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You searched for: Subject pyridoxal phosphate Remove constraint Subject: pyridoxal phosphate Subject term pyridoxal phosphate Remove constraint Subject term: pyridoxal phosphate
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1. Degradation and binding to food proteins of vitamin B-6 compounds during thermal processing

Author:
Gregory, J.F. III.; Ink, S.L.; Sartain, D.B.
Source:
Journal of food science 1986 v.51 no.5 pp. 1345-1351
ISSN:
0022-1147
Subject:
food processing; heat treatment; pyridoxine; pyridoxal; pyridoxal phosphate; pyridoxamine; degradation; proteins
DOI:
10.1111/j.1365-2621.1986.tb13119.x

2. Mechanistic deductions from kinetic isotope effects and pH studies of pyridoxal phosphate dependent carbon-carbon lyases: Erwinia herbicola and Citrobacter freundii tyrosine phenol-lyase

Author:
Kiick, D.M.; Phillips, R.S.
Source:
Biochemistry 1988 v.27 no.19 pp. 7333-7338
ISSN:
0006-2960
Subject:
Pantoea agglomerans; Citrobacter freundii; pH; pyridoxal phosphate; carbon; lyases; kinetics; tyrosine
DOI:
10.1021/bi00419a023
PubMed:
3207679

3. Inactivation of chloroplast H+-ATPase by modification of Lysbeta 359, Lysalpha 176 and Lysalpha 266

Author:
Horbach, M.; Meyer, H.E.; Bickel-Sandkotter, S.
Source:
European journal of biochemistry 1991 v.200 no.2 pp. 449-456
ISSN:
0014-2956
Subject:
chloroplasts; adenosinetriphosphatase; pyridoxal phosphate; magnesium; lysine; enzyme activity; inactivation; molecular conformation; protein structure; binding sites
Abstract:
... Treatment of isolated, latent chloroplast ATPase with pyridoxal-5-phosphate (pyridoxal-P) in presence of Mg2+ causes inhibition of dithiothreitol-activated plus heat-activated ATP hydrolysis. The amount of [3H]pyridoxal-P bound to chloroplast coupling factor 1 (CF1) was estimated to run up to 6 +/- 1 pyridoxal-P/enzyme, almost equally distributed between the alpha- and beta-subunits. Inactivation, ...
DOI:
10.1111/j.1432-1033.1991.tb16203.x
PubMed:
1832378

4. Transport processes and corresponding changes in metabolite levels in relation to starch synthesis in barley (Hordeum vulgare L.) etioplasts

Author:
Batz, O.; Scheibe, R.; Neuhaus, H.E.; Transport processes and corresponding changes in metabolite levels in relation to starch synthesis in barley (Hordeum vulgare L.) etioplasts.
Source:
Plant physiology 1992 v.100 no.1 pp. 184-190
ISSN:
0032-0889
Subject:
Hordeum vulgare; starch; carbohydrate metabolism; etioplasts; adenosine triphosphate; pyridoxal phosphate; glucose 6-phosphate; active transport; enzymes; enzyme activity; inorganic phosphorus
Abstract:
... Intact etioplasts with an intactness of 85% and with a cytosolic and a mitochondrial contamination of less than 10% were isolated from 8-d-old dark-grown barley (Hordeum vulgare) leaves. These plastids contained starch equivalent to 21.5 micromoles of glucose per mg protein. From various likely precursors applied to isolated etioplasts, only dihydroxyacetone phosphate (DHAP) had significant effect ...
DOI:
10.1104/pp.100.1.184
PubMed:
16652944

5. Vitamin status and intake as primary determinants of homocysteinemia in an elderly population

Author:
Selhub, J.; Jacques, P.F.; Wilson, P.W.; Rush, D.; Rosenberg, I.H.
Source:
The Journal of the American Medical Association : JAMA 1993 v.270 no.22 pp. 2693-2698
ISSN:
0098-7484
Subject:
nutrient intake; elderly; vitamin B12; blood serum; pyridoxal phosphate; human health; folic acid; cross-sectional studies; homocysteine; Massachusetts
Abstract:
... To describe the distribution of plasma homocysteine concentrations in an elderly population and to analyze the relationship between homocysteine level and intake of vitamins and serum levels of vitamins that serve as coenzymes in homocysteine metabolism. DESIGN--Cross-sectional analysis of homocysteine levels and vitamin blood levels and intake in elderly participants in the Framingham Study. SETT ...
Handle:
10113/103
DOI:
10.1001/jama.1993.03510220049033

6. Mice lacking tissue non-specific alkaline phosphatase die from seizures due to defective metabolism of vitamin B-6

Author:
Waymire, K.G.; Mahuren, J.D.; Jaje, J.M.; Guilarte, T.R.; Coburn, S.P.; MacGregor, G.R.
Source:
Nature genetics 1995 v.11 no.1 pp. 45-51
ISSN:
1061-4036
Subject:
mice; pyridoxal phosphate; pyridoxine; metabolism; metabolic diseases; alkaline phosphatase; enzyme deficiencies; teeth; structural genes; homologous recombination; nervous system diseases; gamma-aminobutyric acid; brain
DOI:
10.1038/ng0995-45
PubMed:
7550313

7. Energizing effects of illumination on the reactivities of lysine residues of the gamma-subunit of chloroplast ATP synthase

Author:
Komatsu-Takaki, M.
Source:
European journal of biochemistry 1996 v.236 no.2 pp. 470-475
ISSN:
0014-2956
Subject:
H+/K+-exchanging ATPase; H-transporting ATP synthase; ammonium chloride; electrochemistry; lighting; lysine; protons; pyridoxal phosphate; thylakoids
Abstract:
... Incubation of chloroplast thylakoids with pyridoxal 5'-phosphate for a short time (5 s) modified the lysine residues of the gamma subunit of ATP synthase. Energization of thylakoids by illumination increased the reactivity of Lys24 by a factor of three and decreased the reactivity of Lys30 to 60%. The reactivities of these residues reached their maximum and minimum values, respectively, within 1 s ...
DOI:
10.1111/j.1432-1033.1996.00470.x
PubMed:
8612618

8. Plasma homocysteine as a risk factor for atherothrombotic events in systemic lupus erythematosus

Author:
Petri, M.; Dallal, G.E.; Nadeau, M.R.; Selhub, J.; Rosenberg, I.H.
Source:
The Lancet 1996 v.348 no.9035 pp. 1120-1124
ISSN:
0099-5355
Subject:
lupus erythematosus; thrombosis; prospective studies; vitamin B12; pyridoxal phosphate; risk factors; human health; coronary vessels; folic acid; stroke; blood plasma; homocysteine
Abstract:
... Background: The aim of this study was to asses whether plasma homocysteine is a risk factor for stroke and other thrombotic events in patients with systemic lupus erythematosus (SLE)-a condition known to be associated with premature atherothrombotic complications. Methods: In this prospective study, we investigated the association between homocysteine and risk of stroke and thrombotic events in 33 ...
Handle:
10113/119
DOI:
10.1016/S0140-6736(96)03032-2

9. Spinach chloroplast O-acetylserine (thiol)-lyase exhibits two catalytically non-equivalent pyridoxal-5'-phosphate-containing active sites

Author:
Rolland, N.; Ruffet, M.L.; Job, D.; Douce, R.; Droux, M.
Source:
European journal of biochemistry 1996 v.236 no.1 pp. 272-282
ISSN:
0014-2956
Subject:
DNA-directed RNA polymerase; Escherichia coli; absorbance; acrylamides; active sites; affinity chromatography; agarose; cell culture; chloroplasts; fluorescence; iodides; nitrobenzoic acids; physical properties; pyridoxal phosphate; quantitative analysis; recombinant proteins; schiff bases; spinach; synthetic genes; thiols; tryptophan
Abstract:
... A synthetic gene encoding the mature spinach-chloroplast O-acetylserine (thiol)-lyase was constructed and expressed in an Escherichia coli strain carrying the T7 RNA polymerase system. The pure recombinant protein was obtained at high yield (6 mg/l cell culture) using a new purification procedure that includes affinity chromatography on Green A agarose. Its specific activity was of the order of 10 ...
DOI:
10.1111/j.1432-1033.1996.00272.x

10. Partial purification and characterisation of an aromatic amino acid aminotransferase from mung bean (Vigna radiata L. Wilczek)

Author:
Simpson, R.M.; Nonhebel, H.M.; Christie, D.L.
Source:
Planta 1997 v.201 no.1 pp. 71-77
ISSN:
0032-0935
Subject:
pyridoxal phosphate; molecular weight; transaminases; aspartic acid; Vigna radiata; tryptophan; keto acids; indole acetic acid; naphthaleneacetic acid; chemical constituents of plants; enzyme activity; amino acid metabolism; leaves; shoots
Abstract:
... An aromatic amino acid aminotransferase (aromAT) was purified over 33 000-fold from the shoots and primary leaves of mung beans (Vigna radiata L. Wilczek). The enzyme was purified by ammonium sulfate precipitation, gel filtration and anion exchange followed by fast protein liquid chromatography using Mono Q and Phenylsuperose. The relative aminotransferase activities using the most active amino ac ...
DOI:
10.1007/BF01258682

11. Differential hydrolysis of alk(en)yl cysteine sulphoxides by alliinase in onion macerates: flavour implications

Author:
Lancaster, J.E.; Shaw, M.L.; Randle, W.M.
Source:
Journal of the science of food and agriculture 1998 v.78 no.3 pp. 367-372
ISSN:
0022-5142
Subject:
onions; mechanical damage; organic sulfur compounds; hydrolysis; enzyme activity; lyases; pyruvic acid; peptides; cultivars; Allium cepa; sulfur; nutrient availability; pyridoxal phosphate
Abstract:
... Intact cells of Allium spp have no odour, but when cells are disrupted, the enzyme alliinase (EC 4.4.1.4) hydrolyses the S-alk(en)yl sulphoxides (ACSOs) to produce pyruvate, ammonia and the many volatile sulphur compounds associated with flavour and odour. In onions, there are three main sulphoxides: methyl, propyl and 1-propenyl, which gives rise to onion's tear-producing effect. The relative pro ...
DOI:
10.1002/(SICI)1097-0010(199811)78:3<367::AID-JSFA127>3.0.CO;2-N

12. Relationship between cigarette smoking and nutrient intakes and blood status indices of older people living in the UK: further analysis of data from the National Diet and Nutrition Survey of people aged 65 years and over, 1994/95

Author:
Walmsley, C.M.; Bates, C.J.; Pentice, A.; Cole, T.J.
Source:
Public health nutrition 1999 v.2 no.2 pp. 199-208
ISSN:
1368-9800
Subject:
nutrient intake; tocopherols; elderly; food intake; nutritional status; blood serum; men; women; pyridoxal phosphate; smoking (habit); erythrocytes; carotenoids; oxidation; folic acid; nutrients; dietary surveys; antioxidants; ascorbic acid; United Kingdom
PubMed:
10447248

13. Pyridoxal 5′‐phoshate Schiff base in Citrobacter freundii tyrosinephenol‐lyase: Ionic and tautomeric equilibria

Author:
Natalia P. Bazhulina; Yurii V. Morozov; Anastasia I. Papisova; Tatyana V. Demidkina
Source:
European journal of biochemistry 2000 v.267 no.6 pp. 1830-1836
ISSN:
0014-2956
Subject:
Citrobacter freundii; absorption; lognormal distribution; nitrogen; pH; potassium; pyridoxal; pyridoxal phosphate; schiff bases; sodium; spectral analysis; tyrosine
Abstract:
... Spectral properties of the internal Schiff base in tyrosine phenol‐lyase have been investigated in the presence of an activating cation K+ and a cation‐inhibitor Na+. The holoenzyme absorption spectra in the pH range 6.5–8.7 were recorded in the presence of K+. No apparent pKa value of the coenzyme chromophore was found in this pH range, indicating that the internal Schiff base does not chan ...
DOI:
10.1046/j.1432-1327.2000.01185.x

14. Effect of phosphate on stability of pyridoxal in the presence of lysine

Author:
Huang, T.C.; Chen, M.H.; Ho, C.T.
Source:
Journal of agricultural and food chemistry 2001 v.49 no.3 pp. 1559-1563
ISSN:
0021-8561
Subject:
lysine; pyridoxal phosphate; chemical reactions; amino acid derivatives
Abstract:
... The stability of the biologically active compound vitamin B6 in aqueous solution was investigated. Schiff base formation is the major reaction between the epsilon-amino group of lysine and the aldehyde group of both pyridoxal and pyridoxal phosphate. Model systems composed of equal molar concentrations of lysine with either pyridoxal or pyridoxal phosphate were used to study the effect of proton t ...
DOI:
10.1021/jf000886q
PubMed:
11312896

15. The biosynthesis of ovothiol A (N1‐methyl‐4‐mercaptohistidine) : Identification of S‐(4′‐l‐histidyl)‐l‐cysteine sulfoxide as an intermediate and the products of the sulfoxide lyase reaction

Author:
Ryan N. Vogt; Hendrik S. C. Spies; Daniel J. Steenkamp
Source:
European journal of biochemistry 2001 v.268 no.20 pp. 5229-5241
ISSN:
0014-2956
Subject:
Crithidia fasciculata; NAD (coenzyme); NADH dehydrogenase; ammonium sulfate; anion exchange chromatography; biosynthesis; cysteine; dithiothreitol; high performance liquid chromatography; histidine; iron; isotope labeling; lactate dehydrogenase; oxygen; pyridoxal phosphate; pyruvic acid; spectral analysis; spectroscopy; sulfur; thiols
Abstract:
... Crude extracts of Crithidia fasciculata catalyse the formation of 4‐mercapto‐l‐histidine, an intermediate in the biosynthesis of ovothiol A (N1‐methyl‐4‐mercaptohistidine), in the presence of histidine, cysteine, Fe2+ and pyridoxal phosphate. This activity was present in a 35–55% ammonium sulfate fraction that was shown to produce a transsulfuration intermediate in the absence of pyr ...
DOI:
10.1046/j.0014-2956.2001.02444.x

16. Oral contraceptives and nutritional status in adolescent British girls

Author:
Thane, Christopher W.; Bates, Christopher J.; Prentice, Ann
Source:
Nutrition research 2002 v.22 no.4 pp. 449-462
ISSN:
0271-5317
Subject:
adolescents; alpha-tocopherol; ascorbic acid; beta-carotene; blood serum; body mass index; dietary surveys; elderly; gamma-tocopherol; girls; high density lipoprotein cholesterol; low density lipoprotein cholesterol; nutritional status; oral contraceptives; pyridoxal phosphate; triacylglycerols; vitamin A; vitamin B12; zinc
Abstract:
... Associations between oral contraceptive pill (OC) use and vitamin, mineral and lipid status were examined in 117 postmenarcheal 16-to-18-year-old British girls, from the 1997 National Diet and Nutrition Survey of young people aged 4 to 18 years. Thirty-nine were taking OCs at the time of blood, anthropometric and sociodemographic assessment, while seventy-eight were not. After adjusting for age, b ...

17. Maternal periconceptional biochemical and hematological parameters, vitamin profiles and pregnancy outcome

Author:
Weerd, S. de; Steegers-Theunissen, R.P.M.; Boo, T.M. de; Thomas, C.M.G.; Steegers, E.A.P.
Source:
European journal of clinical nutrition 2003 v.57 no.9 pp. 1128-1134
ISSN:
0954-3007
Subject:
amenorrhea; birth weight; blood sampling; blood serum; embryogenesis; ferritin; folic acid; hematocrit; hemoglobin; hospitals; iron; longitudinal studies; pregnancy outcome; protein content; pyridoxal phosphate; riboflavin; risk; thiamin; uric acid; vitamin A; vitamin B12; women; Netherlands
Abstract:
... Objectives: To evaluate periconceptional maternal biochemical and hematological parameters and vitamin profiles in relation to the risk of early pregnancy loss and birth weight. Design: Prospective longitudinal study. Setting: University Medical Centre Nijmegen, Academic Medical Centre, Amsterdam, Maria and Elisabeth Hospitals, Tilburg, and Catharina Hospital, Eindhoven, The Netherlands. Subjects: ...
DOI:
10.1038/sj.ejcn.1601654

18. Catabolism of leucine to branched-chain fatty acids in Staphylococcus xylosus

Author:
Beck, H.C.; Hansen, A.M.; Lauritsen, F.R.
Source:
Journal of applied microbiology 2004 v.96 no.5 pp. 1185-1193
ISSN:
1364-5072
Subject:
Staphylococcus xylosus; aldehydes; alpha-ketoglutaric acid; biosynthesis; branched chain fatty acids; cell membranes; culture media; deamination; flavor; flavor compounds; gas chromatography; glucose; isoleucine; isotope labeling; leucine; mass spectrometry; meat; microbiology; oxidation; pyridoxal phosphate; pyruvic acid; stable isotopes; starter cultures; valine
Abstract:
... Aims: Staphylococcus xylosus is an important starter culture in the production of flavours from the branched-chain amino acids leucine, valine and isoleucine in fermented meat products. The sensorially most important flavour compounds are the branched-chain aldehydes and acids derived from the corresponding amino acids and this paper intends to perspectivate these flavour compounds in the context ...
DOI:
10.1111/j.1365-2672.2004.02253.x

19. Effect of Age on Plasma Homocysteine Concentrations in Young and Elderly Subjects Considering Serum Vitamin Concentrations and Different Lifestyle Factors

Author:
A. Straßburg; C. Krems; P.-M. Lührmann; B. Hartmann; M. Neuhäuser-Berthold
Source:
International journal for vitamin and nutrition research 2004 v.74 no.2 pp. 129-136
ISSN:
0300-9831
Subject:
alcohols; blood serum; elderly; females; folic acid; health status; homocysteine; lifestyle; longitudinal studies; men; methionine; nutrition; pyridoxal phosphate; regression analysis; smoking (habit); tea; vitamin B12; women; young adults
Abstract:
... Objectives: The aim of this study was to investigate whether an increase in total homocysteine (tHcy) concentration with increasing age is due to diminishing serum concentrations of pyridoxal-5-phosphate (PLP), vitamin B-12, and folate. The possible influence of different lifestyle factors on tHcy concentration was considered. Methods: Plasma tHcy, serum concentrations of pyridoxal-5-phosphate, vi ...
DOI:
10.1024/0300-9831.74.2.129

20. Folic acid and vitamin B12 are more effective than vitamin B6 in lowering fasting plasma homocysteine concentration in patients with coronary artery disease

Author:
Lee, B.J.; Huang, M.C.; Chung, L.J.; Cheng, C.H.; Lin, K.L.; Su, K.H.; Huang, Y.C.
Source:
European journal of clinical nutrition 2004 v.58 no.3 pp. 481-487
ISSN:
0954-3007
Subject:
alanine; aspartate transaminase; blood serum; catheters; coronary artery disease; coronary vessels; enzyme activity; erythrocytes; folic acid; homocysteine; hospitals; patients; placebos; pyridoxal phosphate; pyridoxine; veterans; vitamin B12; Taiwan
Abstract:
... Objective: To investigate whether vitamin B6 supplementation had a beneficial effect on lowering fasting plasma homocysteine concentrations in coronary artery disease (CAD) patients. Design: A single-blind intervention study. Setting: The study was performed at the Taichung Veterans General Hospital, the central part of Taiwan. Subjects: A total of 50 subjects were identified by cardiac catheteriz ...
DOI:
10.1038/sj.ejcn.1601834

21. Identification and functional analysis of a prokaryotic‐type aspartate aminotransferase: implications for plant amino acid metabolism

Author:
Fernando de la Torre; Laura De Santis; María Fernanda Suárez; Remedios Crespillo; Francisco M. Cánovas
Source:
plant journal 2006 v.46 no.3 pp. 414-425
ISSN:
0960-7412
Subject:
Arabidopsis; Escherichia coli; amino acid metabolism; animals; aspartate transaminase; chloroplasts; genes; nitrogen metabolism; photosynthesis; phylogeny; pyridoxal phosphate; rice
Abstract:
... In this paper, we report the identification of genes from pine (PpAAT), Arabidopsis (AtAAT) and rice (OsAAT) encoding a novel class of aspartate aminotransferase (AAT, EC 2.6.1.1) in plants. The enzyme is unrelated to other eukaryotic AATs from plants and animals but similar to bacterial enzymes. Phylogenetic analysis indicates that this prokaryotic‐type AAT is closely related to cyanobacterial en ...
DOI:
10.1111/j.1365-313X.2006.02713.x
PubMed:
16623902

22. Structure of a bacterial pyridoxal 5'-phosphate synthase complex

Author:
Strohmeier, Marco; Raschle, Thomas; Mazurkiewicz, Jacek; Rippe, Karsten; Sinning, Irmgard; Fitzpatrick, Teresa B.; Tews, Ivo
Source:
Proceedings of the National Academy of Sciences of the United States of America 2006 v.103 no.51 pp. 19284-19289
ISSN:
0027-8424
Subject:
active sites; ammonia; biosynthesis; catalytic activity; fungi; glutaminase; glutamine; humans; hydrophobicity; mutagenesis; pentoses; prokaryotic cells; pyridoxal phosphate
Abstract:
... Vitamin B₆ is an essential metabolic cofactor that has more functions in humans than any other single nutrient. Its de novo biosynthesis occurs through two mutually exclusive pathways that are absent in animals. The predominant pathway found in most prokaryotes, fungi, and plants has only recently been discovered. It is distinguished by a glutamine amidotransferase, which is remarkable in that it ...
DOI:
10.1073/pnas.0604950103
PubMed:
17159152
PubMed Central:
PMC1748218

23. Alanine racemase from the green alga Chlamydomonas reinhardtii

Author:
Nishimura, K.; Tomoda, Y.; Nakamoto, Y.; Kawada, T.; Ishii, Y.; Nagata, Y.
Source:
Amino acids 2007 v.32 no.1 pp. 59-62
ISSN:
0939-4451
Subject:
Chlamydomonas reinhardtii; absorbance; agarose; alanine; ammonium sulfate; fractionation; gel chromatography; microalgae; molecular weight; pH; pyridoxal phosphate
Abstract:
... Chlamydomonas reinhardtii, a unicellular green microalga, could grow to a stationary phase having optical density of 2.0-2.5 at 750 nm in Tris-acetate-phosphate (TAP) medium containing 0.1% D-alanine. D-alanine has no inhibitory effect on growth and induced alanine racemase activity 130-fold more than without D-alanine in the green alga. Although C. reinhardtii cultured in the TAP medium showed al ...
DOI:
10.1007/s00726-006-0352-8

24. Functional Characterization of a Methionine γ-Lyase in Arabidopsis and its Implication in an Alternative to the Reverse Trans-sulfuration Pathway

Author:
Goyer, Aymeric; Collakova, Eva; Shachar-Hill, Yair; Hanson, Andrew D.
Source:
Plant & cell physiology 2007 v.48 no.2 pp. 232-242
ISSN:
0032-0781
Subject:
Arabidopsis thaliana; Escherichia coli; Protozoa; ammonia; cell physiology; cystathionine; cysteine; genes; homocysteine; leaves; methanethiol; methionine; mutants; nitrogen; plantlets; pyridoxal phosphate; recombinant proteins; selenomethionine; starvation; sulfates
Abstract:
... Methionine γ-lyase (MGL) catalyzes the degradation of L-methionine to α-ketobutyrate, methanethiol and ammonia. The Arabidopsis (Arabidopsis thaliana) genome includes a single gene (At1g64660) encoding a protein (AtMGL) with ~35% identity to bacterial and protozoan MGLs. When overexpressed in Escherichia coli, AtMGL allowed growth on L-methionine as sole nitrogen source and conferred a high rate o ...
DOI:
10.1093/pcp/pcl055

25. Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334[rightward arrow]Gly mutant enzymes

Author:
Schwarz, Alexandra; Brecker, Lothar; Nidetzky, Bernd
Source:
FEBS journal 2007 v.274 no.19 pp. 5105-5115
ISSN:
1742-464X
Subject:
Corynebacterium callunae; active sites; arsenates; binding capacity; carbohydrate binding; carbon; catalytic activity; enzyme substrates; glucose; hydrogen bonding; ligands; mutants; nuclear magnetic resonance spectroscopy; pH; phosphates; phosphorylase; pyridoxal phosphate; spectral analysis; starch
Abstract:
... His334 facilitates catalysis by Corynebacterium callunae starch phosphorylase through selective stabilization of the transition state of the reaction, partly derived from a hydrogen bond between its side chain and the C-6 hydroxy group of the glucosyl residue undergoing transfer to and from phosphate. We have substituted His334 by a Gly and measured the disruptive effects of the site-directed repl ...
DOI:
10.1111/j.1742-4658.2007.06030.x

26. The S subunit of d-ornithine aminomutase from Clostridium sticklandii is responsible for the allosteric regulation in d-α-lysine aminomutase

Author:
Tseng, Cheng-Hsing; Yang, Cheng-His; Lin, Heng-Ju; Wu, Chunhung; Chen, Hao-Ping
Source:
FEMS microbiology letters 2007 v.274 no.1 pp. 148-153
ISSN:
0378-1097
Subject:
Clostridium sticklandii; adenosine triphosphate; ammonium compounds; enzymes; lysine; ornithine; pyridoxal phosphate
Abstract:
... Ornithine and lysine are degraded in quite a similar way in Clostridium sticklandii. Both pathways involve adenosylcobalamin-dependent enzymes, d-ornithine 4,5-aminomutase and lysine 5,6-aminomutase. According to previous reports, lysine 5,6-aminomutase is an ATP-dependent allosteric enzyme with many different activators and inhibitors. However, recent studies indicate that ATP does not have a reg ...
DOI:
10.1111/j.1574-6968.2007.00820.x

27. β-Nicotinamide adenine dinucleotide is an inhibitory neurotransmitter in visceral smooth muscle

Author:
Mutafova-Yambolieva, Violeta N.; Hwang, Sung Jin; Hao, Xuemei; Chen, Hui; Zhu, Michael X.; Wood, Jackie D.; Ward, Sean M.; Sanders, Kenton M.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.41 pp. 16359-16364
ISSN:
0027-8424
Subject:
adenine; adenosine triphosphate; agonists; antagonists; gastrointestinal system; muscles; nerve tissue; neurons; pyridoxal phosphate; receptors; smooth muscle; suramin; tetrodotoxin
Abstract:
... Peripheral inhibitory nerves are physiological regulators of the contractile behavior of visceral smooth muscles. One of the transmitters responsible for inhibitory neurotransmission has been reputed to be a purine, possibly ATP. However, the exact identity of this substance has never been verified. Here we show that β-nicotinamide adenine dinucleotide (β-NAD), an inhibitory neurotransmitter candi ...
DOI:
10.1073/pnas.0705510104
PubMed:
17913880
PubMed Central:
PMC2042211

28. B-vitamins, homocysteine and gene polymorphism in adults with fasting or post-methionine loading hyperhomocysteinemia

Author:
Cheng, Chien-Hsiung; Huang, Yi-Chia; Chen, Feng-Pan; Chou, Ming-Chih; Tsai, Tsung-Po
Source:
European journal of nutrition 2008 v.47 no.8 pp. 491-498
ISSN:
1436-6207
Subject:
adults; cross-sectional studies; fasting; folic acid; genetic polymorphism; genotype; homocysteine; methionine; methylenetetrahydrofolate reductase; pyridoxal phosphate
Abstract:
... Background Although fasting and post-methionine loading (PML) homocysteine concentrations are not necessarily related, a high percentage of hyperhomocysteinemia cases would be missed if methionine loading was not performed. Aim of the study The influences of B-vitamins and genetic polymorphism (methylenetetrahydrofolate reductase 677C [rightward arrow] T, MTHFR 677C [rightward arrow] T) on fasting ...
DOI:
10.1007/s00394-008-0752-5
PubMed:
18979127

29. Filling the gap of intracellular dephosphorylation in the Plasmodium falciparum vitamin B1 biosynthesis

Author:
Knöckel, Julia; Bergmann, Bärbel; Müller, Ingrid B.; Rathaur, Sushma; Walter, Rolf D.; Wrenger, Carsten
Source:
Molecular and biochemical parasitology 2008 v.157 no.2 pp. 241-243
ISSN:
0166-6851
Subject:
Northern blotting; Plasmodium falciparum; bacteria; biosynthesis; cytosol; dephosphorylation; enzymes; fungi; humans; malaria; nucleotides; parasites; pyridoxal phosphate; substrate specificity; sugars; thiamin; transfection
Abstract:
... Thiamine pyrophosphate (TPP), the active form of vitamin B1, is an essential cofactor for several enzymes. Humans depend exclusively on the uptake of vitamin B1, whereas bacteria, plants, fungi and the malaria parasite Plasmodium falciparum are able to synthesise thiamine monophosphate (TMP) de novo. TMP has to be dephosphorylated prior to pyrophosphorylation in order to obtain TPP. In P. falcipar ...
DOI:
10.1016/j.molbiopara.2007.10.010

30. Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and l-allo-Thr cleavage

Author:
Bhavani, B.S.; Rajaram, V.; Bisht, Shveta; Kaul, Purnima; Prakash, V.; Murthy, M.R.N.; Appaji Rao, N.; Savithri, H.S.
Source:
FEBS journal 2008 v.275 no.18 pp. 4606-4619
ISSN:
1742-464X
Subject:
Geobacillus stearothermophilus; X-radiation; glycine hydroxymethyltransferase; mutants; mutation; pyridoxal; pyridoxal phosphate; tetrahydrofolic acid; transamination; tyrosine
Abstract:
... Serine hydroxymethyltransferase (SHMT) from Bacillus stearothermophilus (bsSHMT) is a pyridoxal 5'-phosphate-dependent enzyme that catalyses the conversion of l-serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. In addition, the enzyme catalyses the tetrahydrofolate-independent cleavage of 3-hydroxy amino acids and transamination. In this article, we have examined the mech ...
DOI:
10.1111/j.1742-4658.2008.06603.x

31. Low Plasma Pyridoxal 5’-phosphate Concentration and MTHFR 677C→T Genotypes are Associated with Increased Risk of Hypertension

Author:
P.-T. Lin; C.-H. Cheng; J. C.-C. Wei; Y.-C. Huang
Source:
International journal for vitamin and nutrition research 2008 v.78 no.1 pp. 33-40
ISSN:
0300-9831
Subject:
vitamin B12; nutritional status; pyridoxal phosphate; blood chemistry; genetic polymorphism; alleles; hypertension; risk assessment; methylenetetrahydrofolate reductase; risk factors; genotype; folic acid; genetic variation; blood pressure; adults; homocysteine
Abstract:
... Few studies have linked homocysteine, B vitamins and/or genetic defects to the risk of hypertension. The purpose of this study was to investigate homocysteine, B-vitamins, and genetic mutation in relation to the risk of hypertension. Subjects were assigned to the hypertension (HTN) group (n = 50) or non-hypertension (non-HTN) group (n = 123). All subjects’ blood pressure (systolic blood pressure, ...
DOI:
10.1024/0300-9831.78.1.33

32. Preventing cell death induced by carbonyl stress, oxidative stress or mitochondrial toxins with vitamin B anti-AGE agents

Author:
Mehta, Rhea; Shangari, Nandita; O'Brien, Peter J.
Source:
Molecular nutrition & food research 2008 v.52 no.3 pp. 379-385
ISSN:
1613-4125
Subject:
glycation; cell death; lipid peroxidation; lipids; autoxidation; thiamin; oxidative stress; protective effect; carbohydrates; pyridoxine; toxins; animal disease models; chelating agents; copper; cytotoxicity; vitamin supplements; diabetes; acrolein; antioxidants; cyanides; pyridoxal phosphate; metabolic detoxification; beers
Abstract:
... Carbonyls generated by autoxidation of carbohydrates or lipid peroxidation have been implicated in advanced glycation end product (AGE) formation in tissues adversely affected by diabetes complications. Tissue AGE and associated pathology have been decreased by vitamin B₁/B₆ in trials involving diabetic animal models. To understand the molecular cytoprotective mechanisms involved, the effects of B ...
DOI:
10.1002/mnfr.200600190
PubMed:
17918169

33. Production of γ-aminobutyric acid by Streptococcus salivarius subsp. thermophilus Y2 under submerged fermentation

Author:
Yang, S.-Y.; Lü, F.-X.; Lu, Z.-X.; Bie, X.-M.; Jiao, Y.; Sun, L.-J.; Yu, B.
Source:
Amino acids 2008 v.34 no.3 pp. 473-478
ISSN:
0939-4451
Subject:
Streptococcus thermophilus; biotransformation; central nervous system; cheeses; drugs; functional foods; gamma-aminobutyric acid; glutamate decarboxylase; pH; pyridoxal phosphate; submerged fermentation; temperature; yogurt
Abstract:
... γ-Aminobutyric acid (GABA), a major inhibitory neurotransmitter in the central nervous system, has several well-known physiological functions and has been applied to the production of many drugs and functional foods. The technology of GABA production via submerged fermentation by Streptococcus salivarius subsp. thermophilus Y2 was investigated in this paper. It indicated that the GABA production w ...
DOI:
10.1007/s00726-007-0544-x

34. d-Amino acids in the brain: structure and function of pyridoxal phosphate-dependent amino acid racemases

Author:
Yoshimura, Tohru; Goto, Masaru
Source:
FEBS journal 2008 v.275 no.14 pp. 3527-3537
ISSN:
1742-464X
Subject:
Alzheimer disease; alanine; amyotrophic lateral sclerosis; brain; mammals; pyridoxal; pyridoxal phosphate; schizophrenia; serine
Abstract:
... d-Serine serves as a co-agonist of the N-methyl d-aspartate receptor in mammalian brains, and its behavior is probably related to neurological disorders such as schizophrenia, Alzheimer's disease and amyotrophic lateral sclerosis. d-Serine is synthesized by a pyridoxal 5'-phosphate (PLP)-dependent serine racemase. In this minireview, we provide a detailed discussion on the reaction mechanism of th ...
DOI:
10.1111/j.1742-4658.2008.06516.x

35. Catalytic and ligand-binding characteristics of Plasmodium falciparum serine hydroxymethyltransferase

Author:
Pang, Cullen K.T.; Hunter, Joshua H.; Gujjar, Ramesh; Podutoori, Ramulu; Bowman, Julie; Mudeppa, Devaraja G.; Rathod, Pradipsinh K.
Source:
Molecular and biochemical parasitology 2009 v.168 no.1 pp. 74-83
ISSN:
0166-6851
Subject:
Escherichia coli; Plasmodium falciparum; actin; biosynthesis; catalytic activity; dihydrofolate reductase; drugs; enzyme activity; enzyme substrates; genes; glycine hydroxymethyltransferase; histidine; malaria; messenger RNA; methionine; parasites; pyridoxal phosphate; pyrimethamine; serine; tetrahydrofolic acid; thymidine; thymidylate synthase
Abstract:
... The plant-like, bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) from malaria parasites has been a good target for drug development. Dihydrofolate reductase (DHFR) is inhibited by clinically established antimalarials, pyrimethamine and cycloguanil. Thymidylate synthase (TS) is the target of potent experimental antimalarials such as 5-fluoroorotate and 1843U89. Another enzyme in ...
DOI:
10.1016/j.molbiopara.2009.06.011
PubMed:
19591883
PubMed Central:
PMC2741015

36. Pyridoxal-5'-phosphate deficiency after intestinal and multivisceral transplantation

Author:
Matarese, Laura E.; Dvorchik, Igor; Costa, Guilherme; Bond, Geoffrey J.; Koritsky, Darlene A.; Ferraris, Ronaldo P.; Touger-Decker, Riva; O'Sullivan-Maillet, Julie K.; Abu-Elmagd, Kareem M.
Source:
American journal of clinical nutrition 2009 v.89 no.1 pp. 204-209
ISSN:
0002-9165
Subject:
adults; intestines; organ transplantation; pyridoxal phosphate; intestinal absorption; nutrient uptake; risk factors; postoperative complications; incidence; homocysteine; blood chemistry; parenteral feeding; enteral feeding; nutritional intervention; disease detection; nutrient deficiencies
Abstract:
... BACKGROUND: Successful intestinal transplantation is measured by the achievement of clinical nutritional autonomy (CNA). However, the ability of the graft to maintain normal micronutrient levels including vitamins has yet to be thoroughly evaluated. OBJECTIVE: After an initial clinical observation of isolated cases of pyridoxal-5'-phosphate (PLP) deficiency, this prospective study was designed to ...
DOI:
10.3945/ajcn.2008.26898
PubMed:
19056562

37. Utilization of an intron located polyadenlyation site resulted in four novel glutamate decarboxylase transcripts

Author:
Liu, Haixiong; Wang, Zhiqiang; Li, Shifeng; Zhang, Yunbin; Yan, Yuan-Chang; Li, Yi-ping
Source:
Molecular biology reports 2009 v.36 no.6 pp. 1469-1474
ISSN:
0301-4851
Subject:
testes; proteins; alternative splicing; pyridoxal phosphate; brain; gene expression; binding sites; rats; gamma-aminobutyric acid; molecular weight; introns; glutamate decarboxylase
Abstract:
... Glutamate decarboxylase (GAD) is the rate-limiting enzyme in the synthesis of γ-aminobutyric acid (GABA), the most important inhibitory neurotransmitter in central nervous system (CNS). Two homologous forms of GAD encoded by separate genes have been identified in mammalian brain, with molecular weight of 65 kDa (GAD65) and 67 kDa (GAD67). In the present study, four novel GAD67 transcripts produced ...
DOI:
10.1007/s11033-008-9337-x
PubMed:
18758993

38. Association of vitamin B-6 status with inflammation, oxidative stress, and chronic inflammatory conditions: the Boston Puerto Rican Health Study

Author:
Shen, Jian; Lai, Chao-Qiang; Mattei, Josiemer; Ordovas, Jose M.; Tucker, Katherine L.
Source:
American journal of clinical nutrition 2010 v.91 no.2 pp. 337-342
ISSN:
0002-9165
Subject:
DNA damage; diabetes mellitus; obesity; oxidative stress; C-reactive protein; dose response; glycohemoglobin; biomarkers; pyridoxine; blood plasma; chemical concentration; Puerto Ricans; metabolic syndrome; blood glucose; chronic diseases; human nutrition; islets of Langerhans; nutritional status; pyridoxal phosphate; inflammation; Puerto Rico; Massachusetts
Abstract:
... BACKGROUND: Low vitamin B-6 status has been linked to an increased risk of cardiovascular diseases. The cardioprotective effects of vitamin B-6 independent of homocysteine suggest that additional mechanisms may be involved. OBJECTIVE: Our objective was to examine the cross-sectional association of vitamin B-6 status with markers of inflammation and oxidative stress. DESIGN: We measured plasma pyri ...
Handle:
10113/39862
DOI:
10.3945/ajcn.2009.28571
PubMed:
19955400
PubMed Central:
PMC2806890

39. Dietary Patterns Are Associated with Hyperhomocysteinemia in an Urban Pakistani Population

Author:
Yakub, Mohsin; Iqbal, Mohammad Perwaiz; Iqbal, Romaina
Source:
Journal of nutrition 2010 v.140 no.7 pp. 1261-1266
ISSN:
0022-3166
Subject:
vitamin B12; folic acid; homocysteine; eating habits; women; raw vegetables; blood protein disorders; chicken meat; dietary surveys; plant source protein; human diseases; men; protein sources; human nutrition; food intake; animal source protein; pyridoxal phosphate; experimental diets; tea (beverage); red meat; urban population; Pakistan
Abstract:
... Little attention has been given to the association of dietary patterns with plasma homocysteine. Our objective in this study was to identify major dietary patterns and investigate their association with plasma homocysteine. In a cross-sectional survey, 872 healthy adults (355 males, 517 females; aged 18-60 y) were enrolled from an urban population in Karachi. Dietary intake was assessed by a FFQ. ...
DOI:
10.3945/jn.109.120477
PubMed:
20463142

40. Electrochemical probing into cytochrome c modification with homocysteine-thiolactone

Author:
Zhao, Jing; Zhu, Wei; Liu, Tao; Yang, Jinghua; Li, Genxi
Source:
Analytical and bioanalytical chemistry 2010 v.397 no.2 pp. 695-701
ISSN:
1618-2642
Subject:
correlation; cytochrome c; detection limit; electrochemistry; electrodes; electrospray ionization mass spectrometry; gold; heart; homocysteine; horses; pyridoxal phosphate; screening; thiols
Abstract:
... Homocysteine thiolactone modification is a unique process of posttranslational protein modification as well as a significant clinical indicator of cardiovascular and neurovascular diseases, so we report a new method in this paper to sensitively monitor such a modification using horse heart cytochrome c as a model protein. After the modification has been confirmed by UV-vis spectroscopy and ESI-MS, ...
DOI:
10.1007/s00216-010-3553-7

41. Impact of temperature on the time required for the establishment of primordial biochemistry, and for the evolution of enzymes

Author:
Stockbridge, Randy B.; Lewis, Charles A. Jr.; Yuan, Yang; Wolfenden, Richard
Source:
Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.51 pp. 22102-22105
ISSN:
0027-8424
Subject:
catalysts; enzymes; evolution; hydrolysis; phosphates; polysaccharides; pyridoxal phosphate; temperature
Abstract:
... All reactions are accelerated by an increase in temperature, but the magnitude of that effect on very slow reactions does not seem to have been fully appreciated. The hydrolysis of polysaccharides, for example, is accelerated 190,000-fold when the temperature is raised from 25 to 100 °C, while the rate of hydrolysis of phosphate monoester dianions increases 10,300,000-fold. Moreover, the slowest r ...
DOI:
10.1073/pnas.1013647107
PubMed:
21123742
PubMed Central:
PMC3009776

42. Discovery and structural characterization of a small molecule 14-3-3 protein-protein interaction inhibitor

Author:
Jing Zhao; Yuhong Du; John R. Horton; Anup K. Upadhyay; Bin Lou; Yan Bai; Xing Zhang; Lupei Du; Minyong Li; Binghe Wang; Lixin Zhang; Joseph T. Barbieri; Fadlo R. Khuri; Xiaodong Cheng; Haian Fu
Source:
Proceedings of the National Academy of Sciences of the United States of America 2011 v.108 no.39 pp. 16212-16216
ISSN:
0027-8424
Subject:
X-radiation; crystal structure; lysine; neurodegenerative diseases; protein-protein interactions; proteins; pyridoxal phosphate
Abstract:
... The 14-3-3 family of phosphoserine/threonine-recognition proteins engage multiple nodes in signaling networks that control diverse physiological and pathophysiological functions and have emerged as promising therapeutic targets for such diseases as cancer and neurodegenerative disorders. Thus, small molecule modulators of 14-3-3 are much needed agents for chemical biology investigations and therap ...
DOI:
10.1073/pnas.1100012108
PubMed:
21908710
PubMed Central:
PMC3182712

43. Endo-inulinase Stabilization by Pyridoxal Phosphate Modification: A Kinetics, Thermodynamics, and Simulation Approach

Author:
Homa Torabizadeh; Mehran Habibi-Rezaei; Mohammad Safari; Ali Akbar Moosavi-Movahedi; Ahmad Sharifizadeh; Homa Azizian; Massoud Amanlou
Source:
Applied biochemistry and biotechnology 2011 v.165 no.7-8 pp. 1661-1673
ISSN:
0273-2289
Subject:
energy; enzyme activity; half life; hydrolysis; inulin; inulinase; molecular dynamics; pyridoxal phosphate; substrate specificity; sucrose; temperature; thermal stability
Abstract:
... The structural and storage and functional thermostabilization of endo-inulinase (EC 3.2.1.7) through semi-rational modification of surface accessible lysine residues by pyridoxal-5′-phosphate (PLP) and ascorbate reduction have been explored. Improved stability was observed on modifications in the absence or presence of inulin, which indicates storage or functional thermostabilization, respectively ...
DOI:
10.1007/s12010-011-9385-x
PubMed:
21960273

44. Enzyme characteristics of aminotransferase FumI of Sphingopyxis sp. MTA144 for deamination of hydrolyzed fumonisin B1

Author:
Doris Hartinger; Heidi Schwartz; Christian Hametner; Gerd Schatzmayr; Dietmar Haltrich; Wulf-Dieter Moll
Source:
Applied microbiology and biotechnology 2011 v.91 no.3 pp. 757-768
ISSN:
0175-7598
Subject:
Escherichia coli; Sphingomonas; carboxylesterase; carcinogenicity; corn; deamination; enzyme activity; fumonisin B1; hydrolysis; multigene family; nuclear magnetic resonance spectroscopy; pH; pyridoxal phosphate; pyruvic acid; receptors; salt concentration; temperature
Abstract:
... Fumonisins are carcinogenic mycotoxins that are frequently found as natural contaminants in maize from warm climate regions around the world. The aminotransferase FumI is encoded as part of a gene cluster of Sphingopyxis sp. MTA144, which enables this bacterial strain to degrade fumonisin B1 and related fumonisins. FumI catalyzes the deamination of the first intermediate of the catabolic pathway, ...
DOI:
10.1007/s00253-011-3248-9
PubMed:
21503761
PubMed Central:
PMC3136697

45. Expression, in vivo localization and phylogenetic analysis of a pyridoxine 5′-phosphate oxidase in Arabidopsis thaliana

Author:
Yuying Sang; Robert D. Locy; Leslie R. Goertzen; Aaron M. Rashotte; Ying Si; Kwonkyoo Kang; Narendra K. Singh
Source:
Plant physiology and biochemistry 2011 v.49 no.1 pp. 88-95
ISSN:
0981-9428
Subject:
Arabidopsis thaliana; bioinformatics; chloroplasts; drought; ethylene; genes; heat stress; loci; monoclonal antibodies; mutants; oxidoreductases; phosphates; photolysis; phylogeny; protoplasts; pyridoxal phosphate; pyridoxamine; pyridoxine; root growth; sucrose
Abstract:
... Pyridoxal phosphate (PLP), a vitamin B₆ vitamer, is an essential cofactor for numerous enzymes. Pyridoxine/pyridoxamine phosphate oxidase (PPOX) catalyzes the synthesis of pyridoxal phosphate from pyridoxine phosphate (PNP) and/or pyridoxamine phosphate (PMP). The At5g49970 locus in Arabidopsis thaliana encodes an AtPPOX, a PNP/PMP oxidase. The expression of the AtPPOX gene varied in different tis ...
DOI:
10.1016/j.plaphy.2010.10.003

46. First cloning and characterization of aspartate aminotransferase from river buffalo (Bubalus bubalis)

Author:
Muhammad Shahid Nadeem; Naeem Rashid; Muzaffar Iqbal; Qurra-tul-Ann Afza Gardner; Muhammad Akhtar
Source:
Biologia 2011 v.66 no.6 pp. 1202-1210
ISSN:
1336-9563
Subject:
Escherichia coli; RNA; amino acid sequences; amino acids; aspartate transaminase; buffaloes; complementary DNA; cows; gene overexpression; genes; ionization; isozymes; pH; polypeptides; proteins; pyridoxal phosphate; reverse transcriptase polymerase chain reaction; sequence analysis; spectral analysis; temperature
Abstract:
... Aspartate aminotransferase catalyzes the transfer of an amino group from L-aspartate to α-oxoglutarate. We have purified cytosolic isozyme, to apparent homogeneity, from the heart of river buffalo. In order to clone the enzyme total RNA was isolated and the cDNA encoding complete polypeptide of 413 amino acids was amplified by reverse transcriptase polymerase chain reaction. The cDNA and the dedu ...
DOI:
10.2478/s11756-011-0125-z

47. Interactions between genetic variants of folate metabolism genes and lifestyle affect plasma homocysteine concentrations in the Boston Puerto Rican population

Author:
Huang, Tao; Tucker, Katherine L.; Lee, Yu-Chi; Crott, Jimmy W.; Parnell, Laurence D.; Shen, Jian; Smith, Caren E.; Ordovas, Jose M.; Li, Duo; Lai, Chao-Qiang
Source:
Public health nutrition 2011 v.14 no.10 pp. 1805-1812
ISSN:
1475-2727
Subject:
Puerto Ricans; alcoholic beverages; alleles; cross-sectional studies; drinking; folic acid; genetic polymorphism; genotype; homocysteine; interviews; lifestyle; men; metabolism; physical activity; pyridoxal phosphate; surveys; vitamin B12; women
Abstract:
... Objective To investigate genetic and lifestyle factors and their interactions on plasma homocysteine (Hcy) concentrations in the Boston Puerto Rican population. Design Cross-sectional study. Plasma concentrations of Hcy, folate, vitamin B12 and pyridoxal phosphate were measured, and genetic polymorphisms were determined. Data on lifestyle factors were collected in interviews. Setting A population ...
DOI:
10.1017/S1368980011000140
PubMed:
21338559
PubMed Central:
PMC4357314

48. Micronutrient Supplementation Improves Physical Performance Measures in Asian Indian School-Age Children

Author:
Mario Vaz; Maria Pauline; Uma S. Unni; Panam Parikh; Tinku Thomas; A.V. Bharathi; Sandhya Avadhany; Sumithra Muthayya; Ruchika Mehra; Anura V. Kurpad
Source:
Journal of nutrition 2011 v.141 no.11 pp. 2017-2023
ISSN:
0022-3166
Subject:
Asian Indians; ascorbic acid; beverages; dietary minerals; dietary supplements; exercise; exercise test; fatigue strength; folic acid; fortified foods; functional status; iron; niacin; nutritional status; placebos; pyridoxal phosphate; randomized clinical trials; riboflavin; school children; thiamin; vitamin B12
Abstract:
... Micronutrients are important in physical work capacity and therefore performance. The impact of a multi-micronutrient–fortified nutritional beverage on physical performance measures among clinically healthy school-age children was assessed in a double-blind (for test and placebo groups), placebo-controlled, randomized trial in children aged between 7 and 10.5 y (n = 300). The participants with hei ...
DOI:
10.3945/jn.110.135012
PubMed:
21918063

49. Suppression of isotope scrambling in cell-free protein synthesis by broadband inhibition of PLP enymes for selective ¹⁵N-labelling and production of perdeuterated proteins in H₂O

Author:
Su, Xun-Cheng; Loh, Choy-Theng; Qi, Ruhu; Otting, Gottfried
Source:
Journal of biomolecular NMR 2011 v.50 no.1 pp. 35-42
ISSN:
0925-2738
Subject:
Escherichia coli; cell-free protein synthesis; lysine; pyridoxal phosphate
Abstract:
... Selectively isotope labelled protein samples can be prepared in vivo or in vitro from selectively labelled amino acids but, in many cases, metabolic conversions between different amino acids result in isotope scrambling. The best results are obtained by cell-free protein synthesis, where metabolic enzymes are generally less active, but isotope scrambling can never be suppressed completely. We show ...
DOI:
10.1007/s10858-011-9477-5

50. A non-ACC pathway for ethylene biosynthesis in Botrytis cinerea

Author:
Altaf Qadir; Errol W. Hewett; Peter G. Long; David R. Dilley
Source:
Postharvest biology and technology 2011 v.62 no.3 pp. 314-318
ISSN:
0925-5214
Subject:
ethylene; 1-aminocyclopropane-1-carboxylic acid; cobalt; fruits; pyridoxal phosphate; aminocyclopropanecarboxylate oxidase; Botrytis cinerea; acids; chlorides; ethylene production; biochemical pathways; enzymatic reactions; methionine; kiwifruit
Abstract:
... Premature softening and tissue senescence occur in kiwifruit infected with Botrytis cinerea. While ethylene production is enhanced in infected fruit and B. cinerea produces ethylene on defined media in vitro the source of ethylene in this pathosystem is unclear. Ethylene production by B. cinerea was enhanced when methionine or ∝-keto-methylthiobutyric acid (KMBA) was added to a defined (modified ...
DOI:
10.1016/j.postharvbio.2011.06.003