Jump to Main Content
- Takahashi, Yasuhiro, et al. Show all 7 Authors
- FEBS journal 2010 v.277 no.3 pp. 817-832
- Azotobacter vinelandii; Escherichia coli; anaerobic conditions; cysteine; electron paramagnetic resonance spectroscopy; iron; molecular weight; nitrogenase; operon; oxygen; reactive oxygen species; recombinant proteins; spectral analysis; sulfur; transactivators; transcription (genetics)
- ... Transcriptional activator VnfA is required for the expression of a second nitrogenase system encoded in the vnfH and vnfDGK operons in Azotobacter vinelandii. In the present study, we have purified full-length VnfA produced in E. coli as recombinant proteins (Strep-tag attached and tag-less proteins), enabling detailed characterization of VnfA for the first time. The EPR spectra of whole cells pro ...