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- Author:
- T. Hailay; M. Hoelker; S. Gebremedhn; F. Rings; M. M. Saeed-Zidane; M. Poirier; D. Salilew-Wondim; C. Dauben; E. Tholen; C. Neuhoff; K. Schellander; D. Tesfaye
- Source:
- Reproduction, fertility, and development 2019 v.31 no.1 pp. 183-184
- ISSN:
- 1031-3613
- Subject:
- 3-hydroxybutyric acid; Holstein; blood; body weight; cell communication; cell cycle; dairy cows; dry matter intake; early lactation; electron microscopy; energy balance; exosomes; female fertility; follicular development; follicular fluid; free fatty acids; gene expression regulation; heifers; high-throughput nucleotide sequencing; microRNA; oocytes; ovulation; prediction; proteins; ultracentrifugation; uterine neoplasms
- Abstract:
- ... Most high-milking cows enter a state of negative energy balance during the early lactation period. This phenomenon disturbs the metabolic status of the follicular fluid microenvironment, resulting in delayed ovulation. Cell-to-cell communication between the oocyte and the surrounding cells is crucial during folliculogenesis. Exosomes, evolutionarily conserved cargo molecules (30-150nm in diameter) ...
- DOI:
- 10.1071/RDv31n1Ab115
- https://doi.org/10.1071/RDv31n1Ab115
- Author:
- S. Uzbekova; C. Alminana-Brines; V. Labas; L. Combes-Soia; I. I. Kireev; A.-P. Teixeira-Gomes; R. E. Uzbekov; G. N. Singina
- Source:
- Reproduction, fertility, and development 2020 v.32 no.2 pp. 188
- ISSN:
- 1031-3613
- Subject:
- NAD (coenzyme); RNA-binding proteins; antioxidant activity; carbon; cattle; cell adhesion; cell communication; cell cycle; cell proliferation; cholesterol; computer software; embryo (animal); endocytosis; endoplasmic reticulum; exosomes; fatty acids; follicular fluid; gene ontology; granulosa cells; liquid chromatography; messenger RNA; metabolism; mitochondria; non-coding RNA; oocytes; peptides; polyacrylamide gel electrophoresis; protein content; protein folding; protein isoforms; proteolysis; proteome; ribosomal RNA; ribosomal proteins; sodium dodecyl sulfate; tandem mass spectrometry; translation (genetics); transmission electron microscopy; trypsin
- Abstract:
- ... Extracellular vesicles (EVs) are released from cells and are present in most bodily fluids. Small EVs, 30-150nm in diameter, known as exosomes, transport regulatory noncoding RNA, lipids, and proteins, and thus participate in cell-to-cell communications and signalling. Exosomes from bovine ovarian follicular fluid (FF) can stimulate proliferation of granulosa cells (GCs), cellular uptake, and cumu ...
- DOI:
- 10.1071/RDv32n2Ab122
- https://doi.org/10.1071/RDv32n2Ab122
- Author:
- G. Santos; M. P. Bottino; A. P. C. Santos; R. E. Orlandi; L. M. S. Simões; J. C. Souza; M. B. D. Ferreira; J. C. Silveira; A. C. F. C. M. Ávila; A. Bridi; J. N. S. Sales
- Source:
- Reproduction, fertility, and development 2019 v.31 no.1 pp. 188-189
- ISSN:
- 1031-3613
- Subject:
- Bos; apoptosis; cloprostenol; dairy cows; equine chorionic gonadotropin; estradiol; exosomes; female fertility; follicular fluid; gene expression; granulosa cells; intravaginal devices; mastitis; messenger RNA; oocytes; ovulation; particle size; phosphatidylinositol 3-kinase; progesterone; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; somatic cell count; statistical analysis
- Abstract:
- ... The objective of this study was to evaluate the effect of mastitis diagnosed by somatic cell count (SCC) on follicular growth, ovulation, oocytes and cumulus cells quality and the concentration and size of exosomes in follicular fluid of dairy cows. In the study, crossbred cows (Bos taurus-Holstein×Bos indicus-Gir) were classified for analysis as control (SCC <200.000 cells mL−1) and mastitis (SCC ...
- DOI:
- 10.1071/RDv31n1Ab126
- https://doi.org/10.1071/RDv31n1Ab126
- Author:
- S. C. Loux; K. E. Scoggin; J. E. Bruemmer; I. F. Canisso; M. H. Troedsson; E. L. Squires; B. A. Ball
- Source:
- Reproduction, fertility, and development 2016 v.28 no.2 pp. 207
- ISSN:
- 1031-3613
- Subject:
- biomarkers; blood; chorion; diestrus; endometrium; exosomes; gene expression regulation; half life; mares; microRNA; microarray technology; non-coding RNA; population; pregnancy; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; screening
- Abstract:
- ... MicroRNAs (miRNAs) are small, non-coding RNAs that are differentially expressed throughout the body, including the endometrium and chorion during gestation. Although they are produced by individual tissues, many of these miRNAs are excreted into circulation, either within exosomes or attached to protein carrier molecules. Circulating miRNAs are surprisingly stable, with an average half-life of 5 d ...
- DOI:
- 10.1071/RDv28n2Ab154
- https://doi.org/10.1071/RDv28n2Ab154
- Author:
- M. O. Taqi; S. Gebremedhn; D. Salilew-Wondim; F. Rings; C. Neuhoff; E. Tholen; E. Held-Hoelker; M. Hoelker; K. Schellander; D. Tesfaye
- Source:
- Reproduction, fertility, and development 2019 v.31 no.1 pp. 213-214
- ISSN:
- 1031-3613
- Subject:
- amino acids; analysis of variance; blastocyst; cattle; culture media; embryo culture; embryogenesis; exosomes; females; fluorescent antibody technique; gene expression; homeostasis; males; messenger RNA; oxidative stress; oxygen; phenotype; protein synthesis; reactive oxygen species; reverse transcriptase polymerase chain reaction; semen; stress response; transcription factors; zygote
- Abstract:
- ... Pre-implantation embryo development is a critical stage, in which several development and stress-response related transcription factors (TF) are involved. Exposing embryos to environmental insults alter some of these stress response-related TF. However, their expression pattern in male and female embryos and their release via exosomes is still unclear. Here, we aimed to investigate the effect of c ...
- DOI:
- 10.1071/RDv31n1Ab178
- https://doi.org/10.1071/RDv31n1Ab178
- Author:
- Fumihiro Shiromoto; Hussein H. Aly; Haruka Kudo; Koichi Watashi; Asako Murayama; Noriyuki Watanabe; Xin Zheng; Takanobu Kato; Kazuaki Chayama; Masamichi Muramatsu; Takaji Wakita
- Source:
- Biochemical and biophysical research communications 2018 v.503 no.3 pp. 1854-1860
- ISSN:
- 0006-291X
- Subject:
- Hepatitis B virus; exosomes; hepatoma; inflammation; interleukin-1beta; messenger RNA; transcription factors
- Abstract:
- ... Hepatitis B virus (HBV) -x protein is a transcriptional regulator required for the HBV life cycle. HBx also induces complications in the host such as hepatocellular carcinoma. We previously showed that HBx mRNA is degraded by the Ski2/RNA exosome complex. In the present study, we report the regulation of this system through the control of Ski2 expression. We identified interleukin (IL) -1β as an i ...
- DOI:
- 10.1016/j.bbrc.2018.07.126
- https://doi.org/10.1016/j.bbrc.2018.07.126
- Author:
- N. Gupta; K. Polkoff; L. Qiao; K. Cheng; J. Piedrahita
- Source:
- Reproduction, fertility, and development 2019 v.31 no.1 pp. 225
- ISSN:
- 1031-3613
- Subject:
- DNA; Dependoparvovirus; RNA; Western blotting; computer software; dyes; exosomes; extrusion; fibroblasts; filtration; flow cytometry; gene editing; green fluorescent protein; ice; immune response; immune system; lipids; lipophilicity; molecular weight; particle size; peptidase K; polymerase chain reaction; saponins; sonication; staining; swine; therapeutics; transgenes; transmission electron microscopy
- Abstract:
- ... CRISPR/Cas systems present a powerful gene-editing tool with the potential for widespread therapeutic use; however, current methods of in vivo delivery such as adeno-associated viruses (AAV) may stimulate an immune response, creating the need for an alternative for delivery of CRISPR/Cas9. Exosomes are small vesicles that are released by cells and serve as a delivery system for RNA, proteins, and ...
- DOI:
- 10.1071/RDv31n1Ab200
- https://doi.org/10.1071/RDv31n1Ab200
- Author:
- A. Carothers; R. Dahal; N. Songsasen; M. Ferraz
- Source:
- Reproduction, fertility, and development 2019 v.31 no.1 pp. 229
- ISSN:
- 1031-3613
- Subject:
- Felidae; acrosome; boron; castration; cats; computer software; dyes; epididymis; epinephrine; exosomes; fluorescence microscopes; heparin; in vitro fertilization; models; oocytes; oviducts; penicillamine; penicillins; polyspermy; pregnancy; semen; spaying; sperm capacitation; sperm motility; streptomycin; t-test; transmission electron microscopy; zygote
- Abstract:
- ... Exosomes play important roles in reproduction, for example, facilitating fetal-maternal interaction and establishment of pregnancy. However, little is known about how oviductal exosomes affect sperm function. We investigated the effects of oviductal exosomes on sperm capacitation and fertilizing ability in domestic cats as a model for endangered felids. Oviducts were collected from cats (1-5 years ...
- DOI:
- 10.1071/RDv31n1Ab207
- https://doi.org/10.1071/RDv31n1Ab207
- Author:
- S. Kamen; J. Nagashima; N. Songsasen; M. Ferraz
- Source:
- Reproduction, fertility, and development 2020 v.32 no.2 pp. 242-243
- ISSN:
- 1031-3613
- Subject:
- Canis rufus; acrosome; adults; apoptosis; assisted reproductive technologies; biomarkers; carbon dioxide; cold tolerance; cryopreservation; culture media; dogs; endangered species; energy metabolism; estrous cycle; exosomes; freeze-thaw cycles; gene expression regulation; genetic variation; glycerol; males; mitochondria; oviducts; proteins; proteomics; spaying; sperm motility; t-test; thawing; wolves
- Abstract:
- ... Development of assisted reproductive technologies for the critically endangered red wolf (Canis rufus) is crucial to the maintenance of genetic diversity to support species recovery. Towards this goal, a cryopreservation protocol has previously been developed for red wolf sperm; however, the ability of the gametes to undergo capacitation has not been assessed in this species. Previously, we have s ...
- DOI:
- 10.1071/RDv32n2Ab229
- https://doi.org/10.1071/RDv32n2Ab229
- Author:
- Feng Zhu; Dan Li; Qinfeng Ding; Chong Lei; Lizhu Ren; Xianguang Ding; Xuecheng Sun
- Source:
- Biosensors & bioelectronics 2020 v.147 pp. 111787
- ISSN:
- 0956-5663
- Subject:
- biopsy; biosensors; cell communication; detection limit; engineering; exosomes; iron oxides; magnetism; molybdenum disulfide; nanocomposites; nanoparticles; neoplasms; oligonucleotides; prognosis; screening
- Abstract:
- ... Exosome released from cells plays an important role in intercellular communication and show great clinical potential in early cancer screening and prognosis. Herein, an ultrasensitive Giant Magnetoresistance (GMR) biosensor was developed for exosome detection, which was based on 2D MoS₂–Fe₃O₄ nanostructures (MOFE) as magnetic responsive probes for signal amplification. The MOFE can be readily synt ...
- DOI:
- 10.1016/j.bios.2019.111787
- https://doi.org/10.1016/j.bios.2019.111787
- Author:
- J. Cabezas; D. Rojas; B. Melo-Baez; M. Gutierrez; F. Castro; L. Rodriguez-Alvarez
- Source:
- Reproduction, fertility, and development 2020 v.32 no.2 pp. 151
- ISSN:
- 1031-3613
- Subject:
- blastocyst; carbon dioxide; cattle; centrifugation; culture media; embryogenesis; endometrium; epithelium; exosomes; filtration; flow cytometry; gene expression; nanoparticles; oocytes; oviducts; oxygen; progesterone; protein content; proteins; slaughterhouses; stromal cells; transmission electron microscopy; zygote
- Abstract:
- ... The success of development of invitro embryo production needs to mimic culture conditions in the maternal environment. Recently, it has been seen that extracellular vesicles (EVs) secreted by oviducal or endometrial cells may improve development and quality of embryos produced invitro. Extracellular vesicles are a mechanism of cellular communication; they carry molecules that are delivered into th ...
- DOI:
- 10.1071/RDv32n2Ab52
- https://doi.org/10.1071/RDv32n2Ab52
- Author:
- B. Melo-Baez; E. Mellisho; L. Rodriguez-Alvarez
- Source:
- Reproduction, fertility, and development 2019 v.31 no.1 pp. 156
- ISSN:
- 1031-3613
- Subject:
- blastocyst; carbon dioxide; cattle; cell communication; cell proliferation; confocal microscopy; culture media; dyes; early development; embryo culture; embryogenesis; exosomes; fetal bovine serum; fluorescence; image analysis; in vitro fertilization; oocytes; oxygen; protein sources; ultrafiltration; washing; zygote
- Abstract:
- ... Extracellular vesicles (EV) are currently considered a mechanism of cell communication. These are secreted from different cell types, including embryos, to serve as mediators of short and long distance signals. EV can be identified in vivo in different biological fluids, as well as in vitro embryo culture medium. Usually, media used for embryo in vitro culture are supplemented with serum or other ...
- DOI:
- 10.1071/RDv31n1Ab61
- https://doi.org/10.1071/RDv31n1Ab61
- Author:
- C. Almiñana; E. Corbin; G. Harichaux; V. Labas; G. Tsikis; C. Soleilhavoup; K. Reynaud; X. Druart; P. Mermillod
- Source:
- Reproduction, fertility, and development 2016 v.28 no.2 pp. 168
- ISSN:
- 1031-3613
- Subject:
- actin; blastocyst; carbon dioxide; cell communication; communications technology; confocal microscopy; cows; estrous cycle; exosomes; fluorescent dyes; genes; hatching; light scattering; metabolism; oviducts; oxygen; pregnancy; proteomics; ultracentrifugation; uterus; zona pellucida
- Abstract:
- ... Successful pregnancy requires an appropriate communication between the mother and the embryo(s). Recent studies indicate that exosomes, small (30–100nm) membrane-bound vesicles of endocytotic origin, could act as intercellular vehicles in this unique communication system in the uterus. However, little is known about the role of these vesicles in the oviduct. Our study aimed at (1) demonstrating th ...
- DOI:
- 10.1071/RDv28n2Ab77
- https://doi.org/10.1071/RDv28n2Ab77
- Author:
- B. Melo-Baez; Y. S. Wong; J. Cabezas; C. J. Aguilera; F. O. Castro; L. Rodriguez-Alvarez
- Source:
- Reproduction, fertility, and development 2020 v.32 no.2 pp. 166
- ISSN:
- 1031-3613
- Subject:
- biomarkers; biosynthesis; blastocyst; cattle; computer software; culture media; exosomes; fatty acids; flow cytometry; gap junctions; gene expression; gene expression regulation; genes; lysine; microRNA; nanoparticles; principal component analysis; signal transduction; stem cells; transmission electron microscopy; ultrafiltration; Canada; Chile
- Abstract:
- ... Extracellular vesicles (EVs), including exosomes and microvesicles, are secreted by different cell types and participate in cellular communication by carrying molecules as microRNAs (miRNAs) that can interfere with gene expression of target cells. Extracellular vesicles have become relevant as a mechanism of embryo-maternal communication. The aim of this study was to evaluate miRNA content in EVs ...
- DOI:
- 10.1071/RDv32n2Ab79
- https://doi.org/10.1071/RDv32n2Ab79
- Author:
- C. Aguilera; D. Veraguas; C. Henriquez; A. Velasquez; F. O. Castro; L. Rodriguez-Alvarez
- Source:
- Reproduction, fertility, and development 2020 v.32 no.2 pp. 166
- ISSN:
- 1031-3613
- Subject:
- DNA; chromosome aberrations; chromosomes; comparative genomic hybridization; culture media; embryo (animal); embryo culture; exosomes; humans; intracytoplasmic sperm injection; lipids; messenger RNA; microarray technology; nanoparticles; proteins; transmission electron microscopy; viability; Chile
- Abstract:
- ... Noninvasive methods are the clue to increase the efficiency of invitro-derived embryo selection without decreasing their competence. Embryos selection based on their morphology is the most used method but only 40% of selected embryos are able to implant and develop correctly. In humans, pre-implantation genetic diagnosis increases the efficiency of selection by excluding embryos with chromosomal a ...
- DOI:
- 10.1071/RDv32n2Ab80
- https://doi.org/10.1071/RDv32n2Ab80
- Author:
- Nobuhiro Hiraishi; Yo-ichi Ishida; Masami Nagahama
- Source:
- Biochemical and biophysical research communications 2015 v.467 pp. 534-540
- ISSN:
- 0006-291X
- Subject:
- RNA; RNA helicases; adenosinetriphosphatase; amino acid sequences; biogenesis; cell nucleolus; exosomes; protein subunits; proteomics; regulatory proteins; ribosomal proteins; ribosomes; sequence homology; yeasts
- Abstract:
- ... Nuclear VCP-like 2 (NVL2) is a chaperone-like nucleolar ATPase of the AAA (ATPase associated with diverse cellular activities) family, which exhibits a high level of amino acid sequence similarity with the cytosolic AAA-ATPase VCP/p97. These proteins generally act on macromolecular complexes to stimulate energy-dependent release of their constituents. We previously showed that NVL2 interacts with ...
- DOI:
- 10.1016/j.bbrc.2015.09.160
- https://doi.org/10.1016/j.bbrc.2015.09.160
- Author:
- Zhen Sun; Hang Zhao; Bing Liu; Yang Gao; Wen-Hao Tang; Zhi-Heng Liu; Zhuo-Jing Luo
- Source:
- Life sciences 2021 v.265 pp. 118778
- ISSN:
- 0024-3205
- Subject:
- apoptosis; cell communication; cell movement; enzyme-linked immunosorbent assay; exosomes; inflammation; interleukin-6; intervertebral disks; phenotype; quantitative polymerase chain reaction; scoliosis; transmission electron microscopy
- Abstract:
- ... The intervertebral disc is the largest avascular organ of the body. Vascularization of the disc has been typically regarded as a pathological feature of intervertebral disc degeneration (IDD). However, the underlying mechanism of vascularization in IDD is still unclear. The current study aimed to investigate the role of AF cell derived exosome (AF-exo) in the interaction with human umbilical vein ...
- DOI:
- 10.1016/j.lfs.2020.118778
- https://doi.org/10.1016/j.lfs.2020.118778
- Author:
- Ruixuan Wang; Xiaowei Zhang; Haibo Ding; Ying Qiao; Xiaoxu Han; Wenqing Geng; Gefei Guan; Hualu Cui; Bin Zhao; Yuntao Wu; Guoxin Liang; Hong Shang
- Source:
- FEBS letters 2018 v.592 no.2 pp. 284-294
- ISSN:
- 0014-5793
- Subject:
- Hepatitis B virus; Human gammaherpesvirus 8; Human immunodeficiency virus 1; antibodies; antiretroviral agents; cytidine deaminase; exosomes; genome; therapeutics; virus replication
- Abstract:
- ... Activation‐induced cytidine deaminase (AID), a member of the APOBEC family that induces antibody diversification, has been shown to inhibit the replication of hepatitis B virus, Kaposi's sarcoma‐associated herpesvirus, and retro‐transposons. However, whether AID can inhibit human immunodeficiency virus 1 (HIV‐1) replication remains unclear. Here, we report that AID impairs the synthesis of HIV‐1 c ...
- DOI:
- 10.1002/1873-3468.12954
- https://doi.org/10.1002/1873-3468.12954
- Author:
- Jia Fang; Wulong Liang
- Source:
- Biochemical and biophysical research communications 2021 v.572 pp. 125-130
- ISSN:
- 0006-291X
- Subject:
- exosomes; hepatocytes; liver; liver failure; liver transplant; mesenchymal stromal cells; quercetin; research; vitamin A
- Abstract:
- ... Acute liver injury is a short-term burst of liver cell damage, which has many causes and complex mechanisms. Despite the unique ability of the liver to heal itself, there is still no effective treatment except liver transplantation for chronic liver injury or even liver failure caused by acute liver injury. Stem cell-derived exosomes are ideal drug carriers due to their unique immunomodulatory eff ...
- DOI:
- 10.1016/j.bbrc.2021.07.059
- https://doi.org/10.1016/j.bbrc.2021.07.059
- Author:
- Shao-hua Luan; Yu-qing Yang; Man-ping Ye; Hui Liu; Qiu-fan Rao; Jin-ling Kong; Fan-rong Wu
- Source:
- International journal of biological macromolecules 2022 v.211 pp. 128-139
- ISSN:
- 0141-8130
- Subject:
- B-lymphocytes; exosomes; genes; information exchange; ion channels; liver cirrhosis; microRNA; transcriptome
- Abstract:
- ... Activation of hepatic stellate cells (HSCs) is a key cause of liver fibrosis. However, the mechanisms leading to the activation of HSCs are not fully understood. In the pathological process, acid-sensing ion channel 1a (ASIC1a) is widely involved in the development of inflammatory diseases, suggesting that ASIC1a may play an important role in liver fibrosis. We found that in an acidic environment, ...
- DOI:
- 10.1016/j.ijbiomac.2022.05.041
- https://doi.org/10.1016/j.ijbiomac.2022.05.041