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You searched for: Subject disease resistance Remove constraint Subject: disease resistance Subject DNA primers Remove constraint Subject: DNA primers Text Availability Full Text Remove constraint Text Availability: Full Text
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1. Identification of Mx gene nucleotide dimorphism (G/A) as genetic marker for antiviral activity in Egyptian chickens

Author:
Mohamed S. Hassanane; Amal A.M. Hassan; Fatma M. Ahmed; Esteftah M. El-Komy; Khaled M. Roushdy; Nagwa A. Hassan
Source:
Journal of Genetic Engineering and Biotechnology (Print) 2018 v.16 no.1 pp. 83-88
ISSN:
1687-157X
Subject:
DNA; DNA fragmentation; DNA primers; alleles; antiviral properties; biotechnology; breeding programs; chicken breeds; chickens; dimorphism; disease resistance; exons; gene frequency; genetic engineering; genetic markers; genotype; genotyping; livestock breeding; polymerase chain reaction; restriction endonucleases; restriction fragment length polymorphism; Egypt
Abstract:
... Egyptian chickens, representing 2 breeds and 7 strains, were genotyped using the PCR-RFLP and sequencing techniques for detection of a non-synonymous dimorphism (G/A) in exon 14 of chicken Myxovirus resistance (Mx) gene. This dimorphic position is responsible for altering Mx protein’s antiviral activity. Polymerase Chain reactions were performed using Egyptian chickens DNA and specific primer set ...
DOI:
10.1016/j.jgeb.2017.11.002
PubMed:
30647709
PubMed Central:
PMC6296577

2. Discovery and characterization of a molecular marker for Sclerotinia minor (Jagger) resistance in peanut

Author:
Chenault, Kelly D.; Maas, Andrea L.; Damicone, John P.; Payton, Mark E.; Melouk, Hassan A.
Source:
Euphytica 2009 v.166 no.3 pp. 357-365
ISSN:
0014-2336
Subject:
Arachis hypogaea; peanuts; disease resistance; Sclerotinia minor; blight; fungal diseases of plants; genetic markers; microsatellite repeats; polymerase chain reaction; DNA primers; genotype
Abstract:
... The production of cultivated peanut, an important agronomic crop throughout the United States and the world, is consistently threatened by various diseases and pests. Sclerotinia minor Jagger (S. minor), the causal agent of Sclerotinia blight, is a major threat to peanut production in the Southwestern US, Virginia and North Carolina. Although information on the variability of morphological traits ...
Handle:
10113/27152
DOI:
10.1007/s10681-008-9816-0

3. Resistance of wheat to Mycosphaerella graminicola involves early and late peaks of gene expression

Author:
Tika B. Adhikari; Boovaraghan Balaji; Jill Breeden
Source:
Physiological and molecular plant pathology 2007 v.71 pp. 55-68
ISSN:
0885-5765
Subject:
Mycosphaerella graminicola; DNA primers; signal transduction; wheat; genes; microbial growth; phenylalanine ammonia-lyase; peptidylprolyl isomerase; gene expression; ribosomal proteins; disease resistance; peroxidase; pathogens; adenosinetriphosphatase; cultivars; research programs; polymerase chain reaction; chitinase; resistance mechanisms; proteinase inhibitors; ribonucleases; gene induction; fungi
Abstract:
... Large-scale cDNA-AFLP profiling identified numerous genes with increased expression during the resistance response of wheat to the Septoria tritici blotch fungus, Mycosphaerella graminicola. To test whether these genes were associated with resistance responses, primers were designed for the 14 that were most strongly up-regulated, and their levels of expression were measured at 12 time points from ...
Handle:
10113/57436
DOI:
10.1016/j.pmpp.2007.10.004

4. Resistance gene analogs associated with Fusarium head blight resistance in wheat

Author:
Guo, Pei-Guo; Bai, Gui-Hua; Li, Rong-Hua; Shaner, Gregory; Baum, Michael
Source:
Euphytica 2006 v.151 no.2 pp. 251-261
Subject:
quantitative trait loci; Fusarium graminearum; DNA primers; linkage groups; polymerase chain reaction; wheat; chromosome mapping; Triticum aestivum; disease resistance; scab diseases; genes; genetic markers
Abstract:
... Fusarium head blight (FHB) is one of the most destructive diseases in wheat. Identification of resistance gene analogs (RGAs) may provide candidate genes for cloning of FHB resistance genes and molecular markers for marker-assisted improvement of wheat FHB resistance. To identify potential RGAs associated with FHB resistance in wheat, 18 primer pairs of RGAs were screened between two parents (Ning ...
Handle:
10113/28050
DOI:
10.1007/s10681-006-9153-0

5. Development of a SNLP marker from the Pi-ta blast resistance gene by tri-primer PCR

Author:
Jia, Y.; Redus, M.; Wang, Z.; Rutger, J.N.
Source:
Euphytica 2004 v.138 no.1 pp. 97-105
ISSN:
0014-2336
Subject:
cultivars; rice; blast disease; alleles; Oryza sativa; DNA primers; polymerase chain reaction; disease resistance; plant pathogenic fungi; Magnaporthe grisea; genetic markers
Abstract:
... The Pi-ta gene from indica introgressed into japonica rice has been used to control the blast disease caused by the fungal pathogen Magnaporthe grisea (Herbert) Barr. (anamorph Pyricularia oryzae Cav.) worldwide. A single nucleotide length polymorphism (SNLP) was identified at the intron region of the Pi-ta gene to develop a codominant Pi-ta gene marker suitable for genotyping with an automated ma ...
Handle:
10113/10674
DOI:
10.1023/B:EUPH.0000047079.42768.4d

6. In vivo effects of CpG oligodeoxynucleotide on Eimeria infection in chickens

Author:
Dalloul, R.A.; Lillehoj, H.S.; Okamura, M.; Xie, H.; Min, W.; Ding, X.; Heckert, R.A.
Source:
Avian diseases 2004 v.48 no.4 pp. 783-790
ISSN:
0005-2086
Subject:
chickens; oocysts; coccidiosis; food animals; DNA primers; body weight; antibodies; poultry diseases; liveweight gain; Eimeria acervulina; nucleotide sequences; disease resistance; immune response; subunit vaccines
Abstract:
... Poultry coccidiosis is the major parasitic disease of poultry and, until now, no recombinant vaccine has been developed. Short oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs (CpG ODNs) have been shown to be effective immunoprotective agents and vaccine adjuvants in mammalian systems. Their use in poultry to protect against intracellular parasites has not been reported to date. The ...
Handle:
10113/36857
DOI:
10.1637/7154-010704R
PubMed:
15666859

7. Identification of resistance gene analogs in cotton (Gossypium hirsutum L.)

Author:
Tan, H.; Callahan, F.E.; Zhang, X.D.; Karaca, M.; Saha, S.; Jenkins, J.N.; Creech, R.G.; Ma, D.P.
Source:
Euphytica 2003 v.134 no.1 pp. 1-7
ISSN:
0014-2336
Subject:
Gossypium hirsutum; cotton; structural genes; plant proteins; binding proteins; disease resistance; polymerase chain reaction; DNA primers; nucleotide sequences; amino acid sequences
Abstract:
... Sequence analyses of numerous plant disease resistance genes have revealed the presence of conserved motifs common to this class of genes, namely a nucleotide binding site (NBS) and leucine rich repeat region. In this study, thirty-three resistance gene analogs (RGAs) were cloned and sequenced from cotton (Gossypium hirsutum L.) following PCR with degenerate primers designed from the conserved NBS ...
Handle:
10113/2845

8. Multiplex real-time PCR for monitoring Heterobasidion annosum colonization in Norway spruce clones that differ in disease resistance

Author:
Hietala, A.M.; Eikenes, M.; Kvaalen, H.; Solheim, H.; Fossdal, C.G.
Source:
Applied and environmental microbiology 2003 v.69 no.8 pp. 4413-4420
ISSN:
0099-2240
Subject:
Picea abies; forest trees; Heterobasidion annosum; plant pathogenic fungi; fungal diseases of plants; tree diseases; microbial colonization; clones; disease resistance; conifer needles; tissue culture; polymerase chain reaction; tree trunk; DNA primers; laccase; genes; monitoring; ergosterol; measurement; field experimentation; disease diagnosis
Abstract:
... A multiplex real-time PCR assay was developed to monitor the dynamics of the Picea abies-Heterobasidion annosum pathosystem. Tissue cultures and 32-year-old trees with low or high resistance to this pathogen were used as the host material. Probes and primers were based on a laccase gene for the pathogen and a polyubiquitin gene for the host. The real-time PCR procedure was compared to an ergostero ...
DOI:
10.1128/AEM.69.8.4413-4420.2003
PubMed:
12902224
PubMed Central:
PMC169156

9. Physical and Genetic Mapping of Wheat Kinase Analogs and NBS-LRR Resistance Gene Analogs

Author:
Lili Maleki; Justin D. Faris; Robert L. Bowden; Bikram S. Gill; John P. Fellers
Source:
Crop science 2003 v.43 no.2 pp. 660-670
ISSN:
0011-183X
Subject:
restriction fragment length polymorphism; hard red winter wheat; sequence alignment; DNA primers; polymerase chain reaction; amino acid sequences; Triticum aestivum; disease resistance; genes; nucleotide sequences; physical chromosome mapping
Abstract:
... Conserved motifs within resistance genes have been utilized in polymerase chain reaction (PCR)-based strategies to isolate resistance gene analogs (RGAs) and resistance gene-like (RGLs) sequences from many plant species. RGAs have the potential to serve as closely linked markers for marker-assisted breeding or even as resistance gene candidates. The objectives of this study were to clone, sequence ...
Handle:
10113/11528
DOI:
10.2135/cropsci2003.6600

10. QTL mapping of partial resistance in winter wheat to Stagonospora nodorum blotch

Author:
Czembor, Pawel C.; Arseniuk, Edward; Czaplicki, Andrzej; Song, Qijiang; Cregan, Perry B.; Ueng, Peter P.
Source:
Genome = 2003 v.46 no.4 pp. 546-554
ISSN:
1480-3321
Subject:
quantitative trait loci; leaf blotch; microsatellite repeats; phenotype; Parastagonospora nodorum; DNA primers; disease severity; chromosome mapping; Triticum aestivum; disease resistance; doubled haploids; phenotypic variation; winter wheat; latent period
Abstract:
... Stagonospora nodorum blotch is an important foliar and glume disease in cereals. Inheritance of resistance in wheat appears to be quantitative. To date, breeding of partially resistant cultivars has been the only effective way to combat this pathogen. The partial resistance components, namely length of incubation period, disease severity, and length of latent period, were evaluated on a population ...
Handle:
10113/49371
DOI:
10.1139/g03-036

11. Quantifying Phytophthora medicaginis in susceptible and resistant alfalfa with a real-time fluorescent PCR assay

Author:
Vandemark, G.J.; Barker, B.M.
Source:
Phytopathologische Zeitschrift 2003 v.151 no.11-12 pp. 577-583
ISSN:
0931-1785
Subject:
cultivars; forage crops; quantitative analysis; DNA primers; polymerase chain reaction; Medicago sativa; root rot; alfalfa; resistance mechanisms; Phytophthora medicaginis; plant pathogenic fungi; disease resistance; plant response; genetic variation; nucleotide sequences; phenotypic variation; detection; fluorescent dyes
Abstract:
... A real-time fluorescent PCR assay using a set of specific primers and a fluorochrome-labelled probe (Taq-Man) was developed to quantify the amount of Phytophthora medicaginis DNA in alfalfa plants that were classified as either resistant or susceptible to the pathogen based on visual assessment of disease response. The assay clearly discriminated among three standard check alfalfa populations with ...
Handle:
10113/22804
DOI:
10.1046/j.0931-1785.2003.00768.x

12. Resistance gene homologues in Theobroma cacao as useful genetic markers

Author:
D. N. Kuhn; M. Heath; R. J. Wisser; A. Meerow; J. S. Brown; U. Lopes; R. J. Schnell
Source:
Theoretical and applied genetics 2003 v.107 no.2 pp. 191-202
ISSN:
0040-5752
Subject:
cultivars; microsatellite repeats; DNA primers; polymerase chain reaction; Theobroma cacao; amino acid sequences; chromosome mapping; genes; disease resistance; genetic markers
Abstract:
... Resistance gene homologue (RGH) sequences have been developed into useful genetic markers for marker-assisted selection (MAS) of disease resistant Theobroma cacao. A plasmid library of amplified fragments was created from seven different cultivars of cacao. Over 600 cloned recombinant amplicons were evaluated. From these, 74 unique RGHs were identified that could be placed into 11 categories based ...
Handle:
10113/12822
DOI:
10.1007/s00122-003-1239-7
PubMed:
12845435

13. A major QTL for common bacterial blight resistance derives from the common bean great northern landrace cultivar Montana No. 5

Author:
Miklas, P.N.; Coyne, D.P.; Grafton, K.F.; Mutlu, N.; Reiser, J.; Lindgren, D.T.; Singh, S.P.
Source:
Euphytica 2003 v.131 no.1 pp. 137-146
ISSN:
0014-2336
Subject:
plant pathogenic bacteria; cultivars; quantitative trait loci; beans; plant breeding; landraces; Xanthomonas axonopodis; DNA primers; polymerase chain reaction; genetic distance; Phaseolus vulgaris; crossing; genotype; disease resistance; Phaseolus acutifolius var. acutifolius; genes; leaves
Abstract:
... Knowledge of the evolutionary origin and sources of pest resistance genes will facilitate gene deployment and development of crop cultivars with durable resistance. Our objective was to determine the source of common bacterial blight (CBB) resistance in the common bean Great Northern Nebraska #1 (GN#1) and GN#1 Selection 27 (GN#1 Sel 27). Several great northern cultivars including GN#1, GN#1 Sel 2 ...
Handle:
10113/12425
DOI:
10.1023/A:1023064814531

14. PCR sampling of disease resistance-like sequences from a disease resistance gene cluster in soybean

Author:
Graham, M.A.; Marek, L.F.; Shoemaker, R.C.
Source:
Theoretical and applied genetics 2002 v.105 no.1 pp. 50-57
ISSN:
0040-5752
Subject:
Glycine max; DNA libraries; Phytophthora; complementary DNA; bacterial artificial chromosomes; polymerase chain reaction; DNA primers; powdery mildew; amino acid sequences; genes; disease resistance; nucleotide sequences; root rot
Abstract:
... Clusters of Resistance-like genes (RLGs) have been identified from a variety of plant species. In soybean, RLG-specific primers and BAC-fingerprinting were used to develop a contig of overlapping BACs for a cluster of RLGs on soybean linkage group J. The resistance genes Rps2 (Phytophthora stem and root rot) and Rmd-c (powdery mildew) and the ineffective nodulation gene Rj2 were previously mapped ...
Handle:
10113/12785