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- Liang, Ming-Hua; Jiang, Jian-Guo
- Algal research 2016 v.20 pp. 77-86
- algae, etc ; ATP citrate synthase; Dunaliella tertiolecta; Escherichia coli; acetyl coenzyme A; binding sites; biosynthesis; catalytic activity; complementary DNA; exons; fatty acids; flavonoids; genome walking; introns; nitrogen; phylogeny; reverse transcriptase polymerase chain reaction; transcription (genetics); transcription factors; Show all 19 Subjects
- ... ATP-citrate lyase (ACL) catalyzes the formation of cytosolic acetyl-CoA, which is responsible for the biosynthesis of fatty acids, lipids, and flavonoid. Here, cDNAs of the small and large subunits of ACL (DtACLA and DtACLB) and their genomic sequences were isolated from Dunaliella tertiolecta using RT-PCR, RACEs and genomic walking techniques. It was found that there were 12 exons and 11 introns ...