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- B. Loye Eberhart; Annette S. Wilson; Stephen J.D. O'Keefe; Matsepo C. Ramaboli; Lucky T. Nesengani
- Analytical biochemistry 2021 v.612 pp. 114016
- accuracy; butyric acid; chemical species; detection limit; ethyl ether; extraction; feces; formic acid; hexanoic acid; humans; isobutyric acid; measurement; propionic acid; research; sampling; sodium sulfate; valeric acid
- ... A one-vial extraction method for the quantitation of short-chain fatty acids (SCFAs) in human stool was developed. Samples were extracted with an acidified aqueous internal standard solution, sodium sulfate, and diethyl ether, followed by analysis with GC-FID. Accuracy, in terms of relative recovery, was typically between 90 and 110% for most analytes; without internal standard, the accuracy was a ...
- Serena Scortichini; Maria Chiara Boarelli; Stefania Silvi; Dennis Fiorini
- Journal of chromatography 2020 v.1143 pp. 121972
- acetic acid; acidification; chemical species; derivatization; ethyl ether; feces; fermentation; flame ionization; hexanoic acid; humans; intestinal microorganisms; metabolites; mice; polyethylene glycol; propionic acid; quantitative analysis; rats
- ... Short-chain fatty acids (SCFAs) are gut microbiota metabolites recognized for their beneficial effects on the host organism. In this study, a simple and rapid sample preparation method combined to SCFAs analysis by direct injection and gas chromatography coupled with flame ionization detection (GC-FID), for the determination and quantification of eight SCFAs (acetic, propionic, i-butyric, butyric, ...