Subject: medicine and genetic engineering / Subject term: medicine and genetic engineering

1. Direct Cell Radiolabeling for in Vivo Cell Tracking with PET and SPECT Imaging

Author:: Peter J. Gawne; Francis Man; Philip J. Blower; Rafael T. M. de Rosales
Source:: Chemical reviews 2022 v.122 no.11 pp. 10266-10318
ISSN:: 1520-6890
Subject:: biochemistry; chemistry; genetic engineering; medicine; photons; positron-emission tomography; radio; radiolabeling; therapeutics
Abstract:: ... The arrival of cell-based therapies is a revolution in medicine. However, its safe clinical application in a rational manner depends on reliable, clinically applicable methods for determining the fate and trafficking of therapeutic cells in vivo using medical imaging techniques─known as in vivo cell tracking. Radionuclide imaging using single photon emission computed tomography (SPECT) or positron ...
DOI:: 10.1021/acs.chemrev.1c00767
: https://doi.org/10.1021/acs.chemrev.1c00767

2. Biotechnological applications of Bacillus licheniformis

Author:: Andrea Muras; Manuel Romero; Celia Mayer; Ana Otero
Source:: Critical reviews in biotechnology 2021 v.41 no.4 pp. 609-627
ISSN:: 1549-7801
Subject:: Bacillus licheniformis; antibiotic resistance; antimicrobial properties; aquaculture; bacteria; biobased products; biomineralization; bioremediation; biotechnology; feed additives; fuel production; genetic engineering; genetic vectors; medicine; probiotics
Abstract:: ... Bacillus licheniformis is a Gram positive spore-forming bacterial species of high biotechnological interest with numerous present and potential uses, including the production of bioactive compounds that are applied in a wide range of fields, such as aquaculture, agriculture, food, biomedicine, and pharmaceutical industries. Its use as an expression vector for the production of enzymes and other bi ...
DOI:: 10.1080/07388551.2021.1873239
: https://doi.org/10.1080/07388551.2021.1873239

3. The role of genetic manipulation and in situ modifications on production of bacterial nanocellulose: A review

Author:: Mehran Moradi; Paulina Jacek; Azra Farhangfar; Jonas T. Guimarães; Mehrdad Forough
Source:: International journal of biological macromolecules 2021 v.183 pp. 635-650
ISSN:: 0141-8130
Subject:: biocompatible materials; biopolymers; bioprocessing; cellulose; coculture; genetic engineering; medicine; pharmacology; porosity
Abstract:: ... Natural polysaccharides are well-known biomaterials because of their availability and low-cost, with applications in diverse fields. Cellulose, a renowned polysaccharide, can be obtained from different sources including plants, algae, and bacteria, but recently much attention has been paid to the microorganisms due to their potential of producing renewable compounds. In this regard, bacterial nano ...
DOI:: 10.1016/j.ijbiomac.2021.04.173
: https://doi.org/10.1016/j.ijbiomac.2021.04.173

4. Transduction Enhancers Enable Efficient Human Adenovirus Type 5-Mediated Gene Transfer into Human Multipotent Mesenchymal Stromal Cells

Author:: Robin Nilson; Olivia Lübbers; Linus Weiß; Karmveer Singh; Karin Scharffetter-Kochanek; Markus Rojewski; Hubert Schrezenmeier; Philip Helge Zeplin; Wolfgang Funk; Lea Krutzke; Stefan Kochanek; Astrid Kritzinger
Source:: Viruses 2021 v.13 no.6 pp. -
ISSN:: 1999-4915
Subject:: Human mastadenovirus C; blood coagulation; cancer therapy; capsid; gene transfer; genes; genetic engineering; humans; immunomodulation; lactoferrin; medicine; migratory behavior; spermidine; spermine; tumor necrosis factors
Abstract:: ... Human multipotent mesenchymal stromal cells (hMSCs) are currently developed as cell therapeutics for different applications, including regenerative medicine, immune modulation, and cancer treatment. The biological properties of hMSCs can be further modulated by genetic engineering. Viral vectors based on human adenovirus type 5 (HAdV-5) belong to the most frequently used vector types for genetic m ...
DOI:: 10.3390/v13061136
: https://doi.org/10.3390/v13061136

5. Biotechnology of camptothecin production in Nothapodytes nimmoniana, Ophiorrhiza sp. and Camptotheca acuminata

Author:: Mallappa Kumara Swamy; Shreya Nath; Subhabrata Paul; Niraj Kumar Jha; Boregowda Purushotham; Komdur Channabasavaraju Rohit; Abhijit Dey
Source:: Applied microbiology and biotechnology 2021 v.105 no.24 pp. 9089-9102
ISSN:: 0175-7598
Subject:: Camptotheca acuminata; Icacinaceae; Ophiorrhiza; antineoplastic agents; bark; biosynthesis; biotechnology; cancer therapy; drug development; endangered species; ex situ conservation; genetic engineering; genetic variation; habitats; human diseases; humans; medicinal plants; medicine; microbiology; plant biochemistry; secondary metabolites; traditional farming
Abstract:: ... Cancer is probably the deadliest human disease in recent years. In the past few years, rapid clinical progress has been made in the field of anticancer drug development. Plant secondary metabolites have been noted as extremely efficacious as promising natural source for anticancer therapy for many years. Camptothecin (CPT) is one of the popularly used anti-tumor drugs possessing clinically proven ...
DOI:: 10.1007/s00253-021-11700-5
: https://doi.org/10.1007/s00253-021-11700-5

6. Breeding of a cordycepin-resistant and adenosine kinase-deficient sake yeast strain that accumulates high levels of S-adenosylmethionine

Author:: Muneyoshi Kanai; Norito Yasuda; Tomoko Morimoto; Satoko Yoshida; Nahoko Nishibori; Masaki Mizunuma; Tsutomu Fujii; Haruyuki Iefuji
Source:: Bioscience, biotechnology, and biochemistry 2019 v.83 no.8 pp. 1530-1537
ISSN:: 1347-6947
Subject:: S-adenosylmethionine; adenosine kinase; biotechnology; cordycepin; evolution; genetic engineering; industry; medicine; mutation; sake; yeasts
Abstract:: ... Adenosine kinase (ADO1)-deficient mutants can be obtained from cordycepin-resistant strains, and the disruption of ADO1 causes S-adenosylmethionine (SAM) accumulation. To breed a high-SAM-accumulating yeast strain without genetic manipulation for industrial purposes, we bred a cordycepin-resistant strain using sake yeast kyokai No. 9 as the parent strain with a mutation in adenosine kinase (ADO1) ...
DOI:: 10.1080/09168451.2019.1571896
: https://doi.org/10.1080/09168451.2019.1571896

7. Calcium carbonate supplementation to chorioallantoic membranes improves hatchability in shell-less chick embryo culture

Author:: Yutaka Tahara; Katsuya Obara; Masamichi Kamihira
Source:: Journal of bioscience and bioengineering 2021 v.131 no.3 pp. 314-319
ISSN:: 1389-1723
Subject:: calcium; calcium carbonate; carcinogenesis; chick embryos; chicks; chorioallantoic membrane; embryo culture; embryogenesis; genetic engineering; medicine; plastic film; toxicology
Abstract:: ... Developing chick embryos are a classical research tool in developmental biology. The whole embryo culture technique can be applied to various fields, such as embryo manipulation, toxicology, tumorigenesis, and basic research in regenerative medicine. When used for the generation of transgenic chickens, a high hatchability of genetically engineered embryos is essential to support normal embryonic d ...
DOI:: 10.1016/j.jbiosc.2020.11.001
: https://doi.org/10.1016/j.jbiosc.2020.11.001

8. Current strategies in tailoring methods for engineered exosomes and future avenues in biomedical applications

Author:: Ankita Mishra; Prerna Singh; Irfan Qayoom; Abhay Prasad; Ashok Kumar
Source:: Journal of materials chemistry B 2021 v.9 no.32 pp. 6281-6309
ISSN:: 2050-7518
Subject:: chemistry; drugs; exosomes; genetic engineering; medicine; therapeutics
Abstract:: ... Exosomes are naturally occurring nanovesicles of endosomal origin, responsible for cellular communication. Depending on the cell type, exosomes display disparity in the cargo and are involved in up/down regulation of different biological pathways. Naturally secreted exosomes, owing to their inherent delivery potential, non-immunogenic nature and limited structural resemblance to the cells have eme ...
DOI:: 10.1039/d1tb01088c
: https://doi.org/10.1039/d1tb01088c

9. De novo assembly and comparative transcriptome analysis: novel insights into terpenoid biosynthesis in Chamaemelum nobile L.

Author:: Xiaomeng Liu; Xiaohui Wang; Zexiong Chen; Jiabao Ye; Yongling Liao; Weiwei Zhang; Jie Chang; Feng Xu
Source:: Plant cell reports 2019 v.38 no.1 pp. 101-116
ISSN:: 0721-7714
Subject:: Chamaemelum nobile; alpha-pinene; alpha-terpineol; beta-phellandrene; biosynthesis; camphor; carvacrol; chemical composition; cineole; computer software; cosmetics; essential oils; flowers; gas chromatography-mass spectrometry; gene expression regulation; genetic engineering; herbal medicines; high-throughput nucleotide sequencing; leaves; medicinal plants; medicine; messenger RNA; quantitative polymerase chain reaction; roots; stems; transcriptome; transcriptomics; unigenes
Abstract:: ... KEY MESSAGE: Analysis of terpenoids content, transcriptome from Chamaemelum nobile showed that the content of the terpenoids in the roots was the highest and key genes involved in the terpenoids synthesis pathway were identified. Chamaemelum nobile is a widely used herbaceous medicinal plant rich in volatile oils, mainly composed of terpenoids. It is widely used in food, cosmetics, medicine, and o ...
DOI:: 10.1007/s00299-018-2352-z
: https://doi.org/10.1007/s00299-018-2352-z

10. Efficient plant regeneration and histological evaluations of regenerants through organogenesis and somatic embryogenesis in Spermacoce hispida L.—An underutilized medicinally important plant

Author:: K.V. Deepak; J. Johnny subakar ivin; G. Sathiya Narayanan; M. Prakash; S. Murugan; R. Anandan
Source:: Industrial crops and products 2019 v.134 pp. 292-302
ISSN:: 0926-6690
Subject:: 2,4-D; Spermacoce; benzyladenine; callus; developmental stages; explants; genetic engineering; germination; germplasm conservation; habitats; histology; hypocotyls; kinetin; leaves; medicine; organogenesis; phenotype; plantlets; protocols; shoot tips; somatic embryogenesis; somatic embryos; tissue culture; weeds
Abstract:: ... Shaggy button weed (Spermacoce hispida L.) is a therapeutically important herb widely used in Indian System of medicine particularly Ayurvedic, Siddha, and Unani. S. hispida is recalcitrant during germination in natural habitat. Plant tissue culture is an alternate approach for mass multiplication of such vulnerable plants. Plant regeneration by organogenesis and somatic embryogenesis in S. hispid ...
DOI:: 10.1016/j.indcrop.2019.03.067
: https://doi.org/10.1016/j.indcrop.2019.03.067

11. High Aspect Ratio and Light-Sensitive Micropillars Based on a Semiconducting Polymer Optically Regulate Neuronal Growth

Author:: Frano Milos; Gabriele Tullii; Federico Gobbo; Francesco Lodola; Francesco Galeotti; Chiara Verpelli; Dirk Mayer; Vanessa Maybeck; Andreas Offenhäusser; Maria Rosa Antognazza
Source:: ACS applied materials & interfaces 2021 v.13 no.20 pp. 23438-23451
ISSN:: 1944-8252
Subject:: cell growth; genetic engineering; light; medicine; microrelief; neurites; photons; polymers; prototypes; semiconductors
Abstract:: ... Many nano- and microstructured devices capable of promoting neuronal growth and network formation have been previously investigated. In certain cases, topographical cues have been successfully complemented with external bias, by employing electrically conducting scaffolds. However, the use of optical stimulation with topographical cues was rarely addressed in this context, and the development of l ...
DOI:: 10.1021/acsami.1c03537
: https://doi.org/10.1021/acsami.1c03537

12. Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Author:: Christoph Wawrosch; Sergey B. Zotchev
Source:: Applied microbiology and biotechnology 2021 v.105 no.18 pp. 6649-6668
ISSN:: 0175-7598
Subject:: biochemical pathways; biotechnology; commercialization; cost effectiveness; genetic engineering; genetically modified organisms; humans; indigenous species; medicine; phytochemicals; raw materials; secondary metabolites; synthesis; tissue culture
Abstract:: ... Medicinal plants have been used by mankind since ancient times, and many bioactive plant secondary metabolites are applied nowadays both directly as drugs, and as raw materials for semi-synthetic modifications. However, the structural complexity often thwarts cost-efficient chemical synthesis, and the usually low content in the native plant necessitates the processing of large amounts of field-cul ...
DOI:: 10.1007/s00253-021-11539-w
: https://doi.org/10.1007/s00253-021-11539-w

13. Research advances on arachidonic acid production by fermentation and genetic modification of Mortierella alpina

Author:: Huidan Zhang; Qiu Cui; Xiaojin Song
Source:: World journal of microbiology & biotechnology 2021 v.37 no.1 pp. 4
ISSN:: 0959-3993
Subject:: Mortierella alpina; arachidonic acid; biotechnology; cosmetics; culture media; fermentation; genetic engineering; humans; medicine; nutrition; synthetic biology
Abstract:: ... Arachidonic acid (ARA, 5, 8, 11, 14-cis-eicosatetraenoic acid) is a relevant ω-6 polyunsaturated fatty acid, which plays essential roles in human immune, cardiovascular, and nervous systems. It is widely used in medicine, cosmetics, nutrition, and other fields. Traditionally, ARA is obtained from animal tissues. However, due to the limitation and unsustainability of existing resources, microorgani ...
DOI:: 10.1007/s11274-020-02984-2
: https://doi.org/10.1007/s11274-020-02984-2

14. A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology

Author:: Arturo Casini; Fang-Yuan Chang; Raissa Eluere; Andrew M. King; Eric M. Young; Quentin M. Dudley; Ashty Karim; Katelin Pratt; Cassandra Bristol; Anthony Forget; Amar Ghodasara; Robert Warden-Rothman; Rui Gan; Alexander Cristofaro; Amin Espah Borujeni; Min-Hyung Ryu; Jian Li; Yong-Chan Kwon; He Wang; Evangelos Tatsis; Carlos Rodriguez-Lopez; Sarah O’Connor; Marnix H. Medema; Michael A. Fischbach; Michael C. Jewett; Christopher Voigt; D. Benjamin Gordon
Source:: Journal of the American Chemical Society 2018 v.140 no.12 pp. 4302-4316
ISSN:: 1520-5126
Subject:: DNA; Escherichia coli; Saccharomyces cerevisiae; Streptomyces albidoflavus; Streptomyces albovinaceus; Streptomyces coelicolor; anti-infective agents; biosynthesis; cell free system; genetic engineering; industry; medicine; monoterpenoids; tetrahydrofuran; toxicity; vincristine
Abstract:: ... Centralized facilities for genetic engineering, or “biofoundries”, offer the potential to design organisms to address emerging needs in medicine, agriculture, industry, and defense. The field has seen rapid advances in technology, but it is difficult to gauge current capabilities or identify gaps across projects. To this end, our foundry was assessed via a timed “pressure test”, in which 3 months ...
DOI:: 10.1021/jacs.7b13292
: https://doi.org/10.1021/jacs.7b13292

15. Bacteria and cancer: Different sides of the same coin

Author:: Ghazaleh Laliani; Shokufeh Ghasemian Sorboni; Roya Lari; Atieh Yaghoubi; Saman Soleimanpour; Majid Khazaei; Seyed Mahdi Hasanian; Amir Avan
Source:: Life sciences 2020 v.246 pp. 117398
ISSN:: 0024-3205
Subject:: bacteria; cancer therapy; cell death; drug therapy; genetic engineering; immunotherapy; medicine; metabolites; neoplasm cells; neoplasms; radiotherapy; recombinant vaccines
Abstract:: ... Conventional cancer therapies such as chemotherapy, radiation therapy, and immunotherapy due to the complexity of cancer have been unsuccessful in the complete eradication of tumor cells. Thus, there is a need for new therapeutic strategies toward cancer. Recently, the therapeutic role of bacteria in different fields of medicine and pharmaceutical research has attracted attention in recent decades ...
DOI:: 10.1016/j.lfs.2020.117398
: https://doi.org/10.1016/j.lfs.2020.117398

16. Genetically Engineered Adipose Mesenchymal Stem Cells Using HIV-Based Lentiviral Vectors as Gene Therapy for Autoimmune Diseases

Author:: Masoumeh Rostami; Kamran Haidari; Majid Shahbazi
Source:: Cellular reprogramming 2018 v.20 no.6 pp. 337-346
ISSN:: 2152-4998
Subject:: CD4-positive T-lymphocytes; autoimmune diseases; bioactive properties; gene therapy; genetic engineering; humans; immunosuppression; inflammation; interleukin-10; interleukin-17; interleukin-23; medicine; mesenchymal stromal cells; mice; quantitative polymerase chain reaction; splenocytes; transcription factors
Abstract:: ... The immunomodulatory and self-renewable features of human adipose-derived mesenchymal stem cells (hAD-MSCs) mark their importance in regenerative medicine. Interleukin (IL)-23 as a proinflammatory cytokine suppresses T regulatory cells and promotes the response of T helper 17 and T helper 1 cells. This pathway initiates inflammation and immunosuppression in several autoimmune diseases. The current ...
DOI:: 10.1089/cell.2018.0006
: https://doi.org/10.1089/cell.2018.0006

17. Individual identification and marking techniques for zebrafish

Author:: Johann Delcourt; Michaël Ovidio; Mathieu Denoël; Marc Muller; Hélène Pendeville; Jean-Louis Deneubourg; Pascal Poncin
Source:: Reviews in fish biology and fisheries 2018 v.28 no.4 pp. 839-864
ISSN:: 0960-3166
Subject:: Danio rerio; animal identification; aquaculture; color; durability; ecology; ecotoxicology; ethics; fish; genetic engineering; image analysis; managers; medicine; microchip technology; models; neurophysiology; researchers; transponders
Abstract:: ... In laboratory fish research, the zebrafish Danio rerio (Cyprinidae) represents the equivalent of the mouse in mammalian research. This species has become a major model for studies in developmental and behavioural genetics, neurophysiology, biomedicine, ecotoxicology, and behavioural and evolutionary ecology. To meet the need for accurate and reproducible data in both fundamental and applied scienc ...
DOI:: 10.1007/s11160-018-9537-y
: https://doi.org/10.1007/s11160-018-9537-y

18. Innovations in CRISPR technology

Author:: Alexandra K Brooks; Thomas Gaj
Source:: Current opinion in biotechnology 2018 v.52 pp. 95-101
ISSN:: 0958-1669
Subject:: biotechnology; gene therapy; genetic engineering; medicine
Abstract:: ... CRISPR-Cas9 is a versatile tool for genome engineering that has revolutionized biotechnology and is poised to impact medicine. Recent advances in the identification of unique CRISPR systems, as well as the re-engineering of the Cas9 protein for expanded function, has enabled the diversification of the CRISPR genome engineering toolbox. In this review, we highlight these innovations and discuss how ...
DOI:: 10.1016/j.copbio.2018.03.007
: https://doi.org/10.1016/j.copbio.2018.03.007

19. Optogenetics sheds new light on tissue engineering and regenerative medicine

Author:: Wenzhi Hu; Qiankun Li; Bingmin Li; Kui Ma; Cuiping Zhang; Xiaobing Fu
Source:: Biomaterials 2020 v.227 pp. 119546
ISSN:: 0142-9612
Subject:: biocompatible materials; embryogenesis; genetic engineering; medicine; optogenetics; personal development; signal transduction; tissue engineering
Abstract:: ... Optogenetics has demonstrated great potential in the fields of tissue engineering and regenerative medicine, from basic research to clinical applications. Spatiotemporal encoding during individual development has been widely identified and is considered a novel strategy for regeneration. A as a noninvasive method with high spatiotemporal resolution, optogenetics are suitable for this strategy. In ...
DOI:: 10.1016/j.biomaterials.2019.119546
: https://doi.org/10.1016/j.biomaterials.2019.119546

20. Polyhydroxybutyrate production from marine source and its application

Author:: Ganapathy Kavitha; Ramasamy Rengasamy; Dhinakarasamy Inbakandan
Source:: International journal of biological macromolecules 2018 v.111 pp. 102-108
ISSN:: 0141-8130
Subject:: Actinobacteria; Cyanobacteria; aquaculture; bacteria; biodegradability; bioplastics; genetic engineering; macroalgae; medicine; microalgae; polyhydroxybutyrate; thermoplastics; tissue engineering; wastes
Abstract:: ... The increasing significance of non-degradable plastic wastes is an emerging concern. As a substitute, researches are being endeavoured from existing reserve to yield bioplastics based on their properties of biodegradability. Owing to their cost, now the experts are quest for a substitute source like bacteria, microalgae, actinomycetes, cyanobacteria and plants. PHB is biodegradable, environmental ...
DOI:: 10.1016/j.ijbiomac.2017.12.155
: https://doi.org/10.1016/j.ijbiomac.2017.12.155

21. 182 Establishment of expanded potential embryonic stem cell lines from porcine embryos

Author:: M. Nowak-Imialek; X. Gao; P. Liu; H. Niemann
Source:: Reproduction, fertility, and development 2019 v.31 no.1 pp. 215-216
ISSN:: 1031-3613
Subject:: GATA transcription factors; cell culture; cell lines; embryonic germ layers; embryonic stem cells; fetus; flow cytometry; genetic engineering; germ cells; karyotyping; medicine; mice; parthenogenesis; reverse transcriptase polymerase chain reaction; sows; tissues; transgenes; trophoblast; tubulin
Abstract:: ... The domestic pig is an excellent large animal in biomedical medicine and holds great potential for testing the clinical safety and efficacy of stem cell therapies. Previously, numerous studies reported the derivation of porcine embryonic stem cell (ESC)-like lines, but none of these lines fulfilled the stringent criteria for true pluripotent germline competent ESC. Here, we report the first establ ...
DOI:: 10.1071/RDv31n1Ab182
: https://doi.org/10.1071/RDv31n1Ab182

22. An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells

Author:: Krishna Kumar Haridhasapavalan; Manash P. Borgohain; Chandrima Dey; Bitan Saha; Gloria Narayan; Sachin Kumar; Rajkumar P. Thummer
Source:: Gene 2019 v.686 pp. 146-159
ISSN:: 0378-1119
Subject:: induced pluripotent stem cells; risk; Murine respirovirus; medicine; humans; drug evaluation; ethics; somatic cells; plasmids; therapeutics; transfection; embryonic stem cells; genetic engineering; genomics; fibroblasts; Adenoviridae; transposons
Abstract:: ... Over a decade ago, a landmark study that reported derivation of induced Pluripotent Stem Cells (iPSCs) by reprogramming fibroblasts has transformed stem cell research attracting the interest of the scientific community worldwide. These cells circumvent the ethical and immunological concerns associated with embryonic stem cells, and the limited self-renewal ability and restricted differentiation po ...
DOI:: 10.1016/j.gene.2018.11.069
: https://doi.org/10.1016/j.gene.2018.11.069

23. Bacillus thuringiensis: a successful insecticide with new environmental features and tidings

Author:: Gholamreza Salehi Jouzani; Elena Valijanian; Reza Sharafi
Source:: Applied microbiology and biotechnology 2017 v.101 no.7 pp. 2691-2711
ISSN:: 0175-7598
Subject:: Bacillus thuringiensis; Nematoda; animal pathogenic bacteria; antineoplastic activity; biobased products; bioremediation; biosynthesis; fungi; genes; genetic engineering; genetically modified plants; heavy metals; insect pests; insecticides; medicine; mites; molecular biology; nanoparticles; plant growth-promoting rhizobacteria; pollutants; polyhydroxyalkanoates; ticks; toxicity; toxins
Abstract:: ... Bacillus thuringiensis (Bt) is known as the most successful microbial insecticide against different orders of insect pests in agriculture and medicine. Moreover, Bt toxin genes also have been efficiently used to enhance resistance to insect pests in genetically modified crops. In light of the scientific advantages of new molecular biology technologies, recently, some other new potentials of Bt hav ...
DOI:: 10.1007/s00253-017-8175-y
: https://doi.org/10.1007/s00253-017-8175-y

24. Bacteriophages immobilized on electrospun cellulose microfibers by non-specific adsorption, protein–ligand binding, and electrostatic interactions

Author:: Erica Vonasek; Ping Lu; You-Lo Hsieh; Nitin Nitin
Source:: Cellulose 2017 v.24 no.10 pp. 4581-4589
ISSN:: 0969-0239
Subject:: adsorption; bacteriophages; biocompatible materials; cellulose; electrostatic interactions; food pathogens; genetic engineering; image analysis; medicine; phage therapy
Abstract:: ... Phage therapy has significant potential in specifically targeting bacterial pathogens in food and medicine. There is a significant interest to combine phages with materials to enhance and broaden potential applications of phages. This study compares non-specific adsorption, protein–ligand binding, and electrostatic interactions on cellulose microfibers without any chemical or genetic modification ...
DOI:: 10.1007/s10570-017-1442-3
CHORUS:: 10.1007/s10570-017-1442-3
: https://doi.org/10.1007/s10570-017-1442-3

25. Extremophilic exopolysaccharides: A review and new perspectives on engineering strategies and applications

Author:: Jia Wang; David R. Salem; Rajesh K. Sani
Source:: Carbohydrate polymers 2019 v.205 pp. 8-26
ISSN:: 0144-8617
Subject:: biocompatible materials; biopolymers; engineering; exopolysaccharides; extremophiles; food industry; genetic engineering; industrialization; medicine; microorganisms; niches
Abstract:: ... Numerous microorganisms inhabiting harsh niches produce exopolysaccharides as a significant strategy to survive in extreme conditions. The exopolysaccharides synthesized by extremophiles possess distinctive characteristics due to the varied harsh environments which stimulate the microorganisms to produce these biopolymers. Despite many bioprocesses have been designed to yield exopolysaccharides, t ...
DOI:: 10.1016/j.carbpol.2018.10.011
: https://doi.org/10.1016/j.carbpol.2018.10.011

26. Genetic circuitry for personalized human cell therapy

Author:: Fabian Tolle; Pascal Stücheli; Martin Fussenegger
Source:: Current opinion in biotechnology 2019 v.59 pp. 31-38
ISSN:: 0958-1669
Subject:: gene expression; gene regulatory networks; genetic engineering; humans; medical treatment; medicine; protein synthesis; synthetic biology; synthetic genes
Abstract:: ... Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for precise spatiotemporal control of target gene expression in living cells. Designer cel ...
DOI:: 10.1016/j.copbio.2019.02.003
: https://doi.org/10.1016/j.copbio.2019.02.003

27. Genetic platforms for heterologous expression of microbial natural products

Author:: Jia Jia Zhang; Xiaoyu Tang; Bradley S. Moore
Source:: Natural product reports 2019 v.36 no.9 pp. 1313-1332
ISSN:: 1460-4752
Subject:: DNA replication; biosynthesis; genetic engineering; heterologous gene expression; immunology; medicine; multigene family
Abstract:: ... Covering: 2005 up to 2019 Natural products are of paramount importance in human medicine. Not only are most antibacterial and anticancer drugs derived directly from or inspired by natural products, many other branches of medicine, such as immunology, neurology, and cardiology, have similarly benefited from natural product-based drugs. Typically, the genetic material required to synthesize a microb ...
DOI:: 10.1039/c9np00025a
PubMed:: 31197291
PubMed Central:: PMC6750982
: https://doi.org/10.1039/c9np00025a

28. Purification and characterization of a cystatin like thiol protease inhibitor from Brassica nigra

Author:: Anna Feroz; Peerzada Shariq Shaheen Khaki; Azad Alam Siddiqui; Fakhra Amin; Mohd Sajid Khan; Bilqees Bano
Source:: International journal of biological macromolecules 2019 v.125 pp. 1128-1139
ISSN:: 0141-8130
Subject:: Brassica nigra; Fourier transform infrared spectroscopy; ammonium sulfate; bromelains; calorimetry; circular dichroism spectroscopy; cystatins; diffusivity; ficain; fluorescence; food technology; fractionation; gel chromatography; genetic engineering; kinetics; medicine; molecular weight; papain; polyacrylamide gel electrophoresis; protein metabolism; proteins; seeds; spectral analysis; stoichiometry; thermodynamics; titration
Abstract:: ... Phytocystatins or plant cystatins belong to a group of thiol protease inhibitors present ubiquitously in living system. They play a crucial role in cellular protein turnover thereby showing involvement in a wide array of physiological processes in plants. With wide importance and tremendous potential applications in the fields of genetic engineering, medicine, agriculture, and food technology, it ...
DOI:: 10.1016/j.ijbiomac.2018.12.169
: https://doi.org/10.1016/j.ijbiomac.2018.12.169

29. Accelerated host angiogenesis and immune responses by ion release from mesoporous bioactive glass

Author:: Yinghong ZhouYinghong Zhou and Shengwei Han contributed equally to this work and are co-first authors.; Shengwei Han; Lan Xiao; Pingping Han; Shengfang Wang; Jie He; Jiang Chang; Chengtie Wu; Yin Xiao
Source:: Journal of materials chemistry 2018 v.6 no.20 pp. 3274-3284
ISSN:: 2050-7518
Subject:: adverse effects; angiogenesis; biocompatible materials; blood vessels; chemistry; copper; enzymes; gene expression; genetic engineering; glass; human umbilical vein endothelial cells; hypoxia; hypoxia-inducible factor 1; immune response; inorganic ions; macrophages; medicine; mesenchymal stromal cells; myocardial ischemia; porous media; secretion; therapeutics; tissue repair; vascular endothelial growth factors
Abstract:: ... Angiogenesis represents a major focus for novel therapeutic approaches to the treatment and management of multiple pathological conditions, such as ischemic heart disease and critical-sized bone defect. The complex process of angiogenesis begins when cells within a tissue respond to hypoxia by increasing their production of vascular endothelial growth factor (VEGF). Loading biomaterials with angio ...
DOI:: 10.1039/c8tb00683k
: https://doi.org/10.1039/c8tb00683k

30. Applications of genetically engineered human pluripotent stem cell reporters in cardiac stem cell biology

Author:: Joe Z Zhang; Hongchao Guo; Joseph C Wu
Source:: Current opinion in biotechnology 2018 v.52 pp. 66-73
ISSN:: 0958-1669
Subject:: cardiovascular diseases; genetic engineering; humans; inheritance (genetics); medicine; stem cells; technology
Abstract:: ... The advent of human pluripotent stem cells (hPSCs) has benefited many fields, from regenerative medicine to disease modeling, with an especially profound effect in cardiac research. Coupled with other novel technologies in genome engineering, hPSCs offer a great opportunity to delineate human cardiac lineages, investigate inherited cardiovascular diseases, and assess the safety and efficacy of cel ...
DOI:: 10.1016/j.copbio.2018.03.002
PubMed:: 29579626
PubMed Central:: PMC6082724
: https://doi.org/10.1016/j.copbio.2018.03.002

31. Biomaterials-based 3D cell printing for next-generation therapeutics and diagnostics

Author:: Jinah Jang; Ju Young Park; Ge Gao; Dong-Woo Cho
Source:: Biomaterials 2018 v.156 pp. 88-106
ISSN:: 0142-9612
Subject:: biochemical compounds; biocompatible materials; bioprinting; diagnostic techniques; genetic engineering; humans; manufacturing; medicine; morphogenesis; polymerization; stem cells; therapeutics; tissue engineering; tissues
Abstract:: ... Building human tissues via 3D cell printing technology has received particular attention due to its process flexibility and versatility. This technology enables the recapitulation of unique features of human tissues and the all-in-one manufacturing process through the design of smart and advanced biomaterials and proper polymerization techniques. For the optimal engineering of tissues, a higher-or ...
DOI:: 10.1016/j.biomaterials.2017.11.030
: https://doi.org/10.1016/j.biomaterials.2017.11.030

32. Can Reprogramming of Overall Epigenetic Memory and Specific Parental Genomic Imprinting Memory within Donor Cell-Inherited Nuclear Genome be a Major Hindrance for the Somatic Cell Cloning of Mammals? – A Review

Author:: Marcin Samiec; Maria Skrzyszowska
Source:: Annals of animal science 2018 v.18 no.3 pp. 623-638
ISSN:: 2300-8733
Subject:: DNA; acetylation; animal science; chromatin; cytosine; demethylation; early development; gene expression; genetic engineering; genomics; germ cells; histones; humans; lysine; medicine; memory; nuclear genome; somatic cells; transcription (genetics)
Abstract:: ... Successful cloning of animals by somatic cell nuclear transfer (SCNT) requires epigenetic transcriptional reprogramming of the differentiated state of the donor cell nucleus to a totipotent embryonic ground state. It means that the donor nuclei must cease its own program of gene expression and restore a particular program of the embryonic genome expression regulation that is necessary for normal d ...
DOI:: 10.2478/aoas-2018-0015
: https://doi.org/10.2478/aoas-2018-0015

33. Generation of transgenic‐cloned Huanjiang Xiang pigs systemically expressing enhanced green fluorescent protein

Author:: Xiang‐Xing Zhu; Yi‐Zhi Zhong; Yao‐Wen Ge; Ke‐Huan Lu; Sheng‐Sheng Lu
Source:: Reproduction in domestic animals 2018 v.53 no.6 pp. 1546-1554
ISSN:: 0936-6768
Subject:: artificial skin; biotechnology; embryo (animal); fibroblasts; genetic engineering; genotyping; green fluorescent protein; medicine; miniature swine; piglets; somatic cells; transgenes; China
Abstract:: ... Huanjiang Xiang pig is a unique native minipig breed originating in Guangxi, China, and has great utility value in agriculture and biomedicine. Reproductive biotechnologies such as somatic cell nuclear transfer (SCNT) and SCNT‐mediated genetic modification show great potential value in genetic preservation and utilization of Huanjiang Xiang pigs. Our previous work has successfully produced cloned ...
DOI:: 10.1111/rda.13301
: https://doi.org/10.1111/rda.13301

34. Genetic tool development and systemic regulation in biosynthetic technology

Author:: Zhongxue Dai; Shangjie Zhang; Qiao Yang; Wenming Zhang; Xiujuan Qian; Weiliang Dong; Min Jiang; Fengxue Xin
Source:: Biotechnology for biofuels 2018 v.11 no.1 pp. 152
ISSN:: 1754-6834
Subject:: biosynthesis; chemical industry; computer science; electronics; energy; evolution; genetic engineering; genomics; issues and policy; mathematics; medicine; metabolic engineering; synthetic biology
Abstract:: ... With the increased development in research, innovation, and policy interest in recent years, biosynthetic technology has developed rapidly, which combines engineering, electronics, computer science, mathematics, and other disciplines based on classical genetic engineering and metabolic engineering. It gives a wider perspective and a deeper level to perceive the nature of life via cell mechanism, r ...
DOI:: 10.1186/s13068-018-1153-5
PubMed:: 29881457
PubMed Central:: PMC5984347
: https://doi.org/10.1186/s13068-018-1153-5

35. Genome engineering for microbial natural product discovery

Author:: Si-Sun Choi; Yohei Katsuyama; Linquan Bai; Zixin Deng; Yasuo Ohnishi; Eung-Soo Kim
Source:: Current opinion in microbiology 2018 v.45 pp. 53-60
ISSN:: 1369-5274
Subject:: biosynthesis; biotechnology; genetic engineering; genome mining; medicine; multigene family
Abstract:: ... The discovery and development of microbial natural products (MNPs) have played pivotal roles in the fields of human medicine and its related biotechnology sectors over the past several decades. The post-genomic era has witnessed the development of microbial genome mining approaches to isolate previously unsuspected MNP biosynthetic gene clusters (BGCs) hidden in the genome, followed by various BGC ...
DOI:: 10.1016/j.mib.2018.02.007
: https://doi.org/10.1016/j.mib.2018.02.007

36. Induced pluripotent stem cells: A new addition to the virologists armamentarium

Author:: Atichat Kuadkitkan; Nitwara Wikan; Duncan R. Smith
Source:: Journal of virological methods 2016 v.235 pp. 191-195
ISSN:: 0166-0934
Subject:: cell lines; genetic disorders; genetic engineering; induced pluripotent stem cells; medicine; neoplasms; tissue culture; virus replication; viruses
Abstract:: ... A significant amount of our understanding of the molecular events occurring during viral replication has originated from studies utilizing cell lines. These cell lines are normally obtained by the culture of samples from spontaneously occurring tumors or are derived by genetic manipulation of primary cells. The genetic events inducing immortalization and/or transformation to allow continual passag ...
DOI:: 10.1016/j.jviromet.2016.03.009
: https://doi.org/10.1016/j.jviromet.2016.03.009

37. Knowledge, Attitudes Toward, and Acceptability of Genetic Modification among Western Balkan University Students of Life Sciences (AGREE Study)

Author:: Vladica Veličković; Marko Jović; Ena Nalić; Aleksandar Višnjić; Olivera Radulović; Čedomir Šagrić; Milan Ćirić
Source:: Journal of the American College of Nutrition 2016 v.35 no.2 pp. 150-162
ISSN:: 1541-1087
Subject:: attitudes and opinions; biotechnology; college students; cross-sectional studies; data collection; developing countries; factor analysis; food industry; food production; gender; genetic engineering; medicine; models; principal component analysis; questionnaires; risk; Balkans
Abstract:: ... Background : There are still no data on the attitudes and acceptance of genetic modification (GM) food in European developing countries, such as the Western Balkan countries. The aim of the study was to assess the knowledge, attitudes, and acceptance of GM but also to shed light on the multifactorial process leading to acceptance of genetic modifications among Western Balkan students of life scien ...
DOI:: 10.1080/07315724.2014.1003115
: https://doi.org/10.1080/07315724.2014.1003115

38. Multifunctional Enzyme Packaging and Catalysis in the Qβ Protein Nanoparticle

Author:: Jason D. Fiedler; Maxwell R. Fishman; Steven D. Brown; Jolene Lau; M. G. Finn
Source:: Biomacromolecules 2018 v.19 no.10 pp. 3945-3957
ISSN:: 1526-4602
Subject:: Escherichia coli; catalytic activity; coat proteins; environmental factors; enzymes; genetic engineering; ligands; manufacturing; medicine; nanoparticles; packaging
Abstract:: ... The simultaneous expression in Escherichia coli cells of the Qβ virus-like particle (VLP) capsid protein and protein “cargo” tagged with a positively charged Rev peptide sequence leads to the spontaneous self-assembly of VLPs with multiple copies of the cargo inside. We report the packaging of four new enzymes with potential applications in medicine and chemical manufacturing. The captured enzymes ...
DOI:: 10.1021/acs.biomac.8b00885
: https://doi.org/10.1021/acs.biomac.8b00885

39. Production of squalene by microbes: an update

Author:: Wen Xu; Xi Ma; Yang Wang
Source:: World journal of microbiology & biotechnology 2016 v.32 no.12 pp. 195
ISSN:: 0959-3993
Subject:: liver; medicine; enzymes; hosts; industry; sharks; seed oils; microorganisms; biochemical pathways; consumer demand; plants (botany); triterpenoids; genetic engineering; squalene; Spermatophytina; antioxidants; anticarcinogenic activity
Abstract:: ... Squalene, a naturally occurring linear triterpene formed via MVA or MEP biosynthetic pathway, is widely distributed in microorganisms, plants and animals. At present, squalene is used extensively in the food, cosmetic and medicine industries because of its antioxidant, antistatic and anti-carcinogenic properties. Increased consumer demand has led to the development of microbial bioprocesses for th ...
DOI:: 10.1007/s11274-016-2155-8
: https://doi.org/10.1007/s11274-016-2155-8

40. Rational Design of Mouse Models for Cancer Research

Author:: Marietta Landgraf; Jacqui A. McGovern; Peter Friedl; Dietmar W. Hutmacher
Source:: Trends in biotechnology 2018 v.36 pp. 242-251
ISSN:: 0167-7799
Subject:: animal models; genetic engineering; genomics; human diseases; humans; medicine; mice; neoplasms; patients; therapeutics; tissue engineering
Abstract:: ... The laboratory mouse is widely considered as a valid and affordable model organism to study human disease. Attempts to improve the relevance of murine models for the investigation of human pathologies led to the development of various genetically engineered, xenograft and humanized mouse models. Nevertheless, most preclinical studies in mice suffer from insufficient predictive value when compared ...
DOI:: 10.1016/j.tibtech.2017.12.001
: https://doi.org/10.1016/j.tibtech.2017.12.001

41. The current perspectives of dromedary camel stem cells research

Author:: Islam M. Saadeldin; Ayman Abdel-Aziz Swelum; Faisal A. Alzahrani; Abdullah N. Alowaimer
Source:: International journal of veterinary science and medicine 2018 v.6 pp. S27
ISSN:: 2314-4599
Subject:: Camelus dromedarius; biomedical research; breeds; camels; dry environmental conditions; gene targeting; genetic engineering; genetic improvement; genome; germplasm conservation; meat; medicine; milk; milk production; models; stem cells; transgenic animals; West Asia
Abstract:: ... Camels have cultural value in the Arab society and are considered one of the most important animals in the Arabian Peninsula and arid environments, due to the distinct characteristics of their meat and milk. Moreover, there is a great interest in camel racing and beauty shows. Therefore, treatment of elite animals, increasing the number of camels as well as genetic improvement is an essential dema ...
DOI:: 10.1016/j.ijvsm.2018.01.002
PubMed:: 30761317
PubMed Central:: PMC6161867
: https://doi.org/10.1016/j.ijvsm.2018.01.002

42. A high-efficiency recombineering system with PCR-based ssDNA in Bacillus subtilis mediated by the native phage recombinase GP35

Author:: Zhaopeng Sun; Aihua Deng; Ting Hu; Jie Wu; Qinyun Sun; Hua Bai; Guoqiang Zhang; Tingyi Wen
Source:: Applied microbiology and biotechnology 2015 v.99 no.12 pp. 5151-5162
ISSN:: 0175-7598
Subject:: Bacillus subtilis; Escherichia coli; alpha-amylase; bacteria; bacteriophages; genes; genetic engineering; hosts; industry; kinship; loci; medicine; microbial physiology; nucleosides; phosphorylase; phylogeny; polymerase chain reaction; single-stranded DNA; uracil
Abstract:: ... Bacillus subtilis and its closely related species are important strains for industry, agriculture, and medicine. However, it is difficult to perform genetic manipulations using the endogenous recombination machinery. In many bacteria, phage recombineering systems have been employed to improve recombineering frequencies. To date, an efficient phage recombineering system for B. subtilis has not been ...
DOI:: 10.1007/s00253-015-6485-5
: https://doi.org/10.1007/s00253-015-6485-5

43. A perspective on the economic valorization of gene manipulated biotechnology: Past and future

Author:: Mirjam Knockaert; Sophie Manigart; Sofie Cattoir; Willy Verstraete
Source:: Biotechnology reports 2015 v.6 pp. 56-60
ISSN:: 2215-017X
Subject:: biotechnology; commercialization; crops; environmental sustainability; funding; genes; genetic engineering; industry; markets; medicine
Abstract:: ... Three distinct fields of gene manipulated biotechnology have so far been economically exploited: medical biotechnology, plant biotechnology and industrial biotechnology. This article analyzes the economic evolution and its drivers in the three fields over the past decades, highlighting strong divergences. Product and market characteristics, affecting firms’ financing options, are shown to be impor ...
DOI:: 10.1016/j.btre.2015.01.002
PubMed:: 28435808
PubMed Central:: PMC5374282
: https://doi.org/10.1016/j.btre.2015.01.002

44. Fusion expression of the PGLa-AM1 with native structure and evaluation of its anti-Helicobacter pylori activity

Author:: Xiaolin Zhang; Anmin Jiang; Guisheng Wang; Hao Yu; Banghua Qi; Youyi Xiong; Guoliang Zhou; Meisong Qin; Jinfeng Dou; Jianfei Wang
Source:: Applied microbiology and biotechnology 2017 v.101 no.14 pp. 5667-5675
ISSN:: 0175-7598
Subject:: Bombyx mori; Escherichia coli; Helicobacter pylori; Tobacco etch virus; amino acids; antibacterial properties; antibiotics; antimicrobial peptides; drugs; genes; genetic engineering; medicine; molecular weight; proteinases; therapeutics
Abstract:: ... Helicobacter pylori (H. pylori) shows increasingly enhanced resistance to various antibiotics, and its eradication has become a major problem in medicine. The antimicrobial peptide PGLa-AM1 is a short peptide with 22 amino acids and exhibits strong antibacterial activity. In this study, we investigated whether it has anti-H. pylori activity for the further development of anti-H. pylori drugs to re ...
DOI:: 10.1007/s00253-017-8302-9
: https://doi.org/10.1007/s00253-017-8302-9

45. Genetic Manipulation by Zinc-Finger Nucleases in Rat-Induced Pluripotent Stem Cells

Author:: Yang Sheng; Ding Shufang; Xu Qianhua; Li Xiong; Xiong Qiong
Source:: Cellular reprogramming 2017 v.19 no.3 pp. 180-188
ISSN:: 2152-4998
Subject:: Southern blotting; alcohol drinking; disease models; genes; genetic engineering; homologous recombination; human diseases; hypertension; induced pluripotent stem cells; kidney diseases; medicine; nucleases; rats
Abstract:: ... Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this species. But such tools do not yet exist for many rat strains, especially for disease model rat. The Sprague Dawley (SD) rat is an inbred disease model for hyper ...
DOI:: 10.1089/cell.2016.0028
: https://doi.org/10.1089/cell.2016.0028

46. Humoral immune responses against gonadotropin releasing hormone elicited by immunization with phage-peptide constructs obtained via phage display

Author:: Alexandre Samoylov; Anna Cochran; Bettina Schemera; Michelle Kutzler; Caitlin Donovan; Valery Petrenko; Frank Bartol; Tatiana Samoylova
Source:: Journal of biotechnology 2015 v.216 pp. 20-28
ISSN:: 0168-1656
Subject:: adjuvants; antibody formation; bacteriophages; blood serum; coat proteins; genetic engineering; gonadotropin-releasing hormone; humoral immunity; immunization; medicine; mice; neutralization; neutralizing antibodies; sequence homology; testosterone; vaccines; virion
Abstract:: ... Phage display is based on genetic engineering of phage coat proteins resulting in fusion peptides displayed on the surface of phage particles. The technology is widely used for generation of phages with novel characteristics for numerous applications in biomedicine and far beyond. The focus of this study was on development of phage-peptide constructs that stimulate production of antibodies against ...
DOI:: 10.1016/j.jbiotec.2015.10.001
: https://doi.org/10.1016/j.jbiotec.2015.10.001

47. Interaction of carbon nanohorns with plants: Uptake and biological effects

Author:: Mohamed H. Lahiani; Jihua Chen; Fahmida Irin; Alexander A. Puretzky; Micah J. Green; Mariya V. Khodakovskaya
Source:: Carbon 2015 v.81 pp. 607-619
ISSN:: 0008-6223
Subject:: Panicum virgatum; barley; bioactive properties; carbon; cell culture; corn; crops; genes; genetic engineering; horticulture; medicine; nanomaterials; plant growth substances; rice; soybeans; stress response; tobacco; tomatoes; transmission electron microscopy
Abstract:: ... Single-walled carbon nanohorns (SWCNHs) are a unique carbon-based nanomaterial with promising application in different fields including, medicine, genetic engineering and horticulture. Here, we investigated the biological response of six crop species (barley, corn, rice, soybean, switchgrass, tomato) and tobacco cell culture to the exposure of SWCNHs. We found that SWCNHs can activate seed germina ...
DOI:: 10.1016/j.carbon.2014.09.095
: https://doi.org/10.1016/j.carbon.2014.09.095

48. Recent developments and clinical studies utilizing engineered zinc finger nuclease technology

Author:: Young-Il Jo; Hyongbum Kim; Suresh Ramakrishna
Source:: Cellular and molecular life sciences 2015 v.72 no.20 pp. 3819-3830
ISSN:: 1420-682X
Subject:: DNA; biotechnology; clinical trials; cold stress; genes; genetic disorders; genetic engineering; homologous recombination; humans; medicine; mutants; nucleases; zinc finger motif
Abstract:: ... Efficient methods for creating targeted genetic modifications have long been sought for the investigation of gene function and the development of therapeutic modalities for various diseases, including genetic disorders. Although such modifications are possible using homologous recombination, the efficiency is extremely low. Zinc finger nucleases (ZFNs) are custom-designed artificial nucleases that ...
DOI:: 10.1007/s00018-015-1956-5
: https://doi.org/10.1007/s00018-015-1956-5

49. Effects of overexpression of AaWRKY1 on artemisinin biosynthesis in transgenic Artemisia annua plants

Author:: Junli Han; Hongzhen Wang; Anneli Lundgren; Peter E. Brodelius
Source:: Phytochemistry 2014 v.102 pp. 89-96
ISSN:: 0031-9422
Subject:: Artemisia annua; artemisinin; biosynthesis; gene expression regulation; gene overexpression; genetic engineering; medicine; transcription factors; transgenic plants
Abstract:: ... The effective anti-malarial medicine artemisinin is costly because of the low content in Artemisia annua. Genetic engineering of A. annua is one of the most promising approaches to improve the yield of artemisinin. In this work, the transcription factor AaWRKY1, which is thought to be involved in the regulation of artemisinin biosynthesis, was cloned from A. annua var. Chongqing and overexpressed ...
DOI:: 10.1016/j.phytochem.2014.02.011
: https://doi.org/10.1016/j.phytochem.2014.02.011

50. Genetically modified bacteriophages in applied microbiology

Author:: P. Bárdy; R. Pantůček; M. Benešík; J. Doškař
Source:: Journal of applied microbiology 2016 v.121 no.3 pp. 618-633
ISSN:: 1364-5072
Subject:: antibiotics; apoptosis; bacteria; bacterial infections; bacteriophages; biofilm; biosorbents; biotechnology; blood flow; enzymes; food industry; food pathogens; gene transfer; genes; genetic engineering; host range; mammals; medicine; microbial culture; microbial detection; models; molecular biology; vaccines; viability
Abstract:: ... Bacteriophages represent a simple viral model of basic research with many possibilities for practical application. Due to their ability to infect and kill bacteria, their potential in the treatment of bacterial infection has been examined since their discovery. With advances in molecular biology and gene engineering, the phage application spectrum has been expanded to various medical and biotechno ...
DOI:: 10.1111/jam.13207
: https://doi.org/10.1111/jam.13207

51. Leveraging Rules of Nonsense-Mediated mRNA Decay for Genome Engineering and Personalized Medicine

Author:: Maximilian W. Popp; Lynne E. Maquat
Source:: Cell 2016 v.165 pp. 1319-1322
ISSN:: 0092-8674
Subject:: diagnostic techniques; genes; genetic engineering; human health; medicine; messenger RNA; quality control
Abstract:: ... Nonsense-mediated mRNA decay (NMD) is a eukaryotic mRNA quality control and regulatory process that plays direct roles in human health and disease. In this Minireview, we discuss how understanding the molecular events that trigger NMD can facilitate strategic targeting of genes via CRISPR/Cas9 technologies and also inform disease diagnostics and treatments. ...
DOI:: 10.1016/j.cell.2016.05.053
PubMed:: 27259145
PubMed Central:: PMC4924582
: https://doi.org/10.1016/j.cell.2016.05.053

52. MSC-based VEGF gene therapy in rat myocardial infarction model using facial amphipathic bile acid-conjugated polyethyleneimine

Author:: Hyung-Ho Moon; Min Kyung Joo; Hyejung Mok; Minhyung Lee; Ki-Chul Hwang; Sung Wan Kim; Ji Hoon Jeong; Donghoon Choi; Sun Hwa Kim
Source:: Biomaterials 2014 v.35 no.5 pp. 1744-1754
ISSN:: 0142-9612
Subject:: bile; cell viability; cultured cells; gene therapy; genes; genetic engineering; infarction; medicine; models; myocardial infarction; polyethyleneimine; protein synthesis; rats; stem cells; tissues; transfection; vascular endothelial growth factors
Abstract:: ... Mesenchymal stem cells (MSCs) have attracted much attention in regenerative medicine owing to their apparent usefulness as multi-potent replacement cells. The potential of MSC therapy can be further improved by transforming MSCs with therapeutic genes that maximize the efficacy of gene therapy and their own therapeutic ability. Since most conventional transfection methodologies have shown marginal ...
DOI:: 10.1016/j.biomaterials.2013.11.019
: https://doi.org/10.1016/j.biomaterials.2013.11.019

53. Medicinal plant transcriptomes: the new gateways for accelerated understanding of plant secondary metabolism

Author:: Sandhya Tripathi; Jyoti Singh Jadaun; Muktesh Chandra; Neelam S. Sangwan
Source:: Plant genetic resources 2016 v.14 no.4 pp. 256-269
ISSN:: 1479-263X
Subject:: biochemical pathways; databases; evolution; genes; genetic engineering; high-throughput nucleotide sequencing; medicinal plants; medicinal properties; medicine; metabolism; nucleotide sequences; pharmaceutical industry; secondary metabolites; tissues; transcriptome; transcriptomics
Abstract:: ... Medicinal plants are the vital source of numerous structurally diverse pharmacologically active metabolites collectively called as secondary metabolites finding extensive applications in traditional systems of medicine and in pharmaceutical industries. Several distinctive and complex pathways operate in an interactive manner via metabolic networks that are responsible for the accumulation of such ...
DOI:: 10.1017/S1479262116000162
: https://doi.org/10.1017/S1479262116000162

54. Non-viral gene activated matrices for mesenchymal stem cells based tissue engineering of bone and cartilage

Author:: Sophie Raisin; Emmanuel Belamie; Marie Morille
Source:: Biomaterials 2016 v.104 pp. 223-237
ISSN:: 0142-9612
Subject:: RNA; bioactive properties; cartilage; clinical trials; complement; gene therapy; genes; genetic engineering; half life; medicine; plasmids; production costs; proteins; stem cells; tissue engineering; transfection
Abstract:: ... Recent regenerative medicine and tissue engineering strategies for bone and cartilage repair have led to fascinating progress of translation from basic research to clinical applications. In this context, the use of gene therapy is increasingly being considered as an important therapeutic modality and regenerative technique. Indeed, in the last 20 years, nucleic acids (plasmid DNA, interferent RNA) ...
DOI:: 10.1016/j.biomaterials.2016.07.017
: https://doi.org/10.1016/j.biomaterials.2016.07.017

55. Plant Based Bioreactors of Recombinant Cytokines (Review)

Author:: M. S. Burlakovskiy; V. V. Yemelyanov; L. A. Lutova
Source:: Applied biochemistry and microbiology 2016 v.52 no.2 pp. 121-137
ISSN:: 0003-6838
Subject:: bioreactors; cytokines; genetic engineering; genetically modified plants; immune response; medicine; neoplasms; pharmacology; polypeptides; production costs
Abstract:: ... Cytokines are a family of signaling polypeptides involved in intercellular interactions in the process of the immune response, as well as in the regulation of a number of normal physiological functions. Cytokines are used in medicine for the treatment of cancer, immune disorders, viral infections, and other socially significant diseases, but the extent of their use is limited by the high productio ...
DOI:: 10.1134/S0003683816020034
PubMed:: 32214409
PubMed Central:: PMC7087682
: https://doi.org/10.1134/S0003683816020034

56. Short O-GalNAc glycans: regulation and role in tumor development and clinical perspectives

Author:: Joanne Chia; Germaine Goh; Frederic Bard
Source:: Biochimica et biophysica acta 2016 v.1860 no.8 pp. 1623-1639
ISSN:: 0304-4165
Subject:: antibodies; antigens; biomarkers; cell membranes; enzymes; genetic engineering; glycosylation; medicine; neoplasm cells; neoplasms; physiological transport; polysaccharides; vaccine development
Abstract:: ... While the underlying causes of cancer are genetic modifications, changes in cellular states mediate cancer development. Tumor cells display markedly changed glycosylation states, of which the O-GalNAc glycans called the Tn and TF antigens are particularly common. How these antigens get over-expressed is not clear. The expression levels of glycosylation enzymes fail to explain it.We describe the re ...
DOI:: 10.1016/j.bbagen.2016.03.008
: https://doi.org/10.1016/j.bbagen.2016.03.008

57. Biological and Biomedical Applications of Engineered Nucleases

Author:: Yunzhi Pan; Li Xiao; Alice S. S. Li; Xu Zhang; Pierre Sirois; Jia Zhang; Kai Li
Source:: Molecular biotechnology 2013 v.55 no.1 pp. 54-62
ISSN:: 1073-6085
Subject:: DNA repair; gene therapy; genes; genetic engineering; human diseases; medicine; nucleases
Abstract:: ... The development of engineered nucleases is the fruit of a new technological approach developed in the last two decades which has led to significant benefits on genome engineering, particularly on gene therapy. These applications enable efficient and specific genetic modifications via the induction of a double-strand break (DSB) in a specific genomic target sequence, followed by the homology-direct ...
DOI:: 10.1007/s12033-012-9613-9
PubMed:: 23089945
: https://doi.org/10.1007/s12033-012-9613-9

58. Biotechnological aspects of cytoskeletal regulation in plants

Author:: George Komis; Ivan Luptovciak; Anna Doskocilova; Jozef Samaj
Source:: Biotechnology Advances 2015 v.33 pp. 1043-1062
ISSN:: 0734-9750
Subject:: Vinca; alkaloids; biofuels; biosynthesis; cell differentiation; cell division; cell walls; cellulose; crops; cytoskeletal proteins; defense mechanisms; environmental factors; eukaryotic cells; fiber quality; fuel production; genetic engineering; growth and development; medicine; microfilaments; microtubules; pathogens; plant growth; prokaryotic cells; synthesis; toxoids; wood fibers
Abstract:: ... The cytoskeleton is a protein-based intracellular superstructure that evolved early after the appearance of bacterial prokaryotes. Eventually cytoskeletal proteins and their macromolecular assemblies were established in eukaryotes and assumed critical roles in cell movements, intracellular organization, cell division and cell differentiation. In biomedicine the small-molecules targeting cytoskelet ...
DOI:: 10.1016/j.biotechadv.2015.03.008
: https://doi.org/10.1016/j.biotechadv.2015.03.008

59. Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

Author:: Zhonggang Hou; Yan Zhang; Nicholas E. Propson; Sara E. Howden; Li-Fang Chu; Erik J. Sontheimer; James A. Thomson
Source:: Proceedings of the National Academy of Sciences of the United States of America 2013 v.110 no.39 pp. 15644-15649
ISSN:: 0027-8424
Subject:: DNA; Neisseria meningitidis; RNA; Streptococcus pyogenes; Streptococcus thermophilus; bacteria; biomedical research; eukaryotic cells; gene targeting; genes; genetic engineering; medicine; proteins; stem cells
Abstract:: ... Genome engineering in human pluripotent stem cells (hPSCs) holds great promise for biomedical research and regenerative medicine. Recently, an RNA-guided, DNA-cleaving interference pathway from bacteria [the type II clustered, regularly interspaced, short palindromic repeats (CRISPR)-CRISPR-associated (Cas) pathway] has been adapted for use in eukaryotic cells, greatly facilitating genome editing. ...
DOI:: 10.1073/pnas.1313587110
PubMed:: 23940360
PubMed Central:: PMC3785731
: https://doi.org/10.1073/pnas.1313587110

60. Electroporation-based applications in biotechnology

Author:: Tadej Kotnik; Wolfgang Frey; Martin Sack; Saša Haberl Meglič; Matjaž Peterka; Damijan Miklavčič
Source:: Trends in biotechnology 2015 v.33 no.8 pp. 480-488
ISSN:: 0167-7799
Subject:: biochemical compounds; biomass; biotechnology; drying; electroporation; genetic engineering; medicine; microorganisms
Abstract:: ... Electroporation is already an established technique in several areas of medicine, but many of its biotechnological applications have only started to emerge; we review here some of the most promising. We outline electroporation as a phenomenon and then proceed to applications, first outlining the best established – the use of reversible electroporation for heritable genetic modification of microorg ...
DOI:: 10.1016/j.tibtech.2015.06.002
: https://doi.org/10.1016/j.tibtech.2015.06.002

61. Engineering mesenchymal stem cells for regenerative medicine and drug delivery

Author:: Ji Sun Park; Smruthi Suryaprakash; Yeh-Hsing Lao; Kam W. Leong
Source:: Methods 2015 v.84 pp. 3-16
ISSN:: 1046-2023
Subject:: biopharmaceuticals; genetic engineering; immunosuppression; medicine; nanoparticles; stem cells; therapeutics; tropisms
Abstract:: ... Researchers have applied mesenchymal stem cells (MSC) to a variety of therapeutic scenarios by harnessing their multipotent, regenerative, and immunosuppressive properties with tropisms toward inflamed, hypoxic, and cancerous sites. Although MSC-based therapies have been shown to be safe and effective to a certain degree, the efficacy remains low in most cases when MSC are applied alone. To enhanc ...
DOI:: 10.1016/j.ymeth.2015.03.002
PubMed:: 25770356
PubMed Central:: PMC4526354
: https://doi.org/10.1016/j.ymeth.2015.03.002

62. Fermentative production of glycine betaine and trehalose from acid whey using Actinopolyspora halophila (MTCC 263)

Author:: Jayaranjan R. Kar; John E. Hallsworth; Rekha S. Singhal
Source:: Environmental technology & innovation 2015 v.3 pp. 68-76
ISSN:: 2352-1864
Subject:: Actinopolyspora halophila; acidity; betaine; biochemical oxygen demand; bioinformatics; bioprocessing; confectionery industry; dairy industry; environmental technology; feeds; genetic engineering; halophiles; landfills; manufacturing; medicine; nitrogen; production costs; response surface methodology; soil; trehalose; whey; yogurt
Abstract:: ... Acid whey has become a major concern especially in dairy industry manufacturing Greek yoghurt. Proper disposal of acid whey is essential as it not only increases the BOD of water but also increases the acidity when disposed of in landfill, rendering soil barren and unsuitable for cultivation. Effluent (acid-whey) treatment increases the cost of production. The vast quantities of acid whey that are ...
DOI:: 10.1016/j.eti.2015.02.001
: https://doi.org/10.1016/j.eti.2015.02.001

63. Lost signature: progress and failures in in vivo tracking of implanted stem cells

Author:: K. von der Haar; A. Lavrentieva; F. Stahl; T. Scheper; C. Blume
Source:: Applied microbiology and biotechnology 2015 v.99 no.23 pp. 9907-9922
ISSN:: 0175-7598
Subject:: carcinogens; computed tomography; genetic engineering; image analysis; magnetic resonance imaging; medical treatment; medicine; monitoring; positron-emission tomography; stem cells
Abstract:: ... Stem cell therapy as a part of regenerative medicine provides promising approaches for the treatment of injuries and diseases. The increasing use of mesenchymal stem cells in various medical treatments created the demand for long-term in vivo cell tracking methods. Therefore, it is necessary to analyze post-transplantational survival, biodistribution, and engraftment of cells. Furthermore, stem ce ...
DOI:: 10.1007/s00253-015-6965-7
: https://doi.org/10.1007/s00253-015-6965-7

64. MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via Cationized Pleurotus eryngii Polysaccharide-based Nanotransfection

Author:: Deng Wenwen; Cao Xia; Chen Jingjing; Zhang Zhijian; Yu Qingtong; Wang Yan; Shao Genbao; Zhou Jie; Gao Xiangdong; Yu Jiangnan; Xu Ximing
Source:: ACS Applied Materials & Interfaces 2015 v.7 no.34 pp. 18957-18966
ISSN:: 1944-8252
Subject:: Pleurotus eryngii; genetic engineering; humans; induced pluripotent stem cells; medicine; microRNA; nanoparticles; particle size; physicochemical properties; plasmids; polysaccharides; transcription factors; umbilical cord
Abstract:: ... Induced pluripotent stem cells (iPSCs), resulting from the forced expression of cocktails out of transcription factors, such as Oct4, Sox2, Klf4, and c-Myc (OSKM), has shown tremendous potential in regenerative medicine. Although rapid progress has been made recently in the generation of iPSCs, the safety and efficiency remain key issues for further application. In this work, microRNA 302-367 was ...
DOI:: 10.1021/acsami.5b06768
: https://doi.org/10.1021/acsami.5b06768

65. Stress, adaptation, and speciation in the evolution of the blind mole rat, Spalax, in Israel

Author:: Eviatar Nevo
Source:: Molecular phylogenetics and evolution 2013 v.66 no.2 pp. 515-525
ISSN:: 1055-7903
Subject:: Spalax; burrows; evolution; genetic engineering; genome; hypercapnia; hypoxia; medicine; models; mole rats; mutation; pathogenicity; predators; proteomics; space flight; Israel
Abstract:: ... Environmental stress played a major role in the evolution of the blind mole rat superspecies Spalax ehrenbergi, affecting its adaptive evolution and ecological speciation underground. Spalax is safeguarded all of its life underground from aboveground climatic fluctuations and predators. However, it encounters multiple stresses in its underground burrows including darkness, energetics, hypoxia, hyp ...
DOI:: 10.1016/j.ympev.2012.09.008
PubMed:: 23032572
: https://doi.org/10.1016/j.ympev.2012.09.008

66. Development and Applications of CRISPR-Cas9 for Genome Engineering

Author:: Patrick D. Hsu; Eric S. Lander; Feng Zhang
Source:: Cell 2014 v.157 pp. 1262-1278
ISSN:: 0092-8674
Subject:: DNA; RNA; biotechnology; genetic engineering; genetic variation; genome; mammals; medicine; nucleotide sequences; phenotype; translation (genetics)
Abstract:: ... Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 can be guided to specific locations within complex genomes by a short RNA search string. Using this system, DNA sequences within the endogenous genome a ...
DOI:: 10.1016/j.cell.2014.05.010
PubMed:: 24906146
PubMed Central:: PMC4343198
: https://doi.org/10.1016/j.cell.2014.05.010

67. Efficient Generation of Hepatic Cells from Multipotent Adult Mouse Germ-Line Stem Cells Using an OP9 Co-Culture System

Author:: Streckfuss-Bömeke Katrin; Jende Jörg; Cheng I-Fen; Hasenfuss Gerd; Guan Kaomei
Source:: Cellular reprogramming 2014 v.16 no.1 pp. 65-76
ISSN:: 2152-4998
Subject:: GATA transcription factors; adult stem cells; adults; albumins; coculture; embryonic stem cells; gelatin; genetic engineering; glycogen; hepatocytes; humans; liver; low density lipoprotein; medicine; mice; models; secretion; testes; urea
Abstract:: ... On the basis of their self-renewal capacity and their ability to differentiate into derivatives of all three germ layers, germ line–derived multipotent adult stem cells (maGSCs) from mouse testis might serve as one of preferable sources for pluripotent stem cells in regenerative medicine. In our study, we aimed for an efficient hepatic differentiation protocol that is applicable for both maGSCs an ...
DOI:: 10.1089/cell.2013.0057
PubMed:: 24380658
PubMed Central:: PMC3920844
: https://doi.org/10.1089/cell.2013.0057

68. Histone deacetylase 8 suppresses osteogenic differentiation of bone marrow stromal cells by inhibiting histone H3K9 acetylation and RUNX2 activity

Author:: Yu Fu; Ping Zhang; Jie Ge; Jie Cheng; Weijie Dong; Hua Yuan; Yifei Du; Mifang Yang; Ruoxing Sun; Hongbing Jiang
Source:: international journal of biochemistry & cell biology 2014 v.54 pp. 68-77
ISSN:: 1357-2725
Subject:: acetylation; alkaline phosphatase; bone formation; bone marrow; epigenetics; genes; genetic engineering; histone deacetylase; histones; lysine; medicine; osteoblasts; osteocalcin; osteopontin; rats; small interfering RNA; stem cells; stromal cells; transcription (genetics); valproic acid
Abstract:: ... Bone marrow stromal cells (BMSCs) are multipotent progenitor cells with capacities to differentiate into the various cell types and hold great promise in regenerative medicine. The regulatory roles of histone deacetylases (HDACs) in osteoblast differentiation process have been increasingly recognized; however, little is known about the precise roles of HDAC8 in the osteogenic differentiation of BM ...
DOI:: 10.1016/j.biocel.2014.07.003
: https://doi.org/10.1016/j.biocel.2014.07.003

69. Investigation of Phase Separation Behavior and Formation of Plasmonic Nanocomposites from Polypeptide-Gold Nanorod Nanoassemblies

Author:: Huang Huang-Chiao; Nanda Alisha; Rege Kaushal
Source:: Langmuir 2012 v.28 no.16 pp. 6645-6655
ISSN:: 1520-5827
Subject:: cetyltrimethylammonium bromide; dispersions; drugs; genetic engineering; gold; irradiation; mathematical models; medicine; nanocarriers; nanocomposites; nanorods; physicochemical properties; polypeptides; separation; surfactants; temperature; tissue engineering
Abstract:: ... Genetically engineered elastin-like polypeptides (ELP) can be interfaced with cetyltrimethyl ammonium bromide (CTAB)-stabilized gold nanorods (GNRs) resulting in the formation of stable dispersions (nanoassemblies). Increasing the dispersion temperature beyond the ELP transition temperature results in phase separation and formation of solid-phase ELP-GNR matrices (nanocomposites). Here, we investi ...
DOI:: 10.1021/la203340y
: https://doi.org/10.1021/la203340y

70. Neurogenesis and vascularization of the damaged brain using a lactate-releasing biomimetic scaffold

Author:: Zaida Álvarez; Oscar Castaño; Alba A. Castells; Miguel A. Mateos-Timoneda; Josep A. Planell; Elisabeth Engel; Soledad Alcántara
Source:: Biomaterials 2014 v.35 no.17 pp. 4769-4781
ISSN:: 0142-9612
Subject:: alternative fuels; bioactive compounds; biocompatible materials; biomimetics; brain; brain damage; genetic engineering; growth factors; lactic acid; medicine; mice; nanofibers; neurogenesis; neurons; stem cells; topology
Abstract:: ... Regenerative medicine strategies to promote recovery following traumatic brain injuries are currently focused on the use of biomaterials as delivery systems for cells or bioactive molecules. This study shows that cell-free biomimetic scaffolds consisting of radially aligned electrospun poly-l/dl lactic acid (PLA70/30) nanofibers release l-lactate and reproduce the 3D organization and supportive fu ...
DOI:: 10.1016/j.biomaterials.2014.02.051
: https://doi.org/10.1016/j.biomaterials.2014.02.051

71. Polymersome surface decoration by an EGFP fusion protein employing Cecropin A as peptide âanchorâ

Author:: Muhammad Noor; Tamara Dworeck; Alexander Schenk; Pravin Shinde; Marco Fioroni; Ulrich Schwaneberg
Source:: Journal of biotechnology 2012 v.157 no.1 pp. 31-37
ISSN:: 0168-1656
Subject:: antibacterial proteins; biotechnology; catalytic activity; chemical bonding; composite polymers; drugs; facilitated diffusion; fluorescence; genetic engineering; green fluorescent protein; medicine; peptides; synthetic biology
Abstract:: ... Polymer based nanocompartments have potential applications in synthetic biology, medicine (drug release) and industrial biotechnology (chiral nanoreactors, multistep syntheses, selective product recovery). A step towards the aforementioned goals is the polymer membrane functionalization through covalent bonding of chemical anchors or insertion of proteins/peptides, to obtain specific properties li ...
DOI:: 10.1016/j.jbiotec.2011.10.005
: https://doi.org/10.1016/j.jbiotec.2011.10.005

72. Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases

Author:: Hauschild, Janet; Petersen, Bjoern; Santiago, Yolanda; Queisser, Anna-Lisa; Carnwath, Joseph W.; Lucas-Hahn, Andrea; Zhang, Lei; Meng, Xiangdong; Gregory, Philip D.; Schwinzer, Reinhard; Cost, Gregory J.; Niemann, Heiner
Source:: Proceedings of the National Academy of Sciences of the United States of America 2011 v.108 no.29 pp. 12013-12017
ISSN:: 0027-8424
Subject:: complement; epitopes; fetus; fibroblasts; fruit flies; galactose; gene targeting; genes; genetic engineering; laboratory animals; medicine; mice; nucleases; phenotype; plasmids; progeny; rats; somatic cells; swine
Abstract:: ... Zinc-finger nucleases (ZFNs) are powerful tools for producing gene knockouts (KOs) with high efficiency. Whereas ZFN-mediated gene disruption has been demonstrated in laboratory animals such as mice, rats, and fruit flies, ZFNs have not been used to disrupt an endogenous gene in any large domestic species. Here we used ZFNs to induce a biallelic knockout of the porcine α1,3-galactosyltransferase ( ...
DOI:: 10.1073/pnas.1106422108
PubMed:: 21730124
PubMed Central:: PMC3141985
: https://doi.org/10.1073/pnas.1106422108

73. New Tool for Genome Surgery

Author:: John van der Oost
Source:: Science 2013 v.339 no.6121 pp. 768-770
ISSN:: 0036-8075
Subject:: DNA; binding sites; gene therapy; genes; genetic engineering; humans; medicine; mutation; nucleases; nucleotide sequences; patients
Abstract:: ... Gene therapy is the holy grail of human medicine. Many diseases are caused by a defective gene, sometimes with a mutation as subtle as a single-nucleotide variation. Before restoration of such a mutation in a patient's genome can take place, the target nucleotide sequence has to be cleaved at a single position, out of 3 billion possibilities. This degree of precise surgery requires an enzyme with ...
DOI:: 10.1126/science.1234726
: https://doi.org/10.1126/science.1234726

74. Progress towards the ‘Golden Age’ of biotechnology

Author:: KMA Gartland; F Bruschi; M Dundar; PB Gahan; MP Viola Magni; Y Akbarova
Source:: Current opinion in biotechnology 2013 v.24 pp. S6
ISSN:: 0958-1669
Subject:: Agrobacterium; Europeans; antibiotics; antimalarials; bacterial infections; bioinformatics; biolistics; bioplastics; biotechnology; carbon; climate change; cold tolerance; detergents; diagnostic techniques; drought tolerance; economic development; energy crops; epigenetics; feedstocks; food production; food security; genes; genetic disorders; genetic engineering; human diseases; medicine; modified starch; nucleic acids; pathogens; polymerase chain reaction; temperature; transcriptomics; vaccines
Abstract:: ... Biotechnology uses substances, materials or extracts derived from living cells, employing 22 million Europeans in a €1.5Tn endeavour, being the premier global economic growth opportunity this century. Significant advances have been made in red biotechnology using pharmaceutically and medically relevant applications, green biotechnology developing agricultural and environmental tools and white biot ...
DOI:: 10.1016/j.copbio.2013.05.011
: https://doi.org/10.1016/j.copbio.2013.05.011

75. Recent advancements of graphene in biomedicine

Author:: Huacheng Zhang; George Grüner; Yanli Zhao
Source:: Journal of materials chemistry B 2013 v.1 no.20 pp. 2542-2567
ISSN:: 2050-7518
Subject:: antibacterial properties; biocompatibility; bioimaging; biotechnology; cytotoxicity; drugs; genetic engineering; graphene; medicine; nanomaterials; neoplasms; optical properties; phototherapy; tissue engineering
Abstract:: ... Graphene, as a rising star in the field of nanomaterials, possesses a unique planar structure and exceptional electronic, mechanical, and optical properties. The material has attracted tremendous interest not only for its intrinsic properties but also promising application opportunities in a wide range of technologies and markets. This review specifically summarizes recent research advancements of ...
DOI:: 10.1039/c3tb20405g
: https://doi.org/10.1039/c3tb20405g

76. Technical advances to genetically engineering human embryonic stem cells

Author:: Julien Jean Pierre Maury; Andre Boon-Hwa Choo; Ken Kwok-Keung Chan
Source:: Integrative biology 2011 v.3 no.7 pp. 717-723
ISSN:: 1757-9708
Subject:: DNA; Lentivirus; biomedical research; embryonic stem cells; genes; genetic engineering; human development; humans; medicine; models; nucleases; nucleotide sequences; therapeutics; transposases; zinc finger motif
Abstract:: ... Human embryonic stem cells (hESC) are important to basic scientific research as an in vitro model system for the study of human development and to clinical research as an invaluable cell source for regenerative medicine. The ability to genetically engineer hESC is a critical resource as it facilitates many fundamental studies to understand gene regulation and cell development. These techniques inc ...
DOI:: 10.1039/c1ib00019e
: https://doi.org/10.1039/c1ib00019e

77. The interface between plant metabolic engineering and human health

Author:: Cathie Martin
Source:: Current opinion in biotechnology 2013 v.24 pp. 344-353
ISSN:: 0958-1669
Subject:: chronic diseases; crops; diet; dietary recommendations; food matrix; genetic engineering; genetic variation; human health; mechanism of action; medicine; metabolic engineering; nutritive value; risk reduction; vegetables
Abstract:: ... The data on the benefits of consuming high levels of phytonutrients in fruit and vegetables to prevent or ameliorate chronic disease are very persuasive. To underpin reliable dietary recommendations and future campaigns for preventive medicine, significant fundamental research is required to define phytonutrients, their physiological effects following consumption, their mechanisms of action, the i ...
DOI:: 10.1016/j.copbio.2012.11.005
: https://doi.org/10.1016/j.copbio.2012.11.005

78. Construction of kanamycin B overproducing strain by genetic engineering of Streptomyces tenebrarius

Author:: Xianpu Ni; Dan Li; Lihua Yang; Tingjiao Huang; Hao Li; Huanzhang Xia
Source:: Applied microbiology and biotechnology 2011 v.89 no.3 pp. 723-731
ISSN:: 0175-7598
Subject:: Streptomyces tenebrarius; apramycin; biochemical pathways; biosynthesis; genetic engineering; hydrolysis; manufacturing; medicine; multigene family; tobramycin
Abstract:: ... Genetic engineering as an important approach to strain optimization has received wide recognition. Recent advances in the studies on the biosynthetic pathways and gene clusters of Streptomyces make stain optimization by genetic alteration possible. Kanamycin B is a key intermediate in the manufacture of the important medicines dibekacin and arbekacin, which belong to a class of antibiotics known a ...
DOI:: 10.1007/s00253-010-2908-5
PubMed:: 20936279
: https://doi.org/10.1007/s00253-010-2908-5

79. Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease

Author:: Grizot, Sylvestre; Smith, Julianne; Daboussi, Fayza; Prieto, Jesús; Redondo, Pilar; Merino, Nekane; Villate, Maider; Thomas, Séverine; Lemaire, Laetitia; Montoya, Guillermo; Blanco, Francisco J.; Pâques, Frédéric; Duchateau, Philippe
Source:: Nucleic acids research 2009 v.37 no.16 pp. 5405-5419
ISSN:: 0305-1048
Subject:: chemical elements; engineering; gene therapy; genes; genetic engineering; genotoxicity; humans; loci; medicine; mutation; nucleic acids; severe combined immunodeficiency; viruses
Abstract:: ... Sequence-specific endonucleases recognizing long target sequences are emerging as powerful tools for genome engineering. These endonucleases could be used to correct deleterious mutations or to inactivate viruses, in a new approach to molecular medicine. However, such applications are highly demanding in terms of safety. Mutations in the human RAG1 gene cause severe combined immunodeficiency (SCID ...
DOI:: 10.1093/nar/gkp548
PubMed:: 19584299
PubMed Central:: PMC2760784
: https://doi.org/10.1093/nar/gkp548

80. Glycosidases: a key to tailored carbohydrates

Author:: Bojarová, Pavla; Křen, Vladimír
Source:: Trends in biotechnology 2009 v.27 no.4 pp. 199-209
ISSN:: 0167-7799
Subject:: galactosides; genetic engineering; glycosidases; medicine; mutants; proteomics; screening
Abstract:: ... In recent years, carbohydrate-processing enzymes have become the enzymes of choice in many applications thanks to their stereoselectivity and efficiency. This review presents recent developments in glycosidase-catalyzed synthesis via two complementary approaches: the use of wild-type enzymes with engineered substrates, and mutant glycosidases. Genetic engineering has recently produced glucuronyl s ...
DOI:: 10.1016/j.tibtech.2008.12.003
: https://doi.org/10.1016/j.tibtech.2008.12.003

81. Engineering graded tissue interfaces

Author:: Phillips, Jennifer E.; Burns, Kellie L.; Le Doux, Joseph M.; Guldberg, Robert E.; García, Andrés J.
Source:: Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.34 pp. 12170-12175
ISSN:: 0027-8424
Subject:: Retroviridae; fibroblasts; gene transfer; genetic engineering; lysine; mechanical properties; medicine; mineralization; phenotype; sowing; tissue engineering; transcription factors
Abstract:: ... Interfacial zones between tissues provide specialized, transitional junctions central to normal tissue function. Regenerative medicine strategies focused on multiple cell types and/or bi/tri-layered scaffolds do not provide continuously graded interfaces, severely limiting the integration and biological performance of engineered tissue substitutes. Inspired by the bone-soft tissue interface, we de ...
DOI:: 10.1073/pnas.0801988105
PubMed:: 18719120
PubMed Central:: PMC2527884
: https://doi.org/10.1073/pnas.0801988105

82. Highly efficient transient gene expression and gene targeting in primate embryonic stem cells with helper-dependent adenoviral vectors

Author:: Suzuki, Keiichiro; Mitsui, Kaoru; Aizawa, Emi; Hasegawa, Kouichi; Kawase, Eihachiro; Yamagishi, Toshiyuki; Shimizu, Yoshihiko; Suemori, Hirofumi; Nakatsuji, Norio; Mitani, Kohnosuke
Source:: Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.37 pp. 13781-13786
ISSN:: 0027-8424
Subject:: Macaca fascicularis; clinical trials; embryonic stem cells; gene expression; gene targeting; gene transfer; genes; genetic engineering; homologous recombination; humans; medicine; mice
Abstract:: ... Human embryonic stem (hES) cells are regarded as a potentially unlimited source of cellular materials for regenerative medicine. For biological studies and clinical applications using primate ES cells, the development of a general strategy to obtain efficient gene delivery and genetic manipulation, especially gene targeting via homologous recombination (HR), would be of paramount importance. Howev ...
DOI:: 10.1073/pnas.0806976105
PubMed:: 18768795
PubMed Central:: PMC2544531
: https://doi.org/10.1073/pnas.0806976105

83. Unraveling the transcriptional network controlling ES cell pluripotency

Author:: Sridhar Rao; Stuart H Orkin
Source:: Genome biology 2006 v.7 no.8 pp. 1216
ISSN:: 1474-760X
Subject:: embryonic stem cells; genetic engineering; medicine; transcription (genetics)
Abstract:: ... Embryonic stem cells (ES cells) are powerful tools for genetic engineering and hold significant potential for regenerative medicine. Recent work provides new insights into ES cell pluripotency and delineates separate transcriptional pathways in ES cells for maintenance of the undifferentiated state and for self-renewal. ...
DOI:: 10.1186/gb-2006-7-8-230
PubMed:: 16942632
PubMed Central:: PMC1779601
: https://doi.org/10.1186/gb-2006-7-8-230

84. Cloning of Fish Enzymes and Other Fish Protein Genes

Author:: Martin Macouzet; Benjamin K. Simpson; Byong H. Lee
Source:: Critical reviews in biotechnology 1999 v.19 no.3 pp. 179-196
ISSN:: 1549-7801
Subject:: antibiotics; antifreeze proteins; aquaculture; fish; fluorescent proteins; food processing; foods; gene overexpression; genes; genetic engineering; hormones; mammals; medicine; metabolism; microorganisms; polypeptides; proteinases; recombinant DNA
Abstract:: ... Fish metabolism needs special enzymes that have maximum activity at very different conditions than their mammalian counterparts. Due to the differences in activity, these enzymes, especially cold-adapted proteases, could be used advantageously for the production of some foods. In addition to the enzymes, this review describes some other unique fish polypeptides such as antifreeze proteins, fluores ...
DOI:: 10.1080/0738-859991229233
PubMed:: 10576929
: https://doi.org/10.1080/0738-859991229233

85. Ethical concerns and risk perceptions associated with different applications of genetic engineering: interrelationships with the perceived need for regulation of the technology

Author:: Frewer, L.J.; Shepherd, R.
Source:: Agriculture and human values 1995 v.12 no.1 pp. 48-57
ISSN:: 0889-048X
Subject:: laws and regulations; risk; public opinion; medicine; ethics; genetic engineering; food production; technology
DOI:: 10.1007/BF02218074
: https://doi.org/10.1007/BF02218074

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1. Direct Cell Radiolabeling for in Vivo Cell Tracking with PET and SPECT Imaging

2. Biotechnological applications of Bacillus licheniformis

3. The role of genetic manipulation and in situ modifications on production of bacterial nanocellulose: A review

4. Transduction Enhancers Enable Efficient Human Adenovirus Type 5-Mediated Gene Transfer into Human Multipotent Mesenchymal Stromal Cells

5. Biotechnology of camptothecin production in Nothapodytes nimmoniana, Ophiorrhiza sp. and Camptotheca acuminata

6. Breeding of a cordycepin-resistant and adenosine kinase-deficient sake yeast strain that accumulates high levels of S-adenosylmethionine

7. Calcium carbonate supplementation to chorioallantoic membranes improves hatchability in shell-less chick embryo culture

8. Current strategies in tailoring methods for engineered exosomes and future avenues in biomedical applications

9. De novo assembly and comparative transcriptome analysis: novel insights into terpenoid biosynthesis in Chamaemelum nobile L.

10. Efficient plant regeneration and histological evaluations of regenerants through organogenesis and somatic embryogenesis in Spermacoce hispida L.—An underutilized medicinally important plant

11. High Aspect Ratio and Light-Sensitive Micropillars Based on a Semiconducting Polymer Optically Regulate Neuronal Growth

12. Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

13. Research advances on arachidonic acid production by fermentation and genetic modification of Mortierella alpina

14. A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology

15. Bacteria and cancer: Different sides of the same coin

16. Genetically Engineered Adipose Mesenchymal Stem Cells Using HIV-Based Lentiviral Vectors as Gene Therapy for Autoimmune Diseases

17. Individual identification and marking techniques for zebrafish

18. Innovations in CRISPR technology

19. Optogenetics sheds new light on tissue engineering and regenerative medicine

20. Polyhydroxybutyrate production from marine source and its application

21. 182 Establishment of expanded potential embryonic stem cell lines from porcine embryos

22. An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells

23. Bacillus thuringiensis: a successful insecticide with new environmental features and tidings

24. Bacteriophages immobilized on electrospun cellulose microfibers by non-specific adsorption, protein–ligand binding, and electrostatic interactions

25. Extremophilic exopolysaccharides: A review and new perspectives on engineering strategies and applications

26. Genetic circuitry for personalized human cell therapy

27. Genetic platforms for heterologous expression of microbial natural products

28. Purification and characterization of a cystatin like thiol protease inhibitor from Brassica nigra

29. Accelerated host angiogenesis and immune responses by ion release from mesoporous bioactive glass

30. Applications of genetically engineered human pluripotent stem cell reporters in cardiac stem cell biology

31. Biomaterials-based 3D cell printing for next-generation therapeutics and diagnostics

32. Can Reprogramming of Overall Epigenetic Memory and Specific Parental Genomic Imprinting Memory within Donor Cell-Inherited Nuclear Genome be a Major Hindrance for the Somatic Cell Cloning of Mammals? – A Review

33. Generation of transgenic‐cloned Huanjiang Xiang pigs systemically expressing enhanced green fluorescent protein

34. Genetic tool development and systemic regulation in biosynthetic technology

35. Genome engineering for microbial natural product discovery

36. Induced pluripotent stem cells: A new addition to the virologists armamentarium

37. Knowledge, Attitudes Toward, and Acceptability of Genetic Modification among Western Balkan University Students of Life Sciences (AGREE Study)

38. Multifunctional Enzyme Packaging and Catalysis in the Qβ Protein Nanoparticle

39. Production of squalene by microbes: an update

40. Rational Design of Mouse Models for Cancer Research

41. The current perspectives of dromedary camel stem cells research

42. A high-efficiency recombineering system with PCR-based ssDNA in Bacillus subtilis mediated by the native phage recombinase GP35

43. A perspective on the economic valorization of gene manipulated biotechnology: Past and future

44. Fusion expression of the PGLa-AM1 with native structure and evaluation of its anti-Helicobacter pylori activity

45. Genetic Manipulation by Zinc-Finger Nucleases in Rat-Induced Pluripotent Stem Cells

46. Humoral immune responses against gonadotropin releasing hormone elicited by immunization with phage-peptide constructs obtained via phage display

47. Interaction of carbon nanohorns with plants: Uptake and biological effects

48. Recent developments and clinical studies utilizing engineered zinc finger nuclease technology

49. Effects of overexpression of AaWRKY1 on artemisinin biosynthesis in transgenic Artemisia annua plants

50. Genetically modified bacteriophages in applied microbiology

51. Leveraging Rules of Nonsense-Mediated mRNA Decay for Genome Engineering and Personalized Medicine

52. MSC-based VEGF gene therapy in rat myocardial infarction model using facial amphipathic bile acid-conjugated polyethyleneimine

53. Medicinal plant transcriptomes: the new gateways for accelerated understanding of plant secondary metabolism

54. Non-viral gene activated matrices for mesenchymal stem cells based tissue engineering of bone and cartilage

55. Plant Based Bioreactors of Recombinant Cytokines (Review)

56. Short O-GalNAc glycans: regulation and role in tumor development and clinical perspectives

57. Biological and Biomedical Applications of Engineered Nucleases

58. Biotechnological aspects of cytoskeletal regulation in plants

59. Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

60. Electroporation-based applications in biotechnology

61. Engineering mesenchymal stem cells for regenerative medicine and drug delivery

62. Fermentative production of glycine betaine and trehalose from acid whey using Actinopolyspora halophila (MTCC 263)

63. Lost signature: progress and failures in in vivo tracking of implanted stem cells

64. MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via Cationized Pleurotus eryngii Polysaccharide-based Nanotransfection

65. Stress, adaptation, and speciation in the evolution of the blind mole rat, Spalax, in Israel

66. Development and Applications of CRISPR-Cas9 for Genome Engineering

67. Efficient Generation of Hepatic Cells from Multipotent Adult Mouse Germ-Line Stem Cells Using an OP9 Co-Culture System

68. Histone deacetylase 8 suppresses osteogenic differentiation of bone marrow stromal cells by inhibiting histone H3K9 acetylation and RUNX2 activity

69. Investigation of Phase Separation Behavior and Formation of Plasmonic Nanocomposites from Polypeptide-Gold Nanorod Nanoassemblies

70. Neurogenesis and vascularization of the damaged brain using a lactate-releasing biomimetic scaffold

71. Polymersome surface decoration by an EGFP fusion protein employing Cecropin A as peptide âanchorâ