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1. Calcium pyruvate attenuates fat deposition by augmenting fatty acid oxidation and inhibiting glucose oxidation in juvenile large yellow croaker (Larimichthys crocea) consuming a high-fat diet

Author:
Yunqiang Zhang; Wencong Lai; Qiuchi Chen; Wei Fang; Kun Cui; Yuliang He; Xueshan Li; Kangsen Mai; Qinghui Ai
Source:
Aquaculture 2023 v.562 pp. 738778
ISSN:
0044-8486
Subject:
AMP-activated protein kinase; Larimichthys crocea; Western blotting; acetyl-CoA carboxylase; alanine transaminase; antioxidant activity; aquaculture; aspartate transaminase; beta oxidation; blood glucose; calcium; carnitine palmitoyltransferase; catalase; citrates; diacylglycerol acyltransferase; dietary supplements; droplet size; fatty acid transport proteins; fatty-acid synthase; fish; gene expression; glucokinase; glucose; growth performance; hepatosomatic index; hexokinase; high density lipoprotein cholesterol; high fat diet; interleukin-1beta; juveniles; lipemic effect; lipogenesis; liver; low density lipoprotein cholesterol; malate dehydrogenase; muscles; obesity; oxidation; phosphofructokinases; prostaglandin synthase; protein synthesis; pyruvate dehydrogenase (lipoamide); pyruvate kinase; pyruvic acid; succinate dehydrogenase (quinone); triacylglycerol lipase; triacylglycerols; weight gain
Abstract:
... Calcium pyruvate (CP), known as an energetic aid, has been shown to enhance lipid utilization and prevent obesity in mammals. However, very few studies were conducted to evaluate the potential implications of CP for aquatic animals. This study examined the role of CP on fat deposition in large yellow croaker by feeding fish with high-fat diets containing four levels of dietary CP (0, 0.375%, 0.75% ...
DOI:
10.1016/j.aquaculture.2022.738778

2. Determination of metallic nanoparticles in air filters by means single particle inductively coupled plasma mass spectrometry

Author:
Daniel Torregrosa; Guillermo Grindlay; Miguel de la Guardia; Luis Gras; Juan Mora
Source:
Talanta 2023 v.252 pp. 123818
ISSN:
0039-9140
Subject:
air; ammonium hydroxide; atomic absorption spectrometry; calibration; citrates; citric acid; glass; glass fibers; microwave ovens; microwave treatment; nanogold; platinum; potassium nitrate; sodium nitrate; thiourea; ultrasonic treatment
Abstract:
... Single particle inductively coupled plasma mass spectrometry (spICP-MS) has been explored for the determination of metallic nanoparticles (NPs) in air. Different extraction strategies (i.e., direct immersion, hard cap espresso, ultrasound-assisted and microwave-assisted extraction) and extracting solvents (i.e., citric acid, trisodium citrate, potassium nitrate, sodium nitrate, thiourea, disodium ...
DOI:
10.1016/j.talanta.2022.123818

3. Identification of co-expressed networks and key genes associated with organic acid in peach fruit

Author:
Qi Wang; Ke Cao; Yong Li; Jinlong Wu; Jiaqi Fan; Tiyu Ding; Irshad Ahmad Khan; Lirong Wang
Source:
Scientia horticulturae 2023 v.307 pp. 111496
ISSN:
0304-4238
Subject:
citrates; fruiting; genes; malates; peaches; phenotype; transcriptomics
Abstract:
... Malate, citrate, and quinate are the major organic acids in ripen peach fruit, the content of which undergoes considerable variations during fruit development and maturation. The molecular mechanisms underlying these variations remain largely unclear. In this study, weighted gene co-expression network analysis (WGCNA) of two accessions in five developmental stages was performed to identify network ...
DOI:
10.1016/j.scienta.2022.111496

4. Improving sludge dewaterability via Fe2+ chelated citrate activated peroxydisulfate oxidation

Author:
Donghai Yuan; Xinyu Li; Siyu Xiong; Jun Cui; Jijiao Zhou; Yingying Kou
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 223-233
ISSN:
1001-0742
Subject:
chelating agents; citrates; dewatering; iron; oxidation; particle size; polymers; polysaccharides; sludge; water content; China
Abstract:
... Citrate (Ct) was chosen as a typical chelator used in the Fe²⁺−peroxydisulfate (PDS) process to improve sludge dewaterability. The PDS−Fe²⁺−Ct process exhibited better performance in sludge dewatering than PDS−Fe²⁺. Specifically, with a PDS dosage of 1.2mmol/g VS, the molar ratio of PDS/Fe²⁺ and Ct/Fe²⁺ were 4:5 and 1:4, respectively, the capillary suction time decreased from 155.8 to 24.8sec, and ...
DOI:
10.1016/j.jes.2021.11.022

5. Nitrogen-doped nanocarbon derived from candle soot for persulfate activation on sulfamethoxazole removal: Performance and mechanism

Author:
Danlian Huang; Li Du; Min Cheng; Lingshi Yin; Ruihao Xiao; Sha Chen; Lei Lei; Yashi Chen; Guangfu Wang; Weihua Xu; Yunguo Liu
Source:
Journal of colloid and interface science 2023 v.629 pp. 685-696
ISSN:
0021-9797
Subject:
ammonium; carbon; catalytic activity; citrates; electron paramagnetic resonance spectroscopy; electron transfer; pH; soot; sulfamethoxazole; wastewater treatment
Abstract:
... Recently, carbon materials have attracted much attention in activating persulfate (PS) for the removal of organic pollutants. Seeking a greener, lower-cost, and higher-performance carbon material has become an important aspect of research. In this study, candle soot was innovatively used as a nanocarbon material, and its performance for PS activation was improved by simple ammonium ferric citrate ...
DOI:
10.1016/j.jcis.2022.08.121

6. Renewable biohydrogen production from Clostridium sp. LQ25 using different forms of ferric as electron acceptor

Author:
Shan Zhang; Xiaodan Zhang; Yuan Yuan; Kaiqiang Li; Hongyan Liu
Source:
Science of the total environment 2023 v.855 pp. 158911
ISSN:
0048-9697
Subject:
Clostridium; butyrates; citrates; environment; fermentation; ferric hydroxide; gene expression regulation; glucose; hydrogen production
Abstract:
... Clostridium sp. LQ25 was cultured in different forms of ferric (ferric citrate and ferric hydroxide) as electron acceptors to investigate growth, ferric reduction, hydrogen production, fermentation products and fermentation process. The growth of the strain LQ25 detected by protein was 82.8 ± 2.1 mg/L and 73.5 ± 1.7 mg/L using ferric citrate and ferric hydroxide as electron acceptors, which was 33 ...
DOI:
10.1016/j.scitotenv.2022.158911

7. The importance of selecting crystal form for triazole fungicide tebuconazole to enhance its botryticidal activity

Author:
Yongjun Chen; Dongmei Yan; Jialin Xu; Hui Xiong; Shaorong Luan; Ciying Xiao; Qingchun Huang
Source:
Science of the total environment 2023 v.854 pp. 158778
ISSN:
0048-9697
Subject:
Botrytis cinerea; X-ray diffraction; antifungal properties; citrates; crystal structure; environment; metabolism; metabolites; mutation; oxaloacetic acid; phosphates; sterols; structure-activity relationships; tebuconazole; triazoles; tricarboxylic acid cycle
Abstract:
... The growing evidences of resistant fungi stimulate fully understanding tebuconazole regarding its crystal structure on fungicidal activity. In this study, the crystal structures of six technical tebuconazoles (BX, HH, JP, QZ, SJ, and YT) were characterized by using high-resolution X-ray powder diffraction and three-dimensional crystal structure modeling. A structure-activity relationship of the te ...
DOI:
10.1016/j.scitotenv.2022.158778

8. 1H NMR-Based Metabolomics Reveals the Intrinsic Interaction of Age, Plasma Signature Metabolites, and Nutrient Intake in the Longevity Population in Guangxi, China

Author:
He Li; Minhong Ren; Quanyang Li
Source:
Nutrients 2022 v.14 no.12 pp. -
ISSN:
2072-6643
Subject:
alanine; aspartic acid; biomarkers; biosynthesis; carnitine; choline; citrates; dietary fiber; elderly; energy; fat intake; food composition; gluconeogenesis; glutamic acid; glycolysis; glycoproteins; lactic acid; longevity; metabolites; metabolomics; nuclear magnetic resonance spectroscopy; phenylalanine; trimethylamine; tryptophan; tyrosine; valine; China
Abstract:
... Health and longevity populations have distinct metabolic and nutrient intake profiles. However, the relationship between biomarkers of longevity-related metabolites and dietary nutrient intake profiles, as well as metabolic markers associated with longevity features, have not been fully elucidated. Therefore, ¹H nuclear magnetic resonance (¹H NMR)-based plasma metabolomics profiling was conducted ...
DOI:
10.3390/nu14122539

9. 1α,25-dihydroxyvitamin D reduction of MCF10A-ras cell viability in extracellular matrix detached conditions is dependent on regulation of pyruvate carboxylase

Author:
Madeline P. Sheeley; Violet A. Kiesel; Chaylen Andolino; Nadia A. Lanman; Shawn S. Donkin; Stephen D. Hursting; Michael K. Wendt; Dorothy Teegarden
Source:
Journal of nutritional biochemistry 2022 v.109 pp. 109116
ISSN:
0955-2863
Subject:
aspartic acid; breast neoplasms; cell viability; citrates; extracellular matrix; glutamic acid; glutamine; malates; metabolites; metastasis; neoplasm cells; neoplasm progression; protein synthesis; pyruvate carboxylase
Abstract:
... An emerging hallmark of cancer is cellular metabolic reprogramming to adapt to varying cellular environments. Throughout the process of metastasis cancer cells gain anchorage independence which confers survival characteristics when detached from the extracellular matrix (ECM). Previous work demonstrates that the bioactive metabolite of vitamin D, 1α,25-dihydroxyvitamin D (1,25[OH]₂D), suppresses c ...
DOI:
10.1016/j.jnutbio.2022.109116

10. 2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea

Author:
Guangjin Li; Yong Chen; Zhanquan Zhang; Boqiang Li; Tong Chen; Shiping Tian
Source:
Postharvest biology and technology 2022 v.193 pp. 112057
ISSN:
0925-5214
Subject:
Botrytis cinerea; arginine; autophagy; biogenesis; biosynthesis; citrates; conidia; disease control; disease prevalence; fruits; fungal growth; fungi; gene expression; germ tube; germination; gray mold; pathogenicity; postharvest diseases; proteolysis; ribosomes; technology; transcriptomics; volatile organic compounds
Abstract:
... The volatile organic compounds produced by plants and microorganisms have been widely used for postharvest disease control due to their high antifungal capacity. In this research, a volatile product of citrate metabolism, 2,3-butanedione, was assayed against gray mold caused by Botrytis cinerea on postharvest fruit. 2,3-Butanedione treatment exhibited a distinct inhibitory effect on colony growth, ...
DOI:
10.1016/j.postharvbio.2022.112057

11. 5‐Aminolevulinic acid and sodium ferrous citrate ameliorate muscle aging and extend healthspan in Drosophila

Author:
Naoko Nozawa; Marie Noguchi; Kanako Shinno; Maki Tajima; Shingo Aizawa; Taro Saito; Akiko Asada; Takuya Ishii; Masahiro Ishizuka; Koichi M. Iijima; Kanae Ando
Source:
FEBS Open Bio 2022 v.12 no.1 pp. 295-305
ISSN:
2211-5463
Subject:
Drosophila melanogaster; adults; aminolevulinic acid; citrates; corn meal; dietary supplements; fruits; humans; longevity; membrane potential; mitochondria; mitochondrial membrane; muscles; phosphates; sodium
Abstract:
... Declines in mitochondrial functions are associated with aging. The combination of 5‐aminolevulinic acid (5‐ALA) and sodium ferrous citrate (SFC) improves mitochondrial functions in cultured cells. In this study, we investigated the effects of dietary supplementation with 5‐ALA and SFC (5‐ALA/SFC) on the healthspan and life span of Drosophila melanogaster. Adult Drosophila fruit flies were fed corn ...
DOI:
10.1002/2211-5463.13338

12. Acid complexation of iron controls the fate of hydrogen peroxide in model wine

Author:
Thi H. Nguyen; Andrew L. Waterhouse
Source:
Food chemistry 2022 v.377 pp. 131910
ISSN:
0308-8146
Subject:
acetaldehyde; acidity; citrates; ethanol; food chemistry; hydrogen peroxide; iron; malates; oxidation; pH; stoichiometry; sulfur dioxide; wines
Abstract:
... At a key branchpoint in wine oxidation, hydrogen peroxide reacts either with iron(II), leading to the Fenton oxidation of ethanol, or with sulfur dioxide, precluding oxidation. The fate of H₂O₂ was investigated in anoxic model wines with different pH and acid buffers. In the absence of SO₂, anoxic conditions allowed the stoichiometric production of acetaldehyde from H₂O₂ despite iron(II) being lim ...
DOI:
10.1016/j.foodchem.2021.131910

13. Addition of iron to agricultural topsoil and subsoil is not an effective C sequestration strategy

Author:
Erik S. Button; David R. Chadwick; David L. Jones
Source:
Geoderma 2022 v.409 pp. 115646
ISSN:
0016-7061
Subject:
arable soils; carbon; carbon dioxide; carbon sequestration; carbonates; citrates; glucose; iron; microbial biomass; mineralization; pH; soil acidification; soil organic matter; soil solution; subsoil; topsoil
Abstract:
... The interaction of soil organic matter (SOM) with Fe-containing minerals represents a key mechanism that promotes carbon (C) stabilisation in soil. The addition of Fe-rich industrial by-products to soil may therefore help accelerate C storage. Our understanding of the effects of exogenous Fe addition (Fe (oxy)hydroxide vs. Fe chloride) on SOM dynamics and C dynamics in agricultural soils, especial ...
DOI:
10.1016/j.geoderma.2021.115646

14. Al2O3 anchored silver and gold nanoparticles as accessible, stable, and re-usable catalysts

Author:
Graham Beaton; Joshua Zacks; Kevin Stamplecoskie
Source:
Colloids and surfaces 2022 v.646 pp. 128972
ISSN:
0927-7757
Subject:
aluminum oxide; catalysts; citrates; gold; nanogold; nanosilver; p-nitrophenol; silver
Abstract:
... Nanoparticles of silver and gold have become widely researched as homogeneous catalysts for organic transformations. Silver systems have shown promising activity but are often hindered by their instability and difficulty in recycling. In this work we present nanoparticles of silver and gold, colloidally stabilized on aluminum oxide, and their performance as re-useable catalysts for the reduction o ...
DOI:
10.1016/j.colsurfa.2022.128972

15. Allosteric transitions of rabbit skeletal muscle lactate dehydrogenase induced by pH-dependent dissociation of the tetrameric enzyme

Author:
Luca Giacinto Iacovino; Martina Rossi; Giuseppina Di Stefano; Valentina Rossi; Claudia Binda; Maurizio Brigotti; Fabio Tomaselli; Alberto Pietro Pasti; Fabrizio Dal Piaz; Stefano Cerini; Alejandro Hochkoeppler
Source:
Biochimie 2022 v.199 pp. 23-35
ISSN:
0300-9084
Subject:
acidosis; citrates; dissociation; enzyme kinetics; lactate dehydrogenase; lactic acid; oxygen; pH; pyruvic acid; rabbits; secretion; skeletal muscle
Abstract:
... Among the functions exerted by eukaryotic lactate dehydrogenases, it is of importance the generation of lactate in muscles subjected to fatigue or to limited oxygen availability, with both these conditions triggering a decrease of cellular pH. However, the mutual dependence between lactate dehydrogenase (LDH) catalytic action and lactic acidosis is far from being fully understood. Here we show tha ...
DOI:
10.1016/j.biochi.2022.03.008

16. Alverine citrate promotes myogenic differentiation and ameliorates muscle atrophy

Author:
Jong Hyeon Yoon; Seung-Min Lee; Younglang Lee; Min Ju Kim; Jae Won Yang; Jeong Yi Choi; Ju Yeon Kwak; Kwang-Pyo Lee; Yong Ryoul Yang; Ki-Sun Kwon
Source:
Biochemical and biophysical research communications 2022 v.586 pp. 157-162
ISSN:
0006-291X
Subject:
citrates; dexamethasone; intestines; medicine; muscle development; muscle tissues; muscles; myoblasts; myotubes; oral administration; physical activity; research; sarcopenia; stomach
Abstract:
... Sarcopenia is the age-related loss of muscle mass and function and no pharmacological medication has been approved for its treatment. We established an atrogin-1/MAFbx promoter assay to find drug candidates that inhibit myotube atrophy. Alverine citrate (AC) was identified using high-throughput screening of an existing drug library. AC is an established medicine for stomach and intestinal spasms. ...
DOI:
10.1016/j.bbrc.2021.11.076

17. Analysis of HMF and furfural in hydrolyzed lignocellulosic biomass by HPLC-DAD-based method using FDCA as internal standard

Author:
Cristian Acker Godoy; Patrícia Valderrama; Andreia Cristina Furtado; Marcela Boroski
Source:
MethodsX 2022 v.9 pp. 101774
ISSN:
2215-0161
Subject:
acetonitrile; acidity; biomass; chemical species; citrates; coproducts; deprotonation; diodes; fructose; furfural; glucose; high performance liquid chromatography; hydrolysis; hydroxymethylfurfural; lignocellulose; pH; sugarcane bagasse
Abstract:
... HMF (hydroxymethylfurfural), a compound that occurs naturally in food, is derived from the dehydration of monosaccharides (glucose and fructose) in products subjected to high-temperature treatments or to aging processes. HMF can be obtained by acid-catalyzed dehydration of lignocellulosic biomasses such as sugarcane bagasse and other agricultural residues. In this work, analytical quantification o ...
DOI:
10.1016/j.mex.2022.101774

18. Analysis of multiple pesticide residues in market samples of okra and associated dietary risk assessment for consumers

Author:
Syeda Nazish Ali; Nazia Rafique; Shazia Akhtar; Touqeer Taj; Farrakh Mehboob
Source:
Environmental science and pollution research 2022 v.29 no.31 pp. 47561-47570
ISSN:
0944-1344
Subject:
European Union; acetonitrile; acute exposure; bifenthrin; body weight; carbofuran; chronic exposure; citrates; human health; laboratory techniques; markets; nutrition risk assessment; okra; pollution; research; risk; secondary amines; thiamethoxam; triazophos; Pakistan
Abstract:
... European Union Reference Laboratory method for Fruits and Vegetables (EURL-FV-2010-M1) for the quantification of pesticide residues was verified for the determination of multiple pesticide residues in okra. The targeted pesticides were extracted using acetonitrile with citrate buffer salts followed by cleanup with primary secondary amine (PSA) and analyzed on LC–MS/MS. The recoveries for all the t ...
DOI:
10.1007/s11356-022-19197-9

19. Antifungal activity of photo-biosynthesized silver nanoparticles (AgNPs) from organic constituents in orange peel extract against phytopathogenic Macrophomina phaseolina

Author:
Yasser Mahmoud A. Mohamed; Ibrahim E. Elshahawy
Source:
European journal of plant pathology 2022 v.162 no.3 pp. 725-738
ISSN:
0929-1873
Subject:
Citrus sinensis; Macrophomina phaseolina; antifungal properties; charcoal rot; citrates; damping off; faba beans; greenhouse experimentation; greenhouses; hesperidin; nanosilver; orange peels; plant pathology; soil; soil fungi; spectroscopy; transmission electron microscopy; virulence; Egypt
Abstract:
... The soil-borne fungus Macrophomina phaseolina causes charcoal rot, which is one of the most damaging threats to the faba bean in Egypt. The goal of this work was to investigate the inhibitory effects of silver nanoparticles AgNPs on Modiolula phaseolina in vitro, as well as their effectiveness in reducing the incidence of charcoal rot in greenhouse settings. AgNPs were synthesized using a chemical ...
DOI:
10.1007/s10658-021-02434-1

20. Application of Design of Experiments to the Analysis of Fruit Juice Deacidification Using Electrodialysis with Monopolar Membranes

Author:
Marcello Fidaleo; Giordana Ventriglia
Source:
Foods 2022 v.11 no.12 pp. -
ISSN:
2304-8158
Subject:
citrates; citric acid; deacidification; electric current; electrodialysis; fruits; pH; pineapple juice; titratable acidity
Abstract:
... Despite the beneficial health effects of fruit juices, the high content of organic acids and low pH of some of them limit their consumption. The aim of this work was to study the deacidification of fruit juices using electrodialysis (ED) with monopolar membranes. Aqueous solutions of citric acid were used in ED deacidification experiments following a factorial design with citric acid concentration ...
DOI:
10.3390/foods11121770

21. Assessing and predicting phosphorus phytoavailability from sludge incineration ashes

Author:
Claude-Alla Joseph; Lotfi Khiari; Jacques Gallichand; Ned Beecher
Source:
Chemosphere 2022 v.288 pp. 132498
ISSN:
0045-6535
Subject:
Lolium; ammonium; bioavailability; citrates; clay; clay soils; greenhouse experimentation; organic fertilizers; oxalates; phosphorus; phosphorus fertilizers; phytoaccumulation; rock phosphate; sandy loam soils; sludge; solubility; triple superphosphate; Canada
Abstract:
... Incineration of municipal sludge and agri-food by-products generates large quantities of ash that can be used in agriculture as phosphorus fertilizer. The fertilizing potential of sludge incineration ash (SIA) from 12 cities in Canada and the United States was tested in a greenhouse experiment against a synthetic fertilizer (TSP: triple superphosphate), a natural fertilizer (RP: rock phosphate), a ...
DOI:
10.1016/j.chemosphere.2021.132498

22. Asymmetric and Symmetric Redox Flow Batteries for Energy-Efficient, High-Recovery Water Desalination

Author:
Gowri Mohandass; Weikun Chen; Sitaraman Krishnan; Taeyoung Kim
Source:
Environmental science & technology 2022 v.56 no.7 pp. 4477-4488
ISSN:
1520-5851
Subject:
brackish water; citrates; cyanides; desalination; electric potential difference; electrochemistry; energy efficiency; environmental science; freshwater; infrastructure; power generation; solar energy
Abstract:
... Electrochemical separation offers an energy-efficient means to desalinate brackish water, a relatively untapped but increasingly utilized water source for freshwater supply. Several electrochemical techniques are being developed to enable low-energy desalination combined with energy storage. We report a new approach that produced a peak power density of 6.0 mW cm–² from the energy stored in iron c ...
DOI:
10.1021/acs.est.1c08609

23. Binding mechanism and binding free energy of amino acids and citrate to hydroxyapatite surfaces as a function of crystallographic facet, pH, and electrolytes

Author:
Samuel E. Hoff; Juan Liu; Hendrik Heinz
Source:
Journal of colloid and interface science 2022 v.605 pp. 685-700
ISSN:
0021-9797
Subject:
Gibbs free energy; calcium; citrates; drugs; hydroxyapatite; mineralization; molecular dynamics; pH; peptides; protonation; uncertainty
Abstract:
... Hydroxyapatite (HAP) is the major mineral phase in bone and teeth. The interaction of individual amino acids and citrate ions with different crystallographic HAP surfaces has remained uncertain for decades, creating a knowledge gap to rationally design interactions with peptides, proteins, and drugs. In this contribution, we quantify the binding mechanisms and binding free energies of the 20 end-c ...
DOI:
10.1016/j.jcis.2021.07.109

24. Biochemical and molecular effects of a commercial diuretic with herbal extract on experimentally induced urolithiasis in chickens

Author:
Mahmoud G. El Sebaei; Reham A. El-Shafei; Mohamed A. El-Adl; Amany Farag; Abdelfattah H. Eladl
Source:
Veterinary research communications 2022 v.46 no.2 pp. 419-430
ISSN:
0165-7380
Subject:
ammonium chloride; antioxidants; ascorbic acid; biomarkers; biotin; calcium; chemical species; citrates; crude protein; diet; histopathology; interferon-gamma; interleukin-6; kidneys; lipid peroxidation; mortality; potassium; renal function; sodium chloride; spleen; toxicity; toxicity testing; urolithiasis; veterinary medicine
Abstract:
... This study evaluated the diuretic, antioxidant, anti-inflammatory, and immunological effects of a commercial diuretic (CD) (composed of ammonium chloride, potassium citrate, sodium chloride, ascorbic acid, biotin, halfa bar extract, and hexamine) on chickens with induced urolithiasis. A total of 100 one-day-old white Hy-Line chicks were fed a basal diet containing 20% crude protein (CP) and 1% Ca ...
DOI:
10.1007/s11259-021-09866-4

25. Biodetoxification mercury by using a marine bacterium Marinomonas sp. RS3 and its merA gene expression under mercury stress

Author:
Mysoon M. Al-Ansari
Source:
Environmental research 2022 v.205 pp. 112452
ISSN:
0013-9351
Subject:
Marinomonas; acetates; aquatic bacteria; carbon; citrates; ecosystems; experimental design; fructose; gene expression; glucose; growth curves; human health; maltose; mercury; mercury(II) reductase; models; pollution; remediation; research; seawater; Red Sea; Saudi Arabia
Abstract:
... Mercury (Hg) pollution in water has been a problem for the ecosystem and human health, thus eco-friendly remediation methods are gaining traction around the world. In this study, a bacterial strain designated as RS3 isolated from the Red Sea (Saudi Arabia) has shown tolerance to more than 250 mg/L of Hg²⁺ on minimum inhibitory studies. The isolate RS3 was identified as Marinomonas sp., (Accession ...
DOI:
10.1016/j.envres.2021.112452

26. Bioremediation of trichloroethylene‐polluted groundwater using emulsified castor oil for slow carbon release and acidification control

Author:
Wei‐Ting Chen; Ku‐Fan Chen; Rao Y. Surmpalli; Tian C. Zhang; Jiun‐Hau Ou; Chih‐Ming Kao
Source:
Water environment research 2022 v.94 no.1 pp. e1673
ISSN:
1061-4303
Subject:
acidification; adsorption; biodegradability; biodegradation; bioremediation; carbon; castor oil; citrates; dechlorination; emulsifying; emulsions; environment; groundwater; lecithins; pH; quantitative polymerase chain reaction; research; sodium phosphate; trichloroethylene; vinyl chloride; viscosity; zeta potential
Abstract:
... In this study, the emulsified castor oil (ECO) substrate was developed for a long‐term supplement of biodegradable carbon with pH buffering capacity to anaerobically bioremediate trichloroethylene (TCE)‐polluted groundwater. The ECO was produced by mixing castor oil, surfactants (sapindales and soya lecithin [SL]), vitamin complex, and a citrate/sodium phosphate dibasic buffer system together for ...
DOI:
10.1002/wer.1673

27. Breast filariasis presenting fibroadenoma like nodules: a rare diagnosis

Author:
J. Priyadharisini; Ashish Ranjan Singh; Prabhat Kumar
Source:
Journal of parasitic diseases 2022 v.46 no.2 pp. 334-336
ISSN:
0971-7196
Subject:
Wuchereria bancrofti; blood; breasts; cell biology; citrates; diethylcarbamazine; females; filariasis; microfilariae; patients; India
Abstract:
... India has still not eradicated filariasis, which is still endemic throughout the country. This study focuses on the breast as a non-typical but not uncommon site of breast disease characterized by nematodes causing obstruction of lymphatic vessels caused by filariasis. The case described herein involves a 26-year-old married female who presented with a subcutaneous nodule in her left breast. Clini ...
DOI:
10.1007/s12639-022-01470-w

28. Calumenin knockdown, by intronic artificial microRNA, to improve expression efficiency of the recombinant human coagulation factor IX

Author:
Javad Parnian; Maryam Hoseindokht; Zahra Khademi; Maedeh Moosavi; Zahra Soheila Soheili; Shahram Samie; Alireza Zomorodipour
Source:
Biotechnology letters 2022 v.44 no.5-6 pp. 713-728
ISSN:
0141-5492
Subject:
barium; biotechnology; citrates; coagulation; enzyme-linked immunosorbent assay; genetic vectors; human cell lines; humans; introns; microRNA
Abstract:
... OBJECTIVES: To improve the expression efficiency of recombinant hFIX, by enhancing its γ-carboxylation, which is inhibited by Calumenin (CALU), we used intronic artificial microRNAs (amiRNAs) for the CALU downregulation. METHODS: Two human CALU (hCALU)-specific amiRNAs were designed, validated and inserted within a truncated form of the hFIX intron 1, in either 3′- or 5′-untranslated regions of th ...
DOI:
10.1007/s10529-022-03249-8

29. Centrifugal therapeutic plasma exchange in dogs with immune‐mediated hemolytic anemia (2016–2018): 7 cases

Author:
Christine A. Culler; Alessio Vigani; Alexandra Z. Ripoll; Alexandra Gareau; Steven E. Suter
Source:
Journal of veterinary emergency and critical care 2022 v.32 no.5 pp. 645-652
ISSN:
1479-3261
Subject:
bilirubin; blood serum; citrates; databases; dogs; hemolytic anemia; hospitals; immunosuppression; therapeutics; North Carolina
Abstract:
... OBJECTIVE: To describe the technique of centrifugal therapeutic plasma exchange (cTPE) in dogs diagnosed with immune‐mediated hemolytic anemia (IMHA) and summarize the outcome of the procedure. DESIGN: Retrospective review of cTPE performed at North Carolina State University from 2016 to 2018, through a search of the institutional database for cTPE and IMHA. SETTING: University teaching hospital. ...
DOI:
10.1111/vec.13196

30. Characteristics and properties of the quaternary ammonium-functionalized micron chitosan modified by zinc citrate chelates for encapsulation of betanin

Author:
Jiahao Xu; Jiayi Lian; Lijun You; Zhengang Zhao
Source:
Colloids and surfaces 2022 v.218 pp. 112752
ISSN:
0927-7765
Subject:
antibacterial properties; antioxidant activity; breads; cell viability; chitosan; citrates; dietary supplements; encapsulation; food industry; food safety; hydrophilicity; ingredients; intestines; quaternary ammonium compounds; spoilage; synthesis; zinc
Abstract:
... Betanin is a high-value bioactive ingredient, but its application in the food industry is limited by its easy degradability. Encapsulation can improve the stability of various ingredients and is widely used in the food industry. In this work, chitosan modified by zinc citrate chelates (CS@Zn-CQDs) were synthesized by one-pot synthesis and the surface of them was quaternary ammonium modified to get ...
DOI:
10.1016/j.colsurfb.2022.112752

31. Characterization and functional significance of bacteria associated with rugose spiralling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) reared on guava plants

Author:
M. Saranya; J. S. Kennedy; R. Anandham; A. Manikandan
Source:
Applied entomology and zoology 2022 v.57 no.4 pp. 323-331
ISSN:
0003-6862
Subject:
Aleurodicus; Bacillus altitudinis; agar; chitinase; citrates; digestive system; entomology; fermentation; genes; guavas; hydrolysis; indoles; insects; lactose; phloem; proteinases; siderophores; skim milk; starch
Abstract:
... Rugose spiralling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) is an introduced phloem feeder of guava. Gut symbiotic bacteria alter the physiology of the host insect. The present study characterized the nine bacterial isolates from the second nymphal stage of rugose spiralling whitefly using seven different bacteria growth media viz., Nutrient Agar (NA), Luria Bertani aga ...
DOI:
10.1007/s13355-022-00791-9

32. Characterization of Alcaligenes aquatilis as a novel member of heterotrophic nitrifier-aerobic denitrifier and its performance in treating piggery wastewater

Author:
Xianhe Cao; Binhan Zhao; Yongming Wu; Jun Huang; Hongzhi Wang; Xianyun Sun; Shaojie Li
Source:
Bioresource technology 2022 v.354 pp. 127176
ISSN:
0960-8524
Subject:
Alcaligenes; acetates; activated sludge; ammonia; carbon; chemical oxygen demand; citrates; denitrification; denitrifying microorganisms; hydroxylamine; nitrites; oxidation; pH; succinic acid; technology; total nitrogen; wastewater
Abstract:
... A novel strain AS1 with heterotrophic nitrifying-aerobic denitrifying capacity in the species of Alcaligenes aquatilis was isolated from the aerobic activated sludge. It showed a great capability of ammonia removal, and the aerobic metabolic pathways to yield gaseous-nitrogen by hydroxylamine oxidation and nitrite denitrification were proposed. AS1 could efficiently remove ammonia under a wide ran ...
DOI:
10.1016/j.biortech.2022.127176

33. Characterization of a new heterotrophic nitrification bacterium Pseudomonas sp. strain JQ170 and functional identification of nap gene in nitrite production

Author:
Siqiong Xu; Fuyin Zhang; Yinhu Jiang; Kaiyun Zhang; Qing Hong; Jiguo Qiu; Jian He
Source:
Science of the total environment 2022 v.806 pp. 150556
ISSN:
0048-9697
Subject:
Pseudomonas; ammonia; bacteria; carbon; citrates; cytoplasm; environment; gene targeting; genes; hydroxylamine; insertional mutagenesis; nitrates; nitrification; nitrites; nitrogen; oxidoreductases; pH; pollution control; temperature; transposons
Abstract:
... Heterotrophic nitrification bacteria play a critical role in nitrogen cycling and pollution removal. However, the underlying nitrification mechanisms are diverse and have rarely been investigated at the genetic level. In this study, the new heterotrophic nitrifier Pseudomonas sp. strain JQ170 was isolated. Strain JQ170 can utilize ammonia (NH₄⁺-N), nitrite (NO₂⁻-N), or nitrate (NO₃⁻-N) as sole nit ...
DOI:
10.1016/j.scitotenv.2021.150556

34. Characterization of autochthonal yeasts isolated from Spanish soft raw ewe milk protected designation of origin cheeses for technological application

Author:
Almudena V. Merchán; Santiago Ruiz-Moyano; María Vázquez Hernández; María José Benito; Emilio Aranda; Alicia Rodríguez; Alberto Martín
Source:
Journal of dairy science 2022 v.105 no.4 pp. 2931-2947
ISSN:
0022-0302
Subject:
Debaryomyces hansenii; Kluyveromyces marxianus var. lactis; Pichia jadinii; Pichia kudriavzevii; Yarrowia lipolytica; agar; aminopeptidases; cheeses; citrates; enzyme activity; ewe milk; galactose; genotyping; glucose; internal transcribed spacers; lactic acid; lactose; lipolysis; principal component analysis; proteolysis; skim milk; starter cultures; traditional technology; tyrosine; yeasts; Spain
Abstract:
... The yeasts involved in the ripening process of artisanal soft raw ewe milk Protected Designation of Origin (PDO) Torta del Casar and Queso de la Serena cheeses produced in Extremadura, Spain, were isolated throughout their ripening process, strain typed, and characterized for some important technological properties. A total of 508 yeast isolates were obtained and identified by inter-single sequenc ...
DOI:
10.3168/jds.2021-21368

35. Characterization of copper-induced-release of exudates by Citrus sinensis roots and their possible roles in copper-tolerance

Author:
Huan-Huan Chen; Xu-Feng Chen; Zhi-Chao Zheng; Wei-Lin Huang; Jiuxin Guo; Lin-Tong Yang; Li-Song Chen
Source:
Chemosphere 2022 v.308 pp. 136348
ISSN:
0045-6535
Subject:
Citrus sinensis; alkalinization; citrates; electrolyte leakage; hydroponics; malates; malondialdehyde; oranges; pH; phenolic compounds; phytotoxicity; protein synthesis; temperature
Abstract:
... Copper (Cu) excess is often observed in old Citrus orchards. Little information is available on the characterization of Cu-induced-release of root exudates and their possible roles in plant Cu-tolerance. Using sweet orange [Citrus sinensis (L.) Osbeck cv. Xuegan] seedlings as materials, we investigated the impacts of 0, 0.5, 25, 150, 350, 550, 1000, 2000 or 5000 μM CuCl₂ (pH 4.8) on Cu uptake, roo ...
DOI:
10.1016/j.chemosphere.2022.136348

36. Characterization of kaolinite aqueous suspensions by acoustophoresis: Influence of crystallinity and of the ratio between basal and lateral surface of grains

Author:
Rana Al-Tahan; Richard Mayet; Patrice Duport; Nicolas Tessier-Doyen; Anne Aimable; Gilles Gasgnier; Cécile Pagnoux
Source:
Colloids and surfaces 2022 v.649 pp. 129473
ISSN:
0927-7757
Subject:
citrates; crystal structure; kaolin; kaolinite; raw materials; sodium; temperature; ultrasonics; zeta potential
Abstract:
... The electrokinetic properties of aqueous suspensions of two kaolins were studied using the acoustophoretic method by measuring the ultrasonic attenuation spectrum in a wide frequency range (1–20 MHz). Then, the zeta potential is calculated according to the size distribution of grains. A high state of crystallinity of the kaolinite platelets strongly influences the amplitude and the variation of ze ...
DOI:
10.1016/j.colsurfa.2022.129473

37. Characterization of nettle leaves (Urtica dioica) as a novel source of protease for clotting dromedary milk by non-destructive methods

Author:
Alia Bouazizi; Imène Felfoul; Hamadi Attia; Romdhane Karoui
Source:
Colloids and surfaces 2022 v.211 pp. 112312
ISSN:
0927-7765
Subject:
Urtica dioica; camel milk; chlorophyll; citrates; enzyme activity; fluorescence; fluorescence emission spectroscopy; milk clotting; pH; phosphates; proteinases; proteolysis
Abstract:
... This study investigates the valorization of the nettle leaves (Urtica dioica) as a novel source of a protease for clotting dromedary milk. The aim of this work is to study the effect of extracting pH on the enzymatic activity of nettle leaves extracts. The extraction was achieved in phosphate citrate buffer at different pH values (from 3 to 6.5) and the obtained extracts were used to coagulate dro ...
DOI:
10.1016/j.colsurfb.2021.112312

38. Characterization of tars from recycling of PHA bioplastic and synthetic plastics using fast pyrolysis

Author:
Alican Akgül; Tania Palmeiro-Sanchez; Heiko Lange; Duarte Magalhaes; Sean Moore; Alexandre Paiva; Feyza Kazanç; Anna Trubetskaya
Source:
Journal of hazardous materials 2022 v.439 pp. 129696
ISSN:
0304-3894
Subject:
bioplastics; carbon fibers; carcinogenicity; citrates; composite polymers; gas chromatography-mass spectrometry; gel chromatography; liquids; molecular weight; nuclear magnetic resonance spectroscopy; polyethylene terephthalates; polyhydroxyalkanoates; pyrolysis; temperature; thermal stability; thermogravimetry
Abstract:
... The aim of this study was to investigate the pyrolysis products of polyhydroxyalkanoates (PHAs), polyethylene terephthalate (PET), carbon fiber reinforced composite (CFRC), and block co-polymers (PS-b-P2VP and PS-b-P4VP). The studied PHA samples were produced at temperatures of 15 and 50 ᵒC (PHA15 and PHA50), and commercially obtained from GlasPort Bio (PHAc). Initially, PHA samples were analyzed ...
DOI:
10.1016/j.jhazmat.2022.129696

39. Characterizing the effect of S-nitrosoglutathione on Saccharomyces cerevisiae: Upregulation of alcohol dehydrogenase and inactivation of aconitase

Author:
Swarnab Sengupta; Rohan Nath; Arindam Bhattacharjee
Source:
Process biochemistry 2022 v.113 pp. 62-70
ISSN:
1359-5113
Subject:
Saccharomyces cerevisiae; aconitate hydratase; alcohol dehydrogenase; citrates; ethanol production; gene expression; genes
Abstract:
... When exposed to nitrosative stress, the redox status of Saccharomyces cerevisiae changes significantly in vivo. Under nitrosative stress, aconitase, which catalyzes the conversion of citrate to isocitrate in the tricarboxylic acid (TCA) cycle, is known to be vulnerable. In this study, aconitase was completely repressed in the presence of 0.25 mM S-nitrosoglutathione (GSNO) as the nitrosative stres ...
DOI:
10.1016/j.procbio.2021.12.011

40. Chemical and sensory discrimination of coffee: impacts of the planting altitude and fermentation

Author:
Emanuele Catarina da Silva Oliveira; José Maria Rodrigues da Luz; Marina Gomes de Castro; Paulo Roberto Filgueiras; Rogério Carvalho Guarçoni; Eustáquio Vinicius Ribeiro de Castro; Marliane de Cássia Soares da Silva; Lucas Louzada Pereira
Source:
European food research & technology 2022 v.248 no.3 pp. 659-669
ISSN:
1438-2377
Subject:
altitude; caffeine; citrates; discriminant analysis; fermentation; food research; formic acid; malates; microbiome; nuclear magnetic resonance spectroscopy; quinic acid; sensory properties; soil; trigonelline
Abstract:
... Edaphoclimatic conditions, planting altitudes, soil, the microbiome of plants and fruits, genotypes, and postharvest processing are variables that contribute to the chemical and sensory quality of the coffee. Thus, the objective of this study was to evaluate the impacts of planting altitude and fermentation of fruits on the chemical and sensory quality of the coffee using Nuclear Magnetic Resonanc ...
DOI:
10.1007/s00217-021-03912-w

41. Chemometric approaches to evaluate the substitution of synthetic food dyes by natural compounds: The case of nanoencapsulated curcumin, spirulina, and hibiscus extracts

Author:
Valéria Maria Costa Teixeira; Roberta França Gomes da Silva; Odinei Hess Gonçalves; Carla Pereira; Lillian Barros; Isabel C.F.R. Ferreira; Evandro Bona; Fernanda Vitória Leimann
Source:
Lebensmittel-Wissenschaft + Technologie 2022 v.154 pp. 112786
ISSN:
0023-6438
Subject:
Arthrospira platensis; Hibiscus sabdariffa; Spirulina; antioxidant activity; apricots; chemometrics; citrates; color; curcumin; nanocapsules; pH; phosphates; principal component analysis; strawberries; tartrazine; water solubility; yogurt
Abstract:
... Finding natural food coloring options from plant-based sources to substitute artificial dyes is a challenging task because natural dyes often present low water-solubility, not very vibrant hues, and instability due to interactions with food ingredients. Chemometric approaches can be used to evaluate color differences and patterns resulting from natural and synthetic dyes when applied to food syste ...
DOI:
10.1016/j.lwt.2021.112786

42. Chitosan-based Dy2O3/CuFe3O4 bio-nanocomposite development, characterization, and drug release kinetics

Author:
Ayesha Anwar; Muhammad Imran; Muhammad Ramzan; Farhan A. Khan; Nimra Ismail; Abdullah Ijaz Hussain; Syed Makhdoom Hussain; Walaa F. Alsanie; Hafiz M.N. Iqbal
Source:
International journal of biological macromolecules 2022 v.220 pp. 788-801
ISSN:
0141-8130
Subject:
absorbance; chemical structure; chitosan; ciprofloxacin; citrates; crystallites; dysprosium; nanocarriers; nanocomposites; pH; pharmacokinetics; ultraviolet-visible spectroscopy; zeta potential
Abstract:
... Chitosan (CS)/metal oxide (MO) nano-carriers have recently attracted attention due to their great integration into several biomedical applications. Herein, CS and dysprosium oxide based bio-nanocomposites (Dy₂O₃/CuFe₃O₄/CS) were prepared using a citrate sol-gel route for biomedical settings at large and drug delivery, in particular. The chemical structure, average crystallite size, and surface mor ...
DOI:
10.1016/j.ijbiomac.2022.08.119

43. Cholinium-Based Ionic Liquids as Efficient Media for Improving the Structural and Thermal Stability of Immunoglobulin G Antibodies

Author:
Diksha Dhiman; Meena Bisht; Ana P. M. Tavares; Mara G. Freire; Pannuru Venkatesu
Source:
ACS sustainable chemistry & engineering 2022 v.10 no.17 pp. 5404-5420
ISSN:
2168-0485
Subject:
Gibbs free energy; acetates; ambient temperature; aqueous solutions; biopharmaceuticals; citrates; fluorescence; fluorescence emission spectroscopy; green chemistry; high performance liquid chromatography; hydrodynamics; immunoglobulin G; phosphates; polyacrylamide gel electrophoresis; quantitative analysis; sodium; therapeutics; thermal stability
Abstract:
... Among antibody-based biopharmaceuticals, immunoglobulin G (IgG) plays a central role. However, IgG is a protein and as such easily loses stability, thus compromising its use as an effective therapeutic. To overcome this drawback, in this work, we evaluated cholinium-based ionic liquids (ILs) as effective stabilizers of IgG. We have investigated four ILs in aqueous solution, namely, cholinium aceta ...
DOI:
10.1021/acssuschemeng.1c07979

44. Circulating metabolites in the early stage of breast cancer were not related to cancer stage or subtypes but associated with ki67 level. Promising statistical discrimination from controls

Author:
Eva Baranovicova; Peter Racay; Pavol Zubor; Marek Smolar; Eva Kudelova; Erika Halasova; Dana Dvorska; Zuzana Dankova
Source:
Molecular and cellular probes 2022 pp. 101862
ISSN:
0890-8508
Subject:
3-hydroxybutyric acid; alanine; biomarkers; blood plasma; breast neoplasms; citrates; diagnostic techniques; glutamine; histidine; histology; metabolites; metabolomics; proline; pyruvic acid; tyrosine
Abstract:
... It was documented that the presence of malignancy in an organism causes metabolomic alterations in blood plasma which applies also to breast cancer. Breast cancer is a heterogeneous disease and there are only limited known relations of plasma metabolomic signatures with the tumour characteristics in early BC and knowing them would be of great advantage in noninvasive diagnostics. In this study, we ...
DOI:
10.1016/j.mcp.2022.101862

45. Citrate stabilised silver nanoparticles as sensing probe for in-situ Sn²⁺ ion determination

Author:
Jamil K. Salem; Issa M. El Nahhal; Mohammed H. Shurrab
Source:
International journal of environmental analytical chemistry 2022 v.102 no.4 pp. 844-854
ISSN:
1029-0397
Subject:
absorbance; absorption; ambient temperature; analytical chemistry; citrates; detection limit; ionic strength; nanosilver; particle size
Abstract:
... We present a simple method for the determination of Sn²⁺ concentration using stannous-synthesised AgNPs as sensing probe at room temperature. The average particle size of stannous-synthesised AgNPs obtained from TEM analysis was about 14 nm with a symmetrical absorption band at 405 nm. The detection method was based on the formation of stable AgNPs using Sn²⁺ ion as reducing agent and trisodium ci ...
DOI:
10.1080/03067319.2020.1726901

46. Citrate-modified biochar for simultaneous and efficient plant-available silicon release and copper adsorption: Performance and mechanisms

Author:
Yang Liu; Lixun Zhang; Zhengfang Zhang; Ying Zhang; Yuntao Guan
Source:
Journal of environmental management 2022 v.301 pp. 113819
ISSN:
0301-4797
Subject:
Lewis bases; adsorption; agricultural soils; agroecosystems; biochar; biomass; citrates; electrostatic interactions; environmental management; hydrogen; hydrolysis; pollution; pyrolysis; silicates; silicon; soil conditioners
Abstract:
... Silicon (Si) deficiency and heavy metals (HMs) pollution are common for farmland soil because of long-term intensive farming. In this study, a novel citrate-modified biochar (C-BC) was introduced as a soil conditioner to simultaneously increase the amount of plant-available Si (PASi) and immobilize HMs. The maximum amount of PASi released was 33.00 mg⋅g⁻¹ from C-BC pre-treated with 0.1 mol⋅L⁻¹ cit ...
DOI:
10.1016/j.jenvman.2021.113819

47. Citrus heat shock transcription factor CitHsfA7‐mediated citric acid degradation in response to heat stress

Author:
Shao‐jia Li; Sheng‐chao Liu; Xia‐hui Lin; Donald Grierson; Xue‐ren Yin; Kun‐song Chen
Source:
Plant, cell and environment 2022 v.45 no.1 pp. 95-104
ISSN:
0140-7791
Subject:
Citrus; air; citrates; citric acid; citrus fruits; environment; fruits; genes; heat stress; metabolism; transcription (genetics); transcription factors; transcriptional activation
Abstract:
... Heat stress is a major abiotic stress for plants, which can generate a range of biochemical and genetic responses. In ‘Ponkan’ mandarin fruit, hot air treatment (HAT) accelerates the degradation of citric acid. However, the transcriptional regulatory mechanisms of citrate degradation in response to HAT remain to be elucidated. Here, 17 heat shock transcription factor sequences were isolated, and d ...
DOI:
10.1111/pce.14207

48. Co-existence of type I fatty acid synthase and polyketide synthase metabolons in Aurantiochytrium SW1 and their implications for lipid biosynthesis

Author:
Shuwahida Shuib; Mohamed Yusuf Mohamed Nazir; Izyanti Ibrahim; Yuanda Song; Colin Ratledge; Aidil Abdul Hamid
Source:
Biochimica et biophysica acta 2022 v.1867 no.12 pp. 159224
ISSN:
1388-1981
Subject:
NADP-glucose-6-phosphate dehydrogenase; acetyl-CoA carboxylase; biosynthesis; citrates; dissociation; docosahexaenoic acid; docosapentaenoic acid; fatty-acid synthase; malate dehydrogenase; malic enzyme; palmitic acid; phosphogluconate dehydrogenase; polyketide synthases; polyketides; pyruvate carboxylase
Abstract:
... The key enzymes of lipid biosynthesis in oleaginous filamentous fungi exist as metabolons. However, the existence of a similar organization in other groups of oleaginous microorganisms is still unknown. In this study, we confirmed the occurrence of two separate and distinct lipogenic metabolons in a thraustochytrid, Aurantiochytrium SW1. These involve the Type I Fatty Acid Synthase (FAS) pathway, ...
DOI:
10.1016/j.bbalip.2022.159224

49. Colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables

Author:
Kuo-Hui Wu; Wen-Chien Huang; Shu-Chen Chang; Rong-Hwa Shyu
Source:
RSC advances 2022 v.12 no.21 pp. 13035-13044
ISSN:
2046-2069
Subject:
citrates; cross reaction; detection limit; immunoassays; liquids; methamidophos; nanosilver; omethoate; pH; pesticide residues; polyclonal antibodies; profenofos; rapid methods
Abstract:
... A colloidal silver nanoparticle (AgNP)-based lateral flow immunoassay (LFIA) was evaluated in terms of the rapid detection of profenofos (PEO) pesticide residue in vegetables. Colloidal AgNPs, of a diameter of approximately 20 nm, were surface-modified with trisodium citrate dehydrate (TSC) in order to improve their stability and dispersion. An anti-profenofos polyclonal antibody (pAb) was success ...
DOI:
10.1039/d2ra01654k

50. Colorimetric indicators of açaí anthocyanin extract in the biodegradable polymer matrix to indicate fresh shrimp

Author:
Samiris Côcco Teixeira; Taíla Veloso de Oliveira; Rafael Resende Assis Silva; Alane Rafaela Costa Ribeiro; Paulo César Stringheta; Thaís Caroline Buttow Rigolon; Marcos Roberto Moacir Ribeiro Pinto; Nilda de Fátima Ferreira Soares
Source:
Food bioscience 2022 v.48 pp. 101808
ISSN:
2212-4292
Subject:
ammonia; anthocyanins; biodegradability; citrates; color; colorimetry; electron microscopy; food spoilage; food waste; freshness; glycerol; pH; polymers; shrimp; spoilage; ultraviolet-visible spectroscopy
Abstract:
... Colorimetric indicator or freshness indicators hold great potential to reduce food waste. Films off açaí anthocyanins extract (AE) with different concentrations molars of two plasticizers glycerol (GLY) or triethyl citrate (TEC), for application fresh shrimp, real time were evaluated. The AE undergoes a visible pink/violet to green/yellowish color change in response to different pH's, which is an ...
DOI:
10.1016/j.fbio.2022.101808