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You searched for: Subject messenger RNA Remove constraint Subject: messenger RNA Subject term DNA-directed RNA polymerase Remove constraint Subject term: DNA-directed RNA polymerase Subject term DNA microarrays Remove constraint Subject term: DNA microarrays
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1. Improving Saccharomyces cerevisiae ethanol production and tolerance via RNA polymerase II subunit Rpb7

Author:
Zilong Qiu; Rongrong Jiang
Source:
Biotechnology for biofuels 2017 v.10 no.1 pp. 125
ISSN:
1754-6834
Subject:
DNA microarrays; DNA-directed RNA polymerase; Saccharomyces cerevisiae; alcoholic fermentation; directed evolution; engineering; ethanol; ethanol production; gene expression regulation; genes; glycolysis; messenger RNA; oxidative stress; phenotype; polymerase chain reaction; stress response; transcription (genetics); yeasts
Abstract:
... BACKGROUND: Classical strain engineering methods often have limitations in altering multigenetic cellular phenotypes. Here we try to improve Saccharomyces cerevisiae ethanol tolerance and productivity by reprogramming its transcription profile through rewiring its key transcription component RNA polymerase II (RNAP II), which plays a central role in synthesizing mRNAs. This is the first report on ...
DOI:
10.1186/s13068-017-0806-0
PubMed:
28515784
PubMed Central:
PMC5433082

2. On-Chip Synthesis of Protein Microarrays from DNA Microarrays via Coupled In Vitro Transcription and Translation for Surface Plasmon Resonance Imaging Biosensor Applications

Author:
Seefeld Ting H.; Halpern Aaron R.; Corn Robert M.
Source:
Journal of the American Chemical Society 2012 v.134 no.30 pp. 12358-12361
ISSN:
1520-5126
Subject:
green fluorescent protein; image analysis; messenger RNA; translation (genetics); copper; antibodies; DNA; luciferase; DNA microarrays; transcription (genetics); cell-free protein synthesis; surface plasmon resonance; DNA-directed RNA polymerase; biosensors; adsorption
Abstract:
... Protein microarrays are fabricated from double-stranded DNA (dsDNA) microarrays by a one-step, multiplexed enzymatic synthesis in an on-chip microfluidic format and then employed for antibody biosensing measurements with surface plasmon resonance imaging (SPRI). A microarray of dsDNA elements (denoted as generator elements) that encode either a His-tagged green fluorescent protein (GFP) or a His-t ...
DOI:
10.1021/ja304187r
PubMed:
22793370
PubMed Central:
PMC3415242

3. Global gene expression analysis of Aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia

Author:
Masuo, Shunsuke; Terabayashi, Yasunobu; Shimizu, Motoyuki; Fujii, Tatsuya; Kitazume, Tatsuya; Takaya, Naoki
Source:
Molecular genetics and genomics 2010 v.284 no.6 pp. 415-424
ISSN:
1617-4615
Subject:
Aspergillus nidulans; DNA microarrays; DNA-directed RNA polymerase; NAD (coenzyme); aeration; anaerobic conditions; ethanol; ethanol production; fungi; gamma-aminobutyric acid; gene expression; gene expression regulation; genes; glucose; glycolysis; hypoxia; messenger RNA; normoxia; oxygen; transcription (genetics); tricarboxylic acid cycle
Abstract:
... Hypoxia imposes a challenge upon most filamentous fungi that require oxygen for proliferation. Here, we used whole genome DNA microarrays to investigate global transcriptional changes in Aspergillus nidulans gene expression after exposure to hypoxia followed by normoxia. Aeration affected the expression of 2,864 genes (27% of the total number of genes in the fungus), of which 50% were either induc ...
DOI:
10.1007/s00438-010-0576-x

4. Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification

Author:
Tougan, Takahiro; Okuzaki, Daisuke; Nojima, Hiroshi
Source:
Nucleic acids research 2008 v.36 no.15 pp. e92
ISSN:
0305-1048
Subject:
DNA microarrays; DNA-directed RNA polymerase; cDNA libraries; chemical elements; complementary DNA; mammals; messenger RNA; polymerase chain reaction
Abstract:
... Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is still required for cDNA library preparation from single-cell quantities of RNA. We have designed a small RNA molecule called chum-RNA that has enabled us to prepare a single-cell cDNA library after four r ...
DOI:
10.1093/nar/gkn420
PubMed:
18603591
PubMed Central:
PMC2528176

5. The role of the omega subunit of RNA polymerase in expression of the relA gene in Escherichia coli

Author:
Chatterji, Dipankar; Ogawa, Yoshito; Shimada, Tomohiro; Ishihama, Akira
Source:
FEMS microbiology letters 2007 v.267 no.1 pp. 51-55
ISSN:
0378-1097
Subject:
messenger RNA; starvation; stress response; DNA microarrays; gene expression; ribosomal proteins; operon; DNA-directed RNA polymerase; Escherichia coli
Abstract:
... The rpoZ gene for the omega subunit of Escherichia coli RNA polymerase constitutes single operon with the spoT gene, which is responsible for the maintenance of stringent response under nutrient starvation conditions. To identify the physiological role of the omega subunit, we compared the gene expression profile of wild-type Escherichia coli with that of an rpoZ deleted strain by microarray analy ...
DOI:
10.1111/j.1574-6968.2006.00532.x