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You searched for: Subject immobilized enzymes Remove constraint Subject: immobilized enzymes Subject coordination polymers Remove constraint Subject: coordination polymers
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1. Novel trypsin–FITC@MOF bioreactor efficiently catalyzes protein digestion

Author:
Wan-Ling Liu; Sheng-Han Lo; Brenda Singco; Chun-Chuen Yang; Hsi-Ya Huang; Chia-Her Lin
Source:
Journal of materials chemistry B 2013 v.1 no.7 pp. 928-932
ISSN:
2050-7518
Subject:
bioreactors; catalytic activity; coordination polymers; energy; immobilized enzymes; nanopores; organic wastes; proteolysis; trypsin
Abstract:
... A simple nanoporous metal organic framework (MOF) bioreactor preparation via a low energy 30 minute vortex as driving force to immobilize trypsin enzyme is presented. No chemical modifications were employed thus organic wastes were eliminated. The digestion efficiency of this trypsin bioreactor is exceptionally high, even when reused. This “green” synthesis procedure produced a “green” bioreactor. ...
DOI:
10.1039/c3tb00257h

2. MOF-templated rough, ultrathin inorganic microcapsules for enzyme immobilization

Author:
Xiaoli Wang; Jiafu Shi; Shaohua Zhang; Hong Wu; Zhongyi Jiang; Chen Yang; Yuxin Wang; Lei Tang; Anfu Yan
Source:
Journal of materials chemistry B 2015 v.3 no.32 pp. 6587-6598
ISSN:
2050-7518
Subject:
EDTA (chelating agent); biomimetics; calcium carbonate; coordination polymers; crystals; enzymatic reactions; hydrophobicity; immobilized enzymes; mineralization; penicillin amidase; porosity; storage quality; surface roughness; tannins; titanium dioxide
Abstract:
... Ultrathin titania microcapsules with rough surfaces were prepared by using a metal–organic framework (ZIF-8) as one kind of hard template to mediate the structures of the microcapsule shell. Specifically, CaCO₃ particles were first coated with tannic acid (TA) followed by the deposition of hydrophobic ZIF-8 and another TA layer, the obtained particles were then assembled with protamine/TiO₂ bilaye ...
DOI:
10.1039/c5tb00870k

3. Construction of Thermophilic Lipase-Embedded Metal–Organic Frameworks via Biomimetic Mineralization: A Biocatalyst for Ester Hydrolysis and Kinetic Resolution

Author:
He Hongming; Han Haobo; Shi Hui; Tian Yuyang; Sun Fuxing; Song Yang; Li Quanshun; Zhu Guangshan
Source:
ACS Applied Materials & Interfaces 2016 v.8 no.37 pp. 24517-24524
ISSN:
1944-8252
Subject:
biocatalysis; biocatalysts; biomimetics; carboxylic ester hydrolases; chemical elements; coordination polymers; enantiomers; hydrolysis; immobilized enzymes; mineralization; models; zeolites
Abstract:
... Enhancing the activity and stability of enzymes and improving their reusability are critical challenges in the field of enzyme immobilization. Here we report a facile and efficient biomimetic mineralization to embed thermophilic lipase QLM in zeolite imidazolate framework-8 (ZIF-8). Systematic characterization indicated that the entrapment of lipase molecules was successfully achieved during the c ...
DOI:
10.1021/acsami.6b05538

4. Microperoxidase-11/metal–organic framework/macroporous carbon for detecting hydrogen peroxide

Author:
Coucong Gong; Jingyi Chen; Yuan Shen; Yonggui Song; Yonghai Song; Li Wang
Source:
RSC advances 2016 v.6 no.83 pp. 79798-79804
ISSN:
2046-2069
Subject:
active sites; biosensors; carbon; coordination polymers; electrochemistry; electrodes; energy-dispersive X-ray analysis; hydrogen peroxide; immobilized enzymes; kenaf; nanogold; nanoparticles; porous media; scanning electron microscopy
Abstract:
... In this work, a novel hydrogen peroxide (H₂O₂) electrochemical biosensor was developed based on the metal–organic framework (MOF, Tb@mesoMOF) modified three-dimensional (3D) kenaf stem-derived macroporous carbon (3D-KSCs) electrode which was used as a new platform to load microperoxidase-11 (MP-11) for the first time. The chitosan-Au nanoparticles (CHIT-AuNPs) were firstly electrodeposited on the ...
DOI:
10.1039/c6ra16145f

5. Novel Nano-/Micro-Biocatalyst: Soybean Epoxide Hydrolase Immobilized on UiO-66-NH2 MOF for Efficient Biosynthesis of Enantiopure (R)-1, 2-Octanediol in Deep Eutectic Solvents

Author:
Cao Shi-Lin; Yue Dong-Mei; Li Xue-Hui; Smith Thomas J.; Li Ning; Zong Min-Hua; Wu Hong; Ma Yong-Zheng; Lou Wen-Yong
Source:
ACS sustainable chemistry 2016 v.4 no.6 pp. 3586-3595
ISSN:
2168-0485
Subject:
biocatalysts; biosynthesis; catalytic activity; coordination polymers; enantiomers; enzyme activity; enzyme substrates; epoxide hydrolase; glycols; hydrolysis; immobilized enzymes; pH; solvents; soybeans; thermal stability
Abstract:
... The nano-/microscale UiO-66-NH₂ metal–organic framework (MOF) materials were successfully prepared with a uniform size of about 350–400 nm and structurally characterized. Soybean epoxide hydrolase (SEH), a useful hydrolase for synthesis of valuable vicinal diols, was for the first time efficiently immobilized onto the prepared UiO-66-NH₂ MOF. The resulting novel nano-/microbiocatalyst SEH@UiO-66-N ...
DOI:
10.1021/acssuschemeng.6b00777

6. Enzyme–MOF (metal–organic framework) composites

Author:
Xizhen Lian; Yu Fang; Elizabeth Joseph; Qi Wang; Jialuo Li; Sayan Banerjee; Christina Lollar; Xuan Wang; Hong-Cai Zhou
Source:
Chemical Society reviews 2017 v.46 no.11 pp. 3386-3401
ISSN:
1460-4744
Subject:
catalytic activity; coordination polymers; enzyme activity; immobilized enzymes; synergism
Abstract:
... The ex vivo application of enzymes in various processes, especially via enzyme immobilization techniques, has been extensively studied in recent years in order to enhance the recyclability of enzymes, to minimize enzyme contamination in the product, and to explore novel horizons for enzymes in biomedical applications. Possessing remarkable amenability in structural design of the frameworks as well ...
DOI:
10.1039/c7cs00058h

7. Facile synthesis of magnetic metal organic framework for highly efficient proteolytic digestion used in mass spectrometry-based proteomics

Author:
Rui Zhai; Yifeng Yuan; Fenglong Jiao; Feiran Hao; Xiang Fang; Yangjun Zhang; Xiaohong Qian
Source:
Analytica chimica acta 2017 pp. -
ISSN:
0003-2670
Subject:
active sites; analytical chemistry; autolysis; chelating agents; coordination polymers; emus; immobilized enzymes; longevity; mass spectrometry; mice; oocytes; peptides; protein composition; proteins; proteolysis; proteome; proteomics; surface area; thermal stability; trypsin
Abstract:
... Mass spectrometry (MS) based bottom-up strategy is now the first choice for proteomics analysis. In this process, highly efficient and complete enzymatic degradation of protein samples is extremely important to achieve in-depth protein coverage and high-throughput protein profiling. However, conventional in-solution digestion suffer from long digestion time and enzyme autolysis that limit the prot ...
DOI:
10.1016/j.aca.2017.08.048

8. Immobilization on Metal–Organic Framework Engenders High Sensitivity for Enzymatic Electrochemical Detection

Author:
Zhang Cheng; Wang Xuerui; Hou Miao; Li Xiaoyang; Wu Xiaoling; Ge Jun
Source:
ACS Applied Materials & Interfaces 2017 v.9 no.16 pp. 13831-13836
ISSN:
1944-8252
Subject:
beers; biosensors; coordination polymers; cytochrome c; electrochemistry; electrodes; enzyme activity; hydrogen peroxide; immobilized enzymes; milk; porous media
Abstract:
... The protection effect of metal–organic framework (MOF) provides high stability for immobilized enzyme. The small cavities of MOFs, however, usually result in decreased apparent substrate affinity and enzymatic activity of immobilized enzyme, compared to native enzyme. We synthesized zeolitic imidazolate framework-8 (ZIF-8) with a combination of mesoporous and microporous channels for cytochrome c ...
DOI:
10.1021/acsami.7b02803

9. In situ and post‐synthesis immobilization of enzymes on nanocrystalline MOF platforms to yield active biocatalysts

Author:
Victoria Gascón; Elsa Castro‐Miguel; Manuel Díaz‐García; Rosa M Blanco; Manuel Sanchez‐Sanchez
Source:
Journal of chemical technology and biotechnology 2017 v.92 no.10 pp. 2583-2593
ISSN:
0268-2575
Subject:
ambient temperature; beta-glucosidase; biocatalysts; coordination polymers; enzyme activity; immobilized enzymes; laccase; leaching; nanocrystals; pH
Abstract:
... BACKGROUND: Very recently, metal‐organic framework (MOF) materials have been postulated as emerging supports to achieve solid‐state enzyme‐contained biocatalysts. In this work, post‐synthesis and in situ strategies to immobilize β‐glucosidase and laccase on different MOF materials were studied. The MOF‐based supports, i.e. MIL‐53(Al), NH₂‐MIL‐53(Al) and Mg‐MOF‐74, were prepared under soft and sust ...
DOI:
10.1002/jctb.5274

10. Mesoporous Metal–Organic Framework with Well-Defined Cruciate Flower-Like Morphology for Enzyme Immobilization

Author:
Cui Jiandong; Feng Yuxiao; Lin Tao; Tan Zhilei; Zhong Cheng; Jia Shiru
Source:
ACS Applied Materials & Interfaces 2017 v.9 no.12 pp. 10587-10594
ISSN:
1944-8252
Subject:
catalase; coordination polymers; crystal structure; crystals; immobilized enzymes; ions; porosity; porous media; proteins; zinc
Abstract:
... Metal–organic frameworks (MOFs) have recently emerged as a promising candidates for the immobilization of enzymes due to their diversified structures and porosity. However, a lack of good size and morphological control over the as-prepared MOFs has limited their practical applications in some cases. Herein, instead of zeolitic imidazolate framework-8 (ZIF-8) with the standard rhombic dodecahedral ...
DOI:
10.1021/acsami.7b00512

11. Synthesis and characterization of mesoporous Cu–MOF for laccase immobilization

Author:
Ziwei Zhong; Shilong Pang; Yanwen Wu; Shu Jiang; Jie Ouyang
Source:
Journal of chemical technology and biotechnology 2017 v.92 no.7 pp. 1841-1847
ISSN:
0268-2575
Subject:
adsorption; chelating agents; coordination polymers; copper; enzyme kinetics; immobilized enzymes; laccase; pH; porous media; surface area; surfactants
Abstract:
... BACKGROUND: Mesoporous metal–organic frameworks (MOFs) have been widely researched for enzyme immobilization, however, most are unstable in the aqueous phase. This paper reports the synthesis of a Cu–MOF for the immobilization of copper contained laccase via a surfactant‐templating method with various surfactant/chelating agent mass ratios. RESULTS: The Brunauer–Emmett–Teller (BET) surface area an ...
DOI:
10.1002/jctb.5189

12. Creating Hierarchical Pores by Controlled Linker Thermolysis in Multivariate Metal–Organic Frameworks

Author:
Liang Feng; Shuai Yuan; Liang-Liang Zhang; Kui Tan; Jia-Luo Li; Angelo Kirchon; Ling-Mei Liu; Peng Zhang; Yu Han; Yves J. Chabal; Hong-Cai Zhou
Source:
Journal of the American Chemical Society 2018 v.140 no.6 pp. 2363-2372
ISSN:
1520-5126
Subject:
catalytic activity; coordination polymers; crystal structure; decarboxylation; drugs; immobilized enzymes; nanoparticles; porosity; porous media; thermal degradation
Abstract:
... Sufficient pore size, appropriate stability, and hierarchical porosity are three prerequisites for open frameworks designed for drug delivery, enzyme immobilization, and catalysis involving large molecules. Herein, we report a powerful and general strategy, linker thermolysis, to construct ultrastable hierarchically porous metal–organic frameworks (HP-MOFs) with tunable pore size distribution. Lin ...
DOI:
10.1021/jacs.7b12916

13. How Do Enzymes Orient When Trapped on Metal–Organic Framework (MOF) Surfaces?

Author:
Yanxiong Pan; Hui Li; Jasmin Farmakes; Feng Xiao; Bingcan Chen; Shengqian Ma; Zhongyu Yang
Source:
Journal of the American Chemical Society 2018 v.140 no.47 pp. 16032-16036
ISSN:
1520-5126
Subject:
catalytic activity; coordination polymers; electron paramagnetic resonance spectroscopy; immobilized enzymes; leaching; trapping
Abstract:
... Enzyme immobilization in metal–organic frameworks (MOFs) offers retained enzyme integrity and activity, enhanced stability, and reduced leaching. Trapping enzymes on MOF surfaces would allow for catalysis involving large substrates. In both cases, the catalytic efficiency and selectivity depend not only on enzyme integrity/concentration but also orientation. However, it has been a challenge to det ...
DOI:
10.1021/jacs.8b09257

14. Immobilized carbonic anhydrase on mesoporous cruciate flower-like metal organic framework for promoting CO2 sequestration

Author:
Sizhu Ren; Yuxiao Feng; Huan Wen; Conghai Li; Baoting Sun; Jiandong Cui; Shiru Jia
Source:
International journal of biological macromolecules 2018 v.117 pp. 189-198
ISSN:
0141-8130
Subject:
adsorption; biocatalysts; calcium carbonate; carbon dioxide; carbon sequestration; carbonate dehydratase; carbonate minerals; coordination polymers; immobilized enzymes; ions; porous media; thermal stability; zinc
Abstract:
... CO₂ capture by immobilized carbonic anhydrase (CA) has become an alternative and environmental friendly approach in CO₂ sequestration technology. However, the immobilized CA usually exhibits low CO₂ sequestration efficiency due to no gas adsorption function for the conventional CA supports. Metal organic frameworks (MOFs) are an excellent material for gas adsorption and enzyme immobilization. Here ...
DOI:
10.1016/j.ijbiomac.2018.05.173

15. Magnetic-metal organic framework (magnetic-MOF): A novel platform for enzyme immobilization and nanozyme applications

Author:
Shamraja S. Nadar; Virendra K. Rathod
Source:
International journal of biological macromolecules 2018 v.120 pp. 2293-2302
ISSN:
0141-8130
Subject:
active sites; adsorption; biosensors; catalysts; catalytic activity; coordination polymers; encapsulation; immobilized enzymes; magnetic properties; surface area; thermal stability
Abstract:
... Enzymes are nature's catalysts which are highly selective and efficient. Recently, metal organic frameworks (MOFs) have captivated great attention as an attractive porous support matrix for immobilization of enzymes. An outstanding improvement in catalytic efficiency, enhanced chemical/thermal stability, easy accessibility to active sites, promising recyclability and high enzyme loading are some o ...
DOI:
10.1016/j.ijbiomac.2018.08.126

16. Nano-Biocatalysts of Cyt c@ZIF-8/GO Composites with High Recyclability via a de Novo Approach

Author:
Qianqian Zhu; Wei Zhuang; Yong Chen; Zhanke Wang; Byron Villacorta Hernandez; Jinglan Wu; Pengpeng Yang; Dong Liu; Chenjie Zhu; Hanjie Ying; Zhonghua Zhu
Source:
ACS applied materials & interfaces 2018 v.10 no.18 pp. 16066-16076
ISSN:
1944-8252
Subject:
acetone; biosensors; coordination polymers; crystal structure; cytochrome c; ethanol; graphene oxide; hydrogen peroxide; immobilized enzymes; moieties; nanocomposites; particle size; protein content; solvents; storage quality
Abstract:
... To improve the stability and recyclability of enzymes immobilized on metal-organic frameworks (MOFs), graphene oxide (GO) with surface oxygen-rich functional groups was selected to form ZIF-8/GO nanocomposites with the zeolitic imidazolate framework (ZIF-8) for cytochrome c (Cyt c) immobilization. It was found that the functional groups on the GO surface were involved in the growth of ZIF-8 withou ...
DOI:
10.1021/acsami.8b00072

17. Rational Design of Mimic Multienzyme Systems in Hierarchically Porous Biomimetic Metal–Organic Frameworks

Author:
Xiao Liu; Wei Qi; Yuefei Wang; Daiwu Lin; Xuejiao Yang; Rongxin Su; Zhimin He
Source:
ACS applied materials & interfaces 2018 v.10 no.39 pp. 33407-33415
ISSN:
1944-8252
Subject:
biocatalysts; biomimetics; catalytic activity; colorimetry; coordination polymers; glucose; immobilized enzymes; iron; porosity; uric acid
Abstract:
... A facile approach was reported to establish mimic multienzyme systems with hierarchically porous (HP) biomimetic metal–organic frameworks (MOFs) and natural enzymes for tandem catalysis. The hierarchically porous MOF HP-PCN-224(Fe) with peroxidase-like activity and tunable hierarchical porosity was synthesized via a modulator-induced strategy. HP-PCN-224(Fe) not only acts as the enzyme-immobilizat ...
DOI:
10.1021/acsami.8b09388

18. Significant Enhancement of Structural Stability of the Hyperhalophilic ADH from Haloferax volcanii via Entrapment on Metal Organic Framework Support

Author:
Cristina Carucci; Larah Bruen; Victoria Gascón; Francesca Paradisi; Edmond Magner
Source:
Langmuir 2018 v.34 no.28 pp. 8274-8280
ISSN:
1520-5827
Subject:
Haloferax volcanii; acetonitrile; alcohol dehydrogenase; aqueous solutions; catalytic activity; coordination polymers; immobilized enzymes; methanol; pH; solvents; temperature
Abstract:
... The use of an in situ immobilization procedure for the immobilization of hyperhalophilic alcohol dehydrogenase in a metal organic framework material is described. The easy and rapid in situ immobilization process enables retention of activity over a broad range of pH and temperature together with a decrease in the halophilicity of the enzyme. The catalytic activity of the immobilized enzyme was st ...
DOI:
10.1021/acs.langmuir.8b01037

19. Three MOF-Templated Carbon Nanocomposites for Potential Platforms of Enzyme Immobilization with Improved Electrochemical Performance

Author:
Sheying Dong; Lei Peng; Wenbo Wei; Tinglin Huang
Source:
ACS applied materials & interfaces 2018 v.10 no.17 pp. 14665-14672
ISSN:
1944-8252
Subject:
acetylcholinesterase; active sites; annealing; biosensors; carbon; coordination polymers; electrochemistry; electrodes; electron transfer; immobilized enzymes; iron; lanthanum; metal ions; microstructure; nanocomposites; nanomaterials; nitrogen; oxides; parathion-methyl
Abstract:
... An efficient and facile metal–organic framework (MOF)-template strategy for preparing carbon nanocomposites has been developed. First of all, a series of metal ions, including Fe³⁺, Zr⁴⁺, and La³⁺, were respectively connected with 2-aminoterephthalate (H₂ATA) to form three metal-organic frameworks (MOFs) and then three novel MOF-derived materials were obtained by annealing them at 550 °C under N₂ ...
DOI:
10.1021/acsami.8b00702

20. UiO-66-NH2@PMAA: A Hybrid Polymer–MOFs Architecture for Pectinase Immobilization

Author:
Yan Li; Jiangtao Liu; Kehu Zhang; Lei Lei; Zhongli Lei
Source:
Industrial & engineering chemistry process design and development 2018 v.57 no.2 pp. 559-567
ISSN:
1520-5045
Subject:
coordination polymers; desorption; electrostatic interactions; enzyme activity; immobilized enzymes; pH; polygalacturonase; polymerization; polymethylmethacrylate; process design; storage quality
Abstract:
... A hybrid polymer–MOFs architecture UiO-66-NH₂@PMAA was synthesized by tethering polymethacrylic acid (PMAA) onto the surface of the metal–organic frameworks UiO-66-NH₂, and it was further employed for pectinase immobilization by electrostatic interactions. The hybrid architecture was prepared through a combination of atom transfer radical polymerization (ATRP), click chemistry and postsynthetic mo ...
DOI:
10.1021/acs.iecr.7b03398

21. User-Tailored Metal–Organic Frameworks as Supports for Carbonic Anhydrase

Author:
Qian Liu; Jordan Chapman; Aisheng Huang; Kenneth Chandler Williams; Alixandra Wagner; Nagasree Garapati; Konstantinos A. Sierros; Cerasela Zoica Dinu
Source:
ACS applied materials & interfaces 2018 v.10 no.48 pp. 41326-41337
ISSN:
1944-8252
Subject:
adsorption; biomineralization; carbon dioxide; carbonate dehydratase; coordination polymers; crystal structure; desorption; enzyme activity; global warming; heat treatment; hydrophilicity; hydrophobicity; immobilized enzymes; industrial applications; light scattering; models; nitrogen; particle size; scanning electron microscopy; storage quality; surface area; sustainable technology; thermal stability
Abstract:
... Carbonic anhydrase (CA) was previously proposed as a green alternative for biomineralization of carbon dioxide (CO₂). However, enzyme’s fragile nature when in synthetic environment significantly limits such industrial application. Herein, we hypothesized that CA immobilization onto flexible and hydrated “bridges” that ensure proton-transfer at their interfaces leads to improved activity and kineti ...
DOI:
10.1021/acsami.8b14125

22. Adsorption of cholesterol oxidase and entrapment of horseradish peroxidase in metal-organic frameworks for the colorimetric biosensing of cholesterol

Author:
Mengyuan Zhao; Yao Li; Xuejuan Ma; Mengfan Xia; Yaodong Zhang
Source:
Talanta 2019 v.200 pp. 293-299
ISSN:
0039-9140
Subject:
X-ray diffraction; adsorption; biosensors; catalytic activity; cholesterol; colorimetry; confocal microscopy; coordination polymers; encapsulation; enzymatic hydrolysis; immobilized enzymes; pH; peroxidase; porous media; proteinases; scanning electron microscopy; solvents; sorption isotherms; surface area; temperature; thermogravimetry
Abstract:
... The co-immobilization of two enzymes onto single support commonly exhibits low efficiency due to the competition against limited sites. Water-stable metal–organic frameworks (MOFs) [i.e., PCN-333(Al)] with a high surface area and ultra-large cavities were employed to efficiently adsorb cholesterol oxidase (ChOx) and encapsulate horseradish peroxidase (HRP), respectively. The prepared PCN-333/ChOx& ...
DOI:
10.1016/j.talanta.2019.03.060

23. Development of an Immobilized Cellulase System Based on Metal–Organic Frameworks for Improving Ionic Liquid Tolerance and In Situ Saccharification of Bagasse

Author:
Min Zhou; Xin Ju; Zheng Zhou; Lishi Yan; Jiajia Chen; Xuemei Yao; Xinqi Xu; Liang-Zhi Li
Source:
ACS sustainable chemistry & engineering 2019 v.7 no.23 pp. 19185-19193
ISSN:
2168-0485
Subject:
adsorption; bagasse; biomass; cellulose; coordination polymers; dissociation; endo-1,4-beta-glucanase; enzymatic hydrolysis; enzyme activity; hydrolysis; immobilized enzymes; ionic liquids; saccharification
Abstract:
... To achieve high ionic liquid (IL) tolerance and promote in situ enzymatic hydrolysis of biomass, four metal–organic framework (MOF) carriers, including ZIF-8, UIO-66-NH₂, MIL-100-Fe, and PCN-250, were used for cellulase immobilization through simple physical adsorption. The results showed that ZIF-8 had the largest enzyme adsorption capacity at 176.16 mg/g support. In addition, the immobilized cel ...
DOI:
10.1021/acssuschemeng.9b05175

24. Enhancing Enzyme Immobilization on Carbon Nanotubes via Metal–Organic Frameworks for Large-Substrate Biocatalysis

Author:
Sunanda Neupane; Kristen Patnode; Hui Li; Kwaku Baryeh; Guodong Liu; Jinlian Hu; Bingcan Chen; Yanxiong Pan; Zhongyu Yang
Source:
ACS applied materials & interfaces 2019 v.11 no.12 pp. 12133-12141
ISSN:
1944-8252
Subject:
adsorption; amylases; biocatalysis; carbon nanotubes; coordination polymers; cost effectiveness; immobilized enzymes; leaching; lysozyme; models; solvents; synthesis
Abstract:
... Biocatalysis of large-sized substrates finds wide applications. Immobilizing the involved enzymes on solid supports improves biocatalysis yet faces challenges such as enzyme structural perturbation, leaching, and low cost-efficiencies, depending on immobilization strategies/matrices. Carbon nanotubes (CNTs) are attractive matrices but challenged by enzyme leaching (physical adsorption) or perturba ...
DOI:
10.1021/acsami.9b01077

25. Novel bovine carbonic anhydrase encapsulated in a metal–organic framework: a new platform for biomimetic sequestration of CO₂

Author:
Vahideh Asadi; Reihaneh Kardanpour; Shahram Tangestaninejad; Majid Moghadam; Valiollah Mirkhani; Iraj Mohammadpoor-Baltork
Source:
RSC advances 2019 v.9 no.49 pp. 28460-28469
ISSN:
2046-2069
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; adsorption; biocatalysts; biomimetics; calcium carbonate; carbon dioxide; carbon sequestration; carbonate dehydratase; catalytic activity; cattle; coordination polymers; encapsulation; energy-dispersive X-ray analysis; immobilized enzymes; leaching; pH; porous media; scanning electron microscopy; storage quality; temperature; thermal stability; ultraviolet-visible spectroscopy; zeolites
Abstract:
... In this work, maximizing the utilization of CO₂ and its precipitation as CaCO₃ by using immobilized bovine carbonic anhydrase (BCA) was evaluated. In this way, selection of suitable carriers which have a gas adsorption function would enhance the CO₂ sequestration efficiency of the carbonic anhydrase (CA). So a metal–organic framework (MOF), an excellent material for gas adsorption and enzyme immob ...
DOI:
10.1039/c9ra04603h

26. One pot synthesis of α-amylase metal organic framework (MOF)-sponge via dip-coating technique

Author:
Shamraja S. Nadar; Virendra K. Rathod
Source:
International journal of biological macromolecules 2019 v.138 pp. 1035-1043
ISSN:
0141-8130
Subject:
Fourier transform infrared spectroscopy; X-ray diffraction; alpha-amylase; bioactive properties; biotransformation; catalytic activity; coatings; coordination polymers; encapsulation; enzyme kinetics; immobilized enzymes; mass transfer; medicine; melamine; recycling; scanning electron microscopy; starch; surfactants; thermogravimetry
Abstract:
... Enzyme embedded metal–organic frameworks (MOFs) are blooming in the field of enzyme immobilization on account of their superior catalytic efficiency, thermal and chemical stability as compared to native enzyme. However, the separation and recovery of enzyme embedded MOF composite is quite challenging due to its nanometer size, high dispersity and low density which limits the reusability. In this w ...
DOI:
10.1016/j.ijbiomac.2019.07.099

27. Recent advances in metal-organic frameworks for separation and enrichment in proteomics analysis

Author:
Qianjing Liu; Nianrong Sun; Chun-hui Deng
Source:
Trends in analytical chemistry 2019 v.110 pp. 66-80
ISSN:
0165-9936
Subject:
adsorbents; binding sites; chemical species; coordination polymers; immobilized enzymes; ligands; peptides; proteins; proteomics; surface area
Abstract:
... Metal-organic frameworks (MOFs) have been broadly applied to sample preparation which is an essential step in proteomics research. Their large surface area provides abundant binding sites, their specific pore structure can exclude large-sized proteins and allow small molecules to get inside their channels. Besides, the flexible functionalization gives infinite possibilities of MOFs applications in ...
DOI:
10.1016/j.trac.2018.10.033

28. Enzyme Immobilization on Graphite Oxide (GO) Surface via One-Pot Synthesis of GO/Metal–Organic Framework Composites for Large-Substrate Biocatalysis

Author:
Jasmin Farmakes; Isabelle Schuster; Amanda Overby; Lina Alhalhooly; Mary Lenertz; Qiaobin Li; Angel Ugrinov; Yongki Choi; Yanxiong Pan; Zhongyu Yang
Source:
ACS applied materials & interfaces 2020 v.12 no.20 pp. 23119-23126
ISSN:
1944-8252
Subject:
bacteria; biocatalysis; buffers; cell walls; coordination polymers; coprecipitation; electron paramagnetic resonance spectroscopy; graphene; immobilized enzymes; lysozyme; models; pH; synthesis
Abstract:
... Although enzyme immobilization has improved many areas, biocatalysis involving large-size substrates is still challenging for immobilization platform design because of the protein damage under the often “harsh” reaction conditions required for these reactions. Our recent efforts indicate the potential of using Metal–Organic Frameworks (MOFs) to partially confine enzymes on the surface of MOF-based ...
DOI:
10.1021/acsami.0c04101

29. Enzyme embedded metal organic framework (enzyme–MOF): De novo approaches for immobilization

Author:
Shamraja S. Nadar; Leena Vaidya; Virendra K. Rathod
Source:
International journal of biological macromolecules 2020 v.149 pp. 861-876
ISSN:
0141-8130
Subject:
biocomposites; biomineralization; catalytic activity; coordination polymers; coprecipitation; enzyme kinetics; immobilized enzymes; magnetism; porous media; storage quality
Abstract:
... The porous material has been considered as a potential candidate for immobilizing enzymes. Recently, metal organic framework (MOF) has been emerged as a hybrid organic inorganic material with unique intrinsic properties such as well-defined pore structure, excellent chemico-thermal stability, and extremely high surface areas which make them as a suitable scaffold for enzyme immobilization. The out ...
DOI:
10.1016/j.ijbiomac.2020.01.240

30. Enzyme immobilized in BioMOFs: Facile synthesis and improved catalytic performance

Author:
Lihui Wang; Sumin Guan; Jing Bai; Yanjun Jiang; Yang Song; Xiaobing Zheng; Jing Gao
Source:
International journal of biological macromolecules 2020 v.144 pp. 19-28
ISSN:
0141-8130
Subject:
X-ray photoelectron spectroscopy; biocatalysis; biocompatibility; cobalt; coordination polymers; cystine; encapsulation; enzyme activity; immobilized enzymes; ligands; medicine; nicotinamide; nitrile hydratase; pH; recycling; scanning electron microscopy; thermal stability; toxicity; transmission electron microscopy
Abstract:
... Biological metal-organic frameworks (BioMOFs), an emerging sub-class of MOFs, are prepared from metals and biological ligands (bioligands). Benefit from the low toxicity and good biocompatibility of bioligands, BioMOFs can be used in biomedicine and biocatalysis. In this work, a novel approach was developed for fabricating BioMOFs materials (Co-Cys BioMOFs) from cobalt salt and cystine, meanwhile ...
DOI:
10.1016/j.ijbiomac.2019.12.054

31. Hierarchical porous and hydrophilic metal–organic frameworks with enhanced enzyme activity

Author:
Hui Li; Xuedong Lu; Qiuhao Lu; Yi Liu; Xun Cao; Yuanyuan Lu; Xun He; Kequan Chen; Pingkai Ouyang; Weimin Tan
Source:
Chemical communications 2020 v.56 no.34 pp. 4724-4727
ISSN:
1364-548X
Subject:
biocatalysts; biomimetics; catalytic activity; coordination polymers; enzyme activity; hydrophilicity; hydrophobicity; immobilized enzymes; ions; lithocholic acid; mass transfer; mineralization; nanocatalysts; porous media; storage quality; storage time; zinc
Abstract:
... Metal–organic frameworks (MOFs) for enzyme encapsulation-induced biomimetic mineralization under mild reaction conditions are commonly microporous and hydrophobic, which result in a rather high mass transfer resistance of the reactants and restrain the enzyme catalytic activity. Herein, we prepared a type of hierarchical porous and hydrophilic MOF through the biomimetic mineralization of enzymes, ...
DOI:
10.1039/d0cc00748j

32. Immobilized lipase in bio-based metal-organic frameworks constructed by biomimetic mineralization: A sustainable biocatalyst for biodiesel synthesis

Author:
Qing Li; Yingxuan Chen; Shaowei Bai; Xinxin Shao; Lin Jiang; Quanshun Li
Source:
Colloids and surfaces 2020 v.188 pp. 110812
ISSN:
0927-7765
Subject:
Alcaligenes; Fourier transform infrared spectroscopy; X-ray diffraction; adenine; biocatalysts; biodiesel; biomimetics; carboxylic ester hydrolases; catalytic activity; coordination polymers; crystal structure; fuel production; hydrolysis; immobilized enzymes; ligands; metal ions; methanol; mineralization; models; scanning electron microscopy; sunflower oil; temperature; thermogravimetry; transmission electron microscopy; zinc acetate
Abstract:
... Herein, thermophilic lipase QLM from Alcaligenes sp. has been successfully immobilized in bio-based metal-organic frameworks (MOFs) through biomimetic mineralization, using zinc acetate and adenine as metal ion and organic ligand, respectively. The morphology and structure of lipase@Bio-MOF was systematically characterized by scanning electron microcopy (SEM), transmission electron microcopy (TEM) ...
DOI:
10.1016/j.colsurfb.2020.110812

33. Integration of Enzymes and Photosensitizers in a Hierarchical Mesoporous Metal–Organic Framework for Light-Driven CO₂ Reduction

Author:
Yijing Chen; Peng Li; Jiawang Zhou; Cassandra T. Buru; Luka Đorđević; Penghao Li; Xuan Zhang; M. Mustafa Cetin; J. Fraser Stoddart; Samuel I. Stupp; Michael R. Wasielewski; Omar K. Farha
Source:
Journal of the American Chemical Society 2020 v.142 no.4 pp. 1768-1773
ISSN:
1520-5126
Subject:
NAD (coenzyme); carbon dioxide; carbon dioxide fixation; coordination polymers; dicarboxylic acids; electron transfer; encapsulation; formate dehydrogenase; formic acid; immobilized enzymes; irradiation; photosensitizing agents; porous media; renewable energy sources; synthetic products; white light
Abstract:
... Protection of enzymes with synthetic materials is a viable strategy to stabilize, and hence to retain, the reactivity of these highly active biomolecules in non-native environments. Active synthetic supports, coupled to encapsulated enzymes, can enable efficient cascade reactions which are necessary for processes like light-driven CO₂ reduction, providing a promising pathway for alternative energy ...
DOI:
10.1021/jacs.9b12828

34. Metal–Organic Framework in Situ Post-Encapsulating DNA–Enzyme Composites on a Magnetic Carrier with High Stability and Reusability

Author:
Zixin Zhou; Zijing Gao; Hao Shen; Mengqi Li; Wenting He; Ping Su; Jiayi Song; Yi Yang
Source:
ACS applied materials & interfaces 2020 v.12 no.6 pp. 7510-7517
ISSN:
1944-8252
Subject:
DNA; coordination polymers; cross-linking reagents; encapsulation; glucose; immobilized enzymes; magnetism; peroxidase; proteins
Abstract:
... In recent years, metal–organic frameworks (MOFs) have been extensively studied as candidate enzyme immobilization platforms. However, conventional MOF–enzyme composites usually exhibit low controllability and reusability. In this study, a novel and stable strategy for enzyme immobilization was designed by use of ZIF-8 to encapsulate in situ DNA–enzyme composites on the surface of magnetic particle ...
DOI:
10.1021/acsami.9b23526

35. One-pot synthesis and biochemical characterization of protease metal organic framework (protease@MOF) and its application on the hydrolysis of fish protein-waste

Author:
Arastoo Badoei-dalfard; Shima Khankari; Zahra Karami
Source:
Colloids and surfaces 2020 v.196 pp. 111318
ISSN:
0927-7765
Subject:
Bacillus (bacteria); Fourier transform infrared spectroscopy; butanol; calcium; casein; coordination polymers; enzyme activity; fibrin; fish; fluorides; food industry; hydrolysis; immobilized enzymes; pH; storage quality; synthesis; thermal stability; transmission electron microscopy; wastes
Abstract:
... A protease from Bacillus sp. CHA410 was purified and immobilized by a one-step MOF-embedded approach. The immobilized protease characterized using transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). The optimal pH activity of protease CHA410 and protease@MOF was obtained at pH 8.0 and 9.0, respectively. Thermostability re ...
DOI:
10.1016/j.colsurfb.2020.111318

36. A historical overview of the activation and porosity of metal–organic frameworks

Author:
Xuan Zhang; Zhijie Chen; Xinyao Liu; Sylvia L. Hanna; Xingjie Wang; Reza Taheri-Ledari; Ali Maleki; Peng Li; Omar K. Farha
Source:
Chemical Society reviews 2020 v.49 no.20 pp. 7406-7427
ISSN:
1460-4744
Subject:
catalytic activity; coordination polymers; design; drugs; evolution; immobilized enzymes; light; methodology; porosity; porous media; research; separation; society; storage; topology; water
Abstract:
... Since the first reports of metal–organic frameworks (MOFs), this unique class of crystalline, porous materials has garnered increasing attention in a wide variety of applications such as gas storage and separation, catalysis, enzyme immobilization, drug delivery, water capture, and sensing. A fundamental feature of MOFs is their porosity which provides space on the micro- and meso-scale for confin ...
DOI:
10.1039/d0cs00997k

37. Calix[4]arene tetracarboxylic acid-treated lipase immobilized onto metal-organic framework: Biocatalyst for ester hydrolysis and kinetic resolution

Author:
Elif Ozyilmaz; Sebahat Ascioglu; Mustafa Yilmaz
Source:
International journal of biological macromolecules 2021 v.175 pp. 79-86
ISSN:
0141-8130
Subject:
Candida rugosa; aromatic hydrocarbons; biocatalysis; biocatalysts; biocomposites; carboxylic ester hydrolases; coordination polymers; coprecipitation; enantioselectivity; encapsulation; hydrolysis; imidazole; immobilized enzymes; surface area
Abstract:
... Metal organic frameworks (MOFs) are hybrid organic inorganic materials with unique properties such as well-defined pore structure, extremely high surface area, excellent chemical-thermal stability. MOFs-based constructs have been extensively engineered and used for applications, such as enzyme immobilization for bio-catalysis. To obtained a zeolitic imidazole framework-8 (ZIF-8) for enzyme immobil ...
DOI:
10.1016/j.ijbiomac.2021.02.003

38. Encapsulating enzyme into metal-organic framework during in-situ growth on cellulose acetate nanofibers as self-powered glucose biosensor

Author:
Xin Li; Quan Feng; Keyu Lu; Jieyu Huang; Yanan Zhang; Yutong Hou; Hui Qiao; Dawei Li; Qufu Wei
Source:
Biosensors & bioelectronics 2021 v.171 pp. 112690
ISSN:
0956-5663
Subject:
biofuels; biosensors; cellulose acetate; chronic diseases; coordination polymers; disease control; electrodes; glucose; immobilized enzymes; nanofibers; patients
Abstract:
... Development of reliable Enzymatic Biofuel Cells (EBFC)-based self-powered glucose biosensor for continuous, noninvasive monitoring without restriction on patient's movement is highly recommendable. However, its application to a large extent is limited by the relatively poor stability. Herein, we synthesized a highly flexible electrode for effective enzyme immobilization by encapsulating enzyme int ...
DOI:
10.1016/j.bios.2020.112690

39. Immobilization of Lepidium draba peroxidase on a novel Zn-MOF nanostructure

Author:
Soudabeh Farhadi; Ali Riahi-Madvar; Ghasem Sargazi; Mojtaba Mortazavi
Source:
International journal of biological macromolecules 2021 v.173 pp. 366-378
ISSN:
0141-8130
Subject:
Lepidium draba; X-ray diffraction; coordination polymers; fluorescence; immobilized enzymes; pH stability; peroxidase; phosphates; storage quality; temperature; thermal stability; ultrasonic treatment; zinc
Abstract:
... In the present study, ultrasound irradiation was utilized to synthesize a novel zinc metal-organic framework (MOF). Scanning electron microscopic images, exhibited homogenous morphology with a nano-sized distribution of the Zn-MOF structure as also confirmed by X-ray diffraction patterns. Following, physical immobilization of Lepidium draba peroxidase (LDP) were optimized on the Zn-MOF in phosphat ...
DOI:
10.1016/j.ijbiomac.2020.12.216

40. Immobilization of alpha-L-rhamnosidase on a magnetic metal-organic framework to effectively improve its reusability in the hydrolysis of rutin

Author:
Deqing Wang; Pu Zheng; Pengcheng Chen; Dan Wu
Source:
Bioresource technology 2021 v.323 pp. 124611
ISSN:
0960-8524
Subject:
alpha-L-rhamnosidase; biocatalysts; biomass; coordination polymers; crosslinking; hydrolysis; immobilized enzymes; isoquercitrin; magnetism; rutin; solubility
Abstract:
... α-L-Rhamnosidase (Rha) is a biotechnologically important enzyme that degrades biomass containing natural rhamnoside. Herein, the recombinant Rha was successfully immobilized on magnetic metal-organic frameworks (MOFs), and used to hydrolyze rutin. Magnetic MOFs were constructed by binding Cu²⁺ and PABA to the surface of Fe₃O₄ nanoparticles coated with a polydopamine film through coordinate covalen ...
DOI:
10.1016/j.biortech.2020.124611

41. Assembly of peptide linker to amino acid dehydrogenase and immobilized with metal–organic framework

Author:
Shiyan Wang; Lingxuan Duan; Liang Jiang; Kailong Liu; Shizhen Wang
Source:
Journal of chemical technology and biotechnology 2022 v.97 no.3 pp. 741-748
ISSN:
0268-2575
Subject:
amino acids; biotechnology; catalytic activity; coordination polymers; enzyme activity; immobilized enzymes; oxidoreductases; pH; peptides; porous media; surface area
Abstract:
... BACKGROUND: The metal–organic framework (MOF) ZIF‐8 has the advantages of a large specific surface area and high stability. A new strategy for the assembly of amino acid dehydrogenase with peptide linker and for improving enzyme immobilization is proposed. RESULTS: Peptide linker enhanced enzyme activity after immobilization by 138.7%, and maintained 81.2% of its initial activity after seven recyc ...
DOI:
10.1002/jctb.6959

42. Catalase-integrated metal-organic framework with synergetic catalytic activity for colorimetric sensing

Author:
Yanqiu Jing; Jingxin Li; Xuewei Zhang; Mi Sun; Qiang Lei; Bin Li; Jian Yang; Huaiqi Li; Chunguang Li; Xingyou Yang; Liangwen Xie
Source:
Environmental research 2022 v.207 pp. 112147
ISSN:
0013-9351
Subject:
catalase; catalytic activity; colorimetry; coordination polymers; encapsulation; enzyme activity; hydrogen peroxide; immobilized enzymes; phenol; research
Abstract:
... As a platform for enzyme immobilization, metal-organic frameworks (MOFs) can protect enzyme activity from the interference of external adverse environment. Although these strategies have been proven to produce good results, little consideration has been given to the functional similarity of MOFs to the encapsulated enzyme. Here, catalase (CAT) was encapsulated in Fe-BTC with peroxidase-like activi ...
DOI:
10.1016/j.envres.2021.112147

43. Efficient Immobilization of Enzymes on Amino Functionalized MIL-125-NH2 Metal Organic Framework

Author:
Zichen Wang; Yang Liu; Jinhong Li; Guoqing Meng; Daoyu Zhu; Jiandong Cui; Shiru Jia
Source:
Biotechnology and bioprocess engineering 2022 v.27 no.1 pp. 135-144
ISSN:
1226-8372
Subject:
acid tolerance; adsorption; ambient temperature; bioprocessing; biotechnology; catalase; coordination polymers; immobilized enzymes; pH stability; porosity; thermal stability
Abstract:
... As a series of metal organic framework (MOFs), materials of institute lavoisier frameworks (MILs) are expected to be excellent supports for enzyme immobilization due to their good water-stability and acid tolerance. However, enzyme loading on MIL-125-NH₂ is very low due to small pore size and few amino groups on the surface of the MIL-125-NH₂. In this work, catalase (CAT) was immobilized on the MI ...
DOI:
10.1007/s12257-020-0393-y

44. Hydroxyapatite/Glycyrrhizin/Lithium-Based Metal–Organic Framework (HA/GL/Li-MOF) Nanocomposite as Support for Immobilization of Thermomyces lanuginosus Lipase

Author:
Atefeh Ameri; Fahimeh Asadi; Mojtaba Shakibaie; Alieh Ameri; Hamid Forootanfar; Mehdi Ranjbar
Source:
Applied biochemistry and biotechnology 2022 v.194 no.5 pp. 2108-2134
ISSN:
0273-2289
Subject:
Fourier transform infrared spectroscopy; Thermomyces lanuginosus; biotechnology; carboxylic ester hydrolases; coordination polymers; denaturation; energy-dispersive X-ray analysis; enzyme activity; enzyme substrates; experimental design; glutaraldehyde; glycyrrhizin; hot water treatment; hydroxyapatite; immobilized enzymes; lecithins; nanocomposites; pH; thermogravimetry
Abstract:
... The hydroxyapatite/glycyrrhizin/lithium-based metal–organic framework (HA/GL/Li-MOF) nanocomposites were synthesized via the hydrothermal method in the presence of lecithin and glycyrrhizin. Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) equipped with energy-dispersive X-ray spectroscopy (EDS) were applied for characterizati ...
DOI:
10.1007/s12010-022-03800-3

45. Immobilization of D-allulose 3-epimerase into magnetic metal–organic framework nanoparticles for efficient biocatalysis

Author:
Kai Xue; Chun-Li Liu; Yankun Yang; Xiuxia Liu; Jinling Zhan; Zhonghu Bai
Source:
World journal of microbiology & biotechnology 2022 v.38 no.8 pp. 144
ISSN:
0959-3993
Subject:
Agrobacterium radiobacter; biocatalysis; biotechnology; biotransformation; coordination polymers; enzyme activity; fructose; immobilized enzymes; industrial applications; magnetism; mass transfer; microbiology; pH; psicose; temperature; thermal stability
Abstract:
... D-allulose is a rare low-calorie sugar that has many fundamental biological functions. D-allulose 3-epimerase from Agrobacterium tumefaciens (AT-DAEase) catalyzes the conversion of D-fructose to D-allulose. The enzyme has attracted considerable attention because of its mild catalytic properties. However, the bioconversion efficiency and reusability of AT-DAEase limit its industrial application. Ma ...
DOI:
10.1007/s11274-022-03330-4

46. Metal Azolate Coordination Polymer-Enabled High Payload and Non-Destructive Enzyme Immobilization for Biocatalysis and Biosensing

Author:
Hao Zhu; Xiangli Li; Zhimei He; Yun Chen; Jun-Jie Zhu
Source:
Analytical chemistry 2022 v.94 no.18 pp. 6827-6832
ISSN:
1520-6882
Subject:
analytical chemistry; biocatalysis; coordination polymers; cytochrome c; enzyme activity; glucose oxidase; hydrophilicity; immobilized enzymes; pH
Abstract:
... The biomineralized metal–organic frameworks (MOFs) as protective layers help enhance the robustness of enzymes for biocatalysis. Despite great efforts, it is still challenging to develop a recyclable system with high payload and tolerance to harsh conditions. Here, we report a facile surface charge-independent strategy based on Zn-based coordination polymer (ZnCP) for nondestructive immobilization ...
DOI:
10.1021/acs.analchem.2c00637