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You searched for: Subject disaccharides Remove constraint Subject: disaccharides Subject term medicine Remove constraint Subject term: medicine Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Efficient expression of chondroitinase ABC I for specific disaccharides detection of chondroitin sulfate

Author:
Xingyu Lu; Qian Zhong; Jian Liu; Fulin Yang; Chenghui Lu; Huan Xiong; Sha Li; Yibo Zhu; Lingtian Wu
Source:
International journal of biological macromolecules 2020 v.143 pp. 41-48
ISSN:
0141-8130
Subject:
Escherichia coli; chondroitin sulfate; culture flasks; disaccharides; enzymes; fermenters; inclusion bodies; medicine; response surface methodology; signal peptide
Abstract:
... Chondroitinase ABC I (ChSase ABC I) is a key enzyme of chondroitin sulfate (CS) degradation and widely used for CS detection in the medicine filed. However, the recombinant ChSase ABC I was weakly expressed in Escherichia coli because the forms of it were mostly inclusion bodies. In this study, a signal peptide (pelB) was used for the soluble form expression of ChSase ABC I in E. coli. Then the cu ...
DOI:
10.1016/j.ijbiomac.2019.11.215

2. Exploring dry storage as an alternative biobanking strategy inspired by Nature

Author:
Joseph Saragusty; Pasqualino Loi
Source:
Theriogenology 2019 v.126 pp. 17-27
ISSN:
0093-691X
Subject:
DNA; animal production; bioactive properties; bulls; cryopreservation; disaccharides; freeze drying; freezing; hamsters; heat shock proteins; horses; industry; laboratory animals; medicine; mice; nitrogen; progeny; rabbits; rats; rehydration; somatic cells; spermatozoa; wildlife management
Abstract:
... Biobanking is a rapidly growing industry, covering diverse fields such as human medicine, farm animal production, laboratory animals record keeping, and wildlife conservation. Presently, biobanking is done almost exclusively by cryopreservation, followed by maintenance of the samples under liquid nitrogen. Cryopreservation has satisfactory efficiency but it comes with a host of problems, and the p ...
DOI:
10.1016/j.theriogenology.2018.11.027

3. Neovascularization Induced by the Hyaluronic Acid-Based Spongy-Like Hydrogels Degradation Products

Author:
Silva Lucília P. da; Pirraco Rogério P.; Santos Tírcia C.; Novoa-Carballal Ramon; Cerqueira Mariana T.; Reis Rui L.; Correlo Vitor M.; Marques Alexandra P.
Source:
ACS Applied Materials & Interfaces 2016 v.8 no.49 pp. 33464-33474
ISSN:
1944-8252
Subject:
angiogenesis; biodegradation; disaccharides; endothelial cells; gellan gum; hindlimbs; humans; hyaluronic acid; hydrogels; medicine; receptors; tissue engineering; tissue repair; umbilical cord
Abstract:
... Neovascularization has been a major challenge in many tissue regeneration strategies. Hyaluronic acid (HA) of 3–25 disaccharides is known to be angiogenic due to its interaction with endothelial cell receptors. This effect has been explored with HA-based structures but a transitory response is observed due to HA burst biodegradation. Herein we developed gellan gum (GG)-HA spongy-like hydrogels fro ...
DOI:
10.1021/acsami.6b11684