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You searched for: Subject term Brassica napus Remove constraint Subject term: Brassica napus Subject term beta-glucuronidase Remove constraint Subject term: beta-glucuronidase
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1. Temporary inhibition of cell wall synthesis improves the transient expression of the GUS gene in Brassica napus mesophyll protoplasts

Author:
Chapel, M.; Glimelius, K.
Source:
Plant cell reports 1990 v.9 no.2 pp. 105-108
ISSN:
0721-7714
Subject:
Brassica napus; mesophyll; protoplasts; genetic transformation; gene expression; cell walls; biosynthesis; beta-glucuronidase; enzyme activity; electroporation
DOI:
10.1007/BF00231560
PubMed:
24226441

2. Electroporation of rapeseed protoplasts – transient and stable transformation

Author:
Per Bergman; Kristina Glimelius
Source:
Physiologia plantarum 1993 v.88 no.4 pp. 604-611
ISSN:
0031-9317
Subject:
Brassica napus; X-radiation; antibiotic resistance; beta-glucuronidase; callus; electroporation; gene transfer; genes; genetic markers; hypocotyls; irradiation; kanamycin kinase; neomycin; plasmids; protoplasts; rapeseed; transgenic plants
Abstract:
... Protoplasts of Brassica napus hypocotyls were transfected using electroporation. Parameters such as discharge potential, protoplast density and buffer constituents were tested to determine the most suitable conditions for gene transfer. To monitor the introduction of DNA into protoplasts a plasmid containing the β‐glucuronidase (EC 3.2.1.31), and the neomycin phospotransferase (EC 2.7.1.95) genes ...
DOI:
10.1111/j.1399-3054.1993.tb01378.x

3. A combined use of microprojectile bombardment and DNA imbibition enhances transformation frequency of canola (Brassica napus L.)

Author:
Chen, J.L.; Beversdorf, W.D.
Source:
Theoretical and applied genetics 1994 v.88 no.2 pp. 187-192
ISSN:
0040-5752
Subject:
Brassica napus; genetic transformation; gene transfer; direct DNA uptake; imbibition; reporter genes; beta-glucuronidase; phosphotransferases (phosphomutases); transgenic plants
Abstract:
... Efforts to increase the frequency of recovered homozygous transgenic B. napus plants from direct DNA transformation treatments led to the development of a method of combined microprojectile bombardment and desiccation/DNA imbibition. The combined method was compared to individual treatments in two experiments utilizing microspore-derived embryo hypocotyls as targets for the beta-glucuronidase (GUS ...
DOI:
10.1007/BF00225896
PubMed:
24185925

4. Regulation of an Arabidopsis oleosin gene promoter in transgenic Brassica napus

Author:
Plant, A.L.; Van Rooijen, G.J.H.; Anderson, C.P.; Moloney, M.M.
Source:
Plant molecular biology 1994 v.25 no.2 pp. 193-205
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; Brassica napus; plant proteins; lipid bodies; recombinant DNA; beta-glucuronidase; reporter genes; transgenic plants; gene expression; abscisic acid; jasmonic acid; sorbitol; embryo (plant); embryogenesis; promoter regions
Abstract:
... Progressive deletions of the 5'-flanking sequences of an Arabidopsis oleosin gene were fused to beta-glucuronidase (GUS) and introduced into Brassica napus plants using Agrobacterium-mediated transformation. The effect of these deletions on the quantitative level of gene expression, organ specificity and developmental regulation was assessed. In addition, the influence of abscisic acid (ABA), jasm ...
DOI:
10.1007/BF00023237
PubMed:
8018869

5. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

6. Transient expression and oil body targeting of an Arabidopsis oleosin-GUS reporter fusion protein in a range of oilseed embryos

Author:
Abenes, M.; Holbrook, L.; Moloney, M.
Source:
Plant cell reports 1997 v.17 no.1 pp. 1-7
ISSN:
0721-7714
Subject:
Brassica napus; gene transfer; Arabidopsis thaliana; embryo (plant); binding proteins; beta-glucuronidase; enzyme activity; reporter genes; gene expression; developmental stages; histochemistry; transgenic plants
Abstract:
... An Arabidopsis oleosin gene including 860 bp of promoter as well as coding sequence was fused to the reporter, beta-glucuronidase (GUS). In transient expression assays following particle bombardment, the Arabidopsis gene in both transcriptional and translational fusions with GUS was functional in a range of oilseed embryos as determined histochemically and fluorimetrically. Significantly, upon hom ...
DOI:
10.1007/s002990050341

7. Analyses in transgenic tobacco of the promoter from a Brassica napus gene highly expressed in the stigma

Author:
Robert, L.S.; Levesque-Lemay, M.; Gerster, J.L.; Hong, H.P.; Keller, W.
Source:
Plant cell reports 1999 v.18 no.5 pp. 357-362
ISSN:
0721-7714
Subject:
Arabidopsis thaliana; genetic transformation; gene transfer; transgenic plants; Agrobacterium radiobacter; developmental stages; clones; gene expression; stigma; amino acid sequences; Nicotiana tabacum; genes; Brassica napus; nucleotide sequences; enzyme activity; histochemistry; beta-glucuronidase
Abstract:
... A genomic clone, Pis G363, containing the Brassica napus stigma-expressed gene Pis 63-2 was isolated and sequenced. The coding region of Pis G363 does not possess introns and shows 82% identity to the nucleotide sequence of a gene from Arabidopsis BAC clone T01B08. A 2-kb promoter fragment from Pis G363 was fused to the coding sequence of the marker enzyme beta-glucuronidase (GUS) and introduced i ...
DOI:
10.1007/s002990050586

8. A Brassica napus skp1-like gene promoter drives GUS expression in Arabidopsis thaliana male and female gametophytes

Author:
Drouaud, J.; Marrocco, K.; Ridel, C.; Pelletier, G.; Guerche, P.
Source:
Sexual plant reproduction 2000 v.13 no.1 pp. 29-35
ISSN:
0934-0882
Subject:
Arabidopsis thaliana; mitosis; spores; transgenic plants; developmental stages; polymerase chain reaction; amino acid sequences; gene expression; Brassica napus; promoter regions; beta-glucuronidase; plant proteins
DOI:
10.1007/PL00009839

9. NEC1, a novel gene, highly expressed in nectary tissue of Petunia hybrida

Author:
Ge, Y.X.; Angenent, G.C.; Wittich, P.E.; Peters, J.; Franken, J.; Busscher, M.; Zhang, L.M.; Dahlhaus, E.; Kater, M.M.; Wullems, G.J.; Creemers-Molenaar, T.
Source:
plant journal 2000 v.24 no.6 pp. 725-734
ISSN:
0960-7412
Subject:
nectar; secretion; cell membranes; Brassica napus; plant proteins; phenotype; nectaries; starch; gene expression; cytoplasm; leaves; anthers; polymerase chain reaction; amino acid sequences; phloem; Petunia hybrida; transgenic plants; nucleotide sequences; reporter genes; beta-glucuronidase
DOI:
10.1046/j.1365-313x.2000.00926.x
PubMed:
11135107

10. Analysis of a backcross population from a multi-copy transgenic Brassica napus line

Author:
Bavage, A.D.; Buck, E.; Dale, P.; Moyes, C.; Senior, I.
Source:
Euphytica 2002 v.124 no.3 pp. 333-340
ISSN:
0014-2336
Subject:
Brassica napus; transgenic plants; gene dosage; gene expression; beta-glucuronidase; reporter genes; gene interaction; backcrossing

11. Ethanol vapor is an efficient inducer of the alc gene expression system in model and crop plant species

Author:
Sweetman, J.P.; Chu, C.; Qu, N.; Greenland, A.J.; Sonnewald, U.; Jepson, I.
Source:
Plant physiology 2002 v.129 no.3 pp. 943-948
ISSN:
0032-0889
Subject:
alcohol dehydrogenase; chemical constituents of plants; transgenic plants; recombinant proteins; starch crops; Solanum tuberosum; gene expression; potatoes; Nicotiana tabacum; leaves; Brassica napus; reporter genes; vapors; plant morphology; ethanol; beta-glucuronidase; tobacco; plant proteins
Abstract:
... We have demonstrated that low concentrations of ethanol vapor efficiently induce the alc gene expression system in tobacco (Nicotiana tabacum cv Samsun NN), potato (Solanum tuberosum cv Solara), and oilseed rape (Brassica napus cv Westar). For many situations, this may be the preferred method of induction because it avoids direct application of comparatively high concentrations of an ethanol solut ...
PubMed:
12114549
PubMed Central:
PMC1540238

12. Functional significance of the alternative transcript processing of the Arabidopsis floral promoter FCA

Author:
Macknight, R.; Duroux, M.; Laurie, R.; Dijkwel, P.; Simpson, G.; Dean, C.
Source:
plant cell 2002 v.14 no.4 pp. 877-888
ISSN:
1040-4651
Subject:
Arabidopsis thaliana; messenger RNA; flowering; alternative splicing; buds; transgenic plants; flowers; binding proteins; reporter genes; introns; roots; beta-glucuronidase; shoots
Abstract:
... The Arabidopsis gene FCA encodes an RNA binding protein that functions to promote the floral transition. The FCA transcript is alternatively processed to yield four transcripts, the most abundant of which is polyadenylated within intron 3. We have analyzed the role of the alternative processing on the floral transition. The introduction of FCA intron-less transgenes resulted in increased FCA prote ...
PubMed:
11971142
PubMed Central:
PMC150689

13. T-DNA tagging of a pathogen inducible promoter in Arabidopsis thaliana

Author:
Custers, J.H.H.V.; Melchers, L.S.; Tigelaar, H.; Bade, J.B.; Spiegeler, J.J.M.; Meijs, P.J. van der.; Simons, B.H.; Stuiver, M.H.
Source:
Molecular plant pathology 2002 v.3 no.4 pp. 239-249
ISSN:
1464-6722
Subject:
Arabidopsis thaliana; open reading frames; Botrytis cinerea; transgenic plants; amino acid sequences; binding sites; reporter genes; Brassica napus; linkage (genetics); promoter regions; beta-glucuronidase; infection; defense mechanisms
DOI:
10.1046/j.1364-3703.2002.00115.x
PubMed:
20569331

14. Temporal and spatial expression of a polygalacturonase during leaf and flower abscission in oilseed rape and Arabidopsis

Author:
Gonzalez-Carranza, Z.H.; Whitelaw, C.A.; Swarup, R.; Roberts, J.A.
Source:
Plant physiology 2002 v.128 no.2 pp. 534-543
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; temporal variation; abscission; complementary DNA; animal proteins; polymerase chain reaction; flowers; amino acid sequences; gene expression; Scyphozoa; spatial variation; Brassica napus; reporter genes; promoter regions; beta-glucuronidase; leaves
Abstract:
... During leaf abscission in oilseed rape (Brassica napus), cell wall degradation is brought about by the action of several hydrolytic enzymes. One of these is thought to be polygalacturonase (PG). Degenerate primers were used to isolate a PG cDNA fragment by reverse transcriptase-polymerase chain reaction from RNA extracted from ethylene-promoted leaf abscission zones (AZs), and in turn a full-lengt ...
DOI:
10.1104/pp.010610
PubMed:
11842157
PubMed Central:
PMC148916

15. Development of a novel Agrobacterium-mediated transformation method to recover transgenic Brassica napus plants

Author:
Wang, W.C.; Menon, G.; Hansen, G.
Source:
Plant cell reports 2003 v.22 no.4 pp. 274-281
ISSN:
0721-7714
Subject:
developmental stages; oil crops; plant proteins; Agrobacterium radiobacter; gene expression; phenotypic variation; Arabidopsis thaliana; canola; seeds; herbicide resistance; Brassica napus var. napus; genetic transformation; transgenes; experimental design; transgenic plants; oxidoreductases; nucleotide sequences; reporter genes; plant morphology; genetic vectors; beta-glucuronidase
Abstract:
... We report here an in planta method to produce transgenic Brassica napus plants. The procedure included Agrobacterium-mediated inoculation of plants at various development stages along with a vacuum infiltration step. The flowering stage appeared to be the most receptive stage for transformation and production of transgenic plants. In some cases, the flowering stage was induced either by cold treat ...
DOI:
10.1007/s00299-003-0691-9
PubMed:
14586552

16. Evaluation of putative seed-specific promoters for Linum usitatissimum

Author:
Drexler, H.H.S.; Scheffler, J.A.; Heinz, E.
Source:
Molecular breeding 2003 v.11 no.2 pp. 149-158
ISSN:
1380-3743
Subject:
Linum usitatissimum; flax; transgenic plants; genetic transformation; gene transfer; long-chain-fatty-acid-CoA ligase; Brassica napus; Vicia faba; legumin; reporter genes; transgenes; beta-glucuronidase; gene expression; seed development; leaves; roots
Abstract:
... The GUS reporter gene was used to test four different putative seed-specific promoters in developing and mature seeds, leaves and roots from linseed flax (Linum usitatissimum). The promoters tested included the regulatory regions of the beta-ketoacyl-CoA synthase gene (KCS) and the napin protein gene from Brassica napus, the promoter regions of the 'unknown seed protein' (USP), and a legumin prote ...

17. High frequency shoot regeneration and Agrobacterium-mediated DNA transfer in canola (Brassica napus)

Author:
Khan, M.R.; Rashid, H.; Ansar, M.; Chaudry, Z.
Source:
Plant cell, tissue, and organ culture 2003 v.75 no.3 pp. 223-231
ISSN:
0167-6857
Subject:
oil crops; benzyladenine; genes; Brassica napus; 2,4-D; shoots; dose response; hypocotyls; Agrobacterium radiobacter; gibberellic acid; explants; varieties; naphthaleneacetic acid; in vitro regeneration; enzyme activity; culture media; genetic transformation; gene transfer; transgenic plants; silver nitrate; beta-glucuronidase
Abstract:
... Five different varieties of Brassica napus (Cyclone, Dunkled, Oscar, Rainbow and KS75) were tested for their regeneration response. Cyclone showed a very high frequency of regeneration (92%). The use of silver nitrate was a pre-requisite for efficient shoot regeneration. Hypocotyls were selected as the starting material for transformation experiments on the basis of high transient GUS expression. ...

18. T-DNA tagging in Brassica napus as an efficient tool for the isolation of new promoters for selectable marker genes

Author:
Bade, J.; Van Grinsven, E.; Custers, J.; Hoekstra, S.; Ponstein, A.
Source:
Plant molecular biology 2003 v.52 no.1 pp. 53-68
ISSN:
0167-4412
Subject:
genetic transformation; antibiotic resistance; stems; kanamycin kinase; transgenic plants; DNA; Agrobacterium radiobacter; Solanum tuberosum; plasmid vectors; hypocotyls; potatoes; Brassica napus; genetic techniques and protocols; reporter genes; promoter regions; beta-glucuronidase; callus
Abstract:
... A simple strategy to identify and isolate new promoters suitable for driving the expression of selectable marker genes is described. By employing a Brassica napus hypocotyl transformation protocol and a promoterless gus::nptII tagging construct, a series of 20 kanamycin-resistant tagged lines was produced. Most of the regenerated plants showed hardly any GUS activity in leaf, stem and root tissues ...
PubMed:
12825689

19. A ROS repressor-mediated binary regulation system for control of gene expression in transgenic plants

Author:
Schafer, U.A.; Hegedus, D.D.; Bate, N.J.; Hannoufa, A.
Source:
Transgenic research 2004 v.13 no.2 pp. 109-118
ISSN:
0962-8819
Subject:
Arabidopsis thaliana; genetic transformation; transgenes; transgenic plants; Agrobacterium radiobacter; recombinant fusion proteins; operator regions; synthetic genes; bacterial proteins; DNA-binding proteins; Brassica napus; binding sites; gene expression regulation; promoter regions; beta-glucuronidase; rapeseed
DOI:
10.1023/B:TRAG.0000026058.84385.5b
PubMed:
15198199

20. Cell specific, cross-species expression of myrosinases in Brassica napus, Arabidopsis thaliana and Nicotiana tabacum

Author:
Thangstad, O.P.; Gilde, B.; Chadchawan, S.; Seem, M.; Husebye, H.; Bradley, D.; Bones, A.M.
Source:
Plant molecular biology 2004 v.54 no.4 pp. 597-611
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; guard cells; recombinant DNA; transgenic plants; embryo (plant); gene expression; phloem; Nicotiana tabacum; Brassica napus; thioglucosidase; reporter genes; tobacco; enzyme activity; promoter regions; beta-glucuronidase; rapeseed
Abstract:
... A prototypical characteristic of the Brassicaceae is the presence of the myrosinase-glucosinolate system. Myrosinase, the only known S-glycosidase in plants, degrades glucosinolates, thereby initiating the formation of isothiocyanates, nitriles and other reactive products with biological activities. We have used myrosinase gene promoters from Brassica napus and Arabidopsis thaliana fused to the β- ...
PubMed:
15316292