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- Farshad Darvishi; Jacqueline Destain; Iraj Nahvi; Philippe Thonart; Hamid Zarkesh-Esfahani
- New biotechnology 2011 v.28 no.6 pp. 756-760
- Yarrowia lipolytica; bioreactors; carboxylic ester hydrolases; ethyl methanesulfonate; genes; hydrophobicity; molecular weight; mutants; mutation; oleic acid; open reading frames; pH; promoter regions; recombinant DNA; selective media; sequence analysis; temperature; value-added products; yeasts
- ... The yeast Yarrowia lipolytica degrades efficiently low-cost hydrophobic substrates for the production of various added-value products such as lipases. To obtain yeast strains producing high levels of extracellular lipase, Y. lipolytica DSM3286 was subjected to mutation using ethyl methanesulfonate (EMS) and ultraviolet (UV) light. Twenty mutants were selected out of 1600 mutants of Y. lipolytica t ...