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- Author:
- Hanna, Jacob; Wernig, Marius; Markoulaki, Styliani; Sun, Chiao-Wang; Meissner, Alexander; Cassady, John P.; Beard, Caroline; Brambrink, Tobias; Wu, Li-Chen; Townes, Tim M.; Jaenisch, Rudolf
- Source:
- Science 2007 v.318 no.5858 pp. 1920-1923
- ISSN:
- 0036-8075
- Subject:
- Retroviridae; abnormal hemoglobin; alleles; animal disease models; autografting; cell transplantation; disease control; fibroblasts; gene targeting; genetic vectors; hematopoietic stem cells; humans; induced pluripotent stem cells; mice; oncogenes; sickle cell anemia; transcription (genetics); transcription factors
- Abstract:
- ... It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell-like state by introducing combinations of four transcription factors. However, the therapeutic potential of such induced pluripotent stem (iPS) cells remained undefined. By using a humanized sickle cell anemia mouse model, we show that mice can be rescued after transplantation with he ...
- DOI:
- 10.1126/science.1152092
- https://doi.org/10.1126/science.1152092
- Author:
- Lowry, W.E.; Richter, L.; Yachechko, R.; Pyle, A.D.; Tchieu, J.; Sridharan, R.; Clark, A.T.; Plath, K.
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.8 pp. 2883-2888
- ISSN:
- 0027-8424
- Subject:
- DNA fingerprinting; cell fusion; embryonic stem cells; fibroblasts; gene expression; humans; induced pluripotent stem cells; somatic cells; transcription factors
- Abstract:
- ... The generation of patient-specific pluripotent stem cells has the potential to accelerate the implementation of stem cells for clinical treatment of degenerative diseases. Technologies including somatic cell nuclear transfer and cell fusion might generate such cells but are hindered by issues that might prevent them from being used clinically. Here, we describe methods to use dermal fibroblasts ea ...
- DOI:
- 10.1073/pnas.0711983105
- PubMed:
- 18287077
- PubMed Central:
- PMC2268554
- https://doi.org/10.1073/pnas.0711983105
- Author:
- Stadtfeld, Matthias; Nagaya, Masaki; Utikal, Jochen; Weir, Gordon; Hochedlinger, Konrad
- Source:
- Science 2008 v.322 no.5903 pp. 945-949
- ISSN:
- 0036-8075
- Subject:
- Adenoviridae; DNA; chimerism; embryonic stem cells; fibroblasts; genes; germ cells; hepatocytes; humans; induced pluripotent stem cells; insertional mutagenesis; mice; somatic cells; transcription (genetics); transcription factors; viruses
- Abstract:
- ... Pluripotent stem cells have been generated from mouse and human somatic cells by viral expression of the transcription factors Oct4, Sox2, Klf4, and c-Myc. A major limitation of this technology is the use of potentially harmful genome-integrating viruses. We generated mouse induced pluripotent stem (iPS) cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expres ...
- DOI:
- 10.1126/science.1162494
- PubMed:
- 18818365
- PubMed Central:
- PMC3987909
- https://doi.org/10.1126/science.1162494
- Author:
- Dimos, John T.; Rodolfa, Kit T.; Niakan, Kathy K.; Weisenthal, Laurin M.; Mitsumoto, Hiroshi; Chung, Wendy; Croft, Gist F.; Saphier, Genevieve; Leibel, Rudy; Goland, Robin; Wichterle, Hynek; Henderson, Christopher E.; Eggan, Kevin
- Source:
- Science 2008 v.321 no.5893 pp. 1218-1221
- ISSN:
- 0036-8075
- Subject:
- amyotrophic lateral sclerosis; chronic diseases; drugs; elderly; embryonic stem cells; fibroblasts; humans; induced pluripotent stem cells; models; motor neurons; patients; women
- Abstract:
- ... The generation of pluripotent stem cells from an individual patient would enable the large-scale production of the cell types affected by that patient's disease. These cells could in turn be used for disease modeling, drug discovery, and eventually autologous cell replacement therapies. Although recent studies have demonstrated the reprogramming of human fibroblasts to a pluripotent state, it rema ...
- DOI:
- 10.1126/science.1158799
- https://doi.org/10.1126/science.1158799
- Author:
- Ezashi, Toshihiko; Telugu, Bhanu Prakash V.L.; Alexenko, Andrei P.; Sachdev, Shrikesh; Sinha, Sunilima; Roberts, R. Michael
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.27 pp. 10993-10998
- ISSN:
- 0027-8424
- Subject:
- blood serum; cultured cells; embryonic stem cells; fibroblasts; humans; induced pluripotent stem cells; karyotyping; mice; microarray technology; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; safety testing; somatic cells; swine; telomerase; transcription (genetics); transgenes
- Abstract:
- ... For reasons that are unclear the production of embryonic stem cells from ungulates has proved elusive. Here, we describe induced pluripotent stem cells (iPSC) derived from porcine fetal fibroblasts by lentiviral transduction of 4 human (h) genes, hOCT4, hSOX2, hKLF4, and hc-MYC, the combination commonly used to create iPSC in mouse and human. Cells were cultured on irradiated mouse embryonic fibro ...
- DOI:
- 10.1073/pnas.0905284106
- PubMed:
- 19541600
- PubMed Central:
- PMC2698893
- https://doi.org/10.1073/pnas.0905284106
- Author:
- Sun, Ning; Panetta, Nicholas J.; Gupta, Deepak M.; Wilson, Kitchener D.; Lee, Andrew; Jia, Fangjun; Hu, Shijun; Cherry, Athena M.; Robbins, Robert C.; Longaker, Michael T.; Wu, Joseph C.
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.37 pp. 15720-15725
- ISSN:
- 0027-8424
- Subject:
- adults; biopsy; fibroblasts; humans; induced pluripotent stem cells; patients; somatic cells; transcription factors
- Abstract:
- ... Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients ...
- DOI:
- 10.1073/pnas.0908450106
- PubMed:
- 19805220
- PubMed Central:
- PMC2739869
- https://doi.org/10.1073/pnas.0908450106
- Author:
- Beqqali, A.; van Eldik, W.; Mummery, C.; Passier, R.
- Source:
- Cellular and molecular life sciences 2009 v.66 no.5 pp. 800-813
- ISSN:
- 1420-682X
- Subject:
- cardiomyocytes; cardiovascular diseases; embryonic stem cells; human development; humans; induced pluripotent stem cells; medicine; models; therapeutics
- Abstract:
- ... Studies on identification, derivation and characterization of human stem cells in the last decade have led to high expectations in the field of regenerative medicine. Although it is clear that for successful stem cell-based therapy several obstacles have to be overcome, other opportunities lay ahead for the use of human stem cells. A more immediate application would be the development of human mod ...
- DOI:
- 10.1007/s00018-009-8476-0
- https://doi.org/10.1007/s00018-009-8476-0
- Author:
- Shevchenko, A. I.; Medvedev, S. P.; Mazurok, N. A.; Zakian, S. M.
- Source:
- Russian journal of genetics 2009 v.45 no.2 pp. 139-146
- ISSN:
- 1022-7954
- Subject:
- embryonic stem cells; genes; humans; induced pluripotent stem cells; mice; somatic cells
- Abstract:
- ... Induced pluripotent stem cells (iPS) result from a reprogramming of somatic cells via transduction with viral vectors expressing the Oct4, Sox2, c-Myc, Klf4, Nanog, and Lin28 genes, which are essential for the establishment and maintenance of the pluripotent state. In properties, iPS are almost fully similar to embryonic stem cells (ESC). To date, iPS have been obtained from various differentiated ...
- DOI:
- 10.1134/S1022795409020021
- https://doi.org/10.1134/S1022795409020021
- Author:
- Ramzey Abujarour; Sheng Ding
- Source:
- Genome biology 2009 v.10 no.5 pp. 2102
- ISSN:
- 1474-760X
- Subject:
- genes; humans; induced pluripotent stem cells; mice; somatic cells
- Abstract:
- ... The development of novel approaches for reprogramming mouse and human somatic cells has enabled the generation of induced pluripotent stem cells that are free of exogenous genes. ...
- DOI:
- 10.1186/gb-2009-10-5-220
- PubMed:
- 19439061
- PubMed Central:
- PMC2718510
- https://doi.org/10.1186/gb-2009-10-5-220
- Author:
- Meyer, Jason S.; Shearer, Rebecca L.; Capowski, Elizabeth E.; Wright, Lynda S.; Wallace, Kyle A.; McMillan, Erin L.; Zhang, Su-Chun; Gamm, David M.
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.39 pp. 16698-16703
- ISSN:
- 0027-8424
- Subject:
- embryonic stem cells; gene expression; human development; humans; induced pluripotent stem cells; models; ontogeny; retina
- Abstract:
- ... Human pluripotent stem cells have the potential to provide comprehensive model systems for the earliest stages of human ontogenesis. To serve in this capacity, these cells must undergo a targeted, stepwise differentiation process that follows a normal developmental timeline. Here we demonstrate the ability of both human embryonic stem cells (hESCs) and induced pluripotent stem (iPS) cells to meet ...
- DOI:
- 10.1073/pnas.0905245106
- PubMed:
- 19706890
- PubMed Central:
- PMC2757802
- https://doi.org/10.1073/pnas.0905245106
- Author:
- Nimmo, Rachael A.; Slack, Frank J.
- Source:
- Chromosoma 2009 v.118 no.4 pp. 405-418
- ISSN:
- 0009-5915
- Subject:
- Caenorhabditis elegans; adults; breast neoplasms; cell cycle; fibroblasts; genes; humans; imaginal discs; induced pluripotent stem cells; larvae; liver neoplasms; microRNA
- Abstract:
- ... MicroRNAs (miRNAs) were first discovered in genetic screens for regulators of developmental timing in the stem-cell-like seam cell lineage in Caenorhabditis elegans. As members of the heterochronic pathway, the lin-4 and let-7 miRNAs are required in the seam cells for the correct progression of stage-specific events and to ensure that cell cycle exit and terminal differentiation occur at the corre ...
- DOI:
- 10.1007/s00412-009-0210-z
- PubMed:
- 19340450
- PubMed Central:
- PMC4322900
- https://doi.org/10.1007/s00412-009-0210-z
- Author:
- Hargus, Gunnar; Cooper, Oliver; Deleidi, Michela; Levy, Adam; Lee, Kristen; Marlow, Elizabeth; Yow, Alyssa; Soldner, Frank; Hockemeyer, Dirk; Hallett, Penelope J.; Osborn, Teresia; Jaenisch, Rudolf; Isacson, Ole
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.36 pp. 15921-15926
- ISSN:
- 0027-8424
- Subject:
- Parkinson disease; adults; animal models; autografting; axons; biomedical research; brain; cell adhesion; humans; induced pluripotent stem cells; patients; rats
- Abstract:
- ... Recent advances in deriving induced pluripotent stem (iPS) cells from patients offer new possibilities for biomedical research and clinical applications, as these cells could be used for autologous transplantation. We differentiated iPS cells from patients with Parkinson's disease (PD) into dopaminergic (DA) neurons and show that these DA neurons can be transplanted without signs of neurodegenerat ...
- DOI:
- 10.1073/pnas.1010209107
- PubMed:
- 20798034
- PubMed Central:
- PMC2936617
- https://doi.org/10.1073/pnas.1010209107
- Author:
- Miyoshi, Keiko; Tsuji, Daisuke; Kudoh, Keiko; Satomura, Kazuhito; Muto, Taro; Itoh, Kohji; Noma, Takafumi
- Source:
- Journal of bioscience and bioengineering 2010 v.110 no.3 pp. 345-350
- ISSN:
- 1389-1723
- Subject:
- aesthetics; biopsy; fibroblasts; gene transfer; humans; induced pluripotent stem cells; mucosa; patients; tissue repair
- Abstract:
- ... Induced pluripotent stem (iPS) cells are one of the most promising sources for cell therapy in regenerative medicine. Using a patient's own genetically identical and histocompatible cells is the ideal way to practice personalized regenerative medicine. For personalized iPS cell therapy, the prerequisites for cell source preparation are a simple and safe procedure, no aesthetic or functional damage ...
- DOI:
- 10.1016/j.jbiosc.2010.03.004
- https://doi.org/10.1016/j.jbiosc.2010.03.004
14. Generation of induced pluripotent stem cells using site-specific integration with phage integrase
- Author:
- Ye, Lin; Chang, Judy C.; Lin, Chin; Qi, Zhongxia; Yu, Jingwei; Kan, Yuet Wai
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.45 pp. 19467-19472
- ISSN:
- 0027-8424
- Subject:
- surface antigens; induced pluripotent stem cells; epigenetics; transcription factors; loci; humans; fibroblasts; site-specific recombination; reverse transcriptase polymerase chain reaction; bacteriophages; somatic cells; gene expression; mice; microRNA; genes; intergenic DNA; amniotic fluid
- Abstract:
- ... To date, a large number of reports have described reprogramming many somatic cell types into induced pluripotent stem (iPS) cells, using different numbers of transcription factors and devising alternate methods of introducing the transcription factor genes or proteins into the somatic cells. Here, we describe a method using bacteriophage ΦC31 integrase to reprogram mouse embryonic fibroblasts and ...
- DOI:
- 10.1073/pnas.1012677107
- PubMed:
- 20974949
- PubMed Central:
- PMC2984165
- https://doi.org/10.1073/pnas.1012677107
- Author:
- Moreno-Gimeno, Inmaculada; Ledran, Maria H.; Lako, Majlinda
- Source:
- FEBS journal 2010 v.277 no.24 pp. 5014-5025
- ISSN:
- 1742-464X
- Subject:
- adults; coculture; embryonic stem cells; hematologic diseases; hematopoiesis; hematopoietic stem cells; humans; immunotherapy; induced pluripotent stem cells; medicine; models; stromal cells
- Abstract:
- ... Human embryonic stem cells (hESCs) and induced pluripotent stem cells are excellent models for the study of embryonic hematopoiesis in vitro, aiding the design of new differentiation models that may be applicable to cell-replacement therapies. Adult and fetal hematopoietic stem cells are currently being used in biomedical applications; however, the latest advances in regenerative medicine and stem ...
- DOI:
- 10.1111/j.1742-4658.2010.07926.x
- https://doi.org/10.1111/j.1742-4658.2010.07926.x
16. High Resolution Array-CGH Characterization of Human Stem Cells Using a Stem Cell Focused Microarray
- Author:
- Elliott, Aaron M.; Hohenstein Elliott, Kristi A.; Kammesheidt, Anja
- Source:
- Molecular biotechnology 2010 v.46 no.3 pp. 234-242
- ISSN:
- 1073-6085
- Subject:
- chromosome aberrations; genes; genetic stability; humans; induced pluripotent stem cells; karyotyping; microarray technology; nucleic acid hybridization
- Abstract:
- ... Human embryonic and induced pluripotent stem cells (ESCs, iPSCs) that are cultured for an extended period of time are susceptible to genomic instability. Chromosomal aberrations decrease the reliability and reproducibility of experiments and could deem the cells unusable for therapeutic purposes. The genetic stability of human ESCs and iPSCs is commonly monitored by karyotype analysis. However, th ...
- DOI:
- 10.1007/s12033-010-9294-1
- https://doi.org/10.1007/s12033-010-9294-1
- Author:
- Nizzardo, M.; Simone, C.; Falcone, M.; Locatelli, F.; Riboldi, G.; Comi, G. P.; Corti, S.
- Source:
- Cellular and molecular life sciences 2010 v.67 no.22 pp. 3837-3847
- ISSN:
- 1420-682X
- Subject:
- embryonic stem cells; humans; induced pluripotent stem cells; motor neurons; nervous system diseases; neuroprotective effect; somatic cells
- Abstract:
- ... Motor neuron diseases (MNDs) are a group of neurological disorders that selectively affect motor neurons. There are currently no cures or efficacious treatments for these diseases. In recent years, significant developments in stem cell research have been applied to MNDs, particularly regarding neuroprotection and cell replacement. However, a consistent source of motor neurons for cell replacement ...
- DOI:
- 10.1007/s00018-010-0463-y
- https://doi.org/10.1007/s00018-010-0463-y
- Author:
- Chamberlain, Stormy J.; Chen, Pin-Fang; Ng, Khong Y.; Bourgois-Rocha, Fany; Lemtiri-Chlieh, Fouad; Levine, Eric S.; Lalande, Marc
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.41 pp. 17668-17673
- ISSN:
- 0027-8424
- Subject:
- DNA methylation; alleles; brain; gene expression regulation; genetic disorders; genomic imprinting; human diseases; humans; induced pluripotent stem cells; models; neurons; patients; ubiquitin-protein ligase
- Abstract:
- ... Angelman syndrome (AS) and Prader-Willi syndrome (PWS) are neurodevelopmental disorders of genomic imprinting. AS results from loss of function of the ubiquitin protein ligase E3A (UBE3A) gene, whereas the genetic defect in PWS is unknown. Although induced pluripotent stem cells (iPSCs) provide invaluable models of human disease, nuclear reprogramming could limit the usefulness of iPSCs from patie ...
- DOI:
- 10.1073/pnas.1004487107
- PubMed:
- 20876107
- PubMed Central:
- PMC2955112
- https://doi.org/10.1073/pnas.1004487107
- Author:
- Hu, Bao-Yang; Weick, Jason P.; Yu, Junying; Ma, Li-Xiang; Zhang, Xiao-Qing; Thomson, James A.; Zhang, Su-Chun
- Source:
- Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.9 pp. 4335-4340
- ISSN:
- 0027-8424
- Subject:
- cell transplantation; embryonic stem cells; humans; induced pluripotent stem cells; screening; transcription (genetics); transgenes
- Abstract:
- ... For the promise of human induced pluripotent stem cells (iPSCs) to be realized, it is necessary to ask if and how efficiently they may be differentiated to functional cells of various lineages. Here, we have directly compared the neural-differentiation capacity of human iPSCs and embryonic stem cells (ESCs). We have shown that human iPSCs use the same transcriptional network to generate neuroepith ...
- DOI:
- 10.1073/pnas.0910012107
- PubMed:
- 20160098
- PubMed Central:
- PMC2840097
- https://doi.org/10.1073/pnas.0910012107
- Author:
- Laprise, Shari L.
- Source:
- Molecular biotechnology 2010 v.45 no.2 pp. 187-197
- ISSN:
- 1073-6085
- Subject:
- assisted reproductive technologies; embryonic stem cells; genomic imprinting; humans; induced pluripotent stem cells; patients; risk; therapeutics
- Abstract:
- ... The “holy grail” of regenerative medicine is the identification of an undifferentiated progenitor cell that is pluripotent, patient specific, and ethically unambiguous. Such a progenitor cell must also be able to differentiate into functional, transplantable tissue, while avoiding the risks of immune rejection. With reports detailing aberrant genomic imprinting associated with assisted reproductiv ...
- DOI:
- 10.1007/s12033-010-9252-y
- https://doi.org/10.1007/s12033-010-9252-y