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You searched for: Subject term Butyrivibrio fibrisolvens Remove constraint Subject term: Butyrivibrio fibrisolvens
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1. Proteolytic activity of the ruminal bacterium Butyrivibrio fibrisolvens

Author:
Cotta, M.A.; Hespell, R.B.
Source:
Applied and environmental microbiology 1986 v.52 no.1 pp. 51-58
ISSN:
0099-2240
Subject:
Butyrivibrio fibrisolvens; strains; rumen bacteria; proteolysis; microbial activity
Abstract:
... The proteolytic activity of Butyrivibrio fibrisolvens, a ubiquitously distributed bacterial species in the gastrointestinal tracts of ruminants and other mammals, was characterized. The relative proteolytic activity (micrograms of azocasein degraded per hour per milligram of protein) varied greatly with the strain: 0 to 1 for strains D1, D16f, E21C, and X6C61; 7 to 15 for strains IL631, NOR37, S2, ...
Handle:
10113/24008
PubMed:
3524460
PubMed Central:
PMC203391

2. Fermentation of xylans by Butyrivibrio fibrisolvens and other ruminal bacteria

Author:
Hespell, R.B.; Wolf, R.; Bothast, R.J.
Source:
Applied and Environmental microbiology 1987 v.53 no.12 pp. 2849-2853
ISSN:
0099-2240
Subject:
Butyrivibrio fibrisolvens; rumen bacteria; enzyme activity; wheat straw; oats; wood; cell growth; enzymes
Abstract:
... The ability of Butyrivibrio fibrisolvens and other ruminal bacteria (6 species, 18 strains) to ferment a crude xylan from wheat straw or to ferment xylans from larchwood or oat spelts was studied. Liquid cultures were monitored for carbohydrate utilization, cell growth (protein), and fermentation acid production. B. fibrisolvens 49, H17c, AcTF2, and D1 grew almost as well on one or more of the xyl ...
Handle:
10113/23818
PubMed:
3124741
PubMed Central:
PMC204211

3. Esterase activities in Butyrivibrio fibrisolvens strains

Author:
Hespell, R.B.; O'Bryan-Shah, P.J.
Source:
Applied and environmental microbiology 1988 v.54 no.8 pp. 1917-1922
ISSN:
0099-2240
Subject:
Butyrivibrio fibrisolvens; strains; esterases; enzyme activity; rumen bacteria
Abstract:
... Thirty strains of Butyrivibrio fibrisolvens isolated in diverse geographical locations were examined for esterase activity by using naphthyl esters of acetate, butyrate, caprylate, laurate, and palmitate. All strains possessed some esterase activity, and high levels of activity were observed with strains 49, H17c, S2, AcTF2, and LM8/1B. Esterase activity also was detected in other ruminal bacteria ...
Handle:
10113/23880
PubMed:
3178205
PubMed Central:
PMC202779

4. Identification of L-iduronic acid as a constituent of the major extracellular polysaccharide produced by Butyrivibrio fibrisolvens strain X6C61

Author:
Stack, R.J.; Plattner, R.D.; Cote, G.L.
Source:
FEMS microbiology letters 1988 v.51 no.1 pp. 1-6
ISSN:
0378-1097
Subject:
Butyrivibrio fibrisolvens; rumen bacteria; polysaccharides; uronic acids
Abstract:
... Butyrivibrio fibrisolvens strain X6C61 produces two extracellular polysaccharides (EPS-I and EPS- II) separable by anion-exchange chromatography. The neutral sugar constituents of EPS-I were identified by gas-liquid chromatography (GLC) as the alditol acetates of rhamnose, mannose, galactose, glucose, and an unidentified component. These results were confirmed using thin-layer chromatography (TLC) ...
Handle:
10113/24326

5. Isolation and characterization of Xylan-degrading strains of Butyrivibrio fibrisolvens from a Napier grass-fed anaerobic digester

Author:
Sewell, G.W.; Aldrich, H.C.; Williams, D.; Mannarelli, B.; Wilkie, A.; Hespell, R.B.; Smith, P.H.; Ingram, L.O.
Source:
Applied and Environmental microbiology 1988 v.54 no.5 pp. 1085-1090
ISSN:
0099-2240
Subject:
anaerobic conditions; xylan; Butyrivibrio fibrisolvens; digestion; Cenchrus purpureus; strains; enzyme activity
Abstract:
... Abstract: Six new xylanolytic bacterial strains have been isolated from a Napier grass-fed anaerobic digester. These strains were identified as Butyrivibrio fibrisolvens and were similar in many respects to ruminal isolates described previously. The new isolates exhibited a high degree of DNA homology with several ruminal strains of B. fibrisolvens. Xylan or xylose was required to induce to produc ...
Handle:
10113/23864
PubMed:
16347622

6. Neutral sugar composition of extracellular polysaccharides produced by strains of Butyrivibrio fibrisolvens

Author:
Stack, R.J.
Source:
Applied and Environmental microbiology 1988 v.54 no.4 pp. 878-883
ISSN:
0099-2240
Subject:
rumen bacteria; Butyrivibrio fibrisolvens; polysaccharides
Abstract:
... Astract: The extracellular polysaccharides (EPSs) produced by 37 isolates presently classified as Butyrivibrio species (or more specifically as Butyrivibrio fibrisolvens) were purified from glucose-grown cultures. The neutral sugar compositions of these EPSs were determined by both thin-layer and gas-liquid chromatographic techniques. Results showed that while the neutral sugar composition of the ...
Handle:
10113/23859
PubMed:
3377501
PubMed Central:
PMC202567

7. Cloning, sequencing, and expression of a xylanase gene from the anaerobic ruminal bacterium Butyrivibrio fibrisolvens

Author:
Mannarelli, B.M.; Evans, S.; Lee, D.
Source:
Journal of bacteriology 1990 v.172 no.8 pp. 4247-4254
ISSN:
0021-9193
Subject:
Butyrivibrio fibrisolvens; rumen bacteria; genes; gene expression; enzymes; enzyme activity; Escherichia coli; nucleotide sequences
Abstract:
... A gene coding for xylanase activity, xynA, from the anaerobic ruminal bacterium Butyrivibrio fibrisolvens 49 was cloned into Escherichia coli JM83 by using plasmid pUC19. The gene was located on a 2.3-kilobase (kb) DNA insert composed of two adjacent EcoRI fragments of 1.65 and 0.65 kb. Expression of xylanase activity required parts of both EcoRI segments. In E. coli, the cloned xylanase enzyme wa ...
Handle:
10113/24260
PubMed:
2198249
PubMed Central:
PMC213248

8. Conjugal transfer of Tn916, Tn916 delta E, and pAM beta 1 from Enterococcus faecalis to Butyrivibrio fibrisolvens strains

Author:
Hespell, R.B. Research Service, U.S. Department of Agriculture, Peoria, IL.; Whitehead, T.R.
Source:
Applied and environmental microbiology 1991 v.57 no.9 pp. 2703-2709
ISSN:
0099-2240
Subject:
rumen bacteria; Butyrivibrio fibrisolvens; strains; Enterococcus faecalis; transposons; plasmids; genes
Abstract:
... Anaerobic filter matings of Butyrivibrio fibrisolvens H17c, CF3, D1, or GS113, representing different DNA relatedness groups, were done with Enterococcus faecalis CG11O, which contains chromosomally inserted Tn916. Tetracycline-resistant transconjugants were obtained with each mating pair at average frequencies of 4.4 x 10(-6) (per recipient) and 5.2 X 10(-6) (per donor). The transfer frequencies ...
Handle:
10113/37289
PubMed:
1662939
PubMed Central:
PMC183644

9. Some chemical and physical properties of extracellular polysaccharides produced by Butyrivibrio fibrisolvens strains

Author:
Ha, Y.W.; Stack, R.J.; Hespell, R.B.; Gordon, S.H.; Bothast, R.J.
Source:
Applied and environmental microbiology 1991 v.57 no.7 pp. 2016-2020
ISSN:
0099-2240
Subject:
Butyrivibrio fibrisolvens; rumen bacteria; strains; polysaccharides; rheological properties
Abstract:
... Most strains of Butyrivibrio fibrisolvens are known to produce extracellular polysaccharides (EPs). However, the rheological and functional properties of these EPs have not been determined. Initially, 26 strains of Butyrivibrio were screened for EP yield and apparent viscosities of cell-free supernatants. Yields ranged from < 1.0 to 16.3 mg per 100 mg of glucose added to the culture. Viscosities r ...
Handle:
10113/24370
PubMed:
1892390
PubMed Central:
PMC183514

10. Fermentaiton of xylans by Butyrivibrio fibrisolvens and Thermoanaerobacter strain B6A: utilization of uronic acids and xylanolytic activities

Author:
Hespell, R.B.
Source:
Current microbiology 1992 v.25 no.4 pp. 189-195
ISSN:
0343-8651
Subject:
xylan 1,4-beta-xylosidase; fermentation; xylan; Butyrivibrio fibrisolvens; monosaccharides; xylanolytic microorganisms; uronic acids; enzyme activity; thermophilic bacteria; rumen bacteria; alpha-N-arabinofuranosidase
Abstract:
... Butyrivibrio fibrisolvens and Thermoanaerobacter strain B6A are xylanolytic anaerobes isolated from rumen and geothermal sources respectively. Both organisms fermented larchwood xylan, oatspelt xylan, or 4-O-methylglucuronoaxylan, extensively utilizing both the monosaccharide (glucose, xylose, arabinose) and uronic acid components. Citrus pectin or polygalacturonate also supported growth of both o ...
Handle:
10113/24528

11. Genetic transformation of the ruminal bacteria Butyrivibrio fibrisolvens and Streptococcus bovis by electroporation

Author:
Whitehead, T.R.
Source:
Letters in applied microbiology 1992 v.15 no.5 pp. 186-189
ISSN:
0266-8254
Subject:
Streptococcus bovis; Butyrivibrio fibrisolvens; genetic transformation; electroporation; plasmids
Abstract:
... Electroporation methods for introduction of plasmid DNA into the ruminal bacteria Butyrivibrio fibrisolvens and Streptococcus bovis were developed. Electroporation of the strictly anaerobic B. fibrisolvens was carried out in an anaerobic glovebox with a buffer of 10% (v/v) glycerol an 1 mM MgCl-2 in distilled water. Streptococcus bovis electroporation could be carried out aerobically with a buffer ...
Handle:
10113/24530
DOI:
10.1111/j.1472-765X.1992.tb00759.x

12. Interaction of ruminal bacteria in the production and utilization of maltooligosaccharides from starch

Author:
Cotta, M.A.
Source:
Applied and environmental microbiology 1992 v.58 no.1 pp. 48-54
ISSN:
0099-2240
Subject:
Selenomonas ruminantium; Butyrivibrio fibrisolvens; starch; Prevotella ruminicola; hydrolysis; Streptococcus; rumen bacteria
Abstract:
... The degradation and utilization of starch by three amylolytic and one nonamylolytic species of ruminal bacteria were studied. Pure cultures of Streptococcus bovis JB1, Butyrivibrio fibrisolvens 49, and Bacteroides ruminicola D31d rapidly hydrolyzed starch and maltooligosaccharides accumulated. The major starch hydrolytic products detected in S. bovis cultures were glucose, maltose, maltotriose, an ...
Handle:
10113/24428
PubMed:
1539992
PubMed Central:
PMC195171

13. Purification and characterization of an alpha-L-arabinofuranosidase from Butyrivibrio fibrisolvens GS113

Author:
Hespell, R.B.; O'Bryan, P.J.
Source:
Applied and environmental microbiology 1992 v.58 no.5 pp. 1082-1088
ISSN:
0099-2240
Subject:
metabolism; xylan; Butyrivibrio fibrisolvens; enzymes; cytoplasm; pH; biodegradation; kinetics; temperature; enzyme activity
Abstract:
... An alpha-L-arabinofuranosidase (EC 3.2.1.55) was purified from the cytoplasm of Butyrivibrio fibrisolvens GS113. The native enzyme had an apparent molecular mass of 240 kDa and was composed of eight polypeptide subunits of 31 kDa. The enzyme displayed an isoelectric point of 6.0, a pH optimum of 6.0 to 6.5, a pH stability of 4.0 to 8.0, and a temperature optimum of 45 degrees C and was stable to 5 ...
Handle:
10113/24446

14. Characterization of the cell wall of Butyrivibrio species

Author:
Hespell, R.B.; Kato, K.; Costerton, J.W.
Source:
Canadian journal of microbiology 1993 v.39 no.10 pp. 912-921
ISSN:
0008-4166
Subject:
Butyrivibrio fibrisolvens; cell wall components; rumen bacteria
Abstract:
... Most Butyrivibrio strains have been isolated from the gastrointestinal tract of animals and have been classified as Butyrivibrio fibrisolvens. A few strains isolated from human feces are designated as Butyrivibrio crossatus, the other species in this genus. Butyrivibrio fibrisolvens strains are anaerobic, curved rods that produce butyrate, but numerous studies have shown that these strains display ...
Handle:
10113/24682
DOI:
10.1139/m93-139
PubMed:
8261331

15. Digestion of cell-wall monosaccharides of ryegrass and alfalfa hays by the ruminal bacteria Fibrobacter succinogenes and Butyrivibrio fibrisolvens

Author:
Miron, J.; Ben-Ghedalia, D.
Source:
Canadian journal of microbiology 1993 v.39 no.8 pp. 780-786
ISSN:
0008-4166
Subject:
monosaccharides; Butyrivibrio fibrisolvens; cell walls; alfalfa; rumen fermentation; alfalfa hay; rumen bacteria
DOI:
10.1139/m93-115
PubMed:
8221378

16. Digestion of structural polysaccharides of Panicum and vetch hays by the rumen bacterial strains Fibrobacter succinogenes BL2 and Butyrivibrio fibrisolvens D1

Author:
Miron, J.; Ben-Ghedalia, D.
Source:
Applied microbiology and biotechnology 1993 v.39 no.6 pp. 756-759
ISSN:
0175-7598
Subject:
Bacteroides; Butyrivibrio fibrisolvens; rumen bacteria; Panicum; Vicia; hay; polysaccharides; digestion; ultrastructure; cell wall components
Abstract:
... The rumen bacterial strains Fibrobacter succinogenes BL2 and Butyrivibrio fibrisolvens D1, were grown in monocultures and pair combination on cell walls (CW) of two tropical hays: Panicum (grass) and vetch (legume), and their ability to solubilize and utilize CW structural carbohydrate was determined. With respect to both substrates, F. succinogenes BL2 was a better solubilizer of CW carbohydrate ...
DOI:
10.1007/BF00164462

17. Utilization of xylooligosaccharides by selected ruminal bacteria

Author:
Cotta, M.A.
Source:
Applied and environmental microbiology 1993 v.59 no.11 pp. 3557-3563
ISSN:
0099-2240
Subject:
Selenomonas ruminantium; carbohydrate metabolism; xylan; Butyrivibrio fibrisolvens; xylanolytic microorganisms; Ruminococcus albus; oligosaccharides; rumen bacteria
Abstract:
... The ability of ruminal bacteria to utilize xylooligosaccharides was examined. Xylooligosaccharides were prepared by partially hydrolyzing oat spelt xylan in phosphoric acid. This substrate solution was added (0.2%, wt/vol) to a complex medium containing yeast extract and Trypticase that was inoculated with individual species of ruminal bacteria, and growth and utilization were monitored over time. ...
Handle:
10113/25002
PubMed:
8285663
PubMed Central:
PMC182498

18. Analysis of guanine plus cytosine contents of DNA of Butyrivibrio fibrisolvens by high performance liquid chromatography (HPLC)

Author:
Nakatani, M.; Kohda, C.; Inamoto, T.; Nakai, Y.; Ogimoto, K.
ISSN:
1011-2367
Subject:
Butyrivibrio fibrisolvens; DNA; guanine; cytosine; volatile fatty acids; butyric acid
DOI:
10.5713/ajas.1994.509

19. Expression of the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene together with the Erwinia pectate lyase and polygalacturonase genes in Saccharomyces cerevisiae

Author:
Van Rensburg, P.; Van Zyl, W.H.
Source:
Current genetics 1994 v.27 no.1 pp. 17-22
ISSN:
0172-8083
Subject:
Pectobacterium carotovorum; gene transfer; genetic transformation; pectate lyase; secretion; Butyrivibrio fibrisolvens; polygalacturonase; cellulases; Saccharomyces cerevisiae; gene expression; Erwinia chrysanthemi; enzyme activity
Abstract:
... Recombinant Saccharomyces cerevisiae strains capable of simultaneous secretion of bacterial glucanase and pectinase enzymes have been developed. The Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene (end1), the Erwinia chrysanthemi pectate lyase gene (pelE) and E. carotovora polygalacturonase gene (peh1) were each inserted between a yeast expression-secretion cassette and yeast gene terminato ...
DOI:
10.1007/BF00326573
PubMed:
7750141

20. A defined medium for rumen bacteria and identification of strains impaired in de novo biosynthesis of certain amino acids

Author:
Nili, N.; Brooker, J.D.
Source:
Letters in applied microbiology 1995 v.21 no.2 pp. 69-74
ISSN:
0266-8254
Subject:
rumen bacteria; Butyrivibrio fibrisolvens; anaerobes; Streptococcus bovis; Selenomonas ruminantium; culture media; amino acid metabolism; methionine; cysteine; strain differences; nutrient availability; ammonium chloride; nutrient requirements; glutamate dehydrogenase
DOI:
10.1111/j.1472-765X.1995.tb01009.x
PubMed:
7639995