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- Andreas Baumgartner; Isabel Niederhauser; David Diston; Dominik Moor
- Journal für Verbraucherschutz und Lebensmittelsicherheit 2016 v.11 no.4 pp. 353-357
- Shiga-like toxins; enterohemorrhagic Escherichia coli; food safety; foods; genes; intimin; karsts; quantitative polymerase chain reaction; springs (water); virulence; virulent strains; Switzerland
- ... Within 2 months, two water sources in a karst area in Switzerland were sampled 9 times each, and analyzed by real-time PCR for 6 EHEC O-types, Shiga-like-toxin (stx1 and stx2) and intimin (eae) genes. With the exception of O111, 5 O-types were recorded regularly and at high frequencies (O26: 33.3 %; O157: 33.3 %; O104: 66.6 %; O103: 72.2 %; O145: 94.4 %). Genes for Shiga-like-toxins and intimin we ...
- Stephan, R.; Ragettli, S.; Untermann, F.
- Journal of applied microbiology 2000 v.88 no.2 pp. 335-341
- Shiga toxin-producing Escherichia coli; colicins; enterotoxins; feces; genes; heat stability; human resources; humans; intimin; livestock and meat industry; meat processing plants; plasmids; polymerase chain reaction; serine proteinases; serotypes; virulence; Switzerland
- ... A total of 5590 stool samples from healthy employees in the meat industry were screened by PCR for verotoxin-producing Escherichia coli (VTEC). The PCR product of VT-encoding genes was detected in 3.5% of the samples. Phenotypic and genotypic traits of 47 VTEC strains isolated from asymptomatic carriers were characterized. A variety of serotypes was found; one strain belonged to the serotype O157: ...