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You searched for: Subject cryopreservation Remove constraint Subject: cryopreservation Subject term case studies Remove constraint Subject term: case studies Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Integration of somatic embryogenesis in a tree breeding programme: a case study with Picea abies

Author:
Hogberg, K.A.; Ekberg, I.; Norell, L.; Arnold, S. von.
Source:
Canadian journal of forest research = 1998 v.28 no.10 pp. 1536-1545
ISSN:
0045-5067
Subject:
tree breeding; cryopreservation; seedlings; embryo (plant); phenology; plant characteristics; height; Picea abies; somatic embryogenesis; agricultural programs and projects; genotype; micropropagation; yields; case studies; tissue culture; Sweden
Abstract:
... Potential for integration of somatic embryogenesis in a breeding programme of Norway spruce (Picea abies (L.) Karst.), and its limitations, were investigated in a case study with an ordinary breeding population. A standard protocol was used for initiation, proliferation, and cryopreservation of embryogenic cultures; maturation of somatic embryos; and plant regeneration. This is a practical necessi ...
DOI:
10.1139/x98-137

2. Case report: the effect of sperm number, concentration, and volume of insemination dose of chilled, stored, and transported semen on pregnancy rate in Standardbred mares

Author:
Newcombe, J.R.; Lichtwark, S.; Wilson, M.C.
Source:
Journal of equine veterinary science 2005 v.25 no.2 pp. 70-75
ISSN:
0737-0806
Subject:
mares; Standardbred; artificial insemination; spermatozoa; cold storage; cryopreservation; semen; pregnancy rate; male fertility; storage time; storage quality; case studies

3. Pulmonary cryptococcosis in slaughtered sheep: Anatomopathology and culture

Author:
Lemos, L.S.; Santos, A.S. de O. dos; Vieira-da-Motta, O.; Texeira, G.N.; Carvalho, E.C.Q. de
Source:
Veterinary microbiology 2007 v.125 no.3-4 pp. 350-354
ISSN:
0378-1135
Subject:
sheep; sheep diseases; respiratory tract diseases; mycoses; Cryptococcus neoformans; disease detection; case studies; epidemiological studies; pathogen identification; lungs; tissue analysis; cryopreservation; histopathology; yeasts; epithelium; viability; microscopy; differential staining; in vitro culture; risk assessment; disease transmission; zoonoses; Brazil
Abstract:
... Cryptococcosis is considered a zoonosis but is rare in stock animals. In humans it is imputed to be a mortality cause in immunocompromised patients. This case study reports the anatomopathology of respiratory cryptococcosis in ovine and the recuperation and identification of Cryptococcus neoformans var. neoformans from 1-year frozen lung tissue sample. The findings from haematoxylin/eosin staining ...
DOI:
10.1016/j.vetmic.2006.11.028

4. Plasma and Brain Cholinesterase in Methomyl-Intoxicated Free-Ranging Pigeons (Columba Livia f. Domestica)

Author:
David Villar; Dubel Balvin; Carlos Giraldo; Miguel Motas; Marta Olivera
Source:
Journal of veterinary diagnostic investigation v.22 no.2 pp. 313-315
ISSN:
1040-6387
Subject:
Columba livia; wild birds; poisoning; methomyl; mortality; signs and symptoms (animals and humans); cholinesterase; cholinesterase inhibitors; reference standards; enzyme activity; autolysis; brain; air temperature; cryopreservation; enzyme activation; quantitative analysis; case studies; temporal variation
Abstract:
... A mortality event caused by exposure to the carbamate insecticide methomyl was diagnosed in several hundred pigeons fed treated corn kernels in a city park. A cholinesterase inhibitor insecticide was initially suspected based on clinical signs and a significant inhibition (P < 0.05) of brain cholinesterase (ChE) activity compared with normal values for the species. However, brain ChE activity was ...
DOI:
10.1177/104063871002200229
PubMed:
20224101

5. Use of Direct Thaw Insemination to Establish Pregnancies with Frozen–Thawed Semen from a Standard Jack

Author:
Jepsen, Rebecca J.; Evans, Lawrence E.; Youngs, Curtis R.
Source:
Journal of equine veterinary science 2010 v.30 no.11 pp. 651-656
ISSN:
0737-0806
Subject:
asses; males; spermatozoa; artificial insemination; pregnancy; cryopreservation; thawing; fertilization (reproduction); cryoprotectants; cholesterol; semen extenders; male fertility; viability; case studies; semen; mares; ovarian follicles; human chorionic gonadotropin; ovulation; cyclodextrins
DOI:
10.1016/j.jevs.2010.10.001

6. Conservation in vitro of rare and threatened ferns--case studies of biodiversity hotspot and island species

Author:
Barnicoat, Helen; Cripps, Ryan; Kendon, Jonathan; Sarasan, Viswambharan
Source:
In vitro cellular & developmental biology 2011 v.47 no.1 pp. 37-45
ISSN:
1054-5476
Subject:
biodiversity; biotechnology; botanical gardens; case studies; conservation programs; cryopreservation; extinction; ferns and fern allies; flora; genetic variation; in vitro culture; indigenous species; islands; micropropagation; seeds; spores
Abstract:
... The importance of in vitro tools to complement other ex situ methods for saving plants from extinction is more relevant than ever before. More than 50% of the world's plant species are endemic to the 34 global biodiversity hotspots (GBHs), each holding at least 1,500 endemic plant species. In addition, a large number of small islands hold a number of endemic species on the brink of extinction. Con ...
DOI:
10.1007/s11627-010-9303-x

7. Conservation technologies for safeguarding and restoring threatened flora: case studies from Eastern Australia

Author:
Ashmore, Sarah E.; Hamilton, Kim N.; Offord, Catherine A.
Source:
In vitro cellular & developmental biology 2011 v.47 no.1 pp. 99-109
ISSN:
1054-5476
Subject:
Citrus; Orchidaceae; Syzygium; case studies; cryopreservation; endangered species; epiphytes; ferns and fern allies; flora; germination; indigenous species; plums; rain forests; seed storage; Australia
Abstract:
... This paper highlights recent advances and improved scientific understanding of conservation technologies through selected case studies on threatened plant species indigenous to Eastern Australia. This includes investigations into seed desiccation, storage responses and cryopreservation in rainforest species, particularly the socio-economically important Australian native Citrus spp., Davidsonia sp ...
DOI:
10.1007/s11627-010-9320-9

8. Cryopreservation of threatened native Australian species--what have we learned and where to from here

Author:
Kaczmarczyk, Anja; Turner, Shane R.; Bunn, Eric; Mancera, Ricardo L.; Dixon, Kingsley W.
Source:
In vitro cellular & developmental biology 2011 v.47 no.1 pp. 17-25
ISSN:
1054-5476
Subject:
botanical gardens; case studies; cell membranes; cryopreservation; genotype; germplasm conservation; guidelines; indigenous species; models; nitrogen; temperature; Australia
Abstract:
... Cryogenic storage techniques have been developed and adopted for more than 100 (mainly agricultural) plant species worldwide, and within Australia, at least 30 critically endangered plants have been stored long term using cryogenic approaches. Nevertheless, there are many species that are very difficult to store using current procedures, and organizations involved in plant germplasm conservation ( ...
DOI:
10.1007/s11627-010-9318-3

9. Infertility in a beef bull due to a failure in the capacitation process

Author:
C. Lessard; L.G. Siqueira; O. D'Amours; R. Sullivan; P. Leclerc; C. Palmer
Source:
Theriogenology 2011 v.76 no.5 pp. 891-899
ISSN:
0093-691X
Subject:
DNA; Red Angus; acrosome reaction; beef bulls; blastocyst; calcium; case studies; cryopreservation; heifers; in vitro fertilization; parthenogenesis; proteins; semen; sires; sperm concentration; sperm motility; spermatozoa; thawing
Abstract:
... The objective of this case report was to identify the cause of apparent idiopathic infertility in a Red Angus (beef) bull. Semen was collected by electroejaculation and submitted to a series of assays, including evaluation of sperm motility by computer-assisted sperm analysis (CASA), sperm morphology and DNA integrity, semen cryopreservation, AI, IVF, induction of the acrosome reaction, and determ ...
DOI:
10.1016/j.theriogenology.2011.04.020
PubMed:
21705058

10. Storage stability using cryopreservation: a case study in papaya

Author:
Ashmore, S.E.; Drew, R.A.; Kaity, A.
Source:
Acta horticulturae 2011 v. no.918 pp. 125-130
ISSN:
0567-7572
Subject:
Carica papaya; DNA fingerprinting; DNA methylation; adenosine monophosphate; case studies; cryopreservation; dormancy breaking; field experimentation; flowering; genetic stability; genetic variation; genotype; germination; plant morphology; seed storage; seeds; shoots; storage quality; storage temperature; wild relatives
Abstract:
... Ex situ conservation of Carica papaya and its crop wild relatives has been the subject of ten years of research in our laboratory. This paper summarises the achievements and key findings of this work. Both clonal and seed materials have been investigated to allow storage of elite clonal material as well as maximum genetic diversity. Clonal material from several genotypes has been successfully stor ...

11. Cryo-tolerance of zygotic embryos from recalcitrant seeds in relation to oxidative stress—A case study on two amaryllid species

Author:
Sershen; Boby Varghese; N.W. Pammenter; Patricia Berjak
Source:
Journal of plant physiology 2012 v.169 no.10 pp. 999-1011
ISSN:
0176-1617
Subject:
Amaryllis belladonna; Atropa belladonna; Haemanthus; antioxidant activity; antioxidants; case studies; cryopreservation; drying; enzyme activity; enzymes; glycerol; lipid peroxidation; oxidative stress; plant tissues; seeds; sucrose; thiobarbituric acid-reactive substances; viability
Abstract:
... Oxidative stress is a major component of cryoinjury in plant tissues. This study investigated the ability of recalcitrant (i.e. desiccation sensitive) Amaryllis belladonna L. and Haemanthus montanus Baker zygotic embryos to survive cryopreservation, in relation to oxidative stress. The study also investigated whether glycerol cryoprotection promoted embryo post-cryo survival by protecting enzymic ...
DOI:
10.1016/j.jplph.2012.02.016
PubMed:
22591858

12. In vitro storage and cryopreservation as substantial complements in concerted actions to better maintain and use crop germplasm

Author:
Keller, E.R.J.; Panis, B.; Engelmann, F.
Source:
Acta horticulturae 2012 v. no.961 pp. 35-50
ISSN:
0567-7572
Subject:
medicinal plants; European Union; potatoes; environmental hazards; in vitro culture; case studies; germplasm; genetic improvement; Lamiaceae; pests; biodiversity; cryopreservation; nitrogen; breeding; Musa; technology transfer; Allium; seed storage; bananas; France; Belgium
Abstract:
... The existence of modern cultivated plants is a result of interplaying modification by breeding and maintenance of valuable characters by storing the plant biodiversity. Genebanks were established to maintain and provide the crop diversity for use on the long term. Therefore, all possible methods for maintaining plant germplasm are necessary in order to fulfil this purpose for the various types of ...
DOI:
10.17660/ActaHortic.2012.961.2

13. 052 Considerations for development of GMP Biopreservation Solutions for Regenerative Medicine

Author:
Aby J Mathew
Source:
Cryobiology 2013 v.67 no.3 pp. 412
ISSN:
0011-2240
Subject:
biopreservation; blood serum; bone marrow; case studies; cryopreservation; good manufacturing practices; manufacturing; medicine; risk; transportation
Abstract:
... Cellular therapies and regenerative medicine utilize cell and tissue products sourced from blood, bone marrow, and various tissues. The clinical and commercial success of these products is potentially impacted by stability limitations, which include transport of the source material and biopreservation of the final cell or tissue product (either frozen or non-frozen). Traditional isotonic/extracell ...
DOI:
10.1016/j.cryobiol.2013.09.058

14. 116 Applications of the ΔtBIG method of cooling rate quantification to cell therapy cryopreservation under GMP compliance

Author:
David Frey; Jung Lee
Source:
Cryobiology 2013 v.67 no.3 pp. 431
ISSN:
0011-2240
Subject:
case studies; compliance; containers; cooling; cryopreservation; engineering; freezers; freezing; good manufacturing practices; ice; manufacturing; models; monitoring; neutropenia; stem cells; storage time; therapeutics
Abstract:
... Cryopreservation is a commonly adopted method for preserving allogeneic cell therapies through long-term storage and distribution to end-users. For clinical and eventually commercial manufacturing of these products, the cooling leg of the cryopreservation cycle is performed within a manufacturing environment that is regulated for Good Manufacturing Practices (GMP). To comply with GMP, the cooling ...
DOI:
10.1016/j.cryobiol.2013.09.122

15. Implementation and cost analysis of a regional farm animal cryobank: an Italian case study

Author:
Flavia Pizzi; Federica Turri; Teresa M. Gliozzi; Gustavo Gandini
Source:
Italian journal of animal science 2016 v.15 no.2 pp. 207-210
ISSN:
1828-051X
Subject:
animal science; blood; case studies; cattle; cost analysis; cryopreservation; genes; goats; semen; sheep
Abstract:
... Scientific and technical reports on farm animal genetic resources (AnGR) cryoconservation activities, and related costs, are needed to optimise conservation programmes. In this paper, we presented the recent Italian AnGR gene banking development, including the creation of the first regional animal cryobank, the ‘Lombardia Farm Animal Genetic Resources Cryobank’ (LABank). In order to provide indica ...
DOI:
10.1080/1828051X.2016.1153406

16. Open encapsulation-vitrification for cryopreservation of algae

Author:
Namrata Kumari; Mukesh Kumar Gupta; Raghubansh Kumar Singh
Source:
Cryobiology 2016 v.73 no.2 pp. 232-239
ISSN:
0011-2240
Subject:
Anabaena; Oocystis; algae; beta-mercaptoethanol; case studies; cost effectiveness; cryopreservation; genetic resources; glutathione; microorganisms; viability; vitrification
Abstract:
... Vitrification offers a cost-effective solution for the preservation and management of genetic resources with, low-cost international movement of selected genetic materials and for long-term maintenance of stable stocks of a wide variety of microorganisms. However, its success is limited by the wide range of algal species. Here, we report a simple open encapsulation-vitrification protocol of cryopr ...
DOI:
10.1016/j.cryobiol.2016.07.005

17. Physiological aspects of seed recalcitrance: a case study on the tree Aesculus hippocastanum

Author:
Natalie Obroucheva; Irina Sinkevich; Snejana Lityagina
Source:
Tree physiology 2016 v.36 no.9 pp. 1127-1150
ISSN:
1758-4469
Subject:
Aesculus hippocastanum; air; antioxidant activity; body water; carbohydrate metabolism; case studies; cryopreservation; dormancy; germination; hormonal regulation; longevity; recalcitrant seeds; tree physiology; trees
Abstract:
... Recalcitrant seeds are typical of some tropical and subtropical trees. Their post-shedding life activity proceeds in humid air and wet litter. They are desiccation sensitive and, for this reason, have a short life span and need some special procedures for cryopreservation. This review is devoted to the post-shedding life strategy of recalcitrant seeds, which includes the maintenance of high hydrat ...
DOI:
10.1093/treephys/tpw037

18. Recent aspects of Allium cryopreservation in the federal German genebank

Author:
E. R. J. Keller; A. Senula
Source:
Acta horticulturae 2016 no.1143 pp. 35-44
ISSN:
0567-7572
Subject:
Allium; antibiotics; bulbs; case studies; cloves; cryopreservation; differential scanning calorimetry; endophytes; explants; garlic; gene banks; inflorescences; microbial load; nitrogen; plant development; pollen; shoot tips; water content
Abstract:
... The genus Allium has been one of the main research objects of the Gatersleben institute for many years. Due to the high proportion of accessions that had been collected from the original sites a comprehensive living collection was established. Maintenance of living material was performed with a spectrum of methods, namely field culture, seed and in vitro storage, and cryopreservation. Vegetatively ...
DOI:
10.17660/ActaHortic.2016.1143.6

19. Cryopreservation of Proteins Using Ionic Liquids: A Case Study of Cytochrome c

Author:
Takekiyo Takahiro; Ishikawa Yuka; Yoshimura Yukihiro
Source:
Journal of physical chemistry 2017 v.121 no.32 pp. 7614-7620
ISSN:
1520-5207
Subject:
Fourier transform infrared spectroscopy; aqueous solutions; case studies; cattle; circular dichroism spectroscopy; cryopreservation; cryoprotectants; cytochrome c; denaturation; dialysis; dimethyl disulfide; dimethyl sulfoxide; heart; ionic liquids; nitrates; solubilization; thiocyanates; ultraviolet-visible spectroscopy
Abstract:
... Aqueous ionic liquid (IL) solutions form a glassy state at 77 K over a wide concentration of ILs. They have potential as novel cryopreservation/refolding solvents for proteins. However, even if proteins in glass-forming concentrations of ILs are preserved at 77 K, the recovery of activity and the structure of the proteins after cryopreservation are still unclear. To achieve high recovery of protei ...
DOI:
10.1021/acs.jpcb.7b05158

20. Automated image processing as an analytical tool in cell cryopreservation for bioprocess development

Author:
Sarah Gretzinger; Stefanie Limbrunner; Jürgen Hubbuch
Source:
Bioprocess and biosystems engineering 2019 v.42 no.5 pp. 665-675
ISSN:
1615-7591
Subject:
automation; biological specimen banks; biomass; biopharmaceuticals; bioprocessing; case studies; cell lines; cryopreservation; freeze-thaw cycles; freezing; image analysis; islets of Langerhans; models; storage temperature; thawing; therapeutics
Abstract:
... The continuous availability of cells with defined cell characteristics represents a crucial issue in the biopharmaceutical and cell therapy industry. Here, development of cell banks with a long-term stability is essential and ensured by a cryopreservation strategy. The strategy needs to be optimized for each cell application individually and usually comprises controlled freezing, storage at ultra- ...
DOI:
10.1007/s00449-019-02071-3

21. Conservation strategy for aquatic plants: endangered Ottelia acuminata (Hydrocharitaceae) as a case study

Author:
Jian-Ling Guo; Yan-Hong Yu; Jian-Wen Zhang; Zhi-Min Li; Yong-Hong Zhang; Sergei Volis
Source:
Biodiversity and conservation 2019 v.28 no.6 pp. 1533-1548
ISSN:
0960-3115
Subject:
Ottelia; amplified fragment length polymorphism; aquatic plants; case studies; climate; conservation areas; cryopreservation; environmental degradation; gene flow; genetic analysis; genetic similarity; genetic variation; geographical distribution; lakes; models; niches; phenotype; ponds; rivers; seeds; threatened species
Abstract:
... Knowledge of the extent and structure of genetic diversity and a species’ ecological niche are integral to formulating management plans for the conservation of threatened species. This is particularly important for aquatic organisms, which are especially vulnerable to human-induced environmental degradation and experience rapid global decline. To develop a proper conservation strategy for a threat ...
DOI:
10.1007/s10531-019-01740-9

22. Managing plant genetic resources using low and ultra-low temperature storage: a case study of tomato

Author:
Dariusz Kulus
Source:
Biodiversity and conservation 2019 v.28 no.5 pp. 1003-1027
ISSN:
0960-3115
Subject:
alleles; biochemical pathways; case studies; cooling; cryopreservation; cultivars; fecundity; freezers; frost; gene banks; genetic drift; genetic erosion; humidity; liquids; markets; nitrogen; plant genetic resources; pollen; protocols; seeds; shoot tips; somaclonal variation; storage time; temperature; tissues; tomatoes; vegetable crops; vitrification
Abstract:
... Ex situ preservation of plant genetic resources is essential. Tomato is one of the most important vegetable crops on the market. However, the genetic diversity of the clade is limited and suffering from genetic erosion phenomenon. Genebanks experience alleles loss on regeneration of small samples, genetic drift, and somaclonal variation in in vitro cultures. Therefore, the development of more effi ...
DOI:
10.1007/s10531-019-01710-1

23. Root cryopreservation to biobank medicinal plants: a case study for Hypericum perforatum L.

Author:
XuXu Yang; Elena Popova; Mukund R. Shukla; Praveen K. Saxena
Source:
In vitro cellular & developmental biology 2019 v.55 no.4 pp. 392-402
ISSN:
1054-5476
Subject:
Hypericum perforatum; aluminum foil; case studies; cryopreservation; genotype; gibberellic acid; glycerol; in vitro studies; medicinal plants; plant age; regrowth; root tips; sucrose; vitrification
Abstract:
... In this study, an effective root-based cryopreservation method was developed for Hypericum perforatum L., an important medicinal species, using in vitro plants. A systematic approach was applied to determine effective combinations of protocol steps such as preculture, osmoprotection, vitrification solution treatment, and unloading, followed by protocol optimization using a single-factor approach. ...
DOI:
10.1007/s11627-019-09999-x

24. Successful pregnancy following the transfer of re-vitrified twice-warmed embryos due to the forced cancellation of the primary FET: A case report

Author:
Maria Cristina Budani; Gian Mario Tiboni
Source:
Cryobiology 2020 v.97 pp. 242-244
ISSN:
0011-2240
Subject:
blastomeres; case studies; cryobiology; cryopreservation; embryo transfer; females; in vitro fertilization; patients; pregnancy
Abstract:
... Embryo cryopreservation represents a central procedure in in-vitro fertilization (IVF) programs. This report documents a Case of a successful pregnancy following the replacement of embryos that had to be re-vitrified due to the forced cancellation of the frozen embryo-transfer (FET).The 37- year-old patient was referred to our Assisted Reproductive Technology (ART) unit for idiopathic infertility ...
DOI:
10.1016/j.cryobiol.2020.08.009

25. An ethical analysis of cloning for genetic rescue: Case study of the black-footed ferret

Author:
Ronald L. Sandler; Lisa Moses; Samantha M. Wisely
Source:
Biological conservation 2021 v.257 pp. 109118
ISSN:
0006-3207
Subject:
Mustela nigripes; case studies; cryopreservation; endangered species; ethics; ferrets; fibroblasts; gene pool; genetic load; genetic rescue; genome
Abstract:
... Genetic rescue is a management tool used to decrease genetic load and increase fitness. The technique relies on the addition of unique genomes into a population's gene pool, but when no living candidates are available for translocation, preserved genetic resources may be an option. Biobanks are a source of cryopreserved material, including fibroblasts which have the potential to be used as source ...
DOI:
10.1016/j.biocon.2021.109118

26. Assessment of Vegetative Growth and Genetic Integrity of Vanilla planifolia Regenerants after Cryopreservation

Author:
María Teresa González-Arnao; Carlos A. Cruz-Cruz; Fabiola Hernández-Ramírez; Jorge A. Alejandre-Rosas; Ana Constanza Hernández-Romero
Source:
Plants 2022 v.11 no.13 pp. -
ISSN:
2223-7747
Subject:
Vanilla planifolia; case studies; cryopreservation; electrophoresis; genetic stability; greenhouses; leaves; microsatellite repeats; vegetative growth
Abstract:
... Vanilla planifolia Jacks. ex Andrews is the vanilla species with the most commercial and greatest economic importance. It has been used as a case study in different cryopreservation studies that involve three vitrification-based approaches: droplet-vitrification (D-V), V-cryoplate (V-Cp) and D-cryoplate (D-Cp). The aim of this study was to compare the impact of these cryogenic techniques on vegeta ...
DOI:
10.3390/plants11131630

27. Fish eDNA detection and its technical optimization: A case study of Acanthopagrus latus

Author:
Meijing Sun; Yu Guo; Naiqian Zhao; Shuo Zhang; Kun Pei; Chuanxin Qin
Source:
Marine environmental research 2022 v.176 pp. 105588
ISSN:
0141-1136
Subject:
Acanthopagrus; case studies; cryopreservation; environmental DNA; ethanol; fish; genes; research
Abstract:
... The development of a standardized eDNA detection process is the primary step in improving the accuracy and efficiency of eDNA detection. In this study, primers and probes with high specificity were selected to identify the COI gene of Acanthopagrus latus. Through experiments on the influence of different water quantities, methods of water sample preservation and water bathing times on the result o ...
DOI:
10.1016/j.marenvres.2022.105588