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You searched for: Subject genetic techniques and protocols Remove constraint Subject: genetic techniques and protocols Subject term insect pests Remove constraint Subject term: insect pests Subject term genetic techniques and protocols Remove constraint Subject term: genetic techniques and protocols
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1. Genetic analysis of oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) populations based on mitochondrial cox1 and nad1 gene sequences from India and other Asian countries

Author:
Jaipal S. Choudhary; Naiyar Naaz; Chandra S. Prabhakar; Moanaro Lemtur
Source:
Genetica 2016 v.144 no.5 pp. 611-623
ISSN:
0016-6707
Subject:
Bactrocera dorsalis; NADH dehydrogenase; cytochrome-c oxidase; demography; fruit crops; genes; genetic techniques and protocols; genetic variation; haplotypes; insect pests; mitochondria; nucleotide sequences; species diversity; sterile insect technique; India
Abstract:
... The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destructive and polyphagous insect pest of fruit crops in diverse geographic regions of India. 19 widely dispersed populations of the fly from India and other Asian countries were analysed using partial sequences of mitochondrial cytochrome oxidase I (cox1) and NADH dehydrogenase 1 (nad1) genes to investigat ...
DOI:
10.1007/s10709-016-9929-7

2. Modeling seasonal migration of fall armyworm moths

Author:
J. K. Westbrook; R. N. Nagoshi; R. L. Meagher; S. J. Fleischer; S. Jairam
Source:
International journal of biometeorology 2016 v.60 no.2 pp. 255-267
ISSN:
0020-7128
Subject:
Spodoptera frugiperda; bioclimatology; climate change; cultivars; fate and transport models; flight; freezing point; genetic techniques and protocols; genetically modified organisms; haplotypes; host plants; host range; immigration; insect pests; migratory behavior; moths; pheromone traps; population ecology; prediction; simulation models; spring; summer; temperate zones; Florida; Ontario; Quebec; Texas
Abstract:
... Fall armyworm, Spodoptera frugiperda (J.E. Smith), is a highly mobile insect pest of a wide range of host crops. However, this pest of tropical origin cannot survive extended periods of freezing temperature but must migrate northward each spring if it is to re-infest cropping areas in temperate regions. The northward limit of the winter-breeding region for North America extends to southern regions ...
DOI:
10.1007/s00484-015-1022-x

3. Genetic control of invasive fish: technological options and its role in integrated pest management

Author:
Ronald E. Thresher; Keith Hayes; Nicholas J. Bax; John Teem; Tillmann J. Benfey; Fred Gould
Source:
Biological invasions 2014 v.16 no.6 pp. 1201-1216
ISSN:
1387-3547
Subject:
biological control; fish; genes; genetic techniques and protocols; insect pests; integrated pest management; invasive species; issues and policy; males; models; sterile insect technique; triploidy; United States
Abstract:
... Genetic options for the control of invasive fishes were recently reviewed and synthesized at a 2010 international symposium, held in Minneapolis/St. Paul, MN, USA. The only option currently available “off-the-shelf” is triploidy, which can be used to produce sterile males for a release program analogous to those widely and successfully used for biological control of insect pests. However, the Troj ...
DOI:
10.1007/s10530-013-0477-0

4. crw1 - A Novel Maize Mutant Highly Susceptible to Foliar Damage by the Western Corn Rootworm Beetle

Author:
Bala Puchakayala Venkata; Nick Lauter; Xu Li; Clint Chapple; Christian Krupke; Gurmukh Johal; Stephen Moose; Mingliang Xu
Source:
PloS one v.8 no.8 pp. -
ISSN:
1932-6203
Subject:
Diabrotica virgifera virgifera; Zea mays; adults; cell wall components; cell walls; corn; epidermis (plant); genetic techniques and protocols; hosts; insect pests; leaves; mutants; mutation; phenolic acids; pollen; silk
Abstract:
... Western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is the most destructive insect pest of corn (Zea mays L.) in the United States. The adult WCR beetles derive their nourishment from multiple sources including corn pollen and silks as well as the pollen of alternate hosts. Conversely, the corn foliage is largely neglected as a food source by WCR beetle ...
DOI:
10.1371/journal.pone.0071296
PubMed:
23951124
PubMed Central:
PMC3739800

5. Cross resistance, genetic analysis and stability of resistance to buprofezin in cotton whitefly, Bemisia tabaci (Homoptera: Aleyrodidae)

Author:
Muhammad Basit; Mushtaq A. Saleem; Shafqat Saeed; Ali H. Sayyed
Source:
Crop protection 2012 v.40 no. pp. 16-21
ISSN:
0261-2194
Subject:
Bemisia tabaci; acetamiprid; at-risk population; buprofezin; cross resistance; genetic techniques and protocols; imidacloprid; insect pests; pyriproxyfen; reciprocal crosses
Abstract:
... Whitefly, Bemisia tabaci (Gennadius), is an economically important insect pest that has developed resistance to several classes of insecticides. Our study was designed to investigate crossresistance and genetics of buprofezin resistance in field collected population of B. tabaci. At generation one (G₁), a low level of resistance to buprofezin, pyriproxyfen, spirotetramat, imidacloprid and acetamip ...
DOI:
10.1016/j.cropro.2012.03.024

6. Ecology of Rickettsia felis: A Review

Author:
Reif, Kathryn E.; Macaluso, Kevin R.
Source:
Journal of medical entomology 2009 v.46 no.4 pp. 723-736
ISSN:
0022-2585
Subject:
geographical distribution; host range; insect pests; ectoparasites; hosts; emerging diseases; Rickettsia felis; animal pathogenic bacteria; disease transmission; insect vectors; microbial ecology; Ctenocephalides felis; genetic techniques and protocols; signs and symptoms (animals and humans)
Abstract:
... It has been two decades since the first description of Rickettsia felis, and although a nearly cosmopolitan distribution is now apparent, much of the ecology of this unique microorganism remains unresolved. The cat flea, Ctenocephalides felis, is currently the only known biological vector of R. felis; however, molecular evidence of R. felis in other species of fleas as well as in ticks and mites s ...
DOI:
10.1603/033.046.0402
PubMed:
19645274

7. Are lice good proxies for host history? A comparative analysis of the Australian magpie, Gymnorhina tibicen, and two species of feather louse

Author:
Toon, A.; Hughes, J.M.
Source:
Heredity 2008 v.101 no.2 pp. 127-135
ISSN:
0018-067X
Subject:
insect pests; coevolution; hosts; geographical variation; phylogeny; Passeriformes; Philopteridae; wild birds; population genetics; genetic techniques and protocols; lice; habitats; biogeography
Abstract:
... Parasites have been recently advocated as useful proxies for unravelling a complex evolutionary history of a host. In the present study, two species of feather lice, Brueelia semiannulata and Philopterus sp. were analysed for mitochondrial variation and compared to mitochondrial and nuclear variation in their host, the Australian magpie Gymnorhina tibicen. Phylogenies constructed using maximum lik ...
DOI:
10.1038/hdy.2008.37
PubMed:
18461081

8. High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta

Author:
Rahman, Md Lutfor; Jiang, Wenzhu; Chu, Sang Ho; Qiao, Yongli; Ham, Tae-Ho; Woo, Mi-Ok; Lee, Joohyun; Khanam, M. Sakina; Chin, Joong-Hyoun; Jeung, Ji-Ung; Brar, D. S.; Jena, K. K.; Koh, Hee-Jong
Source:
Theoretical and applied genetics 2009 v.119 no.7 pp. 1237-1246
ISSN:
0040-5752
Subject:
Nilaparvata lugens; Oryza minuta; brown rice; cultivars; genes; genetic techniques and protocols; insect pests; introgression; loci; quantitative trait loci
Abstract:
... Brown planthopper (BPH) is one of the most destructive insect pests of rice. Wild species of rice are a valuable source of resistance genes for developing resistant cultivars. A molecular marker-based genetic analysis of BPH resistance was conducted using an F₂ population derived from a cross between an introgression line, ‘IR71033-121-15', from Oryza minuta (Accession number 101141) and a suscept ...
DOI:
10.1007/s00122-009-1125-z
PubMed:
19669727

9. High throughput tissue preparation for large-scale genotyping experiments

Author:
SLOTTA, TRACEY A. BODO; BRADY, LEE; CHAO, SHIAOMAN
Source:
Molecular ecology resources 2008 v.8 no.1 pp. 83-87
ISSN:
1755-098X
Subject:
plant tissues; insect pests; DNA; genetic techniques and protocols; extraction; silica gel; crops
Abstract:
... A fast, efficient technique is described for the extraction of DNA from a large number of samples. The applications of this method include population genetics, plant breeding, and genetic screening. In the field, samples are collected in premeasured silica gel aliquots in polypropylene blocks, which are later used to grind the dried tissue. This permits naturalists, breeders, and collaborators to ...
Handle:
10113/32698
DOI:
10.1111/j.1471-8286.2007.01907.x
PubMed:
21585722

10. Identification of potential detoxification enzyme genes in Leptinotarsa decemlineata (Say) and study of their expression in insects reared on different plants

Author:
Zhang, Jianhua; Pelletier, Yvan; Goyer, Claudia
Source:
Canadian journal of plant science 2008 v.88 no.4 pp. 621-629
ISSN:
0008-4220
Subject:
Leptinotarsa decemlineata; insect pests; plant pests; metabolic detoxification; enzyme activity; gene expression; cytochrome P-450; protein synthesis; host plants; allelochemicals; glutathione transferase; esterases; expressed sequence tags; complementary DNA; genetic techniques and protocols; host range; Solanum lycopersicum var. lycopersicum; tomatoes; Capsicum annuum; Solanum melongena; eggplants; Nicotiana benthamiana; biochemical pathways
DOI:
10.4141/CJPS07001

11. Mitochondrial DNA Restriction Map and Cytochrome c Oxidase Subunits I and II Sequence Divergence of Corn Stalk Borer Sesamia nonagrioides (Lepidoptera: Noctuidae)

Author:
Kourti, Anna
Source:
Biochemical genetics 2006 v.44 no.7-8 pp. 320-331
ISSN:
0006-2928
Subject:
Sesamia nonagrioides; cytochrome-c oxidase; genes; genetic techniques and protocols; genetic variation; geographical distribution; haplotypes; insect pests; mitochondrial DNA; nucleotide sequences; phylogeny; Greece; Mediterranean region; Spain
Abstract:
... Corn stalk borer Sesamia nonagrioides (Lefebvre) (Lepidoptera: Noctuidae) is among the most important insect pests of corn in the Mediterranean basin. The mitochondrial DNA of this insect was purified and a restriction map was constructed. The size of the mtDNA genome is 16.3 kb. Genetic analysis of four corn stalk borer populations, collected from Greece (three populations) and Spain (one populat ...
DOI:
10.1007/s10528-006-9032-3

12. Improving Access to the Control Region and tRNA Gene Clusters of Dipteran Mitochondrial DNA

Author:
Oliveira, M.T.; Da Rosa, A.C.; Azeredo-Espin, A.M.L.; Lessinger, A.C.
Source:
Journal of medical entomology 2006 v.43 no.3 pp. 636-639
ISSN:
0022-2585
Subject:
Diptera; insect pests; mitochondrial DNA; transfer RNA; nucleotide sequences; genes; DNA primers; genetic techniques and protocols
Abstract:
... Despite the successful use of universal primers for amplifying insect mtDNA, specific regions remain difficult to recover and demand the use of taxon-specific primers. In this work, we describe a new set of primers for efficiently amplifying and sequencing the mtDNA control region and three tRNA gene clusters of dipterans of medical and veterinary importance, including Muscidae, Calliphoridae, and ...
DOI:
10.1603/0022-2585(2006)43[636:IATTCR]2.0.CO;2
PubMed:
16739428

13. Systemic response to aphid infestation by Myzus persicae in the phloem of Apium graveolens

Author:
Divol, F.; Vilaine, F.; Thibivilliers, S.; Amselem, J.; Palauqui, J.C.; Kusiak, C.; Dinant, S.
Source:
Plant molecular biology 2005 v.57 no.4 pp. 517-540
ISSN:
0167-4412
Subject:
messenger RNA; insect pests; celery; host-parasite relationships; Myzus persicae; expressed sequence tags; gene expression; phloem; resistance mechanisms; cDNA libraries; genetic techniques and protocols; gene induction; pest resistance; Apium graveolens
Abstract:
... Little is known about the molecular processes involved in the phloem response to aphid feeding. We investigated molecular responses to aphid feeding on celery (Apium graveolens cv. Dulce) plants infested with the aphid Myzus persicae, as a means of identifying changes in phloem function. We used celery as our model species as it is easy to separate the phloem from the surrounding tissues in the pe ...
PubMed:
15821978

14. The players in a mutualistic symbiosis: insects, bacteria, viruses, and virulence genes

Author:
Moran, N.A.; Degnan, P.H.; Santos, S.R.; Dunbar, H.E.; Ochman, H.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2005 v.102 no.47 pp. 16919-16926
ISSN:
0027-8424
Subject:
insect pests; Acyrthosiphon pisum; endosymbionts; bacteriophages; amino acid sequences; mutualism; hemolymph; genes; nucleotide sequences; genetic techniques and protocols; type III secretion system; toxins; virulence; bacteria
Abstract:
... Aphids maintain mutualistic symbioses involving consortia of coinherited organisms. All possess a primary endosymbiont, Buchnera, which compensates for dietary deficiencies; many also contain secondary symbionts, such as Hamiltonella defensa, which confers defense against natural enemies. Genome sequences of uncultivable secondary symbionts have been refractory to analysis due to the difficulties ...
DOI:
10.1073/pnas.0507029102
PubMed:
16195380
PubMed Central:
PMC1287993

15. Mitochondrial diversity evaluated by the single strand conformation polymorphism method in African and North American house flies (Musca domestica L.)

Author:
Marguez, J.G.; Krafsur, E.S.
Source:
Insect molecular biology 2003 v.12 no.2 pp. 99-106
ISSN:
0962-1075
Subject:
insect pests; loci; genetic polymorphism; haplotypes; mitochondrial DNA; geographical variation; genetic distance; gene flow; gene frequency; genetic techniques and protocols; genetic variation; population structure; Musca domestica; North America; Sub-Saharan Africa
DOI:
10.1046/j.1365-2583.2003.00391.x
PubMed:
12653931

16. Biological and molecular properties of a begomovirus from Dicliptera sexangularis

Author:
Lotrakul, P.; Valverde, R.A.; Landry, A.D.
Source:
Phytopathology 2000 v.90 no.7 pp. 723-729
ISSN:
0031-949X
Subject:
Acanthaceae; Geminiviridae; pathogenicity; host range; disease vectors; Bemisia tabaci; insect pests; phylogeny; genetic techniques and protocols; nucleotide sequences; Florida
DOI:
10.1094/PHYTO.2000.90.7.723
PubMed:
18944491

17. Trade-offs between pathogen and herbivore resistance

Author:
Felton, G.W.; Korth, K.L.
Source:
Current opinion in plant biology 2000 v.3 no.4 pp. 309-314
ISSN:
1369-5266
Subject:
insect pests; plant pathogens; signal transduction; herbivores; biochemistry; disease resistance; genetic techniques and protocols; plant physiology; pest resistance
PubMed:
10873851

18. Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis

Author:
Reymond, P.; Weber, H.; Damond, M.; Farmer, E.E.
Source:
Plant cell 2000 v.12 no.5 pp. 707-719
ISSN:
1040-4651
Subject:
Arabidopsis thaliana; complementary DNA; genetic techniques and protocols; gene expression; messenger RNA; defense mechanisms; mechanical damage; insect pests; larvae; Pieris rapae; water stress; leaves; jasmonic acid; ethylene
PubMed:
10810145
PubMed Central:
PMC139922

19. Tomato chlorosis virus: a new whitefly-transmitted, phloem-limited, bipartite closterovirus of tomato

Author:
Wisler, G.C.; Li, R.H.; Liu, H.Y.; Lowry, D.S.; Duffus, J.E.
Source:
Phytopathology 1998 v.88 no.5 pp. 402-409
ISSN:
0031-949X
Subject:
Solanum lycopersicum var. lycopersicum; Nicotiana clevelandii; Closterovirus; disease transmission; Trialeurodes vaporariorum; Trialeurodes abutiloneus; Bemisia tabaci; Bemisia argentifolii; insect pests; pathogenicity; phloem; pathotypes; genetic techniques and protocols; RNA; nucleotide sequences; genetic variation; Tomato infectious chlorosis virus
Abstract:
... Tomato chlorosis virus (ToCV) is the second whitefly-transmitted, phloem-limited, bipartite closterovirus described infecting tomato. ToCV is distinct from tomato infectious chlorosis virus (TICV), based on lack of serological and nucleic acid cross-reactions and differences in vector specificity. TICV is transmitted only by the greenhouse whitefly (Trialeurodes vaporariorum), whereas ToCV is tran ...
DOI:
10.1094/PHYTO.1998.88.5.402
PubMed:
18944918

20. An insecticidal N-acetylglucosamine-specific lectin gene from Griffonia simplicifolia (Leguminosae)

Author:
Zhu, K.; Huesing, J.E.; Shade, R.E.; Bressan, R.A.; Hasegawa, P.M.; Murdock, L.L.
Source:
Plant physiology 1996 v.110 no.1 pp. 195-202
ISSN:
0032-0889
Subject:
genes; pest resistance; Griffonia simplicifolia; clones; gene expression; leaves; defense mechanisms; insect pests; seeds; complementary DNA; genetic code; insecticidal properties; amino acid sequences; Callosobruchus maculatus; glucosamine; glycosylation; lectins; binding sites; nucleotide sequences; genetic techniques and protocols; chemical constituents of plants
Abstract:
... Griffonia simplicifolia II, an N-acetylglucosamine-specific legume lectin, has insecticidal activity when fed to the cowpea weevil, Callosobruchus maculatus (F.). A cDNA clone encoding G. simplicifolia II was isolated from a leaf cDNA library, sequenced, and expressed in a bacterial expression system. The recombinant protein exhibited N-acetylglucosamine-binding and insecticidal activity against c ...
PubMed:
8587982
PubMed Central:
PMC157709