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enzyme-linked immunosorbent assay
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corn
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- Author:
- Munusamy Mohankumar; Ayyathurai Vijayasamundeeswari; Muthusamy Karthikeyan; Subramanian Mathiyazhagan; Vaikuntavasan Paranidharan; Rethinasamy Velazhahan
- Source:
- Journal of plant protection research 2010 v.50 no.4 pp. 446-451
- ISSN:
- 1899-007X
- Subject:
- Aspergillus flavus; DNA primers; aflatoxin B1; agroecological zones; arithmetics; cluster analysis; corn; digestion; enzyme-linked immunosorbent assay; genetic variation; internal transcribed spacers; polymerase chain reaction; restriction endonucleases; restriction fragment length polymorphism; ribosomal DNA; India
- Abstract:
- ... A total of seventeen isolates of Aspergillus flavus from maize were collected from different agro-ecological zones of Tamil Nadu, India. The isolates were tested for their ability to produce aflatoxin B₁ (AFB₁) in vitro by indirect competitive enzyme-linked immunosorbent assay (ELISA). The amount of AFBM₁ produced by the isolates of A. flavus ranged from 1.9 to 206.6 ng/ml. Among the various isola ...
- DOI:
- 10.2478/v10045-010-0075-4
- https://doi.org/10.2478/v10045-010-0075-4
- Author:
- Linga R. Gutha; Divakar Purushottam; Aruna Veeramachaneni; Sarita Tigulla; Vikas Kodappully; Chandana Enjala; Hitendrasinh Rajput; Jennifer Anderson; Bonnie Hong; Jean Schmidt; Shveta Bagga
- Source:
- Transgenic research 2018 v.27 no.4 pp. 331-342
- ISSN:
- 0962-8819
- Subject:
- actin; corn; crops; developmental stages; enzyme-linked immunosorbent assay; field experimentation; genotype; glyceraldehyde-3-phosphate dehydrogenase; growing season; hybrids; leaves; models; peptide elongation factors; plant tissues; safety assessment; transgenic plants; India
- Abstract:
- ... Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference o ...
- DOI:
- 10.1007/s11248-018-0077-y
- https://doi.org/10.1007/s11248-018-0077-y
- Author:
- Ayyathurai Vijayasamundeeswari; Munusamy Mohankumar; Muthusamy Karthikeyan; Selvaraj Vijayanandraj; Vaikuntavasen Paranidharan; Rethinasamy Velazhahan
- Source:
- Journal of plant protection research 2009 v.49 no.2 pp. 221-224
- ISSN:
- 1899-007X
- Subject:
- Aspergillus flavus; adverse effects; aflatoxin B1; agroecological zones; antiserum; corn; enzyme-linked immunosorbent assay; farmers; food contamination; foods; humans; livestock feeds; monitoring; poultry; poultry industry; seeds; serum albumin; supermarkets; surveys; India
- Abstract:
- ... Aflatoxins, a group of mycotoxins mainly produced by Aspergillus flavus and A. parasiticus, have adverse health effects on humans and livestock that ingest aflatoxin- contaminated food products and feeds. To secure the safety of food and feed, regular monitoring of aflatoxin levels is necessary. In order to understand the magnitude of aflatoxin contamination, a survey was conducted in different ag ...
- DOI:
- 10.2478/v10045-009-0033-1
- https://doi.org/10.2478/v10045-009-0033-1
- Author:
- S. Shweta; K. Kannan; S. Madhavan; R. Adhithya; V. Paranidharan; R. Velazhahan
- Source:
- Archiv für Phytopathologie und Pflanzenschutz 2013 v.46 no.18 pp. 2174-2181
- ISSN:
- 1477-2906
- Subject:
- Aspergillus flavus; DNA; aflatoxin B1; carcinogenicity; cluster analysis; corn; cotton; enzyme-linked immunosorbent assay; genetic variation; hosts; hot peppers; molecular weight; nontoxigenic strains; peanuts; random amplified polymorphic DNA technique; secondary metabolites; India
- Abstract:
- ... Aflatoxin contamination is a major problem in maize, groundnut, chillies, cotton and tree nuts. These aflatoxins are low molecular weight toxic and carcinogenic secondary metabolites produced by Aspergillus flavus , A. parasiticus and A. nomius . In the present study, a total of 11 isolates of A. flavus isolated from groundnut, maize and chilli collected from different locations of Tamil Nadu, Ind ...
- DOI:
- 10.1080/03235408.2013.787701
- https://doi.org/10.1080/03235408.2013.787701