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You searched for: Subject spectroscopy Remove constraint Subject: spectroscopy Subject biosynthesis Remove constraint Subject: biosynthesis Subject term spectroscopy Remove constraint Subject term: spectroscopy Subject term pyridoxal phosphate Remove constraint Subject term: pyridoxal phosphate
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1. The biosynthesis of ovothiol A (N1‐methyl‐4‐mercaptohistidine) : Identification of S‐(4′‐l‐histidyl)‐l‐cysteine sulfoxide as an intermediate and the products of the sulfoxide lyase reaction

Author:
Ryan N. Vogt; Hendrik S. C. Spies; Daniel J. Steenkamp
Source:
European journal of biochemistry 2001 v.268 no.20 pp. 5229-5241
ISSN:
0014-2956
Subject:
Crithidia fasciculata; NAD (coenzyme); NADH dehydrogenase; ammonium sulfate; anion exchange chromatography; biosynthesis; cysteine; dithiothreitol; high performance liquid chromatography; histidine; iron; isotope labeling; lactate dehydrogenase; oxygen; pyridoxal phosphate; pyruvic acid; spectral analysis; spectroscopy; sulfur; thiols
Abstract:
... Crude extracts of Crithidia fasciculata catalyse the formation of 4‐mercapto‐l‐histidine, an intermediate in the biosynthesis of ovothiol A (N1‐methyl‐4‐mercaptohistidine), in the presence of histidine, cysteine, Fe2+ and pyridoxal phosphate. This activity was present in a 35–55% ammonium sulfate fraction that was shown to produce a transsulfuration intermediate in the absence of pyr ...
DOI:
10.1046/j.0014-2956.2001.02444.x

2. Mechanistic enzymology of serine palmitoyltransferase

Author:
Hiroko Ikushiro; Hideyuki Hayashi
Source:
Biochimica et biophysica acta 2011 v.1814 no.11 pp. 1474-1480
ISSN:
1878-1454
Subject:
biosynthesis; pyridoxal phosphate; sphingolipids; spectroscopy; enzymology; serine C-palmitoyltransferase
Abstract:
... Serine palmitoyltransferase, which is one of the α-oxamine synthase family enzymes, catalyzes the condensation reaction of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine, the first and rate-determining step of the sphingolipid biosynthesis. As with other α-oxamine synthase family enzymes, the catalytic reaction is composed of multiple elementary steps, and the mechanism to control the ...
DOI:
10.1016/j.bbapap.2011.02.005