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You searched for: Subject pyridoxal phosphate Remove constraint Subject: pyridoxal phosphate Subject term pyridoxal phosphate Remove constraint Subject term: pyridoxal phosphate Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. Degradation and binding to food proteins of vitamin B-6 compounds during thermal processing

Author:
Gregory, J.F. III.; Ink, S.L.; Sartain, D.B.
Source:
Journal of food science 1986 v.51 no.5 pp. 1345-1351
ISSN:
0022-1147
Subject:
food processing; heat treatment; pyridoxine; pyridoxal; pyridoxal phosphate; pyridoxamine; degradation; proteins
DOI:
10.1111/j.1365-2621.1986.tb13119.x

2. Mechanistic deductions from kinetic isotope effects and pH studies of pyridoxal phosphate dependent carbon-carbon lyases: Erwinia herbicola and Citrobacter freundii tyrosine phenol-lyase

Author:
Kiick, D.M.; Phillips, R.S.
Source:
Biochemistry 1988 v.27 no.19 pp. 7333-7338
ISSN:
0006-2960
Subject:
Pantoea agglomerans; Citrobacter freundii; pH; pyridoxal phosphate; carbon; lyases; kinetics; tyrosine
DOI:
10.1021/bi00419a023
PubMed:
3207679

3. Inactivation of chloroplast H+-ATPase by modification of Lysbeta 359, Lysalpha 176 and Lysalpha 266

Author:
Horbach, M.; Meyer, H.E.; Bickel-Sandkotter, S.
Source:
European journal of biochemistry 1991 v.200 no.2 pp. 449-456
ISSN:
0014-2956
Subject:
chloroplasts; adenosinetriphosphatase; pyridoxal phosphate; magnesium; lysine; enzyme activity; inactivation; molecular conformation; protein structure; binding sites
Abstract:
... Treatment of isolated, latent chloroplast ATPase with pyridoxal-5-phosphate (pyridoxal-P) in presence of Mg2+ causes inhibition of dithiothreitol-activated plus heat-activated ATP hydrolysis. The amount of [3H]pyridoxal-P bound to chloroplast coupling factor 1 (CF1) was estimated to run up to 6 +/- 1 pyridoxal-P/enzyme, almost equally distributed between the alpha- and beta-subunits. Inactivation, ...
DOI:
10.1111/j.1432-1033.1991.tb16203.x
PubMed:
1832378

4. Transport processes and corresponding changes in metabolite levels in relation to starch synthesis in barley (Hordeum vulgare L.) etioplasts

Author:
Batz, O.; Scheibe, R.; Neuhaus, H.E.; Transport processes and corresponding changes in metabolite levels in relation to starch synthesis in barley (Hordeum vulgare L.) etioplasts.
Source:
Plant physiology 1992 v.100 no.1 pp. 184-190
ISSN:
0032-0889
Subject:
Hordeum vulgare; starch; carbohydrate metabolism; etioplasts; adenosine triphosphate; pyridoxal phosphate; glucose 6-phosphate; active transport; enzymes; enzyme activity; inorganic phosphorus
Abstract:
... Intact etioplasts with an intactness of 85% and with a cytosolic and a mitochondrial contamination of less than 10% were isolated from 8-d-old dark-grown barley (Hordeum vulgare) leaves. These plastids contained starch equivalent to 21.5 micromoles of glucose per mg protein. From various likely precursors applied to isolated etioplasts, only dihydroxyacetone phosphate (DHAP) had significant effect ...
DOI:
10.1104/pp.100.1.184
PubMed:
16652944

5. A S-cysteine conjugate, precursor of aroma of White Sauvignon

Author:
Takatoshi Tominaga; Isabelle Masneuf-Pomarède; Denis Dubourdieu
Source:
OENO One 1995 v.29 no.4 pp. 227-232
ISSN:
2494-1271
Subject:
Eubacterium limosum; acetates; ammonium; bacteria; cysteine; enzymes; gastrointestinal system; glutathione; odor compounds; odors; pyridoxal phosphate; pyruvic acid
Abstract:
... 4-mercapto-4-methylpentan-2-one (4-MMP), a strongly odorant compound responsible for the « boxtree » or « broom plant » odour of the Sauvignon wines, can be enzymaticaly released in vitro from an odourless must extract. The enzyme source used is a cell-free extract of the gastrointestinal bacterium Eubacterium limosum. This crude preparation exhibits a cysteine β-lyase activity which requires the ...
DOI:
10.20870/oeno-one.1995.29.4.1123

6. Mice lacking tissue non-specific alkaline phosphatase die from seizures due to defective metabolism of vitamin B-6

Author:
Waymire, K.G.; Mahuren, J.D.; Jaje, J.M.; Guilarte, T.R.; Coburn, S.P.; MacGregor, G.R.
Source:
Nature genetics 1995 v.11 no.1 pp. 45-51
ISSN:
1061-4036
Subject:
mice; pyridoxal phosphate; pyridoxine; metabolism; metabolic diseases; alkaline phosphatase; enzyme deficiencies; teeth; structural genes; homologous recombination; nervous system diseases; gamma-aminobutyric acid; brain
DOI:
10.1038/ng0995-45
PubMed:
7550313

7. Energizing effects of illumination on the reactivities of lysine residues of the gamma-subunit of chloroplast ATP synthase

Author:
Komatsu-Takaki, M.
Source:
European journal of biochemistry 1996 v.236 no.2 pp. 470-475
ISSN:
0014-2956
Subject:
H+/K+-exchanging ATPase; H-transporting ATP synthase; ammonium chloride; electrochemistry; lighting; lysine; protons; pyridoxal phosphate; thylakoids
Abstract:
... Incubation of chloroplast thylakoids with pyridoxal 5'-phosphate for a short time (5 s) modified the lysine residues of the gamma subunit of ATP synthase. Energization of thylakoids by illumination increased the reactivity of Lys24 by a factor of three and decreased the reactivity of Lys30 to 60%. The reactivities of these residues reached their maximum and minimum values, respectively, within 1 s ...
DOI:
10.1111/j.1432-1033.1996.00470.x
PubMed:
8612618

8. Spinach chloroplast O-acetylserine (thiol)-lyase exhibits two catalytically non-equivalent pyridoxal-5'-phosphate-containing active sites

Author:
Rolland, N.; Ruffet, M.L.; Job, D.; Douce, R.; Droux, M.
Source:
European journal of biochemistry 1996 v.236 no.1 pp. 272-282
ISSN:
0014-2956
Subject:
DNA-directed RNA polymerase; Escherichia coli; absorbance; acrylamides; active sites; affinity chromatography; agarose; cell culture; chloroplasts; fluorescence; iodides; nitrobenzoic acids; physical properties; pyridoxal phosphate; quantitative analysis; recombinant proteins; schiff bases; spinach; synthetic genes; thiols; tryptophan
Abstract:
... A synthetic gene encoding the mature spinach-chloroplast O-acetylserine (thiol)-lyase was constructed and expressed in an Escherichia coli strain carrying the T7 RNA polymerase system. The pure recombinant protein was obtained at high yield (6 mg/l cell culture) using a new purification procedure that includes affinity chromatography on Green A agarose. Its specific activity was of the order of 10 ...
DOI:
10.1111/j.1432-1033.1996.00272.x

9. Cysteine-S-conjugate β-Lyase Activity and Pyridoxal Phosphate Binding Site of Onion Alliin Lyase

Author:
Nobuko Kitamura; Naoya Shimomura; Junji Iseki; Mamoru Honma; Seiya Chiba; Satoshi Tahara; Junya Mizutani
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.8 pp. 1327-1330
ISSN:
1347-6947
Subject:
Mucor; allicin; alliin lyase; amino acid sequences; biotechnology; catalytic activity; cation exchange chromatography; lysine; onions; pyridoxal phosphate; sulfoxides
Abstract:
... Purification of onion alliin lyase gave two fractions by cation exchange chromatography. Both fractions showed the comparable high catalytic activity of cysteine-S-conjugate β-Iyase with that of alliin lyase using S-(2-chloro-6-nitrophenyl)-l-cysteine and alliin, S-allyl-l-cysteine sulfoxide as substrates. All the active substrates tested with onion alliin lyase were also active to the cysteine-S- ...
DOI:
10.1271/bbb.61.1327

10. Partial purification and characterisation of an aromatic amino acid aminotransferase from mung bean (Vigna radiata L. Wilczek)

Author:
Simpson, R.M.; Nonhebel, H.M.; Christie, D.L.
Source:
Planta 1997 v.201 no.1 pp. 71-77
ISSN:
0032-0935
Subject:
pyridoxal phosphate; molecular weight; transaminases; aspartic acid; Vigna radiata; tryptophan; keto acids; indole acetic acid; naphthaleneacetic acid; chemical constituents of plants; enzyme activity; amino acid metabolism; leaves; shoots
Abstract:
... An aromatic amino acid aminotransferase (aromAT) was purified over 33 000-fold from the shoots and primary leaves of mung beans (Vigna radiata L. Wilczek). The enzyme was purified by ammonium sulfate precipitation, gel filtration and anion exchange followed by fast protein liquid chromatography using Mono Q and Phenylsuperose. The relative aminotransferase activities using the most active amino ac ...
DOI:
10.1007/BF01258682

11. Substitutions of Alanine for Cysteine at a Reactive Thiol Site and for Lysine at a Pyridoxal Phosphate Binding Site of 1-Aminocyclopropane-1-carboxylate Deaminase

Author:
Toyotaka Murakami; Miwa Kiuchi; Hiroyuki Ito; Hirokazu Matsui; Mamoru Honma
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.3 pp. 506-509
ISSN:
1347-6947
Subject:
1-aminocyclopropane-1-carboxylate deaminase; 1-aminocyclopropane-1-carboxylic acid; absorption; alanine; aldimines; biotechnology; catalytic activity; cysteine; deamination; enzyme activity; lysine; mutants; pyridoxal phosphate; serine; thiols; valine
Abstract:
... 1-Aminocyclopropane-1-carboxylate (ACC) deaminase catalyzes the cyclopropane ring fragmentation and deamination of ACC. Replacement of cysteine with alanine at a reactive thiol site, Cys-162, of ACC deaminase did not affect the enzyme activity, in spite of the previous result that modification of Cys-162 caused complete loss of the enzyme activity. Substitution of glycine or valine for the cystein ...
DOI:
10.1271/bbb.61.506

12. Differential hydrolysis of alk(en)yl cysteine sulphoxides by alliinase in onion macerates: flavour implications

Author:
Lancaster, J.E.; Shaw, M.L.; Randle, W.M.
Source:
Journal of the science of food and agriculture 1998 v.78 no.3 pp. 367-372
ISSN:
0022-5142
Subject:
onions; mechanical damage; organic sulfur compounds; hydrolysis; enzyme activity; lyases; pyruvic acid; peptides; cultivars; Allium cepa; sulfur; nutrient availability; pyridoxal phosphate
Abstract:
... Intact cells of Allium spp have no odour, but when cells are disrupted, the enzyme alliinase (EC 4.4.1.4) hydrolyses the S-alk(en)yl sulphoxides (ACSOs) to produce pyruvate, ammonia and the many volatile sulphur compounds associated with flavour and odour. In onions, there are three main sulphoxides: methyl, propyl and 1-propenyl, which gives rise to onion's tear-producing effect. The relative pro ...
DOI:
10.1002/(SICI)1097-0010(199811)78:3<367::AID-JSFA127>3.0.CO;2-N

13. Purification and Characterization of Trehalose Phosphorylase from Catellatospora ferruginea

Author:
Kazuo AISAKA; Tomomi MASUDA; Tadashi CHIKAMUNE; Kazuyo KAMITORI
Source:
Bioscience, biotechnology, and biochemistry 1998 v.62 no.4 pp. 782-787
ISSN:
1347-6947
Subject:
Catellatospora ferruginea; ammonium chloride; biotechnology; gel chromatography; glucose; heat; heat stability; lithium chloride; molecular weight; phosphates; phosphorylase; polyacrylamide gel electrophoresis; pyridoxal phosphate; trehalose; xylose
Abstract:
... Trehalose phosphorylase was purified from the cell extracts of Catellatospora ferruginea. The enzyme had an apparent molecular weight of 400,000 by gel filtration and 98,000 by SDS-PAGE, suggesting that the enzyme was a tetramer. The enzyme was specific for trehalose in phosphorolysis and specific for β-D-glucose 1-phosphate in synthesis. In addition to D-glucose, D-xylose and D-fucose were also p ...
DOI:
10.1271/bbb.62.782

14. Guanidine hydrochloride-induced reversible unfolding of sheep liver serine hydroxymethyltransferase

Author:
B. Venkatesha; Jayant B. Udgaonkar; N. Appaji Rao; H. S. Savithri
Source:
Journal of biosciences 1999 v.24 no.1 pp. 69-77
ISSN:
0250-5991
Subject:
active sites; apoproteins; detergents; gel chromatography; glycine hydroxymethyltransferase; liver; polyethylene glycol; pyridoxal phosphate; sheep
Abstract:
... Equilibrium unfolding studies of sheep liver tetrameric serine hydroxymethyltransferase (SHMT, EC 2.1.2.1) revealed that the enzyme assumed apparent random coil structure above 3 M guanidine hydrochloride (GdnHCl). In the presence of non-ionic detergent Brij-35 and polyethylene glycol, the 6 M GdnHCI unfolded enzyme could be completely (> 95%) refolded by a 40-fold dilution. The refolded enzyme wa ...
DOI:
10.1007/BF02941109

15. Relationship between cigarette smoking and nutrient intakes and blood status indices of older people living in the UK: further analysis of data from the National Diet and Nutrition Survey of people aged 65 years and over, 1994/95

Author:
Walmsley, C.M.; Bates, C.J.; Pentice, A.; Cole, T.J.
Source:
Public health nutrition 1999 v.2 no.2 pp. 199-208
ISSN:
1368-9800
Subject:
nutrient intake; tocopherols; elderly; food intake; nutritional status; blood serum; men; women; pyridoxal phosphate; smoking (habit); erythrocytes; carotenoids; oxidation; folic acid; nutrients; dietary surveys; antioxidants; ascorbic acid; United Kingdom
PubMed:
10447248

16. Functional characterization and mechanism of action of recombinant human kynurenine 3‐hydroxylase

Author:
Jérôme Breton; Nilla Avanzi; Simona Magagnin; Nevie Covini; Giovanni Magistrelli; Liviana Cozzi; Antonella Isacchi
Source:
European journal of biochemistry 2000 v.267 no.4 pp. 1092-1099
ISSN:
0014-2956
Subject:
NAD (coenzyme); NADP (coenzyme); chlorides; glutathione transferase; humans; hydroxylation; kynurenine; mechanism of action; mitochondrial membrane; neurotoxicity; oxidation; pH; pyridoxal phosphate; recombinant proteins; tryptophan
Abstract:
... The mitochondrial outer membrane enzyme kynurenine 3‐hydroxylase (K3H) is an NADPH‐dependent flavin mono‐oxygenase involved in the tryptophan pathway, where it catalyzes the hydroxylation of kynurenine. K3H was transiently expressed in COS‐1 cells as a glutathione S‐transferase (GST) fusion protein, and the pure recombinant protein (rec‐K3H) was obtained with a specific activity of about 2000 nmol ...
DOI:
10.1046/j.1432-1327.2000.01104.x

17. Pyridoxal 5′‐phoshate Schiff base in Citrobacter freundii tyrosinephenol‐lyase: Ionic and tautomeric equilibria

Author:
Natalia P. Bazhulina; Yurii V. Morozov; Anastasia I. Papisova; Tatyana V. Demidkina
Source:
European journal of biochemistry 2000 v.267 no.6 pp. 1830-1836
ISSN:
0014-2956
Subject:
Citrobacter freundii; absorption; lognormal distribution; nitrogen; pH; potassium; pyridoxal; pyridoxal phosphate; schiff bases; sodium; spectral analysis; tyrosine
Abstract:
... Spectral properties of the internal Schiff base in tyrosine phenol‐lyase have been investigated in the presence of an activating cation K+ and a cation‐inhibitor Na+. The holoenzyme absorption spectra in the pH range 6.5–8.7 were recorded in the presence of K+. No apparent pKa value of the coenzyme chromophore was found in this pH range, indicating that the internal Schiff base does not chan ...
DOI:
10.1046/j.1432-1327.2000.01185.x

18. Effect of phosphate on stability of pyridoxal in the presence of lysine

Author:
Huang, T.C.; Chen, M.H.; Ho, C.T.
Source:
Journal of agricultural and food chemistry 2001 v.49 no.3 pp. 1559-1563
ISSN:
0021-8561
Subject:
lysine; pyridoxal phosphate; chemical reactions; amino acid derivatives
Abstract:
... The stability of the biologically active compound vitamin B6 in aqueous solution was investigated. Schiff base formation is the major reaction between the epsilon-amino group of lysine and the aldehyde group of both pyridoxal and pyridoxal phosphate. Model systems composed of equal molar concentrations of lysine with either pyridoxal or pyridoxal phosphate were used to study the effect of proton t ...
DOI:
10.1021/jf000886q
PubMed:
11312896

19. Identification of a critical lysine residue at the active site in glyceraldehyde‐3‐phosphate dehydrogenase of Ehrlich ascites carcinoma cell : Comparison with the rabbit muscle enzyme

Author:
Swapna Ghosh; Kasturi Mukherjee; Manju Ray; Subhankar Ray
Source:
European journal of biochemistry 2001 v.268 no.23 pp. 6037-6044
ISSN:
0014-2956
Subject:
NAD (coenzyme); active sites; ascites; carcinoma; catalytic activity; cysteine; dithiothreitol; glyceraldehyde-3-phosphate dehydrogenase; glycolysis; lysine; muscles; nitrobenzoic acids; pyridoxal phosphate; rabbits
Abstract:
... The involvement of the lysine residue present at the active site of Ehrlich ascites carcinoma (EAC) cell glyceraldehyde‐3‐phosphate dehydrogenase (Gra3PDH) was investigated by using the lysine specific reagents trinitrobenzenesulfonic acid (TNBS) and pyridoxal phosphate (PP). Both TNBS and PP inactivated EAC cell Gra3PDH with pseudo‐first‐order kinetics with the rate dependent on modifier concentr ...
DOI:
10.1046/j.0014-2956.2001.02522.x

20. Production of Pyridoxal Phosphate by a Mutant Strain of Schizosaccharomyces pombe

Author:
Ruamsub CHUMNANTANA; Kumi HIROSE; Hiromichi BABA; Toshiharu YAGI
Source:
Bioscience, biotechnology, and biochemistry 2001 v.65 no.8 pp. 1789-1795
ISSN:
1347-6947
Subject:
Schizosaccharomyces pombe; air; ammonium sulfate; biotechnology; culture media; cycloheximide; glucose; leucine; menadione; mutants; oxidative stress; plumbagin; pyridoxal phosphate; pyridoxine; superoxide anion
Abstract:
... Conditions for extracellular production of vitamin B₆ compounds (B₆), especially pyridoxal 5'-phosphate (PLP) by Schizosaccharomyces pombe leu1 strain were examined. The productivity was dependent on concentration of L-leucine in the culture medium: 30 mg/l gave the highest concentrations of total B₆ and PLP. The viable cells harvested at different growth phases showed different productivity: midd ...
DOI:
10.1271/bbb.65.1789

21. The biosynthesis of ovothiol A (N1‐methyl‐4‐mercaptohistidine) : Identification of S‐(4′‐l‐histidyl)‐l‐cysteine sulfoxide as an intermediate and the products of the sulfoxide lyase reaction

Author:
Ryan N. Vogt; Hendrik S. C. Spies; Daniel J. Steenkamp
Source:
European journal of biochemistry 2001 v.268 no.20 pp. 5229-5241
ISSN:
0014-2956
Subject:
Crithidia fasciculata; NAD (coenzyme); NADH dehydrogenase; ammonium sulfate; anion exchange chromatography; biosynthesis; cysteine; dithiothreitol; high performance liquid chromatography; histidine; iron; isotope labeling; lactate dehydrogenase; oxygen; pyridoxal phosphate; pyruvic acid; spectral analysis; spectroscopy; sulfur; thiols
Abstract:
... Crude extracts of Crithidia fasciculata catalyse the formation of 4‐mercapto‐l‐histidine, an intermediate in the biosynthesis of ovothiol A (N1‐methyl‐4‐mercaptohistidine), in the presence of histidine, cysteine, Fe2+ and pyridoxal phosphate. This activity was present in a 35–55% ammonium sulfate fraction that was shown to produce a transsulfuration intermediate in the absence of pyr ...
DOI:
10.1046/j.0014-2956.2001.02444.x

22. Determination of Vitamin B-6 Estimated Average Requirement and Recommended Dietary Allowance for Children Aged 7–12 Years Using Vitamin B-6 Intake, Nutritional Status and Anthropometry

Author:
Chang, Sue-Joan; Huang, Yi-Chia; Hsiao, Li-Ju; Lee, Yi-Chun; Hsuen, Shou-Ying
Source:
Journal of nutrition 2002 v.132 no.10 pp. 3130-3134
ISSN:
0022-3166
Subject:
4-pyridoxic acid; Estimated Average Requirement; adults; alanine transaminase; anthropometric measurements; boys; children; erythrocytes; girls; nutritional status; pyridoxal phosphate; pyridoxine; surveys; urine; Taiwan
Abstract:
... The Estimated Average Requirement (EAR) and Recommended Dietary Allowance (RDA) of vitamin B-6 for children were recently estimated by extrapolating from adult values because of limited available information. To determine vitamin B-6 requirements and provide recommendations for intakes, vitamin B-6 intake, nutritional status and anthropometry of 168 healthy children (79 boys and 89 girls) were stu ...
PubMed:
12368406

23. Formation of methional by Lactococcus lactis IFPL730 under cheese model conditions

Author:
Felix Amárita; Carmen M. Martínez-Cuesta; Gonzalo Taborda; Pilar L. Soto-Yárritu; Teresa Requena; Carmen Peláez
Source:
European food research & technology 2002 v.214 no.1 pp. 58-62
ISSN:
1438-2377
Subject:
Lactococcus lactis; cheeses; ewes; flavor; gluconolactone; methionine; odors; pasteurization; pyridoxal phosphate; sensory evaluation; skim milk; slurries; sodium chloride; sulfur; thiamin
Abstract:
... The present work describes the capacity of Lactococcus lactis to produce methional and other sulphur compounds derived from methionine (Met) in a cheese model system. Cheese slurries were prepared from pasteurized ewes' skimmed milk, chemically acidified with glucono-δ-lactone and homogenized aseptically adding Met, α-ketoglutarate, pyridoxal 5'-phosphate, thiamine pyrophosphate and NaCl. Slurries ...
DOI:
10.1007/s00217-001-0420-y

24. Gene Cloning, Purification, and Characterization of 2,3-Diaminopropionate Ammonia-lyase from Escherichia coli

Author:
Takuma UO; Tohru YOSHIMURA; Tozo NISHIYAMA; Nobuyoshi ESAKI
Source:
Bioscience, biotechnology, and biochemistry 2002 v.66 no.12 pp. 2639-2644
ISSN:
1347-6947
Subject:
Escherichia coli; Salmonella Typhimurium; active sites; biotechnology; catalytic activity; genes; mammals; molecular weight; pyridoxal; pyridoxal phosphate; sequence homology; serine; serine dehydratase; serine racemase; stereochemistry; threonine
Abstract:
... 2,3-Diaminopropionate ammonia-lyase (DAPAL), which catalyzes α,β-elimination of 2,3-diaminopropionate regardless of its stereochemistry, was purified from Salmonella typhimurium. We cloned the Escherichia coli ygeX gene encoding a putative DAPAL and purified the gene product to homogeneity. The protein obtained contained pyridoxal 5′-phosphate and was composed of two identical subunits with a calc ...
DOI:
10.1271/bbb.66.2639

25. Oral contraceptives and nutritional status in adolescent British girls

Author:
Thane, Christopher W.; Bates, Christopher J.; Prentice, Ann
Source:
Nutrition research 2002 v.22 no.4 pp. 449-462
ISSN:
0271-5317
Subject:
adolescents; alpha-tocopherol; ascorbic acid; beta-carotene; blood serum; body mass index; dietary surveys; elderly; gamma-tocopherol; girls; high density lipoprotein cholesterol; low density lipoprotein cholesterol; nutritional status; oral contraceptives; pyridoxal phosphate; triacylglycerols; vitamin A; vitamin B12; zinc
Abstract:
... Associations between oral contraceptive pill (OC) use and vitamin, mineral and lipid status were examined in 117 postmenarcheal 16-to-18-year-old British girls, from the 1997 National Diet and Nutrition Survey of young people aged 4 to 18 years. Thirty-nine were taking OCs at the time of blood, anthropometric and sociodemographic assessment, while seventy-eight were not. After adjusting for age, b ...

26. Maternal periconceptional biochemical and hematological parameters, vitamin profiles and pregnancy outcome

Author:
Weerd, S. de; Steegers-Theunissen, R.P.M.; Boo, T.M. de; Thomas, C.M.G.; Steegers, E.A.P.
Source:
European journal of clinical nutrition 2003 v.57 no.9 pp. 1128-1134
ISSN:
0954-3007
Subject:
amenorrhea; birth weight; blood sampling; blood serum; embryogenesis; ferritin; folic acid; hematocrit; hemoglobin; hospitals; iron; longitudinal studies; pregnancy outcome; protein content; pyridoxal phosphate; riboflavin; risk; thiamin; uric acid; vitamin A; vitamin B12; women; Netherlands
Abstract:
... Objectives: To evaluate periconceptional maternal biochemical and hematological parameters and vitamin profiles in relation to the risk of early pregnancy loss and birth weight. Design: Prospective longitudinal study. Setting: University Medical Centre Nijmegen, Academic Medical Centre, Amsterdam, Maria and Elisabeth Hospitals, Tilburg, and Catharina Hospital, Eindhoven, The Netherlands. Subjects: ...
DOI:
10.1038/sj.ejcn.1601654

27. Some Properties of Glycine Aminotransferase Purified from Rhodopseudomonas palustris No. 7 Concerning Extracellular Porphyrin Production

Author:
Hidetoshi YAMAGUCHI; Masahiro OHTANI; Seigo AMACHI; Hirofumi SHINOYAMA; Takaaki FUJII
Source:
Bioscience, biotechnology, and biochemistry 2003 v.67 no.4 pp. 783-789
ISSN:
1347-6947
Subject:
Rhodopseudomonas palustris; ammonium chloride; biotechnology; enzyme activity; glutamic acid; molecular weight; pH; porphyrins; pyridoxal phosphate; spectral analysis; substrate specificity; temperature
Abstract:
... Glycine aminotransferase (EC 2.6.1.4; GlyAT) was presumed to be an enzyme concerning the supply of glycine for the extracellular porphyrin production by Rhodopseudomonas palustris No. 7. GlyAT was purified from strain No. 7 as an electrophoretically homogeneous protein. The enzyme was a monomer protein with the molecular weight of about 42,000. From the absorption spectrum of the enzyme (350 nm, 4 ...
DOI:
10.1271/bbb.67.783

28. Catabolism of leucine to branched-chain fatty acids in Staphylococcus xylosus

Author:
Beck, H.C.; Hansen, A.M.; Lauritsen, F.R.
Source:
Journal of applied microbiology 2004 v.96 no.5 pp. 1185-1193
ISSN:
1364-5072
Subject:
Staphylococcus xylosus; aldehydes; alpha-ketoglutaric acid; biosynthesis; branched chain fatty acids; cell membranes; culture media; deamination; flavor; flavor compounds; gas chromatography; glucose; isoleucine; isotope labeling; leucine; mass spectrometry; meat; microbiology; oxidation; pyridoxal phosphate; pyruvic acid; stable isotopes; starter cultures; valine
Abstract:
... Aims: Staphylococcus xylosus is an important starter culture in the production of flavours from the branched-chain amino acids leucine, valine and isoleucine in fermented meat products. The sensorially most important flavour compounds are the branched-chain aldehydes and acids derived from the corresponding amino acids and this paper intends to perspectivate these flavour compounds in the context ...
DOI:
10.1111/j.1365-2672.2004.02253.x

29. Effect of Age on Plasma Homocysteine Concentrations in Young and Elderly Subjects Considering Serum Vitamin Concentrations and Different Lifestyle Factors

Author:
A. Straßburg; C. Krems; P.-M. Lührmann; B. Hartmann; M. Neuhäuser-Berthold
Source:
International journal for vitamin and nutrition research 2004 v.74 no.2 pp. 129-136
ISSN:
0300-9831
Subject:
alcohols; blood serum; elderly; females; folic acid; health status; homocysteine; lifestyle; longitudinal studies; men; methionine; nutrition; pyridoxal phosphate; regression analysis; smoking (habit); tea; vitamin B12; women; young adults
Abstract:
... Objectives: The aim of this study was to investigate whether an increase in total homocysteine (tHcy) concentration with increasing age is due to diminishing serum concentrations of pyridoxal-5-phosphate (PLP), vitamin B-12, and folate. The possible influence of different lifestyle factors on tHcy concentration was considered. Methods: Plasma tHcy, serum concentrations of pyridoxal-5-phosphate, vi ...
DOI:
10.1024/0300-9831.74.2.129

30. Folic acid and vitamin B12 are more effective than vitamin B6 in lowering fasting plasma homocysteine concentration in patients with coronary artery disease

Author:
Lee, B.J.; Huang, M.C.; Chung, L.J.; Cheng, C.H.; Lin, K.L.; Su, K.H.; Huang, Y.C.
Source:
European journal of clinical nutrition 2004 v.58 no.3 pp. 481-487
ISSN:
0954-3007
Subject:
alanine; aspartate transaminase; blood serum; catheters; coronary artery disease; coronary vessels; enzyme activity; erythrocytes; folic acid; homocysteine; hospitals; patients; placebos; pyridoxal phosphate; pyridoxine; veterans; vitamin B12; Taiwan
Abstract:
... Objective: To investigate whether vitamin B6 supplementation had a beneficial effect on lowering fasting plasma homocysteine concentrations in coronary artery disease (CAD) patients. Design: A single-blind intervention study. Setting: The study was performed at the Taichung Veterans General Hospital, the central part of Taiwan. Subjects: A total of 50 subjects were identified by cardiac catheteriz ...
DOI:
10.1038/sj.ejcn.1601834

31. Dietary vitamin B-6 restriction does not alter rates of homocysteine remethylation or synthesis in healthy young women and men

Author:
Davis, S.R.; Scheer, J.B.; Quinlivan, E.P.; Coats, B.S.; Stacpoole, P.W.; Gregory, J.F. III
Source:
American journal of clinical nutrition 2005 v.81 no.3 pp. 648-655
ISSN:
0002-9165
Subject:
human nutrition; men; women; vitamin deficiencies; pyridoxine; homocysteine; methylation; synthesis; methionine; pyridoxal phosphate; serine
Abstract:
... Background: The effects of vitamin B-6 status on steady-state kinetics of homocysteine metabolism in humans are unclear. Objective: The objective was to determine the effects of dietary vitamin B-6 restriction on the rates of homocysteine remethylation and synthesis in healthy humans. Design: Primed, constant infusions of [13C5]methionine, [3-13C]serine, and [2H3]leucine were conducted in healthy ...
PubMed:
15755835

32. Mechanism of α-oxoamine synthases: identification of the intermediate Claisen product in the 8-amino-7-oxononanoate synthase reaction

Author:
Olivier Kerbarh; Dominic J. Campopiano; Robert L. Baxter
Source:
Chemical communications 2005 v. no.1 pp. 60-62
ISSN:
1364-548X
Subject:
alanine; aldimines; chemical reactions; enzymes; pyridoxal phosphate
Abstract:
... The reactive β-ketoacid pyridoxal-5′-phosphate aldimine formed in the condensation step of the 8-amino-7-oxononanoate synthase reaction was ‘trapped’ in the enzyme-bound form by carrying out the reaction with l-alanine methyl ester and pimeloyl-CoA affording the more stable methyl ester of the putative intermediate, the characterisation of which provides the first definitive evidence for a β-ketoa ...
DOI:
10.1039/b511837a

33. Alanine racemase of alfalfa seedlings (Medicago sativa L.): First evidence for the presence of an amino acid racemase in plants

Author:
Ono, K.; Yanagida, K.; Oikawa, T.; Ogawa, T.; Soda, K.
Source:
Phytochemistry 2006 v.67 no.9 pp. 856-860
ISSN:
0031-9422
Subject:
Medicago sativa; alfalfa; seedlings; plant proteins; isomerases; enzyme activity; enzyme inhibition; pyridoxal phosphate; alanine; enzyme substrates; coenzymes
Abstract:
... We demonstrated several kinds of D-amino acids in plant seedlings, and moreover alanine racemase (E.C.5.1.1.1) in alfalfa (Medicago sativa L.) seedlings. This is the first evidence for the presence of amino acid racemase in plant. The enzyme was effectively induced by the addition of L- or D-alanine, and we highly purified the enzyme to show enzymological properties. The enzyme exclusively catalyz ...
DOI:
10.1016/j.phytochem.2006.02.017
PubMed:
16616264

34. Identification and functional analysis of a prokaryotic‐type aspartate aminotransferase: implications for plant amino acid metabolism

Author:
Fernando de la Torre; Laura De Santis; María Fernanda Suárez; Remedios Crespillo; Francisco M. Cánovas
Source:
plant journal 2006 v.46 no.3 pp. 414-425
ISSN:
0960-7412
Subject:
Arabidopsis; Escherichia coli; amino acid metabolism; animals; aspartate transaminase; chloroplasts; genes; nitrogen metabolism; photosynthesis; phylogeny; pyridoxal phosphate; rice
Abstract:
... In this paper, we report the identification of genes from pine (PpAAT), Arabidopsis (AtAAT) and rice (OsAAT) encoding a novel class of aspartate aminotransferase (AAT, EC 2.6.1.1) in plants. The enzyme is unrelated to other eukaryotic AATs from plants and animals but similar to bacterial enzymes. Phylogenetic analysis indicates that this prokaryotic‐type AAT is closely related to cyanobacterial en ...
DOI:
10.1111/j.1365-313X.2006.02713.x
PubMed:
16623902

35. Molecular and biochemical characterization of a serine racemase from Arabidopsis thaliana

Author:
Fujitani, Y.; Ishihara, K.; Oikawa, T.; Ito, K.; Sugimoto, M.
Source:
Phytochemistry 2006 v.67 no.7 pp. 668-674
ISSN:
0031-9422
Subject:
Arabidopsis thaliana; serine; complementary DNA; pyridoxal phosphate; sequence alignment; recombinant fusion proteins; cations; tissue distribution; amino acid sequences; gene expression; isomerases; enzyme kinetics; enzyme substrates; enzyme activity
Abstract:
... A cDNA encoding a homolog of mammalian serine racemase, a unique enzyme in eukaryotes, was isolated from Arabidopsis thaliana and expressed in Escherichia coli cells. The gene product, of which the amino acid residues for binding pyridoxal 5'-phosphate (PLP) are conserved in this as well as mammalian serine racemases, catalyzes not only serine racemization but also dehydration of serine to pyruvat ...
DOI:
10.1016/j.phytochem.2006.01.003
PubMed:
16483618

36. Alanine racemase from the green alga Chlamydomonas reinhardtii

Author:
Nishimura, K.; Tomoda, Y.; Nakamoto, Y.; Kawada, T.; Ishii, Y.; Nagata, Y.
Source:
Amino acids 2007 v.32 no.1 pp. 59-62
ISSN:
0939-4451
Subject:
Chlamydomonas reinhardtii; absorbance; agarose; alanine; ammonium sulfate; fractionation; gel chromatography; microalgae; molecular weight; pH; pyridoxal phosphate
Abstract:
... Chlamydomonas reinhardtii, a unicellular green microalga, could grow to a stationary phase having optical density of 2.0-2.5 at 750 nm in Tris-acetate-phosphate (TAP) medium containing 0.1% D-alanine. D-alanine has no inhibitory effect on growth and induced alanine racemase activity 130-fold more than without D-alanine in the green alga. Although C. reinhardtii cultured in the TAP medium showed al ...
DOI:
10.1007/s00726-006-0352-8

37. Functional Characterization of a Methionine γ-Lyase in Arabidopsis and its Implication in an Alternative to the Reverse Trans-sulfuration Pathway

Author:
Goyer, Aymeric; Collakova, Eva; Shachar-Hill, Yair; Hanson, Andrew D.
Source:
Plant & cell physiology 2007 v.48 no.2 pp. 232-242
ISSN:
0032-0781
Subject:
Arabidopsis thaliana; Escherichia coli; Protozoa; ammonia; cell physiology; cystathionine; cysteine; genes; homocysteine; leaves; methanethiol; methionine; mutants; nitrogen; plantlets; pyridoxal phosphate; recombinant proteins; selenomethionine; starvation; sulfates
Abstract:
... Methionine γ-lyase (MGL) catalyzes the degradation of L-methionine to α-ketobutyrate, methanethiol and ammonia. The Arabidopsis (Arabidopsis thaliana) genome includes a single gene (At1g64660) encoding a protein (AtMGL) with ~35% identity to bacterial and protozoan MGLs. When overexpressed in Escherichia coli, AtMGL allowed growth on L-methionine as sole nitrogen source and conferred a high rate o ...
DOI:
10.1093/pcp/pcl055

38. Plasma pyridoxal-5-phosphate and future risk of myocardial infarction in the European Prospective Investigation into Cancer and Nutrition Potsdam cohort

Author:
Dierkes, Jutta; Weikert, Cornelia; Klipstein-Grobusch, Kerstin; Westphal, Sabine; Luley, Claus; Möhlig, Matthias; Spranger, Joachim; Boeing, Heiner
Source:
American journal of clinical nutrition 2007 v.86 no.1 pp. 214-220
ISSN:
0002-9165
Subject:
adults; myocardial infarction; risk assessment; disease incidence; blood chemistry; nutritional status; pyridoxal phosphate; inflammation; smoking (habit); risk factors; Germany
Abstract:
... BACKGROUND: Retrospective studies indicate that low concentrations of plasma pyridoxal-5-phosphate (PLP) are associated with cardiovascular events; however, few prospective studies of this issue have been conducted. OBJECTIVE: We therefore investigated whether PLP concentrations are independently associated with myocardial infarction (MI) in the European Investigation into Cancer and Nutrition (EP ...
PubMed:
17616783

39. Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334[rightward arrow]Gly mutant enzymes

Author:
Schwarz, Alexandra; Brecker, Lothar; Nidetzky, Bernd
Source:
FEBS journal 2007 v.274 no.19 pp. 5105-5115
ISSN:
1742-464X
Subject:
Corynebacterium callunae; active sites; arsenates; binding capacity; carbohydrate binding; carbon; catalytic activity; enzyme substrates; glucose; hydrogen bonding; ligands; mutants; nuclear magnetic resonance spectroscopy; pH; phosphates; phosphorylase; pyridoxal phosphate; spectral analysis; starch
Abstract:
... His334 facilitates catalysis by Corynebacterium callunae starch phosphorylase through selective stabilization of the transition state of the reaction, partly derived from a hydrogen bond between its side chain and the C-6 hydroxy group of the glucosyl residue undergoing transfer to and from phosphate. We have substituted His334 by a Gly and measured the disruptive effects of the site-directed repl ...
DOI:
10.1111/j.1742-4658.2007.06030.x

40. Properties of an unusual heme cofactor in PLP-dependent cystathionine β-synthase

Author:
Sangita Singh; Peter Madzelan; Ruma Banerjee
Source:
Natural product reports 2007 v.24 no.3 pp. 631-639
ISSN:
1460-4752
Subject:
active sites; crystal structure; cystathionine; heme; heme iron; heme proteins; homocysteine; ligands; oxidation; pyridoxal phosphate; serine; signal transduction; spectroscopy
Abstract:
... Cystathionine β-synthase is a pyridoxal phosphate (PLP)-dependent hemeprotein, which catalyzes the condensation of serine and homocysteine to give cystathionine, the first step in the transsulfuration pathway. The association of heme with this enzyme in some organisms, including humans, is novel since this combination of cofactors has not been seen in any other protein to date. While the role of P ...
DOI:
10.1039/b604182p

41. The S subunit of d-ornithine aminomutase from Clostridium sticklandii is responsible for the allosteric regulation in d-α-lysine aminomutase

Author:
Tseng, Cheng-Hsing; Yang, Cheng-His; Lin, Heng-Ju; Wu, Chunhung; Chen, Hao-Ping
Source:
FEMS microbiology letters 2007 v.274 no.1 pp. 148-153
ISSN:
0378-1097
Subject:
Clostridium sticklandii; adenosine triphosphate; ammonium compounds; enzymes; lysine; ornithine; pyridoxal phosphate
Abstract:
... Ornithine and lysine are degraded in quite a similar way in Clostridium sticklandii. Both pathways involve adenosylcobalamin-dependent enzymes, d-ornithine 4,5-aminomutase and lysine 5,6-aminomutase. According to previous reports, lysine 5,6-aminomutase is an ATP-dependent allosteric enzyme with many different activators and inhibitors. However, recent studies indicate that ATP does not have a reg ...
DOI:
10.1111/j.1574-6968.2007.00820.x

42. B-vitamins, homocysteine and gene polymorphism in adults with fasting or post-methionine loading hyperhomocysteinemia

Author:
Cheng, Chien-Hsiung; Huang, Yi-Chia; Chen, Feng-Pan; Chou, Ming-Chih; Tsai, Tsung-Po
Source:
European journal of nutrition 2008 v.47 no.8 pp. 491-498
ISSN:
1436-6207
Subject:
adults; cross-sectional studies; fasting; folic acid; genetic polymorphism; genotype; homocysteine; methionine; methylenetetrahydrofolate reductase; pyridoxal phosphate
Abstract:
... Background Although fasting and post-methionine loading (PML) homocysteine concentrations are not necessarily related, a high percentage of hyperhomocysteinemia cases would be missed if methionine loading was not performed. Aim of the study The influences of B-vitamins and genetic polymorphism (methylenetetrahydrofolate reductase 677C [rightward arrow] T, MTHFR 677C [rightward arrow] T) on fasting ...
DOI:
10.1007/s00394-008-0752-5
PubMed:
18979127

43. Cloning of the Gene Encoding α-Methylserine Hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and Characterization of the Recombinant Enzyme

Author:
Hiroyuki NOZAKI; Shinji KURODA; Kunihiko WATANABE; Kenzo YOKOZEKI
Source:
Bioscience, biotechnology, and biochemistry 2008 v.72 no.11 pp. 3002-3005
ISSN:
1347-6947
Subject:
Aminobacter; Ensifer; Escherichia coli; genes; pyridoxal; pyridoxal phosphate; tetrahydrofolic acid; transferases
Abstract:
... Genes encoding α-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 were cloned and expressed in Escherichia coli. The purified enzymes were homodimers with a 46-kDa subunit and contained 1 mol/mol-subunit of pyridoxal 5′-phosphate. The V ₘₐₓ of these enzymes catalyzing the conversion of α-methyl-L-serine to D-alanine via tetrahydrofolate was 22.1 U/mg (AJ ...
DOI:
10.1271/bbb.80255

44. Filling the gap of intracellular dephosphorylation in the Plasmodium falciparum vitamin B1 biosynthesis

Author:
Knöckel, Julia; Bergmann, Bärbel; Müller, Ingrid B.; Rathaur, Sushma; Walter, Rolf D.; Wrenger, Carsten
Source:
Molecular and biochemical parasitology 2008 v.157 no.2 pp. 241-243
ISSN:
0166-6851
Subject:
Northern blotting; Plasmodium falciparum; bacteria; biosynthesis; cytosol; dephosphorylation; enzymes; fungi; humans; malaria; nucleotides; parasites; pyridoxal phosphate; substrate specificity; sugars; thiamin; transfection
Abstract:
... Thiamine pyrophosphate (TPP), the active form of vitamin B1, is an essential cofactor for several enzymes. Humans depend exclusively on the uptake of vitamin B1, whereas bacteria, plants, fungi and the malaria parasite Plasmodium falciparum are able to synthesise thiamine monophosphate (TMP) de novo. TMP has to be dephosphorylated prior to pyrophosphorylation in order to obtain TPP. In P. falcipar ...
DOI:
10.1016/j.molbiopara.2007.10.010

45. Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and l-allo-Thr cleavage

Author:
Bhavani, B.S.; Rajaram, V.; Bisht, Shveta; Kaul, Purnima; Prakash, V.; Murthy, M.R.N.; Appaji Rao, N.; Savithri, H.S.
Source:
FEBS journal 2008 v.275 no.18 pp. 4606-4619
ISSN:
1742-464X
Subject:
Geobacillus stearothermophilus; X-radiation; glycine hydroxymethyltransferase; mutants; mutation; pyridoxal; pyridoxal phosphate; tetrahydrofolic acid; transamination; tyrosine
Abstract:
... Serine hydroxymethyltransferase (SHMT) from Bacillus stearothermophilus (bsSHMT) is a pyridoxal 5'-phosphate-dependent enzyme that catalyses the conversion of l-serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. In addition, the enzyme catalyses the tetrahydrofolate-independent cleavage of 3-hydroxy amino acids and transamination. In this article, we have examined the mech ...
DOI:
10.1111/j.1742-4658.2008.06603.x

46. Low Plasma Pyridoxal 5’-phosphate Concentration and MTHFR 677C→T Genotypes are Associated with Increased Risk of Hypertension

Author:
P.-T. Lin; C.-H. Cheng; J. C.-C. Wei; Y.-C. Huang
Source:
International journal for vitamin and nutrition research 2008 v.78 no.1 pp. 33-40
ISSN:
0300-9831
Subject:
vitamin B12; nutritional status; pyridoxal phosphate; blood chemistry; genetic polymorphism; alleles; hypertension; risk assessment; methylenetetrahydrofolate reductase; risk factors; genotype; folic acid; genetic variation; blood pressure; adults; homocysteine
Abstract:
... Few studies have linked homocysteine, B vitamins and/or genetic defects to the risk of hypertension. The purpose of this study was to investigate homocysteine, B-vitamins, and genetic mutation in relation to the risk of hypertension. Subjects were assigned to the hypertension (HTN) group (n = 50) or non-hypertension (non-HTN) group (n = 123). All subjects’ blood pressure (systolic blood pressure, ...
DOI:
10.1024/0300-9831.78.1.33

47. Plasma pyridoxal 5'-phosphate and high-sensitivity C-reactive protein are independently associated with an increased risk of coronary artery disease

Author:
Cheng, Chien-Hsiung; Lin, Ping-Ting; Liaw, Yung-Po; Ho, Chien-Chang; Tsai, Tsung-Po; Chou, Ming-Chih; Huang, Yi-Chia
Source:
Nutrition 2008 v.24 no.3 pp. 239-244
ISSN:
0899-9007
Subject:
C-reactive protein; nutritional status; pyridoxal phosphate; atherosclerosis; inflammation; risk factors; pyridoxine; coronary vessels; patients; synergism; case studies; homocysteine
Abstract:
... Objective: Whether vitamin B6 exerts an independent or a synergic effect in combination with inflammation for the risk of coronary artery disease (CAD) is unclear. The purpose of this study was to investigate whether plasma pyridoxal 5'-phosphate (PLP) is dependent on or independent of the inflammation marker C-reactive protein (CRP) to associate with the risk of CAD. Methods: This was a hospital- ...
DOI:
10.1016/j.nut.2007.12.003
PubMed:
18312786

48. Preventing cell death induced by carbonyl stress, oxidative stress or mitochondrial toxins with vitamin B anti-AGE agents

Author:
Mehta, Rhea; Shangari, Nandita; O'Brien, Peter J.
Source:
Molecular nutrition & food research 2008 v.52 no.3 pp. 379-385
ISSN:
1613-4125
Subject:
glycation; cell death; lipid peroxidation; lipids; autoxidation; thiamin; oxidative stress; protective effect; carbohydrates; pyridoxine; toxins; animal disease models; chelating agents; copper; cytotoxicity; vitamin supplements; diabetes; acrolein; antioxidants; cyanides; pyridoxal phosphate; metabolic detoxification; beers
Abstract:
... Carbonyls generated by autoxidation of carbohydrates or lipid peroxidation have been implicated in advanced glycation end product (AGE) formation in tissues adversely affected by diabetes complications. Tissue AGE and associated pathology have been decreased by vitamin B₁/B₆ in trials involving diabetic animal models. To understand the molecular cytoprotective mechanisms involved, the effects of B ...
DOI:
10.1002/mnfr.200600190
PubMed:
17918169

49. Production of γ-aminobutyric acid by Streptococcus salivarius subsp. thermophilus Y2 under submerged fermentation

Author:
Yang, S.-Y.; Lü, F.-X.; Lu, Z.-X.; Bie, X.-M.; Jiao, Y.; Sun, L.-J.; Yu, B.
Source:
Amino acids 2008 v.34 no.3 pp. 473-478
ISSN:
0939-4451
Subject:
Streptococcus thermophilus; biotransformation; central nervous system; cheeses; drugs; functional foods; gamma-aminobutyric acid; glutamate decarboxylase; pH; pyridoxal phosphate; submerged fermentation; temperature; yogurt
Abstract:
... γ-Aminobutyric acid (GABA), a major inhibitory neurotransmitter in the central nervous system, has several well-known physiological functions and has been applied to the production of many drugs and functional foods. The technology of GABA production via submerged fermentation by Streptococcus salivarius subsp. thermophilus Y2 was investigated in this paper. It indicated that the GABA production w ...
DOI:
10.1007/s00726-007-0544-x

50. Public Health Significance of Elevated Homocysteine

Author:
Jacob Selhub
Source:
Food and nutrition bulletin v.29 no.2_suppl1 pp. S116
ISSN:
0379-5721
Subject:
Alzheimer disease; bone fractures; coenzymes; elderly; folic acid; heart failure; homocysteine; men; metabolism; mortality; neural tube defects; pre-eclampsia; pregnant women; public health; pyridoxal phosphate; pyridoxine; risk factors; stroke; vitamin B12; vitamin status
Abstract:
... Homocysteine is a sulfur amino acid whose metabolism stands at the intersection of two pathways: remethylation, which requires folic acid and vitamin B₁₂ coenzymes; and transsulfuration, which requires pyridoxal-5′-phosphate, the vitamin B₆ coenzyme. Data from a number of laboratories suggest that mild elevations of homocysteine in plasma are a risk factor for occlusive vascular disease. In the Fr ...
DOI:
10.1177/15648265080292S116