation. Most of the musculoskeletal characteristics of FOP are related to dysregulated chondrogenesis, with heterotopic ossification being the most typical feature. Activating mutations of activin receptor A type I (ACVR1), a bone morphogenetic protein (BMP) type I receptor, are res ...
DOI:
10.3390/genes12081187

37. FoxO3a cooperates with RUNX1 to promote chondrogenesis and terminal hypertrophic of the chondrogenic progenitor cells

Author:
Shun Yuan; Lang Zhang; Lianru Ji; Sufang Zhong; Liyun Jiang; Yang Wan; Yonggui Song; Changhua Zhang; Rikang Wang
Source:
Biochemical and biophysical research communications 2022 v.589 pp. 41-47
ISSN:
0006-291X
Subject:
cartilage; chondrocytes; chondrogenesis; homeostasis; immunohistochemistry; neonates; osteoarthritis; osteoblasts; precipitin tests; research; therapeutics; transcription factors
Abstract:
... FoxO transcription factors (FoxOs) have recently been shown to protect against chondrocyte dysfunction and modulate cartilage homeostasis in osteoarthritis. The mechanism underlying of FoxOs regulate chondrocyte differentiation remains unknown. Runt related transcription factor 1 (RUNX1) mediated both chondrocyte and osteoblast differentiation. Our data showed that FoxO3a and RUNX1 are co-expresse ...
DOI:
10.1016/j.bbrc.2021.12.008

38. Genistein inhibits chondrogenic differentiation and mineralization of ATDC5 cells

Author:
Aya Kitagawa; Mana Yamaguchi; Aki Fujiwara; Muneshige Shimizu; Azusa Takahashi; Hideyuki Sone; Shin Kamiyama
Source:
Biochemical and biophysical research communications 2021 v.566 pp. 123-128
ISSN:
0006-291X
Subject:
Fabaceae; calcification; calcium; chondrogenesis; collagen; daidzein; genistein; mineralization; plant estrogens; proteoglycans; research
Abstract:
... Isoflavones are phytoestrogens abundant in leguminous crops and are used to prevent a variety of hormonal disorders. In the present study, the effects of genistein and daidzein on the chondrogenic differentiation of ATDC5 cells were investigated. Genistein (10 μM) treatment markedly reduced production of sulfated proteoglycans and collagen fibers in the ATDC5 cells. Genistein suppressed the expres ...
DOI:
10.1016/j.bbrc.2021.05.101

39. Highly effective rheumatoid arthritis therapy by peptide-promoted nanomodification of mesenchymal stem cells

Author:
Yao Lu; Zhan Li; Lihua Li; Jieli Chen; Xingyi Xu; Zefeng Lin; Tao Zhang; Ye Zhu; Changhai Ding; Chuanbin Mao
Source:
Biomaterials 2022 v.283 pp. 121474
ISSN:
0142-9612
Subject:
biocompatible materials; cartilage; catalase; cell movement; chondrogenesis; inflammation; intravenous injection; metformin; oxidation; peptides; rats; rheumatoid arthritis; stem cells; superoxide dismutase
Abstract:
... Traditional medication is not satisfied in rheumatoid arthritis (RA) therapy due to its long-term side effects and failure in cartilage repair. Nanomodification of mesenchymal stem cells (MSCs) holds promise for lifting such hurdles but delivering therapeutic nanomaterials (NPs) into MSCs remains challenging in this new strategy. Here, we show that CuS@MnO₂ NPs functionalized with a short phage-se ...
DOI:
10.1016/j.biomaterials.2022.121474

40. In situ biomimetic lyotropic liquid crystal gel for full-thickness cartilage defect regeneration

Author:
Hui Wang; Tingting Peng; Haofeng Wu; Jintian Chen; Minglong Chen; Liling Mei; Feng Li; Wenhao Wang; Chuanbin Wu; Xin Pan
Source:
Journal of controlled release 2021 v.338 pp. 623-632
ISSN:
0168-3659
Subject:
biomimetics; bones; cartilage; chondrogenesis; drug delivery systems; drugs; gels; histology; liquid crystals; lubrication; micro-computed tomography; pharmacodynamics; strength (mechanics)
Abstract:
... There is a great challenge in regenerating cartilage defects, which usually involve absent bearing capacity and poor adaptation to joint movement, further exacerbating subchondral bone damage. Therefore, ideal tissue-engineering cartilage scaffolds should be endowed with biomimetic and sustained-release function for promoting long-term chondrogenesis while protecting subchondral bone. Herein, in s ...
DOI:
10.1016/j.jconrel.2021.08.062

41. Integrated analysis of miRNA and mRNA transcriptomic reveals antler growth regulatory network

Author:
Boyin Jia; Linlin Zhang; Yifan Zhang; Chenxia Ge; Fuhe Yang; Rui Du; Hengxing Ba
Source:
Molecular genetics and genomics 2021 v.296 no.3 pp. 689-703
ISSN:
1617-4615
Subject:
bone formation; carcinogenesis; chondrogenesis; fibroblast growth factor receptor 1; genomics; microRNA; sequence analysis; transcriptomics; unigenes
Abstract:
... The growth of antler is driven by endochondral ossification in the growth center of the apical region. Antler grows faster than cancer tissues, but it can be stably regulated and regenerated periodically. To elucidate the molecular mechanisms of how antler grows rapidly without carcinogenesis, in this study, we used RNA-seq technology to evaluate the changes of miRNA and mRNA profiles in antler at ...
DOI:
10.1007/s00438-021-01776-z

42. Jellyfish Collagen: A Biocompatible Collagen Source for 3D Scaffold Fabrication and Enhanced Chondrogenicity

Author:
Zara Ahmed; Lydia C. Powell; Navid Matin; Andrew Mearns-Spragg; Catherine A. Thornton; Ilyas M. Khan; Lewis W. Francis
Source:
Marine drugs 2021 v.19 no.8 pp. -
ISSN:
1660-3397
Subject:
Rhizostoma; biocompatibility; biocompatible materials; bovine spongiform encephalopathy; cartilage; cattle; cell death; chondrogenesis; collagen; cytokines; extracellular matrix; histology; leukocytes; medicine; morbidity; osteoarthritis; strength (mechanics); swine
Abstract:
... Osteoarthritis (OA) is a multifactorial disease leading to degeneration of articular cartilage, causing morbidity in approximately 8.5 million of the UK population. As the dense extracellular matrix of articular cartilage is primarily composed of collagen, cartilage repair strategies have exploited the biocompatibility and mechanical strength of bovine and porcine collagen to produce robust scaffo ...
DOI:
10.3390/md19080405

43. Lizard Blastema Organoid Model Recapitulates Regenerated Tail Chondrogenesis

Author:
Ariel C. Vonk; Sarah C. Hasel-Kolossa; Gabriela A. Lopez; Megan L. Hudnall; Darian J. Gamble; Thomas P. Lozito
Source:
Journal of developmental biology 2022 v.10 no.1 pp. -
ISSN:
2221-3759
Subject:
Anolis carolinensis; Erinaceidae; cartilage; chondrogenesis; collagenase; dissociation; histology; hybridization; lizards; models; organoids; tail; tissue repair
Abstract:
... (1) Background: Lizard tail regeneration provides a unique model of blastema-based tissue regeneration for large-scale appendage replacement in amniotes. Green anole lizard (Anolis carolinensis) blastemas contain fibroblastic connective tissue cells (FCTCs), which respond to hedgehog signaling to create cartilage in vivo. However, an in vitro model of the blastema has not previously been achieved ...
DOI:
10.3390/jdb10010012

44. Long-term dynamic compression enhancement TGF-β3-induced chondrogenesis in bovine stem cells: a gene expression analysis

Author:
Jishizhan Chen; Lidan Chen; Jia Hua; Wenhui Song
Source:
BMC genomic data 2021 v.22 no.1 pp. 13
ISSN:
2730-6844
Subject:
Toll-like receptors; bioinformatics; cartilage; cattle; chondrogenesis; computer software; data collection; gene expression; gene expression regulation; gene ontology; genes; genomics; interleukin-6; protein-protein interactions; sensation
Abstract:
... BACKGROUND: Bioengineering has demonstrated the potential of utilising mesenchymal stem cells (MSCs), growth factors, and mechanical stimuli to treat cartilage defects. However, the underlying genes and pathways are largely unclear. This is the first study on screening and identifying the hub genes involved in mechanically enhanced chondrogenesis and their potential molecular mechanisms. METHODS: ...
DOI:
10.1186/s12863-021-00967-2

45. Oct4 facilitates chondrogenic differentiation of mesenchymal stem cells by mediating CIP2A expression

Author:
Lexiang Li; Qiwei Fu; Jiahua Shao; Bo Wang; Zheru Ding; Shuai Yuan; Jinhui Peng; Wei Xin; Jun Zhu; Yi Chen
Source:
Cell and tissue research 2022 v.389 no.1 pp. 11-21
ISSN:
0302-766X
Subject:
cartilage; chondrogenesis; gene expression regulation; microarray technology; phosphoprotein phosphatase; phosphorylation; quantitative polymerase chain reaction; research; skeletal development; transcription factors
Abstract:
... Bone development and cartilage formation require strict modulation of gene expression for mesenchymal stem cells (MSCs) to progress through their differentiation stages. Octamer-binding transcription factor 4 (Oct4) expression is generally restricted to developing embryonic pluripotent cells, but its role in chondrogenic differentiation (CD) of MSCs remains unclear. We therefore investigated the r ...
DOI:
10.1007/s00441-022-03619-8

46. Pharmaceutical therapeutics for articular regeneration and restoration: state-of-the-art technology for screening small molecular drugs

Author:
Yishan Chen; Heng Sun; Xudong Yao; Yeke Yu; Tian Tian; Weiyang Xu; Yujie Zhou; Hongwei Ouyang
Source:
Cellular and molecular life sciences 2021 v.78 no.24 pp. 8127-8155
ISSN:
1420-682X
Subject:
cartilage; chondrocytes; chondrogenesis; cost effectiveness; disease progression; epigenetics; inflammation; metabolism; osteoarthritis; phenotype; therapeutics
Abstract:
... Articular cartilage damage caused by sports injury or osteoarthritis (OA) has gained increased attention as a worldwide health burden. Pharmaceutical treatments are considered cost-effective means of promoting cartilage regeneration, but are limited by their inability to generate sufficient functional chondrocytes and modify disease progression. Small molecular chemical compounds are an abundant s ...
DOI:
10.1007/s00018-021-03983-8

47. Protein-reactive nanofibrils decorated with cartilage-derived decellularized extracellular matrix for osteochondral defects

Author:
Hye Sung Kim; Nandin Mandakhbayar; Hae-Won Kim; Kam W. Leong; Hyuk Sang Yoo
Source:
Biomaterials 2021 v.269 pp. 120214
ISSN:
0142-9612
Subject:
biocompatible materials; cartilage; cattle; chondrogenesis; epoxides; extracellular matrix; genes; mesenchymal stromal cells; nanofibers; polymerization; polymers; texture
Abstract:
... Cartilage defect is difficult to heal due to its avascular properties. Implantation of mesenchymal stem cell is one of the most promising approach for regenerating cartilage defects. Here we prepared polymeric nanofibrils decorated with cartilage-derived decellularized extracellular matrix (dECM) as a chondroinductive scaffold material for cartilage repair. To fabricate nanofibrils, eletrospun PCL ...
DOI:
10.1016/j.biomaterials.2020.120214

48. Single-cell profiles of human bone marrow-derived mesenchymal stromal cells after IFN-γ and TNF-α licensing

Author:
Shuanglong Lu; Xiaohong Qiao
Source:
Gene 2021 v.771 pp. 145347
ISSN:
0378-1119
Subject:
adipogenesis; bone formation; cell cycle; chemokines; chondrogenesis; gene expression; genes; genetic markers; humans; mesenchymal stromal cells; sequence analysis
Abstract:
... Pre-licensing mesenchymal stromal cells (MSCs) with IFN-γ and TNF-α can empower their immune fate and induce a more effective immune regulation. However, the cellular heterogeneity of MSCs limits our understanding of this inflammatory licensing.The publicly available Gene Expression Omnibus single-cell RNA sequencing (scRNA-seq) data of human bone marrow-derived MSCs with or without IFN-γ and TNF- ...
DOI:
10.1016/j.gene.2020.145347

49. Spatial organization of biochemical cues in 3D-printed scaffolds to guide osteochondral tissue engineering

Author:
Paula Camacho; Anne Behre; Matthew Fainor; Kelly B. Seims; Lesley W. Chow
Source:
Biomaterials science 2021 v.9 no.20 pp. 6813-6829
ISSN:
2047-4849
Subject:
biocompatible materials; bone formation; cartilage; chondrocytes; chondrogenesis; humans; hyaluronic acid; peptides; synergism; tissue repair
Abstract:
... Functional repair of osteochondral (OC) tissue remains challenging because the transition from bone to cartilage presents gradients in biochemical and physical properties necessary for joint function. Osteochondral regeneration requires strategies that restore the spatial composition and organization found in the native tissue. Several biomaterial approaches have been developed to guide chondrogen ...
DOI:
10.1039/d1bm00859e

50. Substrate stiffness and sequence dependent bioactive peptide hydrogels influence the chondrogenic differentiation of human mesenchymal stem cells

Author:
Mohiuddin Mohammed; Tsung-Sheng Lai; Hsin-Chieh Lin
Source:
Journal of materials chemistry B 2021 v.9 no.6 pp. 1676-1685
ISSN:
2050-7518
Subject:
amino acid sequences; cadherins; cartilage; cell culture; cell differentiation; chondrogenesis; glycoproteins; glycosaminoglycans; humans; hydrogels; nanofibers; pH; peptides; serine; stem cells
Abstract:
... N-Cadherin is a transmembrane glycoprotein that plays a crucial role in the condensation of mesenchymal cells by enhancing cell–cell interactions during the process of chondrogenesis. The biophysical and biochemical signals can incite enhanced cell–cell contact which ultimately determines the fate of stem cells. The role of cadherin mimetic peptides on the differentiation of mesenchymal stem cells ...
DOI:
10.1039/d0tb02008g

51. WNT5A-Ca2+-CaN-NFAT signalling plays a permissive role during cartilage differentiation in embryonic chick digit development

Author:
Alejandro Farrera-Hernández; Jessica Cristina Marín-Llera; Jesús Chimal-Monroy
Source:
Developmental biology 2021 v.469 pp. 86-95
ISSN:
0012-1606
Subject:
T-lymphocytes; calcium; cartilage; cell death; chicks; chondrogenesis; mice
Abstract:
... During digit development, the correct balance of chondrogenic signals ensures the recruitment of undifferentiated cells into the cartilage lineage or the maintenance of cells at the undifferentiated stage. WNT/β catenin maintains the pool of progenitor cells, whereas TGFβ signalling promotes cartilage differentiation by inducing Sox9 expression. Moreover, WNT5A promotes the degradation of β cateni ...
DOI:
10.1016/j.ydbio.2020.10.003

52. A chondroitin sulfate based injectable hydrogel for delivery of stem cells in cartilage regeneration

Author:
Xiaolin Li; Qian Xu; Melissa Johnson; Xi Wang; Jing Lyu; Yinghao Li; Sean McMahon; Udo Greiser; Sigen A; Wenxin Wang
Source:
Biomaterials science 2021 v.9 no.11 pp. 4139-4148
ISSN:
2047-4849
Subject:
biocompatibility; biocompatible materials; biopolymers; cartilage; cell viability; chondrogenesis; chondroitin sulfate; composite polymers; ethylene; gelation; hydrogels; inflammation; rats; stem cells; thiols
Abstract:
... Chondroitin sulfate (CS), as a popular material for cartilage tissue engineering scaffolds, has been extensively studied and reported for its safety and excellent biocompatibility. However, the rapid degradation of pure CS scaffolds has brought a challenge to regenerate neo-tissue similar to natural articular cartilage effectively. Meanwhile, the poly(ethene glycol) (PEG) -based biopolymer is freq ...
DOI:
10.1039/d1bm00482d

53. A dual functional chondro-inductive chitosan thermogel with high shear modulus and sustained drug release for cartilage tissue engineering

Author:
Dorsa Dehghan-Baniani; Babak Mehrjou; Dong Wang; Reza Bagheri; Atefeh Solouk; Paul K. Chu; Hongkai Wu
Source:
International journal of biological macromolecules 2022 v.205 pp. 638-650
ISSN:
0141-8130
Subject:
cartilage; chitosan; chondrogenesis; diclofenac; drugs; gelation; halloysite; hydrogels; inflammation; microparticles; modulus of elasticity; nanocomposites; nanotubes; starch
Abstract:
... We report a chitosan-based nanocomposite thermogel with superior shear modulus resembling that of cartilage and dual pro-chondrogenic and anti-inflammatory functions. Two therapeutic agents, kartogenin (KGN) and diclofenac sodium (DS), are employed to promote chondrogenesis of stem cells and suppress inflammation, respectively. To extend the release time in a controlled manner, KGN is encapsulated ...
DOI:
10.1016/j.ijbiomac.2022.02.115

54. A highly porous type II collagen containing scaffold for the treatment of cartilage defects enhances MSC chondrogenesis and early cartilaginous matrix deposition

Author:
Claudio Intini; Mark Lemoine; Tom Hodgkinson; Sarah Casey; John P. Gleeson; Fergal J. O'Brien
Source:
Biomaterials science 2022 v.10 no.4 pp. 970-983
ISSN:
2047-4849
Subject:
biocompatible materials; biomimetics; cartilage; chondrogenesis; collagen; hyaluronic acid; mesenchymal stromal cells; porosity; tissue repair
Abstract:
... A major challenge in cartilage tissue engineering (TE) is the development of instructive and biomimetic scaffolds capable of driving effective mesenchymal stem cell (MSC) chondrogenic differentiation and robust de novo matrix formation. Type I collagen-based scaffolds are one of the most commonly selected materials given collagen's intrinsic ability to act as an instructive and active biomaterial. ...
DOI:
10.1039/d1bm01417j

55. A hydrogel/particle composite with a gradient of oxygen releasing microparticle for concurrent osteogenic and chondrogenic differentiation in a single scaffold

Author:
Sajedeh Khorshidi; Reza Karimi-Soflou; Akbar Karkhaneh
Source:
Colloids and surfaces 2021 v.207 pp. 112007
ISSN:
0927-7765
Subject:
bone formation; calcium; calcium peroxide; cell culture; cell viability; chondrogenesis; face; fibroins; gene expression; humans; hydrogels; microparticles; oxidation; oxygen; pectins; polylactic acid; toxicity
Abstract:
... In the present study, a hydrogel/particle scaffold with a gradient of the oxygen releasing microparticles was developed. Hydrogel component was composed of the oxidized pectin and silk fibroin, whereas the microparticles were constituted from polylactic acid (PLA) and calcium peroxide (CPO). A controlled mixing of the suspensions with different content of the PLA/CPO microparticles conferred a gra ...
DOI:
10.1016/j.colsurfb.2021.112007

56. The impact of photobiomodulation on the chondrogenic potential of adipose-derived stromal/stem cells

Author:
C. Schneider; P. Dungel; E. Priglinger; M. Danzer; B. Schädl; S. Nürnberger
Source:
Journal of photochemistry and photobiology 2021 v.221 pp. 112243
ISSN:
1011-1344
Subject:
DNA; cartilage; chondrogenesis; collagen; cost effectiveness; gene expression; histology; humans; phenotype; photobiology; photochemistry; red light; tissue repair
Abstract:
... Due to their capacity to differentiate into the chondrogenic lineage, adipose-derived stromal/stem cells (ASC) are a promising source of therapeutically relevant cells for cartilage tissue regeneration. Their differentiation potential, however, varies between patients. In our study, we aim to stimulate ASC towards a more reliable chondrogenic phenotype using photobiomodulation (PBM).LED devices of ...
DOI:
10.1016/j.jphotobiol.2021.112243

57. The lower in vitro chondrogenic potential of canine adipose tissue-derived mesenchymal stromal cells (MSC) compared to bone marrow-derived MSC is not improved by BMP-2 or BMP-6

Author:
M. Teunissen; F. Verseijden; F.M. Riemers; G.J.V.M. van Osch; M.A. Tryfonidou
Source:
veterinary journal 2021 v.269 pp. 105605
ISSN:
1090-0233
Subject:
chondrogenesis; dogs; flow cytometry; humans; osteoarthritis
Abstract:
... Mesenchymal stromal cells (MSC) are used for cell-based treatment for canine osteoarthritis (OA). Compared with human MSCs, detailed information on the functional characterisation of canine MSCs is limited. In particular, the chondrogenic differentiation of canine adipose tissue-derived MSCs (cAT-MSCs) is challenging. In this study, we aimed to compare cAT-MSCs with bone marrow-derived MSCs (cBM-M ...
DOI:
10.1016/j.tvjl.2020.105605

58. 3D-bioprinted BMSC-laden biomimetic multiphasic scaffolds for efficient repair of osteochondral defects in an osteoarthritic rat model

Author:
Yanzhi Liu; Liuqi Peng; Lingli Li; Cuishan Huang; Keda Shi; Xiangbo Meng; Pinpin Wang; Mingming Wu; Ling Li; Huijuan Cao; Kefeng Wu; Qingqiang Zeng; Haobo Pan; William Weijia Lu; Ling Qin; Changshun Ruan; Xinluan Wang
Source:
Biomaterials 2021 v.279 pp. 121216
ISSN:
0142-9612
Subject:
animal models; biocompatible materials; biomimetics; cartilage; chondrogenesis; collagen; diclofenac; extracellular matrix; gait; hyaluronic acid; inflammation; mesenchymal stromal cells; osteoarthritis
Abstract:
... Osteochondral defect repair in osteoarthritis (OA) remains an unsolved clinical problem due to the lack of enough seed cells in the defect and chronic inflammation in the joint. To address this clinical need, we designed a bone marrow-derived mesenchymal stem cell (BMSC)-laden 3D-bioprinted multilayer scaffold with methacrylated hyaluronic acid (MeHA)/polycaprolactone incorporating kartogenin and ...
DOI:
10.1016/j.biomaterials.2021.121216

59. ATDC5 cells as a model of cartilage extracellular matrix neosynthesis, maturation and assembly

Author:
Dafné Wilhelm; Hervé Kempf; Arnaud Bianchi; Jean-Baptiste Vincourt
Source:
Journal of proteomics 2020 v.219 pp. 103718
ISSN:
1874-3919
Subject:
biosynthesis; cartilage; cell lines; chondrogenesis; collagen; extracellular matrix; models; post-translational modification; proteome
Abstract:
... Fibrillar collagens and proteoglycans (PGs) are quantitatively the major constituents of extracellular matrices (ECM). They carry numerous crucial post-translational modifications (PTMs) that tune the resulting biomechanical properties of the corresponding tissues. The mechanisms determining these PTMs remain largely unknown, notably because available established cell lines do not recapitulate muc ...
DOI:
10.1016/j.jprot.2020.103718

60. Advanced gellan gum-based glycol chitosan hydrogel for cartilage tissue engineering biomaterial

Author:
Sumi Lee; Joo Hee Choi; Ain Park; Mina Rim; Jina Youn; Wonchan Lee; Jeong Eun Song; Gilson Khang
Source:
International journal of biological macromolecules 2020 v.158 pp. 452-460
ISSN:
0141-8130
Subject:
biocompatibility; biocompatible materials; biodegradability; biopolymers; cartilage; cell adhesion; chitosan; chondrogenesis; extracellular matrix; gellan gum; histology; hydrogels; in vitro studies; mechanical properties; models; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; tissue engineering; viability assays; water solubility; weight loss
Abstract:
... Gellan gum (GG), a nature-derived polysaccharide, is one of the materials widely used in cartilage tissue engineering (TE). Glycol chitosan (GC), a derivative of chitosan, is a water-soluble natural polymer that has excellent biocompatibility and biodegradability as well as cell adhesion. Herein, GG was physically blended with GC to enhance the mechanical properties and microenvironment of the GG ...
DOI:
10.1016/j.ijbiomac.2020.04.135

61. Aqueous extract of Arctium lappa L. root (burdock) enhances chondrogenesis in human bone marrow-derived mesenchymal stem cells

Author:
King-Chuen Wu; Hung-Kai Weng; Yun-Shang Hsu; Pin-Jia Huang; Yang-Kao Wang
Source:
BMC complementary medicine and therapies 2020 v.20 no.1 pp. 364
ISSN:
2662-7671
Subject:
Arctium lappa; Oriental traditional medicine; adipogenesis; alternative medicine; bone formation; cell proliferation; chondrogenesis; collagen; culture media; dose response; humans; mesenchymal stromal cells
Abstract:
... BACKGROUND: Arctium lappa L. root (burdock root) has long been recommended for the treatment of different diseases in traditional Chinese medicine. Burdock root possesses anti-oxidative, anti-inflammatory, anti-cancer, and anti-microbial activities. The aim of the study was to elucidate whether aqueous extract of burdock root regulates mesenchymal stem cell proliferation and differentiation. METHO ...
DOI:
10.1186/s12906-020-03158-1

62. Bioinspired poly (γ-glutamic acid) hydrogels for enhanced chondrogenesis of bone marrow-derived mesenchymal stem cells

Author:
Rong Yang; Xiaoxue Wang; Shuai Liu; Wenjie Zhang; Penghui Wang; Xin Liu; Yanhan Ren; Xiaoyan Tan; Bo Chi
Source:
International journal of biological macromolecules 2020 v.142 pp. 332-344
ISSN:
0141-8130
Subject:
biocompatible materials; biomimetics; cartilage; chondrogenesis; cysteamine; hydrogels; mechanical properties; mechanical stress; mesenchymal stromal cells; models; moieties; polyglutamic acid; rabbits; rheological properties; stem cells; tissue repair
Abstract:
... While peptide-directed scaffolds now serve as well-established platforms for biomimetic three-dimensional (3D) extracellular matrices (ECM), challenges still remain for chondrogenesis through direct mediation of stem cells. Here, biocompatible poly (γ-glutamic acid) (γ-PGA) hydrogels with robust mechanical properties were developed based on methacrylate-γ-PGA (γ-PGA-GMA) and cysteamine functionali ...
DOI:
10.1016/j.ijbiomac.2019.09.104

63. Biomimetic poly(γ-glutamic acid) hydrogels based on iron (III) ligand coordination for cartilage tissue engineering

Author:
Penghui Wang; Wenjie Zhang; Rong Yang; Shuai Liu; Yanhan Ren; Xin Liu; Xiaoyan Tan; Bo Chi
Source:
International journal of biological macromolecules 2021 v.167 pp. 1508-1516
ISSN:
0141-8130
Subject:
biomimetics; cartilage; chondrogenesis; extracellular matrix; gene expression; glycosaminoglycans; hydrogels; hydroxyproline; iron; ligands; research and development; secretion
Abstract:
... For the problems in the research on differentiation of mesenchymal stem cells (BMSCs), such as poor differentiation tendency and low differentiation efficiency, a novel photo-crosslinked extracellular matrix (ECM) inspired double network hydrogel that composed of poly(γ-glutamic acid) (γ-PGA) hydrogel and Fe³⁺ ligand coordination was designed and manufactured. Compared with those traditional γ-PGA ...
DOI:
10.1016/j.ijbiomac.2020.11.105

64. Childhood Cartilage ECM Enhances the Chondrogenesis of Endogenous Cells and Subchondral Bone Repair of the Unidirectional Collagen–dECM Scaffolds in Combination with Microfracture

Author:
Hongfu Cao; Xiuyu Wang; Manyu Chen; Yuhan Liu; Xiaolin Cui; Jie Liang; Qiguang Wang; Yujiang Fan; Xingdong Zhang
Source:
ACS applied materials & interfaces 2021 v.13 no.48 pp. 57043-57057
ISSN:
1944-8252
Subject:
DNA; adolescents; cartilage; cell structures; childhood; chondrogenesis; extracellular matrix; protein synthesis; rabbits; therapeutics
Abstract:
... Despite the formation of mechanically inferior fibrocartilage, microfracture (MF) still remains the gold standard to repair the articular cartilage defects in clinical settings. To date, although many tissue-engineering scaffolds have been developed to enhance the MF outcome, the clinical outcomes remain inconsistent. Decellularized extracellular matrix (dECM) is among the most promising scaffold ...
DOI:
10.1021/acsami.1c19447

65. Chondrogenesis of cocultures of mesenchymal stem cells and articular chondrocytes in poly(l-lysine)-loaded hydrogels

Author:
Yu Seon Kim; Athena J. Chien; Jason L. Guo; Brandon T. Smith; Emma Watson; Hannah A. Pearce; Gerry L. Koons; Adam M. Navara; Johnny Lam; David W. Scott; K. Jane Grande-Allen; Antonios G. Mikos
Source:
Journal of controlled release 2020 v.328 pp. 710-721
ISSN:
0168-3659
Subject:
chondrocytes; chondrogenesis; chondroitin; coculture; collagen; encapsulation; histology; hydrogels; long term effects; lysine; mesenchymal stromal cells; models; molecular weight; phenotype; viability
Abstract:
... This work investigated the effect of poly(l-lysine) (PLL) molecular weight and concentration on chondrogenesis of cocultures of mesenchymal stem cells (MSCs) and articular chondrocytes (ACs) in PLL-loaded hydrogels. An injectable dual-network hydrogel composed of a poly(N-isopropylacrylamide)-based synthetic thermogelling macromer and a chondroitin sulfate-based biological network was leveraged as ...
DOI:
10.1016/j.jconrel.2020.09.048

66. Chondrogenic differentiation followed IGFBP3 loss in human endometrial mesenchymal stem cells

Author:
Roman E. Ushakov; Elena V. Skvortsova; Mikhail A. Vitte; Irina O. Vassilieva; Alla N. Shatrova; Anastasiya V. Kotova; Vladimir M. Kenis; Elena B. Burova
Source:
Biochemical and biophysical research communications 2020 v.531 no.2 pp. 133-139
ISSN:
0006-291X
Subject:
CRISPR-Cas systems; apoptosis; cell growth; chondrogenesis; collagen; endometrium; humans; research; somatomedins
Abstract:
... Insulin-like growth factor binding protein 3 (IGFBP3) is a multifunctional protein, able either to stimulate the cell growth or to promote apoptosis. In particular, IGFBP3 plays significant role in propagation of stress-induced senescence in human endometrium-derived mesenchymal stem cells (MESCs) (Vassilieva et al., 2020). We undertook CRISPR/Cas9-mediated IGFBP3 knockout in an effort to decelera ...
DOI:
10.1016/j.bbrc.2020.07.064

67. Chondrogenic differentiation of mesenchymal stem/stromal cells on 3D porous poly (ε-caprolactone) scaffolds: Effects of material alkaline treatment and chondroitin sulfate supplementation

Author:
Carla Sofia Moura; João Carlos Silva; Sofia Faria; Paulo Rui Fernandes; Cláudia Lobato da Silva; Joaquim Manuel Sampaio Cabral; Robert Linhardt; Paulo Jorge Bártolo; Frederico Castelo Ferreira
Source:
Journal of bioscience and bioengineering 2020 v.129 no.6 pp. 756-764
ISSN:
1389-1723
Subject:
alkali treatment; biocompatible materials; bone marrow; cartilage; cell adhesion; chondrogenesis; chondroitin sulfate; gene expression; humans; hydrophilicity; lactones; mechanical properties; micro-computed tomography; osteoarthritis; oxygen; porosity; sodium hydroxide; stem cells; stromal cells; surface area; tissue engineering; wettability
Abstract:
... Cartilage defects resultant from trauma or degenerative diseases (e.g., osteoarthritis) can potentially be repaired using tissue engineering (TE) strategies combining progenitor cells, biomaterial scaffolds and bio-physical/chemical cues. This work examines promoting chondrogenic differentiation of human bone marrow mesenchymal stem/stromal cells (BM-MSCs) by combining the effects of modified poly ...
DOI:
10.1016/j.jbiosc.2020.01.004

68. Direct Visualization of the Binding of Transforming Growth Factor Beta 1 with Cartilage Oligomeric Matrix Protein via High-Resolution Atomic Force Microscopy

Author:
Victoria Tran; Arpad Karsai; Michael C. Fong; Weiliang Cai; J. Gabriel Fraley; Jasper H.N. Yik; Eric Klineberg; Dominik R. Haudenschild; Gang-yu Liu
Source:
Journal of physical chemistry 2020 v.124 no.43 pp. 9497-9504
ISSN:
1520-5207
Subject:
atomic force microscopy; cartilage; chondrogenesis
Abstract:
... This work reports the first direct observations of binding and complex formation between transforming growth factor beta 1 (TGF-β1) and cartilage oligomeric matrix protein (COMP) using high-resolution atomic force microscopy (AFM). Each COMP molecule consists of pentamers whose five identical monomeric units bundle at N-termini. From this central point, the five monomers’ flexible arms extend outw ...
DOI:
10.1021/acs.jpcb.0c07286

69. Dynamic loading enhances chondrogenesis of human chondrocytes within a biodegradable resilient hydrogel

Author:
Mingsheng Xie; Madalyn Fritch; Yuchen He; Huikang Fu; Yi Hong; Hang Lin
Source:
Biomaterials science 2021 v.9 no.14 pp. 5011-5024
ISSN:
2047-4849
Subject:
biocompatible materials; biodegradability; cartilage; chondrocytes; chondrogenesis; humans; hydrogels; phosphotransferases (kinases); polyethylene; transient receptor potential vanilloid channels; viability
Abstract:
... Hyaline cartilage in the knee joint is a soft tissue that is both stiff and elastic, which raises unique challenges in developing scaffolds for the repair of cartilage injury. In this study, we mixed poly-d,l-lactic acid/polyethylene glycol/poly-d,l-lactic acid (PEG-PDLLA-DA) with polycaprolactone–poly(ethylene glycol)-polycaprolactone (PEG-PCL-DA) with the aim to create a cartilage-like hydrogel. ...
DOI:
10.1039/d1bm00413a

70. Effect of quercetin on chondrocyte phenotype and extracellular matrix expression

Author:
Zhi-Peng GUI; Yue HU; Yu-Ning ZHOU; Kai-Li LIN; Yuan-Jin XU
Source:
Chinese journal of natural medicines 2020 v.18 no.12 pp. 922-933
ISSN:
1875-5364
Subject:
Oriental traditional medicine; Western blotting; assays; cartilage; chondrogenesis; dose response; extracellular matrix; fluorescent antibody technique; genes; glycosaminoglycans; histology; in vivo studies; liver; phenotype; quercetin; rats; scaffolding proteins; secretion; signal transduction; silk proteins; staining; tissue repair
Abstract:
... Due to the poor repair ability of cartilage tissue, regenerative medicine still faces great challenges in the repair of large articular cartilage defects. Quercetin is widely applied as a traditional Chinese medicine in tissue regeneration including liver, bone and skin tissues. However, the evidence for its effects and internal mechanisms for cartilage regeneration are limited. In the present stu ...
DOI:
10.1016/S1875-5364(20)60036-X

71. Endothelin-1 reduces catabolic activity of human mesenchymal stem/stromal cells during chondro- and osteo-lineage differentiation

Author:
Robert Weishar; Ming-Song Lee; Gianluca Fontana; Peiman Hematti; Wan-Ju Li
Source:
Biochemical and biophysical research communications 2020 v.529 no.2 pp. 180-185
ISSN:
0006-291X
Subject:
alizarin; blood vessels; bone formation; cell proliferation; chondrogenesis; endothelins; extracellular matrix; humans; research
Abstract:
... Human mesenchymal stem/stromal cells (hMSCs) reside in a vascularized microenvironment and experience a host of blood vessel secretions, including endothelin-1 (ET1). Previously, our group has demonstrated improved induction of osteogenesis and chondrogenesis in hMSCs through an ET1-induced increase in production of anabolic factors. The current study explores effects of ET1 on catabolic factors s ...
DOI:
10.1016/j.bbrc.2020.06.003

72. Evaluation of alginate modification effect on cell-matrix interaction, mechanotransduction and chondrogenesis of encapsulated MSCs

Author:
Azadeh Jahanbakhsh; Mohammad Sadegh Nourbakhsh; Shahin Bonakdar; Mohammad Ali Shokrgozar; Nooshin Haghighipour
Source:
Cell and tissue research 2020 v.381 no.2 pp. 255-272
ISSN:
0302-766X
Subject:
alginates; cartilage; cell differentiation; chondrogenesis; collagen; encapsulation; extracellular matrix; gelatin; genes; hydrogels; hydrostatic pressure; immunohistochemistry; mechanotransduction; mesenchymal stromal cells; methylene blue; phenotype; quantitative polymerase chain reaction; research; staining; synergism; transforming growth factor beta
Abstract:
... Mesenchymal stem cells (MSCs) are promising cell candidates for cartilage regeneration. Furthermore, it is important to control the cell-matrix interactions that have a direct influence on cell functions. Providing an appropriate microenvironment for cell differentiation in response to exogenous stimuli is a critical step towards the clinical utilization of MSCs. In this study, hydrogels consisted ...
DOI:
10.1007/s00441-020-03216-7

73. Expression and function of Ebf1 gene during chondrogenesis in chick embryo limb buds

Author:
Mohammed A. El-Magd; Ahmed Abdelfattah-Hassan; Rasha A. Elsisy; Yousef M Hawsawi; Atif A. Oyouni; Osama M. Al-Amer; Eman S. El-Shetry
Source:
Gene 2021 v.803 pp. 145895
ISSN:
0378-1119
Subject:
B-lymphocytes; alkaline phosphatase; chick embryos; chicks; chondrocytes; chondrogenesis; collagen; genes; phenotype
Abstract:
... The expression profile of early B-cell factor (Ebf) genes and loss of function experiments denote a crucial role for these genes during the late stage of skeletogenesis. However, little is known regarding the expression and function of these genes during the early stage of skeletogenesis. Therefore, this study aimed to detail the spatiotemporal expression pattern of cEbf1, in comparison to cEbf2 a ...
DOI:
10.1016/j.gene.2021.145895

74. FLASH: Fluorescently LAbelled Sensitive Hydrogel to monitor bioscaffolds degradation during neocartilage generation

Author:
Carmine Onofrillo; Serena Duchi; Sam Francis; Cathal D. O'Connell; Lilith M. Caballero Aguilar; Stephanie Doyle; Zhilian Yue; Gordon G. Wallace; Peter F. Choong; Claudia Di Bella
Source:
Biomaterials 2021 v.264 pp. 120383
ISSN:
0142-9612
Subject:
biocompatible materials; cartilage; chemical bonding; chondrogenesis; ex vivo studies; extracellular matrix; fluorescence; fluorescent dyes; gelatin; humans; hydrogels
Abstract:
... Regenerative therapies based on photocrosslinkable hydrogels and stem cells are of growing interest in the field of cartilage repair. Cell-mediated degradation is critical for the successful clinical translation of implanted hydrogels. However, characterising cell-mediated degradation, while simultaneously monitoring the deposition of a distinct new matrix, remains a major challenge.In this study ...
DOI:
10.1016/j.biomaterials.2020.120383

75. Feasibility of bone marrow mesenchymal stem cells harvesting from forearm bone

Author:
Tulyapruek Tawonsawatruk; Thepparat Kanchanathepsak; Thitinat Duangchan; Kuneerat Aswamenakul; Aungkura Supokawej
Source:
Heliyon 2021 v.7 no.7 pp. e07639
ISSN:
2405-8440
Subject:
arms (limbs); bone formation; bone marrow; chondrogenesis; growth models; isolation techniques; mesenchymal stromal cells; orthopedics; surgery; ulna
Abstract:
... Mesenchymal stem cell is a promising therapeutic option in orthopedic filed and regenerative medicine. The feasibility of isolation method and characterization of Mesenchymal stem cell including growth kinetics, immunophenotypes and differentiation potency from small volume aspiration harvested from ulna and radius should be evaluated in order to utilize this cell in hand surgery.Mesenchymal stem ...
DOI:
10.1016/j.heliyon.2021.e07639

76. Fibronectin Bound to a Fibrous Substrate Has Chondrogenic Induction Properties

Author:
Marta R. Casanova; Rui L. Reis; Albino Martins; Nuno M. Neves
Source:
Biomacromolecules 2020 v.21 no.4 pp. 1368-1378
ISSN:
1526-4602
Subject:
antibodies; blood plasma; cartilage; chondrogenesis; collagen; culture media; extracellular matrix; fibronectins; humans; mesenchymal stromal cells; nanofibers; tissue engineering
Abstract:
... Articular cartilage is an avascular tissue characterized by a dense and specific extracellular matrix (ECM). Fibronectin (FN) is a key constituent of the pericellular ECM, assembled into a fibrillar matrix through a cell-mediated process, being implicated in chondrogenic events. In this study, we evaluate the chondrogenic potential of FN bound to the surface of an electrospun nanofibrous mesh (NFM ...
DOI:
10.1021/acs.biomac.9b01546

77. Glycosylation of DMP1 promotes bone reconstruction in long bone defects

Author:
Hui Xue; Pingping Niu; Yang Liu; Yao Sun
Source:
Biochemical and biophysical research communications 2020 v.526 no.4 pp. 1125-1130
ISSN:
0006-291X
Subject:
bone formation; callus; chondrogenesis; collagen; cytokines; glycosylation; mice; models; mutants; proteoglycans; research; strength (mechanics)
Abstract:
... The regeneration of bone defects is necessary for the successful healing. During the process of healing, callus plays crucial roles in providing the stable bone-reconstruction environment. The callus is consisted of various large molecules including collagen proteins, non-collagen proteins and proteoglycans (PGs), which are involved in maintaining mechanical strength and interacting with cytokines ...
DOI:
10.1016/j.bbrc.2020.04.020

78. HBC-nanofiber hydrogel scaffolds with 3D printed internal microchannels for enhanced cartilage differentiation

Author:
Xiaoyun Liu; Shaoshuai Song; Jie Huang; Han Fu; Xinyu Ning; Yong He; Zhijun Zhang
Source:
Journal of materials chemistry B 2020 v.8 no.28 pp. 6115-6127
ISSN:
2050-7518
Subject:
biomimetics; cartilage; cell growth; chitosan; chondrogenesis; gelation; gene expression; humans; hydrogels; nanofibers; orthopedics; three-dimensional printing
Abstract:
... Articular cartilage injuries are a major orthopedic problem. Cartilage repair is a long-standing challenge due to the limited self-regenerative capability of cartilage. Tissue engineering offers a new and effective approach to cartilage repair. We report herein the fabrication of 3D scaffolds that mimic the native structure of cartilage, by first preparing poly(lactic-co-glycolic acid) (PLGA) elec ...
DOI:
10.1039/d0tb00616e

79. Hyperosmolarity benefits cartilage regeneration by enhancing expression of chondrogenic markers and reducing inflammatory markers

Author:
Sepideh Alinezhad-Bermi; Mahboubeh Kabiri; Iman Rad; Shiva Irani; Hana Hanaee-Ahvaz
Source:
In vitro cellular & developmental biology 2021 v.57 no.3 pp. 290-299
ISSN:
1071-2690
Subject:
cartilage; chondrogenesis; glycosaminoglycans; immunoassays; immunocytochemistry; mineralization; secretion; therapeutics
Abstract:
... Application of hyperosmolarity can be a promising strategy to promote chondrogenic differentiation in adipose-derived mesenchymal stem cells (ADSCs). Growth factors may promote different signaling pathways in parallel that is why in this study we monitor undesired pathologic or unwanted side effects as well as chondroinductive impacts of hyperosmolarity in differentiating ADSCs. Quantified gene ex ...
DOI:
10.1007/s11626-020-00430-z

80. Hypoxia during incubation and its effects on broiler's embryonic development

Author:
Amit Haron; Mark Ruzal; Dmitry Shinder; Shelly Druyan
Source:
Poultry science 2021 v.100 no.3 pp. 100951
ISSN:
0032-5791
Subject:
angiogenesis; chicks; chondrogenesis; chorioallantoic membrane; dose response; embryogenesis; fetal development; hematopoiesis; hypoxia; oxygen
Abstract:
... In all vertebrates, hypoxia plays an important role in fetal development, driving vasculogenesis, angiogenesis, hematopoiesis, and chondrogenesis. Therefore, the ability to sense and respond to changes in the availability of oxygen (O₂) is crucial for normal embryonic development as well as for developmental plasticity. Moderate levels of hypoxia trigger a regulated process which leads to adaptive ...
DOI:
10.1016/j.psj.2020.12.048

81. In situ ornamenting poly(ε-caprolactone) electrospun fibers with different fiber diameters using chondrocyte-derived extracellular matrix for chondrogenesis of mesenchymal stem cells

Author:
Jiazhu Xu; Qi Fang; Yanli Liu; Yan Zhou; Zhaoyang Ye; Wen-Song Tan
Source:
Colloids and surfaces 2021 v.197 pp. 111374
ISSN:
0927-7765
Subject:
adhesion; biomimetics; cartilage; chondrocytes; chondrogenesis; collagen; extracellular matrix; gene expression; nanofibers; tissue repair
Abstract:
... Biomimetic instructive tissue engineering scaffolds are critical for achieving successful tissue regeneration. In the present study, we developed a novel scaffold via ornamenting poly(ε-caprolactone) (PCL) electrospun fibers with a chondrocyte-derived extracellular matrix (ECM)-coating, which was applied for chondrogenesis of mesenchymal stem cells (MSCs). PCL fibrous films with different fiber di ...
DOI:
10.1016/j.colsurfb.2020.111374

82. Injectable in situ forming kartogenin-loaded chitosan hydrogel with tunable rheological properties for cartilage tissue engineering

Author:
Dorsa Dehghan-Baniani; Yin Chen; Dong Wang; Reza Bagheri; Atefeh Solouk; Hongkai Wu
Source:
Colloids and surfaces 2020 v.192 pp. 111059
ISSN:
0927-7765
Subject:
ambient temperature; biocompatible materials; cartilage; chitosan; chondrogenesis; drugs; gelation; humans; hydrogels; mechanical properties; mesenchymal stromal cells; modulus of elasticity; response surface methodology; statistical models; stem cells; thermosensitivity; tissue engineering
Abstract:
... Limited regeneration capacity of cartilage can be addressed by tissue engineering approaches including localized delivery of bioactive agents using biomaterials. Although chitosan hydrogels have been considered as appropriate candidates for these purposes, however, their poor mechanical properties limit their real applications. Here, we develop in situ forming chitosan hydrogels with enhanced shea ...
DOI:
10.1016/j.colsurfb.2020.111059

83. Lkb1 is an important regulator of Treg differentiation and proliferation of amniotic mesenchymal stem cells

Author:
Maolan Liu; Jiadi Chen; Huifang Huang; Yun Zeng; Xiaoming Feng; Mingxia Shi
Source:
Biochemical and biophysical research communications 2020 v.521 no.2 pp. 434-440
ISSN:
0006-291X
Subject:
Western blotting; apoptosis; bone formation; chondrogenesis; coculture; fibroblasts; flow cytometry; immunosuppression; liver; phenotype; research; surface antigens
Abstract:
... In this study, we aimed to explore the role of liver kinase b1 (Lkb1) in the biological characteristics and immune regulation of amniotic mesenchymal stem cells (AMSCs). AMSCs were identified via the cell surface markers using flow cytometry. We knocked down the expression of Lkb1 in AMSCs using lentivirus-mediated Lkb1-specific shRNA. The efficiency of the knockdown was detected by flow cytometry ...
DOI:
10.1016/j.bbrc.2019.09.129

84. Mechanism of TGF-β3 promoting chondrogenesis in human fat stem cells

Author:
Debao Li; Xiaofei Ma; Tianlan Zhao
Source:
Biochemical and biophysical research communications 2020 v.530 no.4 pp. 725-731
ISSN:
0006-291X
Subject:
cartilage; chondrocytes; chondrogenesis; flow cytometry; fluorescence microscopy; humans; immunohistochemistry; research; stem cells
Abstract:
... Clinically deficient cartilage is difficult to regenerate, and the availability of chondrocytes is very limited. However, human adipose-derived stem cells (ADSCs) can be obtained easily and in sufficient quantities. Therefore, we will find a way of replacing chondrocytes with fat stem cells to solve the problem of seed cell origin. Previous studies have revealed that transforming growth factor-β ( ...
DOI:
10.1016/j.bbrc.2020.06.147

85. Meniscal tissue engineering via 3D printed PLA monolith with carbohydrate based self-healing interpenetrating network hydrogel

Author:
Santosh Gupta; Akriti Sharma; J. Vasantha Kumar; Vineeta Sharma; Piyush Kumar Gupta; Rama Shanker Verma
Source:
International journal of biological macromolecules 2020 v.162 pp. 1358-1371
ISSN:
0141-8130
Subject:
alginates; biocompatibility; biocompatible materials; bioprinting; calcium; cartilage; chondrocytes; chondrogenesis; collagen; crosslinking; human cell lines; humans; hydrogels; schiff bases; stem cells; three-dimensional printing; tissue engineering; tissue scaffolds; tissue transplantation
Abstract:
... Failure of bioengineered meniscus implant after transplantation is a major concern owing to mechanical failure, lack of chondrogenic capability and patient specific design. In this article, we have, for the first time, fabricated a 3D printed scaffold with carbohydrate based self-healing interpenetrating network (IPN) hydrogels-based monolith construct for load bearing meniscus tissue. 3D printed ...
DOI:
10.1016/j.ijbiomac.2020.07.238

86. MicroRNA-191 regulates differentiation and migration of mesenchymal stem cells and their paracrine effect on angiogenesis

Author:
Yuanxing Liu; Xi Liu; Pengxiang Ye; Xiafei Zhang; Arndt F. Schilling; Tomo Yonezawa; Guifang Gao; Xiaofeng Cui
Source:
Biotechnology letters 2020 v.42 no.9 pp. 1777-1788
ISSN:
0141-5492
Subject:
angiogenesis; biotechnology; bone formation; bone marrow; chondrogenesis; embryogenesis; microRNA
Abstract:
... MicroRNAs (miRNAs) are critical regulators in organ development. Among them, miR-191 is known to be regulated in early embryogenesis and dysregulated in cancer. This role in undifferentiated tissues suggests a possible part of miR-191 also in bone marrow derived mesenchymal stem cells (BMSCs) physiology. Here, we report that miR-191 decreased MMP expression and migration of BMSCs. Conditioned medi ...
DOI:
10.1007/s10529-020-02907-z

87. Microencapsulation improves chondrogenesis in vitro and cartilaginous matrix stability in vivo compared to bulk encapsulation

Author:
Fanyi Li; Clara Levinson; Vinh X. Truong; Lee Ann Laurent-Applegate; Katharina Maniura-Weber; Helmut Thissen; John S. Forsythe; Marcy Zenobi-Wong; Jessica E. Frith
Source:
Biomaterials science 2020 v.8 no.6 pp. 1711-1725
ISSN:
2047-4849
Subject:
cartilage; chondrocytes; chondrogenesis; genes; histology; microencapsulation; microgels; nutrient transport; phenotype; porous media; shear stress; staining; tissue repair
Abstract:
... The encapsulation of cells into microgels is attractive for applications in tissue regeneration. While cells are protected against shear stress during injection, the assembly of microgels after injection into a tissue defect also forms a macroporous scaffold that allows effective nutrient transport throughout the construct. However, in most of current strategies that form microgel-based macroporou ...
DOI:
10.1039/c9bm01524h

88. Noggin, an inhibitor of bone morphogenetic protein signaling, antagonizes TGF-β1 in a mouse model of osteoarthritis

Author:
Liru Wen; Manman Gao; Zhongyuan He; Peng Guo; Zhen Liu; Penghui Zhang; Fu Zhang; Dafu Chen; Guangqian Zhou; Zhiyu Zhou
Source:
Biochemical and biophysical research communications 2021 v.570 pp. 199-205
ISSN:
0006-291X
Subject:
antagonists; bone morphogenetic proteins; cartilage; chondrogenesis; mice; osteoarthritis; therapeutics
Abstract:
... Osteoarthritis (OA) is the most common joint disease worldwide; however, disease-modifying treatments are lacking because of the complicated pathological mechanisms. As a breakthrough, aberrant activation of transforming growth factor-β 1 (TGF-β1)in subchondral bone has been confirmed as an essential pathomechanism for OA progression, and has become a potential therapeutic target. In addition to R ...
DOI:
10.1016/j.bbrc.2021.07.044

89. Parental exposure to bisphenol A affects pharyngeal cartilage development and causes global transcriptomic changes in zebrafish (Danio rerio) offspring

Author:
Wenlong Huang; Shukai Zheng; Jiefeng Xiao; Caixia Liu; Taifeng Du; Kusheng Wu
Source:
Chemosphere 2020 v.249 pp. 126537
ISSN:
0045-6535
Subject:
Danio rerio; adverse effects; animal models; apoptosis; aquatic ecosystems; bisphenol A; cartilage; chondrocytes; chondrogenesis; eggs; endocrine-disrupting chemicals; fish; gene expression regulation; gene regulatory networks; genes; head; heart rate; hyperplasia; mitogen-activated protein kinase; oxidative stress; pharynx; progeny; reactive oxygen species; sequence analysis; signal transduction; toxicity; transcriptomics
Abstract:
... As one of the most common endocrine-disrupting chemicals (EDCs), bisphenol A (BPA) is a threat to aquatic ecosystems. Despite a rich literature addressing the adverse effects of BPA on various systems in fish models, the potential impact of parental BPA exposure on offspring pharyngeal cartilage development is poorly understood.Adult zebrafish (F0) were exposed to BPA (1.0 μM) or control for 7 day ...
DOI:
10.1016/j.chemosphere.2020.126537

90. Preparation and characterization of an injectable dexamethasone-cyclodextrin complexes-loaded gellan gum hydrogel for cartilage tissue engineering

Author:
Joo Hee Choi; Ain Park; Wonchan Lee; Jina Youn; Min A. Rim; Wooyoup Kim; Namyeong Kim; Jeong Eun Song; Gilson Khang
Source:
Journal of controlled release 2020 v.327 pp. 747-765
ISSN:
0168-3659
Subject:
DNA; Fourier transform infrared spectroscopy; animal models; cartilage; chemical structure; chondrogenesis; dexamethasone; drug delivery systems; gelatinization temperature; gellan gum; gene expression; genes; glycosaminoglycans; histology; hydrogels; hydrophobicity; in vitro studies; in vivo studies; inflammation; mechanical properties; medicine; nuclear magnetic resonance spectroscopy; rabbits; rats; staining; tissue engineering; toxicity testing; viscosity; weight loss
Abstract:
... In this study, 6-(6-aminohexyl) amino-6-deoxy-β-cyclodextrin-gellan gum complex hydrogel (HCD-GG) was developed to enhance the affinity of anti-inflammatory drug dexamethasone (Dx), improve chondrogenesis, and decrease the inflammatory response. The modified chemical structure was confirmed by NMR and FTIR. Mechanical and physicochemical properties were characterized by performing viscosity study, ...
DOI:
10.1016/j.jconrel.2020.08.049

91. Protective effects of Phlomis umbrosa extract on a monosodium iodoacetate–induced osteoarthritis model and prediction of molecular mechanisms using transcriptomics

Author:
Jin Mi Chun; A Yeong Lee; Jae Yong Nam; Min Young Lee; Mu Seog Choe; Kyung Seob Lim; Chul Kim; Joong-Sun Kim
Source:
Phytomedicine 2021 v.81 pp. 153429
ISSN:
0944-7113
Subject:
Phlomis; RNA; cartilage; chondrogenesis; collagen; computed tomography; disease progression; gene expression; genomics; histopathology; models; osteoarthritis; prediction; rheumatoid arthritis; sequence analysis; signal transduction; therapeutics; transcriptomics
Abstract:
... Phlomis umbrosa Turczaninow root has been traditionally used to treat fractures, rheumatoid arthritis, and arthralgia. However, the effects and mechanisms of P. umbrosa on osteoarthritis (OA) remain poorly understood and a functional genomic approach has not been investigated.The purpose of this study was to investigate the effects and mechanisms of P. umbrosa extract (PUE) on OA using transcripto ...
DOI:
10.1016/j.phymed.2020.153429

92. Role of sox family genes in teleostean reproduction-an overview

Author:
Arumugam Anitha; Balasubramanian Senthilkumaran
Source:
Reproduction and breeding 2021 v.1 no.1 pp. 22-31
ISSN:
2667-0712
Subject:
animal growth; cell differentiation; chondrogenesis; embryogenesis; fish; hematopoiesis; neural crest; neurogenesis; oligodendroglia; sex determination; testes
Abstract:
... SRY-box (SOX) family genes are mostly transcription factors essential for animal growth, involved in developing oligodendrocyte, neural crest, lens, and testis. SOX proteins are functionally implicated in sex determination/differentiation, early embryogenesis, chondrogenesis, hemopoiesis, neurogenesis, cellular differentiation, and in maintaining multipotency. They function uniquely in different c ...
DOI:
10.1016/j.repbre.2021.02.004

93. Solubilized Cartilage ECM Facilitates the Recruitment and Chondrogenesis of Endogenous BMSCs in Collagen Scaffolds for Enhancing Microfracture Treatment

Author:
Yan Lu; Yuxiang Wang; Hanjie Zhang; Zizhao Tang; Xiaolin Cui; Xing Li; Jie Liang; Qiguang Wang; Yujiang Fan; Xingdong Zhang
Source:
ACS applied materials & interfaces 2021 v.13 no.21 pp. 24553-24564
ISSN:
1944-8252
Subject:
biocompatible materials; biological assessment; bone marrow; cartilage; chondrogenesis; collagen; extracellular matrix; micro-computed tomography; solubilization; swine
Abstract:
... Articular cartilage has very poor intrinsic healing ability and its repair remains a significant clinical challenge. To promote neocartilage regeneration, we fabricated two collagen (Col) scaffolds functionalized with a porcine decellularized extracellular matrix (dECM) in the forms of particle and solution named pE-Col and sE-Col, respectively. Their differences were systematically compared, incl ...
DOI:
10.1021/acsami.1c07530

94. Sustained release of TGF-β3 from polysaccharide nanoparticles induces chondrogenic differentiation of human mesenchymal stromal cells

Author:
Menzel H.; Yvonne Roger; Steffen Sydow; Laura Burmeister; Henning Menzel; Andrea Hoffmann
Source:
Colloids and surfaces 2020 v.189 pp. 110843
ISSN:
0927-7765
Subject:
antibiotics; bioactive compounds; chondrogenesis; drugs; humans; medical treatment; mesenchymal stromal cells; nanoparticles; patients; polysaccharides; transforming growth factor beta 3
Abstract:
... Medical treatment of certain diseases and biomedical implants are tending to use delivery systems on the nanoscale basis for biologically active factors including drugs (e. g. antibiotics) or growth factors. Nanoparticles are a useful tool to deliver bioactive substances of different chemical nature directly to the site where it is required in the patient. Here we developed three innovative delive ...
DOI:
10.1016/j.colsurfb.2020.110843

95. Synergistic interaction of hTGF-β3 with hBMP-6 promotes articular cartilage formation in chitosan scaffolds with hADSCs: implications for regenerative medicine

Author:
Yijiang Huang; Daniel Seitz; Yan Chevalier; Peter E. Müller; Volkmar Jansson; Roland M. Klar
Source:
BMC biotechnology 2020 v.20 no.1 pp. 48
ISSN:
1472-6750
Subject:
biotechnology; bone formation; cartilage; chitosan; chondrogenesis; employment; gene expression; gene expression regulation; genes; growth factors; humans; hypertrophy; medicine; quantitative polymerase chain reaction; stem cells; synergism; transcription (genetics); viability
Abstract:
... BACKGROUND: Human TGF-β₃ has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media used for the assessment of chondrogenesis associated with various stem cell types on carrier matrices. However, in vivo data suggests that TGF-β₃ and its other isoforms also in ...
DOI:
10.1186/s12896-020-00641-y

96. Three dimensional printed degradable and conductive polymer scaffolds promote chondrogenic differentiation of chondroprogenitor cells

Author:
Aruna Prasopthum; Zexing Deng; Ilyas M. Khan; Zhanhai Yin; Baolin Guo; Jing Yang
Source:
Biomaterials science 2020 v.8 no.15 pp. 4287-4298
ISSN:
2047-4849
Subject:
absorption; adsorption; biocompatible materials; biodegradability; biosensors; brittleness; cartilage; chondrogenesis; composite polymers; fibronectins; medicine; muscles; solubility; three-dimensional printing; tissue repair
Abstract:
... Conductive polymers have been used for various biomedical applications including biosensors, tissue engineering and regenerative medicine. However, the poor processability and brittleness of these polymers hinder the fabrication of three-dimensional structures with desirable geometries. Moreover, their application in tissue engineering and regenerative medicine has been so far limited to excitable ...
DOI:
10.1039/d0bm00621a

97. An immortalized human adipose-derived stem cell line with highly enhanced chondrogenic properties

Author:
Dakota B. Katz; Nguyen P.T. Huynh; Alireza Savadipour; Ilan Palte; Farshid Guilak
Source:
Biochemical and biophysical research communications 2020 v.530 no.1 pp. 252-258
ISSN:
0006-291X
Subject:
cartilage; chondrocytes; chondrogenesis; collagen; drugs; homeostasis; humans; research
Abstract:
... Human adipose-derived stem cells (ASCs) are a commonly used cell type for cartilage tissue engineering. However, donor-to-donor variability, cell heterogeneity, inconsistent chondrogenic potential, and limited expansion potential can hinder the use of these cells for modeling chondrogenesis, in vitro screening of drugs and treatments for joint diseases, or translational applications for tissue eng ...
DOI:
10.1016/j.bbrc.2020.07.016

98. An injectable hydrogel having proteoglycan-like hierarchical structure supports chondrocytes delivery and chondrogenesis

Author:
Jijo Thomas; Vianni Chopra; Anjana Sharma; Vineeta Panwar; Swati Kaushik; Swati Rajput; Monika Mittal; Rajdeep Guha; Naibedya Chattopadhyay; Deepa Ghosh
Source:
International journal of biological macromolecules 2021 v.190 pp. 474-486
ISSN:
0141-8130
Subject:
biocompatibility; biodegradability; carboxymethylcellulose; cartilage; chitosan; chondrocytes; chondrogenesis; chondroitin sulfate; collagen; crosslinking; humans; hyaluronic acid; hydrogels; proteoglycans; viability
Abstract:
... The ECM of cartilage is composed of proteoglycans (PG) that contain glycosaminoglycan (GAG), aggrecan, hyaluronic acid (HA) and other molecular components which play an important role in regulating chondrocyte functions via cell-matrix interactions, integrin-mediated signalling etc. Implantation of chondrocytes encapsulated in scaffolds that mimic the micro-architecture of proteoglycan, is expecte ...
DOI:
10.1016/j.ijbiomac.2021.08.226

99. The involvement of cytokine-like 1 (Cytl1) in chondrogenesis and cartilage metabolism

Author:
Weicong Zhu; Xiaohong Yang; Shaojie Liu; Min Wang; Suihui Ye; Huixing Luo; Shuliang Cui
Source:
Biochemical and biophysical research communications 2020 v.529 no.3 pp. 608-614
ISSN:
0006-291X
Subject:
adults; bone marrow; cartilage; chondrocytes; chondrogenesis; gene expression; homeostasis; metabolism; phenotype; research; skeletal development; skeleton
Abstract:
... The Cytokine-like 1 (Cytl1) is first identified in bone marrow cells and preferentially expressed in cartilaginous tissue, and showed chondrogenic effects in mesenchymal cells, not essential for cartilage or bone development as in Cytl1 knock-out mice but associated with cartilage inflammatory and destruction. Here, we show the involvement of Cytl1 in chondrogenesis. Using specified chondrogenic e ...
DOI:
10.1016/j.bbrc.2020.06.069

100. miR-23a-3p regulated by LncRNA SNHG5 suppresses the chondrogenic differentiation of human adipose-derived stem cells via targeting SOX6/SOX5

Author:
Zhen Yang; Zhijing Ren; Rongfeng She; Jun Ao; Qingde Wa; Zeyu Sun; Bo Li; Xiaobin Tian
Source:
Cell and tissue research 2021 v.383 no.2 pp. 723-733
ISSN:
0302-766X
Subject:
Western blotting; cartilage; chondrogenesis; humans; microRNA; quantitative polymerase chain reaction; research
Abstract:
... Cartilage generation and degradation are controlled by miRNAs. Our previous study showed miR-23a-3p was downregulated during chondrogenic differentiation in chondrogenic human adipose-derived mesenchymal stem cells (hADSCs). In the present study, we explored the function of miR-23a-3p in chondrogenesis differentiation. The role of miR-23a-3p in chondrogenic differentiation potential of hADSCs was ...
DOI:
10.1007/s00441-020-03289-4