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You searched for: Subject coculture Remove constraint Subject: coculture Subject exosomes Remove constraint Subject: exosomes
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1. Acute lymphoblastic leukemia-derived exosome inhibits cytotoxicity of natural killer cells by TGF-β signaling pathway

Author:
Huijun Yu; Tingting Huang; Daming Wang; Lei Chen; Xi Lan; Xintong Liu; Keyan Chen; Haihong He; Shaobo Li; Yiwen Zhou; Jiansheng Xie
Source:
3 Biotech 2021 v.11 no.7 pp. 313
ISSN:
2190-572X
Subject:
Western blotting; coculture; cytotoxicity; enzyme-linked immunosorbent assay; exosomes; fluorescent antibody technique; gene expression; immunotherapy; lymphocytic leukemia; protein kinases; viability
Abstract:
... This study was conducted to explore whether acute lymphoblastic leukemia (ALL)-derived exosomes affect natural killer (NK) cells. Exosomes were isolated and identified from Jurkat cells and co-cultured with NK cells. Then, the cytotoxicity, viability, and release of perforin and granzyme B in NK92-MI cells were measured. PCR arrays were used to detect gene expression alterations in the transformin ...
DOI:
10.1007/s13205-021-02817-5

2. Adipocytes‐Derived Exosomal microRNA‐1224 Inhibits M2 Macrophage Polarization in Obesity‐Induced Adipose Tissue Inflammation via MSI2‐Mediated Wnt/β‐Catenin Axis

Author:
Dongdong Zhang; Xiaoyan Yao; Yaqin Teng; Tiantian Zhao; Liangyan Lin; Yuanyuan Li; Hongxia Shang; Yongjun Jin; Qingsong Jin
Source:
Molecular nutrition & food research 2022 v.66 no.18 pp. e2100889
ISSN:
1613-4125
Subject:
RNA; adipocytes; coculture; epididymis; exosomes; flow cytometry; food research; high fat diet; immunohistochemistry; inflammation; macrophages; phenotype; white adipose tissue
Abstract:
... SCOPE: Phenotypic switch of macrophage polarization in adipose tissue has been associated with obesity‐induced adipose tissue inflammation (OATI). Therefore, this study aims to explore the possible mechanism of adipocytes‐derived exosomes (ADEs) carrying microRNA‐1224 (miR‐1224) in M2 macrophage polarization of OATI. METHODS AND RESULTS: miR‐1224‐knockout (miR‐1224‐KO) mice for this study, and iso ...
DOI:
10.1002/mnfr.202100889

3. Adipose mesenchymal stem cell-derived exosomes stimulated by hydrogen peroxide enhanced skin flap recovery in ischemia-reperfusion injury

Author:
Yun Bai; Yu-di Han; Xin-long Yan; Jing Ren; Quan Zeng; Xiao-dong Li; Xue-tao Pei; Yan Han
Source:
Biochemical and biophysical research communications 2018 v.500 no.2 pp. 310-317
ISSN:
0006-291X
Subject:
angiogenesis; apoptosis; blood; coculture; eosin; exosomes; human umbilical vein endothelial cells; hydrogen peroxide; image analysis; immunohistochemistry; inflammation; ischemia; mesenchymal stromal cells; staining; stem cells; therapeutics
Abstract:
... Mesenchymal stem cell (MSC)-derived exosomes have been recognized as new candidates for the treatment of ischemic disease or injury and may be an alternative treatment for cell therapy. This aim of the study was to evaluate whether exosomes derived from adipose mesenchymal stem cell (ADSC) can protect the skin flap during ischemia-reperfusion (I/R) injury and induce neovascularization.To investiga ...
DOI:
10.1016/j.bbrc.2018.04.065

4. Astrocytes promote the proliferation of oligodendrocyte precursor cells through connexin 47-mediated LAMB2 secretion in exosomes

Author:
Nannan Cheng; Yuanfeng Xiong; Wenjin Zhang; Xiaohong Wu; Zhongxiang Sun; Lei Zhang; Hong Wu; Yong Tang; Yan Peng
Source:
Molecular biology reports 2022 v.49 no.8 pp. 7263-7273
ISSN:
0301-4851
Subject:
DNA replication; Western blotting; astrocytes; brain; coculture; connexins; cyclins; exosomes; gene ontology; interphase; mass spectrometry; molecular biology; oligodendroglia; secretion; transcriptome; transmission electron microscopy
Abstract:
... BACKGROUND: Oligodendrocyte precursor cells (OPCs) can proliferate and differentiate into oligodendrocytes, the only myelin-forming cells in the central nervous system. Proliferating OPCs promotes remyelination in neurodegenerative diseases. Astrocytes (ASTs) are the most widespread cells in the brain and play a beneficial role in the proliferation of OPCs. Connexin 47 (Cx47) is the main component ...
DOI:
10.1007/s11033-022-07508-9

5. Bone marrow mesenchymal stem cell-derived exosomes enhance osteoclastogenesis during alveolar bone deterioration in rats

Author:
Shuyu Xu; Zuolin Wang
Source:
RSC advances 2017 v.7 no.34 pp. 21153-21163
ISSN:
2046-2069
Subject:
animal models; bone marrow; bone resorption; cell lines; coculture; dentures; elderly; exosomes; females; mesenchymal stromal cells; osteoclasts; osteoporosis; ovariectomy; pathogenesis; patients; rats; synergism
Abstract:
... Healthy and functional alveolar bones to support implants or dentures are fundamental for dental restoration. Osteoporosis, which leads to alveolar bone resorption, is a common disease, especially in elderly patients with tooth loss. Enhanced osteoclastogenesis is the main pathogenesis of bone resorption, which is related to the paracrine regulation of BMMSCs (bone marrow mesenchymal stem cells). ...
DOI:
10.1039/c6ra27931g

6. Bone marrow mesenchymal stem cells-derived exosomal microRNA-19b-1-5p reduces proliferation and raises apoptosis of bladder cancer cells via targeting ABL2

Author:
Dewang Fu; Ben Liu; Huamao Jiang; Zhaowei Li; Chenghui Fan; Li'e Zang
Source:
Genomics 2021 v.113 no.3 pp. 1338-1348
ISSN:
0888-7543
Subject:
apoptosis; bone marrow; cell communication; coculture; exosomes; genomics; microRNA; tyrosine; urinary bladder neoplasms
Abstract:
... Exosomes are involved in intercellular communication via specialized molecular cargo, such as microRNAs (miRNAs). However, the mechanisms underlying exosomal miR-19b-1-5p in bladder cancer remain largely unknown, thus, we aim to investigate the effect of exosomal miR-19b-1-5p on bladder cancer with the involvement of non-receptor protein tyrosine kinase Arg (ABL2). miR-19b-1-5p and ABL2 expression ...
DOI:
10.1016/j.ygeno.2021.03.011

7. Brown adipocyte-derived exosomal miR-132-3p suppress hepatic Srebf1 expression and thereby attenuate expression of lipogenic genes

Author:
Yuichi Kariba; Tatsuya Yoshizawa; Yoshifumi Sato; Tomonori Tsuyama; Eiichi Araki; Kazuya Yamagata
Source:
Biochemical and biophysical research communications 2020 v.530 no.3 pp. 500-507
ISSN:
0006-291X
Subject:
brown adipocytes; brown adipose tissue; coculture; cold; cold stress; exosomes; gene expression; lipogenesis; liver; microRNA; neurons; neurotransmitters; norepinephrine; research; sympathetic nervous system
Abstract:
... Recent evidence has revealed a novel signaling mechanism through which brown adipose tissue (BAT)-derived exosomal microRNAs (miRNAs) influence hepatic gene expression. Here, we uncover neuronal control of these miRNAs and identify exosomal miR-132-3p as a regulator of hepatic lipogenesis under cold stress conditions. Norepinephrine, a sympathetic nervous system neurotransmitter mediating cold-ind ...
DOI:
10.1016/j.bbrc.2020.05.090

8. Cancer-associated fibroblasts-derived exosomes promote lung cancer progression by OIP5-AS1/ miR-142-5p/ PD-L1 axis

Author:
Yun Jiang; Kun Wang; Xiaoning Lu; Yongliang Wang; Jianle Chen
Source:
Molecular immunology 2021 v.140 pp. 47-58
ISSN:
0161-5890
Subject:
Western blotting; apoptosis; bioinformatics; carcinogenesis; cell communication; coculture; exosomes; fibroblasts; flow cytometry; genes; immunosuppression; ligands; luciferase; lung neoplasms; microRNA; microarray technology; models; neoplasm progression; non-coding RNA; subcutaneous injection; transmission electron microscopy
Abstract:
... Cancer-associated fibroblasts (CAFs) are the most important stromal cells in the tumor microenvironment (TEM) and have been reported to regulate various cancer development. Exosomes are considered important elements involved in intercellular communication and TME regulation, while the potential function of CAFs in lung cancer immunosuppressive microenvironments remains unknown. CAFs-derived exosom ...
DOI:
10.1016/j.molimm.2021.10.002

9. Cryopreservation of mesenchymal stromal cell-derived extracellular vesicles using trehalose maintains their ability to expand hematopoietic stem cells in vitro

Author:
Pallavi Budgude; Vaijayanti Kale; Anuradha Vaidya
Source:
Cryobiology 2021 v.98 pp. 152-163
ISSN:
0011-2240
Subject:
cell communication; chemotaxis; coculture; cryobiology; cryopreservation; exosomes; hematopoiesis; liver; liver failure; medicine; secretion; spleen; storage time; therapeutics; trehalose
Abstract:
... The multitude of clinical trials using mesenchymal stromal cells (MSCs) has underscored their significance as a promising cell source for regenerative therapies. Most studies have however shown that MSCs get entrapped into the microvasculature of lungs, liver and spleen. In addition to intercellular communication, MSCs exert their effects in a paracrine manner by secretion of extracellular vesicle ...
DOI:
10.1016/j.cryobiol.2020.11.009

10. DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1

Author:
Shuang Li; Anjing Zhu; Kai Ren; Shilin Li; Limin Chen
Source:
Life sciences 2020 v.263 pp. 118564
ISSN:
0024-3205
Subject:
DNA replication; Zika virus; cell communication; cell cycle; cell proliferation; coculture; exosomes; flow cytometry; humans; innate immunity; pathogenicity; public health; transcriptome; virus replication; viruses
Abstract:
... Zika virus (ZIKV) infection causes a public health concern because of its potential association with the development of microcephaly. During viral infections, the host innate immune response is mounted quickly to produce some endogenous functional molecules to limit virus replication and spread. Exosomes contain molecules from their cell of origin following virus infection and can enter recipient ...
DOI:
10.1016/j.lfs.2020.118564

11. Deoxycholic acid-stimulated macrophage-derived exosomes promote spasmolytic polypeptide-expressing metaplasia in the stomach

Author:
Xianjun Xu; Jinnian Cheng; Shengzheng Luo; Xiaoyuan Gong; Dan Huang; Jingxian Xu; Yueqin Qian; Xinjian Wan; Hui Zhou
Source:
Biochemical and biophysical research communications 2020 v.524 no.3 pp. 649-655
ISSN:
0006-291X
Subject:
bile; coculture; deoxycholic acid; exosomes; fluorescent antibody technique; inflammation; intestines; intragastric administration; lectins; macrophages; metaplasia; organoids; parasympatholytics; quantitative polymerase chain reaction; research; risk factors; stomach; stomach neoplasms; ultracentrifugation
Abstract:
... Spasmolytic polypeptide-expressing metaplasia (SPEM) is an important risk factor for the occurrence of gastric cancer. It may be driven by a chronic inflammatory environment in which macrophage is involved. Studies have shown that intestinal metaplasia may originate from SPEM, and bile acid-induced chronic inflammation plays an important role in the process of intestinal metaplasia. However, wheth ...
DOI:
10.1016/j.bbrc.2020.01.159

12. Deregulation of miR‐27a may contribute to canine fibroblast activation after coculture with a mast cell tumour cell line

Author:
Matias Aguilera‐Rojas; Soroush Sharbati; Torsten Stein; Ralf Einspanier
Source:
FEBS Open Bio 2020 v.10 no.5 pp. 802-816
ISSN:
2211-5463
Subject:
RNA interference; actin; carcinogenesis; cell growth; cell lines; coculture; cyclins; dogs; exosomes; extracellular matrix; fibroblasts; interleukin-6; mast cells; microRNA; muscles; neoplasm cells; neoplasms; protein content
Abstract:
... The tumour microenvironment comprises a diverse range of cells, including fibroblasts, immune cells and endothelial cells, along with extracellular matrix. In particular, fibroblasts are of significant interest as these cells are reprogrammed during tumorigenesis to become cancer‐associated fibroblasts (CAFs), which in turn support cancer cell growth. MicroRNAs (miRNAs) have been shown to be invol ...
DOI:
10.1002/2211-5463.12831

13. Effect of modified Jianpi Yangzheng on regulating content of PKM2 in gastric cancer cells-derived exosomes

Author:
Jian Wu; Mengyun Yuan; Junyu Shen; Yuxuan Chen; Ruijuan Zhang; Xu Chen; Haidan Wang; Zhonghua Yin; Xingxing Zhang; Shenlin Liu; Qingmin Sun
Source:
Phytomedicine 2022 v.103 pp. 154229
ISSN:
0944-7113
Subject:
Oriental traditional medicine; Western blotting; apoptosis; bioinformatics; blood serum; coculture; exosomes; flow cytometry; fluorescent antibody technique; glycolysis; macrophages; models; neoplasm progression; pharmacology; protein synthesis; pyruvate kinase; stomach neoplasms; xenotransplantation
Abstract:
... Modified Jianpi Yangzheng decoction (mJPYZ), as an empirical decoction of Traditional Chinese medicine has been shown significantly to prolong the survival of patients with advanced stage gastric cancer. Pyruvate kinase M2 (PKM2), has attracted attention for its important role on cellular aerobic glycolysis, however, few studies focus on PKM2 non-metabolic roles in tumor progression. Our study aim ...
DOI:
10.1016/j.phymed.2022.154229

14. Effects of purified exosome product on rotator cuff tendon-bone healing in vitro and in vivo

Author:
Ye Ren; Shuwei Zhang; Yicun Wang; Daniel S. Jacobson; Ramona L. Reisdorf; Tomoyuki Kuroiwa; Atta Behfar; Steven L. Moran; Scott P. Steinmann; Chunfeng Zhao
Source:
Biomaterials 2021 v.276 pp. 121019
ISSN:
0142-9612
Subject:
adults; biocompatible materials; biomechanics; coculture; exosomes; females; histology; models; osteoblasts; surgery; tenocytes
Abstract:
... Exosomes have multiple therapeutic targets, but the effects on healing rotator cuff tear (RCT) remain unclear. As a circulating exosome, purified exosome product (PEP) has the potential to lead to biomechanical improvement in RCT. Here, we have established a simple and efficient approach that identifies the function and underlying mechanisms of PEP on cell-cell interaction using a co-culture model ...
DOI:
10.1016/j.biomaterials.2021.121019

15. Exosomal miR-22-3p from human umbilical cord blood-derived mesenchymal stem cells protects against lipopolysaccharid-induced acute lung injury

Author:
Yuanfang Zheng; Jiyuan Liu; Ping Chen; Lu Lin; Yinzhu Luo; Xiaoying Ma; Jincai Lin; Ying Shen; Liyan Zhang
Source:
Life sciences 2021 v.269 pp. 119004
ISSN:
0024-3205
Subject:
animal models; apoptosis; breathing; cell proliferation; coculture; exosomes; humans; lungs; oxidative stress; rats; stress response; therapeutics; umbilical cord
Abstract:
... Mesenchymal stem cells (MSCs) are widely applied in various clinical disorders, including acute lung injury (ALI). We aimed to investigate the effects of human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs)-derived exosomal microRNA-22-3p (miR-22-3p) on lipopolysaccharid (LPS)-induced ALI via regulating frizzled class receptor 6 (FZD6). Rat lung cells were selected to construct the ...
DOI:
10.1016/j.lfs.2020.119004

16. Exosomal miRNA-486-5p derived from rheumatoid arthritis fibroblast-like synoviocytes induces osteoblast differentiation through the Tob1/BMP/Smad pathway

Author:
Jie Chen; Mao Liu; Xiao Luo; Lihui Peng; Zixia Zhao; Chengsong He; Yue He
Source:
Biomaterials science 2020 v.8 no.12 pp. 3430-3442
ISSN:
2047-4849
Subject:
alizarin; biocompatible materials; coculture; collagen; disease severity; exosomes; mice; microRNA; osteoblasts; pathogenesis; patients; rheumatoid arthritis
Abstract:
... The pathogenesis of rheumatoid arthritis (RA) is related to the inhibition of osteoblast differentiation. Exosomes secreted from RA fibroblast-like synoviocytes (RA-FLSs-exos) are associated with the pathogenesis of RA and microRNAs (miRNAs) being crucial for RA progression. Accordingly, the aim of the present study is to elucidate the effect of RA-FLS-derived exosomes on osteoblast differentiatio ...
DOI:
10.1039/c9bm01761e

17. Exosomal release of microRNA-454 by breast cancer cells sustains biological properties of cancer stem cells via the PRRT2/Wnt axis in ovarian cancer

Author:
Ling Wang; Miao He; Li Fu; Yuemei Jin
Source:
Life sciences 2020 v.257 pp. 118024
ISSN:
0024-3205
Subject:
blood flow; breast neoplasms; cell growth; cell viability; coculture; exosomes; genes; mice; microRNA; microarray technology; ovarian neoplasms; transmembrane proteins
Abstract:
... Cancer-derived exosomes carrying tumor-derived molecules such as miRNAs and proteins related to various phenotypes have been detected in both the bloodstream and other biofluids of patients with different cancers. Thus, our main purpose here was to determine the role of the exosomal microRNA-454 (miR-454) derived by MDA-MB-231 in self-renewal of cancer stem cells (CSCs) in ovarian cancer (OC). Ext ...
DOI:
10.1016/j.lfs.2020.118024

18. Exosome-shuttled miR-7162-3p from human umbilical cord derived mesenchymal stem cells repair endometrial stromal cell injury by restricting APOL6

Author:
Qin Shi; Di Wang; Xiaoying Ding; Xiaoqing Yang; Yuquan Zhang
Source:
Archives of biochemistry and biophysics 2021 v.707 pp. 108887
ISSN:
0003-9861
Subject:
Western blotting; apoptosis; biophysics; coculture; computer software; endometrium; exosomes; flow cytometry; humans; microRNA; microarray technology; models; therapeutics; transmission electron microscopy; umbilical cord
Abstract:
... Recent studies have shown that exosomes (Exos) derived from stem cells can be used as paracrine factors to regenerate cells and tissues via shuttling miRNAs. Exos derived from human umbilical cord derived mesenchymal stem cells (UCMSCs) have been found to alleviate mifepristone-induced endometrial stromal cell (ESC) injury in vitro. Information on the functions and mechanisms of Exos from UCMSC-in ...
DOI:
10.1016/j.abb.2021.108887

19. Exosomes derived from astrocytes after oxygen-glucose deprivation promote differentiation and migration of oligodendrocyte precursor cells in vitro

Author:
Yaping Xu; Yeye Tian; Yao Wang; Li Xu; Guini Song; Qiao Wu; Wei Wang; Minjie Xie
Source:
Molecular biology reports 2021 v.48 no.7 pp. 5473-5484
ISSN:
0301-4851
Subject:
astrocytes; brain damage; centrifugation; chemotaxis; coculture; exosomes; glutamic acid; hypoxia; inflammation; molecular biology; myelin sheath; oligodendroglia; oxidative stress; therapeutics
Abstract:
... BACKGROUND: Excessive release of glutamate, oxidative stress, inflammation after ischemic brain injury can lead to demyelination. Astrocytes participate in the maturation and differentiation of oligodendrocyte precursor cells (OPCs), and play multiple roles in the process of demyelination and remyelination. Here, we studied the role of Astrocyte-derived exosomes (AS-Exo) under ischemic conditions ...
DOI:
10.1007/s11033-021-06557-w

20. Exosomes derived from human umbilical vein endothelial cells promote neural stem cell expansion while maintain their stemness in culture

Author:
Yi-Zhe Zhang; Fan Liu; Chang-Geng Song; Xiu-Li Cao; Yu-Fei Zhang; Hai-Ning Wu; Chen-Jun Guo; Yong-Qiang Li; Qi-Jun Zheng; Min-Hua Zheng; Hua Han
Source:
Biochemical and biophysical research communications 2018 v.495 no.1 pp. 892-898
ISSN:
0006-291X
Subject:
adults; apoptosis; brain; cell communication; coculture; exosomes; gene expression regulation; genes; human umbilical vein endothelial cells; mice; stem cells
Abstract:
... The neural stem cell (NSC) niche in subventricular zone (SVZ) of adult mammalian brain contains dense vascular plexus, where endothelial cells (ECs) regulate NSCs by releasing plenty of angiocrine factors. However, the role of ECs-derived exosomes, a novel type of mediators of intercellular communications, in the regulation of NSCs remains unclear. In the current study, primary NSCs isolated from ...
DOI:
10.1016/j.bbrc.2017.11.092