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1. (+)- and (−)-ganodilactone, a pair of meroterpenoid dimers with pancreatic lipase inhibitory activities from the macromycete Ganoderma leucocontextum

Author:
He-Ping Chen; Zhen-Zhu Zhao; Yu Zhang; Xue Bai; Ling Zhang; Ji-Kai Liu
Source:
RSC advances 2016 v.6 no.69 pp. 64469-64473
ISSN:
2046-2069
Subject:
Ganoderma; enzyme inhibition; fruiting bodies; inhibitory concentration 50; nuclear magnetic resonance spectroscopy; triacylglycerol lipase
Abstract:
... (+)- and (−)-Ganodilactone (1), a pair of novel meroterpenoid dimers possessing a unique 5′H-spiro[chroman-4,2′-furan]-2,5′-dione ring system, along with their biogeneticly-related compound, ganomycin I (2), were discovered from the fruiting bodies of Ganoderma leucocontextum. The structures and absolute configurations were assigned with the aid of 1D and 2D NMR spectra, HRESIMS analysis and ECD c ...
DOI:
10.1039/c6ra10638b

2. (+)-Myristinins A and D from Knema elegans, which inhibit DNA polymerase β and cleave DNA

Author:
Deng, J.Z.; Starck, S.R.; Li, S.; Hecht, S.M.
Source:
Journal of natural products 2005 v.68 no.11 pp. 1625-1628
ISSN:
0163-3864
Subject:
plant extracts; enzyme inhibitors; Myristicaceae; spectral analysis; flavonoids; DNA-directed DNA polymerase; enzyme inhibition; chemical structure; Thailand
DOI:
10.1021/np058064g

3. (3Z)-5-Chloro-3-(Hydroxyimino)indolin-2-one attenuates hyperglycemia, increased hepatic glycogen content and hepatic damage induced by malathion acute exposure in rats

Author:
Edina da Luz Abreu; Anne Suély Pinto Savall; Allyson Ardais Boneberg; Bianca Barreto Martins; Vanessa Carratú Gervini; Tuane Bazanella Sampaio; André Ricardo Fajardo; Natália Paroul; Daniel Henrique Roos; Simone Pinton
Source:
Nutrition & metabolism 2019 v.16 no.1 pp. 61
ISSN:
1743-7075
Subject:
acute exposure; adults; aspartate transaminase; blood glucose; catalase; cholinesterase; enzyme inhibition; gardens; glucose; glutathione-disulfide reductase; glycogen; homeostasis; hyperglycemia; laboratory animals; liver; liver function; malathion; males; oxidative stress; rats
Abstract:
... BACKGROUND: Organophosphorus pesticides (OP’s) are heavily constituted in agriculture, gardens, home and veterinary and although it is useful, there are concerns about the environment, safety and health of human and animals. In this study, we investigated the effects of a new oxime, (3Z)-5-Chloro-3-(Hydroxyimino)indolin-2-one (OXIME) against the alterations induced by malathion, an OP insecticide, ...
DOI:
10.1186/s12986-019-0374-3
PubMed:
31516541
PubMed Central:
PMC6727524

4. (‒)-Pteroside N and pterosinone, new BACE1 and cholinesterase inhibitors from Pteridium aquilinum

Author:
Yun-Hyeok Choi; Chun Whan Choi; Jin Kyu Kim; Wonsik Jeong; Gil Hong Park; Seong Su Hong
Source:
Phytochemistry letters 2018 v.27 pp. 63-68
ISSN:
1874-3900
Subject:
Pteridium aquilinum; acetylcholinesterase; amyloid; cholinesterase; cholinesterase inhibitors; enzyme inhibition; fractionation; glycosides; inhibitory concentration 50; nuclear magnetic resonance spectroscopy; sesquiterpenoids; spectral analysis
Abstract:
... Bioassay-guided fractionation of the ethanolic extract from the whole plants of Pteridium aquilinum has resulted in the isolation of a new pterosin glycoside, (‒)-pteroside N (1), and a new seco-illudoid sesquiterpene, pterosinone (2). Their structures were identified by analysis of the spectroscopic data including extensive 2D NMR. All of the isolates were evaluated for the anti-Alzheimer disease ...
DOI:
10.1016/j.phytol.2018.06.021

5. 1,8-Dihydroxynaphthalene (DHN)-melanin biosynthesis inhibitors increase erythritol production in Torula corallina, and DHN-melanin inhibits erythrose reductase

Author:
Lee, J.K.; Jung, H.M.; Kim, S.Y.
Source:
Applied and environmental microbiology 2003 v.69 no.6 pp. 3427-3434
ISSN:
0099-2240
Subject:
Torula; yeasts; erythritol; biosynthesis; sweet-tasting compounds; food biotechnology; alcohol oxidoreductases; enzyme activity; enzyme inhibition; enzyme inhibitors; aromatic hydrocarbons; melanin; melanization; fermentation
Abstract:
... The yeast Torula corallina is a strong erythritol producer that is used in the industrial production of erythritol. However, melanin accumulation during culture represents a serious problem for the purification of erythritol from the fermentation broth. Melanin biosynthesis inhibitors such as 3,4-dihydroxyphenylalanine and 1,8-dihydroxynaphthalene (DHN)-melanin inhibitors were added to the T. cora ...
DOI:
10.1128/AEM.69.6.3427-3434.2003
PubMed:
12788746
PubMed Central:
PMC161539

6. 1-Deoxynojirimycin Inhibits Metastasis of B16F10 Melanoma Cells by Attenuating the Activity and Expression of Matrix Metalloproteinases-2 and -9 and Altering Cell Surface Glycosylation

Author:
Wang, Ran-Juh; Yang, Chun-Hung; Hu, Miao-Lin
Source:
Journal of agricultural and food chemistry 2010 v.58 no.16 pp. 8988-8993
ISSN:
0021-8561
Subject:
messenger RNA; cell movement; melanoma; metalloproteinases; glycosylation; mulberries; flow cytometry; mannose; metastasis; enzyme inhibitors; cell membranes; gene expression; binding sites; cell lines; enzyme inhibition; cell adhesion; anticarcinogenic activity
Abstract:
... 1-Deoxynojirimycin (1-DNJ), an iminosugar rich in mulberry, has been shown to possess antimetastatic potential. The antimetastatic mechanisms of 1-DNJ in melanoma B16F10 cells were studied, as were the antimetastatic activity (cell adhesion, migration, and invasion) of 1-DNJ, matrix metalloproteinases (MMP-2 and MMP-9), tissue inhibitors of metalloproteinase (TIMP-1 and TIMP-2) mRNA, and flow cyto ...
DOI:
10.1021/jf101401b
PubMed:
23654233

7. 1-MCP regulates ethylene biosynthesis and fruit softening during ripening of 'Tegan Blue' plum

Author:
Khan, A.S.; Singh, Z.
Source:
Postharvest biology and technology 2007 v.43 no.3 pp. 298-306
ISSN:
0925-5214
Subject:
ethylene production; biosynthesis; fruit quality; firmness; plums; postharvest treatment; enzyme activity; ripening; enzyme inhibition
Abstract:
... To investigate the effects of postharvest application of 1-MCP on ethylene production and fruit softening, activities of ethylene biosynthesis and fruit softening enzymes were measured during postharvest ripening of plum (Prunus salicina Lindl. cv. Tegan Blue) fruit after being exposed to 1-MCP (0, 0.5, 1.0 or 2.0 microliter L⁻¹) at 20 ± 1 °C for 24 h. Following the treatments, fruit were allowed ...
DOI:
10.1016/j.postharvbio.2006.10.005

8. 10-Hydroxy-2-decenoic acid from Royal jelly: A potential medicine for RA

Author:
Yang, Xin-Yu; Yang, De-sheng; Wei-Zhang; Wang, Jian-Min; Li, Chun-Yang; Hui-Ye; Lei, Kang-Fu; Chen, Xiu-Fang; Shen, Nian-Han; Jin, Li-Qin; Wang, Jian-Guang
Source:
Journal of ethnopharmacology 2010 v.128 no.2 pp. 314-321
ISSN:
0378-8741
Subject:
traditional medicine; transcription factors; messenger RNA; reversed-phase liquid chromatography; protein kinases; metalloproteinases; hydroxy fatty acids; signal transduction; biochemical pathways; enzyme inhibitors; tumor necrosis factor-alpha; royal jelly; cultured cells; enzyme-linked immunosorbent assay; DNA-binding proteins; fibroblasts; medicinal properties; enzyme inhibition; synovial fluid; rheumatoid arthritis
Abstract:
... Aim of the study: Rheumatoid arthritis synovial fibroblasts (RASFs) are known to produce matrix metalloproteinases (MMPs) and cause joint destruction. The purpose of this study is to develop a potential medicine for rheumatoid arthritis (RA). Materials and methods: To this end, first, the MMPs inhibition factor was purified from an alkali-solubilized fraction of RJ (Apis mellifera) by C18 reverse- ...
DOI:
10.1016/j.jep.2010.01.055
PubMed:
20138211

9. 14-3-3 protein and ubiquitin C acting as SjIAP interaction partners facilitate tegumental integrity in Schistosoma japonicum

Author:
Juntao Liu; Bikash Ranjan Giri; Yongjun Chen; Guofeng Cheng
Source:
International journal for parasitology 2019 v.49 no.5 pp. 355-364
ISSN:
0020-7519
Subject:
Schistosoma japonicum; adults; apoptosis; caspases; death; definitive hosts; enzyme activity; enzyme inhibition; immune response; immunohistochemistry; messenger RNA; parasites; public health; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; schistosomiasis; two hybrid system techniques; ubiquitin
Abstract:
... Schistosomiasis, caused by trematodes of the genus Schistosoma, remains an important public health issue. Adult schistosomes can survive in the definitive host for several decades, although they are subject to the host immune response. Consequently, understanding the mechanism underlying worm survival in the definitive hosts could aid in developing novel strategies against schistosomiasis. We prev ...
DOI:
10.1016/j.ijpara.2018.11.011

10. The 15-lipoxygenase inhibitory, antioxidant, antimycobacterial activity and cytotoxicity of fourteen ethnomedicinally used African spices and culinary herbs

Author:
Jean Paul Dzoyem; Victor Kuete; Lyndy J. McGaw; Jacobus N. Eloff
Source:
Journal of ethnopharmacology 2014 v.156 pp. 1-8
ISSN:
0378-8741
Subject:
2,2-diphenyl-1-picrylhydrazyl; Adansonia digitata; Aframomum; Mycobacterium aurum; Mycobacterium fortuitum; Mycobacterium smegmatis; Tamarindus indica; antibacterial properties; antioxidant activity; antioxidants; bromides; cytotoxicity; enzyme inhibition; flavonoids; fruits; herbs; infectious diseases; inflammation; inhibitory concentration 50; kidneys; lethal concentration 50; medicinal properties; minimum inhibitory concentration; monkeys; pain; spices; tetrazolium; traditional medicine; Africa
Abstract:
... Culinary herbs and spices are widely used ethnomedically across Africa. They are traditionally employed in the treatment of several ailments including inflammation disorders, pain alleviation and infectious diseases. Pharmacological studies are necessary to provide a scientific basis to substantiate their traditional use and safety. In this study, the 15-lipoxygenase inhibitory, antioxidant, antim ...
DOI:
10.1016/j.jep.2014.08.007

11. 15–Lipoxygenase inhibition of Commelina benghalensis, Tradescantia fluminensis, Tradescantia zebrina

Author:
Cean Socorro M. Alaba; Christine L. Chichioco-Hernandez
Source:
Asian Pacific Journal of Tropical Biomedicine 2014 v.4 pp. 184-188
ISSN:
2221-1691
Subject:
Commelina benghalensis; Tradescantia fluminensis; Tradescantia zebrina; absorbance; alkaloids; arachidonate 15-lipoxygenase; enzyme inhibition; enzyme inhibitors; ethyl acetate; flavonoids; hexane; inhibitory concentration 50; methanol; saponins; steroids; tannins; terpenoids
Abstract:
... To evaluate the 15-lipoxygenase inhibitory activity of the methanol leaf extracts of Commelina benghalensis, Tradescantia fluminensis (T. fluminensis) and Tradescantia zebrina.The inhibitory activity was evaluated using a spectrophotometric assay by observing the increase in absorbance at 234 nm due to the formation of the product 13-hydroperoxyoctadecadienoic acid. The extracts were also tested f ...
DOI:
10.1016/S2221-1691(14)60229-X
PubMed:
25182435
PubMed Central:
PMC3868787

12. 16,17-Dihydro gibberellin A5 competitively inhibits a recombinant Arabidopsis GA 3β-hydroxylase encoded by the GA4 gene

Author:
Zhou, R.; Yu, M.; Pharis, R.P.
Source:
Plant physiology 2004 v.135 no.2 pp. 1000-1007
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Escherichia coli; gibberellic acid; enzyme inhibition; mixed function oxidase; nucleotide sequences; gene expression regulation; plant proteins
Abstract:
... Ring D-modified gibberellin (GA) A5 and A20 derivatives are structurally similar to GA20 and GA9 (the precursors to growth-active GA1 and GA4) and, when applied to higher plants, especially grasses, can reduce shoot growth with concomitant reductions in levels of growth-active GAs and increases in levels of their immediate 3-deoxy precursors. The recombinant Arabidopsis GA 3-hydroxylase (AtGA3ox1) ...
DOI:
10.1104/pp.104.041509
PubMed:
15181202
PubMed Central:
PMC514134

13. 17-DMAG disrupted the autophagy flux leading to the apoptosis of acute lymphoblastic leukemia cells by inducing heat shock cognate protein 70

Author:
Gang Xu; Xiujuan Ma; Fang Chen; Di Wu; Jianing Miao; Yang Fan
Source:
Life sciences 2020 v.249 pp. 117532
ISSN:
0024-3205
Subject:
RNA interference; apoptosis; autophagosomes; autophagy; cathepsin D; cell lines; cell viability; children; children at risk; enzyme inhibition; flow cytometry; fluorescent antibody technique; gene expression; gene targeting; heat stress; heat-shock protein 90; image analysis; immunoblotting; lymphocytic leukemia; lysosomes; messenger RNA; neoplasm cells; patients; pharmacology; protein synthesis; staining; water solubility
Abstract:
... B-lineage acute lymphoblastic leukemia (B-ALL) is most common in children. We had reported heat shock protein 90 (Hsp90) over-expressed in high risk B-ALL children. 17-DMAG is a water soluble Hsp90 inhibitor, which was proved to be effective for advanced solid tumors and hematological malignancy. However, there is little research on its application in newly diagnosed B-ALL. And the detailed mechan ...
DOI:
10.1016/j.lfs.2020.117532

14. 20(S)-Protopanaxadiol blocks cell cycle progression by targeting epidermal growth factor receptor

Author:
Tiehua Zhang; Yuan Liang; Peng Zuo; Siyuan Jing; Tiezhu Li; Yongjun Wang; Chengyu Lv; Da Li; Jie Zhang; Zhengyi Wei
Source:
Food and chemical toxicology 2020 v.135 pp. 111017
ISSN:
0278-6915
Subject:
apoptosis; cell cycle checkpoints; computer simulation; enzyme inhibition; enzyme inhibitors; epidermal growth factor receptors; gene expression; gene expression regulation; genes; ginsenosides; interphase; lung neoplasms; metabolites; mitogen-activated protein kinase; molecular dynamics; neoplasm cells; phosphorylation; signal transduction; tissue repair; tyrosine
Abstract:
... 20(S)-Protopanaxadiol [20(S)-PPD], one of the metabolites of ginsenosides, was investigated to determine its potential mechanism for targeting to epidermal growth factor receptor (EGFR) pathway in lung cancer cell A549. Results of kinase inhibitory assay showed that 20(S)-PPD was an EGFR tyrosine kinase inhibitor. By binding to EGFR, 20(S)-PPD disrupted the EGFR/MAPK signaling. The expression of g ...
DOI:
10.1016/j.fct.2019.111017

15. A 20-kDa Protein with the GTP-Binding and Trypsin Inhibitory Activities from Glycine max

Author:
Toshifumi HIRATA; Shunsuke IZUMI; Seiichiro TSUJI
Source:
Bioscience, biotechnology, and biochemistry 1999 v.63 no.10 pp. 1816-1818
ISSN:
1347-6947
Subject:
Glycine max; Kunitz-type proteinase inhibitor; amino acid sequences; amino acids; cultured cells; enzyme inhibition; guanosine triphosphate; soybeans; trypsin
Abstract:
... A 20-kDa protein (p20) with a GTP binding activity was purified from the cultured cells of Glycine max (soybean). The amino acid sequence of p20 showed 65% identity in a 23 amino acid overlap against the Kunitz-type trypsin inhibitor of soybean reported. Furthermore, it was found that a Kunitz-type soybean trypsin inhibitor of commercial origin also binds GTP. ...
DOI:
10.1271/bbb.63.1816

16. 27 Effects of the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone on frozen–thawed bovine sperm

Author:
C. De Canditiis; N. Pagano; V. Longobardi; C. Zuchegna; M. A. Kosior; R. Annunziata; E. Parente; B. Gasparrini
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 139
ISSN:
1031-3613
Subject:
DNA fragmentation; analysis of variance; apoptosis; automation; bulls; caspases; cold tolerance; cryopreservation; embryo (animal); enzyme inhibition; enzyme inhibitors; flow cytometry; freeze-thaw cycles; in vitro fertilization; membrane potential; mitochondria; mitochondrial membrane; phase-contrast microscopy; planning; sperm motility; spermatozoa; straw; thawing; thermoregulation; viability; vitrification
Abstract:
... Semen cryopreservation is critical for appropriate planning of both AI and IVF trials, improving the benefit:cost ratio. However, cryopreservation induces damage in mammalian spermatozoa, resulting in decreased fertility (Medeiros et al. 2002 Theriogenology 57, 327-344). It is known that cryopreservation and thawing induce apoptosis in a variety of cells, including bovine sperm (Anzar et al. 2002 ...
DOI:
10.1071/RDv31n1Ab27

17. 29 kDa Trypsin from the Pyloric Ceca of Atlantic Bonito (Sarda sarda): Recovery and Characterization

Author:
Klomklao, S.; Benjakul, S.; Visessanguan, W.; Kishimura, H.; Simpson, B.K.
Source:
Journal of agricultural and food chemistry 2007 v.55 no.11 pp. 4548-4553
ISSN:
0021-8561
Subject:
chemical composition; trypsin; heat stability; Sarda sarda; gastric mucosa; purity; cecum; amino acid sequences; pH; fish; terminal repeat sequences; temperature; molecular weight; enzyme inhibition
Abstract:
... Trypsin from the pyloric ceca of Atlantic bonito (Sarda sarda) was purified and characterized with respect to its purity; molecular weight; sensitivity to temperature, pH, and inhibition; and N-terminal sequence. The purified trypsin had a molecular weight of 29 kDa as per sodium dodecyl sulfate polyacrylamide gel electrophoresis, and optimal activity was observed at pH 9 and 65 °C with BAPNA as a ...
DOI:
10.1021/jf063319x

18. 2D-SAR, Topomer CoMFA and molecular docking studies on avian influenza neuraminidase inhibitors

Author:
Bing Niu; Yi Lu; Jianying Wang; Yan Hu; Jiahui Chen; Qin Chen; Guangwu He; Linfeng Zheng
Source:
Computational and Structural Biotechnology Journal 2019 v.17 pp. 39-48
ISSN:
2001-0370
Subject:
avian influenza; binding sites; biotechnology; computational methodology; computer simulation; cutting; enzyme inhibition; enzyme inhibitors; human health; humans; models; poultry; sialidase; viruses
Abstract:
... Avian influenza is a serious zoonotic infectious disease with huge negative impacts on local poultry farming, human health and social stability. Therefore, the design of new compounds against avian influenza has been the focus in this field. In this study, computational methods were applied to investigate the compounds with neuraminidase inhibitory activity. First, 2D-SAR model was built to recogn ...
DOI:
10.1016/j.csbj.2018.11.007
PubMed:
30595814
PubMed Central:
PMC6305694

19. 2‐Methoxylated FA Display Unusual Antibacterial Activity Towards Clinical Isolates of Methicillin‐Resistant Staphylococcus aureus (CIMRSA) and Escherichia coli

Author:
Néstor M. Carballeira; Nashbly Montano; Christian Morales; Joseph Mooney; Xiomara Torres; Dakeishla Díaz; David J. Sanabria‐Rios
Source:
Lipids 2017 v.52 no.6 pp. 535-548
ISSN:
0024-4201
Subject:
DNA topoisomerase (ATP-hydrolysing); Escherichia coli; acids; antibacterial properties; antibiotic resistance; enzyme inhibition; inhibitory concentration 50; methicillin; methicillin-resistant Staphylococcus aureus; micelles
Abstract:
... The naturally occurring (6Z)‐(±)‐2‐methoxy‐6‐hexadecenoic acid (1) and (6Z)‐(±)‐2‐methoxy‐6‐octadecenoic acid (2) were synthesized in 7–8 steps with 38 and 13% overall yields, respectively, by using an acetylide coupling approach, which made it possible to obtain a 100% cis‐stereochemistry for the double bonds. In a similar fashion, the acetylenic analogs (±)‐2‐methoxy‐6‐hexadecynoic acid (3) and ...
DOI:
10.1007/s11745-017-4262-1
PubMed:
28523480
PubMed Central:
PMC5513723

20. The 2′,4′,6′-trihydroxyacetophenone isolated from Myrcia multiflora has antiobesity and mixed hypolipidemic effects with the reduction of lipid intestinal absorption

Author:
Ferreira, Eduardo Antonio; Gris, Eliana Fortes; Rebello, Jussara Matos; Correia, Joao Francisco Gomes; Oliveira, Luis Flavio Souza de; Wilhelm Filho, Danilo; Pedrosa, Rozangela Curi
Source:
Planta medica 2011 v.77 no.14 pp. 1569-1574
ISSN:
0032-0943
Subject:
blood serum; cholesterol; dietary fat; enzyme activity; enzyme inhibition; high fat diet; intestinal absorption; intestines; lovastatin; mice; olive oil; triacylglycerol lipase; triacylglycerols; weight gain
Abstract:
... This study evaluated the hypolipidemic and antiobesity effects of phloroacetophenone (2′,4′,6′-trihydroxyacetophenone, THA) isolated from Myrcia multiflora and their relationship with triglyceride (TG) intestinal absorption and pancreatic lipase activity inhibition. The hypolipidemic effect of THA was evaluated by acute (Triton WR-1339 treatment) and chronic assay (high-fat diet treatment), the an ...
DOI:
10.1055/s-0030-1270956
PubMed:
21472649

21. 3,4,5,6-Tetrahydroxyxanthone preserves intercellular communication by reduction of the endogenous nitric oxide synthase inhibitor level

Author:
Su-Jie Jia; Bi-Kui Zhang; Yong-Quan Lai; Han-Wu Deng; Yuan-Jian Li
Source:
Journal of Asian natural products research 2011 v.13 no.1 pp. 20-26
ISSN:
1477-2213
Subject:
arginine; atherosclerosis; cell communication; cell viability; connexins; cultured cells; endothelial cells; enzyme inhibition; gene expression regulation; lysophosphatidylcholine; medicinal properties; messenger RNA; nitric oxide synthase; protective effect; protein synthesis; reactive oxygen species; xanthones
Abstract:
... To observe the direct effects of 3,4,5,6-tetrahydroxyxanthone on connexin43 (Cx43) expression in cultured endothelial cells, cells were treated with lysophosphatidylcholine (LPC, 10 mg/l) for 24 h in the presence or absence of different concentrations of 3,4,5,6-tetrahydroxyxanthone (1, 3, or 10 μmol l⁻ ¹). The reactive oxygen species (ROS) production, cell viability, asymmetric dimethylarginine ( ...
DOI:
10.1080/10286020.2010.539181

22. 3-Nitroacridine derivatives arrest cell cycle at G0/G1 phase and induce apoptosis in human breast cancer cells may act as DNA-target anticancer agents

Author:
Qian Zhou; Chaoqun You; Cong Zheng; Yawen Gu; Hongchao Gu; Rui Zhang; Hongshuai Wu; Baiwang Sun
Source:
Life sciences 2018 v.206 pp. 1-9
ISSN:
0024-3205
Subject:
DNA; DNA damage; adverse effects; antineoplastic activity; antineoplastic agents; apoptosis; bacteriophages; binding capacity; bioavailability; breast neoplasms; cell cycle checkpoints; cell growth; cell proliferation; circular dichroism spectroscopy; dose response; enzyme inhibition; fluorescence microscopes; growth retardation; humans; interphase; mechanism of action; neoplasm cells; therapeutics; ultraviolet-visible spectroscopy
Abstract:
... DNA is considered to be one of the most promising targets for anticancer agents. Acridine analogues have anticancer activity based on DNA binding and topoisomerases inhibition. However, due to the side effects, resistance and low bioavailability, a few have entered into clinical usage and the mechanisms of action are not fully understood. Novel acridine derivatives are needed for effective cancer ...
DOI:
10.1016/j.lfs.2018.05.010

23. 3-O-methylquercetin more selectively inhibits phosphodiesterase subtype 3

Author:
Ko, W.C.; Chen, M.C.; Wang, S.H.; Lai, Y.H.; Chen, J.H.; Lin, C.N.
Source:
Planta medica 2003 v.69 no.4 pp. 310-315
ISSN:
0032-0943
Subject:
animal proteins; cyclic AMP; plant extracts; medicinal plants; spectral analysis; lungs; heart; phosphoric diester hydrolases; stems; enzyme inhibition; enzyme inhibitors; hydrolysis; enzyme activity; isozymes; guinea pigs; quercetin; fractionation; chemical constituents of plants; bark; Rhamnus; Taiwan
DOI:
10.1055/s-2003-38874
PubMed:
12709896

24. 3-hydroxycoumarin loaded vesicles for recombinant human tyrosinase inhibition in topical applications

Author:
Michele Schlich; Marco Fornasier; Mariella Nieddu; Chiara Sinico; Sergio Murgia; Antonio Rescigno
Source:
Colloids and surfaces 2018 v.171 pp. 675-681
ISSN:
0927-7765
Subject:
absorption; biosynthesis; chlorides; colloids; cytotoxicity; drugs; engineering; enzyme activity; enzyme inhibition; gels; humans; liquids; melanin; melanoma; melanosis; monophenol monooxygenase
Abstract:
... Tyrosinase is one of the key enzymes in mammalian melanin biosynthesis. Decreasing tyrosinase activity has been targeted for the prevention of conditions related to the hyperpigmentation of the skin, such as melasma and age spots. This paper is devoted to the engineering of vesicle formulations loaded with 3-hydroxycoumarin for topical pharmaceutical applications. At first, it was demonstrated the ...
DOI:
10.1016/j.colsurfb.2018.08.008

25. The 3-methyl-4-nitrophenol (PNMC) compromises airway epithelial barrier function

Author:
Yun-Fang An; Xiao-Rui Geng; Li-Hua Mo; Jiang-Qi Liu; Li-Teng Yang; Xiao-Wen Zhang; Zhi-Gang Liu; Chang-Qing Zhao; Ping-Chang Yang
Source:
Toxicology 2018 v.395 pp. 9-14
ISSN:
0300-483X
Subject:
air pollution; apoptosis; caspases; cell lines; enzyme inhibition; epithelial cells; mice; models; mucosa; nitrophenols; nose; pathogenesis; permeability
Abstract:
... It is recognized that the air pollution is associated with the pathogenesis of airway diseases. This study aims to elucidate the role of the 3-methyl-4-nitrophenol (PNMC), one of the components of diesel-exhaust particles, in compromising the airway epithelial barrier integrity.A549 cells, an airway epithelial cell line, were cultured to monolayers to be used as an in vitro epithelial barrier mode ...
DOI:
10.1016/j.tox.2018.01.001

26. 3D-quantitative structure–activity relationship and antiviral effects of curcumin derivatives as potent inhibitors of influenza H1N1 neuraminidase

Author:
Yanni Lai; Yiwen Yan; Shanghui Liao; Yun Li; Yi Ye; Ni Liu; Fang Zhao; Peiping Xu
Source:
Archives of pharmacal research 2020 v.43 no.5 pp. 489-502
ISSN:
0253-6269
Subject:
Influenza A virus; animal models; antiviral properties; computer simulation; curcumin; enzyme inhibition; epithelium; fluorescent antibody technique; human influenza; immune response; interferon-gamma; interleukin-6; lungs; mice; neoplasms; nucleoproteins; pathogenicity; quantitative structure-activity relationships; secretion; sialidase; therapeutics; viral pneumonia; virion
Abstract:
... Curcumin derivatives have been shown to inhibit replication of human influenza A viruses (IAVs). However, it is not clear whether curcumin and its derivatives can inhibit neuraminidase (NA) of influenza virus. In this study, a meaningful 3D quantitative structure–activity relationship model (comparative molecular field analysis R² = 0.997, q² = 0.527, s = 0.064, F = 282.663) was built to understan ...
DOI:
10.1007/s12272-020-01230-5

27. 3EZ,20Ac-ingenol, a catalytic inhibitor of topoisomerases, downregulates p-Akt and induces DSBs and apoptosis of DT40 cells

Author:
Yasuaki Fukuda; Masahiro Kanbe; Manami Watanabe; Katsuaki Dan; Keiichi Matsuzaki; Susumu Kitanaka; Shohei Miyata
Source:
Archives of pharmacal research 2013 v.36 no.8 pp. 1029-1038
ISSN:
0253-6269
Subject:
DNA; DNA damage; DNA topoisomerase; Euphorbia kansui; Western blotting; apoptosis; caspase-3; cell cycle; cell proliferation; enzyme activity; enzyme inhibition; gene expression regulation; medicinal plants
Abstract:
... We have previously reported that many ingenol compounds derived from Euphorbia kansui exhibit topoisomerase (topo) II inhibitory activity. Of these compounds, 3EZ,20Ac-ingenol inhibited topo I activity. Camptothecin, which inhibits the religation activity of topo I without interfering with the binding of topo I to DNA and induces topo I-mediated DNA cleavage, was used as a positive control. In thi ...
DOI:
10.1007/s12272-013-0108-4
PubMed:
23595550
PubMed Central:
PMC3731510

28. 4,5-Dihydropyrazole derivatives containing oxygen-bearing heterocycles as potential telomerase inhibitors with anticancer activity

Author:
Yin Luo; Yang Zhou; Jie Fu; Hai-Liang Zhu
Source:
RSC advances 2014 v.4 no.45 pp. 23904-23913
ISSN:
2046-2069
Subject:
Western blotting; active sites; antineoplastic activity; apoptosis; autophagy; enzyme inhibition; enzyme inhibitors; flow cytometry; humans; inhibitory concentration 50; models; moieties; neoplasm cells; oxygen; pyrazoles; stomach neoplasms; telomerase; telomeres
Abstract:
... Telomere and telomerase were closely related to the occurrence and development of some cancers. After the key active site of telomerase was identified, to enhance the ability of dihydropyrazole derivatives to inhibit telomerase, we designed a series of novel 4,5-dihydropyrazole derivatives containing heterocyclic oxygen moiety based on previous studies. The telomerase inhibition assay showed that ...
DOI:
10.1039/c4ra02200a

29. 47. In vitro assessment of apoptosis and necrosis following cold storage in Human Corneal Endothelial Cells

Author:
William L. Corwin; John M. Baust; Robert G. Van Buskirk; John G. Baust
Source:
Cryobiology 2013 v.66 no.3 pp. 355
ISSN:
0011-2240
Subject:
Western blotting; apoptosis; caspases; cell viability; cold; cold storage; cold stress; cold tolerance; cornea; depth; endothelial cells; enzyme inhibition; enzyme inhibitors; flow cytometry; fluorescence microscopy; fluorescent dyes; humans; in vitro studies; mitochondria; necrosis; population; proteins
Abstract:
... Corneal endothelial cells serve as a limiting factor in the ability to preserve whole cornea for proper utilization, such as transplantation. Previous reports have demonstrated the importance of proper endothelial maintenance during preservation for improved corneal function. As such, analyses into the modes of cell death will allow for future in-depth investigations of the complex stresses and re ...
DOI:
10.1016/j.cryobiol.2013.02.053

30. 5,5-Fused thiophene γ-lactams as templates for serine protease inhibition

Author:
Marie E. Migaud; Rupert C. Wilmouth; Gary I. Mills; Gareth J. Wayne; Catherine Risley; Christopher Chambers; Simon J. F. Macdonald; Christopher J. Schofield
Source:
Chemical communications 2002 v. no.12 pp. 1274-1275
ISSN:
1364-548X
Subject:
chemical reactions; enzyme inhibition; lactams; serine proteinases; thiophene
Abstract:
... Novel 5,5-fused thiophene lactams are potent inhibitors and acylating agents of HNE and PPE. ...
DOI:
10.1039/b111627d

31. 5-Methyl Furfural Reduces the Production of Malodors by Inhibiting Sodium l-Lactate Fermentation of Staphylococcus epidermidis: Implication for Deodorants Targeting the Fermenting Skin Microbiome

Author:
Manish Kumar; Binderiya Myagmardoloonjin; Sunita Keshari; Indira Putri Negari; Chun-Ming Huang
Source:
Microorganisms 2019 v.7 no.8 pp. -
ISSN:
2076-2607
Subject:
Staphylococcus epidermidis; acetolactate synthase; bacteria; carbon; colorimetry; deodorants; enzyme inhibition; fermentation; furfural; ingredients; lactic acid; metabolites; microbiome; off odors; skin (animal)
Abstract:
... Staphylococcus epidermidis (S. epidermidis) is a common bacterial colonizer on the surface of human skin. Lactate is a natural constituent of skin. Here, we reveal that S. epidermidis used sodium l-lactate as a carbon source to undergo fermentation and yield malodors detected by gas colorimetric tubes. Several furan compounds such as furfural originating from the fermentation metabolites play a ro ...
DOI:
10.3390/microorganisms7080239
PubMed:
31387211
PubMed Central:
PMC6723266

32. 5Α-Reductase inhibitory tannin-related compounds isolated from Shorea laeviforia

Author:
Yoshio Hirano; Ryuichiro Kondo; Kokki Sakai
Source:
Journal of wood science 2003 v.49 no.4 pp. 339-343
ISSN:
1435-0211
Subject:
NADP (coenzyme); Shorea; ellagic acid; enzyme activity; enzyme inhibition; enzymes; gallic acid; heartwood; liver; rats; testosterone
Abstract:
... Five tannin-related compounds – gallic acid, flavogallonic acid dilactone, valoneic acid dilactone, gallagyldilactone, ellagic acid – were isolated from the heartwood of Shorea laeviforia, and the inhibitory activity of each against rat liver 5Α-reductase was evaluated. Valoneic acid dilactone and gallagyldilactone exhibited positive inhibitory activity, but gallic acid and ellagic acid did not. F ...
DOI:
10.1007/s10086-002-0481-y

33. 5α-reductase inhibition and hair growth promotion of some Thai plants traditionally used for hair treatment

Author:
Naphatsorn Kumar; Wandee Rungseevijitprapa; Nual-Anong Narkkhong; Maitree Suttajit; Chaiyavat Chaiyasut
Source:
Journal of ethnopharmacology 2012 v.139 no.3 pp. 765-771
ISSN:
0378-8741
Subject:
Carthamus tinctorius; Phyllanthus emblica; alopecia; enzyme inhibition; ethanol; growth promotion; herbs; loss prevention; mechanism of action; mice; rats
Abstract:
... ETHNOPHARMACOLOGICAL RELEVANCE: Many Thai traditional herbs have been used for hundreds of years for hair treatment and nourishment, including hair loss. However, scientific evidence about their mechanisms of action has not yet been elucidated. AIMS OF THE STUDY: The purpose of this research is to define the possible mechanisms involved in hair loss treatment of the selected plants by determining ...
DOI:
10.1016/j.jep.2011.12.010
PubMed:
22178180

34. 62 Peripheral transcriptome response to embryo mortality in Holstein cows

Author:
C. L. Gonzalez-Berrios; H. M. Georges; J. V. Bishop; H. Van Campen; M. G. Thomas; T. R. Hansen
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 156
ISSN:
1031-3613
Subject:
CD4-positive T-lymphocytes; Holstein; adenylate cyclase; adhesion; calcium; conceptus; corpus luteum; cows; embryonic mortality; endometrium; enzyme inhibition; enzyme-linked immunosorbent assay; estradiol; estrous cycle; gene expression regulation; genes; immune response; interferons; messenger RNA; mitogen-activated protein kinase; pregnancy; semen; sequence analysis; steroidogenesis; transcriptome; tumor necrosis factors
Abstract:
... Exactly how failure through conceptus-endometrial signalling on Day 16 of pregnancy causes early embryo mortality (EM) is yet unknown. It was hypothesised that EM pregnancies are associated with an impaired endocrine action of interferon tau (IFNT) from the conceptus on the corpus luteum (CL) and peripheral blood mononuclear cells (PBMC), in addition to paracrine failure in preventing the upregula ...
DOI:
10.1071/RDv32n2Ab62

35. 69 Phosphorylation of mechanistic target of rapamycin in porcine blastocyst-stage embryos is dependent on the concentration of glutamine in the medium

Author:
P. Chen; L. Spate; R. Prather
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 160
ISSN:
1031-3613
Subject:
analysis of variance; antibodies; blastocyst; cell proliferation; culture media; embryo culture; enzyme inhibition; enzymes; glutamine; immunoblotting; leucine; membrane proteins; mitochondria; neoplasm cells; neoplasms; phosphorylation; rapamycin; swine; zygote
Abstract:
... Supplementation of glutamine to porcine embryo culture medium improves blastocyst development, increases leucine consumption, and enhances mitochondrial activity. In cancer cells, glutamine has been implicated in the phosphorylation and activation of mechanistic target of rapamycin (MTOR) to support rapid cellular proliferation. The objective of this study was to determine if phosphorylation of MT ...
DOI:
10.1071/RDv32n2Ab69

36. 6‐Gingerol inhibits fungal alpha amylase: Enzyme kinetic and molecular modeling studies

Author:
Ignatius Tintu; Kalarickal Vijayan Dileep; Chandran Remya; Anu Augustine; Chittalakkottu Sadasivan
Source:
Stärke = 2012 v.64 no.8 pp. 607-612
ISSN:
0038-9056
Subject:
alpha-amylase; animals; enzyme inhibition; fungi; industrial applications; models; reducing sugars; starch
Abstract:
... Alpha amylases are hydrolytic enzymes that are widespread in nature, being found in animals, microorganisms, and plants. These enzymes hydrolyze starch molecules into reducing sugars. Alpha amylases from microbial sources have several applications in industrial sectors. This enzyme is an effective target for the development of anti‐fungal agents. The binding affinity and enzyme inhibitory properti ...
DOI:
10.1002/star.201200004

37. 7-Acylamino-3H-1,2-benzoxathiepine 2,2-dioxides as new isoform-selective carbonic anhydrase IX and XII inhibitors

Author:
Aleksandrs Pustenko; Alessio Nocentini; Anastasija Balašova; Mikhail Krasavin; Raivis Žalubovskis; Claudiu T. Supuran
Source:
Journal of Enzyme Inhibition and Medicinal Chemistry 2020 v.35 no.1 pp. 650-656
ISSN:
1475-6366
Subject:
arthritis; carbonate dehydratase; chemical reactions; chemotypes; chlorides; enzyme inhibition; humans; ischemia; zinc
Abstract:
... A series of 3H-1,2-benzoxathiepine 2,2-dioxides incorporating 7-acylamino moieties were obtained by an original procedure starting from 5-nitrosalicylaldehyde, which was treated with propenylsulfonyl chloride followed by Wittig reaction of the bis-olefin intermediate. The new derivatives, belonging to the homosulfocoumarin chemotype, were assayed as inhibitors of the zinc metalloenzyme carbonic an ...
Handle:
10113/7345083
DOI:
10.1080/14756366.2020.1722658
CHORUS:
10.1080/14756366.2020.1722658

38. 7-Methylguanosine Diphosphate (m7GDP) Is Not Hydrolyzed but Strongly Bound by Decapping Scavenger (DcpS) Enzymes and Potently Inhibits Their Activity

Author:
Wypijewska Anna; Bojarska Elzbieta; Lukaszewicz Maciej; Stepinski Janusz; Jemielity Jacek; Davis Richard E.; Darzynkiewicz Edward
Source:
Biochemistry 2012 v.51 no.40 pp. 8003-8013
ISSN:
1520-4995
Subject:
Nematoda; binding capacity; biochemical pathways; enzyme inhibition; enzymes; humans; hydrolysis; messenger RNA; nucleosides; proteins; yeasts
Abstract:
... Decapping scavenger (DcpS) enzymes catalyze the cleavage of a residual cap structure following 3′ → 5′ mRNA decay. Some previous studies suggested that both m⁷GpppG and m⁷GDP were substrates for DcpS hydrolysis. Herein, we show that mononucleoside diphosphates, m⁷GDP (7-methylguanosine diphosphate) and m₃²,²,⁷GDP (2,2,7-trimethylguanosine diphosphate), resulting from mRNA decapping by the Dcp1/2 c ...
DOI:
10.1021/bi300781g
PubMed:
22985415
PubMed Central:
PMC3495079

39. 9,10-Phenanthrenequinone promotes secretion of pulmonary aldo-keto reductases with surfactant

Author:
Toshiyuki Matsunaga; Mariko Haga; Gou Watanabe; Yuhki Shinoda; Satoshi Endo; Yu Kajiwara; Hiroyuki Tanaka; Naoki Inagaki; Ossama El-Kabbani; Akira Hara
Source:
Cell and tissue research 2012 v.347 no.2 pp. 407-417
ISSN:
0302-766X
Subject:
apoptosis; culture media; enzyme inhibition; epithelium; humans; oxidoreductases; pulmonary alveoli; quinones; rats; reactive oxygen species; secretion; surfactants; toxicity; viability
Abstract:
... 9,10-Phenanthrenequinone (9,10-PQ), a major quinone in diesel exhaust particles, induces apoptosis via the generation of reactive oxygen species (ROS) because of 9,10-PQ redox cycling. We have found that intratracheal infusion of 9,10-PQ facilitates the secretion of surfactant into rat alveolus. In the cultured rat lung, treatment with 9,10-PQ results in an increase in a lower-density surfactant b ...
DOI:
10.1007/s00441-011-1304-5
PubMed:
22281686

40. 9-Azido-9-deoxy-2,3-difluorosialic Acid as a Subnanomolar Inhibitor against Bacterial Sialidases

Author:
Wanqing Li; Abhishek Santra; Hai Yu; Teri J. Slack; Musleh M. Muthana; Dashuang Shi; Yang Liu; Xi Chen
Source:
Journal of organic chemistry 2019 v.84 no.11 pp. 6697-6708
ISSN:
1520-6904
Subject:
Clostridium perfringens; Escherichia coli; Influenza A virus; Pasteurella multocida; Vibrio cholerae; acids; chemical structure; derivatization; enzyme inhibition; enzyme inhibitors; fluorine; fructose-bisphosphate aldolase; humans; organic chemistry; sialic acid; sialidase; stereochemistry
Abstract:
... A library of 2(a),3(a/e)-difluorosialic acids and their C-5 and/or C-9 derivatives were chemoenzymatically synthesized. Pasteurella multocida sialic acid aldolase (PmAldolase), but not its Escherichia coli homologue (EcAldolase), was found to catalyze the formation of C5-azido analogue of 3-fluoro(a)-sialic acid. In comparison, both PmAldolase and EcAldolase could catalyze the synthesis of 3-fluor ...
DOI:
10.1021/acs.joc.9b00385
PubMed:
31083938
PubMed Central:
PMC6660241

41. 90-day subchronic toxicity study and reproductive toxicity studies on ACE-inhibiting lactotripeptide

Author:
Dent, M.P.; O'Hagan, S.; Braun, W.H.; Schaetti, P.; Marburger, A.; Vogel, O.
Source:
Food and chemical toxicology 2007 v.45 no.8 pp. 1468-1477
ISSN:
0278-6915
Subject:
renin-angiotensin system; valine; diastolic blood pressure; subchronic toxicity; angiotensin I; proline; systolic blood pressure; milk; isoleucine; casein; reproductive toxicology; kidneys; toxicity testing; enzyme inhibition; health foods; tripeptides
DOI:
10.1016/j.fct.2007.02.006

42. Phaseolus vulgaris L. Extract: Alpha-Amylase Inhibition against Metabolic Syndrome in Mice

Author:
Laura Micheli; Elena Lucarini; Elena Trallori; Carmen Avagliano; Carmen De Caro; Roberto Russo; Antonio Calignano; Carla Ghelardini; Alessandra Pacini; Lorenzo Di Cesare Mannelli
Source:
Nutrients 2019 v.11 no.8 pp. -
ISSN:
2072-6643
Subject:
NAD (coenzyme); NADH dehydrogenase; Phaseolus vulgaris; alpha-amylase; aorta; atorvastatin; blood glucose; body weight; catalase; cholesterol; enzyme activity; enzyme inhibition; enzyme inhibitors; fatty liver; glucose; glutathione-disulfide reductase; heart; high fat diet; histology; insulin; kidney beans; lipid peroxidation; liver; metabolic syndrome; metformin; mice; models; phytochemicals; therapeutics; triacylglycerols
Abstract:
... To examine the effects of the alpha-amylase inhibitor isoform 1 called phaseolamin, a standardized extract from white kidney beans (Phaseolus vulgaris L.) was tested against the hallmarks of metabolic syndrome. The efficacy of a per os repeated treatment with P. vulgaris extract (500 mg/kg) was compared with metformin (100 mg/kg) and atorvastatin (10 mg/kg) in a model of metabolic syndrome evoked ...
DOI:
10.3390/nu11081778
PubMed:
31374931
PubMed Central:
PMC6723332

43. Salix tetrasperma Roxb. Extract Alleviates Neuropathic Pain in Rats via Modulation of the NF-κB/TNF-α/NOX/iNOS Pathway

Author:
Mansour Sobeh; Mona F. Mahmoud; Samar Rezq; Amira E. Alsemeh; Omar M. Sabry; Islam Mostafa; Mohamed A. O. Abdelfattah; Khadija Ait El-Allem; Assem M. El-Shazly; Aziz Yasri; Michael Wink
Source:
Antioxidants 2019 v.8 no.10 pp. -
ISSN:
2076-3921
Subject:
NAD(P)H oxidase (H2O2-forming); Salix; Schwann cells; animal models; antioxidant activity; biomarkers; brain stem; burning; dose response; enzyme inhibition; enzyme-linked immunosorbent assay; flowers; high performance liquid chromatography; inducible nitric oxide synthase; inflammation; methanol; myelin sheath; nerve tissue; oxidative stress; pain; patients; prostaglandin synthase; prostaglandins; rats; secondary metabolites; somatosensory disorders; tandem mass spectrometry; traditional medicine; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Patients with neuropathic pain experience chronic painful tingling, burning, and prickling sensations accompanied with hyperalgesia and/or allodynia. In this study, 38 secondary metabolites of a methanol extract from Salix tetrasperma flowers were identified by liquid chromatography-mass spectrometry (HPLC-MS/MS). The extract showed substantial anti-inflammatory, central and peripheral anti-nocice ...
DOI:
10.3390/antiox8100482
PubMed:
31614846
PubMed Central:
PMC6826723

44. ABA signal transduction from ABA receptors to ion channels

Author:
Chae Woo Lim; Woonhee Baek; Sohee Lim; Sung Chul Lee
Source:
Genes & genomics 2012 v.34 no.4 pp. 345-353
ISSN:
1976-9571
Subject:
abiotic stress; abscisic acid; dephosphorylation; electrophysiology; enzyme inhibition; gene expression; genes; ion channels; pathogens; phosphoprotein phosphatase; protein kinases; receptors; seed dormancy; seed germination; seedlings; signal transduction; stomatal movement; stress response
Abstract:
... The plant hormone abscisic acid (ABA) is involved in regulating a number of major processes such as seed dormancy, seedling development, and biotic and abiotic stress responses. The function and effect of ABA on pathogens are still unclear, but the roles of ABA in seed germination and abiotic stress responses have been well characterized. Abiotic stresses elevate ABA levels and activate ABA signal ...
DOI:
10.1007/s13258-012-0081-1

45. ACAT inhibition of alkamides identified in the fruits of Piper nigrum

Author:
Rho, M.C.; Lee, S.W.; Park, H.R.; Choi, J.H.; Kang, J.Y.; Kim, K.; Lee, H.S.; Kim, Y.K.
Source:
Phytochemistry 2007 v.68 no.6 pp. 899-903
ISSN:
0031-9422
Subject:
humans; amides; cholesterol acyltransferase; cultured cells; spectral analysis; rats; enzyme inhibition; chemical structure; Piper nigrum
Abstract:
... In this study, via a bioactivity-guided fractionation of MeOH extracts of the fruits of Piper nigrum, alkamide (5) and five previously-identified alkamides were isolated. Their structures were elucidated via spectroscopic analysis (1H, 13C NMR and ESI-MS), as follows: retrofractamide A (1), pipercide (2), piperchabamide D (3), pellitorin (4), dehydroretrofractamide C (5) and dehydropipernonaline ( ...
DOI:
10.1016/j.phytochem.2006.11.025
PubMed:
17188313

46. ACE inhibitory tetrapeptides from Amaranthus hypochondriacus 11S globulin

Author:
Vecchi, B.; Anon, M.C.
Source:
Phytochemistry 2009 v.70 no.7 pp. 864-870
ISSN:
0031-9422
Subject:
Amaranthus hypochondriacus; peptides; globulins; antihypertensive effect; peptidyl-dipeptidase A; enzyme inhibition; molecular models; amino acid sequences
Abstract:
... Amaranth seed is a valuable source of dietary protein with very high nutritional quality, and recently its potential as a nutraceutical has been proposed. The aim of this work was to provide experimental evidence for the presence of anti-hypertensive peptides in globulin 11S, one of the major constituents of the seed, by means of an in-silico based peptide library screening method. A three-dimensi ...
DOI:
10.1016/j.phytochem.2009.04.006
PubMed:
19443002

47. ACE-Inhibitory and Radical-Scavenging Activity of Peptides Derived from β-Lactoglobulin f(19-25). Interactions with Ascorbic Acid

Author:
Hernandez-Ledesma, B.; Amigo, L.; Recio, I.; Bartolome, B.
Source:
Journal of agricultural and food chemistry 2007 v.55 no.9 pp. 3392-3397
ISSN:
0021-8561
Subject:
free radicals; peptidyl-dipeptidase A; peptides; enzyme inhibitors; protein conformation; antioxidant activity; enzyme inhibition; beta-lactoglobulin; antioxidants
Abstract:
... In this work, the angiotensin-converting enzyme (ACE)-inhibitory and radical-scavenging activities of the β-lactoglobulin (β-Lg)-derived peptides WY f(19-20), WYS f(19-21), WYSL f(19-22), WYSLA f(19-23), WYSLAM f(19-24), and WYSLAMA f(19-25) have been determined. The ACE-inhibitory activity (IC50) varied from 38.3 to 90.4 micromolar, with the exception of WYS (>500 micromolar). All β-Lg-derived pe ...
DOI:
10.1021/jf063427j

48. ACE-inhibitory activity and structural properties of peptide Asp-Lys-Ile-His-Pro [beta-CN f(47-51)]. Study of the peptide forms synthesized by different methods

Author:
Gomez-Ruiz, J.A.; Recio, I.; Belloque, J.
Source:
Journal of agricultural and food chemistry 2004 v.52 no.20 pp. 6315-6319
ISSN:
0021-8561
Subject:
oligopeptides; synthetic peptides; enzyme inhibitors; enzyme inhibition; peptidases; food chemistry; functional foods
Abstract:
... Some of the most potent ACE-inhibitory peptides described in food have a proline at the end of their sequence, a characteristic that can cause problems in the synthesis procedures. In this work, we studied two different preparations of peptide Asp-Lys-Ile-His-Pro (DKIHP), which were obtained by two different synthetic procedures (Boc and Fmoc). The peptide synthesized by the Boc method yielded a u ...
DOI:
10.1021/jf049532f

49. ACE-inhibitory activity of probiotic yoghurt

Author:
Donkor, O.N.; Henriksson, A.; Singh, T.K.; Vasiljevic, T.; Shah, N.P.
Source:
International dairy journal 2007 v.17 no.11 pp. 1321-1331
ISSN:
0958-6946
Subject:
Lactobacillus delbrueckii subsp. bulgaricus; cold storage; peptidyl-dipeptidase A; Bifidobacterium animalis; peptides; antihypertensive effect; storage quality; amino acid sequences; Lactobacillus casei; yogurt; enzyme activity; enzyme inhibition; Lactobacillus acidophilus; Streptococcus thermophilus
Abstract:
... In this study, the in vitro angiotensin-converting enzyme (ACE)-inhibitory (ACE-I) activity of peptide fractions from different yoghurt batches was assessed. Inhibition of ACE activity resulted in an overall antihypertensive effect. Yoghurts were prepared either using a sole yoghurt culture including Lactobacillus delbrueckii ssp. bulgaricus Lb1466 and Streptococcus thermophilus St1342, or L. acid ...
DOI:
10.1016/j.idairyj.2007.02.009

50. ACE-inhibitory activity of tilapia protein hydrolysates

Author:
Raghavan, Sivakumar; Kristinsson, Hordur G.
Source:
Food chemistry 2009 v.117 no.4 pp. 582-588
ISSN:
0308-8146
Subject:
peptidyl-dipeptidase A; enzyme inhibition; enzyme activity; tilapia (common name); food processing wastes; protein hydrolysates; fractionation; ultrafiltration; separation
Abstract:
... Fish processing wastes can be used for preparing bioactive peptides with various functionalities. Our objective was to evaluate the in vitro angiotensin converting enzyme (ACE) inhibitory activity of tilapia protein hydrolysates and its corresponding fractionates. Tilapia protein was alkali-solubilised at pH 11.0 and recovered at pH 5.5 to obtain a stable substrate. This substrate was hydrolysed u ...
DOI:
10.1016/j.foodchem.2009.04.058

51. ACER3 supports development of acute myeloid leukemia

Author:
Chen Chen; Yancun Yin; Chunling Li; Jinliang Chen; Jingjing Xie; Zhigang Lu; Minjing Li; Yuesi Wang; Cheng Cheng Zhang
Source:
Biochemical and biophysical research communications 2016 v.478 no.1 pp. 33-38
ISSN:
0006-291X
Subject:
Lentivirus; apoptosis; cell growth; databases; enzyme inhibition; humans; myeloid leukemia; neoplasm cells; pathogenesis; patients; therapeutics
Abstract:
... No new therapy for acute myeloid leukemia (AML) has been approved for more than 30 years. To effectively treat AML, new molecular targets and therapeutic approaches must be identified. In silico analysis of several databases of AML patients demonstrated that the expression of alkaline ceramidase 3 (ACER3) significantly inversely correlates with the overall survival of AML patients. To determine wh ...
DOI:
10.1016/j.bbrc.2016.07.099

52. ALCAPs induce mitochondrial apoptosis and activate DNA damage response by generating ROS and inhibiting topoisomerase I enzyme activity in K562 leukemia cell line

Author:
Nuray Bogurcu; Canan Sevimli-Gur; Besra Ozmen; Erdal Bedir; Kemal Sami Korkmaz
Source:
Biochemical and biophysical research communications 2011 v.409 no.4 pp. 738-744
ISSN:
0006-291X
Subject:
Alkanna; DNA damage; DNA topoisomerase; antioxidants; apoptosis; caspase-3; cell growth; doxorubicin; enzyme activity; enzyme inhibition; flow cytometry; landscapes; leukemia; neoplasm cells; phosphorylation; plasmids; therapeutics
Abstract:
... Endemic Alkanna cappadocica was used to isolate novel antitumor molecules from Turkish landscapes in our previous studies. In this study, deoxyalkannin (ALCAP1), β,β-dimethylacrylalkannin (ALCAP2), acetylalkannin (ALCAP3), and alkannin (ALCAP4) as well as the novel isolated compounds 5-methoxydeoxyalkannin (ALCAP5), 8-methoxydeoxyalkannin (ALCAP6), 5-methoxyacetylalkannin (ALCAP7), 5-methoxy-β,β-d ...
DOI:
10.1016/j.bbrc.2011.05.078

53. AMP-activated protein kinase and adipogenesis in sheep fetal skeletal muscle and 3T3-L1 cells

Author:
Tong, J.; Zhu, M.J.; Underwood, K.R.; Hess, B.W.; Ford, S.P.; Du, M.
Source:
Journal of animal science 2008 v.86 no.6 pp. 1296-1305
ISSN:
0021-8812
Subject:
adipocytes; transcription factors; muscle development; marbling; sheep; cell culture; skeletal muscle; fetal development; gene expression; cAMP-dependent protein kinase; lipogenesis; fetus; maternal nutrition; adenosine monophosphate; enzyme activity; enzyme inhibition
Abstract:
... Marbling, or i.m. fat, is an important factor determining beef quality. Both adipogenesis and hypertrophy of existing adipocytes contribute to enhanced marbling. We hypothesized that the fetal stage is important for the formation of i.m. adipocytes and that AMP-activated protein kinase (AMPK) has a key role in adipogenesis during this stage. The objective of this study was to assess the role of AM ...
DOI:
10.2527/jas.2007-0794
PubMed:
18344293

54. An AOP-based alternative testing strategy to predict the impact of thyroid hormone disruption on swim bladder inflation in zebrafish

Author:
Evelyn Stinckens; Lucia Vergauwen; Gerald T. Ankley; Ronny Blust; Veerle M. Darras; Daniel L. Villeneuve; Hilda Witters; David C. Volz; Dries Knapen
Source:
Aquatic toxicology 2018 v.200 pp. 1-12
ISSN:
0166-445X
Subject:
Danio rerio; adverse outcome pathways; data collection; embryo (animal); enzyme inhibition; enzymes; fish; prediction; swim bladder; thyroid hormones; toxicity; toxicity testing
Abstract:
... The adverse outcome pathway (AOP) framework can be used to help support the development of alternative testing strategies aimed at predicting adverse outcomes caused by triggering specific toxicity pathways. In this paper, we present a case-study demonstrating the selection of alternative in chemico assays targeting the molecular initiating events of established AOPs, and evaluate use of the resul ...
DOI:
10.1016/j.aquatox.2018.04.009
PubMed:
29702435
PubMed Central:
PMC6002951

55. APPL1 knockdown blocks adipogenic differentiation and promotes adipocyte lipolysis

Author:
Zhongyuan Wen; Zhao Tang; Mingxin Li; Yemin Zhang; Junfeng Li; Yingkang Cao; Deling Zhang; Yalin Fu; Changhua Wang
Source:
Molecular and cellular endocrinology 2020 v.506 pp. 110755
ISSN:
0303-7207
Subject:
adipocytes; adipogenesis; adiponectin; autophagy; body fat distribution; brown adipose tissue; cAMP-dependent protein kinase; enzyme inhibition; heat production; insulin; insulin resistance; lipolysis; noninsulin-dependent diabetes mellitus; obesity; peroxisome proliferator-activated receptor gamma; proteasome inhibitors; protein content
Abstract:
... Adipocyte dysfunction is closely associated with the development of obesity, insulin resistance, and type 2 diabetes. In addition to having a positive effect on adiponectin pathway and insulin signaling through direct and/or indirect mechanisms, adapter protein APPL1 has also been reported to regulate body weight, brown fat tissues thermogenesis, and body fat distribution in diabetic individuals. ...
DOI:
10.1016/j.mce.2020.110755

56. ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition

Author:
Jiaqi Chu; Yuanyuan Fu; Jiecheng Xu; Xueping Zheng; Qianqian Gu; Xia Luo; Qi Dai; Shuxian Zhang; Peiqing Liu; Liang Hong; Min Li
Source:
Archives of biochemistry and biophysics 2018 v.644 pp. 29-36
ISSN:
0003-9861
Subject:
autophagy; enzyme inhibition; inhibitory concentration 50; proteins
Abstract:
... Atg4 is essential for autophagosome formation and Atg8 recycle with the function of processing the precursor and the lipidated Atg8-family proteins. Abnormal autophagic activity is involved in a variety of pathophysiological diseases and ATG4B is of interest as a potential therapeutic target due to its key roles in autophagy process. So ATG4B inhibitors are highly needed. FMK-9a is the most potent ...
DOI:
10.1016/j.abb.2018.03.001

57. ATP-Dependent Inactivation of Escherichia coli γ-Glutamylcysteine Synthetase by L-Glutamic Acid γ-Monohydroxamate

Author:
Makoto KATOH; Jun HIRATAKE; Jun’ichi ODA
Source:
Bioscience, biotechnology, and biochemistry 1998 v.62 no.7 pp. 1455-1457
ISSN:
1347-6947
Subject:
Escherichia coli; adenosine triphosphate; aspartic acid; enzyme activation; enzyme activity; enzyme inactivation; enzyme inhibition; glutamate-cysteine ligase; glutamic acid; hydroxamic acids
Abstract:
... Incubation of Escherichia coli γ-glutamylcysteine synthetase with L-glutamic acid γ-monohydroxamate and ATP caused slow but irreversible inhibition of the enzyme, and more than 90% activity was lost in three days. The enzyme was not inactivated when ATP was absent or L-aspartic acid β-monohydroxamate was substituted for L-glutamic acid γ-monohydroxamate, suggesting that the inactivation process re ...
DOI:
10.1271/bbb.62.1455

58. ATR kinase regulates its attenuation via PPM1D phosphatase recruitment to chromatin during recovery from DNA replication stress signalling

Author:
Debadrita Bhattacharya; Disha Hiregange; Basuthkar J Rao
Source:
Journal of biosciences 2018 v.43 no.1 pp. 25-47
ISSN:
0250-5991
Subject:
DNA damage; DNA replication; cell cycle; cell nucleolus; chromatin; cytoplasm; enzyme activity; enzyme inhibition; eukaryotic cells; mortality; phosphorylation
Abstract:
... In eukaryotes, in response to replication stress, DNA damage response kinase, ATR is activated, whose signalling abrogation leads to cell lethality due to aberrant fork remodelling and excessive origin firing. Here we report that inhibition of ATR kinase activity specifically during replication stress recovery results in persistent ATR signalling, evidenced by the presence of ATR-dependent phospho ...
DOI:
10.1007/s12038-018-9736-7

59. Abeliophyllum distichum Nakai alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro

Author:
Eun-Young Kim; Jae-Hyun Kim; Minsun Kim; Jae Ho Park; Youngjoo Sohn; Hyuk-Sang Jung
Source:
Journal of ethnopharmacology 2020 v.257 pp. 112828
ISSN:
0378-8741
Subject:
Abeliophyllum distichum; acetates; actin; animal models; animal ovaries; antioxidants; bone resorption; diarrhea; dose response; enzyme inhibition; female genital diseases; femur; genes; indigenous species; leaves; mitogen-activated protein kinase; morphometry; oral administration; osteoporosis; ovariectomy; postmenopause; protective effect; rats; traditional medicine; women; South Korea
Abstract:
... Abeliophyllum distichum Nakai (AD), called Miseon, is one of Korea's monotypic endemic species. As a folk remedy, the AD has been used to treat inflammatory disease, stomachaches, diarrhea, and gynecologic disease in Korea. Some researchers have reported that the AD has anti-cancer, anti-inflammatory, and anti-oxidant effects. But the protective effect of AD leaf for osteoporosis has not been repo ...
DOI:
10.1016/j.jep.2020.112828

60. Abrus agglutinin inhibits oral carcinogenesis through inactivation of NRF2 signaling pathway

Author:
Debasna P. Panigrahi; Chandra S. Bhol; Nivetha R; Siddavaram Nagini; Shankargouda Patil; Tapas K. Maiti; Sujit K. Bhutia
Source:
International journal of biological macromolecules 2020 v.155 pp. 1123-1132
ISSN:
0141-8130
Subject:
Abrus precatorius; agglutinins; animal disease models; antineoplastic activity; antioxidant enzymes; apoptosis; autophagy; carcinogenesis; catalase; enzyme activity; enzyme inhibition; glutathione peroxidase; immunohistochemistry; mouth neoplasms; seeds; signal transduction; superoxide dismutase; transcription factors
Abstract:
... Abrus agglutinin (AGG), a heterotetrameric type II ribosome inactivating protein isolated from the seeds of Abrus precatorius shows potent antitumor activity in different cancer models. We examined the role of antioxidant system in modulation of the anticancer activity of AGG in in vitro and in hamster model of oral cancer. AGG promotes apoptosis through accumulation of ROS in CAL33 cells. Interes ...
DOI:
10.1016/j.ijbiomac.2019.11.079

61. Abscisic acid-induced hydrogen peroxide is required for anthocyanin accumulation in leaves of rice seedlings

Author:
Hung, Kuo Tung; Cheng, Deng Guo; Kao, Ching Huei
Source:
Journal of plant physiology 2008 v.165 no.12 pp. 1280-1287
ISSN:
0176-1617
Subject:
Oryza sativa; rice; seedlings; abscisic acid; hydrogen peroxide; anthocyanins; phosphatidylinositol 3-kinase; enzyme inhibition
Abstract:
... The role of hydrogen peroxide (H2O2) in abscisic acid (ABA)-induced anthocyanin accumulation in detached and intact leaves of rice seedlings was investigated. Treatment with ABA resulted in an accumulation of anthocyanins in detached rice leaves. Dimethylthiourea, a chemical trap for H2O2, was observed to be effective in inhibiting ABA-induced accumulation of anthocyanins. Inhibitors of NADPH oxid ...
DOI:
10.1016/j.jplph.2007.10.008

62. Ac-tLeu-Asp-H is the minimal and highly effective human caspase-3 inhibitor: biological and in silico studies

Author:
Anna Ferrucci; Loris Leboffe; Mariangela Agamennone; Antonella Di Pizio; Marco Fiocchetti; Maria Marino; Paolo Ascenzi; Grazia Luisi
Source:
Amino acids 2015 v.47 no.1 pp. 153-162
ISSN:
0939-4451
Subject:
NAD ADP-ribosyltransferase; adenocarcinoma; aldehydes; apoptosis; caspase-3; colon; enzyme inhibition; humans; membrane permeability; pH; proteins; proteolysis
Abstract:
... Caspase-3 displays a pivotal role as an executioner of apoptosis, hydrolyzing several proteins including the nuclear enzyme poly(ADP-ribose)polymerase (PARP). Ac-Asp-Glu-Val-Asp-H (Kᵢ° = 2.3 × 10⁻¹⁰M at pH 7.5 and 25.0 °C), designed on the basis of the cleavage site of PARP, has been reported as a highly specific human caspase-3 inhibitor. Here, di- and tri-peptidyl aldehydes 11–13 and 27–29 have ...
DOI:
10.1007/s00726-014-1855-3

63. Acaricidal activity and enzyme inhibitory activity of active compounds of essential oils against Psoroptes cuniculi

Author:
Xiao-Fei Shang; Li-Xia Dai; Ying-Qian Liu; Zhong-Min Zhao; Jun-Cai Li; Guan-Zhou Yang; Cheng-Jie Yang
Source:
Veterinary parasitology 2019 v.267 pp. 54-59
ISSN:
0304-4017
Subject:
Psoroptes cuniculi; acaricidal properties; acaricides; acetylcholinesterase; active ingredients; cytochrome P-450; cytotoxicity; enzyme inhibition; essential oils; eugenol; glutathione transferase; hepatocytes; humans; industry; inhibitory concentration 50; keratinocytes; lethal concentration 50; liver; mechanism of action; medicinal properties; mites; rabbits; skin irritation
Abstract:
... Plant essential oils and its chemical compositions are commonly applied in medicinal and other industries due to their broad advanced pharmacological activities. In the present study, we systematically evaluated the acaricidal activities of twelve compounds of essential oils against Psoroptes cuniculi in vitro and in vivo. In addition, to support the clinic uses, their toxicities against immortali ...
DOI:
10.1016/j.vetpar.2019.01.013

64. Accase-inhibitor Herbicide Resistance in Downy Brome (Bromus Tectorum) in Oregon

Author:
Ball Daniel A.; Frost Sandra M.; Bennett Larry H.
Source:
Weed science 2007 v.55 no.2 pp. 91-94
ISSN:
0043-1745
Subject:
herbicide resistance; Bromus tectorum; fluazifop-P; acetyl-CoA carboxylase; enzyme inhibition; grass weeds; application rate; quizalofop; sethoxydim; clethodim; herbicide-resistant weeds; greenhouse experimentation; crop-weed competition; Festuca rubra; seed crop production; Oregon
Abstract:
... In spring 2005, a downy brome population with possible resistance to fluazifop-P, an acetyl-CoA carboxylase (ACCase) inhibitor (group 1) herbicide was found in a commercial creeping red fescue seed production field, near La Grande, OR, where fluazifop-P had been used to control downy brome repeatedly over 7 yr. Greenhouse experiments were conducted to confirm resistance to a number of group 1 herb ...
DOI:
10.1614/WS-06-120.1

65. Acceptability of alginate enriched bread and its effect on fat digestion in humans

Author:
David Houghton; Matthew D. Wilcox; Iain A. Brownlee; Peter I. Chater; Chris J. Seal; Jeffrey P. Pearson
Source:
Food hydrocolloids 2019 v.93 pp. 395-401
ISSN:
0268-005X
Subject:
adverse effects; alginates; breads; carboxylic ester hydrolases; compliance; enzyme inhibition; food records; humans; hydrocolloids; ileum; lifestyle; lipid content; lipolysis; obesity; physical activity; questionnaires; triacylglycerols
Abstract:
... Lifestyle interventions and physical activity remain the cornerstone of obesity management, as pharmacological therapies (orlistat) are associated with gastrointestinal (GI) side effects. Combining orlistat with fibers can reduce side effects, improving compliance. Therefore, a fiber that inhibits lipase without side effects could help treat obesity.The aims of the present work were to assess whet ...
DOI:
10.1016/j.foodhyd.2019.02.027
PubMed:
32226189
PubMed Central:
PMC7086458

66. Accumulation of betacyanin in Hylocereus undatus rind: Pigment stability analysis and its role in xanthine oxidase inhibition

Author:
Diksha Dey; Hridya Hemachandran; Thirumal Kumar D; George Priya Doss; Rossyda Priyadarshini; Ramamoorthy Siva
Source:
Phytomedicine plus 2022 v.2 no.1 pp. 100197
ISSN:
2667-0313
Subject:
Hylocereus undatus; active sites; amino acids; ascorbic acid; betalains; encapsulation; enzyme inhibition; fluorescence; nitrogen; pH; photophase; starch; temperature; uric acid; vacuoles; water solubility; xanthine oxidase
Abstract:
... Betalain pigments are water soluble natural pigments that contain nitrogen. Betacyanin have been reported to possess several biological properties In the present work, the pigment from the rind of Hylocereus undatus was analyzed for its stability upon elevated temperature, pH, time, light period, and metal ions. Further, the effect of betacyanin pigment on xanthine oxidase enzyme inhibition was st ...
DOI:
10.1016/j.phyplu.2021.100197

67. Accumulation of free tryptophan in azuki bean (Vigna angularis) induced by expression of a gene (OASA1D) for a modified α-subunit of rice anthranilate synthase

Author:
Hanafy, M.S.; Rahman, S.M.; Khalafalla, M.M.; El-Shemy, H.A.; Nakamoto, Y.; Ishimoto, M.; Wakasa, K.
Source:
Plant science 2006 v.171 no.6 pp. 670-676
ISSN:
0168-9452
Subject:
Oryza sativa; rice; plant proteins; oxo-acid-lyases; transgenes; enzyme inhibition; genetic transformation; gene expression regulation; enzyme activity; Vigna angularis var. angularis; transgenic plants; seeds; tryptophan; free amino acids; essential amino acids; biosynthesis; biochemical pathways; anthranilic acid; plant biochemistry
DOI:
10.1016/j.plantsci.2006.06.016

68. Accumulation of p-hydroxybenzoic acid in hairy roots of Daucus carota 2: Confirming biosynthetic steps through feeding of inhibitors and precursors

Author:
Sircar, Debabrata; Mitra, Adinpunya
Source:
Journal of plant physiology 2009 v.166 no.13 pp. 1370-1380
ISSN:
0176-1617
Subject:
phenylpropanoids; root systems; phenylalanine ammonia-lyase; coumaric acids; coumarate-CoA ligase; Daucus carota; lignin; coenzyme A; biochemical pathways; plant response; trans-cinnamate 4-monooxygenase; enzyme activity; enzyme inhibition
Abstract:
... Biosynthesis of hydroxybenzoates even at enzymatic level is poorly understood. In this report, effect of feeding of putative biosynthetic precursors and pathway-specific enzyme inhibitors of early phenylpropanoid pathway on p-hydroxybenzoic acid accumulation in chitosan-elicited hairy roots of Daucus carota was studied. Three selective metabolic inhibitors of plant phenylpropanoid pathway, namely, ...
DOI:
10.1016/j.jplph.2009.02.006
PubMed:
19342120

69. Accumulation of trichothecenes in liquid cultures of a Fusarium sporotrichioides mutant lacking a functional trichothecene C-15 hydroxylase

Author:
McCormick, S.P.; Hohn, T.M.
Source:
Applied and environmental microbiology 1997 v.63 no.5 pp. 1685-1688
ISSN:
0099-2240
Subject:
Fusarium sporotrichioides; mutants; strains; genes; enzyme inhibition; trichothecenes; biosynthesis; in vitro culture
Abstract:
... A mutant strain of Fusarium sporotrichioides NRRL 3299 produced by disruption of Tri11, a gene encoding a cytochrome P-450 monooxygenase, was shown to be altered in its ability to biosynthesize T-2 toxin. This mutant strain produced four trichothecenes that were not observed in cultures of the parent strain. The compounds were identified as isotrichodermin, 8-hydroxyisotrichodermin, 8-hvdroxyisotr ...
Handle:
10113/26569
PubMed:
16535589

70. Ace Inhibition and Enzymatic Resistance In Vitro of a Casein Hydrolysate Subjected to Plastein Reaction in the Presence of Extrinsic Proline and Ethanol- or Methanol-Water Fractionation

Author:
Hui Sun; Tie-Jing Li; Xin-Huai Zhao
Source:
International journal of food properties 2014 v.17 no.2 pp. 386-398
ISSN:
1094-2912
Subject:
casein; casein hydrolysates; enzyme inhibition; food quality; fractionation; hydrolysates; hydrolysis; peptides; plasteins; proline; solvents; subtilisin; temperature
Abstract:
... Casein was hydrolyzed by alcalase to a degree of hydrolysis of 10.9% to obtain a hydrolysate having ACE-inhibition in vitro with an IC ₅₀ value of 52.6 μ g/mL. The prepared hydrolysate was modified by alcalase-catalyzed plastein reaction with extrinsic proline added at 0.4 mol/mol free amino groups (on the basis of the hydrolysate), and fractionated by ethanol- or methanol-water solvents in propor ...
DOI:
10.1080/10942912.2011.642048

71. Acetic acid in aged vinegar affects molecular targets for thrombus disease management

Author:
Li Jing; Zhang Yanyan; Fan Junfeng
Source:
Food & function 2015 v.6 no.8 pp. 2845-2853
ISSN:
2042-650X
Subject:
acetic acid; adenosine diphosphate; arachidonic acid; citric acid; collagen; crosslinking; disease control; enzyme inhibition; fibrin; formic acid; lactic acid; malic acid; plasmin; plasminogen; platelet aggregation; prostaglandin synthase; thrombin; thrombosis; u-plasminogen activator; vinegars
Abstract:
... To elucidate the mechanism underlying the action of dietary vinegar on antithrombotic activity, acetic acid, the main acidic component of dietary vinegar, was used to determine antiplatelet and fibrinolytic activity. The results revealed that acetic acid significantly inhibits adenosine diphosphate (ADP)-, collagen-, thrombin-, and arachidonic acid (AA)-induced platelet aggregation. Acetic acid (2 ...
DOI:
10.1039/c5fo00327j

72. Acetolactate Synthase (ALS) Target-Site Mutations in ALS Inhibitor-Resistant Russian Thistle (Salsola tragus)

Author:
Warwick Suzanne I.; Sauder Connie A.; Beckie Hugh J.
Source:
Weed science 2010 v.58 no.3 pp. 244-251
ISSN:
0043-1745
Subject:
Salsola tragus; weed control; thifensulfuron; tribenuron; application rate; herbicide-resistant weeds; weed biology; acetolactate synthase; enzyme inhibition; plant breeding; site-directed mutagenesis; biotypes; mutants; genetic variation; genetic resistance; genes; nucleotide sequences; DNA fingerprinting; gene segregation; heterozygosity; polyploidy; Alberta; Saskatchewan
Abstract:
... ALS inhibitor-resistant biotypes are the fastest growing class of herbicide-resistant (HR) weeds. A Canadian ALS inhibitor-resistant biotype of Russian thistle was first reported in 1989. The molecular basis for ALS-inhibitor resistance is unknown for Canadian populations of this polyploid weed species, and was determined in this study for one Alberta and two Saskatchewan HR Russian thistle popula ...
DOI:
10.1614/WS-D-09-00083.1

73. Acetophenone derivatives from Acronychia pedunculata

Author:
Su, C.R.; Kuo, P.C.; Wang, M.L.; Liou, M.J.; Damu, A.G.; Wu, T.S.
Source:
Journal of natural products 2003 v.66 no.7 pp. 990-993
ISSN:
0163-3864
Subject:
protein-tyrosine kinases; ketones; stems; phytochemicals; enzyme inhibition; medicinal plants; plant extracts; chemical structure; enzyme inhibitors; carbon; isopropyl alcohol; antioxidants; spectral analysis; protons; bark; roots; Acronychia; Taiwan
DOI:
10.1021/np030054x
PubMed:
12880321

74. Acetylcholine Esterase Antibodies on BiOI Nanoflakes/TiO2 Nanoparticles Electrode: A Case of Application for General Photoelectrochemical Enzymatic Analysis

Author:
Zhao Wei-Wei; Shan Shu; Ma Zheng-Yuan; Wan Lin-Na; Xu Jing-Juan; Chen Hong-Yuan
Source:
Analytical chemistry 2013 v.85 no.24 pp. 11686-11690
ISSN:
1520-6882
Subject:
acetylcholinesterase; antibodies; biosensors; electrodes; enzyme inhibition; nanoparticles
Abstract:
... To date, almost all the established photoelectrochemical (PEC) enzymatic biosensors require the surface-confinement procedure to immobilize enzyme as biorecogniton element for probing various analytes of interest. This Letter develops a novel example without such necessity. Specifically, we first prepared a BiOI nanoflakes (NFs)/TiO₂ nanoparticles (NPs) p–n heterojunction as the photoelectrode, on ...
DOI:
10.1021/ac403691a

75. Acetylcholinesterase Inhibition, Antioxidant, Antiinflammatory, Antimicrobial and Phytochemical Properties of Huernia hystrix

Author:
Stephen O. Amoo; Jeffrey F. Finnie; Johannes Van Staden
Source:
Phytotherapy research 2012 v.26 no.5 pp. 639-645
ISSN:
0951-418X
Subject:
antimicrobial properties; traditional medicine; anti-inflammatory activity; acetylcholinesterase; butylated hydroxytoluene; stems; phenols; Apocynaceae; plant extracts; iridoids; flavonoids; antioxidant activity; enzyme inhibition; roots; antioxidants; South Africa; Ethiopia
Abstract:
... Huernia species are typical famine‐food plants consumed in southern Ethiopia. H. hystrix is a heavily exploited ethnomedicinal succulent plant traded in traditional medicine systems especially on South Africa's eastern seaboard. This study investigated the acetylcholinesterase (AChE) inhibition, antioxidant, antiinflammatory and antimicrobial properties of extracts obtained from the stems and root ...
DOI:
10.1002/ptr.3614
PubMed:
22517510

76. Acetylcholinesterase Inhibitory Activity and Chemical Composition of Commercial Essential Oils

Author:
Dohi, Satomi; Terasaki, Masanori; Makino, Masakazu
Source:
Journal of agricultural and food chemistry 2009 v.57 no.10 pp. 4313-4318
ISSN:
0021-8561
Subject:
inhibitory concentration 50; chemical composition; interspecific variation; acetylcholinesterase; gas chromatography; Ocimum tenuiflorum; Lavandula angustifolia; essential oils; enzyme inhibition; enzyme inhibitors; Inula; mass spectrometry; Artemisia dracunculus; essential oil crops; eugenol
DOI:
10.1021/jf804013j
PubMed:
19358605

77. Acetylcholinesterase Toxicity of Malathion and Its Metabolites

Author:
W. W. Walker; B. J. Stojanovic
Source:
Journal of environmental quality 1973 v.2 no.4 pp. 474-475
ISSN:
0047-2425
Subject:
Arthrobacter; acetylcholinesterase; cattle; dicarboxylic acids; enzyme inhibition; laboratory experimentation; malathion; metabolites; potassium; toxicity
Abstract:
... Malathion, under in vitro laboratory conditions, is readily degraded by an Arthrobacter species to four metabolites—malathion half-ester, malathion dicarboxylic acid, potassium dimethyl phosphorothioate, and potassium dimethyl phosphorodithioate. However, it is not clear whether or not this degradation represents a simultaneous detoxication of the malathion molecule. With this in mind, the relativ ...
DOI:
10.2134/jeq1973.00472425000200040015x

78. Acetylcholinesterase activity in Gammarus fossarum (Crustacea Amphipoda): Linking AChE inhibition and behavioural alteration

Author:
Xuereb, Benoît; Lefèvre, Estelle; Garric, Jeanne; Geffard, Olivier
Source:
Aquatic toxicology 2009 v.94 no.2 pp. 114-122
ISSN:
0166-445X
Subject:
water pollution; water quality; chlorpyrifos; methomyl; Gammarus; aquatic arthropods; acetylcholinesterase; enzyme inhibition; feeding behavior; locomotion; enzyme activity; toxicity testing
Abstract:
... Relations between whole-body acetylcholinesterase (AChE) inhibition and changes in feeding and locomotor behaviours were investigated in adult male Gammarus fossarum during short-term exposure (96h) to the organophosphorous pesticide chlorpyrifos (CPE) and the carbamate pesticide methomyl (MT). AChE activity was measured after 24, 48 and 96h of exposure. The feeding rate was assessed after 48 and ...
DOI:
10.1016/j.aquatox.2009.06.010
PubMed:
19608286

79. Acetylcholinesterase activity in fish species exposed to crude oil hydrocarbons: A review and new perspectives

Author:
Hugo F. Olivares-Rubio; Jesús Javier Espinosa-Aguirre
Source:
Chemosphere 2021 v.264 pp. 128401
ISSN:
0045-6535
Subject:
acetylcholinesterase; anthracenes; benzo(a)pyrene; biomarkers; chemical pollutants; environmental exposure; environmental monitoring; enzyme activity; enzyme inhibition; fish; gasoline; neurotoxicity; organophosphorus pesticides; petroleum; pyrene (hydrocarbon); water pollution
Abstract:
... Crude oil and its derivatives are primary energy resources for humans, and processes involving these materials could affect aquatic environments. Acetyl cholinesterase (AChE) activity is a suitable biomarker for exposure to organophosphate pesticides. Under controlled conditions, fish exposed to polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene, pyrene and anthracene, showed inhibiti ...
DOI:
10.1016/j.chemosphere.2020.128401

80. Acetylcholinesterase activity of alkaloids from the leaves of Waltheria brachypetala

Author:
Lima, Murilo M.C.; Lopez, Jorge A.; David, Jorge M.; Silva, Eliezer P.; Giulietti, Ana M.; Queiroz, Luciano P. de; David, Juceni P.
Source:
Planta medica 2009 v.75 no.4 pp. 335-337
ISSN:
0032-0943
Subject:
Malvaceae; medicinal plants; phytochemicals; quinolizidine alkaloids; medicinal properties; acetylcholinesterase; enzyme activity; enzyme inhibition; chemical structure; spectral analysis; traditional medicine; erythrocytes; cultured cells; human health; Brazil
DOI:
10.1055/s-0028-1112215
PubMed:
19101888

81. Acetylcholinesterase and Cytotoxic Activity of Chemical Constituents of Clutia lanceolata Leaves and its Molecular Docking Study

Author:
Mehtab Parveen; Faheem Ahmad; Ali Mohammed Malla; Shaista Azaz; Mahboob Alam; Omer A. Basudan; Manuela Ramos Silva; Pedro S. Pereira Silva
Source:
Nat. Prod. Bioprospect. 2016 v.6 no.6 pp. 267-278
ISSN:
2192-2195
Subject:
Artemia; Euphorbiaceae; Fourier transform infrared spectroscopy; X-radiation; acetylcholinesterase; active sites; chemical composition; chemical constituents of plants; cytotoxicity; density functional theory; enzyme inhibition; leaves; molecular models; mortality; nuclear magnetic resonance spectroscopy; spectral analysis
Abstract:
... Phytochemical investigations of the ethanolic extract of leaves of Clutia lanceolata (Family: Euphorbiaceae) resulted in the isolation of four compounds viz. 3,4-dihydroxy-2-methylbenzoic acid (1), 2,2′-dihydroxy-1,1′-binaphthyl (2), 1,3,8-trihydroxy-6-methylanthracene-9,10-dione (3) and 5-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,4,6-trien-3-one (4). Although all the isolated compounds we ...
DOI:
10.1007/s13659-016-0110-x
PubMed:
27757926
PubMed Central:
PMC5136371

82. Acetylcholinesterase as a target of halogenated marine natural products from Laurencia dendroidea

Author:
Karina Godarth Gonçalves; Leandro Louback da Silva; Angelica Ribeiro Soares; Nelilma Correia Romeiro
Source:
Algal research 2020 v.52 pp. 102130
ISSN:
2211-9264
Subject:
Alzheimer disease; Laurencia; acetylcholinesterase; algae; assays; benzene; bromine; chlorine; cholinesterase inhibitors; computer simulation; databases; drugs; enzyme inhibition; enzyme kinetics; metabolites; research; sesquiterpenoids; skeleton; stereochemistry; tryptophan
Abstract:
... The use of chemogenomics techniques applied to a marine natural products database has pointed out halogenated sesquiterpenes present in red alga Laurencia dendroidea as potential acetylcholinesterase inhibitors. Therefore, in this work five metabolites were studied using molecular docking and enzymatic inhibition assays. According to the results the most promising metabolite in relation to acetylc ...
DOI:
10.1016/j.algal.2020.102130

83. Acetylcholinesterase enzyme inhibitory effects of Amaryllidaceae alkaloids

Author:
Elgorashi, E.E.; Stafford, G.I.; Van Staden, J.
Source:
Planta medica 2004 v.70 no.3 pp. 260-262
ISSN:
0032-0943
Subject:
acetylcholinesterase; phytochemicals; Cyrtanthus; plant extracts; human health; structure-activity relationships; Crinum; enzyme inhibition; Liliaceae; lycorine
DOI:
10.1055/s-2004-818919
PubMed:
15114506

84. Acetylcholinesterase in selected plant-parasitic nematodes: Inhibition, kinetic and comparative studies

Author:
Al-Rehiayani, S.M.
Source:
Pesticide biochemistry and physiology 2008 v.90 no.1 pp. 19-25
ISSN:
0048-3575
Subject:
Meloidogyne javanica; Heterodera avenae; Tylenchulus semipenetrans; plant parasitic nematodes; acetylcholinesterase; enzyme activity; organophosphorus compounds; carbamates; kinetics; in vitro studies; protein content; enzyme inhibition; Saudi Arabia
Abstract:
... The in vitro inhibition potency of some organophosphates (OPs) and carbamates (CAs) which are widely used to control plant-parasitic nematodes on acetylcholinesterase (AChE) of Meloidogyne javanica, Heterodera avenae and Tylenchulus semipenetrans, the major pathogens responsible for the damage of a wide range of crops in Al-Qassim region, Saudi Arabia was examined. AChE of H. avenae activity was 1 ...
DOI:
10.1016/j.pestbp.2007.07.004

85. Acetylcholinesterase inhibitor from Anthocleista vogelii leaf extracts

Author:
O.S. Ajayi; M.A. Aderogba; E.M. Obuotor; R.R.T. Majinda
Source:
Journal of ethnopharmacology 2019 v.231 pp. 503-506
ISSN:
0378-8741
Subject:
Alzheimer disease; Anthocleista; acetylcholinesterase; brain; butanol; enzyme inhibition; enzyme inhibitors; ethyl acetate; fractionation; hexane; inflammation; inhibitory concentration 50; leaf extracts; methanol; methylene chloride; nuclear magnetic resonance spectroscopy; oxidative stress; physostigmine; solvents; traditional medicine
Abstract:
... Anthocleista vogelii (Planch) is used in folk medicine in conditions related to inflammation and oxidative stress. This suggests that some of its constituents could possess ability to reduce oxidative damage to cells and ameliorate inflammatory conditions which are two prominent features in the brain of a person suffering from Alzheimer’s disease.The leaf extracts were investigated for their bioac ...
DOI:
10.1016/j.jep.2018.11.009

86. Acetylcholinesterase inhibitory active metabolites from the endophytic fungus Colletotrichum sp. YMF432

Author:
Zhe Li; Ni Ma; Pei-Ji Zhao
Source:
Natural product research 2019 v.33 no.12 pp. 1794-1797
ISSN:
1478-6427
Subject:
Chaetomium; Colletotrichum; Huperzia serrata; acetylcholinesterase; benzoquinones; beta-sitosterol; endophytes; enzyme inhibition; fermentation; fungi; inhibitory concentration 50; metabolites; spectral analysis
Abstract:
... An endophytic fungus, Chaetomium sp. YMF432, was isolated from Huperzia serrata (Thunb. ex Murray) Trev. and subjected to phytochemical investigation based on its special environment. From the extracts of fermentation solid of strain YMF 432, eight compounds including 1-O-methylemodin (1), 5-methoxy-2-methyl-3-tricosyl-1,4-benzoquinone (2), 4,8-dihydroxy-1-tetralone (3), (3β,5α,6α, 22E)-3-hydroxy- ...
DOI:
10.1080/14786419.2018.1434648

87. Acetylcholinesterase inhibitory activity and neuroprotection in vitro, molecular docking, and improved learning and memory functions of demethylcurcumin in scopolamine-induced amnesia ICR mice

Author:
Yuh-Hwa Liu; Chia-Jung Lee; Liang-Chieh Chen; Tai-Lin Lee; Ying-Ying Hsieh; Chuan-Hsiao Han; Chang-Hang Yang; Wei-Jan Huang; Wen-Chi Hou
Source:
Food & function 2020 v.11 no.3 pp. 2328-2338
ISSN:
2042-650X
Subject:
acetylcholinesterase; amnesia; animal experimentation; binding sites; brain; computer simulation; curcumin; enzyme activity; enzyme inhibition; functional foods; hydrogen bonding; macrophages; memory; mice; models; moieties; neuroprotective effect; peptides
Abstract:
... In this study, demethylcurcumin (DC), a minor constituent in curcuminoids, showed better anti-acetylcholinesterase (anti-AChE) activities, anti-amyloid β peptide aggregation, neuroprotective activities in 6-hydroxydopamine-treated SH-SY5Y cell models, and anti-nitric oxide production in lipopolysaccharide-treated RAW 264.7 macrophages than those of curcumin. Based on molecular docking analyses wit ...
DOI:
10.1039/c9fo02339a

88. Acetylcholinesterase inhibitory activity of a neurosteroidal alkaloid from the upside-down jellyfish Cassiopea andromeda venom

Author:
Gholamhossein Mohebbi; Iraj Nabipour; Amir Vazirizadeh; Hossein Vatanpour; Maryam Farrokhnia; Ammar Maryamabadi; Afshar Bargahi
Source:
Revista Brasileira de Farmacognosia 2018 v.28 no.5 pp. 568-574
ISSN:
0102-695X
Subject:
Alzheimer disease; Scyphozoa; acetylcholinesterase; alkaloids; amino acids; aquatic organisms; enzyme inhibition; fractionation; gas chromatography-mass spectrometry; gel chromatography; hydrophobicity; inhibitory concentration 50; nuclear magnetic resonance spectroscopy; reference standards; spectral analysis; venoms
Abstract:
... Natural compounds from marine organisms have been rarely studied for their acetylcholinesterase inhibitory activities. The aim of this study was to isolate novel compounds with antiAChE activity from the venom of upside-down jellyfish Cassiopea andromeda Forskål, 1775. The compounds of the fractionated venom on gel filtration chromatography were identified by analyzing gas chromatography–mass spec ...
DOI:
10.1016/j.bjp.2018.06.002

89. Acetylcholinesterase inhibitory activity of didehydrostemofoline, stemofoline alkaloids and extracts from Stemona collinsiae Craib roots

Author:
Sumet Kongkiatpaiboon; Piyanuch Rojsanga; Virote Pattarajinda; Wandee Gritsanapan
Source:
Pharmacognosy Journal 2013 v.5 pp. 56-59
ISSN:
0975-3575
Subject:
Stemona; acetylcholinesterase; alkaloids; bioassays; colorimetry; enzyme inhibition; hexane; high performance liquid chromatography; inhibitory concentration 50; insecticides; insects; methanol; methylene chloride; pharmacognosy; roots; toxicity
Abstract:
... Acetylcholinesterase (AChE) inhibition is one of insect toxicity mechanisms found in many plants. Stemona collinsiae Craib, a traditional insecticidal plant, has been of interest for its AChE inhibitory activity.Powdered roots of S. collinsiae were successively extracted with hexane, dichloromethane, and methanol. The contents of major active insecticidal components, didehydrostemofoline and stemo ...
DOI:
10.1016/j.phcgj.2013.02.004

90. Acetylcholinesterase inhibitory and antioxidant properties of Cyclotrichium niveum, Thymus praecox subsp. caucasicus var. caucasicus, Echinacea purpurea and E. pallida

Author:
Orhan, I.; Şenol, F.S.; Gülpinar, A.R.; Kartal, M.; Şekeroglu, N.; Deveci, M.; Kan, Y.; Şener, B.
Source:
Food and chemical toxicology 2009 v.47 no.6 pp. 1304-1310
ISSN:
0278-6915
Subject:
spectrophotometers; acetylcholinesterase; Thymus (Lamiaceae); Echinacea pallida; plant extracts; flowers; essential oils; free radical scavengers; enzyme inhibitors; indigenous species; Echinacea purpurea; antioxidant activity; enzyme inhibition; leaves; Turkey (country)
DOI:
10.1016/j.fct.2009.03.004
PubMed:
19285534

91. Acetylcholinesterase inhibitory-active components of Rhodiola rosea L

Author:
Wang, H.; Zhou, G.; Gao, X.; Wang, Y.; Yao, W.
Source:
Food chemistry 2007 v.105 no.1 pp. 24-27
ISSN:
0308-8146
Subject:
acetylcholinesterase; enzyme inhibition; Rhodiola rosea; rhizomes; plant extracts; hydroquinone; chemical constituents of plants; Alzheimer disease
Abstract:
... An activity-directed fractionation and purification process was used to identify the acetylcholinesterase inhibitory-active components of Rhodiola rosea L. (RR). Dried rhizome of RR was extracted with boiled ethanol. After removal of tannins, the extract was separated into chloroform, ethyl acetate, n-butanol and water fractions. Among these, chloroform and n-butanol fractions showed stronger acti ...
DOI:
10.1016/j.foodchem.2007.03.031

92. Acetylcholinesterase mutation in an insecticide-resistant population of the codling moth Cydia pomonella (L.)

Author:
Cassanelli, S.; Reyes, M.; Rault, M.; Manicardi, G.C.; Sauphanor, B.
Source:
Insect biochemistry and molecular biology 2006 v.36 no.8 pp. 642-653
ISSN:
0965-1748
Subject:
acetylcholinesterase; selection response; insecticides; complementary DNA; amino acid substitution; amino acid sequences; resistance mechanisms; nucleotide sequences; active sites; insecticide resistance; enzyme inhibition; Cydia pomonella
Abstract:
... Two strains of Cydia pomonella (L.) (Lepidoptera: Tortricidae) were selected in the lab by exposure to increasing concentrations of diflubenzuron (Rdfb strain) or azinphos-methyl (Raz strain). Insecticide bioassays showed that the adults of the Rdfb strain exhibited a 2.6-fold and a 7.7-fold resistance ratio to azinphos-methyl and carbaryl, respectively compared to a susceptible strain (S) whereas ...
DOI:
10.1016/j.ibmb.2006.05.007
PubMed:
16876707

93. Acetylcholinesterase of the sand fly, Phlebotomus papatasi (Scopoli): cDNA sequence, baculovirus expression, and biochemical properties

Author:
Kevin B Temeyer; Danett K Brake; Alexander P Tuckow; Andrew Y Li; Adalberto A Pérez deLeón
Source:
Parasites & vectors 2013 v.6 no.1 pp. 31
ISSN:
1756-3305
Subject:
Aedes aegypti; Baculoviridae; Culex tritaeniorhynchus; Leishmania; Lutzomyia longipalpis; Phlebotomus papatasi; acetylcholinesterase; amino acids; central nervous system; complementary DNA; cutaneous leishmaniasis; domestic animals; enzyme activity; enzyme inhibition; gene expression; genes; genotype; insects; malaoxon; messenger RNA; open reading frames; paraoxon; people; point mutation; recombinant fusion proteins; reverse transcriptase polymerase chain reaction; screening; substrate specificity; Africa; Asia
Abstract:
... BACKGROUND: Millions of people and domestic animals around the world are affected by leishmaniasis, a disease caused by various species of flagellated protozoans in the genus Leishmania that are transmitted by several sand fly species. Insecticides are widely used for sand fly population control to try to reduce or interrupt Leishmania transmission. Zoonotic cutaneous leishmaniasis caused by L. ma ...
DOI:
10.1186/1756-3305-6-31
PubMed:
23379291
PubMed Central:
PMC3598880

94. Acetylcholinesterase, butyrylcholinesterase, and tyrosinase inhibition studies and antioxidant activities of 33 Scutellaria L. taxa from Turkey

Author:
Şenol, F.S.; Orhan, I.; Yilmaz, G.; Çiçek, M.; Şener, B.
Source:
Food and chemical toxicology 2010 v.48 no.3 pp. 781-788
ISSN:
0278-6915
Subject:
acetylcholinesterase; Scutellaria; enzyme inhibitors; enzyme inhibition; antioxidant activity; flavonoids; free radical scavengers; plant extracts; phenolic compounds; medicinal plants; Turkey (country)
DOI:
10.1016/j.fct.2009.12.004
PubMed:
20026160

95. Acetylcholinesterases from Leaf-Cutting ant Atta sexdens: Purification, Characterization, and Capillary Reactors for On-Flow Assays

Author:
Adriana M. Dos Santos; Ariele C. Moreira; Bianca Rebelo Lopes; Mariana F. Fracola; Fernando G. de Almeida; Odair C. Bueno; Quezia B. Cass; Dulce Helena F. Souza
Source:
Enzyme research 2019 v.2019 pp. -
ISSN:
2090-0414
Subject:
Atta sexdens; acetates; acetylcholine; acetylcholinesterase; ammonium; batch systems; choline; colorimetry; death; enzyme inhibition; hydrolysis; ion exchange chromatography; leaf-cutting ants; neurotransmitters; pH; phylogeny; sulfates; tandem mass spectrometry
Abstract:
... Acetylcholinesterase (AChE) is responsible for catalyzing the hydrolysis of the neurotransmitter acetylcholine (ACh) leading to acetate and choline (Ch) release. The inhibition of AChE produces a generalized synaptic collapse that can lead to insect death. Herein we report for the first time the isolation of two AChEs from Atta sexdens which were purified by sulphate ammonium precipitation followe ...
DOI:
10.1155/2019/6139863
PubMed:
31354985
PubMed Central:
PMC6633970

96. Acid ceramidase inhibition sensitizes human colon cancer cells to oxaliplatin through downregulation of transglutaminase 2 and β1 integrin/FAK−mediated signalling

Author:
Marko Klobučar; Petra Grbčić; Sandra Kraljević Pavelić; Nives Jonjić; Sarah Visentin; Mirela Sedić
Source:
Biochemical and biophysical research communications 2018 v.503 no.2 pp. 843-848
ISSN:
0006-291X
Subject:
CD29 antigen; adenocarcinoma; adenoma; apoptosis; colorectal neoplasms; drug therapy; enzyme inhibition; human cell lines; humans; mucosa; neoplasm cells; patients; protein content; protein-glutamine gamma-glutamyltransferase; resistance mechanisms; transcription factor NF-kappa B
Abstract:
... Acid ceramidase (ASAH1) has been implicated in the progression and chemoresistance in different cancers. Its role in colon cancer biology and response to standard chemotherapy has been poorly addressed so far. Here, we have investigated ASAH1 expression at the protein level in human colon cancer cell lines and tissues from colon cancer patients, and have examined in vitro the possible link between ...
DOI:
10.1016/j.bbrc.2018.06.085

97. Acid induced reduction of the glycaemic response to starch-rich foods: the salivary α-amylase inhibition hypothesis

Author:
Daniela Freitas; Steven Le Feunteun
Source:
Food & function 2018 v.9 no.10 pp. 5096-5102
ISSN:
2042-650X
Subject:
acidification; alpha-amylase; beverages; blood glucose; diet; enzyme inhibition; gluten-free foods; glycemic effect; hydrolysis; lemon juice; oligosaccharides; pH; pasta; starch; wheat
Abstract:
... Numerous studies have reported that the glycaemic response to starch-rich meals can be reduced by 20–50% with acidic drinks or foods. A number of candidate explanations have been put forward, but this phenomenon still remains vaguely understood. This study intends to demonstrate the remarkable effect of acid inhibition of salivary α-amylase during oro-gastric hydrolysis of starch, shedding light o ...
DOI:
10.1039/c8fo01489b

98. Aclonifen targets solanesyl diphosphate synthase, representing a novel mode of action for herbicides

Author:
Sabine Kahlau; Florian Schröder; Jörg Freigang; Bernd Laber; Gudrun Lange; Daniel Passon; Sabrina Kleeßen; Marc Lohse; Arno Schulz; Pascal von Koskull‐Döring; Sebastian Klie; Sascha Gille
Source:
Pest management science 2020 v.76 no.10 pp. 3377-3388
ISSN:
1526-498X
Subject:
4-hydroxyphenylpyruvate dioxygenase; Arabidopsis thaliana; Chlamydomonas reinhardtii; acifluorfen; bifenox; biosynthesis; bleaching; carotenoids; diphenyl ethers; enzyme inhibition; enzyme inhibitors; enzyme substrates; herbicide resistance; leaves; mechanism of action; necrosis; norflurazon; oxadiazon; phenotype; protoporphyrinogen oxidase; transferases
Abstract:
... BACKGROUND: Aclonifen is a unique diphenyl ether herbicide. Despite its structural similarities to known inhibitors of the protoporphyrinogen oxidase (e.g. acifluorfen, bifenox or oxadiazon), which result in leaf necrosis, aclonifen causes a different phenotype that is described as bleaching. This also is reflected by the Herbicide Resistance Action Committee (HRAC) classification that categorizes ...
DOI:
10.1002/ps.5781

99. Acrolein detection based on alcohol dehydrogenase inhibition

Author:
Ovidiu I. Covaci; Bogdan Bucur; Gabriel L. Radu
Source:
International journal of environmental analytical chemistry 2013 v.93 no.3 pp. 325-334
ISSN:
1029-0397
Subject:
acrolein; alcohol dehydrogenase; aldehyde dehydrogenase; cysteine; enzyme inhibition; glutamate dehydrogenase; glutathione; spectrophotometers
Abstract:
... This paper presents the effect of acrolein on three dehydrogenases and proposes a fast spectrometric method for acrolein analysis. We have found that alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (AlDH) are inhibited by low acrolein concentrations (0.2 mM) while inhibition of glutamate dehydrogenase (GDH) is not observed even at higher acrolein concentrations (1 mM). Acrolein is a suicide ...
DOI:
10.1080/03067319.2011.581374

100. Action mechanism of a novel herbicide, pyroxasulfone

Author:
Tanetani, Yoshitaka; Kaku, Koichiro; Kawai, Kiyoshi; Fujioka, Tomonori; Shimizu, Tsutomu
Source:
Pesticide biochemistry and physiology 2009 v.95 no.1 pp. 47-55
ISSN:
0048-3575
Subject:
pyroxasulfone; mechanism of action; long chain fatty acids; biosynthesis; rice; millets; Lolium; wheat; corn; enzyme inhibition; amino acids; sequence analysis; polymerase chain reaction; plant growth
Abstract:
... The mechanism of action of pyroxasulfone was studied by examination of the inhibitory effects of this herbicide on the biosynthesis of very-long-chain fatty acids (VLCFAs) both in vivo and in vitro. Pyroxasulfone treatment drastically reduced the biosynthesis of VLCFAs and caused a buildup of fatty acid precursors in cultured rice cells. Pyroxasulfone specifically inhibited the elongation steps fr ...
DOI:
10.1016/j.pestbp.2009.06.003