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You searched for: Subject derivatization Remove constraint Subject: derivatization Subject term chemical species Remove constraint Subject term: chemical species Subject term acetic acid Remove constraint Subject term: acetic acid Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. A rapid and robust method for amino acid quantification using a simple N-hydroxysuccinimide ester derivatization and liquid chromatography-ion mobility-mass spectrometry

Author:
Taylor M. Domenick; Austin L. Jones; Robin H. J. Kemperman; Richard A. Yost
Source:
Analytical and bioanalytical chemistry 2022 v.414 no.18 pp. 5549-5559
ISSN:
1618-2642
Subject:
acetic acid; amino acids; analytical chemistry; chemical species; derivatization; mass spectrometry; metabolites; metabolomics; neuroglia; reversed-phase liquid chromatography; yeast extract
Abstract:
... The vast majority of mass spectrometry (MS)-based metabolomics studies employ reversed-phase liquid chromatography (RPLC) to separate analytes prior to MS detection. Highly polar metabolites, such as amino acids (AAs), are poorly retained by RPLC, making quantitation of these key species challenging across the broad concentration ranges typically observed in biological specimens, such as cell extr ...
DOI:
10.1007/s00216-022-03993-w

2. Determination of S-adenosylmethionine and S-adenosylhomocysteine in blood plasma by UPLC with fluorescence detection

Author:
Alexander Vladimirovich Ivanov; Ekaterina Alexandrovna Dubchenko; Maria Petrovna Kruglova; Edward Danielevich Virus; Polina Olegovna Bulgakova; Valery Vasil'evich Alexandrin; Anatolij Nikolaevich Fedoseev; Alexey Nikolaevich Boyko; Sergej Vitalievich Grachev; Aslan Amirkhanovich Kubatiev
Source:
Journal of chromatography 2019 v.1124 pp. 366-374
ISSN:
1570-0232
Subject:
S-adenosylmethionine; acetic acid; acetonitrile; blood plasma; chemical species; derivatization; detection limit; fluorescence; metabolites; phenylboronic acids; potassium dihydrogen phosphate; solid phase extraction; ultra-performance liquid chromatography; volunteers
Abstract:
... A validated approach to determine various methionine cycle metabolites (S-adenosylmethionine, S-adenosylhomocysteine, and methylthioadenosine) in human blood plasma is offered. The approach is based on solid-phase extraction (with grafted phenylboronic acid) and derivatization with chloroacetaldehyde followed by ultra-performance liquid chromatography with fluorescence detection. We used a 100 × 2 ...
DOI:
10.1016/j.jchromb.2019.06.032

3. Development and validation of a GC-FID method for the analysis of short chain fatty acids in rat and human faeces and in fermentation fluids

Author:
Serena Scortichini; Maria Chiara Boarelli; Stefania Silvi; Dennis Fiorini
Source:
Journal of chromatography 2020 v.1143 pp. 121972
ISSN:
1570-0232
Subject:
acetic acid; acidification; chemical species; derivatization; ethyl ether; feces; fermentation; flame ionization; hexanoic acid; humans; intestinal microorganisms; metabolites; mice; polyethylene glycol; propionic acid; quantitative analysis; rats
Abstract:
... Short-chain fatty acids (SCFAs) are gut microbiota metabolites recognized for their beneficial effects on the host organism. In this study, a simple and rapid sample preparation method combined to SCFAs analysis by direct injection and gas chromatography coupled with flame ionization detection (GC-FID), for the determination and quantification of eight SCFAs (acetic, propionic, i-butyric, butyric, ...
DOI:
10.1016/j.jchromb.2020.121972