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1. Enterohemorrhagic Escherichia coli (EHEC) in water from karst springs: detection with real-time PCR and isolation of strains

Author:
Andreas Baumgartner; Isabel Niederhauser; David Diston; Dominik Moor
Source:
Journal für Verbraucherschutz und Lebensmittelsicherheit 2016 v.11 no.4 pp. 353-357
ISSN:
1661-5751
Subject:
Shiga-like toxins; enterohemorrhagic Escherichia coli; food safety; foods; genes; intimin; karsts; quantitative polymerase chain reaction; springs (water); virulence; virulent strains; Switzerland
Abstract:
... Within 2 months, two water sources in a karst area in Switzerland were sampled 9 times each, and analyzed by real-time PCR for 6 EHEC O-types, Shiga-like-toxin (stx1 and stx2) and intimin (eae) genes. With the exception of O111, 5 O-types were recorded regularly and at high frequencies (O26: 33.3 %; O157: 33.3 %; O104: 66.6 %; O103: 72.2 %; O145: 94.4 %). Genes for Shiga-like-toxins and intimin we ...
DOI:
10.1007/s00003-016-1053-1