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1. Transduction Enhancers Enable Efficient Human Adenovirus Type 5-Mediated Gene Transfer into Human Multipotent Mesenchymal Stromal Cells

Author:
Robin Nilson; Olivia Lübbers; Linus Weiß; Karmveer Singh; Karin Scharffetter-Kochanek; Markus Rojewski; Hubert Schrezenmeier; Philip Helge Zeplin; Wolfgang Funk; Lea Krutzke; Stefan Kochanek; Astrid Kritzinger
Source:
Viruses 2021 v.13 no.6 pp. -
ISSN:
1999-4915
Subject:
Human mastadenovirus C; blood coagulation; cancer therapy; capsid; gene transfer; genes; genetic engineering; humans; immunomodulation; lactoferrin; medicine; migratory behavior; spermidine; spermine; tumor necrosis factors
Abstract:
... Human multipotent mesenchymal stromal cells (hMSCs) are currently developed as cell therapeutics for different applications, including regenerative medicine, immune modulation, and cancer treatment. The biological properties of hMSCs can be further modulated by genetic engineering. Viral vectors based on human adenovirus type 5 (HAdV-5) belong to the most frequently used vector types for genetic m ...
DOI:
10.3390/v13061136

2. Biotechnology of camptothecin production in Nothapodytes nimmoniana, Ophiorrhiza sp. and Camptotheca acuminata

Author:
Mallappa Kumara Swamy; Shreya Nath; Subhabrata Paul; Niraj Kumar Jha; Boregowda Purushotham; Komdur Channabasavaraju Rohit; Abhijit Dey
Source:
Applied microbiology and biotechnology 2021 v.105 no.24 pp. 9089-9102
ISSN:
0175-7598
Subject:
Camptotheca acuminata; Icacinaceae; Ophiorrhiza; antineoplastic agents; bark; biosynthesis; biotechnology; cancer therapy; drug development; endangered species; ex situ conservation; genetic engineering; genetic variation; habitats; human diseases; humans; medicinal plants; medicine; microbiology; plant biochemistry; secondary metabolites; traditional farming
Abstract:
... Cancer is probably the deadliest human disease in recent years. In the past few years, rapid clinical progress has been made in the field of anticancer drug development. Plant secondary metabolites have been noted as extremely efficacious as promising natural source for anticancer therapy for many years. Camptothecin (CPT) is one of the popularly used anti-tumor drugs possessing clinically proven ...
DOI:
10.1007/s00253-021-11700-5

3. Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

Author:
Christoph Wawrosch; Sergey B. Zotchev
Source:
Applied microbiology and biotechnology 2021 v.105 no.18 pp. 6649-6668
ISSN:
0175-7598
Subject:
biochemical pathways; biotechnology; commercialization; cost effectiveness; genetic engineering; genetically modified organisms; humans; indigenous species; medicine; phytochemicals; raw materials; secondary metabolites; synthesis; tissue culture
Abstract:
... Medicinal plants have been used by mankind since ancient times, and many bioactive plant secondary metabolites are applied nowadays both directly as drugs, and as raw materials for semi-synthetic modifications. However, the structural complexity often thwarts cost-efficient chemical synthesis, and the usually low content in the native plant necessitates the processing of large amounts of field-cul ...
DOI:
10.1007/s00253-021-11539-w

4. Research advances on arachidonic acid production by fermentation and genetic modification of Mortierella alpina

Author:
Huidan Zhang; Qiu Cui; Xiaojin Song
Source:
World journal of microbiology & biotechnology 2021 v.37 no.1 pp. 4
ISSN:
0959-3993
Subject:
Mortierella alpina; arachidonic acid; biotechnology; cosmetics; culture media; fermentation; genetic engineering; humans; medicine; nutrition; synthetic biology
Abstract:
... Arachidonic acid (ARA, 5, 8, 11, 14-cis-eicosatetraenoic acid) is a relevant ω-6 polyunsaturated fatty acid, which plays essential roles in human immune, cardiovascular, and nervous systems. It is widely used in medicine, cosmetics, nutrition, and other fields. Traditionally, ARA is obtained from animal tissues. However, due to the limitation and unsustainability of existing resources, microorgani ...
DOI:
10.1007/s11274-020-02984-2

5. An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells

Author:
Krishna Kumar Haridhasapavalan; Manash P. Borgohain; Chandrima Dey; Bitan Saha; Gloria Narayan; Sachin Kumar; Rajkumar P. Thummer
Source:
Gene 2019 v.686 pp. 146-159
ISSN:
0378-1119
Subject:
induced pluripotent stem cells; risk; Murine respirovirus; medicine; humans; drug evaluation; ethics; somatic cells; plasmids; therapeutics; transfection; embryonic stem cells; genetic engineering; genomics; fibroblasts; Adenoviridae; transposons
Abstract:
... Over a decade ago, a landmark study that reported derivation of induced Pluripotent Stem Cells (iPSCs) by reprogramming fibroblasts has transformed stem cell research attracting the interest of the scientific community worldwide. These cells circumvent the ethical and immunological concerns associated with embryonic stem cells, and the limited self-renewal ability and restricted differentiation po ...
DOI:
10.1016/j.gene.2018.11.069

6. Genetic circuitry for personalized human cell therapy

Author:
Fabian Tolle; Pascal Stücheli; Martin Fussenegger
Source:
Current opinion in biotechnology 2019 v.59 pp. 31-38
ISSN:
0958-1669
Subject:
gene expression; gene regulatory networks; genetic engineering; humans; medical treatment; medicine; protein synthesis; synthetic biology; synthetic genes
Abstract:
... Synthetic biology uses engineering principles to design and assemble biological components and systems for a variety of applications. On the basis of genetic engineering, synthetic gene switches can be interconnected to construct complex gene circuits, capable of sensing and integrating diverse input signals for precise spatiotemporal control of target gene expression in living cells. Designer cel ...
DOI:
10.1016/j.copbio.2019.02.003

7. Genetically Engineered Adipose Mesenchymal Stem Cells Using HIV-Based Lentiviral Vectors as Gene Therapy for Autoimmune Diseases

Author:
Masoumeh Rostami; Kamran Haidari; Majid Shahbazi
Source:
Cellular reprogramming 2018 v.20 no.6 pp. 337-346
ISSN:
2152-4998
Subject:
CD4-positive T-lymphocytes; autoimmune diseases; bioactive properties; gene therapy; genetic engineering; humans; immunosuppression; inflammation; interleukin-10; interleukin-17; interleukin-23; medicine; mesenchymal stromal cells; mice; quantitative polymerase chain reaction; splenocytes; transcription factors
Abstract:
... The immunomodulatory and self-renewable features of human adipose-derived mesenchymal stem cells (hAD-MSCs) mark their importance in regenerative medicine. Interleukin (IL)-23 as a proinflammatory cytokine suppresses T regulatory cells and promotes the response of T helper 17 and T helper 1 cells. This pathway initiates inflammation and immunosuppression in several autoimmune diseases. The current ...
DOI:
10.1089/cell.2018.0006

8. Applications of genetically engineered human pluripotent stem cell reporters in cardiac stem cell biology

Author:
Joe Z Zhang; Hongchao Guo; Joseph C Wu
Source:
Current opinion in biotechnology 2018 v.52 pp. 66-73
ISSN:
0958-1669
Subject:
cardiovascular diseases; genetic engineering; humans; inheritance (genetics); medicine; stem cells; technology
Abstract:
... The advent of human pluripotent stem cells (hPSCs) has benefited many fields, from regenerative medicine to disease modeling, with an especially profound effect in cardiac research. Coupled with other novel technologies in genome engineering, hPSCs offer a great opportunity to delineate human cardiac lineages, investigate inherited cardiovascular diseases, and assess the safety and efficacy of cel ...
DOI:
10.1016/j.copbio.2018.03.002
PubMed:
29579626
PubMed Central:
PMC6082724

9. Biomaterials-based 3D cell printing for next-generation therapeutics and diagnostics

Author:
Jinah Jang; Ju Young Park; Ge Gao; Dong-Woo Cho
Source:
Biomaterials 2018 v.156 pp. 88-106
ISSN:
0142-9612
Subject:
biochemical compounds; biocompatible materials; bioprinting; diagnostic techniques; genetic engineering; humans; manufacturing; medicine; morphogenesis; polymerization; stem cells; therapeutics; tissue engineering; tissues
Abstract:
... Building human tissues via 3D cell printing technology has received particular attention due to its process flexibility and versatility. This technology enables the recapitulation of unique features of human tissues and the all-in-one manufacturing process through the design of smart and advanced biomaterials and proper polymerization techniques. For the optimal engineering of tissues, a higher-or ...
DOI:
10.1016/j.biomaterials.2017.11.030

10. Can Reprogramming of Overall Epigenetic Memory and Specific Parental Genomic Imprinting Memory within Donor Cell-Inherited Nuclear Genome be a Major Hindrance for the Somatic Cell Cloning of Mammals? – A Review

Author:
Marcin Samiec; Maria Skrzyszowska
Source:
Annals of animal science 2018 v.18 no.3 pp. 623-638
ISSN:
2300-8733
Subject:
DNA; acetylation; animal science; chromatin; cytosine; demethylation; early development; gene expression; genetic engineering; genomics; germ cells; histones; humans; lysine; medicine; memory; nuclear genome; somatic cells; transcription (genetics)
Abstract:
... Successful cloning of animals by somatic cell nuclear transfer (SCNT) requires epigenetic transcriptional reprogramming of the differentiated state of the donor cell nucleus to a totipotent embryonic ground state. It means that the donor nuclei must cease its own program of gene expression and restore a particular program of the embryonic genome expression regulation that is necessary for normal d ...
DOI:
10.2478/aoas-2018-0015

11. DNA Modulates the Interaction of Genetically Engineered DNA-Binding Proteins and Gold Nanoparticles: Diagnosis of High-Risk HPV Infection

Author:
Ju-Yi Mao; Han-Wei Li; Shih-Chun Wei; Scott G. Harroun; Ming-Ying Lee; Hung-Yun Lin; Chih-Yu Chung; Chun-Hua Hsu; Yet-Ran Chen; Han-Jia Lin; Chih-Ching Huang
Source:
ACS applied materials & interfaces 2017 v.9 no.51 pp. 44307-44315
ISSN:
1944-8252
Subject:
DNA; DNA-binding proteins; Papillomaviridae; cysteine; genes; genetic disorders; gold; humans; medicine; nanogold; polymerase chain reaction; protein engineering
Abstract:
... Gene detection has an important role in diagnosing several serious diseases and genetic defects in modern clinical medicine. Herein, we report a fast and convenient gene detection method based on the modulation of the interaction between a heat-resistant double-stranded DNA (dsDNA)-binding protein (Sso7d) and gold nanoparticles (Au NPs). We prepared a recombinant Cys-Sso7d, which is Sso7d with an ...
DOI:
10.1021/acsami.7b13873

12. Rational Design of Mouse Models for Cancer Research

Author:
Marietta Landgraf; Jacqui A. McGovern; Peter Friedl; Dietmar W. Hutmacher
Source:
Trends in biotechnology 2018 v.36 pp. 242-251
ISSN:
0167-7799
Subject:
animal models; genetic engineering; genomics; human diseases; humans; medicine; mice; neoplasms; patients; therapeutics; tissue engineering
Abstract:
... The laboratory mouse is widely considered as a valid and affordable model organism to study human disease. Attempts to improve the relevance of murine models for the investigation of human pathologies led to the development of various genetically engineered, xenograft and humanized mouse models. Nevertheless, most preclinical studies in mice suffer from insufficient predictive value when compared ...
DOI:
10.1016/j.tibtech.2017.12.001

13. Genetic and metabolic engineering of microorganisms for the development of new flavor compounds from terpenic substrates

Author:
Murillo L. Bution; Gustavo Molina; Meissa R.E. Abrahão; Gláucia M. Pastore
Source:
Critical reviews in biotechnology 2015 v.35 no.3 pp. 313-325
ISSN:
1549-7801
Subject:
biotechnology; cosmetics; drugs; flavor compounds; food additives; human nutrition; humans; industry; medicine; metabolic engineering; microorganisms; odors; synthetic biology; systems biology; terpenoids; therapeutics
Abstract:
... Throughout human history, natural products have been the basis for the discovery and development of therapeutics, cosmetic and food compounds used in industry. Many compounds found in natural organisms are rather difficult to chemically synthesize and to extract in large amounts, and in this respect, genetic and metabolic engineering are playing an increasingly important role in the production of ...
DOI:
10.3109/07388551.2013.855161

14. Recent developments and clinical studies utilizing engineered zinc finger nuclease technology

Author:
Young-Il Jo; Hyongbum Kim; Suresh Ramakrishna
Source:
Cellular and molecular life sciences 2015 v.72 no.20 pp. 3819-3830
ISSN:
1420-682X
Subject:
DNA; biotechnology; clinical trials; cold stress; genes; genetic disorders; genetic engineering; homologous recombination; humans; medicine; mutants; nucleases; zinc finger motif
Abstract:
... Efficient methods for creating targeted genetic modifications have long been sought for the investigation of gene function and the development of therapeutic modalities for various diseases, including genetic disorders. Although such modifications are possible using homologous recombination, the efficiency is extremely low. Zinc finger nucleases (ZFNs) are custom-designed artificial nucleases that ...
DOI:
10.1007/s00018-015-1956-5

15. MicroRNA Replacing Oncogenic Klf4 and c-Myc for Generating iPS Cells via Cationized Pleurotus eryngii Polysaccharide-based Nanotransfection

Author:
Deng Wenwen; Cao Xia; Chen Jingjing; Zhang Zhijian; Yu Qingtong; Wang Yan; Shao Genbao; Zhou Jie; Gao Xiangdong; Yu Jiangnan; Xu Ximing
Source:
ACS Applied Materials & Interfaces 2015 v.7 no.34 pp. 18957-18966
ISSN:
1944-8252
Subject:
Pleurotus eryngii; genetic engineering; humans; induced pluripotent stem cells; medicine; microRNA; nanoparticles; particle size; physicochemical properties; plasmids; polysaccharides; transcription factors; umbilical cord
Abstract:
... Induced pluripotent stem cells (iPSCs), resulting from the forced expression of cocktails out of transcription factors, such as Oct4, Sox2, Klf4, and c-Myc (OSKM), has shown tremendous potential in regenerative medicine. Although rapid progress has been made recently in the generation of iPSCs, the safety and efficiency remain key issues for further application. In this work, microRNA 302-367 was ...
DOI:
10.1021/acsami.5b06768

16. Efficient Generation of Hepatic Cells from Multipotent Adult Mouse Germ-Line Stem Cells Using an OP9 Co-Culture System

Author:
Streckfuss-Bömeke Katrin; Jende Jörg; Cheng I-Fen; Hasenfuss Gerd; Guan Kaomei
Source:
Cellular reprogramming 2014 v.16 no.1 pp. 65-76
ISSN:
2152-4998
Subject:
GATA transcription factors; adult stem cells; adults; albumins; coculture; embryonic stem cells; gelatin; genetic engineering; glycogen; hepatocytes; humans; liver; low density lipoprotein; medicine; mice; models; secretion; testes; urea
Abstract:
... On the basis of their self-renewal capacity and their ability to differentiate into derivatives of all three germ layers, germ line–derived multipotent adult stem cells (maGSCs) from mouse testis might serve as one of preferable sources for pluripotent stem cells in regenerative medicine. In our study, we aimed for an efficient hepatic differentiation protocol that is applicable for both maGSCs an ...
DOI:
10.1089/cell.2013.0057
PubMed:
24380658
PubMed Central:
PMC3920844

17. New Tool for Genome Surgery

Author:
John van der Oost
Source:
Science 2013 v.339 no.6121 pp. 768-770
ISSN:
0036-8075
Subject:
DNA; binding sites; gene therapy; genes; genetic engineering; humans; medicine; mutation; nucleases; nucleotide sequences; patients
Abstract:
... Gene therapy is the holy grail of human medicine. Many diseases are caused by a defective gene, sometimes with a mutation as subtle as a single-nucleotide variation. Before restoration of such a mutation in a patient's genome can take place, the target nucleotide sequence has to be cleaved at a single position, out of 3 billion possibilities. This degree of precise surgery requires an enzyme with ...
DOI:
10.1126/science.1234726

18. Technical advances to genetically engineering human embryonic stem cells

Author:
Julien Jean Pierre Maury; Andre Boon-Hwa Choo; Ken Kwok-Keung Chan
Source:
Integrative biology 2011 v.3 no.7 pp. 717-723
ISSN:
1757-9708
Subject:
DNA; Lentivirus; biomedical research; embryonic stem cells; genes; genetic engineering; human development; humans; medicine; models; nucleases; nucleotide sequences; therapeutics; transposases; zinc finger motif
Abstract:
... Human embryonic stem cells (hESC) are important to basic scientific research as an in vitro model system for the study of human development and to clinical research as an invaluable cell source for regenerative medicine. The ability to genetically engineer hESC is a critical resource as it facilitates many fundamental studies to understand gene regulation and cell development. These techniques inc ...
DOI:
10.1039/c1ib00019e

19. Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease

Author:
Grizot, Sylvestre; Smith, Julianne; Daboussi, Fayza; Prieto, Jesús; Redondo, Pilar; Merino, Nekane; Villate, Maider; Thomas, Séverine; Lemaire, Laetitia; Montoya, Guillermo; Blanco, Francisco J.; Pâques, Frédéric; Duchateau, Philippe
Source:
Nucleic acids research 2009 v.37 no.16 pp. 5405-5419
ISSN:
0305-1048
Subject:
chemical elements; engineering; gene therapy; genes; genetic engineering; genotoxicity; humans; loci; medicine; mutation; nucleic acids; severe combined immunodeficiency; viruses
Abstract:
... Sequence-specific endonucleases recognizing long target sequences are emerging as powerful tools for genome engineering. These endonucleases could be used to correct deleterious mutations or to inactivate viruses, in a new approach to molecular medicine. However, such applications are highly demanding in terms of safety. Mutations in the human RAG1 gene cause severe combined immunodeficiency (SCID ...
DOI:
10.1093/nar/gkp548
PubMed:
19584299
PubMed Central:
PMC2760784

20. Highly efficient transient gene expression and gene targeting in primate embryonic stem cells with helper-dependent adenoviral vectors

Author:
Suzuki, Keiichiro; Mitsui, Kaoru; Aizawa, Emi; Hasegawa, Kouichi; Kawase, Eihachiro; Yamagishi, Toshiyuki; Shimizu, Yoshihiko; Suemori, Hirofumi; Nakatsuji, Norio; Mitani, Kohnosuke
Source:
Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.37 pp. 13781-13786
ISSN:
0027-8424
Subject:
Macaca fascicularis; clinical trials; embryonic stem cells; gene expression; gene targeting; gene transfer; genes; genetic engineering; homologous recombination; humans; medicine; mice
Abstract:
... Human embryonic stem (hES) cells are regarded as a potentially unlimited source of cellular materials for regenerative medicine. For biological studies and clinical applications using primate ES cells, the development of a general strategy to obtain efficient gene delivery and genetic manipulation, especially gene targeting via homologous recombination (HR), would be of paramount importance. Howev ...
DOI:
10.1073/pnas.0806976105
PubMed:
18768795
PubMed Central:
PMC2544531