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... Snake envenomation is of public health significance due to its hazardous effects on humans and animals. Mucuna pruriens (Fabaceae) is an established herbal drug and widespread in tropical and sub-tropical regions of the world. The seeds, bark and roots of medicinal plants like M. pruriens have been reported to antagonize the activity of some venoms and toxins. This study evaluated the in vitro ant ...
Mitchel Otieno Okumu; James Mucunu Mbaria; Joseph Kangangi Gikunju; Paul Gichohi Mbuthia; Vincent Odongo Madadi; Francis Okumu Ochola; Mercy Seroney Jepkorir
... Lethality and cytotoxicity assays of snake venoms and their neutralization by antivenom require many mice for the experiments. Recent developments have prompted researchers to seek alternative strategies that minimize the use of mice in line with Russel and Burch's 3Rs philosophy (Replacement, Reduction, and Refinement). Artemia salina is an animal model widely used for toxicity screening. However ...
Bitis arietans; L-amino-acid oxidase; Pseudechis; amino acids; bioactive compounds; chromatography; drug design; fractionation; geometry; lectins; mass spectrometry; medicinal properties; phospholipase A2; phospholipases; protein composition; protein structure; proteomics; snake venoms; snakes; therapeutics
Abstract:
... Snake venoms contain complex mixtures of proteins vital for the survival of venomous snakes. Aligned with their diverse pharmacological activities, the protein compositions of snake venoms are highly variable, and efforts to characterise the primary structures of such proteins are ongoing. Additionally, a significant knowledge gap exists in terms of the higher-order protein structures which modula ...
Bitis arietans; DNA; body condition; capital; dietary supplements; energy; females; males; paternity; radio telemetry; reproductive success; sexual selection
Abstract:
... Sexual selection studies often focus on morphological traits that are important only in the later stages of mate acquisition. Comparatively little is known about traits that lead to mate acquisition, such as mate-searching activities. We experimentally manipulated body condition (i.e., the energy reserves) in male puff adders (Bitis arietans) prior to the mating season using supplemental feeding i ...
Bitis arietans; DNA; body condition; body length; data collection; fecundity; females; males; mating systems; paternity; population; radio telemetry; reproductive success; sexual selection; snakes; sperm competition
Abstract:
... Body size is a key selected trait in many animal systems: larger size is sexually selected for in males because it confers a reproductive advantage during contest competition for access to females, and larger females are naturally selected for fecundity. Herein, we used radio‐telemetry to gather a large dataset of male–female interactions and DNA paternity analyses to characterize the intensity of ...
Bitis arietans; Naja; World Health Organization; ecosystems; farms; gardens; humans; irrigation; people; rain; snake bites; snakes; wildlife; Namibia
Abstract:
... Wildlife is known to be attracted to cities as a result of the ecosystem services associated with synanthorpization. This however often results in conflicts between human activities and wildlife. Accidental encounters between snakes and people in urban areas is a problem Windhoek shares with many cities around the world. Globally, this conflict leads to numerous human envenomations, a serious and ...
... Learning is a key behavioural adaptation allowing animals to respond to complex and changing environments. Although the field of animal behaviour has seen an increase in the taxonomic breadth of learning studies in recent decades, investigations within an explicit, broad phylogenetic framework are rare, curtailing our understanding of the evolution of learning and advanced cognition. Pit vipers (V ...
Paula Regina Simões de Oliveira; Felipe Silva de França; Isadora Maria Villas Boas; Marisa Maria Teixeira da Rocha; Sávio Stefanini Sant’Anna; Maria de Lourdes Bastos; Denise V. Tambourgi
Bitis arietans; Naja; accidents; antivenoms; blood serum; females; immunogenicity; males; morbidity; mortality; neutralization; provenance; public health; snake bites; snake venoms; snakes; therapeutics; toxicity; tropical diseases; Angola; South East Asia
Abstract:
... Snakebite is a public health problem in many countries of world. These accidents are considered a Neglected Tropical Disease and are responsible for a high morbidity and mortality index in the South and Southeast Asia and Sub-Saharan Africa. Angolan snake venoms are poorly investigated and no specific antivenom against them is available in the country. Thus, the aim of this study was to evaluate b ...
Bitis arietans; body condition; dietary supplements; food availability; food intake; foraging; home range; lifestyle; males; radio telemetry; snakes; video cameras; South Africa
Abstract:
... The effect of food availability on the spatial ecology of animals varies within and across study systems because a multitude of factors can affect the spatial activity of organisms. Low-energy specialists, such as ambush-foraging snakes, feed infrequently and can endure long periods without food. Because they have low-energy requirements, one possible tactic for feeding may be to simply ambush for ...
Bitis arietans; case studies; data collection; diet; ecology; gastrointestinal system; snakes
Abstract:
... : Examination of the gastrointestinal contents of museum specimens is routinely used as a method for assessing diet in a wide variety of reptiles. However, this method might be biased toward detecting prey items that are less digestible and larger in size because these food items are digested more slowly. In this study, we used fixed videography on free-ranging Puff Adders (Bitis arietans) as a co ...
David Gitirana da Rocha; Jorge Hernandez Fernandez; Claudia Maria Costa de Almeida; Claudia Letícia da Silva; Fabio Carlos Magnoli; Osmair Élder da Silva; Wilmar Dias da Silva
... The data presented in this article are related to the research article entitled "Development of IgY antibodies against anti-snake toxins endowed with highly lethal neutralizing activity" (da Rocha et al., 2017) [1]. Complementarity-determining region (CDR) sequences are variable antibody (Ab) sequences that respond with specificity, duration and strength to identify and bind to antigen (Ag) epitop ...
Andrés Sánchez; Álvaro Segura; Mariángela Vargas; María Herrera; Mauren Villalta; Ricardo Estrada; Francisco Wu; Thea Litschka-Koen; Michael Alfred Perry; Alberto Alape-Girón; Guillermo León
... EchiTAb-plus-ICP is an antivenom prepared from plasma of horses hyperimmunized with the venoms of the carpet viper (Echis ocellatus), the puff adder (Bitis arietans) and the black-necked spitting cobra (Naja nigricollis). Therefore, the use of this antivenom has been limited to Western Africa. In order to expand the neutralization scope of EchiTAb-plus-ICP, we supplemented the immunogenic mixture ...
Catherine A. Vulfius; Ekaterina N. Spirova; Marina V. Serebryakova; Irina V. Shelukhina; Denis S. Kudryavtsev; Elena V. Kryukova; Vladislav G. Starkov; Nina V. Kopylova; Maxim N. Zhmak; Igor A. Ivanov; Ksenia S. Kudryashova; Tatyana V. Andreeva; Victor I. Tsetlin; Yuri N. Utkin
... Phospholipase A2 (named bitanarin) possessing capability to block nicotinic acetylcholine receptors (nAChRs) was isolated earlier (Vulfius et al., 2011) from puff adder Bitis arietans venom. Further studies indicated that low molecular weight fractions of puff adder venom inhibit nAChRs as well. In this paper, we report on isolation from this venom and characterization of three novel peptides call ...
Bitis arietans; amphibians; birds; cognition; data collection; decision making; foraging; invertebrates; mimicry (behavior); models; predators; snakes; tail; South Africa
Abstract:
... Aggressive mimic species use signals typically resembling an attractive or harmless model to deceive other organisms in order to increase foraging success. With the exception of a few brood parasitic birds that combine two signals, most known cases of aggressive mimicry involve only a single signal. Here, we used fixed videography, a technique which consisted in setting up continuously recording v ...
... Two antivenoms prepared by using Echis ocellatus, Bitis arietans and Naja nigricollis venoms from different locations in sub-Saharan Africa were compared for their neutralizing ability. Both antivenoms were similarly effective in the neutralization of the venoms of the three species from different locations. However in the case of E. ocellatus venom, antivenom prepared using venom from Nigerian sp ...
Roberto T. Kodama; Daniela Cajado-Carvalho; Alexandre K. Kuniyoshi; Eduardo S. Kitano; Alexandre K. Tashima; Barbara F. Barna; Ana Carolina Takakura; Solange M.T. Serrano; Wilmar Dias-Da-Silva; Denise V. Tambourgi; Fernanda V. Portaro
Bitis arietans; angiotensin I; angiotensin-converting enzyme inhibitors; antihypertensive effect; bradykinin; hypotension; in vitro studies; in vivo studies; mass spectrometry; oligopeptides; peptidyl-dipeptidase A; snakes; synthetic peptides; tachycardia; venoms; Africa
Abstract:
... The snakes from the Bitis genus are some of the most medically important venomous snakes in sub Saharan Africa, however little is known about the composition and effects of these snake venom peptides. Considering that the victims with Bitis genus snakes have exacerbate hypotension and cardiovascular disorders, we investigated here the presence of angiotensin-converting enzyme modulators on four di ...
... Identification of snake species is important for various reasons including the emergency treatment of snake bite victims. We present a simple method for identification of six snake species using the gel filtration chromatographic profiles of their venoms. The venoms of Echis coloratus, Echis pyramidum, Cerastes gasperettii, Bitis arietans, Naja arabica, and Walterinnesia aegyptia were milked, lyop ...
... Extant disintegrins, as found in the venoms of Viperidae and Crotalidae snakes (vipers and rattlesnakes, represent a family of polypeptides that block the function of β1 and β3 integrin receptors, both potently and with a high degree of selectivity. This toxin family owes its origin to the neofunctionalization of the extracellular region of an ADAM (a disintegrin and metalloprotease) molecule recr ...
Marianne Molander; Line Nielsen; Søren Søgaard; Dan Staerk; Nina Rønsted; Drissa Diallo; Kusamba Zacharie Chifundera; Johannes van Staden; Anna K. Jäger
Bauhinia thonningii; Bitis arietans; Combretum; Grewia mollis; Lannea; Naja; Strychnos; active ingredients; antiserum; bioassays; enzyme inhibition; enzyme inhibitors; ethanol; hyaluronoglucosaminidase; inhibitory concentration 50; mortality; necrosis; patients; phospholipase A2; plant anatomy; polyphenols; proteinases; snake bites; snakes; traditional medicine; Democratic Republic of the Congo; Mali; South Africa
Abstract:
... Snakebite envenomation, every year, causes estimated 5–10,000 mortalities and results in more than 5–15,000 amputations in sub-Saharan Africa alone. Antiserum is not easily accessible in these regions or doctors are simply not available, thus more than 80% of all patients seek traditional practitioners as first-choice. Therefore it is important to investigate whether the plants used in traditional ...
... Mixed-species associations have been described in many vertebrate species, but few behavioral studies have investigated associations between species from different mammalian orders. Cape ground squirrels (Xerus inauris) are highly social rodents that inhabit burrows with two species of mongoose, but the benefits of these interspecific associations to ground squirrels remain unresolved. We compared ...