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You searched for: Subject term 4',6-diamidino-2-phenylindole Remove constraint Subject term: 4',6-diamidino-2-phenylindole
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1. Wound‐healing in Vaucheria longicaulis Hoppaugh var. macounii Blum.: 1. Cytomorphological study of the wound response

Author:
LAURIE TORNBOM; LUIS OLIVEIRA
Source:
new phytologist 1993 v.124 no.1 pp. 121-133
ISSN:
0028-646X
Subject:
4',6-diamidino-2-phenylindole; Vaucheria; chloroplasts; energy; exocytosis; fluorescence; light microscopy; lipids; mitochondria; plasmolysis; rhodamines; turgor
Abstract:
... The sequence of events leading to wound‐healing and resumption of growth are studied by light microscopy including the use of differential interference contrast optics in Vaucheria longicaulis Hoppaugh var. macounii Blum. Wound‐healing has been divided into four stages. In stage 1, a wound‐healing vesicle is rapidly extruded from the wound site. Loss of turgor pressure, as observed by the appearan ...
DOI:
10.1111/j.1469-8137.1993.tb03803.x

2. Evolutionary rate variation within Mus APRT

Author:
Fieldhouse, D.; Golding, G.B.
Source:
Genome = 1996 v.39 no.5 pp. 914-920
ISSN:
0831-2796
Subject:
fluorescence in situ hybridization; ribosomal RNA; Medicago sativa subsp. falcata; loci; chromosome banding; diploidy; ribosomal DNA; cytogenetic analysis; tetraploidy; indoles; nucleic acid hybridization; silver nitrate; fluorescent dyes
Abstract:
... Rodents are thought to have relatively high rates of evolution, twice as fast as the rates for mammals in other orders. However, the uniformly high rates of evolution inferred for the order Rodentia from Mus musculus and Rattus norvegicus are not consistently found for other rodent species. Using a maximum likelihood phylogenetic algorithm (DNAML), we show here that Mus spicilegus has a fivefold d ...
DOI:
10.1139/g96-115

3. The use of combined FISH/GISH in conjuction with DAPI counterstaining to identify chromosomes containing transgene inserts in amphidiploid tobacco. [Erratum: 1996, v. 105 (5), p. 231-236.]

Author:
Moscone, E.A.; Matzke, M.A.; Matzke, A.J.M.
Source:
Chromosoma 1996 v.105 no.4 pp. 231-236
ISSN:
0009-5915
Subject:
4',6-diamidino-2-phenylindole; Nicotiana tabacum; ancestry; cultivars; diploidy; fluorescence; fluorescence in situ hybridization; gene expression; loci; ribosomal DNA; tobacco; transgenes
Abstract:
... We have used combined fluorescent and genomic in situ hybridization (FISH/GISH) together with 4',6-diamidino-2-phenylindole (DAPI) counterstaining to determine simultaneously the chromosomal integration site and subgenomic allocation of a transgene insert in amphidiploid tobacco (Nicotiana tabacum, 2n = 4x = 48). The procedure provides sufficient information on physical markers to identify at leas ...
DOI:
10.1007/BF02528771

4. Anaerobic dechlorination of carbon tetrachloride by free-living and attached bacteria under various electron-donor conditions

Author:
Doong, R.A.; Chen, T.F.; Wu, Y.W.
Source:
Applied microbiology and biotechnology 1997 v.47 no.3 pp. 317-323
ISSN:
0175-7598
Subject:
4',6-diamidino-2-phenylindole; acetates; anaerobic conditions; bacteria; biodegradability; bioremediation; carbon tetrachloride; dechlorination; energy; glass; glucose; microbial activity; microscopy; porous media; thermodynamics
Abstract:
... The dechlorination of carbon tetrachloride (CCl4) by free-living and attached bacteria under anaerobic conditions was studied to examine the relationship between porous media and electron donor. Two batch-type experiments, the free-living and attached bacterial systems, were conducted with and without addition of 0.5-mm glass beads. Glucose and acetate were selected as the primary electron donors ...
DOI:
10.1007/s002530050934

5. Analysis of bacterial community structure in bulk soil by in situ hybridization

Author:
Zarda, B.; Hahn, D.; Chatzinotas, A.; Schonhuber, W.; Neef, A.; Amann, R.I.; Zeyer, J.
Source:
Archives of microbiology 1997 v.168 no.3 pp. 185-192
ISSN:
0302-8933
Subject:
4',6-diamidino-2-phenylindole; DNA; Gram-positive bacteria; Proteobacteria; bacterial communities; community structure; ethanol; fluorescence; homogenization; in situ hybridization; oligonucleotide probes; permeability; phylogeny; soil; soil sampling; ultrasonic treatment
Abstract:
... In situ hybridization with rRNA-targeted, fluorescent (Cy3-labeled) oligonucleotide probes was used to analyze bacterial community structure in ethanol- or paraformaldehyde-fixed bulk soil after homogenization of soil samples in 0.1% pyrophosphate by mild ultrasonic treatment. In ethanol-fixed samples 37 +/- 7%, and in paraformaldehyde 41 +/- 8% of the 4', 6-diamidino-2-phenylindole(DAPI)-stained ...
DOI:
10.1007/s002030050486

6. Stimulatory effect of growth hormone on in vitro maturation of bovine oocytes is exerted through cumulus cells and not mediated by IGF-I

Author:
Izadyar, F.; Tol, H.T.A. van.; Colenbrander, B.; Bevers, M.M.
Source:
Gamete research 1997 v.47 no.2 pp. 175-180
ISSN:
1040-452X
Subject:
4',6-diamidino-2-phenylindole; air; antibodies; carbon dioxide; cattle; complementary DNA; culture media; embryogenesis; granulosa cells; insulin-like growth factor I; messenger RNA; metaphase; oocytes; reverse transcriptase polymerase chain reaction; somatotropin; somatotropin receptors; staining
Abstract:
... A previous study reported that the addition of bovine growth hormone (bGH) during in vitro maturation of bovine oocytes accelerates nuclear maturation and stimulates subsequent embryonic development. The aim of this study was to investigate whether bovine cumulus oocyte complexes (COCs) contain growth hormone receptor (GHR), and whether the stimulatory effect of GH on oocyte maturation is cumulus- ...
DOI:
10.1002/(SICI)1098-2795(199706)47:2<175::AID-MRD8>3.0.CO;2-J
PubMed:
9136119

7. Use of immunofluorescence microscopy to compare small nuclei in two populations of Caulerpa taxifolia (Caulerpales, Chlorophyta)

Author:
Larry B. Liddle; Nelson Carvalho; Alexandre Meinesz
Source:
Phycologia 1998 v.37 no.4 pp. 310-312
ISSN:
2330-2968
Subject:
4',6-diamidino-2-phenylindole; Caulerpa taxifolia; confocal microscopy; fluorescence; fluorescence microscopy; life history; ploidy
Abstract:
... Nuclei from two populations of the siphonous green alga Caulerpa taxifolia (Vahl) C. Agardh were labeled with fluorescent antihistone antibodies and examined by confocal microscopy. Because the nuclei are small (2 μm diameter), it is difficult to study them by conventional methods, and DAPI staining does not distinguish the nuclei from intracellular bacteria. Using immunofluorescent microscopy, it ...
DOI:
10.2216/i0031-8884-37-4-310.1

8. Differential enumeration and in situ localization of microorganisms in the hindgut of the lower termite Mastotermes darwiniensis by hybridization with rRNA-targeted probes

Author:
Berchtold, M.; Chatzinotas, A.; Schonhuber, W.; Brune, A.; Amann, R.; Hahn, D.; Konig, H.
Source:
Archives of microbiology 1999 v.172 no.6 pp. 407-416
ISSN:
0302-8933
Subject:
4',6-diamidino-2-phenylindole; DNA; Gram-positive bacteria; Mastotermes darwiniensis; alpha-Proteobacteria; anaerobic conditions; biofilm; hindgut; in situ hybridization; microbial communities; microhabitats; oligonucleotide probes; oxygen; oxygen consumption; phylogeny; prokaryotic cells; sulfate-reducing bacteria
Abstract:
... We examined the abundance and spatial distribution of major phylogenetic groups of the domain Bacteria in hindguts of the Australian lower termite Mastotermes darwiniensis by using in situ hybridization with group-specific, fluorescently labeled, rRNA-targeted oligonucleotide probes. Between 32.0 +/- 7.2% and 52.3 +/- 8.2% of the DAPI-stained cells in different hindgut fractions were detected with ...
DOI:
10.1007/s002030050778

9. Culture-independent detection of changes in root-associated bacterial populations of common bean (phaseolus vulgaris L.) following nitrogen depletion

Author:
E. Schallmach; D. Minz; E. Jurkevitch
Source:
Microbial ecology 2000 v.40 no.4 pp. 309-316
ISSN:
0095-3628
Subject:
4',6-diamidino-2-phenylindole; Gram-positive bacteria; Phaseolus vulgaris; beans; branching; fluorescence; gel electrophoresis; nitrogen; polymerase chain reaction; rhizosphere; ribosomal RNA
Abstract:
... The structure of root-associated bacterial populations in the legume common bean (Phaseolus vulgaris L.), was studied in plants grown under nitrogen sufficiency and under conditions inducing nitrogen deficiency. Similar cell numbers were obtained in the rhizosphere of nitrogen-amended plants as compared to nitrogen-deficient plants and between various root parts—tip, elongation and branching zones ...
DOI:
10.1007/s002480000072

10. Susceptibility and Tolerance of New French Prunus domestica Cultivars to European Stone Fruit Yellows Phytoplasmas

Author:
W. Jarausch; J. P. Eyquard; M. Lansac; M. Mohns; F. Dosba
Source:
Phytopathologische Zeitschrift 2000 v.148 no.7-8 pp. 489-493
ISSN:
0931-1785
Subject:
4',6-diamidino-2-phenylindole; Phytoplasma; Prunus domestica; clones; cultivars; fruits; genotype; growth retardation; mortality; new variety; polymerase chain reaction; staining; stone fruits
Abstract:
... Five new cultivars of French prune (Prune d'Ente) and 11 cultivars or new clones of greengage (Reine Claude) were bud‐inoculated with European stone fruit yellows (ESFY) phytoplasmas and evaluated for their host response in the nursery over a period of 6 years. The ESFY infection was confirmed by specific polymerase chain reaction (PCR) detection and 4′,6‐diamidino‐2‐phenylindole (DAPI) staining i ...
DOI:
10.1046/j.1439-0434.2000.00535.x

11. Use of lycorine and DAPI staining in Saccharomyces cerevisiae to differentiate between rho0 and rho- cells in a cce1/deltacce1 nuclear background

Author:
Massardo, D.R.; Zweifel, S.G.; Gunge, N.; Miyakawa, I.; Sando, N.; Del Giudice, A.; Wolf, K.; Del Giudice, L.
Source:
Canadian journal of microbiology 2000 v.46 no.11 pp. 1058-1065
ISSN:
0008-4166
Subject:
Saccharomyces cerevisiae; lycorine; indoles; nucleases; mutants; fluorescent dyes; mitochondrial DNA; genes; genomics
DOI:
10.1139/w00-096
PubMed:
11109496

12. Bacterium-dinoflagellate interactions: investigative microscopy of Alexandrium spp. (Gonyaulacales, Dinophyceae)

Author:
Jane Lewis; Gay Kennaway; Susana Franca; Elsa Alverca
Source:
Phycologia 2001 v.40 no.3 pp. 280-285
ISSN:
2330-2968
Subject:
4',6-diamidino-2-phenylindole; fluorescence microscopy; lipopolysaccharides; sexual reproduction; toxins; transmission electron microscopy; vegetative growth
Abstract:
... The association of bacteria with dinoflagellates has been a neglected field of study, which has gained prominence in recent years because of the possible role of bacteria in toxin synthesis. A number of dinoflagellates undergo sexual reproduction, passing through various life-cycle stages in addition to the vegetative form. The presence of bacteria within dinoflagellates has been well established, ...
DOI:
10.2216/i0031-8884-40-3-280.1

13. Characterisation of pericentromeric and sticky intercalary heterochromatin in Ornithogalum longibracteatum (Hyacinthaceae)

Author:
Pedrosa, A.; Jantsch, M.F.; Moscone, E.A.; Ambros, P.F.; Schweizer, D.
Source:
Chromosoma 2001 v.110 no.3 pp. 203-213
ISSN:
0009-5915
Subject:
4',6-diamidino-2-phenylindole; DNA; Ornithogalum; centromeres; crossing over; fluorescence; heterochromatin; hexaploidy; histones; karyotyping; metaphase; mitosis; phosphorylation; prophase; satellite DNA
Abstract:
... The hexaploid liliaceous plant Ornithogalum longibracteatum (2n=6x=54) has a heterochromatin-rich bimodal karyotype with large (L) and small (S) chromosomes. The composition and subgenomic distribution of heterochromatin was studied using molecular and cytological methods. The major component of centromeric heterochromatin in all chromosomes is Sat1, an abundant satellite DNA with a basic repeat u ...
DOI:
10.1007/s004120000125

14. Cloning of DNA sequences localized on proximal fluorescent chromosome bands by microdissection in Pinus densiflora Sieb. & Zucc

Author:
Hizume, M.; Shibata, F.; Maruyama, Y.; Kondo, T.
Source:
Chromosoma 2001 v.110 no.5 pp. 345-351
ISSN:
0009-5915
Subject:
4',6-diamidino-2-phenylindole; DNA libraries; Pinus densiflora; chromosomes; clones; fluorescence; fluorescence in situ hybridization; genes; molecular cloning; oligonucleotides; polymerase chain reaction; ribosomal DNA; ribosomal RNA; tandem repeat sequences
Abstract:
... Japanese red pine, Pinus densiflora, has 2n=24 chromosomes, of which most carry chromomycin A3 (CMA) and 4',6-diamidino-2-phenylindole (DAPI) bands at their centromere-proximal regions. It was proposed that these regions contain highly repetitive DNA. The DNA localized in the proximal fluorescent bands was isolated and characterized. In P. densiflora, centromeric and neighboring segments of the so ...
DOI:
10.1007/s004120100149
PubMed:
11685534

15. Molecular cytogenetic studies in Chenopodium quinoa and Amaranthus caudatus

Author:
Jolanta Małuszyńska; Luz Gomez Pando; Bożena Kolano
Source:
Acta Societatis Botanicorum Poloniae 2001 v.70 no.2 pp. 85-90
ISSN:
0001-6977
Subject:
4',6-diamidino-2-phenylindole; Amaranthus caudatus; Chenopodium quinoa; cultivars; fluorescence; fluorescence in situ hybridization; hybridization; loci; ribosomal DNA; silver staining; South America
Abstract:
... Chenopodium quinoa Wild. and Amaranthus caudatus L., two plant species from South America, have small and numerous chromosomes. Looking for chromosome markers to distinguish pairs of homologous chromosomes double fluorescence staining, in situ hybridization with 45S rDNA and silver staining were applied. Fluorescent in situ hybridization with 45S rDNA has shown two sites of hybridization occurring ...
DOI:
10.5586/asbp.2001.011

16. New DAPI and FISH findings on egg maturation processes in related hybridogenetic and parthenogenetic Bacillus hybrids (Insecta, Phasmatodea)

Author:
Marescalchi, O.; Scali, V.
Source:
Gamete research 2001 v.60 no.2 pp. 270-276
ISSN:
1040-452X
Subject:
fluorescence in situ hybridization; parthenogenesis; hybrids; indoles; Phasmida; meiosis; nucleic acid hybridization; oocytes
DOI:
10.1002/mrd.1087
PubMed:
11553928

17. A tandemly repeated DNA sequence is associated with both knob-like heterochromatin and a highly decondensed structure in the meiotic pachytene chromosomes of rice

Author:
Cheng, Z.; Stupar, R.M.; Gu, M.; Jiang, J.
Source:
Chromosoma 2001 v.110 no.1 pp. 24-31
ISSN:
0009-5915
Subject:
4',6-diamidino-2-phenylindole; DNA; fluorescence in situ hybridization; heterochromatin; loci; meiosis; metaphase; nucleotide sequences; pachytene stage; rice; telomeres
Abstract:
... Highly repetitive tandem DNA sequence repeats are often associated with centromeric and telomeric regions of eukaryotic chromosomes. The rice tandem repeat Os48 is organized as long arrays of a 355 bp monomer and is mainly located in the telomeric regions. The chromosomal locations of the Os48 sequence were determined by fluorescence in situ hybridization (FISH) on rice pachytene chromosomes. The ...
DOI:
10.1007/s004120000126
PubMed:
11398973

18. Combining chlorination and chloramination processes for the inhibition of biofilm formation in drinking surface water system models

Author:
Momba, M.N.B.; Binda, M.A.
Source:
Journal of applied microbiology 2002 v.92 no.4 pp. 641-648
ISSN:
1364-5072
Subject:
4',6-diamidino-2-phenylindole; bacteria; bacterial adhesion; bacterial colonization; biofilm; chlorination; chlorine; disinfection; drinking water; fluorescence microscopy; microbial growth; models; rural communities; scanning electron microscopy; stainless steel; surface water; water treatment; Africa
Abstract:
... Aims: The aim of this study was to investigate the inhibition of biofilm formation on stainless steel (SS) and galvanized mild steel (MS) in chlorine (AFC1) and chlorine-monochloramine treated waters (AFC2M). Methods and Results: Disinfection was carried out using 2.5 mg l(-1) free chlorine followed by 1.5 mg l(-1) monochloramine, with non-disinfected water used as control water. Results of the st ...
DOI:
10.1046/j.1365-2672.2002.01556.x
PubMed:
11966904

19. Gibberellin produced in the cotyledon is required for cell division during tissue reunion in the Cortex of cut cucumber and tomato hypocotyls

Author:
Asahina, M.; Iwai, H.; Kikuchi, A.; Yamaguchi, S.; Kamiya, Y.; Kamada, H.; Satoh, S.
Source:
Plant physiology 2002 v.129 no.1 pp. 201-210
ISSN:
0032-0889
Subject:
tomatoes; cortex; biosynthesis; uniconazole; hypocotyls; Cucumis sativus; isotope labeling; cucumbers; gibberellic acid; cell division; transmission electron microscopy; mutants; dyes; indole acetic acid; Solanum lycopersicum var. lycopersicum
Abstract:
... Cucumber (Cucumis sativus) hypocotyls were cut to one-half of their diameter transversely, and morphological and histochemical analyses of the process of tissue reunion in the cortex were performed. Cell division in the cortex commenced 3 d after cutting, and the cortex was nearly fully united within 7 d. 4',6-Diamidino-2-phenylindole staining and 5-bromo-2'-deoxyuridine labeling experiments indic ...
PubMed:
12011351
PubMed Central:
PMC155884

20. In Situ Microspatial Imaging Using Two-Photon and Confocal Laser Scanning Microscopy of Bacteria and Extracellular Polymeric Secretions (EPS) Within Marine Stromatolites

Author:
Tomohiro Kawaguchi; Alan W. Decho
Source:
Marine biotechnology 2002 v.4 no.2 pp. 127-131
ISSN:
1436-2228
Subject:
4',6-diamidino-2-phenylindole; bacteria; confocal laser scanning microscopy; hydrophilicity; image analysis; sand; secretion
Abstract:
... The combination of a hydrophilic embedding resin, Nanoplast, with fluorescent probes, and subsequent imaging using two-photon and confocal laser scanning microscopy (2P-LSM and CLSM) has allowed in imaging of the in situ microspatial arrangements of microbial cells and their extracellular polymeric secretion (EPS) within marine stromatolites. Optical sectioning by 2P-LSM and CLSM allowed imaging o ...
DOI:
10.1007/s10126-001-0073-7