U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject cycloheximide Remove constraint Subject: cycloheximide
Start Over

Search

837  Search Results

« Previous | 1 - 50 of 837 |
Number of results to display per page

Search Results

1. AUF1 protects against ferroptosis to alleviate sepsis-induced acute lung injury by regulating NRF2 and ATF3

Author:
Yichun Wang; Diyu Chen; Han Xie; Mingwang Jia; Xiaofang Sun; Fang Peng; Feifei Guo; Daolin Tang
Source:
Cellular and molecular life sciences 2022 v.79 no.5 pp. 228
ISSN:
1420-682X
Subject:
cecum; cell viability; cycloheximide; epithelium; ferroptosis; glutathione; lipid peroxidation; lungs; models; precipitin tests; protein degradation; septic shock; survival rate; transcription factors; ubiquitin-protein ligase; ubiquitination
Abstract:
... BACKGROUND: The AU-rich element (ARE)-binding factor 1 (AUF1) acts as a switch for septic shock, although its underlying mechanisms remain largely unknown. In this study, we examined the biological significance and potential molecular mechanism of AUF1 in regulating ferroptosis in sepsis-induced acute lung injury (ALI). METHODS: Alveolar epithelial cells (AECs) challenged with ferroptosis-inducing ...
DOI:
10.1007/s00018-022-04248-8

2. Acquired resistance to severe ethanol stress-induced inhibition of proteasomal proteolysis in Saccharomyces cerevisiae

Author:
Vo Thi Anh Nguyet; Noboru Furutani; Ryoko Ando; Shingo Izawa
Source:
Biochimica et biophysica acta 2022 v.1866 no.12 pp. 130241
ISSN:
0304-4165
Subject:
Saccharomyces cerevisiae; alcoholic fermentation; cycloheximide; ethanol; proteasome endopeptidase complex; protein degradation; protein synthesis; proteolysis; yeasts
Abstract:
... Although the budding yeast, Saccharomyces cerevisiae, produces ethanol via alcoholic fermentation, high-concentration ethanol is harmful to yeast cells. Severe ethanol stress (> 9% v/v) inhibits protein synthesis and increases the level of intracellular protein aggregates. However, its effect on proteolysis in yeast cells remains largely unknown. We examined the effects of ethanol on proteasomal p ...
DOI:
10.1016/j.bbagen.2022.130241

3. Analysis of cycloheximide in rat specimens using liquid chromatography with tandem mass spectrometry detection

Author:
Hamdah M. Al Nebaihi; Tyson S. Le; Neal M. Davies; Dion R. Brocks
Source:
Journal of chromatography 2022 v.1190 pp. 123112
ISSN:
1570-0232
Subject:
ambient temperature; ammonium acetate; colchicine; cycloheximide; hexane; high performance liquid chromatography; intravenous injection; isopropyl alcohol; methylene chloride; rats; tandem mass spectrometry
Abstract:
... The development of a selective and sensitive high-performance liquid chromatographic tandem mass spectrometric method for the determination of cycloheximide (CHX) in rat blood and plasma is described. The extraction of CHX and colchicine as internal standard from blood fluid (0.1 mL) was achieved using n-hexane: dichloromethane: isopropanol (20:10:1 v/v/v). The mobile phase, a combination of metha ...
DOI:
10.1016/j.jchromb.2022.123112

4. Analysis of seed-associated bacteria and fungi on staple crops using the cultivation and metagenomic approaches

Author:
Valerija Tkalec; Aleksander Mahnic; Peter Gselman; Maja Rupnik
Source:
Folia microbiologica 2022 v.67 no.3 pp. 351-361
ISSN:
0015-5632
Subject:
Aspergillus; Bacteroides; Curtobacterium; Dermateaceae; Paenibacillus; Pantoea; agar; barley; corn; cycloheximide; fungi; metagenomics; microbial load; microbiome; nystatin; seed quality; wheat
Abstract:
... One of the key factors affecting seed quality is microbial communities residing on and in the seeds. In this study, microbial populations of seeds of conventionally and organically produced wheat, barley, and maize were analyzed using two different approaches: the cultivation method and metagenomics. For cultivation, three basic media were used: DG18 (for fungi), and nutrient agar or tryptic soy a ...
DOI:
10.1007/s12223-022-00958-5

5. Assessing the efficacy of antibiotic treatment to produce earthworms with a suppressed microbiome

Author:
Henny O. Omosigho; Elmer Swart; Tom P. Sizmur; Dave J. Spurgeon; Claus Svendsen; Liz J. Shaw
Source:
European journal of soil biology 2022 v.108 pp. 103366
ISSN:
1164-5563
Subject:
Aeromonas; Allolobophora chlorotica; Comamonas; Eisenia fetida; Lumbricus terrestris; Sphingobacterium; agar; ampicillin; ciprofloxacin; community structure; culture media; cycloheximide; earthworms; gentamicin; microbiome; nalidixic acid; organic matter; sequence analysis; soil
Abstract:
... Earthworms are an integral part of soil ecosystems, especially for their role in soil functions such as organic matter (OM) decomposition and nutrient cycling. Earthworms and microorganisms are interdependent, and a considerable portion of the contribution earthworms make to influencing OM fate is through interactions with microorganisms. However, the importance of the earthworm-associated microbi ...
DOI:
10.1016/j.ejsobi.2021.103366

6. The BRCA2 missense mutation K2497R suppressed self-degradation and increased ATP production and cell proliferation

Author:
Gerelmaa Enkhbat; Akira Nakanishi; Yoshio Miki
Source:
Biochemical and biophysical research communications 2022 v.590 pp. 27-33
ISSN:
0006-291X
Subject:
breast neoplasms; cell proliferation; centrosomes; cycloheximide; cytokinesis; genes; heat shock proteins; interphase; lysine; missense mutation; research; tumor suppressor proteins; ubiquitination
Abstract:
... Breast cancer susceptibility gene 2 (BRCA2) mediates genome maintenance during the S phase of the cell cycle, with important roles in replication stress, centrosome replication, and cytokinesis. In this study, we showed that a small heat shock protein, HSP27, interacted with and participated in the degradation of BRCA2 in estrogen-treated MCF-7 cells. BRCA2 degradation reportedly requires ubiquiti ...
DOI:
10.1016/j.bbrc.2021.12.073

7. Candidiasis in Birds (Galliformes, Anseriformes, Psittaciformes, Passeriformes, and Columbiformes): A Focus on Antifungal Susceptibility Pattern of Candida albicans and Non-albicans Isolates in Avian Clinical Specimens

Author:
Forough Talazadeh; Masoud Ghorbanpoor; Abolfazl Shahriyari
Source:
Topics in companion animal medicine 2022 v.46 pp. 100598
ISSN:
1938-9736
Subject:
Anseriformes; Candida albicans; Columbiformes; Galliformes; Passeriformes; Psittaciformes; agar; amphotericin B; antibiotic resistance; candidiasis; chloramphenicol; cloaca; cycloheximide; cytochrome P-450; disk diffusion antimicrobial test; fluconazole; fungi; genes; glucose; itraconazole; ketoconazole; medicine; microscopy; nystatin; oligodeoxyribonucleotides; pets; pharynx; Iran
Abstract:
... Candidiasis is a fungal infection caused by Candida species which has been reported in most domestic and wild birds and mammals. In this study, 196 samples from different species of birds with suspected symptoms of candidiasis were examined. Pharyngeal swabs, cloacal swabs, and fecal samples were taken from the birds. The samples were cultured in sabouraud dextrose agar (SDA) containing cyclohexim ...
DOI:
10.1016/j.tcam.2021.100598

8. Comparison of methods for isolating entomopathogenic fungi from soil samples

Author:
Correa Thais A.; Santos Fernanda S.; Camargo Mariana G.; Quinelato Simone; Bittencourt Vânia R. E. P.; Golo Patricia S.
Source:
Journal of visualized experiments 2022 no.179 pp. e63353
ISSN:
1940-087X
Subject:
Beauveria; Galleria mellonella; Metarhizium; Tenebrio molitor; arthropod pests; arthropods; biobased products; biodiversity; chloramphenicol; cycloheximide; habitats; insects; markets; pest control; selective media; soil; thiabendazole; yeast extract
Abstract:
... The goal of the present study is to compare the effectiveness of using insect baits versus artificial selective medium for isolating entomopathogenic fungi (EPF) from soil samples. The soil is a rich habitat for microorganisms, including EPF particularly belonging to the genera Metarhizium and Beauveria, which can regulate arthropod pests. Biological products based on fungi are available in the ma ...
DOI:
10.3791/63353

9. Danhe granule ameliorates nonalcoholic steatohepatitis and fibrosis in rats by inhibiting ceramide de novo synthesis related to CerS6 and CerK

Author:
Meixia Zhu; Zhixin Jia; Xiaoning Yan; Lirong Liu; Cong Fang; Menghan Feng; Yihang Dai; Yinhuan Zhang; Hao Wu; Beibei Huang; Yueting Li; Jie Liu; Hongbin Xiao
Source:
Journal of ethnopharmacology 2022 v.295 pp. 115427
ISSN:
0378-8741
Subject:
animal models; blood serum; blood stasis; body weight; ceramide glucosyltransferase; ceramides; cycloheximide; diet; enzyme activity; fatty liver; fibrosis; free fatty acids; inflammation; interleukin-6; liver; mechanism of action; non-specific serine/threonine protein kinase; oils; protein synthesis; quantitative polymerase chain reaction; serine; sphingosine N-acyltransferase; traditional medicine; triacylglycerols
Abstract:
... Danhe granule (DHG) is used by Chinese doctors to treat blood stasis, phlegm and dampness. Its lipid-lowering ability has been investigated in our previous research. However, the anti-liver inflammatory and fibrotic effects and mechanism of action of DHG in non-alcoholic steatohepatitis (NASH) have not been explored. To evaluate the ameliorative effects of DHG on liver inflammation and fibrosis in ...
DOI:
10.1016/j.jep.2022.115427

10. Detection and prevalence of Rosellinia necatrix in South African avocado orchards

Author:
Jesse Hartley; Juanita Engelbrecht; Noëlani van den Berg
Source:
European journal of plant pathology 2022 v.163 no.4 pp. 961-978
ISSN:
0929-1873
Subject:
Rosellinia necatrix; avocados; chlorothalonil; cycloheximide; forestry; host plants; nystatin; pathogens; plant pathology; protocols; root rot; selective media; soil; South Africa
Abstract:
... Rosellinia necatrix is an ascomycete that causes white root rot (WRR) of several plant host species resulting in economic losses to affected agricultural and forestry industries in various regions. This study aimed to identify and monitor the prevalence of R. necatrix in avocado orchards in South Africa. We used both morphological and molecular methods to isolate and identify R. necatrix from dise ...
DOI:
10.1007/s10658-022-02532-8

11. EdU sensing: The Raman way of following endothelial cell proliferation in vitro and ex vivo

Author:
Basseem Radwan; Stefano Rocchetti; Ewelina Matuszyk; Magdalena Sternak; Maciej Stodulski; Robert Pawlowski; Jacek Mlynarski; Krzysztof Brzozowski; Stefan Chlopicki; Malgorzata Baranska
Source:
Biosensors & bioelectronics 2022 v.216 pp. 114624
ISSN:
0956-5663
Subject:
DNA; DNA replication; aorta; azides; biosensors; cell proliferation; cycloheximide; doxorubicin; endothelial cells; fluorescence; homeostasis; mice; permeability
Abstract:
... Endothelial cells line the lumen of all vessels in the body and maintain vascular homeostasis. In particular, endothelial cell regeneration in response to insult sustain functional endothelial layer. EdU (5-ethynyl-2′-deoxyuridine) is an alkyne-tagged proliferation probe that incorporates into newly synthesized DNA and is used for fluorescence imaging of cell proliferation with the use of “click c ...
DOI:
10.1016/j.bios.2022.114624

12. Efflux Pumps and Multidrug-Resistance in Pyricularia oryzae Triticum Lineage

Author:
Samara Nunes Campos Vicentini; Silvino Intra Moreira; Abimael Gomes da Silva; Tamiris Yoshie Kiyama de Oliveira; Tatiane Carla Silva; Fabio Gomes Assis Junior; Loane Dantas Krug; Adriano Augusto de Paiva Custódio; Rui Pereira Leite Júnior; Paulo Eduardo Teodoro; Bart Fraaije; Paulo Cezar Ceresini
Source:
Agronomy 2022 v.12 no.9 pp. -
ISSN:
2073-4395
Subject:
Magnaporthe oryzae; Triticum; agronomy; blast disease; chlorides; cycloheximide; gene expression regulation; multiple drug resistance; pathogens; rhodamines; transcriptomics; transporters
Abstract:
... Widespread resistance to QoIs, DMI and SDHIs fungicides has been reported for Brazilian populations of the wheat blast pathogen Pyricularia oryzae Triticum lineage (PoTl). A pre-existing resistance mechanism not associated with target site mutations has been indicated for resistance to DMIs and SDHIs, with strong indication that PoTl has multidrugresistance (MDR). Therefore, the main objective of ...
DOI:
10.3390/agronomy12092068

13. First Report of Laurel Wilt Disease Caused by Raffaelea lauricola on Sassafras in Virginia

Author:
R. Gazis; K. M. DeWitt; L. K. Johnson; L. A. Chamberlin; A. H. Kennedy; M. A. Hansen; E. A. Bush
Source:
Plant disease 2022 v.106 no.6 pp. 1763
ISSN:
0191-2917
Subject:
Lindera benzoin; Papilio troilus; Persea borbonia; Raffaelea lauricola; Sassafras albidum; Xyleborus glabratus; blastospores; butterflies; conidia; conidiophores; culture media; cycloheximide; dieback; discoloration; fungi; laurel wilt; leaves; mortality; pathogenicity; roadsides; sapwood; soil; streptomycin; symbionts; turgor; vascular wilt; Canada; Georgia; Northeastern United States; Tennessee; Virginia
Abstract:
... Laurel wilt is a lethal vascular disease affecting native Lauraceae in North America. The causal fungus, Raffaelea lauricola T.C. Harr., Fraedrich & Aghayeva, and its symbiont, redbay ambrosia beetle, Xyleborus glabratus Eichhoff, are native to Asia (Fraedrich et al. 2008; Harrington et al. 2008). Since their introduction near Savannah, Georgia, in 2002 (Fraedrich et al. 2008), laurel wilt has spr ...
DOI:
10.1094/PDIS-11-21-2616-PDN

14. Fungal dominance of gross nitrogen mineralisation in an acidic upland soil amended with organic fertiliser

Author:
Jing Wang; Ruibiao Fu; Yong Li; Xiaoshun Tu; Zhaoxiong Chen; Ahmed S. Elrys; Yi Cheng; Jinbo Zhang; Zucong Cai; Christoph Müller; Lifeng Ma
Source:
European journal of soil science 2022 v.73 no.4 pp. e13282
ISSN:
1351-0754
Subject:
acid soils; ammonium; carbon; cycloheximide; fungi; mineralization; nitrate reduction; nitrification; nitrogen; organic fertilizers; rapeseed cake; streptomycin; upland soils
Abstract:
... Fungi typically prevail in acidic soils and have a stronger preference for complex organic compounds than bacteria. Thus, we hypothesized that fungi would play a more important role in gross nitrogen (N) transformation in acidic soil than bacteria, and the dominance of fungi on gross N transformations in acidic soil would increase following complex organic inputs. Herein, we determined bacterial a ...
DOI:
10.1111/ejss.13282

15. Glabridin induces paraptosis-like cell death via ER stress in breast cancer cells

Author:
Xiang Cui; Min Cui
Source:
Heliyon 2022 v.8 no.9 pp. e10607
ISSN:
2405-8440
Subject:
Glycyrrhiza glabra; breast neoplasms; breasts; caspase-3; cycloheximide; dose response; endoplasmic reticulum; flavonoids; fluorescence; humans; membrane potential; mitochondria; mitochondrial membrane; programmed cell death; protein synthesis; reactive oxygen species; therapeutics; transmission electron microscopy; viability
Abstract:
... Glabridin, a polyphenolic flavonoid isolated from the root of the glycyrrhiza glabra, has been demonstrated to have anti-tumor properties in human malignancies. This study found that glabridin decreased the viability of human breast cancer MDA-MB-231 and MCF7 cells in a dose-dependent manner that was not involved in the caspase-3 cascade. Glabridin promoted the formation of extensive cytoplasmic v ...
DOI:
10.1016/j.heliyon.2022.e10607

16. Identification and utilization of a mutated 60S ribosomal subunit coding gene as an effective and cost-efficient selection marker for Tetrahymena genetic manipulation

Author:
Yu Qiao; Ting Cheng; Jiachen Zhang; Saleh A. Alfarraj; Miao Tian; Yifan Liu; Shan Gao
Source:
International journal of biological macromolecules 2022 v.204 pp. 1-8
ISSN:
0141-8130
Subject:
Tetrahymena thermophila; cost effectiveness; cycloheximide; eukaryotic cells; genetic engineering; molecular biology; resistance genes
Abstract:
... Since the onset of molecular biology, the ciliate Tetrahymena thermophila has been one of the most convenient single-celled model eukaryotes for genetics, biochemistry, and cell biology. Particularly, thanks to the availability of several different selection markers, it is possible to knock out or knock in genes at multiple genetic loci simultaneously in Tetrahymena, which makes it an excellent mo ...
DOI:
10.1016/j.ijbiomac.2022.01.188

17. In vitro induction of catfish, Clarias batrachus, oocyte maturation by conspecific vitellogenin 1 (CFVg1)

Author:
Debapriya Bhattacharya; Shrabanti Sarkar; Panchanan Nath
Source:
Fish physiology and biochemistry 2022 v.48 no.1 pp. 227-239
ISSN:
0920-1742
Subject:
Clarias batrachus; actinomycin D; appetite; carp; catfish; conspecificity; cycloheximide; oocytes; theophylline; trypsin; vitellogenin
Abstract:
... Present study demonstrates that conspecific vitellogenin1 (CFVg1) induces oocyte maturation in the catfish, Clarias batrachus. CFVg1 is able to develop fertilizable eggs in the Clarias batrachus. Therefore, different in vitro oocyte culture experiments were designed to see whether CFVg1 has efficacy of oocyte maturation and its pathway. In in vitro oocyte culture experiment, CFVg1 showed a dose- a ...
DOI:
10.1007/s10695-022-01050-y

18. Molecular basis of cycloheximide resistance in the Ophiostomatales revealed

Author:
Brenda D. Wingfield; Mike J. Wingfield; Tuan A. Duong
Source:
Current genetics 2022 v.68 no.3-4 pp. 505-514
ISSN:
0172-8083
Subject:
Kluyveromyces; Ophiostomatales; antibiotics; cycloheximide; evolution; genes; glutamine; mutation; ribosomal proteins; yeasts
Abstract:
... Resistance to the antibiotic Cycloheximide has been reported for a number of fungal taxa. In particular, some yeasts are known to be highly resistant to this antibiotic. Early research showed that this resulted from a transition mutation in one of the 60S ribosomal protein genes. In addition to the yeasts, most genera and species in the Ophiostomatales are highly resistant to this antibiotic, whic ...
DOI:
10.1007/s00294-022-01235-1

19. Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus

Author:
Liyuan Yi; Caiyun Sun; Jinying Wu; Zengxin Hou; Wensheng Li
Source:
Journal of fish diseases 2022 v.45 no.6 pp. 833-846
ISSN:
0140-7775
Subject:
Oreochromis niloticus; Siniperca chuatsi; Streptococcus agalactiae; Takifugu rubripes; amino acids; apoptosis; cycloheximide; cytotoxicity; fish; gene expression; kidneys; leukocytes; liver; morphogenesis; neoplasm cells; neoplasms; polyinosinic-polycytidylic acid; recombinant proteins; spleen; tumor necrosis factors
Abstract:
... The tumour necrosis factor superfamily (TNFSF) plays critical roles in tumour apoptosis, tissue morphogenesis and lineage determination. TNFSF10 (TRAIL or Apol‐2) belongs to the tumour necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumour cell lines upon binding to death‐inducing signalling receptors. In this study, we identified TNFSF10 from Nile tilapia (O ...
DOI:
10.1111/jfd.13609

20. Novel Synergistic Anti-Enteroviral Drug Combinations

Author:
Aleksandr Ianevski; Eva Zusinaite; Tanel Tenson; Valentyn Oksenych; Wei Wang; Jan Egil Afset; Magnar Bjørås; Denis E. Kainov
Source:
Viruses 2022 v.14 no.9 pp. -
ISSN:
1999-4915
Subject:
Enterovirus; adenocarcinoma; cycloheximide; emetine; epithelium; humans
Abstract:
... Background: Enterovirus infections affect people around the world, causing a range of illnesses, from mild fevers to severe, potentially fatal conditions. There are no approved treatments for enterovirus infections. Methods: We have tested our library of broad-spectrum antiviral agents (BSAs) against echovirus 1 (EV1) in human adenocarcinoma alveolar basal epithelial A549 cells. We also tested com ...
DOI:
10.3390/v14091866

21. Sodium arsenite but not aluminum chloride stimulates ABC transporter activity in renal proximal tubules of killifish (Fundulus heteroclitus)

Author:
Goezde Oezen; Eva-Maria Schentarra; Jan Stephan Bolten; Joerg Huwyler; Gert Fricker
Source:
Aquatic toxicology 2022 v.252 pp. 106314
ISSN:
0166-445X
Subject:
Fundulus heteroclitus; P-glycoproteins; active transport; aluminum; aluminum chloride; arsenic; breast neoplasms; confocal microscopy; cycloheximide; endothelin receptors; exports; fluorescence; image analysis; mammals; models; peptides; phosphatidylinositol 3-kinase; pollutants; rapamycin; sodium arsenite; toxicology; urine; xenobiotics
Abstract:
... ABC export proteins including Multidrug resistance-related protein 2 (Mrp2) serve as detoxification mechanism in renal proximal tubules due to active transport of xenobiotics and metabolic waste products into primary urine. The environmental pollutants aluminum and arsenic interfere with a multitude of regulatory mechanisms in the body and here their impact on ABC transporter function was studied. ...
DOI:
10.1016/j.aquatox.2022.106314

22. Steroid modification by filamentous fungus Drechslera sp.: Focus on 7-hydroxylase and 17β-hydroxysteroid dehydrogenase activities

Author:
Vyacheslav Kollerov; Andrei Shutov; Alexey Kazantsev; Marina Donova
Source:
Fungal biology 2022 v.126 no.1 pp. 91-100
ISSN:
1878-6146
Subject:
Drechslera; androstanes; cycloheximide; fungi; hydroxylation; lithocholic acid; mass spectrometry; mycelium; mycology; nuclear magnetic resonance spectroscopy; oxidoreductases; prasterone; pregnanes; pregnenolone; testosterone
Abstract:
... Fungal strain Drechslera sp. Ph F-34 was shown to modify 3-oxo- and 3-hydroxy steroids of androstane series to form the corresponding allylic 7-alcohols and 17β-reduced derivatives thus evidencing the presence of 7α-, 7β-hydroxylase and 17β-hydroxysteroid dehydrogenase (17β-HSD) activities. The growing mycelium predominantly hydroxylated androsta-1,4-diene-3,17-dione (ADD) at the 7β-position, whil ...
DOI:
10.1016/j.funbio.2021.11.002

23. Synergy between fungi and bacteria promotes polycyclic aromatic hydrocarbon cometabolism in lignin-amended soil

Author:
Decheng Gu; Xingjia Xiang; Yucheng Wu; Jun Zeng; Xiangui Lin
Source:
Journal of hazardous materials 2022 v.425 pp. 127958
ISSN:
0304-3894
Subject:
Basidiomycota; Methylophilaceae; Sphingomonadaceae; anthracenes; biodegradation; bioremediation; carbon dioxide; cycloheximide; fungi; isotope labeling; lignin; mineralization; models; soil
Abstract:
... Lignin enhanced biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soil, but collaboration among soil microorganisms during this process remains poorly understood. Here we explored the relations between microbial communities and PAH transformation in soil microcosms amended with lignin. Mineralization of the four-ring benzo(a)anthracene (BaA), which was selected as a model, was determine ...
DOI:
10.1016/j.jhazmat.2021.127958

24. Temperature modulation of CAMTA3 gene induction activity is mediated through the DNA binding domain

Author:
Lumen Chao; Yongsig Kim; Sarah J. Gilmour; Michael F. Thomashow
Source:
plant journal 2022 v.112 no.1 pp. 235-248
ISSN:
0960-7412
Subject:
9-cis-epoxycarotenoid dioxygenase; Arabidopsis thaliana; DNA; acclimation; cold; cycloheximide; gene expression; gene induction; genes; models; mutants; temperature; transcription (genetics); transcriptional activation
Abstract:
... The calmodulin‐binding transcription activator (CAMTA) proteins of Arabidopsis thaliana play a major role in cold acclimation, contributing to the rapid induction of the C‐REPEAT BINDING FACTOR (CBF) genes and other genes that impart freezing tolerance in plants exposed to cold temperature (4°C). The goal of this study was to better understand how the gene induction activity of CAMTA3 is modulated ...
DOI:
10.1111/tpj.15944

25. The foodborne contaminant deoxynivalenol exacerbates DNA damage caused by a broad spectrum of genotoxic agents

Author:
Marion Garofalo; Delphine Payros; Eric Oswald; Jean-Philippe Nougayrède; Isabelle P. Oswald
Source:
Science of the total environment 2022 v.820 pp. 153280
ISSN:
0048-9697
Subject:
DNA damage; cisplatin; cycloheximide; deoxynivalenol; digestive system; environment; etoposide; food chain; genotoxicity; mutagens; phenotype; ribosomes
Abstract:
... Humans are exposed to different contaminants including mycotoxins. Deoxynivalenol (DON), a potent ribosome inhibitor, is a highly prevalent mycotoxin in the food chain worldwide. Although DON is not genotoxic, we previously showed that it exacerbates the genotoxicity of colibactin, a DNA-crosslinking toxin produced by bacteria in the gut. In the present study, we investigated whether this phenotyp ...
DOI:
10.1016/j.scitotenv.2022.153280

26. An in vitro bioassay for evaluating the virulence of Pseudomonas savastanoi pv savastanoi isolates on olive

Author:
Mazen Salman; Ali McClean; Daniel Kluepfel
Source:
Australasian plant disease notes 2022 v.17 no.1 pp. 1
ISSN:
1833-928X
Subject:
bioassays; cephalexin; cycloheximide; germplasm conservation; leaves; olive knot disease; olives; vancomycin; virulence
Abstract:
... Pseudomonas savastanoi pv. savastanoi causes olive knot disease that afflicts olive trees globally. A sensitive and reproducible in vitro pathogenicity bioassay using detached leaf segments was developed after isolation of Psav on KB medium amended with vancomycin, cephalexin and cycloheximide. Symptoms were clear under 20X magnification after 10 days. This bioassay will facilitate rapid screening ...
DOI:
10.1007/s13314-022-00448-0

27. The interaction of O-GlcNAc-modified NLRX1 and IKK-α modulates IL-1β expression in M1 macrophages

Author:
Liqiong Chen; Yueliang Li; Shuxian Zeng; Shujuan Duan; Zhuanglin Huang; Yi Liang
Source:
In vitro cellular & developmental biology 2022 v.58 no.5 pp. 408-418
ISSN:
1071-2690
Subject:
N-acetylglucosamine; confocal microscopy; cycloheximide; inflammation; macrophages; precipitin tests; transcription factor NF-kappa B; ubiquitination
Abstract:
... NOD-like receptor (NLR)X1 (NLRX1) is a negative regulator of inflammation by inhibiting nuclear factor-κB (NF-κB) signaling and downstream pro-inflammatory factors. However, its post-translational modification and how it participates in regulating the inflammatory responses in macrophages are still unclear. Here, we found that NLRX1 was modified with O-linked N-acetylglucosamine (O-GlcNAc). The in ...
DOI:
10.1007/s11626-022-00654-1

28. A premature stop codon in BrFLC2 transcript results in early flowering in oilseed-type Brassica rapa plants

Author:
Sujeong Kim; Jin A. Kim; Hajeong Kang; Dong-Hwan Kim
Source:
Plant molecular biology 2022 v.108 no.3 pp. 241-255
ISSN:
0167-4412
Subject:
Brassica rapa subsp. trilocularis; Chinese cabbage; chromosome mapping; chromosomes; cooling; cycloheximide; early flowering; exons; genomics; inbred lines; late flowering; molecular biology; single nucleotide polymorphism; stop codon; transcriptome; vernalization
Abstract:
... KEY MESSAGE: Nonsense-mediated mRNA decay (NMD)-mediated degradation of BrFLC2 transcripts is the main cause of rapid flowering of oilseed-type B. rapa ‘LP08’ plants. Many Brassica species require vernalization (long-term winter-like cooling) for transition to the reproductive stage. In the past several decades, scientific efforts have been made to discern the molecular mechanisms underlying verna ...
DOI:
10.1007/s11103-021-01231-y

29. 1 Effect of cytokine-supplemented maturation medium on bovine somatic cell nuclear transfer embryo development

Author:
J. Keim; Y. Liu; M. Regouski; R. Stott; G. N. Singina; I. A. Polejaeva
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 108
ISSN:
1031-3613
Subject:
blastocyst; bovine serum albumin; calcium; cattle; cycloheximide; embryo transfer; embryogenesis; estrus; fetal bovine serum; fibroblast growth factor 2; fibroblasts; humans; insulin-like growth factor I; leukemia inhibitory factor; magnesium; metaphase; oocytes; penicillins; pregnancy rate; somatic cells; sorbitol; streptomycin
Abstract:
... Invitro maturation is an important process in the invitro production of embryos. It has been shown recently that 3 cytokines: fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), increase the efficiency of IVM, blastocyst production, and invivo development following somatic cell nuclear transfer (SCNT) in pigs (Yuan et al. 2017 PNAS 114, E57 ...
DOI:
10.1071/RDv33n2Ab1

30. 133 Effect of cytokines on the quality bovine oocytes matured invitro

Author:
G. N. Singina; E. N. Shedova; T. E. Taradajnic
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 174-175
ISSN:
1031-3613
Subject:
blastocyst; cattle; cell cleavage; cumulus oophorus; cycloheximide; embryo culture; fetal bovine serum; fibroblast growth factor 2; follicle-stimulating hormone; in vitro fertilization; insulin-like growth factor I; ionophores; leukemia inhibitory factor; luteinizing hormone; oocytes; parthenogenesis; pyridines; sodium pyruvate
Abstract:
... As has been shown previously, fibroblast growth factor 2 (FGF2), leukaemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1) in combination play a positive role in maintaining the quality of mammalian oocytes maturing invitro. In the present work, we studied the effects of these cytokines in optimal concentrations when added in combination to IVM medium on the nuclear status and dev ...
DOI:
10.1071/RDv33n2Ab133

31. 5,7-Dimethoxyflavone enhances barrier function by increasing occludin and reducing claudin-2 in human intestinal Caco-2 cells

Author:
Yunika Mayangsari; Mayu Okudaira; Chinatsu Mano; Yuki Tanaka; Osamu Ueda; Tomohiro Sakuta; Yoshiharu Suzuki; Yoshinari Yamamoto; Takuya Suzuki
Source:
Journal of functional foods 2021 v.85 pp. 104641
ISSN:
1756-4646
Subject:
cycloheximide; cytoskeleton; dextran; electrical resistance; ginger; humans; inflammation; intestines; occludins; permeability; rapamycin; tight junctions; transcription (genetics)
Abstract:
... Defects in intestinal tight junction (TJ) barrier cause intestinal inflammation. We investigated the effects of 5,7-dimethoxyflavone (DMF), abundantly found in black ginger, on the TJ barrier in human intestinal Caco-2 cells. DMF reinforced TJ barrier integrity, indicated by increased transepithelial electrical resistance and reduced dextran permeability in Caco-2 cells. Immunoblot analysis reveal ...
DOI:
10.1016/j.jff.2021.104641

32. Apoptotic effect of compound K in hepatocellular carcinoma cells via inhibition of glycolysis and Akt/mTOR/c‐Myc signaling

Author:
Nari Shin; Hyo‐Jung Lee; Deok Yong Sim; Eunji Im; Ji Eon Park; Woon Yi Park; Ah Reum Cho; Bum Sang Shim; Sung‐Hoon Kim
Source:
Phytotherapy research 2021 v.35 no.7 pp. 3812-3820
ISSN:
0951-418X
Subject:
Panax; apoptosis; cycloheximide; cytotoxicity; glycolysis; hepatoma; hexokinase; humans; isozymes; metabolites; phytotherapy; proteinases; pyruvate kinase; pyruvic acid; rapamycin; research; saponins
Abstract:
... Since the AKT/mammalian target of rapamycin (mTOR)/c‐Myc signaling plays a pivotal role in the modulation of aerobic glycolysis and tumor growth, in the present study, the role of AKT/mTOR/c‐Myc signaling in the apoptotic effect of Compound K (CK), an active ginseng saponin metabolite, was explored in HepG2 and Huh7 human hepatocellular carcinoma cells (HCCs). Here, CK exerted significant cytotoxi ...
DOI:
10.1002/ptr.7087

33. Attenuation of cytosolic translation by RNA oxidation is involved in singlet oxygen‐mediated transcriptomic responses

Author:
Eugene Koh; Dekel Cohen; Alexander Brandis; Robert Fluhr
Source:
Plant, cell and environment 2021 v.44 no.11 pp. 3597-3615
ISSN:
0140-7791
Subject:
Arabidopsis; cycloheximide; cytoplasm; environment; genes; half life; jasmonic acid; oxidation; singlet oxygen; transcriptome; transcriptomics
Abstract:
... Singlet oxygen (¹O₂) production is associated with stress signalling. Here, using Arabidopsis as a model system, we study the effects of the accumulation of 8‐hydroxyguanosine (8‐oxoG), a major product of ¹O₂‐mediated RNA oxidation. We show that 8‐oxoG can accumulate in vivo when ¹O₂ is produced in the cytoplasm. Conditions for such production include the application of RB in the light, dark‐to‐li ...
DOI:
10.1111/pce.14162

34. Autoactivation of Translation Causes the Bloom of Prorocentrum donghaiense in Harmful Algal Blooms

Author:
Xin Cao; Zhong Guo; Hualong Wang; Yuelei Dong; Songhui Lu; Qing-Yu He; Xuesong Sun; Gong Zhang
Source:
Journal of proteome research 2021 v.20 no.6 pp. 3179-3187
ISSN:
1535-3907
Subject:
Prorocentrum; cycloheximide; ecosystems; energy; genome; genomics; human health; poisonous algae; proteome; research
Abstract:
... Harmful algal blooms (HABs) are symptomatic of ecosystem imbalance, leading to major worldwide marine natural disasters, and seriously threaten the human health. Some HAB algae’s exceptional genome size prohibited the genomic investigations on molecular mechanisms, for example, Prorocentrum. This study performed translatome sequencing (RNC-seq) for Prorocentrum donghaiense to assemble the translat ...
DOI:
10.1021/acs.jproteome.1c00051

35. Bibenzyl analogue DS-1 inhibits MDM2-mediated p53 degradation and sensitizes apoptosis in lung cancer cells

Author:
Hardyanti Eka Putri; Bodee Nutho; Thanyada Rungrotmongkol; Boonchoo Sritularak; Chanida Vinayanuwattikun; Pithi Chanvorachote
Source:
Phytomedicine 2021 v.85 pp. 153534
ISSN:
0944-7113
Subject:
Western blotting; apoptosis; cancer therapy; cisplatin; computer simulation; cycloheximide; cytotoxicity; lung neoplasms; phosphorylation; precipitin tests; van der Waals forces
Abstract:
... Lung cancer is a leading fatal malignancy due to the high incidence of treatment failure. Dysfunction of the tumor suppressor p53 contributes to cancer initiation, progression, and therapeutic resistance. Targeting MDM2, a negative regulator of p53, has recently attracted interest in cancer drug research as it may restore tumor suppressive function.The present study aimed to investigate the effect ...
DOI:
10.1016/j.phymed.2021.153534

36. Biocides provide a source of carbon and nitrogen directly to surviving microbes and indirectly through a pulse in microbial necromass

Author:
Mohammad Rahmat Ullah; Yolima Carrillo; Feike A. Dijkstra
Source:
Applied soil ecology 2021 v.160 pp. 103862
ISSN:
0929-1393
Subject:
biocides; captan; carbon; carbon dioxide; cycloheximide; grassland soils; microbial biomass; mineralization; necromass; nitrogen; oxytetracycline; regrowth; soil carbon; soil ecology; soil respiration
Abstract:
... Biocides have been frequently used to understand the roles of fungi and bacteria on soil carbon (C) and nitrogen (N) cycling. However, addition of biocides to soil can result in unwanted temporary increases in C and N supply to surviving microbes due to a pulse in microbial necromass, and where biocides can directly be used as sources of C and N. We assessed temporary dynamics in microbial biomass ...
DOI:
10.1016/j.apsoil.2020.103862

37. Biodegradation of mycotoxin patulin by the yeast Meyerozyma guilliermondii

Author:
Yu Fu; Qiya Yang; Dhanasekaran Solairaj; Esa A. Godana; Michael N. Routledge; Hongyin Zhang
Source:
Biological control 2021 v.160 pp. 104692
ISSN:
1049-9644
Subject:
Meyerozyma guilliermondii; Penicillium expansum; adsorption; animal and human health; biodegradation; biological control; cell walls; cycloheximide; fungal antagonists; metabolites; molds (fungi); oxidoreductases; patulin; yeasts
Abstract:
... Patulin is a well-known mycotoxin widely found in rotten fruits, produced by a series of toxigenic fungi, particularly Penicillium expansum. Patulin is hazardous to human and animal health. Meyerozyma guilliermondii is an antagonistic yeast that has been found to have biocontrol properties against blue mold disease in pears. But, it is not clear whether M. guilliermondii can degrade patulin. The p ...
DOI:
10.1016/j.biocontrol.2021.104692

38. Cyclooxygenase-2 Glycosylation Is Affected by Peroxynitrite in Endothelial Cells: Impact on Enzyme Activity and Degradation

Author:
Sonia Eligini; Susanna Colli; Aida Habib; Giancarlo Aldini; Alessandra Altomare; Cristina Banfi
Source:
Antioxidants 2021 v.10 no.3 pp. -
ISSN:
2076-3921
Subject:
Western blotting; cycloheximide; enzyme activity; glycolysis; glycosylation; hexokinase; humans; nuclear membrane; precipitin tests; prostaglandin synthase; prostaglandins
Abstract:
... The exposure of human endothelial cells to 3-morpholinosydnonimine (SIN-1) induced the expression of cyclooxygenase-2 (COX-2) in a dose- and time-dependent manner. Interestingly, after a prolonged incubation (>8 h) several proteoforms were visualized by Western blot, corresponding to different states of glycosylation of the protein. This effect was specific for SIN-1 that generates peroxynitrite a ...
DOI:
10.3390/antiox10030496

39. Discovery of C13-Aminobenzoyl Cycloheximide Derivatives that Potently Inhibit Translation Elongation

Author:
Yumi Koga; Eileen M. Hoang; Yongho Park; Alexander F.A. Keszei; Jason Murray; Sichen Shao; Brian B. Liau
Source:
Journal of the American Chemical Society 2021 v.143 no.34 pp. 13473-13477
ISSN:
1520-5126
Subject:
cycloheximide; electron microscopy; polyribosomes; protein synthesis
Abstract:
... Employed for over half a century to study protein synthesis, cycloheximide (CHX, 1) is a small molecule natural product that reversibly inhibits translation elongation. More recently, CHX has been applied to ribosome profiling, a method for mapping ribosome positions on mRNA genome-wide. Despite CHX’s extensive use, CHX treatment often results in incomplete translation inhibition due to its rapid ...
DOI:
10.1021/jacs.1c05146

40. The E3 ubiquitin ligase SCF(Fbxo7) mediates proteasomal degradation of UXT isoform 2 (UXT-V2) to inhibit the NF-κB signaling pathway

Author:
Valentine Spagnol; Caio A.B. Oliveira; Suzanne J. Randle; Patrícia M.S. Passos; Camila R.S.T.B. Correia; Natália B. Simaroli; Joice S. Oliveira; Tycho E.T. Mevissen; Ana Carla Medeiros; Marcelo D. Gomes; David Komander; Heike Laman; Felipe Roberti Teixeira
Source:
Biochimica et biophysica acta 2021 v.1865 no.1 pp. 129754
ISSN:
0304-4165
Subject:
F-box proteins; alternative splicing; androgens; apoptosis; cycloheximide; estrogens; microscopy; precipitin tests; transcription (genetics); ubiquitin; ubiquitin-protein ligase; ubiquitination
Abstract:
... Ubiquitously eXpressed Transcript isoform 2 (UXTV2) is a prefoldin-like protein involved in NF-κB signaling, apoptosis, and the androgen and estrogen response. UXT-V2 is a cofactor in the NF-κB transcriptional enhanceosome, and its knockdown inhibits TNF-α -induced NF-κB activation. Fbxo7 is an F-box protein that interacts with SKP1, Cullin1 and RBX1 proteins to form an SCF(Fbxo7) E3 ubiquitin lig ...
DOI:
10.1016/j.bbagen.2020.129754

41. Effect of Rhodotorula mucilaginosa on patulin degradation and toxicity of degradation products

Author:
Qiya Yang; Bo Pang; Dhanasekaran Solairaj; Weicheng Hu; Ngolong Ngea Guillaume Legrand; Junfang Ma; Siyao Huang; Xiangyang Wu; Hongyin Zhang
Source:
Food additives & contaminants 2021 v.38 no.8 pp. 1427-1439
ISSN:
1944-0057
Subject:
Rhodotorula rubra; apoptosis; biological control; cell walls; cycloheximide; fungal antagonists; hepatoma; humans; metabolites; patulin; toxicity; toxicity testing
Abstract:
... Rhodotorula mucilaginosa is an antagonistic yeast for which our research team has recently reported interesting biocontrol activities against blue mould decay of apples and a strong ability to decrease the patulin concentration in vivo. However, the possible mechanisms of patulin degradation by R. mucilaginosa and the toxicity of patulin degradation products remain unclear. In this study, the effe ...
DOI:
10.1080/19440049.2021.1923821

42. Effect of lactic acid bacteria and yeasts on the structure and fermentation properties of Tibetan kefir grains

Author:
Zhina Chen; Tian Liu; Tao Ye; Xijuan Yang; Yongzhen Xue; Yeqiao Shen; Qing Zhang; Xiaomei Zheng
Source:
International dairy journal 2021 v.114 pp. 104943
ISSN:
0958-6946
Subject:
Lactobacillus; cycloheximide; fermentation; kefir; lactic acid; odors; yeasts
Abstract:
... To better understand the effects of lactic acid bacteria (LAB) and yeasts on the structure and fermentation properties of Tibetan kefir grains (TKGs), we treated TKGs for 1 month with high doses of mycillin and cycloheximide to inhibit LAB and yeasts, respectively. The results showed that in each group of TKGs, Lactobacillus kefiranofaciens was the only dominant and stable bacterial species, and i ...
DOI:
10.1016/j.idairyj.2020.104943

43. Effects of Incubation Time and Method of Cell Cycle Synchronization on Collared Peccary Skin-Derived Fibroblast Cell Lines

Author:
Alana Azevedo Borges; Maria Claudia dos Santos Luciano; Matheus Barbosa do Nascimento; Gabriela Pereira de Oliveira Lira; Fátima de Cássia Evangelista de Oliveira; Claudia Pessoa; Alexsandra Fernandes Pereira
Source:
Annals of animal science 2021 v.21 no.3 pp. 925-938
ISSN:
2300-8733
Subject:
Pecari tajacu; animal science; blood serum; cell cycle; cycloheximide; cytochalasin B; dimethyl sulfoxide; fibroblasts; flow cytometry; somatic cells; starvation; viability
Abstract:
... The success of cloning by somatic cell nuclear transfer depends on the efficiency of nuclear reprogramming, with the cycle stage of the donor cell playing a crucial role. Therefore, the aim was to evaluate three different approaches for cell cycle synchronization: (i) serum starvation (SS) for 1 to 4 days, (ii) contact inhibition (CI) for 1 to 3 days, and (iii) using cell cycle regulatory inhibito ...
DOI:
10.2478/aoas-2020-0103

44. Enhanced GRP78 protein expression via the IRE1α/ASK1/p38 MAPK pathway during As2O3-induced endoplasmic reticulum stress in BEAS-2B cells

Author:
Jiaming Yuan; Chenjuan Yao; Jing Tang; Yingqi Liu; Chunyan Huang; Shali Yu; Haiyan Wei; Yu Han; Gang Chen
Source:
Toxicology 2021 v.462 pp. 152962
ISSN:
0300-483X
Subject:
actinomycin D; air; apoptosis; arsenic; arsenic oxide; biosynthesis; cell viability; cycloheximide; endoplasmic reticulum; endoplasmic reticulum stress; gene expression; mitogen-activated protein kinase; models; phosphorylation; protein synthesis inhibitors; ribonucleases; toxicology; unfolded protein response
Abstract:
... Inorganic arsenic is widely present in the environment. Exposure to moderate to high concentrations of arsenic from drinking water or air can cause various cancers and multisystem dysfunction. Glucose-regulated protein 78 (GRP78) is an endoplasmic reticulum (ER) stress sensor of unfolded protein response (UPR) under stress conditions and it enhances cell survival. The aim of this study is to inves ...
DOI:
10.1016/j.tox.2021.152962

45. Estrogen activates endothelial exocytosis

Author:
Christine S. Kim; Kyungmoo Yea; Craig N. Morrell; Youngtae Jeong; Charles J. Lowenstein
Source:
Biochemical and biophysical research communications 2021 v.558 pp. 29-35
ISSN:
0006-291X
Subject:
adhesion; agonists; antagonists; blood coagulation factors; cycloheximide; embolism; exocytosis; inflammation; interleukin-8; mitogen-activated protein kinase; osteoporosis; postmenopause; research; risk; therapeutics; thrombosis
Abstract:
... Estrogen therapy is used to treat patients with post-menopausal symptoms, such as hot flashes and dyspareunia. Estrogen therapy also decreases the risk of fractures from osteoporosis in post-menopausal women. However, estrogen increases the risk of venous thromboembolic events, such as pulmonary embolism, but the pathways through which estrogen increase the risk of thromboembolism is unknown. Here ...
DOI:
10.1016/j.bbrc.2021.04.019

46. FBX4 mediates rapid cyclin D1 proteolysis upon DNA damage in immortalized esophageal epithelial cells

Author:
Jia Liu; Hui Yang; Pak Yan Cheung; Sai Wah Tsao; Liang Lv; Annie L.M Cheung
Source:
Biochemical and biophysical research communications 2021 v.554 pp. 76-82
ISSN:
0006-291X
Subject:
DNA; DNA damage; carcinogenesis; cell cycle checkpoints; cyclins; cycloheximide; epithelium; esophageal neoplasms; genetic instability; irradiation; proteolysis; research; squamous cell carcinoma; ubiquitin-protein ligase
Abstract:
... It has been implied that deregulation of cyclin D1 turnover under stresses can facilitate genomic instability and trigger tumorigenesis. Much focus has been placed on identifying the E3 ligases responsible for mediating cyclin D1 degradation. However, the findings were quite controversial and cell type-dependent. Little is known about how cyclin D1 is regulated in precancerous cells upon DNA damag ...
DOI:
10.1016/j.bbrc.2021.03.089

47. Factors that affect protein abundance of a positive regulator of abscisic acid signalling, the basic leucine zipper transcription factor ABRE‐binding factor 2 (ABF2)

Author:
Katrina J. Linden; Yi‐Tze Chen; Khin Kyaw; Brandan Schultz; Judy Callis
Source:
Plant direct 2021 v.5 no.6 pp. e00330
ISSN:
2475-4455
Subject:
Arabidopsis thaliana; abscisic acid; cycloheximide; germination; leucine zipper; proteasome inhibitors; protein binding; proteolysis; seed maturation; transcription factors; ubiquitin; ubiquitin-protein ligase; ubiquitination; vegetative growth
Abstract:
... Most members of basic leucine zipper (bZIP) transcription factor (TF) subgroup A play important roles as positive effectors in abscisic acid (ABA) signaling during germination and/or in vegetative stress responses. In multiple plant species, one member, ABA insensitive 5 (ABI5), is a major TF that promotes seed maturation and blocks early seeding growth in response to ABA. Other members, referred ...
DOI:
10.1002/pld3.330

48. Fenofibrate inhibits hypoxia‐inducible factor‐1 alpha and carbonic anhydrase expression through activation of AMP‐activated protein kinase/HO‐1/Sirt1 pathway in glioblastoma cells

Author:
Chingju Lin; Sheng‐Wei Lai; Ching‐Kai Shen; Chao‐Wei Chen; Cheng‐Fang Tsai; Yu‐Shu Liu; Dah‐Yuu Lu; Bor‐Ren Huang
Source:
Environmental toxicology 2021 v.36 no.12 pp. 2551-2561
ISSN:
1520-4081
Subject:
AMP-activated protein kinase; Food and Drug Administration; agonists; carbonate dehydratase; cycloheximide; death; ecotoxicology; fenofibrate; glioblastoma; humans; hyperlipidemia; hypoxia-inducible factor 1; monitoring; proteasome inhibitors; protein degradation; protein synthesis; protein synthesis inhibitors; public health
Abstract:
... Cancer and its associated conditions have significant impacts on public health at many levels worldwide, and cancer is the leading cause of death among adults. Peroxisome proliferator‐activated receptor α (PPARα)‐specific agonists, fibrates, have been approved by the Food and Drug Administration for managing hyperlipidemia. PPARα‐specific agonists exert anti‐cancer effects in many human cancer typ ...
DOI:
10.1002/tox.23369

49. Halofuginone triggers a transcriptional program centered on ribosome biogenesis and function in honey bees

Author:
Melissa E. Flores; Nora K. McNamara-Bordewick; Natalie L. Lovinger; Jonathan W. Snow
Source:
Insect biochemistry and molecular biology 2021 v.139 pp. 103667
ISSN:
0965-1748
Subject:
Nosema ceranae; amino acids; biogenesis; cycloheximide; gene expression; halofuginone; honey; insect biochemistry; molecular biology; pollinators; quality control; ribosomes; stress response; therapeutics; toxicity; transcription (genetics); unfolded protein response
Abstract:
... We previously found that pharmacological inhibition of prolyl-tRNA synthetase by halofuginone has potent activity against Nosema ceranae, an important pathogen of honey bees. However, we also observed that prolyl-tRNA synthetase inhibition is toxic to bees, suggesting further work is necessary to make this a feasible therapeutic strategy. As expected, we found that pharmacological inhibition of pr ...
DOI:
10.1016/j.ibmb.2021.103667

50. In vitro and in silico anticancer potential analysis of Streptomyces sp. extract against human lung cancer cell line, A549

Author:
Prateek Kumar; Anjali Chauhan; Munendra Kumar; Bijoy K. Kuanr; Aditi Kundu; Renu Solanki; Monisha Khanna Kapur
Source:
3 Biotech 2021 v.11 no.6 pp. 254
ISSN:
2190-572X
Subject:
Streptomyces; alkaloids; antineoplastic activity; apoptosis; cell viability; computer simulation; cycloheximide; cytoskeleton; cytotoxicity; energy; humans; lung neoplasms; metastasis; mutants; neoplasm cells; phalloidine; therapeutics
Abstract:
... During our previous investigation, bioactive compounds present in the extract of Streptomyces sp. strain 196 were characterized using LC–MS/MS and ¹H NMR studies. These compounds were K-252-C aglycone indolocarbazole alkaloid, decoyinine, and cycloheximide; the study of these natural drugs against lung carcinoma is still limited. Focus of the current investigation was to study the anticancer effec ...
DOI:
10.1007/s13205-021-02812-w