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1. ASIC1a promotes hepatic stellate cell activation through the exosomal miR-301a-3p/BTG1 pathway

Author:
Shao-hua Luan; Yu-qing Yang; Man-ping Ye; Hui Liu; Qiu-fan Rao; Jin-ling Kong; Fan-rong Wu
Source:
International journal of biological macromolecules 2022 v.211 pp. 128-139
ISSN:
0141-8130
Subject:
B-lymphocytes; exosomes; genes; information exchange; ion channels; liver cirrhosis; microRNA; transcriptome
Abstract:
... Activation of hepatic stellate cells (HSCs) is a key cause of liver fibrosis. However, the mechanisms leading to the activation of HSCs are not fully understood. In the pathological process, acid-sensing ion channel 1a (ASIC1a) is widely involved in the development of inflammatory diseases, suggesting that ASIC1a may play an important role in liver fibrosis. We found that in an acidic environment, ...
DOI:
10.1016/j.ijbiomac.2022.05.041

2. Adipocytes‐Derived Exosomal microRNA‐1224 Inhibits M2 Macrophage Polarization in Obesity‐Induced Adipose Tissue Inflammation via MSI2‐Mediated Wnt/β‐Catenin Axis

Author:
Dongdong Zhang; Xiaoyan Yao; Yaqin Teng; Tiantian Zhao; Liangyan Lin; Yuanyuan Li; Hongxia Shang; Yongjun Jin; Qingsong Jin
Source:
Molecular nutrition & food research 2022 v.66 no.18 pp. e2100889
ISSN:
1613-4125
Subject:
RNA; adipocytes; coculture; epididymis; exosomes; flow cytometry; food research; high fat diet; immunohistochemistry; inflammation; macrophages; phenotype; white adipose tissue
Abstract:
... SCOPE: Phenotypic switch of macrophage polarization in adipose tissue has been associated with obesity‐induced adipose tissue inflammation (OATI). Therefore, this study aims to explore the possible mechanism of adipocytes‐derived exosomes (ADEs) carrying microRNA‐1224 (miR‐1224) in M2 macrophage polarization of OATI. METHODS AND RESULTS: miR‐1224‐knockout (miR‐1224‐KO) mice for this study, and iso ...
DOI:
10.1002/mnfr.202100889

3. Adipose mesenchymal stem cell–derived exosomal microRNAs ameliorate polycystic ovary syndrome by protecting against metabolic disturbances

Author:
Maosheng Cao; Yun Zhao; Tong Chen; Zijiao Zhao; Boqi Zhang; Chenfeng Yuan; Xin Wang; Lu Chen; Nan Wang; Chunjin Li; Xu Zhou
Source:
Biomaterials 2022 v.288 pp. 121739
ISSN:
0142-9612
Subject:
B-lymphocytes; animal models; biocompatible materials; cytokines; exosomes; genes; immunity; liver; metabolic diseases; metabolism; microRNA; non-coding RNA; phenotype; polycystic ovary syndrome; rats; reproduction
Abstract:
... Polycystic ovary syndrome (PCOS) is the most common endocrine and metabolic disorder in women of childbearing age. Adipose mesenchymal stem cells (AMSCs) secrete cytokines involved in the regulation of metabolism and immunity. However, it remains unclear whether exosomes secreted by AMSCs (AMSC-EXOs) can rescue the polycystic phenotype and metabolic dysfunction in PCOS ovaries. Here, we show that ...
DOI:
10.1016/j.biomaterials.2022.121739

4. Advanced approaches for improving bioavailability and controlled release of anthocyanins

Author:
Yixiao Shen; Ning Zhang; Jinlong Tian; Guang Xin; Ling Liu; Xiyun Sun; Bin Li
Source:
Journal of controlled release 2022 v.341 pp. 285-299
ISSN:
0168-3659
Subject:
anthocyanins; bioavailability; color; exosomes; humans; intestinal absorption; polysaccharides; protective effect; risk; solubility
Abstract:
... Anthocyanins are a group of phytochemicals responsible for the purple or red color of plants. Additionally, they are recognized to have health promoting functions including anti-cardiovascular, anti-thrombotic, anti-diabetic, antimicrobial, neuroprotective, and visual protective effect as well as anti-cancer activities. Thus, consumption of anthocyanin supplement or anthocyanin-rich foods has been ...
DOI:
10.1016/j.jconrel.2021.11.031

5. Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer

Author:
Lidija Filipović; Milica Spasojević; Radivoje Prodanović; Aleksandra Korać; Suzana Matijaševic; Goran Brajušković; Ario de Marco; Milica Popović
Source:
New biotechnology 2022 v.69 pp. 36-48
ISSN:
1871-6784
Subject:
biomarkers; biotechnology; cell culture; chromatography; composite polymers; culture media; exosomes; fractionation; humans; lipoproteins; porous media
Abstract:
... Correct elucidation of physiological and pathological processes mediated by extracellular vesicles (EV) is highly dependent on the reliability of the method used for their purification. Currently available chemical/physical protocols for sample fractionation are time-consuming, often scarcely reproducible and their yields are low. Immuno-capture based approaches could represent an effective purifi ...
DOI:
10.1016/j.nbt.2022.03.001

6. All-in-One Strategy for Downstream Molecular Profiling of Tumor-Derived Exosomes

Author:
Shurong Wang; Ying He; Jiayin Lu; Yuqing Wang; Xiaofeng Wu; Guoquan Yan; Xiaoni Fang; Baohong Liu
Source:
ACS applied materials & interfaces 2022 v.14 no.32 pp. 36341-36352
ISSN:
1944-8252
Subject:
biomarkers; cell culture; cost effectiveness; culture media; exosomes; hydrophilicity; nanopores; proteolysis; proteomics; stomach neoplasms
Abstract:
... In light of the significance of exosomes in cancer diagnosis and treatment, it is important to understand the components and functions of exosomes. Herein, an all-in-one strategy has been proposed for comprehensive characterization of exosomal proteins based on nanoporous TiO₂ clusters acting as both an extractor for exosome isolation and a nanoreactor for downstream molecular profiling. With the ...
DOI:
10.1021/acsami.2c07143

7. Anti-infection roles of miR-155-5p packaged in exosomes secreted by dendritic cells infected with Toxoplasma gondii

Author:
Dan Jiang; Shuizhen Wu; Liqing Xu; Guantai Xie; Dongliang Li; Hongjuan Peng
Source:
Parasites & vectors 2022 v.15 no.1 pp. 3
ISSN:
1756-3305
Subject:
Protozoa; Toxoplasma gondii; Western blotting; cell communication; exosomes; genes; interleukin-6; macrophages; mice; pregnancy; secretion; tachyzoites; toxoplasmosis; transmission electron microscopy; ultracentrifugation
Abstract:
... BACKGROUND: Toxoplasma gondii is a zoonotic intracellular protozoon that is estimated to infect about 30% of the world’s population, resulting in toxoplasmosis in immunocompromised patients and adverse outcomes in cases of primary infection during pregnancy. Exosomes are tubular vesicles secreted by cells, and function in intercellular communication. It has been reported that the exosomes secreted ...
DOI:
10.1186/s13071-021-05003-x

8. Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis

Author:
Ryosuke Koide; Nozomi Hirane; Daiki Kambe; Yasuhiro Yokoi; Michiru Otaki; Shin-Ichiro Nishimura
Source:
Biomaterials 2022 v.280 pp. 121314
ISSN:
0142-9612
Subject:
biocompatible materials; biogenesis; exosomes; fluorescence; glycosylation; metastasis; models; neoplasm cells; neoplasms
Abstract:
... Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions. This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model “fluorescent cancer glyconanosomes” displaying N-glycans ...
DOI:
10.1016/j.biomaterials.2021.121314

9. Apoptotic bodies for advanced drug delivery and therapy

Author:
Min Zhou; Yong-Jiang Li; Yu-Cheng Tang; Xin-Yan Hao; Wen-Jie Xu; Da-Xiong Xiang; Jun-Yong Wu
Source:
Journal of controlled release 2022 v.351 pp. 394-406
ISSN:
0168-3659
Subject:
apoptosis; biochemical compounds; biocompatibility; disease diagnosis; drugs; exosomes; immunotherapy
Abstract:
... Extracellular vesicles (EVs) have emerged as promising candidates for multiple biomedical applications. Major types of EVs include exosomes, microvesicles, and apoptotic bodies (ABs). ABs are conferred most properties from parent cells in the final stages of apoptosis. A wide variety of sources and stable morphological features are endowed to ABs by the rigorous apoptotic program. ABs accommodate ...
DOI:
10.1016/j.jconrel.2022.09.045

10. Aptamer-Initiated Catalytic Hairpin Assembly Fluorescence Assay for Universal, Sensitive Exosome Detection

Author:
Jiaqi Zhou; Qiuyuan Lin; Zhipeng Huang; Huiwen Xiong; Bin Yang; Hui Chen; Jilie Kong
Source:
Analytical chemistry 2022 v.94 no.15 pp. 5723-5728
ISSN:
1520-6882
Subject:
analytical chemistry; biomarkers; blood serum; calibration; cell communication; detection limit; exosomes; fluorescence
Abstract:
... Cancer-cell-derived exosomes are regarded as noninvasive biomarkers for early cancer diagnosis because of their critical roles in intercellular communication and molecular exchange. A robust aptamer-initiated catalytic hairpin assembly (AICHA) fluorescence assay is proposed for universal, sensitive detection of cancer-derived exosomes. The AICHA was verified with the specific detection of MCF-7 ce ...
DOI:
10.1021/acs.analchem.2c00231

11. Assessment of microRNA profiles in small extracellular vesicles isolated from bovine colostrum with different immunoglobulin G concentrations

Author:
T. Ma; W. Li; Y. Chen; E.R. Cobo; C. Windeyer; L. Gamsjäger; Q. Diao; Y. Tu; L.L. Guan
Source:
JDS communications 2022 pp. -
ISSN:
2666-9102
Subject:
axons; bioinformatics; cow colostrum; exosomes; focal adhesions; immunoglobulin G; microRNA; neonates; passive immunity; standard deviation; urine
Abstract:
... The consumption of bovine colostrum by newborn calves during the first days of life is essential to ensure the transfer of passive immunity. In addition to critical IgG, colostrum also contains non-IgG biomolecules, including microRNA (miRNA). The present study investigated the profiles of miRNA in small extracellular vesicles (sEV) isolated from bovine colostrum with high (256.5 ± 5.7 mg/mL, mean ...
DOI:
10.3168/jdsc.2022-0225

12. Autophagy induced by Schwann cell-derived exosomes promotes recovery after spinal cord injury in rats

Author:
Dayu Pan; Shibo Zhu; Weixin Zhang; Zhijian Wei; Fuhan Yang; Zhenglong Guo; Guangzhi Ning; Shiqing Feng
Source:
Biotechnology letters 2022 v.44 no.1 pp. 129-142
ISSN:
0141-5492
Subject:
acetylation; animal injuries; apoptosis; autophagy; biotechnology; exosomes; microtubules; polymerization
Abstract:
... Spinal cord injury (SCI) is catastrophic to humans and society. However, there is currently no effective treatment for SCI. Autophagy is known to serve critical roles in both the physiological and pathological processes of the body, but its facilitatory and/or deleterious effects in SCI are yet to be completely elucidated. This study aimed to use primary Schwann cell-derived exosomes (SCDEs) to tr ...
DOI:
10.1007/s10529-021-03198-8

13. BMAL1 induces colorectal cancer metastasis by stimulating exosome secretion

Author:
Pengjuan Dong; Yaping Wang; Yutong Liu; Chunting Zhu; Jiaxin Lin; Ruizhe Qian; Luchun Hua; Chao Lu
Source:
Molecular biology reports 2022 v.49 no.1 pp. 373-384
ISSN:
0301-4851
Subject:
Western blotting; centrifugation; circadian clocks; circadian rhythm; colorectal neoplasms; exosomes; humans; luciferase; metastasis; molecular biology; secretion; transcription (genetics); transmission electron microscopy
Abstract:
... BACKGROUND: To adapt to daily changes in the external environment, organisms have developed circadian rhythm systems with a period of approximately 24 h. Many studies have reported that both circadian rhythms and exosomes play important roles in the development and metastasis of tumors. However, whether circadian clock genes can affect the progression of tumors by regulating exosomes remains uncle ...
DOI:
10.1007/s11033-021-06883-z

14. BMI1 promotes cholangiocarcinoma progression and correlates with antitumor immunity in an exosome-dependent manner

Author:
Zengli Liu; Chunxiao Hu; Lijie Zheng; Jialiang Liu; Kangshuai Li; Xingyong Li; Yue Wang; Wentao Mu; Tianli Chen; Anda Shi; Bo Qiu; Xin Zhang; Zongli Zhang; Yunfei Xu
Source:
Cellular and molecular life sciences 2022 v.79 no.9 pp. 469
ISSN:
1420-682X
Subject:
autocrine signaling; bile ducts; biomarkers; carcinogenesis; chemokines; early diagnosis; epithelium; exosomes; humans; immunity; metastasis; neoplasm progression; prognosis; ubiquitination
Abstract:
... BACKGROUND: Cholangiocarcinoma (CCA) is a class of malignant tumors originating from bile duct epithelial cells. Due to difficult early diagnosis and limited treatment, the prognosis of CCA is extremely poor. BMI1 is dysregulated in many human malignancies. However, the prognostic significance and oncogenic role of BMI1 in cholangiocarcinoma (CCA) are not well elucidated. METHODS: In the present s ...
DOI:
10.1007/s00018-022-04500-1

15. The BMSC-derived exosomal lncRNA Mir9-3hg suppresses cardiomyocyte ferroptosis in ischemia-reperfusion mice via the Pum2/PRDX6 axis

Author:
Jian-Kai Zhang; Zhi Zhang; Zi-Ao Guo; Yuan Fu; Xiao-Jia Chen; Wei-Jie Chen; Hong-Fu Wu; Xiao-Jun Cui
Source:
Nutrition, metabolism, and cardiovascular diseases 2022 v.32 no.2 pp. 515-527
ISSN:
0939-4753
Subject:
bone marrow; cardiac output; cardiomyocytes; cell proliferation; chromatin immunoprecipitation; exosomes; ferroptosis; genes; glutathione; hypoxia; metabolism; mice; nutrition; peroxiredoxin; reactive oxygen species
Abstract:
... The exosomal long noncoding RNAs (lncRNAs) have been reported to have cardioprotective effects on ischemia-reperfusion (I/R) injury by hindering ferroptosis, but the role of lncRNA Mir9-3 host gene (Mir9-3hg) in cardiac I/R injury remains unclear.Exosomes were extracted from mouse bone marrow mesenchymal stem cells (BMSCs) and identified by detecting the exosome specific marker levels, and the res ...
DOI:
10.1016/j.numecd.2021.10.017

16. Benchmarking a Microfluidic-Based Filtration for Isolating Biological Particles

Author:
Fatih Inci
Source:
Langmuir 2022 v.38 no.5 pp. 1897-1909
ISSN:
1520-5827
Subject:
blood serum; diagnostic techniques; drugs; exosomes; humans; kidneys; medicine; microfiltration; models; precision medicine; tissue culture
Abstract:
... Isolating particles from complex fluids is a crucial approach in multiple fields including biomedicine. In particular, biological matrices contain a myriad of distinct particles with different sizes and structures. Extracellular vesicles (EVs), for instance, are nanosized particles carrying vital information from donor to recipient cells, and they have garnered significant impact on disease diagno ...
DOI:
10.1021/acs.langmuir.1c03119

17. Biomarkers of gastric cancer: current advancement

Author:
Tiantian Jiang; Lin Mei; Xiao Yang; Tingkai Sun; Zhidong Wang; Yuanyuan Ji
Source:
Heliyon 2022 pp. e10899
ISSN:
2405-8440
Subject:
DNA; biomarkers; blood serum; early diagnosis; exosomes; liquids; microRNA; morbidity; mortality; prediction; prognosis; stomach neoplasms; therapeutics; East Asia
Abstract:
... Gastric cancer (GC) is one of the most prevalent malignant types worldwide, especially in East Asia. Due to its frequently advanced stage at diagnosis, the mortality from GC is high and the prognosis is still unsatisfactory. Thus, early detection using effective screening approaches is vital to decrease the morbidity and mortality of GC. Interestingly, biomarkers can be used for diagnosis, predict ...
DOI:
10.1016/j.heliyon.2022.e10899

18. Bone marrow mesenchymal stem cells-derived exosomal microRNA-16-5p restrains epithelial-mesenchymal transition in breast cancer cells via EPHA1/NF-κB signaling axis

Author:
Yuan Zhang; Xiaofeng Lai; Qingfang Yue; Fei Cao; Yue Zhang; Yang Sun; Jun Tian; Yizhao Lu; Li He; Jun Bai; Yifang Wei
Source:
Genomics 2022 v.114 no.3 pp. 110341
ISSN:
0888-7543
Subject:
bone marrow; breast neoplasms; carcinogenesis; exosomes; gene overexpression; genomics; mice; xenotransplantation
Abstract:
... This study intends to conquer the mystery of microRNA-16-5p/erythropoietin-producing hepatocellular A1/nuclear factor-κB signaling (miR-16-5p/EPHA1/NF-κB signaling) in breast cancer. Expression of miR-16-5p, EPHA1 and NF-κB signaling-related proteins were detected. Gene overexpression or silencing was used to examine the biological roles of bone marrow mesenchymal stem cells (BMSCs)-derived exo-mi ...
DOI:
10.1016/j.ygeno.2022.110341

19. Bone marrow‐mesenchymal stem cell‐derived extracellular vesicles affect proliferation and apoptosis of leukemia cells in vitro

Author:
Jitrada Phetfong; Tulyapruek Tawonsawatruk; Witchayapon Kamprom; Pawared Ontong; Dalina Tanyong; Suparerk Borwornpinyo; Aungkura Supokawej
Source:
FEBS Open Bio 2022 v.12 no.2 pp. 470-479
ISSN:
2211-5463
Subject:
apoptosis; carcinogenesis; cell cycle; cell differentiation; cell growth; cell proliferation; death domain receptors; exosomes; gene expression; gene expression regulation; genes; immune system; leukemia; molecular weight; therapeutics; ultrafiltration
Abstract:
... Mesenchymal stem cells (MSCs) have been proposed to have potential for tissue engineering and cell therapy due to their multilineage differentiation potential and ability to secrete numerous paracrine factors, including extracellular vesicles (EVs). Increasing evidence has demonstrated that MSC‐derived EVs (MSC‐EVs) are able to induce the repair of tissue damage and regulate the immune system. How ...
DOI:
10.1002/2211-5463.13352

20. Boosting extracellular vesicle secretion

Author:
Lior Debbi; Shaowei Guo; Dina Safina; Shulamit Levenberg
Source:
Biotechnology advances 2022 v.59 pp. 107983
ISSN:
0734-9750
Subject:
COVID-19 infection; biotechnology; exosomes; secretion; spinal cord; tissue repair
Abstract:
... In recent years, extracellular vesicles (EVs), specifically exosomes, have emerged as a promising strategy for treating a wide spectrum of pathologies, such as cancer and COVID-19, as well as promoting tissue regeneration in various conditions, including cardiomyopathies and spinal cord injuries. Despite the great potential of EV-based therapies, poor yield and unscalable production of EVs remain ...
DOI:
10.1016/j.biotechadv.2022.107983

21. A CAR T-inspiring platform based on antibody-engineered exosomes from antigen-feeding dendritic cells for precise solid tumor therapy

Author:
Miao Fan; Huifang Liu; Hongyu Yan; Ruijun Che; Yi Jin; Xinjian Yang; Xiaohan Zhou; Hua Yang; Kun Ge; Xing-Jie Liang; Jinchao Zhang; Zhenhua Li
Source:
Biomaterials 2022 v.282 pp. 121424
ISSN:
0142-9612
Subject:
T-lymphocytes; antigens; biocompatible materials; crosslinking; exosomes; neoplasm cells; neoplasms; therapeutics; transportation
Abstract:
... Chimeric antigen receptor T (CAR T) cell therapy has achieved remarkable results treating patients with hematological malignancies in clinical studies. Although promising, extensive research has also revealed that CAR T therapy is unsatisfactory for the treatment of solid tumors. In addition, the production of CAR T cells is time-consuming and it's hard for storage and transportation. In this work ...
DOI:
10.1016/j.biomaterials.2022.121424

22. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains

Author:
Carla Prezioso; Sara Passerini; Dolores Limongi; Anna Teresa Palamara; Ugo Moens; Valeria Pietropaolo
Source:
Viruses 2022 v.14 no.9 pp. -
ISSN:
1999-4915
Subject:
DNA; Polyomaviridae; exosomes; microRNA; transcription (genetics); viral load; virus replication
Abstract:
... Since the non-coding control region (NCCR) and microRNA (miRNA) could represent two different and independent modalities of regulating JC polyomavirus (JCPyV) replication at the transcriptional and post-transcriptional levels, the interplay between JC viral load based on NCCR architecture and miRNA levels, following JCPyV infection with archetypal and rearranged (rr)-NCCR JCPyV variants, was explo ...
DOI:
10.3390/v14092070

23. Cardioprotection of M2 macrophages-derived exosomal microRNA-24-3p/Tnfsf10 axis against myocardial injury after sepsis

Author:
XingCheng Sun; Yuee Liu; Jin Wang; Min Zhang; Meitang Wang
Source:
Molecular immunology 2022 v.141 pp. 309-317
ISSN:
0161-5890
Subject:
apoptosis; blood serum; cardiac output; cardiomyocytes; cardioprotective effect; exosomes; inflammation; intraperitoneal injection; lipopolysaccharides; models; tumor necrosis factors
Abstract:
... Some reports have suggested the involvement of microRNA-24-3p (miR-24-3p) in heart diseases. Here, the intention of this work was to unmask whether miR-24-3p from M2 macrophages-derived exosomes (M2-exo) could protect against myocardial injury after sepsis.Mice model of sepsis was induced by intraperitoneal injection of lipopolysaccharide (LPS). miR-24-3p and tumor necrosis factor superfamily memb ...
DOI:
10.1016/j.molimm.2021.11.003

24. Catalytic hairpin assembly as cascade nucleic acid circuits for fluorescent biosensor: Design, evolution and application

Author:
Zewei Luo; Yongxin Li; Pei Zhang; Lu He; Yanting Feng; Yaqiang Feng; Cheng Qian; Yonghui Tian; Yixiang Duan
Source:
Trends in analytical chemistry 2022 v.151 pp. 116582
ISSN:
0165-9936
Subject:
analytical chemistry; biosensors; evolution; exosomes; fluorescence; nucleic acids
Abstract:
... Catalytic hairpin assembly (CHA) with advantages of high efficiency, flexible design, and mild reaction condition is considered a promising molecular amplifier. Modified working principle of CHAs has been coupled with multiples signal generation and signal transductions to develop the diversified fluorescent biosensors. Emerging signal amplification techniques were introduced into CHA to meet the ...
DOI:
10.1016/j.trac.2022.116582

25. Characterization of plasma-derived exosomal miRNA changes following traffic-related air pollution exposure: A randomized, crossover trial based on small RNA sequencing

Author:
Xihao Du; Qingli Zhang; Yixuan Jiang; Xinlei Zhu; Yang Zhang; Cong Liu; Yue Niu; Jing Cai; Renjie Chen; Haidong Kan
Source:
Environment international 2022 v.167 pp. 107430
ISSN:
0160-4120
Subject:
air; air pollution; carbon; carbon dioxide; cross-over studies; cytokines; droplets; environment; environmental exposure; exosomes; immune response; microRNA; nitrogen dioxide; sequence analysis; China
Abstract:
... The underlying mechanisms for health effects of traffic-related air pollution (TRAP) are still unclear. Small RNA sequencing (RNA-seq) in exosomes represents as a powerful approach to elucidate biological pathways in response to environmental exposure. We therefore aimed to explore impact of TRAP exposure on exosomal miRNAs. We performed a randomized, crossover study among 35 healthy college stude ...
DOI:
10.1016/j.envint.2022.107430

26. The Chinese herbal formula QiangGuYin regulates the Wnt/β-catenin pathway in osteoblasts by intervening in the expression of miRNAs derived from osteoclast exosomes

Author:
Binbin Tang; Yifeng Yuan; Shen Wang; Shaohua Li; Shifa Kang; Caijian He; Hang Zhou; Mouhao Fang; Tianpeng Chen; Kang Liu; Xiaolin Shi
Source:
Pharmacological research 2022 v.3 pp. 100087
ISSN:
2667-1425
Subject:
Western blotting; blood serum; bone formation; electron microscopy; exosomes; microRNA; osteoblasts; osteoclasts; research; secretion
Abstract:
... To explore the effects of a TCM compound QiangGuYin (QGY) regulating osteoclast derived exosomal miRNAs on the Wnt/β-catenin pathway in osteoclast and osteoblasts. QGY containing serum was prepared to construct osteogenic and osteoclastic differentiation models. Setting up a non-medicated and a medicated serum group with intervention on osteoclast differentiation after extraction of exosomes, the ...
DOI:
10.1016/j.prmcm.2022.100087

27. Chitosan hydrogel, as a biological macromolecule-based drug delivery system for exosomes and microvesicles in regenerative medicine: a mini review

Author:
Lan Guo; Yali Guan; Peng Liu; Linghuan Gao; Zhifu Wang; Shengli Huang; Liang Peng; Zhiyu Zhao
Source:
Cellulose 2022 v.29 no.3 pp. 1315-1330
ISSN:
0969-0239
Subject:
cellulose; chitosan; drug delivery systems; exosomes; hydrogels; injection site; medicine
Abstract:
... Extracellular vesicles are membrane-packed entities that are involved in various physiological and pathological processes. Recently, the role of exosomes and microvesicles in regenerative medicine has been elucidated. Indeed, therapeutic potential of stem cells may be partly due to exosomes or microvesicles. However, injection of these vesicles may lead to their rapid clearance from the injection ...
DOI:
10.1007/s10570-021-04330-7

28. Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression

Author:
William Garland; Iris Müller; Mengjun Wu; Manfred Schmid; Katsutoshi Imamura; Leonor Rib; Albin Sandelin; Kristian Helin; Torben Heick Jensen
Source:
Molecular cell 2022 v.82 no.9 pp. 1691-1707.e8
ISSN:
1097-2765
Subject:
chromatin; epigenetics; exosomes; humans; mutagens; transcription (genetics); transposons
Abstract:
... Transposable elements (TEs) are widespread genetic parasites known to be kept under tight transcriptional control. Here, we describe a functional connection between the mouse-orthologous “nuclear exosome targeting” (NEXT) and “human silencing hub” (HUSH) complexes, involved in nuclear RNA decay and the epigenetic silencing of TEs, respectively. Knocking out the NEXT component ZCCHC8 in embryonic s ...
DOI:
10.1016/j.molcel.2022.03.004

29. CircFBXW8 Acts an Oncogenic Role in the Malignant Progression of Non-small Cell Lung Carcinoma by miR-370-3p-Dependent Regulation of TRIM44

Author:
Xia Wang; Jian Lv; Bin He; Deqi Zhou
Source:
Biochemical genetics 2022 v.60 no.4 pp. 1313-1332
ISSN:
0006-2928
Subject:
WD40 repeats; Western blotting; apoptosis; cell proliferation; cell viability; circular RNA; exosomes; flow cytometry; immunohistochemistry; lung neoplasms; migratory behavior; protein synthesis; quantitative polymerase chain reaction; secretion; xenotransplantation
Abstract:
... Non-small cell lung carcinoma (NSCLC) is an aggressive malignant tumor. Growing evidences have revealed that circular RNA (circRNA) is involved in NSCLC progression. This study aims to investigate the role of circular RNA F-box and WD repeat domain containing 8 (circFBXW8) in NSCLC progression and the underlying mechanism. The expression of circFBXW8, microRNA-370-3p (miR-370-3p) and tripartite mo ...
DOI:
10.1007/s10528-021-10177-1

30. CircPLK1 Acts as a Carcinogenic Driver to Promote the Development of Malignant Pleural Mesothelioma by Governing the miR-1294/HMGA1 Pathway

Author:
Qi Zhang; Zhiqiang Wang; Huarong Cai; Dongming Guo; Wei Xu; Shi Bu; Yuequan Jiang
Source:
Biochemical genetics 2022 v.60 no.5 pp. 1527-1546
ISSN:
0006-2928
Subject:
Western blotting; animals; bioluminescence assay; blood serum; breast neoplasms; carcinogenesis; carcinogenicity; cell movement; cell proliferation; cell viability; exosomes; mesothelioma; nanoparticles; neoplasm cells; quantitative polymerase chain reaction; transmission electron microscopy
Abstract:
... The deregulation of circular RNAs (circRNAs) is involved in cancer development. CircRNA polo-like kinase 1 (circPLK1) was reported to promote breast cancer development. However, the role of circPLK1 in malignant pleural mesothelioma (MPM) is unclear. The expression of circPLK1, miR-1294, and high mobility group AT-hook 1 (HMGA1) mRNA was measured by quantitative real-time PCR (qPCR). Cell viabilit ...
DOI:
10.1007/s10528-022-10186-8

31. Circulating exosome miRNA, is it the novel nutrient molecule through cross-kingdom regulation mediated by food chain transmission from microalgae to bivalve?

Author:
Zhe Zheng; Zhijie Xu; Caixia Cai; Yongshan Liao; Chuangye Yang; Xiaodong Du; Ronglian Huang; Yuewen Deng
Source:
Comparative biochemistry and physiology 2022 v.43 pp. 101004
ISSN:
1744-117X
Subject:
Nannochloropsis; Pinctada fucata; apoptosis; biomineralization; endocytosis; energy metabolism; exosomes; food chain; gene expression; hemolymph; mammals; microRNA; microalgae; oysters; sequence analysis; signal transduction
Abstract:
... MicroRNAs (miRNAs) can efficiently regulate gene expression at intracellular and extracellular levels. Plant-derived miRNAs are highly enriched in animal haemolymph and regulate mammalian gene expression. However, evidence for food-derived miRNAs in Mollusca species is lacking. In this study, we fed the microalga Nannochloropsis oculata to the pearl oyster Pinctada fucata martensii and detected di ...
DOI:
10.1016/j.cbd.2022.101004

32. Circulating extracellular vesicles and rheumatoid arthritis: a proteomic analysis

Author:
Mohammed Alghamdi; Sultan Abdulmughni Alamry; Sami M. Bahlas; Vladimir N. Uversky; Elrashdy M. Redwan
Source:
Cellular and molecular life sciences 2022 v.79 no.1 pp. 25
ISSN:
1420-682X
Subject:
apoptosis; autoantigens; autoimmunity; exosomes; human physiology; immunosuppression; inflammation; prognosis; proteomics; rheumatoid arthritis; therapeutics; transportation
Abstract:
... Circulating extracellular vesicles (EVs) are membrane-bound nanoparticles secreted by most cells for intracellular communication and transportation of biomolecules. EVs carry proteins, lipids, nucleic acids, and receptors that are involved in human physiology and pathology. EV cargo is variable and highly related to the type and state of the cellular origin. Three subtypes of EVs have been identif ...
DOI:
10.1007/s00018-021-04020-4

33. Clodronate-nintedanib-loaded exosome–liposome hybridization enhances the liver fibrosis therapy by inhibiting Kupffer cell activity

Author:
Keqin Ji; Mingrui Fan; Dong Huang; Lingna Sun; Bingqin Li; Ruoting Xu; Jiajing Zhang; Xuan Shao; Yanzuo Chen
Source:
Biomaterials science 2022 v.10 no.3 pp. 702-713
ISSN:
2047-4849
Subject:
biocompatible materials; cytokines; drug delivery systems; drugs; exosomes; fibroblasts; fibrosis; hybridization; inflammation; liver; liver cirrhosis; mice; phagocytosis
Abstract:
... Liver fibrosis therapy remains limited due to the inefficiency of drug delivery and inflammation induced by Kupffer cells. In this study, an exosome–liposome hybrid drug delivery system (LIEV) was developed to increase the efficacy of clodronate (CLD)-inhibition of Kupffer cells and to effectively deliver nintedanib (NIN) to liver fibroblasts to ensure enhanced anti-fibrosis therapy. CLD and NIN c ...
DOI:
10.1039/d1bm01663f

34. Comparative analysis of exosomal miRNAs derived from lipopolysaccharide and polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line

Author:
Yeojin Hong; Thi Hao Vu; Sooyeon Lee; Jubi Heo; Suyeon Kang; Hyun S. Lillehoj; Yeong Ho Hong
Source:
Poultry science 2022 v.101 no.11 pp. 102141
ISSN:
0032-5791
Subject:
cell lines; chickens; exosomes; gene expression; lipopolysaccharides; macrophages; microRNA; mitogen-activated protein kinase; polyinosinic-polycytidylic acid
Abstract:
... Exosomes play important roles in cellular communication by delivering exosomal proteins and nucleic acid molecules to cells. In particular, exosomal miRNAs can modulate various biological processes in recipient cells by repressing target gene expression. In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of ...
DOI:
10.1016/j.psj.2022.102141

35. Controlled Release of Exosomes Using Atom Transfer Radical Polymerization-Based Hydrogels

Author:
Saigopalakrishna S. Yerneni; Sushil Lathwal; Julia Cuthbert; Kriti Kapil; Grzegorz Szczepaniak; Jaepil Jeong; Subha R. Das; Phil G. Campbell; Krzysztof Matyjaszewski
Source:
Biomacromolecules 2022 v.23 no.4 pp. 1713-1722
ISSN:
1526-4602
Subject:
DNA; blood; bone formation; bone morphogenetic proteins; crosslinking; drugs; exosomes; hydrogels; intravenous injection; lipids; polymerization
Abstract:
... Exosomes are 30–200 nm sized extracellular vesicles that are increasingly recognized as potential drug delivery vehicles. However, exogenous exosomes are rapidly cleared from the blood upon intravenous delivery, which limits their therapeutic potential. Here, we report bioactive exosome-tethered poly(ethylene oxide)-based hydrogels for the localized delivery of therapeutic exosomes. Using choleste ...
DOI:
10.1021/acs.biomac.1c01636

36. Coupling suspension trapping–based sample preparation and data-independent acquisition mass spectrometry for sensitive exosomal proteomic analysis

Author:
Ci Wu; Shiyun Zhou; Megan I. Mitchell; Chunyan Hou; Stephen Byers; Olivier Loudig; Junfeng Ma
Source:
Analytical and bioanalytical chemistry 2022 v.414 no.8 pp. 2585-2595
ISSN:
1618-2642
Subject:
analytical chemistry; breast neoplasms; breasts; epithelium; exosomes; mass spectrometry; peptides; proteomics
Abstract:
... It has been a challenge to analyze minute amounts of proteomic samples in a facile and robust manner. Herein, we developed a quantitative proteomics workflow by integrating suspension trapping (S-Trap)–based sample preparation and label-free data-independent acquisition (DIA) mass spectrometry and then applied it for the analysis of microgram and even nanogram amounts of exosome samples. S-Trap–ba ...
DOI:
10.1007/s00216-022-03920-z

37. Cryptic erythrocytic infections in Plasmodium vivax, another challenge to its elimination

Author:
Carmen Fernandez-Becerra; Iris Aparici-Herraiz; Hernando A. del Portillo
Source:
Parasitology international 2022 v.87 pp. 102527
ISSN:
1383-5769
Subject:
Plasmodium vivax; antimalarials; bone marrow; exosomes; geographical distribution; humans; immune response; liver; malaria; parasitology; people; risk; spleen; Asia
Abstract:
... Human malaria caused by Plasmodium vivax infection (vivax malaria) is a major global health issue. It is the most geographically widespread form of the disease, accounting for 7 million annual clinical cases, the majority of cases in America and Asia and an estimation of over 2.5 billion people living under risk of infection. The general perception towards vivax malaria has shifted recently, follo ...
DOI:
10.1016/j.parint.2021.102527

38. Cubic DNA nanocage-based three-dimensional molecular beacon for accurate detection of exosomal miRNAs in confined spaces

Author:
Dongsheng Mao; Mengmeng Zheng; Wenxing Li; Yi Xu; Chengguang Wang; Qiuling Qian; Shuainan Li; Guifang Chen; Xiaoli Zhu; Xianqiang Mi
Source:
Biosensors & bioelectronics 2022 v.204 pp. 114077
ISSN:
0956-5663
Subject:
DNA; biosensors; electrostatic interactions; energy transfer; exosomes; fluorescence; microRNA
Abstract:
... In situ nondestructive bioanalysis of targets in nanoscale confined space, e.g. exosomes, poses a high challenge to analytical technologies, especially to molecular fluorescent probes, because it is required to enter the confined space to recognize the target, and maintain independent and stable signal output. The unexpected fluorescence quenching and fluorescence resonance energy transfer (FRET) ...
DOI:
10.1016/j.bios.2022.114077

39. Current knowledge and the future potential of extracellular vesicles in mammalian reproduction

Author:
Dawit Tesfaye; Nico Menjivar; Samuel Gebremedhn
Source:
Reproduction, fertility, and development 2022 v.34 no.2 pp. 174-189
ISSN:
1031-3613
Subject:
DNA; apoptosis; biomarkers; blastocyst; cell communication; cryopreservation; endometritis; exosomes; mammals; microRNA; oocytes; oviducts
Abstract:
... Extracellular vesicles (EVs), which contain various functional classes of vesicles, namely exosomes, microvesicles, and apoptotic bodies, represent the major nano-shuttle to transfer bioactive molecules from donor to recipient cells to facilitate cell-to-cell communication in the follicular, oviduct, and uterine microenvironments. In addition to transferring various molecular cargos in the form of ...
DOI:
10.1071/RD21277

40. Current status and outlook of advances in exosome isolation

Author:
Wen-zhao Liu; Zhan-jun Ma; Xue-wen Kang
Source:
Analytical and bioanalytical chemistry 2022 v.414 no.24 pp. 7123-7141
ISSN:
1618-2642
Subject:
analytical chemistry; biomarkers; cell communication; exosomes; isolation techniques
Abstract:
... Exosomes are extracellular vesicles with a diameter ranging from 30 to 150 nm, which are an important medium for intercellular communication and are closely related to the progression of certain diseases. Therefore, exosomes are considered promising biomarkers for the diagnosis of specific diseases, and thereby, treatments based on exosomes are being widely examined. For exosome-related research, ...
DOI:
10.1007/s00216-022-04253-7

41. DNA Zipper Mediated Membrane Fusion for Rapid Exosomal MiRNA Detection

Author:
Miao Xie; Fujun Wang; Jiapei Yang; Yuanyuan Guo; Fei Ding; Xinmiao Lu; Yangyang Huang; Yimeng Li; Xinyuan Zhu; Chuan Zhang
Source:
Analytical chemistry 2022 v.94 no.38 pp. 13043-13051
ISSN:
1520-6882
Subject:
DNA; analytical chemistry; biopsy; cell culture; culture media; early diagnosis; exosomes; flow cytometry; fluorescence; lipids; membrane fusion; microRNA; neoplasms; patients
Abstract:
... Accurate and reliable detection of exosomal miRNA can serve as a promising method for early diagnosis of disease and evaluation of therapeutic effects. However, current exosomal miRNA detection methods commonly involve exosome enrichment, containing RNA extraction, and qRT-PCR based quantification, which are expensive and time-consuming. Herein, we develop a DNA zipper-mediated membrane fusion app ...
DOI:
10.1021/acs.analchem.2c01980

42. DNA barcode-based detection of exosomal microRNAs using nucleic acid lateral flow assays for the diagnosis of colorectal cancer

Author:
Seokjoon Kim; Jinjoo Han; Jung Soo Park; Jung Ho Kim; Eun Sung Lee; Byung Seok Cha; Ki Soo Park
Source:
Talanta 2022 v.242 pp. 123306
ISSN:
0039-9140
Subject:
DNA; biopsy; colorectal neoplasms; colorimetry; endoscopy; exosomes; microRNA; reverse transcription
Abstract:
... Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer-related deaths worldwide. The standard methods for diagnosing CRC, endoscopy and tissue biopsy, are invasive and time-consuming. Herein, we propose a novel method for the accurate and non-invasive diagnosis of CRC based on the analysis of exosomes that are circulating in biological fluids using a DNA bar ...
DOI:
10.1016/j.talanta.2022.123306

43. Delivery of siRNA based on engineered exosomes for glioblastoma therapy by targeting STAT3

Author:
Shi-Fu Liang; Fang-Fang Zuo; Bin-Cheng Yin; Bang-Ce Ye
Source:
Biomaterials science 2022 v.10 no.6 pp. 1582-1590
ISSN:
2047-4849
Subject:
biocompatible materials; blood; blood circulation; blood-brain barrier; brain; exosomes; glioblastoma; immunogenicity; prognosis; xenotransplantation
Abstract:
... Small interfering RNA (siRNA) therapy has been considered as a promising strategy for treatment of glioblastoma (GBM), which is an aggressive brain disease with poor prognosis. However, siRNA therapy for GBM is seriously hindered by a multitude of barriers including possible immunogenicity, poor cellular uptake, short blood circulation, poor blood stability and low blood–brain barrier (BBB) penetr ...
DOI:
10.1039/d1bm01723c

44. Depletion of serum-derived exosomes aggravates heat stress-induced damage of bovine mammary epithelial cells

Author:
Yue Wang; Hui-Li Wang; Zhi-Ping Lin; Ji-Feng Zhong; Kun-Lin Chen; Xing Duan
Source:
Molecular biology reports 2022 v.49 no.10 pp. 9297-9305
ISSN:
0301-4851
Subject:
apoptosis; cattle; cell communication; epithelium; exosomes; fetal bovine serum; heat; heat stress; membrane potential; milk fat; milk synthesis; mitochondria; mitochondrial membrane; molecular biology; reactive oxygen species; superoxide dismutase; viability
Abstract:
... BACKGROUND: Exosomes are involved in intercellular communication, affecting many physiological and pathological process. The present study evaluated the effects of serum exosomes on the function of bovine mammary epithelial cells (BMECs) and milk synthesis under heat stress. METHODS AND RESULTS: We cultured the BMECs in fetal bovine serum (FBS) or exosome-free FBS medium and examined, their viabil ...
DOI:
10.1007/s11033-022-07767-6

45. Design and development of novel fluorescence sensing material for exosome recognition

Author:
Qianjin Hua; Yan Jin; Gang Wei; Wei Wang; Linyou Wang; Yu Yin; Jun Yang; Yuanlong Gu; Caifang Ni
Source:
Colloids and surfaces 2022 v.214 pp. 112421
ISSN:
0927-7765
Subject:
biocompatibility; cell viability; chemical structure; cytotoxicity; erythrocytes; exosomes; fluorescence; hemolysis; hydrogen; hydrophilicity; neoplasms; polymers; toxicity testing; transmembrane proteins
Abstract:
... Exosomes are extracellular vesicles with relatively specific expression of CD63 transmembrane protein. In this study, We designed and constructed a multisite-targeting polymer which has both fluorescence and targeting recognition. It can bond to the hydrophilic group of CD63 by connecting with hydrogen. The chemical structure and the ability to combine with CD63 of fluorescent monomer and polymer ...
DOI:
10.1016/j.colsurfb.2022.112421

46. Detecting early-stage malignant melanoma using a calcium switch-enriched exosome subpopulation containing tumor markers as a sample

Author:
Da-Yeon Choi; Ji-Na Park; Sung-Ho Paek; Seung-Cheol Choi; Se-Hwan Paek
Source:
Biosensors & bioelectronics 2022 v.198 pp. 113828
ISSN:
0956-5663
Subject:
antibody specificity; biosensors; calcium; exosomes; magnetic separation; melanoma; monoclonal antibodies
Abstract:
... An exosome species containing CD63 as a marker of melanoma was isolated from bulk exosome population and used as a sample for detecting malignant melanoma. A calcium binding protein (CBP) was produced and then used to raise monoclonal antibody. The antibody was sensitive to a conformational change of CBP caused by Ca²⁺ binding. Immuno-magnetic beads were prepared by immobilizing the conformation-s ...
DOI:
10.1016/j.bios.2021.113828

47. Detection of multiplex exosomal miRNAs for clinically accurate diagnosis of Alzheimer’s disease using label-free plasmonic biosensor based on DNA-Assembled advanced plasmonic architecture

Author:
Sojin Song; Jong Uk Lee; Myeong Jin Jeon; Soohyun Kim; Sang Jun Sim
Source:
Biosensors & bioelectronics 2022 v.199 pp. 113864
ISSN:
0956-5663
Subject:
biomarkers; biosensors; blood serum; cognitive disorders; energy density; exosomes; humans; microRNA; nanorods; refractive index; surface plasmon resonance
Abstract:
... Alzheimer’s disease (AD), the most common neurologic disorder, is characterized by progressive cognitive impairment. However, the low clinical significance of the currently used core AD biomarkers amyloid-beta and tau proteins remains a challenge. Recently, exosomes, found in human biological fluids, are gaining increasing attention because of their clinical significance in diagnosing of various d ...
DOI:
10.1016/j.bios.2021.113864

48. Development of a high-pH reversed-phase well plate for peptide fractionation and deep proteome analysis of cells and exosomes

Author:
Yu-Ching Liu; Yu-Ting Huang; Chao-Jung Chen
Source:
Analytical and bioanalytical chemistry 2022 v.414 no.7 pp. 2513-2522
ISSN:
1618-2642
Subject:
analytical chemistry; exosomes; fractionation; peptides; proteome; proteomics
Abstract:
... The complexity of the proteome often limits the number of identified proteins in the nanoflow LC-MS (nanoLC-MS) analysis of samples. Therefore, peptide fractionation is essential for reducing the sample complexity and improving the proteome coverage. In this study, to achieve high-pH reversed-phase (RP)-well plate fractionation for high-throughput proteomics analysis, C18 particles were coated on ...
DOI:
10.1007/s00216-022-03892-0

49. Differences in the secretory exosomes of Clonorchis sinensis adults at different incubation times

Author:
Xiaoli Zhang; Shanshan Duan; Xiang Li; Jian Ding; Lijiao Zuo; Beibei Sun; Xueli Zhang; Xu Jiang; Yannan Gao; Xinyi Hu; Su Han
Source:
Acta tropica 2022 v.234 pp. 106604
ISSN:
0001-706X
Subject:
Clonorchis sinensis; culture media; exosomes; immune system; microRNA; pathogenesis; rats; reverse transcriptase polymerase chain reaction; signal transduction; transmission electron microscopy; ultracentrifugation
Abstract:
... Exosomes are small membrane vesicles of endocytic origin and widely involved in a variety of physiological and pathological conditions. Exosome-like vesicles (ELVs) have been identified to mediate the parasite-host interactions and communication. Thus, increased knowledge of C. sinensis ELVs could provide insights into parasite-host interactions. In this experiment, ELVs was purified by ultracentr ...
DOI:
10.1016/j.actatropica.2022.106604

50. Differential expression and sorting of exosomal microRNAs upon activation of the human monocyte-like cell line U937

Author:
Song-Yi Choi; Su-Hyung Hong; Heon-Jin Lee
Source:
Biochemical and biophysical research communications 2022 v.610 pp. 147-153
ISSN:
0006-291X
Subject:
cell communication; eukaryotic cells; exosomes; gene expression regulation; humans; macrophages; mass spectrometry; microRNA; research
Abstract:
... Extracellular vesicles such as exosomes in eukaryotes have drawn scrutiny due to their various roles in intercellular communication. Small RNAs, including microRNAs (miRNAs), are more abundant among the cargo of exosomes than other RNA types. MiRNAs loaded in secreted exosomes (or extracellular microRNAs) can be transported to recipient cells and may play a regulatory role although the miRNA loadi ...
DOI:
10.1016/j.bbrc.2022.04.048