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You searched for: Subject isozymes Remove constraint Subject: isozymes Subject chitin Remove constraint Subject: chitin
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1. Efficient conversion of crystalline chitin to N-acetylglucosamine and N,N'-diacetylchitobiose by the enzyme cocktail produced by Paenibacillus sp. LS1

Author:
Saumashish Mukherjee; Prashanta Kumar Behera; Jogi Madhuprakash
Source:
Carbohydrate polymers 2020 v.250 pp. 116889
ISSN:
0144-8617
Subject:
N-acetylglucosamine; Paenibacillus; chitin; chitinase; enzymatic treatment; hydrolysis; isozymes; saccharification; scanning electron microscopy; sodium acetate
Abstract:
... Direct conversion of crystalline chitin to N-acetylglucosamine and the related chitooligomers through enzymatic approaches is gaining importance owing to their various biological applications. Here we report the crystalline chitin degradation ability of chitinolytic cocktail produced by the isolate Paenibacillus sp. LS1. Growth studies of the isolate in presence of different chitin substrates reve ...
DOI:
10.1016/j.carbpol.2020.116889

2. Plant invasion alters the Michaelis–Menten kinetics of microbial extracellular enzymes and soil organic matter chemistry along soil depth

Author:
Kyungjin Min; Vidya Suseela
Source:
Biogeochemistry 2020 v.150 no.2 pp. 181-196
ISSN:
0168-2563
Subject:
Reynoutria japonica; biogeochemistry; chitin; ecological invasion; enzyme activity; enzyme kinetics; fungal biomass; fungi; isozymes; lignin; peroxidase; soil depth; soil organic matter
Abstract:
... Microbial extracellular enzymes decompose distinct components of soil organic matter (SOM), thus influencing its stability. However, we lack the knowledge about how the kinetics of individual enzymes vary when multiple substrates change simultaneously. Here we used Japanese knotweed (Polygonum cuspidatum) invasion as a model system to explore how the Michaelis–Menten kinetics (Vₘₐₓ and kₘ) of micr ...
DOI:
10.1007/s10533-020-00692-5

3. The family of amphioxus chitin synthases offers insight into the evolution of chitin formation in chordates

Author:
Yi Shi; Zhaoyu Fan; Guang Li; Lei Zhang; Zirui Yue; Xinyu Yan; Anlong Xu; Shengfeng Huang
Source:
Molecular phylogenetics and evolution 2020 v.143 pp. 106691
ISSN:
1055-7903
Subject:
Urochordata; ancestry; biopolymers; birds; chitin; eukaryotic cells; fungi; gene pool; gene targeting; genes; genome assembly; hexosyltransferases; invertebrates; isozymes; mammals; tissues
Abstract:
... Chitin is a very important and widely-used biopolymer in fungi and lower metazoans, but mysteriously disappears in mammals. Recent studies reveal that at least lower vertebrates have chitin synthases (CS) and use them to synthesize endogenous chitin. Amphioxus, a basal chordate, therefore becomes critical to understand the evolution of CS, as it occupies the transitional position from invertebrate ...
DOI:
10.1016/j.ympev.2019.106691

4. Antifungal activity and expression patterns of extracellular chitinase and β-1,3-glucanase in Wickerhamomyces anomalus EG2 treated with chitin and glucan

Author:
Sin-Hyoung Hong; Yong-Su Song; Dong-Jun Seo; Kil-Yong Kim; Woo-Jin Jung
Source:
Microbial pathogenesis 2017 v.110 pp. 159-164
ISSN:
0882-4010
Subject:
Fusarium oxysporum; Thanatephorus cucumeris; Wickerhamomyces anomalus; acetazolamide; antifungal properties; beta-glucanase; biological control; biological control agents; caffeine; cell growth; chitin; chitinase; glucans; hyphae; isozymes; kinetin; molecular weight; plant pathogens; polyacrylamide gel electrophoresis; polymers; yeasts
Abstract:
... In this study, the expression patterns of extracellular chitinase and β-1,3-glucanase from cultured Wickerhamomyces anomalus EG2 treated with chitin, glucan, and chemical chitinase inhibitors (kinetin, caffeine, and acetazolamide) were investigated using SDS-PAGE. Relationship between enzyme expression and antifungal activity from yeast plays a very important role for biocontrol of phytopathoges. ...
DOI:
10.1016/j.micpath.2017.06.038

5. Enzyme activity and expression pattern of intra- and extracellular chitinase and β-1,3-glucanase of Wickerhamomyces anomalus EG2 using glycol chitin and glucan-containing high polymer complex

Author:
Sin-Hyoung Hong; Yong-Su Song; Dong-Jun Seo; Kil-Yong Kim; Woo-Jin Jung
Source:
International journal of biological macromolecules 2017 pp. -
ISSN:
0141-8130
Subject:
Wickerhamomyces anomalus; beta-glucanase; cell growth; chitin; chitin deacetylase; chitinase; enzyme activity; isozymes; polyacrylamide gel electrophoresis; polymers
Abstract:
... We investigated cell growth and activity of intra- and extracellular chitinase, β-1,3-glucanase, and chitin deacetylase with SDS‐PAGE by incubating W. anomalus EG2 in PDB and YPD media for 24h in presence of different concentrations (0%, 0.1%, 0.3%, and 0.5%) of colloidal chitin. Maximum cell growth was observed in both PDB and YPD media without colloidal chitin. In the absence of colloidal chitin ...
DOI:
10.1016/j.ijbiomac.2017.07.065

6. Expression and degradation patterns of chitinase purified from Xuehuali (Pyrus bretschneiderilia) pollen

Author:
Yong-Su Song; Sang-Hyun Lee; Jeong-An Cho; Chaeyeong Moon; Dong-Jun Seo; Woo-Jin Jung
Source:
International journal of biological macromolecules 2017 pp. -
ISSN:
0141-8130
Subject:
Pyrus; chitin; chitinase; high performance liquid chromatography; isozymes; oligosaccharides; pH; pollen; polyacrylamide gel electrophoresis; temperature
Abstract:
... The present study investigated the expression pattern of chitinase in Xuehuali (Pyrus bretschneiderilia) pollen, as well as its subsequent degradation. The chitinase was purified and collected using chitin affinity column chromatography with regenerated chitin. After purification, four additional chitinase isozymes (chiA, chiB, chiC, and chiD) and chitinase (Chi II) were clearly expressed on SDS-P ...
DOI:
10.1016/j.ijbiomac.2017.09.010

7. Identification, purification, and expression patterns of chitinase from psychrotolerant Pedobacter sp. PR-M6 and antifungal activity in vitro

Author:
Yong-Su Song; Dong-Jun Seo; Woo-Jin Jung
Source:
Microbial pathogenesis 2017 v.107 pp. 62-68
ISSN:
0882-4010
Subject:
Botrytis cinerea; Pedobacter; Thanatephorus cucumeris; antifungal properties; biological control; chitin; chitinase; chromatography; isozymes; mushrooms; plant pathogens; polyacrylamide gel electrophoresis; soil; soil bacteria; temperature
Abstract:
... In this study, a novel psychrotolerant chitinolytic bacterium Pedobacter sp. PR-M6 that displayed strong chitinolytic activity on 0.5% colloidal chitin was isolated from the soil of a decayed mushroom. Chitinase activity of PR-M6 at 25 °C (C25) after 6 days of incubation with colloidal chitin increased rapidly to a maximum level (31.3 U/mg proteins). Three chitinase isozymes (chiII, chiIII, and ch ...
DOI:
10.1016/j.micpath.2017.03.018

8. Inhibition of IAA oxidase activity of wheat anionic peroxidase by chitooligosaccharides

Author:
I. V. Maksimov; Z. R. Yusupova; E. A. Cherepanova; R. M. Khairulin; V. A. Vakhitov
Source:
Applied biochemistry and microbiology 2016 v.52 no.5 pp. 547-552
ISSN:
0003-6838
Subject:
IAA oxidase; acetylation; chitin; chitooligosaccharides; indole acetic acid; isozymes; molecular weight; oxidation; pH; pathogens; peroxidase; plant tissues; wheat
Abstract:
... The study demonstrated that chitooligosaccharides with a molecular weight of 5–10 kDa and a degree of acetylation of 65% exhibited an auxin-like effect in wheat plants and also played an important role in regulating the activity of polysaccharide (chitin)–specific anion isoenzymes of peroxidase oxidizing indole acetic acid. Changes in the kinetic parameters of the interaction of the wheat anionic ...
DOI:
10.1134/S0003683816050112

9. Enzymatic properties of chitinase-producing antagonistic bacterium Paenibacillus chitinolyticus with various substrates

Author:
Yong-Su Song; Dong-Jun Seo; Wan-Taek Ju; Yong-Seong Lee; Woo-Jin Jung
Source:
Microbial pathogenesis 2015 v.89 pp. 195-200
ISSN:
0882-4010
Subject:
Cicadidae; Paenibacillus; bacteria; chitin; chitinase; crabs; culture media; gels; glucose; isozymes; pH; plant pathogens; polyacrylamide gel electrophoresis; sodium chloride; squid; staining; temperature; yeast extract
Abstract:
... Various chitin substrates were used to investigate the properties of enzymes produced from the chitinase-producing bacterium Paenibacillus chitinolyticus MP-306 against phytopathogens. The MP-306 bacterium was incubated in nine culture media [crab shell powder chitin (CRS), chitin-protein complex powder (CPC), carboxymethyl-chitin powder (CMC), yeast extract only (YE), LB (Trypton, NaCl, and yeast ...
DOI:
10.1016/j.micpath.2015.10.025

10. Characterization of chitinase‐producing Serratia and Bacillus strains isolated from insects

Author:
Kook‐Il Han; Bharat Bhusan Patnaik; Ah‐ra Cho; Ho Kab Lim; Jae Min Lee; Young Geun Jang; Yun Seon Jeong; Tae Kyeong Yoo; Gyeong Seok Lee; Man‐Deuk Han
Source:
Entomological research 2014 v.44 no.3 pp. 109-120
ISSN:
1738-2297
Subject:
Bacillus thuringiensis; Serratia marcescens; Serratia nematodiphila; bacteria; chitin; chitinase; culture media; gels; genes; insect pests; isozymes; plant pathogenic fungi; polyacrylamide gel electrophoresis; ribosomal RNA; saturated fatty acids
Abstract:
... Chitinases (EC 3.2.1.14) are enzymes that hydrolyze chitin by cleaving β‐1,4 N‐glycosidic bonds. These enzymes have been used for multiple applications in biotechnology, especially for controlling insect pests and phytopathogenic fungi. In the present study, we isolated two chitinase‐producing bacteria strains from insects (strain SCH‐1 from Moechotypa diphysis and strain SCH‐2 from Sphedanolestes ...
DOI:
10.1111/1748-5967.12056

11. Expression patterns of chitinase and chitosanase produced from Bacillus cereus in suppression of phytopathogen

Author:
Dong-Jun Seo; Ji-Hae Lee; Yong-Su Song; Ro-Dong Park; Woo-Jin Jung
Source:
Microbial pathogenesis 2014 v.73 pp. 31-36
ISSN:
0882-4010
Subject:
Bacillus cereus; chitin; chitinase; chitosan; chitosanase; culture media; enzyme activity; isozymes; plant pathogens; polyacrylamide gel electrophoresis
Abstract:
... Bacillus cereus MP-310 was incubated on various culture media substrates as LB, colloidal chitin, chitosan powder, and chitosan beads to investigate the concurrent expression patterns of chitinase and chitosanase isozymes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Chitinase activity increased rapidly with a maximum level after 6 days of incubation in CM-chitin medium. ...
DOI:
10.1016/j.micpath.2014.05.007

12. Identification of ecdysteroid signaling late-response genes from different tissues of the Pacific white shrimp, Litopenaeus vannamei

Author:
Zhaoying Qian; Shulin He; Tao Liu; Yongjie Liu; Fujun Hou; Qiao Liu; Xianzong Wang; Xiao Mi; Ping Wang; Xiaolin Liu
Source:
Comparative biochemistry and physiology 2014 v.172 pp. 10-30
ISSN:
1095-6433
Subject:
Litopenaeus vannamei; RNA interference; amino acid sequences; cathepsin L; chitin; chitin synthase; chitinase; double-stranded RNA; ecdysteroid receptor; ecdysteroids; genes; hemocyanin; hepatopancreas; isozymes; metabolism; molting; muscles; myosin heavy chains; quantitative polymerase chain reaction; reproduction; shrimp; tissues
Abstract:
... Ecdysteroids initiate signaling along multiple pathways that regulate various aspects of development, maturation, and reproduction in arthropods. This study was carried out to seek the late target genes of ecdysteroid signaling from different tissues of the Pacific white shrimp, Litopenaeus vannamei. In the present study, eight isoforms of ecdysteroid receptor (EcR), two isoforms of retinoic acid ...
DOI:
10.1016/j.cbpa.2014.02.011

13. Detection of chitinase ChiA produced by Serratia marcescens PRC-5, using anti-PrGV-chitinase

Author:
Yong-Su Song; Seunghan Oh; Yeon-Soo Han; Dong-Jun Seo; Ro-Dong Park; Woo-Jin Jung
Source:
Carbohydrate polymers 2013 v.92 no.2 pp. 2276-2281
ISSN:
0144-8617
Subject:
Serratia marcescens; Thanatephorus cucumeris; agar; bacteria; biological control agents; chitin; chitinase; gels; isozymes; molecular weight; mycelium; pH; polyacrylamide gel electrophoresis; soil
Abstract:
... In this study, a bacterium Serratia marcescens PRC-5 that displayed strong chitinolytic activity on 0.5% colloidal chitin-containing agar medium was isolated from soil. The chitinase activity increased rapidly with a maximum level (6.14U/mL) on 4 days of incubation with swollen chitin (pH 5.0). Three active bands of chitinase isozymes were observed (53, 44, and 34kDa) on SDS-PAGE gel and there pI ...
DOI:
10.1016/j.carbpol.2012.12.018

14. Purification, characterization, and molecular cloning of chitinases from the stomach of the threeline grunt Parapristipoma trilineatum

Author:
Mana Ikeda; Yujiro Kondo; Masahiro Matsumiya
Source:
Process Biochemistry 2013 v.48 pp. 1324-1334
ISSN:
1359-5113
Subject:
Parapristipoma; active sites; chitin; chitinase; complementary DNA; fish; glycosides; isozymes; mammals; molecular cloning; molecular weight; pH; phylogeny; polyacrylamide gel electrophoresis; signal peptide; stomach
Abstract:
... Two chitinase isozymes, PtChiA and PtChiB, were purified from the stomach of the threeline grunt, Parapristipoma trilineatum. The molecular masses of PtChiA and PtChiB were estimated to be 50 and 60kDa by SDS-PAGE, respectively. Both chitinases were stable at pH 3.0–6.0 (acidic) and showed the optimum pH toward both short and long substrates in the acidic region (pH 2.5–5.0). PtChiA and PtChiB pre ...
DOI:
10.1016/j.procbio.2013.06.016

15. Cuticular chitin synthase and chitinase mRNA of whiteleg shrimp Litopenaeus vannamei during the molting cycle

Author:
Jorge Rocha; Fernando L. Garcia-Carreño; Adriana Muhlia-Almazán; Alma B. Peregrino-Uriarte; Gloria Yépiz-Plascencia; Julio H. Córdova-Murueta
Source:
Aquaculture 2012 v.330-333 no. pp. 111-115
ISSN:
0044-8486
Subject:
Litopenaeus vannamei; chitin; chitin synthase; chitinase; complementary DNA; digestion; exoskeleton; foods; genes; gills; hepatopancreas; hydrolysis; integument; isozymes; messenger RNA; metabolism; molting; peritrophic membrane; qualitative analysis; shrimp
Abstract:
... Chitin metabolism is of high relevance for shrimp growth because it has to be synthesized and cleaved in each molt. We studied chitin synthase and chitinase mRNAs from whiteleg shrimp Litopenaeus vannamei. For this, cDNA coding for cuticular chitin synthase (LvChS) and chitinase isoenzymes (LvChi1, LvChi2 and LvChi3) was amplified, sequenced and identified. In a qualitative analysis, LvChi1 and Lv ...
DOI:
10.1016/j.aquaculture.2011.12.024

16. Expression patterns of chitinase produced from Paenibacillus chitinolyticus with different two culture media

Author:
Yong-Su Song; Dong-Jun Seo; Kil-Yong Kim; Ro-Dong Park; Woo-Jin Jung
Source:
Carbohydrate polymers 2012 v.90 no.2 pp. 1187-1192
ISSN:
0144-8617
Subject:
Paenibacillus; chitin; chitinase; culture media; gels; isozymes; metal ions; polyacrylamide gel electrophoresis
Abstract:
... To investigate the expression patterns of chitinase isozymes on native-PAGE and SDS-PAGE gels Paenibacillus chitinolyticus MP-306 was cultured on culture media with and without chitin substrate. P. chitinolyticus MP-306 had a strong chitinolytic activity on colloidal chitin medium. Chitinase isozymes of MP-306 were expressed as six bands (CN1–CN6) on native-PAGE gels and thirteen bands (CS1–CS13) ...
DOI:
10.1016/j.carbpol.2012.06.016

17. Changes in fungal population of fly ash and vinasse mixture during vermicomposting by Eudrilus eugeniae and Eisenia fetida: Documentation of cellulase isozymes in vermicompost

Author:
Prabhat Pramanik; Young Ryun Chung
Source:
Waste management 2011 v.31 no.6 pp. 1169-1175
ISSN:
0956-053X
Subject:
Eisenia fetida; Eudrilus eugeniae; chitin; earthworms; endo-1,4-beta-glucanase; fly ash; fungi; industrial wastes; isozymes; microbial biomass; nutrient content; recycling; vermicomposting; vermicomposts; vinasse
Abstract:
... Fly ash (FA) and vinasse (VN), two industrial wastes, are generated in huge amounts and cause serious hazards to the environment. In this experiment, different proportions of these two wastes were used as food for two epigeic earthworms (Eisenia fetida and Eudrilus eugeniae) to standardize the recycling technique of these two wastes and to study their effect on fungal especially cellulolytic funga ...
DOI:
10.1016/j.wasman.2010.12.017

18. Modification of recombinant maize ChitA chitinase by fungal chitinase-modifying proteins

Author:
Naumann, Todd A.
Source:
Molecular plant pathology 2011 v.12 no.4 pp. 365-372
ISSN:
1464-6722
Subject:
Zea mays; Stenocarpella maydis; genetic resistance; Komagataella pastoris; substrate specificity; aspartic acid; recombinant proteins; proteinases; fungal diseases of plants; Bipolaris; plant pathogenic fungi; fungal proteins; disease resistance; mutants; binding sites; isozymes; genetic polymorphism; chitin; chitinase
Abstract:
... In commercial maize, there are at least two different alleles of the chiA gene that encode alloforms of ChitA chitinase, a protein that is abundant in developing seed. Both known alloforms are modified by Bz-cmp, a chitinase-modifying protein (cmp) secreted by the fungal pathogen Bipolaris zeicola. One alloform (ChitA-B73) is also modified by Stm-cmp, a protein secreted by the fungal pathogen Sten ...
Handle:
10113/49585
DOI:
10.1111/j.1364-3703.2010.00677.x
PubMed:
21453431
PubMed Central:
PMC6640348

19. Effects of carbon and nitrogen sources on the induction and repression of chitinase enzyme from Metarhizium anisopliae isolates

Author:
Dhar, Priyanka; Kaur, Gurvinder
Source:
Annals of microbiology 2009 v.59 no.3 pp. 545-551
ISSN:
1590-4261
Subject:
Metarhizium anisopliae; chitinase; enzyme activity; biosynthesis; nutrient availability; chitin; glucose; nitrogen; yeast extract; isozymes; biological control agents; entomopathogenic fungi
DOI:
10.1007/BF03175144

20. Purification and characterization of chitinases from Paecilomyces variotii DG-3 parasitizing on Meloidogyne incognita eggs

Author:
Nguyen, Van-Nam; Oh, In-Jae; Kim, Young-Ju; Kim, Kil-Yong; Kim, Young-Cheol; Park, Ro-Dong
Source:
Journal of industrial microbiology & biotechnology 2009 v.36 no.2 pp. 195-203
ISSN:
1367-5435
Subject:
Paecilomyces; soil fungi; animal pathogenic fungi; nematophagous fungi; biological control agents; chitinase; isozymes; enzyme activity; enzyme substrates; chitin; oligosaccharides; enzymatic hydrolysis; substrate specificity; sequence analysis; Meloidogyne incognita; plant parasitic nematodes; nematode control; biological control; cucumbers; Cucumis sativus; amino acid sequences; South Korea
Abstract:
... Two extracellular chitinases were purified from Paecilomyces variotii DG-3, a chitinase producer and a nematode egg-parasitic fungus, to homogeneity by DEAE Sephadex A-50 and Sephadex G-100 chromatography. The purified enzymes were a monomer with an apparent molecular mass of 32 kDa (Chi32) and 46 kDa (Chi46), respectively, and showed chitinase activity bands with 0.01% glycol chitin as a substrat ...
DOI:
10.1007/s10295-008-0485-8
PubMed:
18936994